LOCUS       DACETN010000001        89790 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DACETN010000001 DACETN010000000
VERSION     DACETN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.001"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            216..734
                     /locus_tag="IXF98_000001"
     CDS             216..734
                     /locus_tag="IXF98_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1554347.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            862..1545
                     /locus_tag="IXF98_000002"
     CDS             862..1545
                     /locus_tag="IXF98_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1554348.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1854..1927
                     /locus_tag="IXF98_000003"
     tRNA            1854..1927
                     /locus_tag="IXF98_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1888..1890,aa:Arg,seq:tct)
     gene            2086..2445
                     /locus_tag="IXF98_000004"
     CDS             2086..2445
                     /locus_tag="IXF98_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1554349.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2568..4199
                     /locus_tag="IXF98_000005"
     CDS             2568..4199
                     /locus_tag="IXF98_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1554350.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4261..4902)
                     /locus_tag="IXF98_000006"
     CDS             complement(4261..4902)
                     /locus_tag="IXF98_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1554351.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5184..5492
                     /gene="rplU"
                     /locus_tag="IXF98_000007"
     CDS             5184..5492
                     /gene="rplU"
                     /locus_tag="IXF98_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1554352.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5519..5863
                     /locus_tag="IXF98_000008"
     CDS             5519..5863
                     /locus_tag="IXF98_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1554353.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5876..6169
                     /gene="rpmA"
                     /locus_tag="IXF98_000009"
     CDS             5876..6169
                     /gene="rpmA"
                     /locus_tag="IXF98_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1554354.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6576..9410
                     /locus_tag="IXF98_000010"
     CDS             6576..9410
                     /locus_tag="IXF98_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1554355.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9601..10092
                     /locus_tag="IXF98_000011"
     CDS             9601..10092
                     /locus_tag="IXF98_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1554356.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10195..11184
                     /gene="manX"
                     /locus_tag="IXF98_000012"
     CDS             10195..11184
                     /gene="manX"
                     /locus_tag="IXF98_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1554357.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11217..12020
                     /locus_tag="IXF98_000013"
     CDS             11217..12020
                     /locus_tag="IXF98_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1554358.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12041..12955
                     /locus_tag="IXF98_000014"
     CDS             12041..12955
                     /locus_tag="IXF98_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1554359.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13038..13412
                     /locus_tag="IXF98_000015"
     CDS             13038..13412
                     /locus_tag="IXF98_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1554360.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13551..13796
                     /locus_tag="IXF98_000016"
     CDS             13551..13796
                     /locus_tag="IXF98_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1554361.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13968..15032
                     /locus_tag="IXF98_000017"
     CDS             13968..15032
                     /locus_tag="IXF98_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1554362.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15278..15703
                     /locus_tag="IXF98_000018"
     CDS             15278..15703
                     /locus_tag="IXF98_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1554363.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15696..16145
                     /gene="nusB"
                     /locus_tag="IXF98_000019"
     CDS             15696..16145
                     /gene="nusB"
                     /locus_tag="IXF98_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1554364.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16242..17096
                     /locus_tag="IXF98_000020"
     CDS             16242..17096
                     /locus_tag="IXF98_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1554365.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17097..18434
                     /locus_tag="IXF98_000021"
     CDS             17097..18434
                     /locus_tag="IXF98_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1554366.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18439..18663
                     /locus_tag="IXF98_000022"
     CDS             18439..18663
                     /locus_tag="IXF98_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1554367.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18665..19555
                     /locus_tag="IXF98_000023"
     CDS             18665..19555
                     /locus_tag="IXF98_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1554368.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19564..20382
                     /locus_tag="IXF98_000024"
     CDS             19564..20382
                     /locus_tag="IXF98_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1554369.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20489..20938
                     /locus_tag="IXF98_000025"
     CDS             20489..20938
                     /locus_tag="IXF98_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1554370.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20956..22638
                     /gene="recN"
                     /locus_tag="IXF98_000026"
     CDS             20956..22638
                     /gene="recN"
                     /locus_tag="IXF98_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1554371.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22693..22809)
                     /locus_tag="IXF98_000027"
     CDS             complement(22693..22809)
                     /locus_tag="IXF98_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1554372.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22882..23838)
                     /locus_tag="IXF98_000028"
     CDS             complement(22882..23838)
                     /locus_tag="IXF98_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1554373.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            24009..24377
                     /locus_tag="IXF98_000029"
     CDS             24009..24377
                     /locus_tag="IXF98_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1554374.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24547..24978
                     /gene="mraZ"
                     /locus_tag="IXF98_000030"
     CDS             24547..24978
                     /gene="mraZ"
                     /locus_tag="IXF98_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1554375.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24999..25958
                     /gene="rsmH"
                     /locus_tag="IXF98_000031"
     CDS             24999..25958
                     /gene="rsmH"
                     /locus_tag="IXF98_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1554376.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25963..26364
                     /gene="ftsL"
                     /locus_tag="IXF98_000032"
     CDS             25963..26364
                     /gene="ftsL"
                     /locus_tag="IXF98_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1554377.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26365..28557
                     /locus_tag="IXF98_000033"
     CDS             26365..28557
                     /locus_tag="IXF98_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1554378.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28583..29545
                     /locus_tag="IXF98_000034"
     CDS             28583..29545
                     /locus_tag="IXF98_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1554379.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29559..30920
                     /locus_tag="IXF98_000035"
     CDS             29559..30920
                     /locus_tag="IXF98_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1554380.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30946..32034
                     /gene="murG"
                     /locus_tag="IXF98_000036"
     CDS             30946..32034
                     /gene="murG"
                     /locus_tag="IXF98_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1554381.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32049..33269
                     /locus_tag="IXF98_000037"
     CDS             32049..33269
                     /locus_tag="IXF98_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1554382.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33425..34750
                     /gene="ftsA"
                     /locus_tag="IXF98_000038"
     CDS             33425..34750
                     /gene="ftsA"
                     /locus_tag="IXF98_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1554383.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34773..36014
                     /gene="ftsZ"
                     /locus_tag="IXF98_000039"
     CDS             34773..36014
                     /gene="ftsZ"
                     /locus_tag="IXF98_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1554384.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36031..36708
                     /locus_tag="IXF98_000040"
     CDS             36031..36708
                     /locus_tag="IXF98_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1554385.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36722..37267
                     /locus_tag="IXF98_000041"
     CDS             36722..37267
                     /locus_tag="IXF98_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554386.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKAKLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAP
                     MTAFDTQAIDISIVPGENENE"
     gene            37529..40327
                     /gene="ileS"
                     /locus_tag="IXF98_000042"
     CDS             37529..40327
                     /gene="ileS"
                     /locus_tag="IXF98_000042"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1554387.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40376..41902)
                     /locus_tag="IXF98_000043"
     CDS             complement(40376..41902)
                     /locus_tag="IXF98_000043"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1554388.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41917..42564)
                     /locus_tag="IXF98_000044"
     CDS             complement(41917..42564)
                     /locus_tag="IXF98_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1554389.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42748..43077)
                     /locus_tag="IXF98_000045"
     CDS             complement(42748..43077)
                     /locus_tag="IXF98_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1554390.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43254..43982
                     /gene="sfsA"
                     /locus_tag="IXF98_000046"
     CDS             43254..43982
                     /gene="sfsA"
                     /locus_tag="IXF98_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1554391.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43998..45011
                     /locus_tag="IXF98_000047"
     CDS             43998..45011
                     /locus_tag="IXF98_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1554392.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            45011..46288
                     /locus_tag="IXF98_000048"
     CDS             45011..46288
                     /locus_tag="IXF98_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1554393.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46351..49053
                     /locus_tag="IXF98_000049"
     CDS             46351..49053
                     /locus_tag="IXF98_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1554394.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49205..49522
                     /locus_tag="IXF98_000050"
     CDS             49205..49522
                     /locus_tag="IXF98_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1554395.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49552..49872
                     /locus_tag="IXF98_000051"
     CDS             49552..49872
                     /locus_tag="IXF98_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1554396.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49980..51440
                     /locus_tag="IXF98_000052"
     CDS             49980..51440
                     /locus_tag="IXF98_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1554397.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51508..51729
                     /locus_tag="IXF98_000053"
     CDS             51508..51729
                     /locus_tag="IXF98_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554398.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51760..51942
                     /locus_tag="IXF98_000054"
     CDS             51760..51942
                     /locus_tag="IXF98_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1554399.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51942..52355
                     /locus_tag="IXF98_000055"
     CDS             51942..52355
                     /locus_tag="IXF98_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1554400.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52478..53659
                     /locus_tag="IXF98_000056"
     CDS             52478..53659
                     /locus_tag="IXF98_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554401.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            54048..54121
                     /locus_tag="IXF98_000057"
     tRNA            54048..54121
                     /locus_tag="IXF98_000057"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54082..54084,aa:Arg,seq:cct)
     gene            complement(54190..55329)
                     /locus_tag="IXF98_000058"
     CDS             complement(54190..55329)
                     /locus_tag="IXF98_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1554402.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(55628..56263)
                     /locus_tag="IXF98_000059"
     CDS             complement(55628..56263)
                     /locus_tag="IXF98_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554403.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(56376..57011)
                     /locus_tag="IXF98_000060"
     CDS             complement(56376..57011)
                     /locus_tag="IXF98_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1554404.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(57045..57506)
                     /locus_tag="IXF98_000061"
     CDS             complement(57045..57506)
                     /locus_tag="IXF98_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1554405.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(57636..58067)
                     /locus_tag="IXF98_000062"
     CDS             complement(57636..58067)
                     /locus_tag="IXF98_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1554406.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(58085..58405)
                     /locus_tag="IXF98_000063"
     CDS             complement(58085..58405)
                     /locus_tag="IXF98_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1554407.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            58704..59480
                     /locus_tag="IXF98_000064"
     CDS             58704..59480
                     /locus_tag="IXF98_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1554408.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            59495..59698
                     /locus_tag="IXF98_000065"
     CDS             59495..59698
                     /locus_tag="IXF98_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1554409.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            59714..60052
                     /locus_tag="IXF98_000066"
     CDS             59714..60052
                     /locus_tag="IXF98_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1554410.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            60039..60218
                     /locus_tag="IXF98_000067"
     CDS             60039..60218
                     /locus_tag="IXF98_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554411.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(60261..60731)
                     /locus_tag="IXF98_000068"
     CDS             complement(60261..60731)
                     /locus_tag="IXF98_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554412.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            60818..61516
                     /locus_tag="IXF98_000069"
     CDS             60818..61516
                     /locus_tag="IXF98_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1554413.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            61694..62035
                     /locus_tag="IXF98_000070"
     CDS             61694..62035
                     /locus_tag="IXF98_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554414.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            62028..62699
                     /locus_tag="IXF98_000071"
     CDS             62028..62699
                     /locus_tag="IXF98_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1554415.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            62705..63391
                     /locus_tag="IXF98_000072"
     CDS             62705..63391
                     /locus_tag="IXF98_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554416.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            63394..64143
                     /locus_tag="IXF98_000073"
     CDS             63394..64143
                     /locus_tag="IXF98_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1554417.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            64155..64424
                     /locus_tag="IXF98_000074"
     CDS             64155..64424
                     /locus_tag="IXF98_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554418.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            64586..64888
                     /locus_tag="IXF98_000075"
     CDS             64586..64888
                     /locus_tag="IXF98_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554419.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            64885..65046
                     /locus_tag="IXF98_000076"
     CDS             64885..65046
                     /locus_tag="IXF98_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1554420.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            65043..65348
                     /locus_tag="IXF98_000077"
     CDS             65043..65348
                     /locus_tag="IXF98_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554421.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            65348..65704
                     /locus_tag="IXF98_000078"
     CDS             65348..65704
                     /locus_tag="IXF98_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1554422.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            65664..65909
                     /locus_tag="IXF98_000079"
     CDS             65664..65909
                     /locus_tag="IXF98_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554423.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            65906..66325
                     /locus_tag="IXF98_000080"
     CDS             65906..66325
                     /locus_tag="IXF98_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1554424.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            66322..66879
                     /locus_tag="IXF98_000081"
     CDS             66322..66879
                     /locus_tag="IXF98_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1554425.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            66876..67172
                     /locus_tag="IXF98_000082"
     CDS             66876..67172
                     /locus_tag="IXF98_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554426.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            67249..67662
                     /locus_tag="IXF98_000083"
     CDS             67249..67662
                     /locus_tag="IXF98_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1554427.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(68120..68395)
                     /locus_tag="IXF98_000084"
     CDS             complement(68120..68395)
                     /locus_tag="IXF98_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554428.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            68849..69055
                     /locus_tag="IXF98_000085"
     CDS             68849..69055
                     /locus_tag="IXF98_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554429.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            69251..69418
                     /locus_tag="IXF98_000086"
     CDS             69251..69418
                     /locus_tag="IXF98_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1554430.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            69444..69788
                     /locus_tag="IXF98_000087"
     CDS             69444..69788
                     /locus_tag="IXF98_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1554431.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            69793..70074
                     /locus_tag="IXF98_000088"
     CDS             69793..70074
                     /locus_tag="IXF98_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554432.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            70177..70491
                     /locus_tag="IXF98_000089"
     CDS             70177..70491
                     /locus_tag="IXF98_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1554433.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            70469..72163
                     /locus_tag="IXF98_000090"
     CDS             70469..72163
                     /locus_tag="IXF98_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1554434.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            72183..73361
                     /locus_tag="IXF98_000091"
     CDS             72183..73361
                     /locus_tag="IXF98_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1554435.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            73324..74010
                     /locus_tag="IXF98_000092"
     CDS             73324..74010
                     /locus_tag="IXF98_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1554436.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            74010..75170
                     /locus_tag="IXF98_000093"
     CDS             74010..75170
                     /locus_tag="IXF98_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1554437.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            75180..76055
                     /locus_tag="IXF98_000094"
     CDS             75180..76055
                     /locus_tag="IXF98_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554438.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            76052..76363
                     /locus_tag="IXF98_000095"
     CDS             76052..76363
                     /locus_tag="IXF98_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554439.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            76353..76706
                     /locus_tag="IXF98_000096"
     CDS             76353..76706
                     /locus_tag="IXF98_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1554440.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            76696..77097
                     /locus_tag="IXF98_000097"
     CDS             76696..77097
                     /locus_tag="IXF98_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554441.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            77090..77494
                     /locus_tag="IXF98_000098"
     CDS             77090..77494
                     /locus_tag="IXF98_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554442.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            77506..78114
                     /locus_tag="IXF98_000099"
     CDS             77506..78114
                     /locus_tag="IXF98_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1554443.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            78134..78496
                     /locus_tag="IXF98_000100"
     CDS             78134..78496
                     /locus_tag="IXF98_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554444.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            78499..78681
                     /locus_tag="IXF98_000101"
     CDS             78499..78681
                     /locus_tag="IXF98_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554445.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            78698..82129
                     /locus_tag="IXF98_000102"
     CDS             78698..82129
                     /locus_tag="IXF98_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1554446.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            82180..82917
                     /locus_tag="IXF98_000103"
     CDS             82180..82917
                     /locus_tag="IXF98_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1554447.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            82927..85218
                     /locus_tag="IXF98_000104"
     CDS             82927..85218
                     /locus_tag="IXF98_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554448.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            85242..87368
                     /locus_tag="IXF98_000105"
     CDS             85242..87368
                     /locus_tag="IXF98_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554449.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            87531..87977
                     /locus_tag="IXF98_000106"
     CDS             87531..87977
                     /locus_tag="IXF98_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554450.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            87979..88116
                     /locus_tag="IXF98_000107"
     CDS             87979..88116
                     /locus_tag="IXF98_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1554451.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            88154..88447
                     /locus_tag="IXF98_000108"
     CDS             88154..88447
                     /locus_tag="IXF98_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554452.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            88444..88668
                     /locus_tag="IXF98_000109"
     CDS             88444..88668
                     /locus_tag="IXF98_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1554453.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            88665..89690
                     /locus_tag="IXF98_000110"
     CDS             88665..89690
                     /locus_tag="IXF98_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1554454.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        30388 a        14614 c        18862 g        25926 t
ORIGIN      
        1 acataaaggt attcctttct aaaacttgta atgttagggt aaacgttttt taaactaacg
       61 tcaattattt ataataaaaa atgagataaa gatgaggaaa tagaattaga aacatataat
      121 tattaaaggg aaacattttt acagctataa tttgaaaaaa atttatcgtt attatatgat
      181 taagaaggat tgaataaaga aagaagttga aaacgatacc tgattacaaa aaaataactt
      241 ttataagttc tataacgttg attatatttt taattcttat tcttctcgtc gtcttggttc
      301 cccgaatttt tggtttgact ccttatatta ttgaggacaa agaaatgagt ccatattatg
      361 cacaaggaaa tctgatatat gtacgaccga aaaatacaaa tgagatttta gtcggagata
      421 ttattacata ttatgaaaat tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg
      481 aaaacaacca cgaagatttt tatacaaaag ctgatacaaa gacttatatt gagcctggag
      541 tagtaaaaaa gagaaatatt atcggtagtc ccatctttca gattccatat attggactag
      601 tcctttcaaa aacggcattt gcatggattc aggggttatt ctttacgatt gctttttgcc
      661 taacaggaat cacagcatgg aatacctttg tagagttgaa aaagaagcgg agacgaacac
      721 agaaattttt gtaagaatgt taaaaacata aatgtattcc agagatccaa cgactcttct
      781 gaacatgaaa cacgagaaaa cactcatgca gcttgtagat ttagtcagtt gttcgtataa
      841 tgtgtgaaga ggaggagtag tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa
      901 gaactattgg attttatgcg tacccagcaa aaaaaattac cgggtgagct aggcaaactg
      961 gaagaagaag ctcatgtagc agaagtacca gttattcccc atgaaacagt tgtatttttg
     1021 aaatttttac ttggacagct tcagcctgaa cgaatcttgg agatcggagc agctatcggt
     1081 ttttcatcaa gtgtcatggc cacgacaatc ggagaaaacg ctcatgttac gacaattgat
     1141 cgtttcgatg tcatgatcga gaaagctaaa aaaaactatg aacgtctagg actgacagat
     1201 aaagtgactt tgcttgaagg tcaagcagca gatatcttgc cggagttgtc tggtccgtat
     1261 gactttattt ttatggatag tgcaaaatca aaatatattg aatttttacc tgaatgctta
     1321 cgtttgttaa gaaaaggtgg cgttttgatg gtagatgaca tcttccaagg aggaacgatc
     1381 ttgctcccag atgaagagat tccccgtggc aaacgagcta tccatcgtaa attaaatgaa
     1441 tttcttcgtg ttgtgatgga tcatcccgat ttgacatcga caatcttgcc attaggtgat
     1501 ggtgtgatct taatgacaaa ggaatcagaa acaatcgagt tgtgatacat gattatggca
     1561 gcctgtagat aatcgattga atcgagggct caattcgaac ttgatgtaaa agtttctata
     1621 tttgtaagtc aattaaagga tacaggaaat ctaaataaac aggagtagca tgaagtcaat
     1681 gtagctcctg ttttcaattg ataaccttag aacagctgaa ggagaatgtc tcgttttaaa
     1741 agattgattt agtagttggg ccaaaataat tcattatttg ctaattaaat aactatttcg
     1801 tattgacaga agcaatttaa ttatctataa ttattgatgt tccgctgatt cttgtcatag
     1861 tagctcagct ggatagagca tccgccttct aagcggacgg tcgggggttc gaatccctcc
     1921 tgtgacgcta aaaaaccgtt agaaactgag gtttctaacg gtttttcttt gttttttgtg
     1981 ccaatattgt ttgttttcaa aggcaaataa aataaaaaaa catccttatc gagtacaatt
     2041 ataaggagca aaaaaataaa actcatcaga aaagaggaag aagatatgac gatactatct
     2101 agaattttag tgactctagt tgcaattgaa tttttttata tcatgtatat cgaaacggtg
     2161 agaactgatt ctgacacgac aagtagggtt tttaaaatga gtaaagaaga gttaagcaga
     2221 aaatctgttc aaacattgtt taagaaccaa ggagtgtaca atggactatt aggagtggga
     2281 ctactttatg gagcttatct ttcttccgca tctaaagaaa tcacaagtat gctattaata
     2341 tctatttttt ttgttgcatt atatggcagt ttgactagcg atcgttggat tttactcaaa
     2401 caaggtgggc tagctctgtt ggcattgctt tctattttct tataaatata gaaaaaataa
     2461 gaatttatct aacaggaaac tttgtgaaaa atattacttt atctcatgaa atatgatatt
     2521 ctaatgaatg gtattttaga aaatttcatg aaaaaggagt tagataggtg ttcaaaaatt
     2581 atgtttcctc actaaaaaaa gaattttcag gttatgatct ttcaagattt cagaaagatc
     2641 ttttggctgg gatcacagta gctgctgtag cattgccttt agccttggct tttggggtat
     2701 caagcggagc tgatgcggcc gcaggtttga ttacggctat cattgccggt atcgttattg
     2761 gcggtttatc aggcggtttt tatcaaatat caggtccgac aggtgcgatg gcagctatct
     2821 tgatgtctat tgcggcaaca aagggaatgc aaggtgtatt gcttgctact tttttagcgg
     2881 gtctattttt acttgcagca ggacttttcc gattaggaac actgacttct tttatcccag
     2941 cacccgtaat tacggggttt acttcaggta ttgctatcat tattgcatta ggtcagatcg
     3001 ataatttatt tggtgttcac tcggaaggta caaacgttat tgaaaaacta agtagttatc
     3061 aaacgatagg atttccgata aacacagcct ctttgatcat gggagggcta gtcattttag
     3121 ggatgtttat ctatccaaaa aaatgggcga aacgaatgcc tagttcgctg ctggctatca
     3181 ttttggcaac agcagttatg ctattgttcc atttaccagt agcaactgtg ggtaagattc
     3241 cacaaacatt gatcagtagt aatcgtttga atatgggaga ttttagcttt tctgccttgc
     3301 aacaagtggc agttccagct attagcattg ctttattagg aatgattgaa agcttgctat
     3361 gtggtgcttc ggctggccga atggcaaata agcccttaga tagcaatcaa gaattaatgg
     3421 cgcaaggaat tggaaatctg ttgcttcctt tctttggcgg gattcctgct actgcggcca
     3481 tcgcccgtac aagcgtggcc atcaaatctg gtgcacaaac tagattagct ggtatgatac
     3541 atgcagttgt cttactgctc tccatgcttg tttttgcgcc agtgatgtca cagattccaa
     3601 tgccagcact tgcaggtgtt ttgattgtga cggcttggcg tatgaatgaa tgggaaacga
     3661 tcaaggagct atttactaat aaatattggt cagcagttct attgtttatc cttacgatga
     3721 tttgtacagt tatctttgat ttaagtattg ctatcgtgat cggggtgatc ggcggctgtg
     3781 tattttttgt tgtcaaaagc gctgctatca cgatttcatt agaaacgatc gattggcaaa
     3841 gaatggggct gccagaaacg gaaaagctga ataactggac agtagtgtat attagtggtc
     3901 cactgttttt tatgtcttca gaaaaactca aaaataaatt ggaagaacta aatcatcacg
     3961 atggtgtcat tttctccatg cgaggcgtac caagtattga tttgacggca cgtgcagttt
     4021 ttgatgaatt tcaagaaaaa gcagagcaaa gaggacaaac gattatctat acttccttgc
     4081 agccagaagt agaaaaacaa ctttctcact tgtgggaaca tacattcacg catcaacatc
     4141 aaacagttgc tcatgcgttg acagctttac atgcgaaata tactgcaatt agccattaaa
     4201 acaatgaaaa agaacagaca agactgtaaa ctgaagattt tatggtcttg tctgttcttt
     4261 ttaatattgc tgtaaaagtg aagctagtac ttgccagtct tgatggataa caggtacaag
     4321 ggagcgattg tagaaaatag ctgctggatg aaaaagtgga agaattcgat agtttttttc
     4381 tgaccattcg taggcatcct tttgttctgt taattgtaag acaggccctg tatagagttg
     4441 accatgattt ttggtaaccg tatattcttt gccaagtaaa cgttgaagac caatgtttcc
     4501 aagtgtcgca attaattttg gctggacctt catcagttcg taatccaata ttggtgcatg
     4561 cgcaaatact tcggaacgag taggtgtccg atttggataa acagtttctg actgttgtgt
     4621 ccgtttattg atccgatgag tcacgcgata cgggcggctc cttacagcac tggtgatata
     4681 cacctcttct cgtgttaagc cagttgaaga aagtgcctgc atcaattctt ttccagcttg
     4741 accgctaaaa ggaacatgcg tatcaatctc tgttttccct ggggcttctc ccacgagcat
     4801 cagtttagga tgaagaggtc cttgcccagc gataaatcct tctacaggac ggccgttcat
     4861 tcttaaagaa gcttcttgta tcagttcttt tgggtattcc atttatgctc ctcctttttc
     4921 ttttattata agaataaaag aaacacaatg cgactatttt atttggtaga actgtgagga
     4981 tagaaaagca gaagtattgc ttgaattttt gttttacttt ttgattttaa atttagttga
     5041 ctaacggttg ctgttctggt aagctagtac tgttgtaatt gtggactcca cagctacaac
     5101 cgctcagaac aagtaataag cacagcatat gctgtcactt cggatggcga gtctaagtat
     5161 atcagaagga ggtgctttat agcatgtacg caattattaa aactggtggt aaacaagtaa
     5221 aagttgaagt gggacaagca atctatgttg aaaaacttga cgttgaagca ggagaaaaag
     5281 ttgtttttga cgaagttatc ttagtaggcg gagaatctac aaaagtaggc gctccaactg
     5341 ttgcaggtgc aactgttgaa ggtactgttg aaaaacacgg caaacaaaag aaagtcgtta
     5401 ctttcaaata caaacctaaa aaacattcac accgtaaaca aggtcatcgt caaccatata
     5461 caaaagtggt tatcgacgcg atcaacgctt aatagcgttt gagaaagaag gcctatatat
     5521 gatcaaagga acatttaagc gaaatgacgc aggtcagatc gtttcattca ctttgaccgg
     5581 gcatgctgat gctggtcctt atggaagtga tatcgtttgt gcgggtgttt ctgcgttagc
     5641 gatcagcact gtcaacggga ttgcctcatt agccggtttt gagccgattg ttgaaatgaa
     5701 tgaagaagaa ggcggttatc tttataccga agtcacttca ggaatgacac aagaacaaaa
     5761 caatattgct gagattctat tagaaaatct tttattagga ttacaatcga tagaagctga
     5821 aaactcagaa tatatccaaa ttaaaacaat taatgataaa taggaggtgc agaccatgtt
     5881 gttatctatg aatctacaat tatttgccca caaaaaaggt ggcggttcta catctaacgg
     5941 ccgtgactca gaatcaaaac gtctaggtgc taaacgtgct gatggacaaa cagttacagg
     6001 tggatcaatt ctttaccgtc aacgcggtac aaaaatctat ccaggtgtaa acgtgggtat
     6061 cggtggagac gacactttat ttgcaaaagt tgatggcgta gtacgtttcg aacgtaaagg
     6121 ccgtaacaaa aaacaagtgt ctgtttatcc agtagctcaa gaagcttaat tgaatcttat
     6181 gattaaagag gttgtgacaa gacttttgtc acagcctttt ttgtttataa aaataaaagt
     6241 tgtagtggag gctacgaagt ttactttgtc tttttacact taatattttt ttgccaattt
     6301 ggaatcagga tgttttgata actatcgttt tcaattgatg gaagctatat tgtttgctat
     6361 cagtgaaata attatatggt aaacagtgtt ctttttcttg tttccattat aaaaatcgta
     6421 tcgggatttt tcgttctttt aaattgaaca tttctatcta tccgcgtgtt atcaacaaaa
     6481 caaaatcagg taaatagttg acgaaacaga aataaataca tagtattatg ttttgaagcc
     6541 taagtacaat agtctataga aaaggagcga taccattggc aaaacgcatt gaccgaattt
     6601 atatgtatgt caaagaaaaa accgctcatt taacaaaggc agaatatgat caaggagtaa
     6661 caacacagga aattgctgaa gtgttaggga ttcagcgaac aaatagcagt aaagatctga
     6721 atcagctagt gcgcgaagga aaactgataa agacagacgg acgaccagta cgttacatat
     6781 atcaaacact cgttacgcac gtaaagccag ttacaaaaca tgtagtcagt tacaaagaaa
     6841 cgccggtctc tgaagaaaaa aatacactcc aaatagatac taaggatatc tttgcaaaaa
     6901 tcattggagc taacggcagc atgaaaaatt cagtggaaca ggcaaaagct gcgattttgt
     6961 atccgccaag aggattaaat tgtttgatta caggacctac cggatcagga aagacttatt
     7021 ttgcacacgc gatgtatcat tttgcaaaag cgcatcatgt aatagatgag gaaacggaac
     7081 tgattgtttt caattgtgct gattatgcca ataaccctga gcttttgatg agtcatttat
     7141 ttgggtacgc aaaaggtgct tttacaggcg cagatgaaga aaaaatgggg attatcgatc
     7201 aagcggatgg cggtatgctg tttttggatg agattcaccg gttgccgcct gaaggtcaag
     7261 aaatgatctt ttactttatg gatcatggaa cttacagtcg tcttggggaa acaacgaaat
     7321 cccatgaagc aaatgtacgg atcgtaggtg cgaccacaga agacccaggt tcttctctat
     7381 tagaaacttt tgttcggcgt atcccaatca atatcaaact tccggctttt gaaaaacgtc
     7441 ctgcaaatga gaaagtcgat ttagtaaaaa tcatgattgc ccacgaagca aaccgcacgc
     7501 agcgaaagat ttctttgaca gaagatgtag caaaagcatt gatcggcagt gtgacctatg
     7561 gaaatatagg acaattgaag tcaaatatcc agttagtatg tgcacgagga tttctaaatc
     7621 atatgcaatc acccgaaatc tcgatcacaa tcgatgattt gacagaaggg attcgtagcg
     7681 gattgattca attagcaagc aaccgcagtg caatggcaga gctttctaaa atattggagc
     7741 caaagataac tgtagcacca aatgatacga tcatgaaaat ccaatcggac tcctatgagt
     7801 taccttataa tctatatgat atcatcggag ataaagcagc tttactgaag tcggatggtt
     7861 tggatcaaga agcaatcaat catttcattt ctacagatat caacatccat ctgaagtctt
     7921 tctataaaga ccatggtttt tcttttaatg cagacagcaa attagcagaa tttgtagatc
     7981 cgaaaatcat agaagtgacc aatcagattt atgtaatggt caaaagatct ttaccatatg
     8041 agtttcagca aaactttatt tatgctatga gcttgcatat cagttctttt ttgaaaaaaa
     8101 tcaacttagg agaagaacgg catacgaatg ataacatccg tgagatggct tttgattttc
     8161 cagatgaata tgctgttgcg aaagaagtgc gtagttatat cgaaacgtat tttcaagtgg
     8221 agattccaga aagtgaagac tattacttga ccgttctact cgtctcgtta cgggcaaatc
     8281 aagcaagcgg cagaatcggt gtagtcgttg cagctcacgg aaacagcaca gcaagcagta
     8341 tggtccaagt tgtccaacag ttgttagatg cagagaacgt acgagcagta gacatgcctt
     8401 tggatatgga tccgaaaaca gcattggacc gaattgaacg caatgtacaa gaagtagatg
     8461 aaggaagcgg ggtcatactc ttagtagata tggggtccct tgcgtcgttt aattcgcaaa
     8521 ttcaaagaga cactggaatt ccagtacgta ctgttgatat ggtaacaact tctctagttt
     8581 tggaaactgt acgaaaggtc tcagtattag ggacagatct agatatgttg catgaatcat
     8641 tgaaaaattt tcgaggttac gcagaaatat cttctgtaga atctgttcag gaagcaaaac
     8701 ctattcataa gaaaaaagct gttttggcta tctgcgcatc tggcgaaggt acagcacaaa
     8761 gaatcaaaga attgatcgaa cgagcggtaa acaagcgtca agaaacagaa ttagaagttc
     8821 tagctttgtc gattgtggaa gtgaaagaag aactgccagt tatccaaaaa gaatatcaga
     8881 tcatagctac tactgggatt actgatttga atatccaagc cccatttatt cctttagaac
     8941 gttttattga tcaaaatatc gaagtcattt tggatcagtt gctgatggaa tcagaattgg
     9001 aggaaactga attcatctcc ctagatgaag agtcagctaa aaatacgtgt gttgagttta
     9061 tctctgataa cttcatcttt attaatggta gtaagttaat cgatccaatg tggcaatttt
     9121 ctacacaaat ctctcagacg acgggaatcg gcgatgaaga atatggattc aaaatcaacc
     9181 tagtgatgca cacagcagga atgatcgaac gtattattcg gaatgaacca ttgactgtgg
     9241 aagaaaacga attgacaaat acaacgaatg atcctcttta tagccaattg gcagcctcag
     9301 ttgttctgtt ggaagatcag ataaaagtaa aagtgccgat agaagaaatg tattatctgc
     9361 tacgtctagt ccataatcag ttagataaaa aagaatacac tgtgccatga atacacaaaa
     9421 acgatacact tttatagtgt atcgtttttg tgtattggtt aagaatcctt taaattcaat
     9481 tagtgtatcg taaaaaagtt ggcacgcttt ttgcttatag tagtgtgtgc taggaggtgg
     9541 ttgttcacac agaaatgata ggggtttctg tgaagtagtg aggaagaagg agttttgcag
     9601 atggatattc gattggcacg tatagatgat cgtttaatcc acgggcaagt cgcaacagtt
     9661 tggtcgaagc aaacaggtgt tgaacgtatc attgtgatca gcgatgtagt cgctcaagat
     9721 aaattgagaa agtttctact aaaagaagct gcaccgccgg ggatcacagc aaatgtaata
     9781 accgtgcaga aaatgattca aatctatcat agtggattat tatcacgagc aaaagtgatg
     9841 cttttattca ccaatcccca agatgttgaa gcggttgtcc gctgcggtgt acagttgaag
     9901 tcgttgaata tcggcggtat gagttttact gaaggcaaat caatgattac caattttgtt
     9961 tcagtaaatc aacaagatat tgattccttt cgttacttga acagtcaaga agtggagtta
    10021 gagattcgaa aagtacctgc tgacaggaaa atccttttga tggaactact agcgaaaatc
    10081 aaaaaggtgt agcaagaatg gttgttggaa aaggtcaaat aattctgttg taatggttag
    10141 caattattaa tatttttgaa tttgttacaa caaaagtaaa taggaggtat aaatatggta
    10201 gggattatcc tagcaagtca tggacaattt gctgaaggaa tcttacaatc cggttcaatg
    10261 atctttggcg aacaagaaaa cgtcaaagcc gtgattttga aaccaagtga aggtccagat
    10321 gacttgagag ccaaattgga agaagcggtt gcttcttttg ataatcaaga cgaagtgtta
    10381 ttcttagttg atttatgggg cggaacaccg ttcaatcaat caaacacatt attcgaagag
    10441 cacaaagata aatgggcaat cgtcagcggt ttgaatttac caatgttgat tgaagcctac
    10501 gcatcacgct tttcaatgga atctgctcat gaaattgcag cacacatcat tgaaacagca
    10561 aaagacggtg taaaagtaaa accggaagaa ttagaaccgg cagaagcacc gaaagcagca
    10621 gttgaagacg cacaacctaa aggagcactt ccagaaggaa ctgtggtcgg agacggaaaa
    10681 atcaaatatg tattagcacg tgttgactca cgtctgttac atggacaagt tgcgacagct
    10741 tggacaaaag cagtgcaacc aaaccgtatt atcgttgttt cagatgcagt atcaaaggat
    10801 gatctgcgta agagactaat cgaacaagct gctcctccag gtgtaaaagc aaatgttatt
    10861 cctattagca aaatgatcga agtagcaaaa gaccctcgtt ttggaaatac caaagcattg
    10921 cttttatttg aaaatcctga agacgtattg acagctgttg aaggtggcgt agacatcaaa
    10981 gaattaaatg ttgggtcaat ggcgcattcg gtcggtaaag tcgttgtaag taaagtattg
    11041 tctatgggac aagaagacgt tgaagcgttt gagaagcttg aacaaaaagg tgtgaaattc
    11101 gatgtacgta aagtacctaa tgattcacgc gataacatgg atgatatttt gaaaaaagca
    11161 aaagctgaat tagcaaaagc gtaataaata atattaaaat ttataggagg cttatcatgt
    11221 ctattatttc aataatttta gtcgtattag ttgcctttct agctggtatg gaaggtattc
    11281 ttgatgaatt tcaatttcat caaccactag tggcatgtac attgatcggt ttagttaccg
    11341 gtaacctcga agctggtatc gtacttggcg gtacgcttca gatgatcgct ttaggttggg
    11401 caaatatcgg tgccgctgta gcaccagatg ctgcattagc atcagtagca tcagcaatta
    11461 ttttagtttt aggtggacaa ggtgttaaag gtgttccttc tgcgatcgcg attgccgttc
    11521 ctttggctgt tgcagggttg ttcttaacaa tgatcgtacg tacaatcgct gttccaatcg
    11581 ttcatttgat ggatgcggct gctgaagaag gaaacatccg taaggttgaa atgtggcaca
    11641 tcattgctgt atgtttacaa ggggttcgta tcgcggtccc agcagcagcg ttgctattta
    11701 tcccagcaca aactgtgcaa tcattccttg aatcaatgcc agcatggtta acagatggta
    11761 tggctatcgg tgggggaatg gtcgtagctg taggttacgc tttggtaatc aacatgatgg
    11821 ctacaaaaga agtatggcca ttcttcgtta ttggtttcgt tgtagctgca atttctcaat
    11881 taacattgat tgctctaggt gctttaggtg ttgcattagc tcttatttac ttgaaccttt
    11941 ctaaaatggg tggttcatca aataatggag gcggaagcaa ctctggtgac cctttaggcg
    12001 acattttgaa tgactattaa tcttgaagga ggaaaataaa atggcagaag aaaaaatcaa
    12061 attatcaaaa aaagatcgtt tagctgttgc gtggcgctct accttcattc aaggttcatg
    12121 gaactacgaa cgtatgcaaa acggtggctg ggcattttct atgattccag caatcaaaaa
    12181 attatacaaa acaaaagaag aacgttctgc agcattaaaa cgtcacttgg aattctttaa
    12241 tacacaccca tacattgctt ctccaatttt aggggttaca ttggctctgg aagaagaacg
    12301 tgcgaatggc gcgcctgttg atgatgtagc gattcaaggg gttaaagtag gtatgatggg
    12361 tcctttagct ggtgttggtg acccagtgtt ctggtttaca gtacgtccaa tgctaggtgc
    12421 actaggtgct tcattagcta tgggaggaaa tatccttgga ccaatcatct tcttcttagg
    12481 ttggaacatt attcgttggg cattcatgtg gtatacacaa gaatttggct acaaagctgg
    12541 ttctaaaatt actgatgacc tttctggcgg attgttacaa gacgtaacaa aaggtgcttc
    12601 aattttaggg atgttcgtat tagctgccct tgtacaaaga tgggtatcaa tcaaattctt
    12661 accagttgta tcaactgtaa aattagataa aggtgcttac atcgaatggg ataaattgcc
    12721 ttcaggtggt gaaggaatca agagtgcatt tgaacaagtt aataatggct tagccctttc
    12781 tcctgaaaag gtaacaacat tacaagacaa cttagaccaa ttgattcctg gtttagctgc
    12841 attaggatta acattgttat gtatgtggtt gcttaagaaa aaggtttctc caatcgttat
    12901 catccttggt ttattcgtag tcggtgttgt tggacacgtc atcggacttt tataatccat
    12961 aaaaatatag ctcgggcgtt gtcccgagct ttttttatat agtatactgt tcataaacaa
    13021 atatctggag ggagaaaatg gttcaatcaa tcaatacaaa agtagacttg gtaattgatg
    13081 ctacttcgtt tacgggtctg acagattacg ggcaaatcat gataggtgat aatggttttg
    13141 agttttacca tgcgcgagat agacgcaaat atatccaaat tccatgggaa gaagtggatt
    13201 atgtactagc atcagtcatg ctaaagggaa aatggattcc tcgatatgcc attcaaacga
    13261 aaaaaaatgg tacatacaca ttttcttcca aagaacctaa aaaagtgctt cgtgcgatca
    13321 gagaatatgt agatgaagat agaatggtac aatcattgag tttctttgat gtagtgaaac
    13381 gtggtattcg tagtatgttt aaacgcaagt aaaaaataaa gaaaacttct aagaggaggt
    13441 tttctttatt tttttacttt gcagaagaaa atatgaaata gcaaaaccaa ttatttgtcc
    13501 cctttaaatc ccatatgcta aaattgatta gcaaagaagg aggttatatt atgagttggt
    13561 tatggacatt gattgttgga gccgtgatcg gagcaattgc aggtgcgatc accagtaaag
    13621 gaaattcgat gggctgtgta acgaatatca ttgctggact agtcggttca agtattggac
    13681 aagcgatttt cggcgaatgg ggaccaaaag ctgcaggtat ggcacttatt ccatctattt
    13741 taggcgcagt gattttggta gcagttgtct catttttctt tggaaaacga tcataagttt
    13801 ttacaaaata gtaaactaaa aggagttgca gcgagactgt gactcctttt tggctttatt
    13861 tatttaaaat aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa
    13921 gtttgctata atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg
    13981 tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc
    14041 cgtacaatct tcgttactta acgaacttta caggtacgac aggattagct gttattactt
    14101 tggaaaaagc attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc
    14161 aagggtttga aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac
    14221 gcaaagaaga actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt
    14281 cggttttaga agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac
    14341 tcagagaagt gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg
    14401 atatggcata tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg
    14461 caaatcaact agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga
    14521 tcgttgctag cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg
    14581 aacaaggtga cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata
    14641 tgacacgtac attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag
    14701 tcttagatgc tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat
    14761 tagatgcagt tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt
    14821 ctacaggaca tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag
    14881 aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa
    14941 tcggtggagt acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga
    15001 ctcgttcacc aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa
    15061 aatcagtgtt cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag
    15121 aacttttaag ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc
    15181 agctgatcgg cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg
    15241 atgtatctcg tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat
    15301 tttaaacgca aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat
    15361 tatcgggatt gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag
    15421 caatgtcact gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga
    15481 ggaagatggc ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa
    15541 agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga
    15601 tttatctgaa gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac
    15661 aatcgaagaa ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg
    15721 agattcggga aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa
    15781 aagaagatgc gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg
    15841 aatcagagtt tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag
    15901 atgaattgga taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct
    15961 caaaaatgga tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg
    16021 taccagcggc tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc
    16081 gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg
    16141 cgtaagtcct agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct
    16201 atggtagaat gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg
    16261 gaaaagaatt agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag
    16321 ataatggtat ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa
    16381 tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc
    16441 gttatcctga gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag
    16501 atcctagttt tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa
    16561 agatcctgtt agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag
    16621 gcagactcct tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa
    16681 tgtttgaagc ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca
    16741 atatcgtcgg taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg
    16801 ctcattctaa aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag
    16861 caataggacg tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag
    16921 atgtagggat gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag
    16981 tggagcctat tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca
    17041 cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg
    17101 cacaagaata tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg
    17161 atccttattt agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca
    17221 atgcccatca atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca
    17281 aaggagcatt ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg
    17341 ggcgtatttc attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc
    17401 ctgatggaat aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg
    17461 aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc
    17521 ttaccagtcc aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc
    17581 caattgtcca attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg
    17641 ttgtcaggaa tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc
    17701 gtgggggtgg ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga
    17761 tttatcaagc taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg
    17821 ctgatttagt agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc
    17881 ctgttttgtc agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt
    17941 ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat
    18001 ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc
    18061 gattatatca agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa
    18121 gccaccaatt ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg
    18181 cttatctaga aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc
    18241 gttttgctca agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag
    18301 gctacagcta tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag
    18361 gggatttgct gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag
    18421 acaaaggaga ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa
    18481 aaaattgtca tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt
    18541 aaacgaggaa tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc
    18601 ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc
    18661 tgagatgaga ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt
    18721 gtctttttta gatgagcata caacggatag aggactctat gatgcgatga gttattcagt
    18781 gaaagcaggc gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga
    18841 tgagcctatt gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta
    18901 ttccttgatc cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc
    18961 taccaatcat aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac
    19021 aggagcattc cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct
    19081 tcagcaatta gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat
    19141 cgagggcgaa tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac
    19201 agggcatttg attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga
    19261 aatcttattg ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga
    19321 tgatctactc gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga
    19381 acgtatggaa aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt
    19441 ggagattgag ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc
    19501 ttttgatggc gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa
    19561 agaatggaaa aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga
    19621 gaacaagcca aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt
    19681 gataaaccgg gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt
    19741 ccttatgttt ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct
    19801 gttgaaggaa aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct
    19861 ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg
    19921 aaaataagac aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa
    19981 cccgaagact ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca
    20041 ctaaaactga tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct
    20101 ttaatcaaac cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt
    20161 gaccctcaga cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga
    20221 tatgatgtgc tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc
    20281 ttggctcact tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca
    20341 gaagtggttt caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc
    20401 aagtgagaag tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa
    20461 gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac
    20521 tcgattgttg agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag
    20581 caaaggaata tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat
    20641 caaggtaccg gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga
    20701 tgtaaatact aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga
    20761 aaaatttgtt attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga
    20821 taaacgttac aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat
    20881 tacacgaact gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt
    20941 tgaggagcga atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa
    21001 ttgcggcttt cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa
    21061 tccattatta tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata
    21121 cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga
    21181 ttctctgaac tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga
    21241 cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta
    21301 gctaatctga gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag
    21361 gaactgctcc agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc
    21421 caacagaaga aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg
    21481 gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc
    21541 cagcaagaag aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa
    21601 gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga
    21661 gcactgacga ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg
    21721 caagggatcg cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct
    21781 tactatttgc tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta
    21841 gatgagagtc gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt
    21901 aaatacggcg aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta
    21961 gcttcttctg atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa
    22021 gagcttctac ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa
    22081 gaattggaaa aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt
    22141 gaggtacgtt tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc
    22201 gaattttaca ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct
    22261 ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt
    22321 gtgacaagta ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc
    22381 atcgcagata aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg
    22441 cctcaagttg ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga
    22501 cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt
    22561 atgctggctg gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa
    22621 ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc
    22681 tatattttat ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa
    22741 aatcagcatt gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt
    22801 ttcacgcatt gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc
    22861 catttccaca agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac
    22921 gatacaaata aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat
    22981 aggtaactta atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat
    23041 cgttaaagaa gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa
    23101 agtctgattc atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc
    23161 tgtttccacc aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt
    23221 attttgcgct agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac
    23281 gaggcttttt tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag
    23341 tttggcttcc aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag
    23401 tgtgacctgc caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg
    23461 aaaggtctgg ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag
    23521 cttctttttg ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct
    23581 aggacttgtt ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga
    23641 ttgattgaat tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc
    23701 tgacggaatt tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac
    23761 acaataccag atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa
    23821 tgtaaaatat ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag
    23881 ggaattggaa tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat
    23941 aaaatgaaac agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt
    24001 ttacgttcat gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg
    24061 ttttgtttgg ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa
    24121 aagcccctga tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata
    24181 cagggacaat ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta
    24241 ttgctgtttt ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga
    24301 cttggcgttt ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca
    24361 tactttctta cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg
    24421 cagcttgatt tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa
    24481 aaggtggtag aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg
    24541 ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg
    24601 ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc
    24661 tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg
    24721 ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa
    24781 tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct
    24841 ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca
    24901 cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg
    24961 attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact
    25021 atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg
    25081 tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta
    25141 aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt
    25201 tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag
    25261 aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt
    25321 cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg
    25381 atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct
    25441 accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg
    25501 ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg
    25561 aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa
    25621 gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt
    25681 tagaacaggc aatcgggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt
    25741 cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac
    25801 caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta
    25861 aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt
    25921 tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa
    25981 atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct
    26041 gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag
    26101 atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt
    26161 gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata
    26221 caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa
    26281 ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac
    26341 gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa
    26401 aagaaaaatc taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg
    26461 agtattggat tattcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat
    26521 gttgctggta cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta
    26581 aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc
    26641 tcttattcaa ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat
    26701 gttgaagaaa agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac
    26761 aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa
    26821 tttggtagct atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg
    26881 aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat
    26941 ggcgtctttt cttctcattt tattggatac gcagttccag acaaagatga gaatggctta
    27001 gttggtaaac taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag
    27061 attatttatc aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa
    27121 aaagctgtcg acggacaaga tatctacacg actttagaca gcagactaca aagttattta
    27181 gaaacactga tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg
    27241 atgaaagcaa aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa
    27301 acaatggaag gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt
    27361 tatgaaccag gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa
    27421 ttcaacgaga atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat
    27481 gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt
    27541 aacgtcggga tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa
    27601 aaactgggat ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta
    27661 cctacattaa atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg
    27721 aatttccaaa tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct
    27781 cgatatatca gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa
    27841 gttctagggc aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat
    27901 gttgtcgaaa gcgaaaacta cggtagtgct tatggtgtat atagtgtacc tggatacaat
    27961 gtatcggcaa aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga
    28021 gatacagcct acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc
    28081 ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca
    28141 aatccattga tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa
    28201 acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca
    28261 gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa
    28321 aaacaatcta ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact
    28381 ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg
    28441 gggaaaatcc tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa
    28501 cttgctccat atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa
    28561 gagaaatagg agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag
    28621 ttgtgcaatg acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca
    28681 aggtcaggcg attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac
    28741 aatgggcggc ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg
    28801 gcaaaaacag ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt
    28861 aatcggcttt cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc
    28921 gaagcagaaa ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc
    28981 tgaaggttat ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta
    29041 tggtattttc gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg
    29101 catcgatgga ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc
    29161 ttggcatcag catcagtatg atgtattaat catttgctta agtgttttag gaggtttact
    29221 gggtttcttc gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt
    29281 agcgttgggc ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct
    29341 tttgattggt ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt
    29401 taagcttaca ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg
    29461 cggatggtcg gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt
    29521 gatcacatta tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg
    29581 aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat
    29641 tacatgaatt gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg
    29701 aagcacaaga gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat
    29761 tgttggatga agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt
    29821 tagtagcaaa agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg
    29881 aagtagcgga atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa
    29941 cgatgacagg actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta
    30001 atattgggta tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga
    30061 tggaattgtc tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga
    30121 ttaccaatat ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag
    30181 ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc
    30241 agtcagaact acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa
    30301 aagaagtgtt ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa
    30361 aaataatgga aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag
    30421 ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc
    30481 attttttcca tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat
    30541 tttataatga ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg
    30601 aaacgtctaa ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat
    30661 taattccttc attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt
    30721 tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac
    30781 aagcagttcc tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag
    30841 cgaatgccag ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag
    30901 ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt
    30961 acaggcggag gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag
    31021 actcaagaac cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa
    31081 atcgtccctg atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa
    31141 atttccatgc ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa
    31201 aaaatcctca gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga
    31261 gcggtagtgt acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt
    31321 gtaccaggtg ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt
    31381 aaagatgcgg cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca
    31441 caagaagtag cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa
    31501 aagaaaacag tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta
    31561 tcagtttttt taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat
    31621 agatattatg aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag
    31681 ccttatatca aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct
    31741 ggtgcaactt cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc
    31801 ccatatgtta cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca
    31861 gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa
    31921 atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc
    31981 cctgatgctt caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg
    32041 tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa
    32101 acaatttgac tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa
    32161 accaagagcc aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag
    32221 aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt
    32281 ttgaagagat caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc
    32341 tgcccaattt aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca
    32401 ctgtattggg tataccgctg atttttttga tctattatgt ttctccttat agcaaattac
    32461 aagcagttgc agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa
    32521 aattgtcatt aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc
    32581 gccttaaaaa agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg
    32641 aattcgattt ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat
    32701 actacccagt gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa
    32761 atctccctat cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat
    32821 acaatcagtt atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag
    32881 caagcaataa aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata
    32941 tcaataatct tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa
    33001 aaggtgtgat cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa
    33061 cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa
    33121 acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa
    33181 acggatcatc tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta
    33241 caactccgtc aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt
    33301 tctaaaaacc agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat
    33361 attttaaaac ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc
    33421 attcatggca aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt
    33481 tgtcgtggct gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc
    33541 agaagggatt aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg
    33601 agcagtacga caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt
    33661 gccagcaaac atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc
    33721 aaaggaaatc accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat
    33781 acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga
    33841 gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt
    33901 tacaggacct aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat
    33961 cgtcaacgaa atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga
    34021 aaaagatttt ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat
    34081 gcatgataag caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa
    34141 agatatctct attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta
    34201 cggggatgca tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg
    34261 tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat
    34321 ggaacagatt ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc
    34381 aggtggcgtc gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca
    34441 agaaatcttc ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc
    34501 ggtatttaca aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca
    34561 attggcaaaa attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca
    34621 agaaacaaca aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc
    34681 ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat
    34741 ttttgattaa ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca
    34801 atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc
    34861 gaatgattga agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag
    34921 cattaaaaaa ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt
    34981 taggtgctgg ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct
    35041 tacgcgaagc tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa
    35101 ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg
    35161 ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag
    35221 aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt
    35281 tattagaagt tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg
    35341 ttttacgaca aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc
    35401 ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg
    35461 gtgtggctag tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat
    35521 tgcttgaaac atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg
    35581 atatgacatt gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg
    35641 atgtaaatat cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga
    35701 cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta
    35761 gacaagcaca aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac
    35821 caacttcttc agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg
    35881 ttcgtcctag agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat
    35941 tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc
    36001 gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag
    36061 caagaaattc aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta
    36121 gctgtaacaa aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg
    36181 aattgtgcag aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt
    36241 cctgaaataa agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta
    36301 aatgaaattg attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag
    36361 cggttagaga aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa
    36421 catggaattt caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa
    36481 atcaaggttg ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat
    36541 aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat
    36601 gcaccatgta gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa
    36661 ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa
    36721 aatgccgctt ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga
    36781 gtacgaatac caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa
    36841 tacaacacag aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa
    36901 aacggcgact tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt
    36961 aattgaacta catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac
    37021 ctctacacaa cctaaagcaa aattagaaca agtgaaaagt ccggaatggg atgaaatttt
    37081 acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa
    37141 acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc
    37201 tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa
    37261 cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg
    37321 gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta
    37381 agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta
    37441 aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg
    37501 gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa
    37561 aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg
    37621 ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt
    37681 tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa
    37741 aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt
    37801 tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa
    37861 acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca
    37921 agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc
    37981 ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc
    38041 tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc
    38101 ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc
    38161 tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac
    38221 gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta
    38281 tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt
    38341 ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat
    38401 ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga
    38461 tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga
    38521 tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg
    38581 attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat
    38641 gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag
    38701 ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt
    38761 tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt
    38821 tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat
    38881 ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc
    38941 aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat
    39001 tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc
    39061 tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc
    39121 ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt
    39181 agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat
    39241 caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg
    39301 ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt
    39361 tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt
    39421 ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg
    39481 tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga
    39541 tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg
    39601 agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt
    39661 gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc
    39721 agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt
    39781 gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa
    39841 agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca
    39901 agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc
    39961 attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt
    40021 ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt
    40081 attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa
    40141 cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga
    40201 tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg
    40261 tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga
    40321 aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat
    40381 cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt
    40441 ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa
    40501 aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag
    40561 cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt
    40621 tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga
    40681 ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt
    40741 cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt
    40801 catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag
    40861 tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga
    40921 agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt
    40981 tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc
    41041 cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt
    41101 tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta
    41161 atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt
    41221 agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga
    41281 tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt
    41341 tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata
    41401 cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt
    41461 ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa
    41521 cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt
    41581 gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat
    41641 ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag
    41701 gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac
    41761 ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga
    41821 acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca
    41881 atacattttt ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt
    41941 gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc
    42001 ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac
    42061 taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac
    42121 aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat
    42181 cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag
    42241 atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata
    42301 gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc
    42361 tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc
    42421 ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga
    42481 tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa
    42541 ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta
    42601 gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt
    42661 tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa
    42721 aggatatata tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc
    42781 tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg
    42841 atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc
    42901 gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact
    42961 aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt
    43021 tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt
    43081 ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt
    43141 tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac
    43201 tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt
    43261 atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta
    43321 tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg
    43381 tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg
    43441 cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc
    43501 ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg
    43561 gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt
    43621 tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata
    43681 aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat
    43741 taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac
    43801 atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac
    43861 atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg
    43921 taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat
    43981 agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc
    44041 aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac
    44101 atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag
    44161 atttgaagac ttttttcaat attactcata tggacaccat ttatattgct tctccgaata
    44221 gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa
    44281 agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca
    44341 atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg
    44401 attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt
    44461 cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag
    44521 gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa
    44581 tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat
    44641 gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt
    44701 cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg
    44761 caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa
    44821 cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc
    44881 cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga
    44941 accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg
    45001 aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga
    45061 ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa
    45121 taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg
    45181 gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc
    45241 tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt
    45301 gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga
    45361 aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa
    45421 aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc
    45481 tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc
    45541 ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac
    45601 gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga gacattactt
    45661 tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga
    45721 ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt
    45781 gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa
    45841 agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc
    45901 ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag
    45961 tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt
    46021 aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa
    46081 tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa
    46141 agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca
    46201 gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga
    46261 gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg
    46321 aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag
    46381 ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc
    46441 gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa
    46501 accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg
    46561 catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta
    46621 aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg
    46681 attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt
    46741 gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat
    46801 tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac
    46861 acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt
    46921 ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat
    46981 aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc
    47041 attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct
    47101 atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc
    47161 tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa
    47221 gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac
    47281 ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta
    47341 atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa
    47401 ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca
    47461 ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat
    47521 attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc
    47581 ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt
    47641 tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat
    47701 gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg
    47761 catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg
    47821 aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc
    47881 attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt
    47941 ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata
    48001 ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga
    48061 acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa
    48121 gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta
    48181 acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg
    48241 acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt
    48301 gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta
    48361 cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg
    48421 acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt
    48481 aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa
    48541 cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt
    48601 gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat
    48661 attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct
    48721 ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt
    48781 cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt
    48841 atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag
    48901 aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg
    48961 atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca
    49021 caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact
    49081 aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc
    49141 ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt
    49201 aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt
    49261 ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt
    49321 ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg
    49381 tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc
    49441 attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca
    49501 agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat
    49561 caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat
    49621 gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc
    49681 gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa
    49741 gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta
    49801 atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta
    49861 tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt
    49921 tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa
    49981 tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag
    50041 cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg
    50101 cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca
    50161 gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca
    50221 tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct
    50281 tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg
    50341 ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag
    50401 atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga
    50461 ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat catttgaatg
    50521 gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat
    50581 tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag
    50641 catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata
    50701 tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag
    50761 aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc
    50821 aagtattctg gttctttggt attcatggac caaatgtatt ggcaccagtg ctagagggaa
    50881 tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa
    50941 ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg
    51001 gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag
    51061 ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc
    51121 ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa
    51181 tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta
    51241 cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct
    51301 tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta
    51361 tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg
    51421 ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc
    51481 ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat
    51541 gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa
    51601 aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa
    51661 aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca
    51721 gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt
    51781 ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat
    51841 gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg
    51901 aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat
    51961 taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac
    52021 gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac
    52081 aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt
    52141 ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat
    52201 tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc
    52261 aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca
    52321 aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag
    52381 ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg
    52441 aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct
    52501 aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga
    52561 tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt
    52621 ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt
    52681 ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag
    52741 gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa
    52801 agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga
    52861 acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa
    52921 aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat
    52981 ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac
    53041 gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga
    53101 agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg
    53161 atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc
    53221 ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga
    53281 aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc
    53341 tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga
    53401 agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa
    53461 aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca
    53521 tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat
    53581 acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat
    53641 tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt
    53701 gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg
    53761 aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc
    53821 gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt
    53881 ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc tagtttatca
    53941 tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata
    54001 agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc
    54061 agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac
    54121 gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt gtgccaaaaa
    54181 tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga ttttttcttt
    54241 atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag tttctaaact
    54301 gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga
    54361 tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta attcttttaa
    54421 ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt ttaagttgaa
    54481 aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt tttgattgat
    54541 tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc tatttgaagt
    54601 ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat taatagtgat
    54661 gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag caaatcgcat
    54721 tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag aaaggttttc
    54781 ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat cttcttttgg
    54841 cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg gaattaatcc
    54901 ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa taacggtaga
    54961 ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc tatctaaatc
    55021 agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt cgtattttct
    55081 ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct caaagtaatc
    55141 tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat attctaattc
    55201 gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta tcttcgtgta
    55261 ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac gtttgactaa
    55321 tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata aagaagccca
    55381 ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta
    55441 tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga ggtgtttatt
    55501 atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt atttgttgat
    55561 tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt catattagaa
    55621 agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat attttgtagc
    55681 ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt tctaacagac
    55741 caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag ccagacatgc
    55801 tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac aattgaatct
    55861 caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc catgaagcgt
    55921 tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt ttattttgtg
    55981 cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt tggtccttgt
    56041 tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat gattcgttta
    56101 aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac ccaaaataaa
    56161 gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca actattaatt
    56221 caagatattc ttttttggtt agtttttcat tgctattatc catagttacc tcctattttg
    56281 tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg ttttattatt
    56341 gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac tttactatta
    56401 atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact aatttctcct
    56461 gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa
    56521 attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc atcgtactct
    56581 ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc ttcaaaagta
    56641 tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc tggagtaata
    56701 gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa aaattcttca
    56761 aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc agatgatgag
    56821 ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa acctacggta
    56881 gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt ttttttgata
    56941 aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt aaaaccaaat
    57001 ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa accaagcaat
    57061 tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa caactcttta
    57121 tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt atctaatata
    57181 gattgttgct tctctatata tttttttaaa ttttccattt cattccaata tttttttgta
    57241 aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact ttcgtttccg
    57301 cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa ttgtacggct
    57361 gatatatttt taaaggggat agttttttct cctttaatac cttgattaac taaatttata
    57421 aatccttttc taataattga aatagaatca tcagttactt taatcaaggt ttttcctgga
    57481 gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata tgattttttt
    57541 tgtagaagct catatatgag gaaagagccc gtgttgcagc acgggctttt ttaatgtttt
    57601 gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa
    57661 tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga
    57721 atagaataat actccataaa tttttcgaga ttaaactgag atttatcagt cagttcgttc
    57781 tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt
    57841 gagtgaaaag ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa
    57901 tgtgcaagtt catgagataa atgaaaggct tctgcagttt caccgtatag attttcattc
    57961 aaaaaaatga ttctgggttt tggatagtag aaaccaggct ctttcatctc catataaact
    58021 actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca aaccatcact
    58081 ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct
    58141 tagatatttc ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt
    58201 taggggtttc tctttcttct ctacctagaa ggtagtctac agagacatcg aaatagtcag
    58261 caatccgact taattcatct gaattaggag tattattttt ccactttgct agataaccgt
    58321 tagaatatcc aaaattaatt tctaactgcc gtatagatat ctttttcctc ttcgccaagt
    58381 cttttattat ttcataggta ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa
    58441 gtttagaaaa atacgcagaa ataatttgac taattctgag taatgagcta tactatatct
    58501 tgtaaacaga tttaacaact aaaaagacaa caaaaaacac tattgatatg taaatgcaga
    58561 ccgccaagaa agctttaaaa tcaatgtttt tatgtcttat ttaattatgc tcttagtata
    58621 gagtattact cagatactgt caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa
    58681 tataaaagaa aggaatgaga aatatgaaca caccgcaaat ttttaatttc gaacaaaacg
    58741 aagttcggac aattttagta aatgatgaac catattttgt aggcaaagac gttgcgagtg
    58801 ttttgggtta ttcaaacact aaagatgctt tgtcgcggca tgtagatttg gaagataaga
    58861 tggggtcgcg aatcacgacc tcaggtcaat caagagagat gacaatcatc aacgaatctg
    58921 gtttgtacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg
    58981 taacaagtga agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag
    59041 tagaagaagc tttgcttaat ccagatacaa tcattcaatt agcaactcaa ctaaaagaag
    59101 aaagaactgg aagattaatc gcagaacaaa agattgcaga gtacgaacct aaaatctcct
    59161 atttagatag catattatct tctacagatt cagtaacaat tagtcagatt gcagcagatt
    59221 atgggatgtc tccacaacag atgaataaat tacttcataa actaggtatt cagaaaaaag
    59281 tcggtaacca atggttattg tgcaaaaaac acatgaacca aggatacaca aaatctcata
    59341 caactgagat cccgaaagcc gatggtggca ctaaaattgt aatgaatacc aaatggacac
    59401 agaaagggcg tctatttatc tacgaattac taaaaaaaga aggatattac cctcaaatgg
    59461 atttagagga aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca
    59521 acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat
    59581 gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa
    59641 gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtaaag
    59701 ggaggaacag ctaatgcaat atctagaagc aaaaatccca attccagaag gctatgtaat
    59761 tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta gatggatgac
    59821 gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt ctagagtttt
    59881 aaagaaccca agatatagaa atattatcga tatcgagaaa aacaaaaatg gatttgtcta
    59941 ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac ttgaattttt
    60001 agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt aatagagcgc
    60061 tagtgttcag cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt
    60121 tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg
    60181 agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact
    60241 agaatgtata ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt
    60301 gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt
    60361 gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa
    60421 gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca
    60481 tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt
    60541 aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa
    60601 atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct
    60661 agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa tataaaactt
    60721 attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa caccaccagt
    60781 ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag taattttgaa
    60841 aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga aaaaacatca
    60901 acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg gacagatgtt
    60961 tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct tcattagtag
    61021 agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct acttcaaaca
    61081 aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata ctgttcctca
    61141 aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta aaaaagatgt
    61201 ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa
    61261 aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt atgaagaaat
    61321 cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag
    61381 atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt
    61441 gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga gactttttgg
    61501 tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct aattgctggt
    61561 ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc
    61621 aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag
    61681 gagattttag catatggaaa aagaactttc cactctagat caatatttga ttgatcctga
    61741 ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa atcttttgac
    61801 gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta tttgggatca
    61861 tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt acgtcatcga
    61921 agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg atatctctta
    61981 ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaatca
    62041 aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa
    62101 aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc
    62161 cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct ggaactcatc
    62221 aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg acttatgtat
    62281 ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt atttcctgtt ttagattatc
    62341 gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct
    62401 ttgtgaaagc attggctcta cacggactgg gattatatgt atttcaaggg gaagatattc
    62461 caacaccacc tagaatcgat acaaagaaat taaacatgct agagacgatt ctagaagctt
    62521 tcaatgagca gatgggtaaa gatatgacca aaaccttaat cgaatatgtt aatgagcaga
    62581 cagataaatt agggctctta gctgataacg ttgaaactat tgaacagtta agctatgagc
    62641 aatgtgcctt gatggagcga gcaatagcag ctaagagaaa agaattagat aagaagtgat
    62701 atgagtgttt aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa
    62761 agacataagc gcaaccatca atgaggaagt gaacattgat cgactaaaga cgatgtatga
    62821 cggctacgat ggtgatcgag tcattgaaat tcgttttatt gatcctagac gtttcaccgt
    62881 acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata caggcatgcc
    62941 aacggacttc tggaaggaat tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat
    63001 aagcctgaaa gacgaatcga atacaactgt gagtgatgcc aatgtcttag caaatatcat
    63061 cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttacctgc
    63121 gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa
    63181 aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt
    63241 tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag agaagcacaa
    63301 aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat taaaccagaa
    63361 aacaatcaaa aagttaaata ttggagggta aaaatgacag aacatcgaag ttattatgcg
    63421 attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa gttgttatac
    63481 ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt
    63541 gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga tttgattaag
    63601 tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat aatcaatagg
    63661 tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat tgacgaaaaa
    63721 gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga atatataaga
    63781 gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
    63841 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt ccctaatgat
    63901 taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa gacttataaa
    63961 aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc taagaacgca
    64021 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc aatcgaccaa
    64081 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
    64141 taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata
    64201 aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa
    64261 ttgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
    64321 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
    64381 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
    64441 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
    64501 gaagacaagt ggctcaacac gttccatggc ggacgtgaga gcgcacttga tcaagtactc
    64561 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
    64621 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
    64681 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
    64741 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
    64801 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
    64861 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
    64921 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
    64981 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
    65041 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
    65101 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
    65161 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
    65221 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
    65281 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
    65341 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
    65401 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
    65461 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
    65521 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg
    65581 gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga
    65641 agagtatttg aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat
    65701 atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg
    65761 gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc
    65821 atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga
    65881 ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa
    65941 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
    66001 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat
    66061 gcaatccaca ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt
    66121 agtcagcgtg cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc
    66181 aatagattat tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt
    66241 taacacagag ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag
    66301 cgagttattg gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt
    66361 aaagcgatag gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt
    66421 acgttaatga aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt
    66481 tggcttgaga aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa
    66541 tctaaagatg gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg
    66601 caagagcaag gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg
    66661 agcctataca acggctacga ggttgagaaa gagccgttat gggcaataaa gaatgccgat
    66721 ggaaactatc ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa
    66781 tgcaatccat ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg
    66841 ttggtgaatg gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg
    66901 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
    66961 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
    67021 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
    67081 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
    67141 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
    67201 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
    67261 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
    67321 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
    67381 atgccgaagg caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat
    67441 atagaaacgg agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
    67501 cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt
    67561 gaagtgggtt attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt
    67621 gcagaagcat ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg
    67681 gaatgatggc ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga
    67741 aacaggatcg acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct
    67801 tagatagaca gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt
    67861 catcggttgc tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt
    67921 cgtctttcaa ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat
    67981 cagtagaagg cactccagaa gaaatacaag aattgctcca agctattggt agtagcaagg
    68041 agcaaaaggt aagtgtttct aattcagata ttgacggtat ttgcaattgt ttaaacagag
    68101 aaaaaatatc taatccttac taagagaaaa tatgttcgtt aatgaatttc caattttcct
    68161 ctgtagctct accttgataa tttttttgca gttcacagat gaataaggaa tcgttattat
    68221 caagtactgt atttaatttt tcacacattt gatttggatt caaattagtt ttaactaacc
    68281 aaaaagattt ttgtagcttt atatatgccc caaattcttt tattatttca aatactttat
    68341 catacttttg ccctgggtta ttaagatcat aactaatcat gtagggttta tacatgttgt
    68401 cttgcacatc cttgttttat atttcagcgg accactcgct gataaataaa attatacgct
    68461 tagtatttat tttcacaata ttaatttatc gtaataactt ttgagtaaat agaacaaaaa
    68521 acctgcacta gtttccgcta gtacaggcag tgactatatc ggttgataat ctagcatata
    68581 ttaaaaataa ttgcaagaaa agaaatttat agtggtttat accaaattat caaagcaatg
    68641 ttattttgtt gctgtctatt gttttttaat tattcacacg ataactaaag gtgggtgaag
    68701 agaaatgatt ccattaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg
    68761 atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat agtgtcactg
    68821 tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca accatgcaag
    68881 gttcgattcc ttgccagcga cattaaatgc ctatgacggt tacgactacc gaaaaaagat
    68941 cgttaagaag ctatacggtg ctacgtacgg caatgtagta agtgtgctat ctgtacacca
    69001 ccaagcttcg gtcactgtgg cggaagtaga agacgcagcg gtaaatggcg agtagcctcg
    69061 tgagagcctg gtaagttctc gtgagtggtg caatcccact ccagcgactt taagcaaccg
    69121 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
    69181 caagttacta ttacatatta gatcactctt tgagtggtct ttttattttt gcacaaagga
    69241 ggaaacaaca atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgttca
    69301 taaaggtaat ggcgaaaaag tatatgagta tagaagacca ataaagagcg atacatataa
    69361 acgaaaggaa aacaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
    69421 aagattgcga aaggagacgg aacatgaccg aggaattcta tagatggcta ttacagttga
    69481 caagagaaga tcgtttggtt aagttctatc agtctcctaa atggcgcagg cttagagaga
    69541 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
    69601 gagtagagaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
    69661 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
    69721 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
    69781 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
    69841 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtaggt
    69901 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
    69961 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
    70021 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
    70081 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
    70141 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
    70201 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
    70261 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
    70321 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
    70381 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
    70441 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
    70501 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
    70561 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
    70621 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
    70681 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
    70741 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
    70801 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
    70861 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
    70921 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
    70981 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
    71041 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
    71101 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
    71161 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
    71221 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
    71281 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
    71341 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
    71401 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
    71461 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
    71521 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
    71581 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
    71641 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
    71701 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
    71761 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
    71821 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
    71881 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
    71941 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
    72001 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
    72061 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
    72121 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
    72181 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag
    72241 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
    72301 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
    72361 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
    72421 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
    72481 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
    72541 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
    72601 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
    72661 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
    72721 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
    72781 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
    72841 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
    72901 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
    72961 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
    73021 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
    73081 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
    73141 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
    73201 atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg
    73261 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
    73321 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
    73381 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
    73441 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
    73501 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
    73561 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
    73621 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    73681 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    73741 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    73801 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    73861 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    73921 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    73981 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
    74041 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    74101 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    74161 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    74221 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    74281 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    74341 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    74401 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
    74461 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    74521 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    74581 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    74641 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    74701 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    74761 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    74821 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    74881 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    74941 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    75001 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    75061 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    75121 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    75181 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    75241 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    75301 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    75361 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    75421 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    75481 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    75541 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    75601 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    75661 caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa ttattggaaa aaggtttatc
    75721 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata caaatctgga
    75781 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    75841 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    75901 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    75961 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    76021 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    76081 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    76141 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    76201 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    76261 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    76321 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    76381 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    76441 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    76501 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    76561 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    76621 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    76681 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    76741 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    76801 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    76861 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    76921 gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat tcaaaatcgt
    76981 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    77041 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    77101 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    77161 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    77221 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    77281 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    77341 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    77401 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    77461 tctgattcgt caattcgata aattaaataa atagggggaa caacaatggc taaaacaaaa
    77521 actgtagtaa caacgttcga taacgtaagt atcaaacgaa ttgcttttaa ttttaagaac
    77581 gcaggaaatg caatcgcaac gaattgtaac ggacaattag atggcgaaac agaaatgcaa
    77641 acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc aatcaatatg
    77701 acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa
    77761 caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt aggcgaagat
    77821 ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt agttggtatg
    77881 ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta
    77941 gctgcgttag aactagaaac aaaagttatg caagatgaat ttggtaaatt ttatcatgaa
    78001 gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct
    78061 gatgtgatta aaaaaacttc agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa
    78121 cggaggtagc gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt
    78181 gaatttagaa cctaaactga atatcaagaa attattgatg atcaatagag attttaacac
    78241 agacgagttt gcaaaaatga ctgtgggaaa aggatccatg gatatttctg ttattcaagg
    78301 tgcaaaggct gtgtatattg cttaccgcca agcgaacatg actgattata tttcattcga
    78361 tgaatttatc gataaatggg attttgatat ggctactgcc agctatattt atcaattgat
    78421 gatgttcaaa caagcacgcg atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa
    78481 aaagcttcaa aagtaaaaat tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg
    78541 atgttgaccg acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat
    78601 gacattttgc aaatggctta caacaagagt gcttatgaag gatggaaaaa ctatgcaata
    78661 aaccaatccc agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc
    78721 agaagtaact ttcatagcta acgatgacgg attgaaatct acgttaaaag aaatcagtgc
    78781 tgaattaact aaaaatagag cagaattaaa actagaacaa gctcaattac aacagactgg
    78841 ttctgaatca gacaagttag gaagtaaatt atcttcttta gaaaagcagt atgaattaca
    78901 aagtcaaaaa gttgaagtaa ctagccaacg tttagccaat gcgaaaaaat attatggaga
    78961 aaattccacc gaagttaaga aacttgaaag agaactgatt aatcaacaaa cagcgcaaca
    79021 acgtttgtca aacgaaattg ataaaacgag taatgcacta gctcaagcaa aaggcgaaat
    79081 acagacgtac gagtctacaa tgcaacagtt ggatagcgaa caaaaaaatg ttcaagctag
    79141 tgcttctctg attgaatccg aatacaaaaa atggcaagca actgctggtc aatcagcttc
    79201 tgaagccgag aaattagcga aagcccaaga atatgtttct caacaatctg aaaatgcgga
    79261 gaaaacgata gatatcctaa gacgacagtt agaagctaca cagtctgaat ttggcgctac
    79321 atccacagaa gcaatgcaga tggaagcgaa gcttaatgat gctgaacgtg aatttgaaga
    79381 gttaggacaa gctgctaaaa atgtagatac aactaacttg gacgatatcg gaagcaaaat
    79441 agatatgaat aatttaatgg aagcttctga cgttttaagc gacattggcg ataagcttac
    79501 agaattaggg aaacaagcag tggactctgc taacagtgta ggtagttccc agagtaagat
    79561 acaagctaat tttggtttgt ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga
    79621 catttattat aaaggttttg gagaatcgtt agatcagtcc acagatgcat tgattttggt
    79681 aaagcgtaat ttaggagatt taaataatca agatttacaa aatatcacgg aacaagctat
    79741 ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg taaatggctt
    79801 aatggtcaat ttcggcttga gtgctcaaga tgcaatggat ttaatggttt cgggtactca
    79861 aaacggttta gataaaacgc acgaattagg cgacaatatg gcagaatata gccaattatg
    79921 gagtcaaatg ggatattcag ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc
    79981 gggtgcttat aaccttgata aagtcaatga cttagttaag gaaatgggaa tatcgttaac
    80041 agatggtcga tttgagcaaa acatggatat gtttagtgaa agtactagaa aagcttttga
    80101 agagtggaaa aatggcggag gaacacaaaa agacgttatt aattccatga ttcaagattt
    80161 tagcaatatg gatggtcaat acgaccaatt aaataaagct tcgacaattt ggtctgcgct
    80221 tggcgaagat aatgcgatga aagttgtcca atctttaact gatgttaacc atacatttga
    80281 tgatgttagt ggatctgcac aaaaaatgaa tgaagattct actactccgt tgcaagagtt
    80341 gaacgggaaa atagctgaat taaaggattc attagctcct ataggcaaca caatcataga
    80401 tgcactcgaa ccagtaattg attttctagg aaagatggct gatgcgttta ataatcttcc
    80461 acaaccagta caggatttca ttgttgctat aggtggtctt acagcagcat ttggcatact
    80521 gttaccggca atactagccg tgtcatattt atttggtccg atgatgctta taattggagg
    80581 aattatagct gttatagcag gcgttattgt ggcaattaag aactggggtg caattactga
    80641 ctggtttagt ggtttatgga aaaaatttac tgattggttg ggtgatactt gggaaagtat
    80701 aaaagacgga gcctcatcag tttgggatgg ggttaaagaa acctggtctg gatttgtaga
    80761 ttgggttcaa gatatttggc aaggagtttc tgattggttt ggagagctat ggagcggatt
    80821 agttgaagga gcttccaaca tctggcaagg agtccaagaa acttggcaaa cattcgttga
    80881 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    80941 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    81001 tattatcgtg ggtatttttg atgtagctgg acagttattg ggcgcagctt ggcaagctgt
    81061 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    81121 tatcgcttgg aatgggattg taactacatt ccatactata tttgatccag tagtggcatg
    81181 gtggaatggt atatggacag ctattagtac tacggcttca aatatttgga attcaattag
    81241 tgcaacagct tctagtattt ggaacagtat caagaataca atcactagct tggtacaagc
    81301 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    81361 gaattcaatc agctctacag catcaaatat ctggaatagt gtgactagta gtataagcaa
    81421 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    81481 tagcggaatt tggaacggta tcaaaaatac tgctttgaat ctttggaatg gaattacaag
    81541 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    81601 aacaggtatt gtttccgaca tattcaataa tgttaaaagt acaattgcta acatatggga
    81661 aggcatcaaa aagactgtta gcgctccaat tgattggatt agagacaaaa tcagtggcat
    81721 ttttgataat ttgaatattt ctataccaca tattccgtta ccggaattta tcatggaagg
    81781 cagctttaac ccgctaaaag gtcaaatccc ccatttgcgt gtaaaatggc atgctaaagg
    81841 aggtatcttt actaaaccaa ctttactagg tggaatgaac ggtgtcggtg aagcaggacc
    81901 tgaagcagtt ttacctttga aaagatctgt tttgcaagaa attggtgatc gtatcttgag
    81961 tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa
    82021 tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat
    82081 tgatagcatt acaaaaattc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    82141 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    82201 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    82261 ccttctccaa aaagacagag agttgaaact tctgttttag gtaggttagg tggcttagta
    82321 caagattact cgtttgaaga tatgtcgttt acattgcact ataactattt agaggatgtg
    82381 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    82441 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    82501 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    82561 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    82621 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    82681 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    82741 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    82801 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    82861 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    82921 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    82981 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    83041 agcaataaac taaacggagc cgagatattt cagggtactt attcaataaa cggaactaat
    83101 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    83161 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    83221 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    83281 gaaaagatca ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa accagctatc
    83341 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag
    83401 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggt
    83461 gagtttctaa aagataataa cacattacgt catgaaaaaa gtctaggtac ggatcataaa
    83521 atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt
    83581 gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact
    83641 ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    83701 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    83761 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    83821 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    83881 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    83941 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    84001 aacttcttag aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa
    84061 ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat
    84121 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    84181 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    84241 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta
    84301 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    84361 ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg
    84421 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    84481 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    84541 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    84601 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    84661 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    84721 actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg
    84781 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    84841 gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac
    84901 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacaat
    84961 ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat
    85021 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    85081 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    85141 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    85201 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    85261 atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    85321 gtgataaagg gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga
    85381 gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta gataatgttg
    85441 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    85501 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    85561 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    85621 atgtttcgca attagaagag ttgattcgaa agtacaacga aacttttgat gcttttatgg
    85681 ctgaaatcaa aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag
    85741 ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag ttaggtaact
    85801 tacaagtgat gtacagtaac agcatcgact tcggggacta tgattattcg gagaacccga
    85861 atttaatgcc atatattaca gaaccttggg ttggaccttt gttaggtaat ggacatacag
    85921 ttaaagatag tgttaaaagg gttataactc atactaaaac tagaacagct aattccggcg
    85981 acatattatc gcttggctta ggtattccgt gtacagcgga agctaataat aggtatctta
    86041 taaccacttt acgaccatct acgacataca cattaagcgt tactatgagt gtcggttcag
    86101 attggactgg agagaccaat actatagggg ttaggcttag atatttaaat gaacaaggtg
    86161 gtatagaact gcccattaat gcattaatac cagcaaatgt agaacgcgat aaaatggtca
    86221 ctcatacatt tactggtata acaaaagata atgtaactag cataacaaac tgttatgttg
    86281 aaatattctc acttaatagt gaatataaag gaacggttag tgtcagctat gacgttaagt
    86341 taaaagcaca ctaccctaac ttactagatg gcccttactg gttaggtaaa gttcctttag
    86401 gcgagaatat tgctgaccct acagttgtct ttccacacaa aactagtgaa tatatggttt
    86461 atggtagacg caatacagaa aactatatcg ctgaccaaac atataccata tcaatgaaag
    86521 caactaaact aacagttcaa tcgtttgccg tgtatattgc tgctggccgc gttaaagtag
    86581 gagacatgaa gcctactgag gggttagcta atacatggga gttaactttt actgtaacta
    86641 agcaacatat agatagtggg gtaactaatt atctggaaat ataccaatac ccgtcagcta
    86701 caaaaggagc tgttcaaatt gagtggctca aactagaaaa aggcaatact cgaaccccga
    86761 atattagtga gtataaatat cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt
    86821 atgtttggga tctagcacca gaatatgtcg aagataattt ggccacagat attaaaattt
    86881 ctgaaattac tggtaaagca aacaattata ccgatgggaa agtatcggag attaattcgc
    86941 agttgactgc ttcaattaat gaagtagaca ccacagctaa ggatgctcaa acaaaagcga
    87001 atgctaatgc gactgctata gatgaattag acaataagat cgatgaacgc attaatgata
    87061 cagctactac cacattaaca gttacaaacg ggaataccgg atcagcaaag ctttatcgtg
    87121 aaggaaaaac agtttctata tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg
    87181 attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt gagcaactgg
    87241 ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt gcagatggag
    87301 ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca attacttaca
    87361 cgatttagaa aagcgtgaat cgatatgaag gcagcatata gaccaattga accttacgga
    87421 ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac agaagtcgaa
    87481 ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa atgaaaaaca
    87541 tttggaaata tggacgtact ggcggagagt acgcaggaaa agtattggac gacatgcttg
    87601 tatccgttcc ttacacagat cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt
    87661 taacaatcgc tgatcaaatg tttgatccta aattgaatca atggattatt ttagcgaacg
    87721 cactagatca caacgattta aacaatctca aagcaatgta tgagtcgtta gaaaatgaga
    87781 acggcgattt aaaacagatc aatgccaaac tcatgctaag cgatgtagca attaaacagg
    87841 aaaatactgc attgaaagaa aaagcggata gtttagcaca aatcaattca aaaatgatgc
    87901 ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta aatccagctt
    87961 caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta tgattggggc
    88021 tgtttcacta acgaacaagt aatggttttc gttccgttgt gcattactga agaaaaagca
    88081 gataaaatca ttagcaaaga agagagcgca tcttaattga tgtgcttttt attttgattc
    88141 aaggagttgt cacatgatta atttagggga atggggagcg atagcaggat caataaccgc
    88201 tatcgtttct ttgattttat tagtaataaa accaattact gcatctttct cgaagattac
    88261 tgagactctt tcaaaagtaa gtcacaattt agatttgctg actaaagatt tagaatcgag
    88321 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
    88381 acatgcagaa aaattggtag aacacacgca acaaattaaa actttattta gggagagaag
    88441 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
    88501 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
    88561 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
    88621 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta
    88681 ctattactgc gatgagcctg ttaacggctc tttttttatt acccattaat acgtttgctt
    88741 atactattaa tgacgagtat aatttagcgc cgaatcaagg agactccaga ttagcaattc
    88801 ctaacaaaat tattttgcac gaaactggaa tagatgcacc agcaagaaac gtagccgcca
    88861 acatgaaaaa taattataac ggaagcaatc cttatactac agatgttatt ggtgacggtg
    88921 ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg gggagctggt aacgctaatc
    88981 cttatgcgcc tgtacagatt gaattacagc gcacatatga taaagcattg tttgaaaaaa
    89041 actatcgagc ttatattgaa tatacaagag atagtgcaaa aaaatatgga attccattga
    89101 ctcttgatca aggaacttct ttatttacaa aaggaatcat ttctcatttg tgggtgacaa
    89161 attatgtttg ggggaaccac acagatccat atggttactt atcgcaaatg ggagttagca
    89221 aagaaaagct tgcttatgat ttagctcatg gatttaccga tgaaaatcca acaacttctg
    89281 aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc taatcctacg ctgacagatg
    89341 gaacaaatca cgcccacatt gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg
    89401 ctggatggca cattgctaac tataaatacg agtatatttt cattatggac tacaatactg
    89461 ggaaagaatt agctcgagta agagctgatg gaatttatag accagatgta aaccaagctt
    89521 ataatacttc tggaaatgtt ggttatcatg tatctttcaa tatgcgtaat tttcctagta
    89581 agaaagtcta tgtaatgatg cgggcaacga atgatccaga gggaaacact aaaggcggtg
    89641 cgcaagattt ccatgacaaa cgttggtatt taaatattcc taaacgataa aaatagctcc
    89701 tcgttgagga gcagtacata actatattga caactataaa aattattcga taaaatagtg
    89761 atgttatcgc atatcttcac tatcacccat
//
LOCUS       DACETN010000002        84706 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DACETN010000002 DACETN010000000
VERSION     DACETN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.002"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(606..962)
                     /locus_tag="IXF98_000111"
     CDS             complement(606..962)
                     /locus_tag="IXF98_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1554455.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IXF98_000112"
     CDS             complement(976..1386)
                     /locus_tag="IXF98_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1554456.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="IXF98_000113"
     CDS             complement(1732..3918)
                     /locus_tag="IXF98_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1554457.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="IXF98_000114"
     CDS             4136..4819
                     /locus_tag="IXF98_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1554458.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="IXF98_000115"
     CDS             complement(4900..5619)
                     /locus_tag="IXF98_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1554459.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="IXF98_000116"
     CDS             complement(5931..6137)
                     /locus_tag="IXF98_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554460.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="IXF98_000117"
     CDS             6382..6984
                     /locus_tag="IXF98_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1554461.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8406
                     /locus_tag="IXF98_000118"
                     /pseudo
     CDS             7125..8406
                     /locus_tag="IXF98_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            8419..9147
                     /locus_tag="IXF98_000119"
     CDS             8419..9147
                     /locus_tag="IXF98_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1554462.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9216..10739)
                     /locus_tag="IXF98_000120"
     CDS             complement(9216..10739)
                     /locus_tag="IXF98_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1554463.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10991..12172
                     /locus_tag="IXF98_000121"
     CDS             10991..12172
                     /locus_tag="IXF98_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1554464.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12232..13860)
                     /gene="treC"
                     /locus_tag="IXF98_000122"
     CDS             complement(12232..13860)
                     /gene="treC"
                     /locus_tag="IXF98_000122"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1554465.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14311..17022)
                     /locus_tag="IXF98_000123"
     CDS             complement(14311..17022)
                     /locus_tag="IXF98_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1554466.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17032..17733)
                     /locus_tag="IXF98_000124"
     CDS             complement(17032..17733)
                     /locus_tag="IXF98_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554467.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17919..18518
                     /locus_tag="IXF98_000125"
     CDS             17919..18518
                     /locus_tag="IXF98_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1554468.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18599..19612
                     /locus_tag="IXF98_000126"
     CDS             18599..19612
                     /locus_tag="IXF98_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1554469.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19720..20637
                     /gene="rbsK"
                     /locus_tag="IXF98_000127"
     CDS             19720..20637
                     /gene="rbsK"
                     /locus_tag="IXF98_000127"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1554470.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20665..21060
                     /gene="rbsD"
                     /locus_tag="IXF98_000128"
     CDS             20665..21060
                     /gene="rbsD"
                     /locus_tag="IXF98_000128"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1554471.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21060..22433
                     /locus_tag="IXF98_000129"
     CDS             21060..22433
                     /locus_tag="IXF98_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554472.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22790..24934
                     /locus_tag="IXF98_000130"
     CDS             22790..24934
                     /locus_tag="IXF98_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1554473.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24986..26027)
                     /locus_tag="IXF98_000131"
                     /pseudo
     CDS             complement(24986..26027)
                     /locus_tag="IXF98_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(26304..28106)
                     /locus_tag="IXF98_000132"
     CDS             complement(26304..28106)
                     /locus_tag="IXF98_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554474.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28120..28872)
                     /locus_tag="IXF98_000133"
     CDS             complement(28120..28872)
                     /locus_tag="IXF98_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554475.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28881..29243)
                     /locus_tag="IXF98_000134"
     CDS             complement(28881..29243)
                     /locus_tag="IXF98_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1554476.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29419..30291)
                     /locus_tag="IXF98_000135"
     CDS             complement(29419..30291)
                     /locus_tag="IXF98_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1554477.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30342..30830)
                     /locus_tag="IXF98_000136"
     CDS             complement(30342..30830)
                     /locus_tag="IXF98_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1554478.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30968..31759)
                     /locus_tag="IXF98_000137"
     CDS             complement(30968..31759)
                     /locus_tag="IXF98_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1554479.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31814..32422)
                     /locus_tag="IXF98_000138"
     CDS             complement(31814..32422)
                     /locus_tag="IXF98_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1554480.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32415..32732)
                     /locus_tag="IXF98_000139"
     CDS             complement(32415..32732)
                     /locus_tag="IXF98_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1554481.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32888..33616)
                     /locus_tag="IXF98_000140"
     CDS             complement(32888..33616)
                     /locus_tag="IXF98_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1554482.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33775..34656
                     /locus_tag="IXF98_000141"
     CDS             33775..34656
                     /locus_tag="IXF98_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1554483.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34893..35936)
                     /locus_tag="IXF98_000142"
     CDS             complement(34893..35936)
                     /locus_tag="IXF98_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1554484.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35939..36607)
                     /locus_tag="IXF98_000143"
     CDS             complement(35939..36607)
                     /locus_tag="IXF98_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1554485.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36749..39085
                     /locus_tag="IXF98_000144"
     CDS             36749..39085
                     /locus_tag="IXF98_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1554486.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39200..39511)
                     /locus_tag="IXF98_000145"
     CDS             complement(39200..39511)
                     /locus_tag="IXF98_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554487.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39692..41167
                     /locus_tag="IXF98_000146"
     CDS             39692..41167
                     /locus_tag="IXF98_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1554488.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41214..41798
                     /locus_tag="IXF98_000147"
     CDS             41214..41798
                     /locus_tag="IXF98_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1554489.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41800..42219
                     /locus_tag="IXF98_000148"
     CDS             41800..42219
                     /locus_tag="IXF98_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1554490.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42500..42784)
                     /locus_tag="IXF98_000149"
     CDS             complement(42500..42784)
                     /locus_tag="IXF98_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1554491.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43016..43726)
                     /locus_tag="IXF98_000150"
     CDS             complement(43016..43726)
                     /locus_tag="IXF98_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1554492.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43723..45195)
                     /locus_tag="IXF98_000151"
     CDS             complement(43723..45195)
                     /locus_tag="IXF98_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1554493.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45333..45806
                     /locus_tag="IXF98_000152"
     CDS             45333..45806
                     /locus_tag="IXF98_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1554494.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45959..46510)
                     /locus_tag="IXF98_000153"
     CDS             complement(45959..46510)
                     /locus_tag="IXF98_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1554495.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46615..47145
                     /locus_tag="IXF98_000154"
     CDS             46615..47145
                     /locus_tag="IXF98_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1554496.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47628..48071)
                     /locus_tag="IXF98_000155"
     CDS             complement(47628..48071)
                     /locus_tag="IXF98_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554497.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48087..49247)
                     /locus_tag="IXF98_000156"
     CDS             complement(48087..49247)
                     /locus_tag="IXF98_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1554498.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49754..51454
                     /locus_tag="IXF98_000157"
     CDS             49754..51454
                     /locus_tag="IXF98_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554499.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51556..52389)
                     /locus_tag="IXF98_000158"
     CDS             complement(51556..52389)
                     /locus_tag="IXF98_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1554500.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52693..54858)
                     /locus_tag="IXF98_000159"
     CDS             complement(52693..54858)
                     /locus_tag="IXF98_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554501.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55118..57412
                     /locus_tag="IXF98_000160"
     CDS             55118..57412
                     /locus_tag="IXF98_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1554502.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57405..58070
                     /gene="pgmB"
                     /locus_tag="IXF98_000161"
     CDS             57405..58070
                     /gene="pgmB"
                     /locus_tag="IXF98_000161"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1554503.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58063..59070
                     /locus_tag="IXF98_000162"
     CDS             58063..59070
                     /locus_tag="IXF98_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1554504.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59092..60111
                     /locus_tag="IXF98_000163"
     CDS             59092..60111
                     /locus_tag="IXF98_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1554505.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="IXF98_000164"
     CDS             complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="IXF98_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1554506.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61868..63289)
                     /locus_tag="IXF98_000165"
     CDS             complement(61868..63289)
                     /locus_tag="IXF98_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1554507.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="IXF98_000166"
     CDS             complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="IXF98_000166"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1554508.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65700..66956)
                     /locus_tag="IXF98_000167"
     CDS             complement(65700..66956)
                     /locus_tag="IXF98_000167"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1554509.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67536..68837
                     /locus_tag="IXF98_000168"
     CDS             67536..68837
                     /locus_tag="IXF98_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1554510.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68906..69505)
                     /locus_tag="IXF98_000169"
     CDS             complement(68906..69505)
                     /locus_tag="IXF98_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1554511.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69759..70640
                     /locus_tag="IXF98_000170"
     CDS             69759..70640
                     /locus_tag="IXF98_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554512.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70653..72262
                     /locus_tag="IXF98_000171"
                     /pseudo
     CDS             70653..72262
                     /locus_tag="IXF98_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(72339..72998)
                     /locus_tag="IXF98_000172"
     CDS             complement(72339..72998)
                     /locus_tag="IXF98_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1554513.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73281..73946)
                     /locus_tag="IXF98_000173"
     CDS             complement(73281..73946)
                     /locus_tag="IXF98_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554514.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74093..74698)
                     /gene="dhaL"
                     /locus_tag="IXF98_000174"
     CDS             complement(74093..74698)
                     /gene="dhaL"
                     /locus_tag="IXF98_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1554515.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74726..75709)
                     /gene="dhaK"
                     /locus_tag="IXF98_000175"
     CDS             complement(74726..75709)
                     /gene="dhaK"
                     /locus_tag="IXF98_000175"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1554516.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(75711..76106)
                     /gene="dhaM"
                     /locus_tag="IXF98_000176"
     CDS             complement(75711..76106)
                     /gene="dhaM"
                     /locus_tag="IXF98_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1554517.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76260..76580
                     /locus_tag="IXF98_000177"
     CDS             76260..76580
                     /locus_tag="IXF98_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1554518.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76692..77561)
                     /locus_tag="IXF98_000178"
     CDS             complement(76692..77561)
                     /locus_tag="IXF98_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1554519.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(77657..78961)
                     /locus_tag="IXF98_000179"
     CDS             complement(77657..78961)
                     /locus_tag="IXF98_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1554520.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(78958..79467)
                     /locus_tag="IXF98_000180"
     CDS             complement(78958..79467)
                     /locus_tag="IXF98_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554521.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79642..81777)
                     /locus_tag="IXF98_000181"
     CDS             complement(79642..81777)
                     /locus_tag="IXF98_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1554522.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81860..83515)
                     /locus_tag="IXF98_000182"
     CDS             complement(81860..83515)
                     /locus_tag="IXF98_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1554523.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKKLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83517..83831)
                     /locus_tag="IXF98_000183"
     CDS             complement(83517..83831)
                     /locus_tag="IXF98_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1554524.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84001..84261
                     /locus_tag="IXF98_000184"
     CDS             84001..84261
                     /locus_tag="IXF98_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1554525.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84275..84589
                     /locus_tag="IXF98_000185"
     CDS             84275..84589
                     /locus_tag="IXF98_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1554526.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        25040 a        17189 c        15079 g        27398 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgctttc tttttgggtt attgacgtcg
     7381 cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc ttcggttatt
     7441 gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt atctgcttta
     7501 ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga ccgccgtatg
     7561 atcggtacga tcgggacatt gatattattt acatactata tcattatttt ttatacccgt
     7621 ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat tctatatctg
     7681 tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa attagctgat
     7741 cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct agaaaaaaac
     7801 tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc tcttcacagc
     7861 tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac gatattacct
     7921 acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa tatgaaagtt
     7981 cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa agggttgatc
     8041 gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt aaaccttgta
     8101 cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc aaagaagaaa
     8161 aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt gatcaataca
     8221 acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac aaaaacaaat
     8281 ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac caatccttat
     8341 cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta tatacaaata
     8401 aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc aaaacaatta
     8461 gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga cagttatgtc
     8521 catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag aacttcttct
     8581 tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg tgtagcgatc
     8641 ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac ttcccatacg
     8701 gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat cgtcaaagaa
     8761 gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt cgaagatttt
     8821 caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga gatcaaattt
     8881 ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca taaactggaa
     8941 gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat cgagaaaatg
     9001 catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa tgtgcggtcg
     9061 attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc agcctctatc
     9121 cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg actaaatgac
     9181 cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat ttcctgtgca
     9241 atttcacgtc catccattgt atctggataa aatgttggcc aattctccat ttctttcagc
     9301 aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc agccggtgca
     9361 atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc ttctgcttcg
     9421 aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt ccctacataa
     9481 cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc tttctcactg
     9541 atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt atattcttgt
     9601 gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa agtcccatct
     9661 tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga gcgatcttgt
     9721 acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg ggtcaaagta
     9781 tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt tgcttccatg
     9841 atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc caaaggattc
     9901 aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact atctttggcg
     9961 ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt cagttcagca
    10021 attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac ttgattcaat
    10081 ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga tctttgtttt
    10141 gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc ggtataggct
    10201 gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag ctgatcacgt
    10261 atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg gtctagctca
    10321 tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc acttcccgga
    10381 agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg taatgttttt
    10441 tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat atctgtatct
    10501 tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt ccctgctgga
    10561 atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc atacatggga
    10621 taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc acttgccccg
    10681 caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa tcgtttcatt
    10741 tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt ttcgcaaacc
    10801 gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag aactgtcaag
    10861 cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata tttttgcaat
    10921 caacctctat cattttcctt ttatacccgc ttttgtctat aatgaagtta tagataaagg
    10981 aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct ttttaggagg
    11041 aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca tttatttttt
    11101 ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta tcttgccccc
    11161 attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac gtttgagcaa
    11221 gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat ttttattggg
    11281 gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat tgatcagtag
    11341 tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg agaaaacacc
    11401 ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt ctgatatagc
    11461 tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca ttttttcaac
    11521 tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt tccttttgag
    11581 agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat ttcgtacaga
    11641 ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa aggggcaaat
    11701 cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga tcggattaga
    11761 atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat atattgggcc
    11821 gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt ttatggctgt
    11881 caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg tgattcctcg
    11941 agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt tacttgtcat
    12001 gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata cgttagtcaa
    12061 attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat tttatggcga
    12121 taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat aaaacacaaa
    12181 acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt tttaattttt
    12241 cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa cagtatcgga
    12301 ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga tccttgtatc
    12361 tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc gaagaaagcc
    12421 ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa cgattggata
    12481 tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt cagaagatag
    12541 ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag gtttccctgt
    12601 agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt tatcgcgaga
    12661 ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa ggagtaactt
    12721 ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt ctgggtcgat
    12781 catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat ggatcatggt
    12841 tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc gattgagcgc
    12901 acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct cactcatctt
    12961 ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag gagtagacca
    13021 tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca ttgataattc
    13081 ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc tcatttcacc
    13141 gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt ttagatacgt
    13201 atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat tatttttcaa
    13261 ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta cccctttttc
    13321 catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc gccagttcaa
    13381 gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc caaatggcga
    13441 ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttctctta aaatgaagaa
    13501 gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt ctgtcgaaac
    13561 gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt cggctattag
    13621 ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat cagagatatc
    13681 ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca tgtcgatgcc
    13741 tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa ttccatcacc
    13801 attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt taaatgtcat
    13861 ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat acaagttatc
    13921 atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac attctaagag
    13981 gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct cttgtttaaa
    14041 atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat ctacggcaat
    14101 cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct ttttgctcga
    14161 tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt ccttcggtat
    14221 tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc taatactcag
    14281 gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg catcaatcat
    14341 atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa atacgatagt
    14401 taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc
    14461 catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat
    14521 gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc
    14581 aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt
    14641 agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac
    14701 aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga
    14761 cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt
    14821 tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga
    14881 agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt
    14941 tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga
    15001 taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag gcaaggagcg
    15061 tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg
    15121 tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt
    15181 atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg
    15241 acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcgttcc
    15301 aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg caaccatcag
    15361 cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag
    15421 atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc
    15481 aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc
    15541 tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agaatgcggc
    15601 agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg
    15661 ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa
    15721 aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac
    15781 tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg
    15841 tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag gaaaaacatt
    15901 ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga
    15961 acgttcattg tataggtatg atggtttagt taaattttca attttttctg catttcgatt
    16021 caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc
    16081 agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg
    16141 aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt
    16201 tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc
    16261 ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt
    16321 ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa
    16381 attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga
    16441 ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc
    16501 caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt
    16561 caattcatct gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt
    16621 ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt
    16681 gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt
    16741 ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc
    16801 aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt
    16861 accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa
    16921 taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg
    16981 aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac
    17041 aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt
    17101 aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac
    17161 ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc
    17221 aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc
    17281 acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa
    17341 tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac
    17401 ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg
    17461 gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc
    17521 aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat
    17581 attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt
    17641 ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg
    17701 tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt
    17761 ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac cactgaattt
    17821 ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt tatgatttac
    17881 ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta aaggaaaata
    17941 tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa gagatcgtaa
    18001 ataacggtac actctctcca cacactgcag catctattct gacaaacaat gatttgttag
    18061 taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac atctccactt
    18121 tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta cctccagaat
    18181 tcactgattt acttttgaag gattctttga aagatacaga taatatgttg actatcacaa
    18241 aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc cggcgtggaa
    18301 tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct actgcattag
    18361 atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt gatcgtgaca
    18421 aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga gtacgctcaa
    18481 cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacat gtgattttca
    18541 tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga gtatgaacat
    18601 ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt caatcacaac
    18661 tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg ttcagaaagt
    18721 tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac aaatgattac
    18781 caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat tcttcagtac
    18841 actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga tattatgtaa
    18901 tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac gtcgaggtgt
    18961 ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg aaaatttgaa
    19021 aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat ttagtgatgc
    19081 cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc ttagtctagg
    19141 acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc acgctcggat
    19201 tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac tgatcctctt
    19261 acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc ttgattctgc
    19321 tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc gcggattaga
    19381 ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata atatagatat
    19441 gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg aattgggaca
    19501 aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat gggaagaaaa
    19561 aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat agctttcaag
    19621 gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta aaaaataatt
    19681 ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt tacagtaatt
    19741 ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc aggcgaaacg
    19801 atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca agcagtggca
    19861 gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga cagcgaagga
    19921 caacagttat tagatctact gacaaaagaa aacatcgata catctgggat agctgaaatc
    19981 caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga aaatagtatc
    20041 ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa aaacaagcaa
    20101 ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga tgcgacgatc
    20161 ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcctgaatcc tgcaccagct
    20221 cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc aaatgaaaca
    20281 gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt cgcagcagcc
    20341 gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag tgccggcgct
    20401 ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga tgccgttgac
    20461 acaacagccg caggtgatac atttattggc gcattaagca gcacacttca gccagacctt
    20521 agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc tgtgcagtct
    20581 tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc agtctaaaat
    20641 taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc agatatttca
    20701 cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc aggcatgcct
    20761 gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc aagtttttta
    20821 tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt ggcggaagaa
    20881 ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc agatacaccg
    20941 attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca tgcatttatt
    21001 cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt ggtcttctaa
    21061 tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat atgctgattg
    21121 aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact cggcttcgta
    21181 tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa atcccttcca
    21241 ttaagtattt cattttactc gatagatatg cagaaataat catggatcag ggattggctc
    21301 ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa aagctaaatc
    21361 cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt ccgctgtttg
    21421 ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc tggcttagtg
    21481 aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt tattttctgc
    21541 caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat attgcggctg
    21601 tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac agtggcaaag
    21661 cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct actgtcatgc
    21721 cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa aaatatattc
    21781 gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg gttcctttga
    21841 cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct gccgcaatta
    21901 cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt ggttggaata
    21961 ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt aatatctctg
    22021 cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga atggctggtg
    22081 cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc tccggttctg
    22141 cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga attgcgatcc
    22201 cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga gctgtaatgg
    22261 gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca ttgcctgcat
    22321 tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta gtcgtagcaa
    22381 tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa tagttaatga
    22441 aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt taggatcatt
    22501 atttttataa atttttgtga tactttccaa actttggtat aatttacata tgcataacca
    22561 tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa atgaacgtca
    22621 aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag aatacacaga
    22681 cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa atgtctaatg
    22741 aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta tgaaaaataa
    22801 aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt tgtcgaccaa
    22861 taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag atacgactac
    22921 atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa ctggaacaac
    22981 taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc catcagaaaa
    23041 tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg gtaagatcca
    23101 actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta cggaaagtgg
    23161 aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc cagctgaagg
    23221 tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg atgcaaagac
    23281 ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg gaccggatac
    23341 ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag atccggaaac
    23401 gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata attcaaacaa
    23461 taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat ctggatcgaa
    23521 taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat cgaatggcgg
    23581 aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag agaatccatc
    23641 ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc ctacaaatac
    23701 aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa aaccatatgt
    23761 atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt acgtatatat
    23821 gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg caggtacgca
    23881 gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt cttccggtgt
    23941 tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc ctcaaccagg
    24001 agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg tccgagtaaa
    24061 cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta ttttagatgg
    24121 attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct tgaaaaagat
    24181 tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag tcatgatggc
    24241 tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac caaatcataa
    24301 cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata cacttgaaca
    24361 agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc cttcttacaa
    24421 ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca atcctgattt
    24481 ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca aatatttgga
    24541 aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca tcgaagcgta
    24601 cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag aagcaaacgc
    24661 tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa cagatggcgt
    24721 gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt cttccatctt
    24781 ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga taccagaaac
    24841 aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa gtttagacaa
    24901 atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg gatgtgacac
    24961 tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat tccgttacaa
    25021 actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt cgctccgctc
    25081 ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta ttcccgcgca
    25141 tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa gcatctgttt
    25201 ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt agtaaaccag
    25261 attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat tggcgaaaat
    25321 cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct gtgccactta
    25381 ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat aatcggctat
    25441 tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa ttgaaataga
    25501 catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc gtcactcgcc
    25561 atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca ggaaatccgt
    25621 tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca ccattgatga
    25681 ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa ccaatgacca
    25741 gtgtccgggt ctgtttttac ttcgaacgtt tcatcatcta atttgaatgt gccattcgta
    25801 atccgtccag ccatacgtcc gattgaagca ccgatataag ggtctttttc tagatattct
    25861 tttgcttcat caaaacctaa gatcaactct cgtttttcat tatcgattat tgtttgaaaa
    25921 tgaacaaccc gtgccccaag atcactgaca gacaaagatg tcccattttg attcgtgatc
    25981 gtaatcaagt gatagttttc gccaaaattt tgttcattga tttccatgtt ccttgcaccc
    26041 tcttttcttt catttgcact gattccagca tacatggaaa ccgttttctt aacagcttat
    26101 tccgtgagta acgagaaaaa atttactata ttctaataaa agaccgtgaa aaaaaggttg
    26161 tgacaaaaat attgtcacaa cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct
    26221 gcggtaataa gcccaaccaa acgaaaaata tgaggagcta tttttgcttg gctgttctta
    26281 ttacttgatg caggacagct ttttcacaat ctctttttca atacgattcc agtatataac
    26341 catgtcgtca gtacatagta gatgaaatac aatacgaaga atatcgtaaa cggaacccaa
    26401 atatctttgt atggattttg catgaataga ccaaacattt gtaatccgaa taagacatgg
    26461 actacaccta agattcctgg tgctaagaat aacacgccta cttctctacg gatcgattgt
    26521 ttaagcaacc cgcgtcgtgt tcctatcttt tcaagcattt cataacgagc aatgtcactc
    26581 ttgcttcctg acaagatctt gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa
    26641 cctaggaaga atcccataaa ttcaaaacca gagaatagac cattatacac ttcatacatt
    26701 tcatatttct ggttgctgta ctctactttt tgcaaagatg gattgttcat tttattttga
    26761 gcaacaagtg tttcgattgg tttcaggttt gcttggaagt cttttacctg aactagctgt
    26821 aaagtggatt gtggcaagtt cagttgatca aattctgatt gactgactac cttgatttct
    26881 ttttcttgct gttccggcaa ttcatagctt cgaagttcct cttgtacagt cagttcattc
    26941 atttttttag caggaacttt ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt
    27001 atcatgatca atggttgagc cgcaaattca cttgcattat agtaaatcgt tcccgcatct
    27061 tctttttgag tataagaagc agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct
    27121 tctgcccgag gattattcat cactagatca tatccagaat tgttttctgt caagtctgca
    27181 atttcatttt taaaacctaa tcctaccgtc aaagcgccca gtgccaaggc aaacagcata
    27241 gcgaccattg agagcatttg tgtatattca cgaatacgaa agtttaactg tgacaatgtg
    27301 aaattgttca attttttcaa tgaaatctgt tctgatttct ttagtaatga taaaaagaaa
    27361 atgatcacag aatggaaaat gaaataagtt cctaatacga tcgtaactaa ggctacccct
    27421 ataccaaaga cttgataggt ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa
    27481 gcaattccca gtactacttc aaggaaaaac aagaatttat gctgcttgat tcttgttggt
    27541 gtctgatccg cacgaatcaa tgctaaaatc ggttttttga caatcgaaaa agcattgaca
    27601 acagcagcta ataaaaacaa aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata
    27661 ctaaaaggtg taaaatgcga aatattgaga tctaactgat ccactaataa tcggtggacg
    27721 atacttgtca atcctacacc gacagctgaa cccactaaag tagctgcgat ccctactaag
    27781 aatgtttcag aaaaaatcat ctgtgcgatt tttctgcctt ttgctcccag catcataaac
    27841 attgcatagt ctttttgccg catcgtcatc aaaaaggaat ttgcatataa aatataaacg
    27901 aatgtgatga tccctagtaa aacagaaccg aatcggaaaa tgataactgt agccgcaacg
    27961 atcgtattac ttttcaaaaa agcttcgttg cttgccattg attcaaacat gtaaaaaata
    28021 gcagatgcca taactaatcc tgagaataac accaagtagt ctcttaaacg gctcttgatc
    28081 ccagtaagtg ataatttcca aagcatagtc aatctccccc tactgttcca agtttccaag
    28141 aatagatagt atttctcgat agaaggaatc acggctttga tctccacgtt tcacttcttg
    28201 atggataccg ccatccttga tgaataaaat acgttggcaa taacttgcag aaaacggatc
    28261 atgcgtcaca agaagaatcg atacatgatc ttttgtattc aattcgtcca tcgtatctaa
    28321 aagatcccgg gcacttttgg aatccagcgc acctgtaggc tcatccccta atagaatcgt
    28381 tggttgtgtg atcaatgcac gtgcggcggc gacccgttgt ttttgtccac cggaaatctc
    28441 agaaggatag ctttccaaaa tatgagagat ggataaacgt tctgcaattt tttggacacg
    28501 ttgcttgatt tcttttggtc tgacgccttg taaagaaaga ggaacagcga tattttcttg
    28561 tgctgtcaag ctttctaata aattgaaatc ttggaaaata aagccaatct cattcgcacg
    28621 aaaatctgct agttgatttc cttttaatgt ggtcacatct ttttgattga ttcggatatg
    28681 cccatctgtt ggtttatcta atgtggataa aatattcaaa agtgtcgact taccagaacc
    28741 agaagcgccc atgatcccaa taaattcgcc tttttctaca tcgaagctga tgccgcttaa
    28801 tgcttgcgtc tttttctcat tgttttttcc gtatactttt gttacattct gtacttcgac
    28861 tattttgttc atttctttcc ctacccttta attagttttt ccagtgctgt tttcggcacg
    28921 tctttttttt cgacttcttc atacgaagtg acacctttat ttttgttcca ggtgattttt
    28981 aaatatgctc ctttacgaag cggtcgagtc aattgtccct tgaagtccag ttctttttcc
    29041 ttaccctctt tcgtatagcc aggcaggata tatctatagt tgactgtttc ttgtccagag
    29101 tctgattttg aaactgtttt tgtaccatct gtcgtaattt gtacatagta ttcttctcca
    29161 cccataacta cgtaatctgc gattttcacc cctgcaagta ccacaacgat gaaaacaagt
    29221 agaccgatca gaagtttttt cactttttcc ccttctctct tactttatat actgattata
    29281 gaaaaaagcg aacaattctg ccttcgaatc aactatcatt cttctttcaa ttttgtcatt
    29341 ttctttaaaa caactaaaaa aagagttcaa gacaataatc cgtcctaaac tccttcttcc
    29401 tatcttacaa aaagtatgtc acttacggtc tttcatgtcg cctgtattga taacaggttg
    29461 cgttcctttg tctgtaatga ttgagacaag taaattattg atcaattgga ctttccgatc
    29521 atcggtgaaa ttgatttctt gtccatcttc gatttgctct aaggccattt gagtgattgt
    29581 gacagctcct tcaacaatgg tttgtcgagc agacaaaatg gcttttgctt gttgacgttg
    29641 gagcatggca ctagcgattt ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac
    29701 ttcgacacct gctactgcta aacgctcttg cagctctttt tgcaactcgt cagacactgc
    29761 cgtcgtgttt cctcgaagcg tcaaatcatt ctcattgaat gtatcatacg ggtattggct
    29821 agcgatatgc cgaatggctg tctcactttg gatttctaca aaatcttgat aatacgctac
    29881 atcgaataat gcctttgctg tatcaacgac tttaaagact actactgctg agatttcgat
    29941 ggggtttcca tccaaatcat tgacttttaa aacagaacta ttgaaattcc gaactttcaa
    30001 cgaaacagtc attttttgtg ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt
    30061 acctaaatat tgtccaaaaa acaaaattac ttttgcttga ttaggactga ctacagttgc
    30121 cgaacttaac aacaataacg tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac
    30181 tttcgcttgc cacataccta aatagaagaa aaacaatcca actaaagcta atacgaccaa
    30241 tccgacaatc cctgcataac cattgatata aaatgctctt ttttctctca tgctacttcc
    30301 tgcgctcctt tatccttcaa taaatatcta atgttacttt tttaattttt ttttcgaata
    30361 cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca accgcttgcc
    30421 tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca gtgcttttct
    30481 tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag aggcaaatct
    30541 tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa tattcctgta
    30601 tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa atgtttgaaa
    30661 acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta attaaccatc
    30721 actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata agtatgtaat
    30781 ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat atactctttc
    30841 accctgtttt ctttttagat attttctttc ttattttact atagttatgt gcgttaagcg
    30901 atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata tccagttgag
    30961 tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt
    31021 tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt attggctggt
    31081 agtctttttc aatataagat ttaccagacg tttggatagt atagccactg ttttccatct
    31141 catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg atcaaactgt
    31201 cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca ttggcctcat
    31261 ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta aattcattga
    31321 acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact tcgttgatat
    31381 caattgcact attattccct tcgattgtca attcagataa ttcatgatct gcagggacaa
    31441 caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt gattttttga
    31501 ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct gtcttatcat
    31561 aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga ttttctgcat
    31621 ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata tcgatcaacc
    31681 gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt gcacctgcta
    31741 ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc aatctcaatt
    31801 tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata aaacgtcgtc
    31861 ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct ttcagcaaca
    31921 gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata aagaataatc
    31981 cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat aggccaacac
    32041 ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa gcaaccaata
    32101 gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga gaagcaaata
    32161 aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc actaactcgt
    32221 tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca aactgctttg
    32281 gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa aaatattcct
    32341 cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa
    32401 cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa agaatctcga
    32461 aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg tgtcaacgag
    32521 taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata tccttctttt
    32581 ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact ttcttgtacg
    32641 atttttgtta aggcataccc atacatatct tccttgctta atgctgctag cacagtacca
    32701 tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct caagtgtaat
    32761 attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag tttgtcaaat
    32821 acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg cttctctttg
    32881 atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt ggcatgataa
    32941 cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc attctaaaac
    33001 cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg tctggtgtag
    33061 tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg aattggggaa
    33121 ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct gtgaaaaaat
    33181 caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa tctaaacttc
    33241 catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat gtgctgataa
    33301 attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata ggtacttgca
    33361 tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcaaacca atcgctgttc
    33421 ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg gaatagattc
    33481 ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta
    33541 atttatttcc tactaatccg ccaattagtc cgccaaacaa aacggaacct acattgatca
    33601 aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa aaatcagaaa
    33661 aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag aagattacac
    33721 caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa tatgatggat
    33781 atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac caaaactgct
    33841 gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt agaaacagaa
    33901 ttagacgtca aattattcga acgcgccaat agaaaaatcg aattgaccga agctggaagc
    33961 ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca agaagtgctt
    34021 gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc gacaatgatc
    34081 aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga atttacagtc
    34141 cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga acatagtgac
    34201 acgatccact ttgctagaag tttccaggcc ccaccaagtt cgattgagtg gttgcctact
    34261 gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag taaaaagaaa
    34321 ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac tacgaacttg
    34381 tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa
    34441 ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat tgctattgtt
    34501 atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc tatttctcca
    34561 acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt ggcttcagat
    34621 acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct gtccatctat
    34681 ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc atgtccttgt
    34741 ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt tttatgccaa
    34801 aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc taaaaacctt
    34861 caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg tatcattgct
    34921 gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag ttctagaatt
    34981 tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt atctccttga
    35041 taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc tgaatcggtc
    35101 acttccagta caatctctgt ggctgtctca aatagtttga tatgaatctg tccattgatt
    35161 tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa aaactcttca
    35221 ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc tttctcccat
    35281 ttaggctgaa aaaaaacgat tacttctctg atttgttcat ctaatcgata ctctttcttt
    35341 tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc tgttaattgt
    35401 gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc ttcgtcagag
    35461 agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg tgttttgaat
    35521 tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt gagttcttgg
    35581 accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg ctgttcatcc
    35641 attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag cgcactgatt
    35701 ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac catactgaac
    35761 cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc aggcgagata
    35821 gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac taagatcgaa
    35881 atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt tttcatcttt
    35941 aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct tccgtctttt
    36001 caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct gtattcgtat
    36061 ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg ggataagaca
    36121 gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc tgatcttgta
    36181 acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta ctgctgtaat
    36241 tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg acatcgatgg
    36301 gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg
    36361 cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat gagtctagca
    36421 agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct atacgtgtcg
    36481 cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa aagcccgctc
    36541 gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact actaaaatac
    36601 gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg tagttcatct
    36661 tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat tagtataccg
    36721 aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa aaaaatatta
    36781 taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa
    36841 agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat
    36901 tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt ctactaaaga
    36961 ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca tttttcaaag
    37021 ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga tgacattaag
    37081 tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc gagtcggctt
    37141 gacaaatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc agcgagtggc
    37201 catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac caaccggagc
    37261 gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat cgaaagaaaa
    37321 attagtgatc atggtcacac ataatcctga actggcccat caatacgcag atcgaatcat
    37381 tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac gaccaaaaga
    37441 tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa agctttcctt
    37501 caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt ccagtattgg
    37561 gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac aaatcgataa
    37621 cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa cgactaacca
    37681 gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca gcaaaacgat
    37741 tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg atcaaacata
    37801 cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat ttacccgtac
    37861 tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa
    37921 tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa tgacaggtgt
    37981 gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt taaaagacaa
    38041 ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga
    38101 cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga
    38161 cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa acaacaatac
    38221 ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg atgcgatgta
    38281 tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat cttcttctac
    38341 aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa
    38401 cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac
    38461 tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag gtggagacag
    38521 cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa agatcttgga
    38581 ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt atactgattt
    38641 agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt atgtccttat
    38701 tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca ttacttatac
    38761 atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa
    38821 agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt ctgggatatt
    38881 aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat acaacatgac
    38941 ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag tgatcgtctc
    39001 tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga aaaaagacgc
    39061 cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt gagtcataag
    39121 agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc
    39181 tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg taccacaact
    39241 cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct tcaatcgtta
    39301 cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt tttcctcttc
    39361 ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat ttcacgtatt
    39421 ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc acttttactg
    39481 ctttaggaaa tgactcctcc atttttttca ctttccttcc tccaacacta agtatgcgct
    39541 ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc atccattcca
    39601 ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct tttactatag
    39661 ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg atttaagaaa
    39721 agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata ttgaaatcga
    39781 tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta cagtaaaacg
    39841 tccgactcaa gaaggaccaa ctatgacttt caacaatatc aaaggtttcc ccggcacacg
    39901 tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc accatgatta
    39961 tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt
    40021 agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg cagacttcga
    40081 tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc catacatcac
    40141 aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa cgatccatcg
    40201 tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg gacgccacat
    40261 tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca ctatcaatat
    40321 tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca ctccacttgg
    40381 ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg
    40441 tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag cagaaatcat
    40501 gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa tgccagaatt
    40561 tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca aagcagttac
    40621 tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag aacacgtatc
    40681 tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag ctattccagg
    40741 aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga ccatcatgca
    40801 aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta ttcttgcttt
    40861 atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg atattttcga
    40921 catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag atatcatcgt
    40981 gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat ataatcctgg
    41041 ctctattcgt ttccgtggga taagtgcaaa aactatcttt gacggtacag ttcctttcga
    41101 tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg aaaaatattt
    41161 gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg aaaatggaac
    41221 aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt
    41281 atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca cacggaatca
    41341 tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta actgaattcg
    41401 aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc gccagcggat
    41461 catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc gcaggtatct
    41521 ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg aaggagcaac
    41581 gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg gaaaacttga
    41641 ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt tataatcagc
    41701 ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat caatttgaaa
    41761 ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga aacgatagtt
    41821 gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg taaagactta
    41881 ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac attttctcgt
    41941 gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca taaagacgat
    42001 gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc
    42061 acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg
    42121 tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt ccatatcaaa
    42181 gaaccagttt attataaaga tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag
    42241 cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat tttgccgttt
    42301 ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt gtgataaagc
    42361 cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat ttttgcataa
    42421 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg ttgtgacacg
    42481 tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata tcaccgattt
    42541 cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag actgaaccat
    42601 ctggattttt cacgcccata ttcacgatac tgccttcata tggcacattg tcaaaagttg
    42661 cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga aaaataatat
    42721 atgccccgcc cttgccgact tcggaagcat agatttttga ttcaaaagaa taaatttccc
    42781 tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa gaaggaactg
    42841 tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct gccaactcct
    42901 cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat tgctccttgt
    42961 gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca aaatattatt
    43021 cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca gcagctaagg
    43081 ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc attgctttta
    43141 ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc tgtttcttca
    43201 ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc tctgggattt
    43261 tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg aaccagaatt
    43321 tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca gcatcgccgt
    43381 ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt
    43441 tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct ttatacaaaa
    43501 gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt gtttccgtta
    43561 ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt gaagcgacga
    43621 agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg aacacgttcg
    43681 ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat tatcccccgc
    43741 tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc caagaatccc
    43801 aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat tgattgcctg
    43861 atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat ttgcttttgt
    43921 cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa
    43981 tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat tcataccaga
    44041 agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat aaaggatcac
    44101 gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa
    44161 aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt
    44221 cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc cagaatggaa
    44281 tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg cccctaccat
    44341 cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac
    44401 acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca aggcgatgaa
    44461 aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt catctcctac
    44521 actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag cggataaaat
    44581 atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg ctaaaaccaa
    44641 gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt agttatcaat
    44701 gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga tccctgacat
    44761 ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat tttgcaataa
    44821 tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta atgtactaaa
    44881 attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg cagaaagata
    44941 taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca tgatcccgtt
    45001 gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat cgaaagcttt
    45061 catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc
    45121 aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa tatctacggg
    45181 ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac tagaatactt
    45241 tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg gatataatta
    45301 agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt
    45361 tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta ggtgtgtctc
    45421 cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga
    45481 attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc aaaaaatatt
    45541 tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa
    45601 tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg atcgacgaac
    45661 gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg tacatcattc
    45721 atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa aaagctgact
    45781 acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca agctttttaa
    45841 taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga atatccgaac
    45901 ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg atattttttt
    45961 atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat tctgtttccc
    46021 gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt tctttttcag
    46081 taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca tgaaggtcac
    46141 gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg aaatacaatt
    46201 tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga atccactctg
    46261 ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat ttttcacctg
    46321 tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt tcaggataaa
    46381 tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat tgattcaatt
    46441 catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga agtgtatttt
    46501 ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc ataaatgagt
    46561 aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt aaacatgaaa
    46621 gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta tgacaatggt
    46681 tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat ccatggcgga
    46741 atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt gacggacggc
    46801 gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga ctttatgcca
    46861 aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga acatccagaa
    46921 atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat
    46981 gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat gggtgatgca
    47041 ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc agatgatatc
    47101 cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg tgaaagagag
    47161 gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa tagttcctca
    47221 tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaga
    47281 ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa atagcttttc
    47341 atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac cgtttatcaa
    47401 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa aaatagttcc
    47461 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    47521 aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat gaaataatgc
    47581 tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca aaaatcagcg
    47641 ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt ttgctgtgtt
    47701 tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt ttgatagact
    47761 gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt accgattctt
    47821 tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat caccccagca
    47881 aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg taaagctttc
    47941 ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg agaatccaag
    48001 ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa ttttgttgtt
    48061 gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa accatttttt
    48121 tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg ttttactttt
    48181 ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg ataagttcca
    48241 ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga aaacggcaca
    48301 ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc atttaccggt
    48361 ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact taatctatac
    48421 tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat cgttcctgca
    48481 agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc tttttttacc
    48541 gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt gatcatatac
    48601 ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag aacagacgtg
    48661 tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc ccccaaaaca
    48721 gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa ccgtcgatta
    48781 ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag
    48841 gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg atcataaagt
    48901 gaaatattta attggatctt aggtatattg atacttccga tcagccattc ctctaattgg
    48961 ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc aggattgaat
    49021 ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac ttcaatttca
    49081 gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact atttaaagaa
    49141 tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat taataaaaac
    49201 aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg tgtcctccct
    49261 tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata gaaaatgaaa
    49321 gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc caaaaaaata
    49381 gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    49441 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
    49501 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
    49561 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
    49621 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
    49681 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
    49741 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
    49801 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
    49861 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
    49921 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
    49981 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
    50041 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
    50101 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
    50161 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
    50221 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
    50281 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
    50341 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
    50401 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
    50461 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
    50521 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
    50581 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
    50641 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
    50701 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
    50761 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
    50821 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
    50881 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
    50941 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
    51001 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
    51061 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
    51121 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
    51181 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
    51241 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
    51301 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
    51361 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
    51421 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
    51481 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
    51541 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
    51601 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
    51661 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
    51721 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
    51781 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
    51841 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
    51901 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
    51961 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
    52021 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
    52081 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
    52141 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
    52201 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
    52261 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
    52321 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
    52381 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
    52441 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
    52501 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
    52561 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
    52621 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    52681 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
    52741 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
    52801 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
    52861 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
    52921 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
    52981 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
    53041 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
    53101 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
    53161 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
    53221 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
    53281 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
    53341 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
    53401 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
    53461 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
    53521 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat acgtaacaac
    53581 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
    53641 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
    53701 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
    53761 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
    53821 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
    53881 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
    53941 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
    54001 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
    54061 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
    54121 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
    54181 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
    54241 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
    54301 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
    54361 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
    54421 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
    54481 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
    54541 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
    54601 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
    54661 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
    54721 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
    54781 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
    54841 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
    54901 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
    54961 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
    55021 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
    55081 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
    55141 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
    55201 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
    55261 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
    55321 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
    55381 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
    55441 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
    55501 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
    55561 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
    55621 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
    55681 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
    55741 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
    55801 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
    55861 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
    55921 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
    55981 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
    56041 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
    56101 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
    56161 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
    56221 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
    56281 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
    56341 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
    56401 ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg gcgatacgga
    56461 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
    56521 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
    56581 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
    56641 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
    56701 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
    56761 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
    56821 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
    56881 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
    56941 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
    57001 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
    57061 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
    57121 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
    57181 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
    57241 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
    57301 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
    57361 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
    57421 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
    57481 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
    57541 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
    57601 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
    57661 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
    57721 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
    57781 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
    57841 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
    57901 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
    57961 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
    58021 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
    58081 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
    58141 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
    58201 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
    58261 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
    58321 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
    58381 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
    58441 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
    58501 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
    58561 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
    58621 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
    58681 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
    58741 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
    58801 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
    58861 acttctaata aaagtatggt attattttct acgactggat ttgaagctga tttctctgtc
    58921 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
    58981 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
    59041 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
    59101 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
    59161 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
    59221 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
    59281 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
    59341 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
    59401 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    59461 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    59521 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    59581 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    59641 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    59701 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    59761 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    59821 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    59881 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    59941 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    60001 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    60061 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    60121 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    60181 ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt
    60241 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
    60301 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
    60361 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
    60421 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
    60481 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
    60541 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
    60601 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
    60661 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
    60721 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
    60781 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
    60841 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
    60901 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
    60961 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
    61021 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
    61081 tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
    61141 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
    61201 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
    61261 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
    61321 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
    61381 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
    61441 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
    61501 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
    61561 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
    61621 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
    61681 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
    61741 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
    61801 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
    61861 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
    61921 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
    61981 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
    62041 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
    62101 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
    62161 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
    62221 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
    62281 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
    62341 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
    62401 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
    62461 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
    62521 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
    62581 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
    62641 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
    62701 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
    62761 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
    62821 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
    62881 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
    62941 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
    63001 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
    63061 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
    63121 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
    63181 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
    63241 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
    63301 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
    63361 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
    63421 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
    63481 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
    63541 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
    63601 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
    63661 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
    63721 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
    63781 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
    63841 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
    63901 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
    63961 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
    64021 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
    64081 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
    64141 gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc ggatatcttt
    64201 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
    64261 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
    64321 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
    64381 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
    64441 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
    64501 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
    64561 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
    64621 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
    64681 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
    64741 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
    64801 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
    64861 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
    64921 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
    64981 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
    65041 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
    65101 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
    65161 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
    65221 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
    65281 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
    65341 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
    65401 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
    65461 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
    65521 atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa
    65581 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
    65641 ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc
    65701 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
    65761 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
    65821 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
    65881 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
    65941 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
    66001 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
    66061 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
    66121 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
    66181 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
    66241 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
    66301 gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat
    66361 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
    66421 ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag
    66481 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
    66541 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
    66601 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
    66661 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
    66721 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
    66781 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
    66841 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
    66901 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
    66961 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt aattaattat
    67021 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
    67081 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
    67141 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
    67201 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
    67261 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
    67321 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
    67381 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
    67441 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
    67501 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
    67561 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
    67621 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
    67681 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
    67741 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
    67801 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
    67861 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
    67921 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
    67981 tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag
    68041 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
    68101 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
    68161 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
    68221 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
    68281 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
    68341 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
    68401 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
    68461 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
    68521 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
    68581 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
    68641 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
    68701 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
    68761 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
    68821 cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac tgacatttta
    68881 tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc ttgatcagga
    68941 cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc ttagaaattc
    69001 cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc aaatccactt
    69061 ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt tcttgcgtat
    69121 acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat gtcatcaaat
    69181 cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca ctcatggcaa
    69241 tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct tgccctaaat
    69301 cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca aaatatttaa
    69361 aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc actccagcgg
    69421 ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc tttttctctt
    69481 gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa cttttataat
    69541 atatatagtt caaatttatt ctgaggttta ttataactat ctattgagaa agtatcaagc
    69601 tagcaattcc ttgtttatga attcattgac agaaatttat aaaaagaata ttatgtacta
    69661 aaataataaa aaaagtttta taaaaaggta taaaaccttt ttattaaact atttaatctt
    69721 gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca
    69781 cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac
    69841 attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc acgacgattc
    69901 ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt gggcaagatg
    69961 tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc gatatctctt
    70021 tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt catggaaaag
    70081 ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct aagaaaaaag
    70141 caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct ttggctgttg
    70201 attcggatct ctatttattt gatgagccta cttctggttt agatcctttg atggaaacga
    70261 ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta ctttcttctc
    70321 atattctcag tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc gaaggaacac
    70381 tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc atcactatag
    70441 aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac tttgtgcaaa
    70501 aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt ttagctgaag
    70561 cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag gatttattca
    70621 tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca cgctggtcca
    70681 cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg ctagtcggtg
    70741 tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc ggacaaggat
    70801 tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc
    70861 cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa atgttgcttt
    70921 tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa
    70981 aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt caagcaaact
    71041 ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata tcgatcagtt
    71101 gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg ctttttgggc
    71161 tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct gcacaactga
    71221 tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga ctttaccttc
    71281 tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaaacct ttaggctggt
    71341 cttatttgac ctatccattt acaactaaca actggattcc tttgctttat ttgtttttgt
    71401 tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat ttgaacaccg
    71461 gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg
    71521 gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact tttggtttga
    71581 tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa agtaacgact
    71641 taatgaaaca gatgtttatc caatctggcg tctcaattaa aaaaacttta caagcacgat
    71701 catggtcgtc ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt
    71761 tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca
    71821 actttattgg accaatatgt tactagctat tctatttgct atattggcaa tcctgattag
    71881 ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct
    71941 attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat
    72001 tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc
    72061 ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa
    72121 aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt
    72181 ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg
    72241 aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc
    72301 tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct
    72361 ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat
    72421 ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt
    72481 ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca
    72541 gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt
    72601 aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt
    72661 tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat
    72721 aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt
    72781 actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat actgccggag
    72841 gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca
    72901 tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac
    72961 aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag
    73021 ctatttctaa taaagaaaca taacttccct tattccaact gataattttc ttttatcata
    73081 gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc
    73141 tttattttcc ttcttttgag accttaagta attgattcct atcaattttt taaatattta
    73201 ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg
    73261 cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt
    73321 gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag
    73381 aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt
    73441 ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt
    73501 tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca
    73561 agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac
    73621 atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct
    73681 tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc
    73741 cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg
    73801 agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc
    73861 ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac
    73921 tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta
    73981 cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat
    74041 aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt
    74101 gattgatcac atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga
    74161 tatgtccgat tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt
    74221 tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct
    74281 ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc
    74341 tttgaatacc cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag
    74401 aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag
    74461 aagccccacc gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg
    74521 tttcaggatg atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat
    74581 ttccatggtc tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat
    74641 tgattgcctc tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat
    74701 tatcaccctt ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag
    74761 aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta
    74821 gtgaggtcat aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca
    74881 gagctaaaac atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt
    74941 tgactagtac acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa
    75001 gttcctttgc cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt
    75061 ggataccaac accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt
    75121 ctggtacagt tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt
    75181 ctcccagttc tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt
    75241 tgtgtacaag gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa
    75301 cggcgatatc atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt
    75361 cctttgccat atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc
    75421 cttttccact atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa
    75481 acacatcacc aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg
    75541 gttcatgtcc gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg
    75601 ctacaacacg ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt
    75661 ccagcatttc ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat
    75721 cctttctttc cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg
    75781 gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt
    75841 tgctgctgct cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg
    75901 atatccgcaa aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt
    75961 ttcatcgggt ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct
    76021 tcggattttt gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga
    76081 gaaataagca aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg
    76141 attggtttcg ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa
    76201 aatatgtgta gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa
    76261 tgattacaac aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg
    76321 tttttggtga ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa
    76381 atatctttgg cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag
    76441 cacttgccga aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa
    76501 aaatggacta tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa
    76561 cagctgtctc cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac
    76621 atgtagtggc actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca
    76681 tcctctcttt tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc
    76741 gcccctgcta aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga
    76801 cttgttgatt tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc
    76861 actgattttt ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag
    76921 tcagcttttt ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg
    76981 attgcttgac tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt
    77041 tgagcaacct gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga
    77101 tctgtttgga cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca
    77161 cggtcatttc ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct
    77221 tctttcaatt tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc
    77281 aagtcttgca aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct
    77341 gaagatgaaa tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc
    77401 gaggcaccgt tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt
    77461 tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc
    77521 cagattaacg gcttcattct ttttttcatc acttttccca tccttactac tttttaacta
    77581 atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta
    77641 aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat
    77701 aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta
    77761 tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat
    77821 catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga
    77881 acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat
    77941 gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac
    78001 tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca
    78061 cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc
    78121 gatatagcat cttcaacgtc acaggaactt ccatgaaaaa gataatctca ggagcaaaaa
    78181 cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat
    78241 cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac
    78301 tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac
    78361 ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat
    78421 ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa
    78481 tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg
    78541 caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca
    78601 cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg
    78661 aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact
    78721 cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg
    78781 gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat ataaacacat
    78841 aacaccagac accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa
    78901 aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca
    78961 tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt
    79021 ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc
    79081 cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa
    79141 agatttattt acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt
    79201 aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt
    79261 ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa
    79321 aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg
    79381 ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc
    79441 aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag
    79501 gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa
    79561 aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc
    79621 tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc
    79681 tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa
    79741 tggatataag attacatgat caaattccag tccttttgct agactcaaag gaatgatttg
    79801 gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc
    79861 ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag
    79921 tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg
    79981 tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt
    80041 gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta
    80101 gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt
    80161 ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg
    80221 tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac
    80281 cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt
    80341 gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg
    80401 ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa
    80461 aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa
    80521 taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt
    80581 tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag
    80641 cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt
    80701 agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa
    80761 caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg
    80821 ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc
    80881 tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa
    80941 tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa
    81001 gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat
    81061 agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc
    81121 ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt
    81181 ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa
    81241 aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc
    81301 gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc
    81361 ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc
    81421 tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc
    81481 aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg
    81541 tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag
    81601 gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg
    81661 gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag
    81721 cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca
    81781 cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat
    81841 gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt
    81901 gatataaaga aaatgcacca tcaagttttt ttacgtggta gccagactgc ttcaagattc
    81961 tttccgctag atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt
    82021 ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt
    82081 tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt
    82141 ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg
    82201 catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt
    82261 ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga
    82321 tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg
    82381 gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt
    82441 ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta
    82501 aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt
    82561 gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg
    82621 gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat
    82681 cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc
    82741 cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa
    82801 tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc
    82861 cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca
    82921 attctttttc tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt
    82981 gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga
    83041 tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt
    83101 tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa
    83161 tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt
    83221 gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt
    83281 catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa
    83341 gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa
    83401 aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa
    83461 gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat
    83521 ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca
    83581 taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc
    83641 acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt
    83701 ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct
    83761 ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat
    83821 agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa
    83881 acaggagctt tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat
    83941 atttgcataa aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt
    84001 atggagattg ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt
    84061 ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc
    84121 gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat
    84181 tgctttgaga aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc
    84241 aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga
    84301 attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga
    84361 aacgacgatt gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa
    84421 tgcagaattc ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc
    84481 taatcatatt tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct
    84541 tgatttagtt gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt
    84601 tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg
    84661 gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca tcaagt
//
LOCUS       DACETN010000003        70988 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DACETN010000003 DACETN010000000
VERSION     DACETN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.003"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            336..1439
                     /locus_tag="IXF98_000186"
     CDS             336..1439
                     /locus_tag="IXF98_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1554527.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IXF98_000187"
     CDS             1723..2403
                     /locus_tag="IXF98_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1554528.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IXF98_000188"
     CDS             2493..3527
                     /locus_tag="IXF98_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1554529.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IXF98_000189"
     CDS             3541..3876
                     /locus_tag="IXF98_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554530.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IXF98_000190"
     CDS             3860..6340
                     /locus_tag="IXF98_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554531.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IXF98_000191"
     CDS             6396..7955
                     /locus_tag="IXF98_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1554532.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IXF98_000192"
     CDS             7952..9316
                     /locus_tag="IXF98_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1554533.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IXF98_000193"
     CDS             9487..10557
                     /locus_tag="IXF98_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554534.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IXF98_000194"
     CDS             10572..11240
                     /locus_tag="IXF98_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554535.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IXF98_000195"
     CDS             11260..11838
                     /locus_tag="IXF98_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1554536.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IXF98_000196"
     CDS             12241..13152
                     /locus_tag="IXF98_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1554537.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IXF98_000197"
     CDS             complement(13408..14325)
                     /locus_tag="IXF98_000197"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1554538.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IXF98_000198"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IXF98_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1554539.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15570
                     /locus_tag="IXF98_000199"
     CDS             15022..15570
                     /locus_tag="IXF98_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1554540.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15685..18045
                     /locus_tag="IXF98_000200"
     CDS             15685..18045
                     /locus_tag="IXF98_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1554541.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18132..18446
                     /gene="trxA"
                     /locus_tag="IXF98_000201"
     CDS             18132..18446
                     /gene="trxA"
                     /locus_tag="IXF98_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1554542.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18628..20406
                     /gene="uvrC"
                     /locus_tag="IXF98_000202"
     CDS             18628..20406
                     /gene="uvrC"
                     /locus_tag="IXF98_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1554543.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20551..21450
                     /locus_tag="IXF98_000203"
     CDS             20551..21450
                     /locus_tag="IXF98_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554544.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21443..22705
                     /locus_tag="IXF98_000204"
     CDS             21443..22705
                     /locus_tag="IXF98_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554545.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22868..24766
                     /locus_tag="IXF98_000205"
     CDS             22868..24766
                     /locus_tag="IXF98_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1554546.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25065..25784)
                     /locus_tag="IXF98_000206"
     CDS             complement(25065..25784)
                     /locus_tag="IXF98_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1554547.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26121..26453
                     /locus_tag="IXF98_000207"
     CDS             26121..26453
                     /locus_tag="IXF98_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554548.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26440..28443
                     /locus_tag="IXF98_000208"
     CDS             26440..28443
                     /locus_tag="IXF98_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1554549.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28462..28932
                     /locus_tag="IXF98_000209"
     CDS             28462..28932
                     /locus_tag="IXF98_000209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1554550.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28953..29537
                     /locus_tag="IXF98_000210"
     CDS             28953..29537
                     /locus_tag="IXF98_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1554551.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29552..30538
                     /locus_tag="IXF98_000211"
     CDS             29552..30538
                     /locus_tag="IXF98_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1554552.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30528..30839
                     /locus_tag="IXF98_000212"
     CDS             30528..30839
                     /locus_tag="IXF98_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1554553.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30861..32642
                     /locus_tag="IXF98_000213"
     CDS             30861..32642
                     /locus_tag="IXF98_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1554554.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32635..34011
                     /locus_tag="IXF98_000214"
     CDS             32635..34011
                     /locus_tag="IXF98_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1554555.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34018..34653
                     /locus_tag="IXF98_000215"
     CDS             34018..34653
                     /locus_tag="IXF98_000215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1554556.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34683..36038
                     /locus_tag="IXF98_000216"
     CDS             34683..36038
                     /locus_tag="IXF98_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1554557.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36230..37624)
                     /locus_tag="IXF98_000217"
     CDS             complement(36230..37624)
                     /locus_tag="IXF98_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1554558.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37716..38687
                     /locus_tag="IXF98_000218"
     CDS             37716..38687
                     /locus_tag="IXF98_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1554559.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38684..39466
                     /locus_tag="IXF98_000219"
     CDS             38684..39466
                     /locus_tag="IXF98_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1554560.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39635..40006)
                     /locus_tag="IXF98_000220"
     CDS             complement(39635..40006)
                     /locus_tag="IXF98_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1554561.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40140..40583
                     /locus_tag="IXF98_000221"
     CDS             40140..40583
                     /locus_tag="IXF98_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1554562.1"
                     /translation="MDKPISGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IXF98_000222"
     CDS             complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IXF98_000222"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1554563.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41454..42821)
                     /locus_tag="IXF98_000223"
     CDS             complement(41454..42821)
                     /locus_tag="IXF98_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1554564.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42805..43635)
                     /locus_tag="IXF98_000224"
     CDS             complement(42805..43635)
                     /locus_tag="IXF98_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1554565.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43798..44235
                     /locus_tag="IXF98_000225"
     CDS             43798..44235
                     /locus_tag="IXF98_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1554566.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44311..44931
                     /locus_tag="IXF98_000226"
     CDS             44311..44931
                     /locus_tag="IXF98_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1554567.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45120..45626
                     /locus_tag="IXF98_000227"
     CDS             45120..45626
                     /locus_tag="IXF98_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1554568.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45643..46542
                     /locus_tag="IXF98_000228"
     CDS             45643..46542
                     /locus_tag="IXF98_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1554569.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46629..47513
                     /locus_tag="IXF98_000229"
     CDS             46629..47513
                     /locus_tag="IXF98_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1554570.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47705..47776)
                     /locus_tag="IXF98_000230"
     tRNA            complement(47705..47776)
                     /locus_tag="IXF98_000230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47741..47743),aa:Arg,seq:ccg)
     gene            47836..49170
                     /gene="rpoN"
                     /locus_tag="IXF98_000231"
     CDS             47836..49170
                     /gene="rpoN"
                     /locus_tag="IXF98_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1554571.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49373..50410
                     /locus_tag="IXF98_000232"
     CDS             49373..50410
                     /locus_tag="IXF98_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1554572.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50451..51452
                     /gene="gap"
                     /locus_tag="IXF98_000233"
     CDS             50451..51452
                     /gene="gap"
                     /locus_tag="IXF98_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1554573.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51562..52752
                     /locus_tag="IXF98_000234"
     CDS             51562..52752
                     /locus_tag="IXF98_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1554574.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52856..53611
                     /locus_tag="IXF98_000235"
     CDS             52856..53611
                     /locus_tag="IXF98_000235"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1554575.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53727..55025
                     /gene="eno"
                     /locus_tag="IXF98_000236"
     CDS             53727..55025
                     /gene="eno"
                     /locus_tag="IXF98_000236"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1554576.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55425..56564
                     /locus_tag="IXF98_000237"
     CDS             55425..56564
                     /locus_tag="IXF98_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1554577.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(56631..57206)
                     /locus_tag="IXF98_000238"
     CDS             complement(56631..57206)
                     /locus_tag="IXF98_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1554578.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(57211..58185)
                     /locus_tag="IXF98_000239"
     CDS             complement(57211..58185)
                     /locus_tag="IXF98_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1554579.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(58209..58253)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            58468..59436
                     /locus_tag="IXF98_000240"
     CDS             58468..59436
                     /locus_tag="IXF98_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1554580.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            59549..59827
                     /locus_tag="IXF98_000241"
     CDS             59549..59827
                     /locus_tag="IXF98_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1554581.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            59945..60181
                     /gene="secG"
                     /locus_tag="IXF98_000242"
     CDS             59945..60181
                     /gene="secG"
                     /locus_tag="IXF98_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1554582.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            60293..61039
                     /locus_tag="IXF98_000243"
     CDS             60293..61039
                     /locus_tag="IXF98_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1554583.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            61042..63399
                     /gene="rnr"
                     /locus_tag="IXF98_000244"
     CDS             61042..63399
                     /gene="rnr"
                     /locus_tag="IXF98_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1554584.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            63421..63885
                     /gene="smpB"
                     /locus_tag="IXF98_000245"
     CDS             63421..63885
                     /gene="smpB"
                     /locus_tag="IXF98_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1554585.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            64187..64906
                     /gene="atpB"
                     /locus_tag="IXF98_000246"
     CDS             64187..64906
                     /gene="atpB"
                     /locus_tag="IXF98_000246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1554586.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            64959..65174
                     /gene="atpE"
                     /locus_tag="IXF98_000247"
     CDS             64959..65174
                     /gene="atpE"
                     /locus_tag="IXF98_000247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1554587.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            65265..65789
                     /gene="atpF"
                     /locus_tag="IXF98_000248"
     CDS             65265..65789
                     /gene="atpF"
                     /locus_tag="IXF98_000248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1554588.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            65776..66318
                     /locus_tag="IXF98_000249"
     CDS             65776..66318
                     /locus_tag="IXF98_000249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1554589.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            66341..67897
                     /locus_tag="IXF98_000250"
     CDS             66341..67897
                     /locus_tag="IXF98_000250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1554590.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            67911..68813
                     /locus_tag="IXF98_000251"
     CDS             67911..68813
                     /locus_tag="IXF98_000251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1554591.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            68836..70242
                     /gene="atpD"
                     /locus_tag="IXF98_000252"
     CDS             68836..70242
                     /gene="atpD"
                     /locus_tag="IXF98_000252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1554592.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            70256..70678
                     /locus_tag="IXF98_000253"
     CDS             70256..70678
                     /locus_tag="IXF98_000253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1554593.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23555 a        12436 c        15089 g        19908 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccaatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    15601 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    15661 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    15721 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    15781 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    15841 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    15901 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    15961 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    16021 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    16081 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    16141 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    16201 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    16261 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    16321 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    16381 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    16441 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    16501 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    16561 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    16621 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    16681 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    16741 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    16801 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    16861 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    16921 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    16981 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    17041 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    17101 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    17161 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    17221 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    17281 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    17341 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    17401 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    17461 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    17521 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    17581 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    17641 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    17701 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    17761 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    17821 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    17881 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    17941 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    18001 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    18061 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    18121 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    18181 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    18241 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    18301 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    18361 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    18421 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    18481 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    18541 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    18601 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    18661 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    18721 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    18781 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    18841 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    18901 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    18961 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    19021 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    19081 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    19141 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    19201 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    19261 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    19321 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    19381 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    19441 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    19501 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    19561 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    19621 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    19681 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    19741 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    19801 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    19861 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    19921 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    19981 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    20041 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    20101 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    20161 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    20221 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    20281 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    20341 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    20401 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    20461 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    20521 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    20581 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    20641 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    20701 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    20761 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    20821 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    20881 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    20941 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    21001 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    21061 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    21121 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    21181 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    21241 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    21301 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    21361 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    21421 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    21481 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    21541 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    21601 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    21661 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    21721 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    21781 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    21841 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    21901 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    21961 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    22021 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    22081 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    22141 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    22201 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    22261 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    22321 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    22381 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    22441 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    22501 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    22561 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    22621 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    22681 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    22741 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    22801 atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg
    22861 cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt
    22921 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    22981 tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    23041 aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag
    23101 ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa
    23161 acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa
    23221 atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    23281 actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga
    23341 acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca
    23401 aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc
    23461 agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca
    23521 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg
    23581 gattgccgca cgctacaact atttatttga attagagaaa gaatatggat taaatttacg
    23641 tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa
    23701 caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc
    23761 tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga
    23821 cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca
    23881 gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt
    23941 cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag
    24001 aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    24061 gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga
    24121 attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga
    24181 aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg
    24241 gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt
    24301 tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga
    24361 agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat
    24421 catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg
    24481 aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc
    24541 tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt
    24601 gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga
    24661 gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca
    24721 gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc
    24781 aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg
    24841 aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact
    24901 caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc
    24961 ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    25021 actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca
    25081 aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat
    25141 tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg
    25201 ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag
    25261 gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg
    25321 acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt
    25381 ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt
    25441 cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt
    25501 tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa
    25561 agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt
    25621 tgaggcgctg aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa
    25681 actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc
    25741 gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa
    25801 gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac
    25861 ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga
    25921 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    25981 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    26041 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    26101 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    26161 cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag
    26221 aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt
    26281 tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga
    26341 aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc
    26401 aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    26461 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    26521 atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa
    26581 aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat
    26641 cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    26701 aagaaactcc atttgaaaca tcaagaactt tccttacaag acttggaagc aaattattcc
    26761 gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag
    26821 ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    26881 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    26941 agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg
    27001 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    27061 gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    27121 gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag
    27181 cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa
    27241 tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc
    27301 catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    27361 atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca
    27421 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa
    27481 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg
    27541 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac
    27601 ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg
    27661 atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg
    27721 atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct
    27781 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    27841 ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa
    27901 gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt
    27961 attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg
    28021 ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa
    28081 gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat
    28141 ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca
    28201 ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg
    28261 atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt
    28321 gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt
    28381 gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa
    28441 taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta
    28501 tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt
    28561 gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga
    28621 caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc
    28681 tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta
    28741 ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt
    28801 gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    28861 tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta
    28921 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    28981 agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga
    29041 tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag
    29101 aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag
    29161 aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg
    29221 caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat
    29281 tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt
    29341 atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa
    29401 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    29461 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    29521 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    29581 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    29641 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    29701 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    29761 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt
    29821 ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta
    29881 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    29941 aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    30001 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    30061 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    30121 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    30181 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    30241 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    30301 ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta
    30361 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    30421 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    30481 aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    30541 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    30601 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    30661 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    30721 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    30781 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    30841 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    30901 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    30961 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    31021 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    31081 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    31141 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    31201 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    31261 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    31321 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    31381 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    31441 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    31501 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    31561 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    31621 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    31681 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    31741 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    31801 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    31861 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    31921 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    31981 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    32041 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    32101 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    32161 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    32221 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    32281 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    32341 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    32401 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    32461 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    32521 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    32581 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    32641 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    32701 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    32761 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    32821 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    32881 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    32941 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    33001 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    33061 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    33121 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    33181 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    33241 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    33301 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    33361 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    33421 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    33481 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    33541 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    33601 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    33661 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    33721 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    33781 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    33841 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    33901 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    33961 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    34021 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    34081 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    34141 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    34201 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    34261 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    34321 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    34381 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    34441 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    34501 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    34561 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    34621 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    34681 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    34741 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    34801 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    34861 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    34921 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    34981 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    35041 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta
    35101 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    35161 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    35221 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    35281 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    35341 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    35401 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    35461 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    35521 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    35581 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    35641 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    35701 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    35761 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    35821 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    35881 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    35941 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    36001 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    36061 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    36121 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    36181 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    36241 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    36301 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    36361 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    36421 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    36481 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    36541 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    36601 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    36661 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    36721 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    36781 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    36841 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    36901 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    36961 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    37021 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    37081 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    37141 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    37201 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    37261 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    37321 cttcattggt agagatggtg atggactcaa agacattggg agaattaaat gtgccacttc
    37381 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    37441 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    37501 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    37561 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    37621 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    37681 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    37741 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    37801 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    37861 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    37921 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    37981 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    38041 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    38101 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    38161 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    38221 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    38281 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    38341 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    38401 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    38461 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    38521 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    38581 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    38641 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    38701 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    38761 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    38821 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    38881 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    38941 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    39001 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    39061 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    39121 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    39181 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    39241 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    39301 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    39361 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    39421 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    39481 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    39541 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    39601 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    39661 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    39721 cagtaatctc aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    39781 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    39841 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    39901 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    39961 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    40021 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    40081 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    40141 tggataaacc aatttctggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    40201 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    40261 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    40321 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    40381 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    40441 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    40501 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    40561 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    40621 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    40681 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    40741 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    40801 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    40861 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    40921 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    40981 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    41041 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    41101 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    41161 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    41221 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    41281 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    41341 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    41401 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    41461 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    41521 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    41581 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    41641 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    41701 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    41761 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    41821 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    41881 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    41941 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    42001 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    42061 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    42121 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    42181 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    42241 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    42301 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    42361 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    42421 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    42481 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    42541 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    42601 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    42661 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    42721 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    42781 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    42841 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    42901 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    42961 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    43021 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    43081 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    43141 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    43201 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    43261 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    43321 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    43381 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    43441 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    43501 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    43561 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    43621 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    43681 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    43741 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    43801 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    43861 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    43921 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    43981 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    44041 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    44101 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagg agggaaggtt
    44161 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    44221 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    44281 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    44341 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    44401 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    44461 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    44521 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    44581 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    44641 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    44701 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    44761 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    44821 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    44881 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    44941 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    45001 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    45061 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    45121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    45181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    45241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    45301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    45361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    45421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    45481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    45541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    45601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    45661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    45721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    45781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    45841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    45901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    45961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    46021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    46081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    46141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    46201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    46261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    46321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    46381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    46441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    46501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    46561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    46621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    46681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    46741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    46801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    46861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    46921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    46981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    47041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    47101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    47161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    47221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    47281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    47341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    47401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    47461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    47521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    47581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
    47641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
    47701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
    47761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
    47821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
    47881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
    47941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
    48001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
    48061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
    48121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
    48181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
    48241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
    48301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
    48361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
    48421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
    48481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
    48541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
    48601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
    48661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
    48721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
    48781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
    48841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
    48901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
    48961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
    49021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
    49081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
    49141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
    49201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
    49261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
    49321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
    49381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
    49441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
    49501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
    49561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
    49621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
    49681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
    49741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
    49801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
    49861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
    49921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
    49981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
    50041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
    50101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
    50161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
    50221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
    50281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
    50341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
    50401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
    50461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
    50521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
    50581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
    50641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
    50701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
    50761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
    50821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
    50881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
    50941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
    51001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
    51061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
    51121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
    51181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
    51241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
    51301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
    51361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
    51421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
    51481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
    51541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
    51601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
    51661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
    51721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
    51781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
    51841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
    51901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
    51961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
    52021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
    52081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
    52141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
    52201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
    52261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
    52321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
    52381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
    52441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
    52501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
    52561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
    52621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
    52681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
    52741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
    52801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
    52861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
    52921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
    52981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
    53041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
    53101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
    53161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
    53221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
    53281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
    53341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
    53401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
    53461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
    53521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
    53581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
    53641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
    53701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
    53761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
    53821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
    53881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
    53941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
    54001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
    54061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
    54121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
    54181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
    54241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
    54301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
    54361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
    54421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
    54481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
    54541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
    54601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
    54661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
    54721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
    54781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
    54841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
    54901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
    54961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    55021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    55081 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttttttt
    55141 gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa
    55201 aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga acagtagaat
    55261 aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt
    55321 ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag
    55381 cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa
    55441 aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag
    55501 tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt
    55561 acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt
    55621 taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg
    55681 caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga
    55741 agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat
    55801 ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg
    55861 aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa
    55921 ctaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg
    55981 gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg
    56041 aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg
    56101 tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca
    56161 tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata
    56221 aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt
    56281 tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga
    56341 ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa
    56401 cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg
    56461 gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata
    56521 tgattgaaaa atatcaagga attattttat cctacttagc gtaagatttt tttaataaat
    56581 gaaaaaacag agccagatta gcattatttt gctaatccag ctctgtttat tcagcctatt
    56641 tttctttttg aatatatcaa gcgttgtggc aacatagtga tgactaatga aacaacgacg
    56701 atagacaaca aacgatcaag gtagtctgta cctgcctgta ccaagatcac acttgttgtc
    56761 tgacttaaac ctaacccatg taatagctga acgatcatac tggatcctga ggaggtgatc
    56821 ccgccaaata actgaacggt aatgatcgaa gaaacgatcg ttccaggtac cgacaaaatc
    56881 aaagcgagta ataacagttt caagctgttt ttatctttgc cgagatatct tggcaaaatc
    56941 aatccagcaa gcaagcctgt gagaatttgg attggcgaat aatacagaga aaaaatatct
    57001 gtagtcactc cacttaaaat accgctggac aatcctgtca gcattccagg tacaggccca
    57061 agcaatgcac cagcaaaaat cgttccgata gaatcgagat agattggcaa acgaagccat
    57121 aaagcaatcg ttcctccaat ataattgatc ccaatacaca aagccatcac tgtaagtacg
    57181 cgagttgcca ttttgttttt attcattttg ttagcccatc tttaaccttt ccaaatcttt
    57241 cataatcgtt tcttcttgtg catttaaaag aacggttaaa aactttcgga aaaaaagacg
    57301 agtatctact tttgtcataa ttttactgtt agcaggtttc tgccaaaatt cataacgatc
    57361 aaccaatgtt tgtccacgac ttattccttg agtttccaca gcagtgaagc ttttgaaacc
    57421 ttcacagatg gccccctcga taaaataagc aacagctaac ggatcattga tgacacatcc
    57481 taaaattctt tcttgtttcc agtggaagtc aaaataaaaa cgagtgatcg cttttagata
    57541 ctctccctct tcaggattca tttgacagca gtattcgagg atagtcggtg tcaagacgat
    57601 ttctcttgtc acatctaacc caaccatttc tatcgtttgt ttgagattct cgaatacata
    57661 agaagctgct tcaggatcac accagtaatt gtattcagca actggcgaac agttaccatg
    57721 gcttttgtaa gttccaccca tcgagacaaa acgatccata tgtttcccaa tatttgggtt
    57781 gactttcaaa gcaaaagcga tattggtcaa aggacccaaa gcaatgactg atgtatttgt
    57841 cttttctttg aaataatcag ccaaaaaatc aacggcatgg agaggttctg cctgttttct
    57901 caagatcatc gggaaatttg tttcccctaa accatccata ccatgagtgt cttgtgcact
    57961 gatgaaaggt tgcttcaaag gtttttccat tccttgataa acaggaatgt ctaaacgatc
    58021 acagcgttcc aggcatttca gcacattctg cgtacctata tctgcaggga cattcccaca
    58081 aactacggta atagcaacta cttcgatatc aggggatttg acagctaatg ataaagctaa
    58141 agtgtcgtcg atccctggat cgcaatcaat aattactttt cgcattattt ccaatctcct
    58201 ttctttagag atacttagtt ttttattctg ggaagtttcc gaaccagtcc aagacgagcg
    58261 tcttgattat aatgcacgaa attagtatac tggcttttat tgcgaatgta aatgctctct
    58321 tacaagtaaa tctattgagg agaactttaa gtaaatgata caatgaaaaa gaaatggaat
    58381 ttacgtgtgc cgaaaataaa gaatcctatg gatcggtaca ttggataata gttagattat
    58441 agtttggcag aaagcggcgg gtgtacaatg gttatttttt atttcattct tattctgata
    58501 attataggga tcagttacca ttttctttct tatttttatg caaagaaatt agtaagagcg
    58561 ggagtacaga gaggatatga ctggtatgaa gacactggtc atcgtttgat ggaccctgct
    58621 gcagtcacgt cagtagaaaa aaaacgacaa aaattagaag aagagtgtga aagcttttgg
    58681 gagcagggaa cacccaaaac aatcaaaagt tatgacgggt tgaaactagc tggtcagatg
    58741 ttcatccatc caaataagca gaataaatgg gtcatttgcg tacatgatta ccgaagcaca
    58801 gggaaaagag acatgtctca tataggtaaa agatatgcag agaaaggatt caacgttctt
    58861 gtacctgatt tacgtgcgca cggagaaagt gaaggagaaa tcatcggaat gggatggcta
    58921 gatcggctag atttaattgc ctggatccaa ctcatcttag atgaacagcc gaatgcgtcc
    58981 attattttac acggcggatc aatgggagct tctacgatca tgatggcaag tggtgaaaaa
    59041 cttccatcag cggtcaaagg atttatttta gacagcggat atgtttctgt ttatgcagaa
    59101 ttccgatata tgttaagtaa aatcactgtc tttcctaaaa aaatggtcat gagatatgcg
    59161 aatcattatg cccagaaata cgccggttac tccttaaagc aagcatcagc tacgagacag
    59221 cttggcagca accatctgcc tttattggtc attcatggtg aaagagatca ttttgtccct
    59281 acagaagcag cgtacacgat tcaaaatgct acggcagggg ataaagcact gctgcttgtt
    59341 ccagaagcag aacacttgga agcatcgatg aaagatccga aaacctattg gacagtgatt
    59401 ttttcattta ttgaacaaag aataacgatg aattaatttt ctcttctttt ttgtaagtaa
    59461 ttcgaggata aataattgtt tatagatcag gagactgcta aaataaggta ggaataaaaa
    59521 aatatcagtt atttgaggag tgcggcatat ggaaatcaaa gaagaaaaaa atcgtcttgc
    59581 tttattcaat gatgaacaac aagagatcgg agaaatgaca tggtcagatg cgggcccaga
    59641 catcatgatc attgaccata catttgtaga tccagcatat cgtggtcaaa agttagctga
    59701 aaagctagtg tatactggag tagaattagc aagacgagac gggaaaaaga tcattccttt
    59761 atgcccttat gcaaaaaaag agtttgaaaa gaaaccagaa tatcaagacg tcttacgtca
    59821 gtcataagaa taacagctga aagttttgaa ctttcagctg ttattccatt tatatagagt
    59881 tgtcacggac ttttatctat gataaagtta aagagacgat atcttttggg aaggaggctg
    59941 cgatatgtat aatttaattt tgggaatcgt gatcgtttta tcgattgtta tggtcattgc
    60001 gattatgatg cagccaagca agcaaaatag tgcagcgagt gcatttaccg gcggtgcgga
    60061 tcaactattc ggcaaacaaa aagctcgcgg ctttgaagct gtcatgcagc gatcaactgc
    60121 agttatggga gctatctgga tgatcttatt atttgtccta gcatatttat cttcgaagta
    60181 gttgaataag cctctcgcaa aacggcgggc ggcttttttt attgcaaaga gaacgcttaa
    60241 gaagaagtta tgaaagaaac tatggtaaaa tgaggcgaaa gaggtgagac gagtgagaca
    60301 attaccaaaa cccctttatg caaaacatgg aaagcgagcg gttttgctgc ttcacgctta
    60361 ctcaggaagt ccaaacgatg ttcgaatgct cgcacgattt ttggaaaaag cagattatac
    60421 agtttatgcg ccgcttttta aaggacacgg aacaatggag ccatatgata ttttgcaaga
    60481 aaaagcagaa agctggtggg cagacactaa aaaagccatc cattttttac agtctgagca
    60541 gttttctgat attgccgtat taggattatc aatgggcggc atctttgctg tacgagcact
    60601 agaagaagaa tcagtgattg gcggcggttt tttctgttcg cctttgtctc ctgtaaaaac
    60661 aaacgtgcct gaaaactttg aaaaatacgt acgtcaagtg ctgaagacag caggtaaatc
    60721 agagaaagaa atcaatgaaa aagcagttgc ttaccgctcg ttagctgaac aacaattaat
    60781 ggatatacaa gatcaggcag caatcgtaga aagtaggttg tcagatatcc aacagccgat
    60841 atttttagct caagctggga aagatgaaat gatcgaccct aatggcgtat ttgaaactgc
    60901 acgcaagttg agccgtcagc gagtgacact tcgatggtat cctgaaagtg gtcatgtgat
    60961 tacagttggt acagcaagac gcgaattaga aaaagacgtc ttggaatttt tagaaaaatt
    61021 accttggaat gaggaataaa tatgacaaaa gaaacgataa aagagaaaat tctctttttc
    61081 atggaaagca gtcgcaaacg cagtttttct atggaagaaa tcgctgaagg tttagggttc
    61141 caaaaaagcg atgattttaa attactagta caaacgattg ctcaaatgga aagagaacag
    61201 agtatagttt tcaataaaaa aggaaaagtg aaacttcctt taaaaccaat cttgatcgaa
    61261 ggaacatttc gagcaaatga acgaggattt ggatttgtaa cgattgatcc cgaagaagat
    61321 gacatctata tcccaaaaga agcgactggt tttgcaatgg atggagacac cgtagcaatc
    61381 gatatcatga agcgagcaaa tacagatatg gatcgcggag cagaggggaa agtagtcgag
    61441 attcgagacc gggcgactac tcagttagca ggggaattcg ttgcttattc agaagaagaa
    61501 atacaagaaa cggatcttta cggcgttgtt atcccgaaag atagaaagct caaccaattt
    61561 aaagtatatg cagcagcaga gggagttcgt ccagttgacg gaagtattgt gctgatcgaa
    61621 ctgacccatt accctgaaaa agggtatgcg actagtttag aagggataat caagcaagtg
    61681 attggtcata agaatgaccc tggcatggat atcctctcaa ttgtcgtggc taatggtatc
    61741 cctacgaaat ttcctgatga tgtattgtct gaagcagatg aagttccaga tcaaatcagc
    61801 gaaaaggata taactggacg tagagattta agaaatcagc tcattgttac gattgatgga
    61861 gaagatgcaa aagatctaga tgatgcagtt acggttaaaa aattagataa cggtaattat
    61921 tttcttggag tacacattgc agatgtttct tactatgtga cacaaggcag tcaattggac
    61981 agagaagcat atgaaagagg gacaagtgta tatttgactg atcgagtgat tccgatgatc
    62041 ccgcaacgtt tatcgaatgg tatctgttca ctgaatcccc atgttccaag gctgaccatg
    62101 agttgcgaaa tggagattga tccaaatgga caagtagtct ctcatgatat tttcccaagt
    62161 gtgatccaaa caacagaacg gatgacctac acagctgtca atcaaatctt agaagatcaa
    62221 gacgaagaaa cgatgcaacg ctatgctcag ctcgtaccta tgttccaaga aatgggtgaa
    62281 ttgcatcgaa tccttgaaac gatgcgaatc aaacgtggcg cgatttcttt tgaggatcgc
    62341 gaagcaaaaa tccttgttga tgctgaagga cacccaaatg atatccagct aagaagcaga
    62401 ggaattggtg aacgcttgat cgaatctttc atgcttgcgg ccaatgaaac ggtagctgaa
    62461 cattataata aattaaatct gccgtttatt taccgaatcc atgaacagcc aaaagaagaa
    62521 aaaatgcagc gcttctttga ttttgcttca gctttgggca ttctggtccg aggaacgaaa
    62581 ggaacgatca caccaaaaga tttacagcat gtgatcgaag gagtagaaga caaaccagaa
    62641 gctgcagtta tcaatactat gttgctaaga agcatgcaac aagcacgcta ttcagaagac
    62701 aattttgggc attatggctt agcggcagat tactacacac actttacatc tcctattcga
    62761 cgctatcccg atctaatcgt ccatcgtctg atccgttctt acagtgaaga taaaagtgaa
    62821 agcaatcaaa gttcgtgggc tacggaactt cctgaaattg cagatcatag ttctaagatg
    62881 gaacgtcgtg cagtagaaac agaacgtgaa gtagattcca tgaagaaagc agaattcatg
    62941 atggacaaag tgggggaaga attcgatggg atcatcagct ctgttgtgaa attcggcctg
    63001 tttatcgaac tgccgaatac agtagaggga ttgatccata tcaatgaatt gaaacaggac
    63061 tacttccatt ttgttgaaaa tcatttagct cttgtaggtg aaagaactgg tctgactttt
    63121 aaaatcggac agaaagtgcg agtaaaagta gtcaaagctg atccagatga acgagcagta
    63181 gattttgaac ttgtttccgc agaagaggtt acagcgttgg aacggccaaa aacacgaaat
    63241 ggaaaatcag gaaaacgcga aggaaaacaa gcaggaaaac gcccagaaag aaatggcaag
    63301 aagaacaaac gatatagcga tagtaagcaa acgttcggta agaaaaaagg gaagaagcca
    63361 ttttacaaag gtgttaaaaa gaagaaaaag aaaaaataaa atgagttgga ggaaacagct
    63421 atgccaaaag gcgagggaaa attaattgca caaaacaaga aagctcgcca tgattattcg
    63481 atcatcgaca cgatggaagc agggatggtc ttgcaaggaa ccgagatcaa gtcgatacga
    63541 aacagccgga tcaatctaaa agatggattt attcgcgtcc gcaacggaga agctttcttg
    63601 cataatgttc atatcagtcc ttatgaacaa ggaaatattt ttaatcatga tccgttgcgc
    63661 acgagaaagt tattattaca caaaaaacaa atcatccggc ttgaaaatga attgaaaaat
    63721 actggaatca ctgttgttcc tttaaaagtc tatattcgta acggctatgc caaggtattg
    63781 attggtctgg cgaaagggaa aaaatcttat gataaacggg aagatttgaa acgaaaagat
    63841 atcgatcgac aaattgatcg aacattaaaa aatttctcta gataatttca ttatcctttt
    63901 tcgcatattc ggtgcttttg cttttactta atgataaaat ttgttagcat ttaatctgat
    63961 aaaacatgat tatcataggt tttttcttag atcaggagca ccgtgtataa agaaaaattc
    64021 gcttcgtttt tgactgaaat aaaaaagtgt ctaattttca tgaaaatgtt ttgaattttc
    64081 tgtttcatgg tggtatgata ccgtgtggtt agaaaccgat tggaaaaaac cagatacgca
    64141 catgaattga ttttatgtga atttcgggga aaagaggtga aatggattgg atgaacgctc
    64201 gctaacgttc cacattggac caatttggtt tgatggtaca gtttgtataa tggtgttact
    64261 gacctgtttg attgttttct tcttagctta cttttttaca agaaatctta agttgaaacc
    64321 aacaggcaaa caaaatgcga tagaatgggt cattgatttt agtcgaggaa tcgtaacaga
    64381 taatctgcct agaaaagaat tgagcaattt ccatttgttg gcatttacat tgtttttatt
    64441 tgtttttgtt gcaaacaaca tcggtttagt aacaaaaatc gttttaccta acgaaacaac
    64501 actttggaaa agcccgacag cagacccgtt tgttacttta actttggcat ttataatgat
    64561 cttacttact cacttatttg gggtgaaaaa gttaggcttc aaaggatatt tcgtaaattc
    64621 gttcttgaaa ccatatagtt ttatgtttcc gatgaaactg attgaagaat ttacaaactt
    64681 attaacactt gctttacgtc tttatgggaa tatttatgcg ggggaagttt tgctaacctt
    64741 gattgcaaaa atggtaataa gcttaggctg gttctcattg ccgttagcaa ttccattaga
    64801 gatggtctgg atcgcattct ccttgtttat cggaagcatc caagcatttg tcttcgttac
    64861 tttatctatg gtttatatga gccataagat tgaagtggaa gaataatatt cgttttaaaa
    64921 acaaactact atttatctaa tttaggagga aaacaattat gaattatatc gcagcagcaa
    64981 tcgcaatcat gggagcagca atcggagccg gttatggtaa cggtcaagtt atctctaaaa
    65041 caatcgaatc aatggctcgc caacctgaaa tgtctggtca attccgtaca acaatgttta
    65101 tcggggtagc tctagtcgaa gctgttccta tcttaggggt agttgtcgcc ttgattctag
    65161 tttttggtgt ttaattgaat cagtattaag gcagaaacaa tttggtttgt tactgctttt
    65221 ttacatgaaa actagaagta cttcaaagaa aggaaggctc gcctatgctg aatcaattgg
    65281 caattgcaga agttggcaat gcgatgttag gaaatgtcat cgttgtcagc ggctcatttt
    65341 tgatactact ggctctcttg aagcattttg cttggggacc aatcagcgat attttgaaaa
    65401 aacgtgaaga caagatcgcc aatgatttgg attctgcaga acaatctcgc atcaactcag
    65461 cgaaaatgga acaagaacgc gaacaacaat tgttagcctc tcgttctgat gcagctgata
    65521 tcatcaaaaa tgcgaaagaa agtggagaat taagccgcca aaatattttg aaggatgctc
    65581 aagaagaagc agctcgtcta aaaagtaaag cccaagctga tatcactgta gaacgtgatt
    65641 cagcgctgaa ctctgtaaaa gacgatgttg cagaactatc tcttcaaatc gcggaaaaaa
    65701 tcttgaacaa agaattatct ccagaaatgc acgaatcatt aatcaatcaa tatatcgaag
    65761 gtctaggttc ttcaaatgaa actagataaa tatacagtag gaagacgtta cgggaaagct
    65821 ttatttgaac tggcgatcga ttcaaataaa gcagaagaaa tttatcaaga tctcgtaagc
    65881 ttacgtcaaa tctatgaaca agtacctgga ttaggtaata tgttaagtga tgttcgtcta
    65941 gaacctcatg aaaaacgaga aatcatggac aagcttgtta gtggctatga tggaatcgtt
    66001 aaaaactttt tagaagttgt ttatgcgtat aaccgaatgg atgatctttt gttcatgatc
    66061 gaagaatatg aacgtcgata tgatgaacat caaggtttgc tgttgggaag tgtcacaaca
    66121 gcagttccac tttcagacga acaacgaata caattagaaa agaatgttgc aaaaacaatg
    66181 aactatcaat cagtcgaatt aaaacaaatc gttgattctt ctattattgg tggcgcgatt
    66241 gttgaagcta accatcgagt aatcgacgga agcattcgca cgcaattaga aaatatgcgt
    66301 aatcaattaa atagatagta caaagaggta ggtgaatcga atggccatca aagcagaaga
    66361 aattagtgcc ttgattaaag aacaaattga aaattatcaa aacgtgcttt cagtcgaaga
    66421 aatcggtacc gtaacttatg taggtgacgg gatcgctcgt gcacatggtt tagaaaacgc
    66481 gatgagtggt gagttgcttg aattttcaaa tggctcatac ggaatggcgc aaaacttaga
    66541 atcaaatgat gtaggtatca ttatccttgg cgatttcgag tccattcgtg aaggagataa
    66601 agtaaaacga acaggaaaga tcatggaagt tccagttgga gaggccttga ttggacgggt
    66661 agttaatccg ctaggtcagc caattgacgg actaggtgaa atcgttactg acaaagctcg
    66721 tccagtagaa gcaatggcac caggcgttat gcaacgtaaa tctgttaacg aaccaatgca
    66781 aacaggtcta aaagcaatcg acgctcttgt accaatcgga cgcggccaac gtgaattagt
    66841 tatcggtgac cgtaaaacag ggaaaacttc cattgcgatc gatacgatca tcaaccaaaa
    66901 aggtcaagat atgatctgta tctatgtagc aatcggacaa aaagattcta cagttcgtac
    66961 acaagttgaa acattgaaaa aatatggcgc aatggattac acaatcgttg tgaatgccgg
    67021 tgcgtctcaa ccagcgccgt tgctttatat cgcaccatat gctggtactg caatgggtga
    67081 agagttcatg tacaacggga aacatgtctt gatcattttt gatgatcttt caaaacaagc
    67141 tgttgcttat cgtgaacttt ccttgctgct tcgccgtcca ccaggtcgtg aagcttatcc
    67201 aggggatgtt ttctacttac actcacgctt attggaacgt gcagcaaaat taagtgatga
    67261 tttaggtggc ggttcaatga ctgccttacc atttgttgaa acacaagctg gagatatctc
    67321 tgcttatatc ccaacaaacg ttatttcgat cactgatgga caaatcttct tagaaagcga
    67381 cttgttctac gcaggtactc gtccagcggt tgatgccgga ctatctgtat ctcgtgtagg
    67441 gggttctgcg caaatcaaag caatgaaaaa agttgctggg actttacgtc tagacttagc
    67501 aagttaccgt gaattagaag catttacaca attcggttct gacttggatg cagcaacaca
    67561 agcaaaacta aatcgtggac gccgtacagt agaaatcttg aaacaaaaat tgcatgcacc
    67621 gctagctgtt gaaaaacaag tagtgatttt gtatgctttg acacatggat tcttagatag
    67681 tatccctgta gacagtattt tagacttcga acatgaatta ttcgaatatc tggataccaa
    67741 tcattcagat atctttgaaa caatccgtac aacaaaagat cttccagaag aagaagcgtt
    67801 gaacagtgcg attcaggaat ataaagacat gttcttagct acaaaaggaa ataattcctc
    67861 tacagaagat aaattgaaat ccattcaaaa tgcatagtga ggtgagatga atgggtgctt
    67921 cattaaatga aatcaagaca cgtatcgctt caacgaaaaa aacgagccaa atcacgcggg
    67981 caatgcagat ggtgtctgct tctaagttga caaaatctga agcttcttca cagaagtttc
    68041 aggtttatgc aacaaaagta agagaaatcg ttacgcattt gactgctact cagttaaatg
    68101 atattgcctc agaaaatcct agaggggata tcaattacaa cagcatgttg ctcagccgtc
    68161 ctgttaagaa aacgggttat atcgtcatca ctgcagatgg cggtttagtc ggtggatata
    68221 acagctcgat attgaaacaa acgatgtcta ttttagaaga agaccaccaa tcacccgatg
    68281 attacgtgat gatcgcaatt ggcggaactg gtgcagactt ctttaaagca cgcggtatca
    68341 atctagcata tgagctgcgc aatctatctg atcagccaag ctttgatgaa gtccgcaaaa
    68401 tcgtaaatat ggcaacaact atgtaccaaa acgaagtatt cgatgaatta tacgtatgtt
    68461 ataaccatca tatcaattcg ctaactagtc agtttcgtgt agaaaaaatg ctgccaatct
    68521 cagacttaga tccagaagaa gcaactactt tcgaacaaga atatattttt gaaccttcaa
    68581 aagaagaaat cttgtctcag ttattgcctc agtatgctga aagtttgatc tacggtgcga
    68641 tcgttgacgc caaaacagca gaacatgctg ctggtatgac agcaatgaaa acagcaactg
    68701 ataatgctgc taatatcatt gatgagctga cggtttctta caaccgtgca agacaagggg
    68761 ctattaccca agaaattacc gaaattgttg ccggagcttc ggcactagaa tagtattgag
    68821 attggaggaa aaaagatgag ttcaggcaag attgttcaag taatcggtcc cgttgtcgac
    68881 gtggaatttt ctttagacca atccttacca gatatcaaca acgcattagt tgtttataaa
    68941 aatgacgaaa ataaatcaaa agttgttctt gaagcagcgt tagaattagg ggacggagtg
    69001 atccggacca ttgcgatgga atcaacagat ggtttacaaa gaggaatgga agtcattgat
    69061 acaggcaaag caatctctgt tcctgtaggt aaagaaacat taggtcgcgt attcaacgta
    69121 ctaggagata cgatcgattt agaaacacct ttcccagaag atgcggaaag aagcgaaatt
    69181 cataaaaaag caccagcctt tgacgaatta agtacaagta cagaaatttt ggaaacaggg
    69241 atcaaagtta tcgatttgct tgccccatat ttaaaaggtg ggaaagtcgg actattcggt
    69301 ggtgccggtg ttggtaaaac cgtactgatc caagaactga tccataatat cgcccaagaa
    69361 catggtggta tttctgtatt taccggtgta ggtgaacgta ctcgtgaagg taatgacttg
    69421 tattatgaaa tgaaagattc aggagttatc gaaaaaacag ccatggtgtt cggacaaatg
    69481 aacgaaccac caggtgcacg tatgcgtgtt gctttgactg gtttgacgat tgcggaatat
    69541 ttccgtgatg tagaaggtca agatgtactg ttgtttatcg acaacatttt ccgtttcact
    69601 caagctggat ctgaagtatc agccttgtta ggacggatgc cttctgcggt tggttatcaa
    69661 ccaacattgg caacagaaat gggtcaattg caagaacgta tcacatctac gaaaaaaggt
    69721 tcgatcacat cgatccaagc aatctatgtg ccagccgatg actacactga cccggcgccg
    69781 gcaacagcat tcgcccattt ggatgcgaca accaacttgg aacgtaaatt aacagaacaa
    69841 gggatttatc cagcggtaga tccattggct tcttcttcaa gtgctttagc acctgaaatc
    69901 gtgggagaag aacattataa agtagcaaca gaagtccaac acgtattgca acgatatcgt
    69961 gaattacaag atatcattgc tatcttaggt atggatgaat tatccgatca agaaaaagtg
    70021 ttagtttctc gtgcccgcag agtccaattc ttcttgtcac aaaactttaa cgtggctgaa
    70081 caatttactg gtcttccagg ttcatatgtc cctgtagaag aaacagtgaa aggattccgc
    70141 gaaatcttgg aaggcaaata cgatgatctt cctgaagaag ctttccgaag tgttggccga
    70201 atcgaagacg tcgtagaaaa agcgaaaaca ctaggctact agaaaggggt gccccatgga
    70261 tcaatattta acagtaaatg tggtaactcc tgccggtttg gtctatgatc accatgcagc
    70321 tatcgtcgtt gcacgtacga cagctggtga gcttgggatt ttgcctaaac atgcaccaat
    70381 catcgttcca ttgacgattg atgaagttcg tgtaaaacga acagattcag atacccacgt
    70441 cgattggatc gctgttaatg gcggaatcat ggaagttcgt gataatgttg tttcaatcgt
    70501 tgctgatagt gcggaacgtg aacgtgatat cgatgtatct cgtgcggaac gtgcaaaaca
    70561 acgcgcagaa cgccaaatcg aagaagcaaa agcaaaacaa gatacgaacg aattgaaacg
    70621 ggcaactgtt gcgttgcacc gagcaatcaa tcgtatcaaa gtatctaagc actcataaaa
    70681 tgagatgcgg atgtttatcc gtgtttaaga acagtaaaaa gaggttatga cagaagtggt
    70741 cagcttcaag acataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatctc
    70801 agcttatttt tcgaagaagc tacttctgga acaccgtata tcggaggttg tgaaatcagc
    70861 gttttgtcct gagtattaga gaaacaaatg gaaaatagtt cctcatattt tgccgttttt
    70921 tgagctaata ccgagggtca gatgattgaa caccgtttat ttggaataag ggactgcgac
    70981 aagacttt
//
LOCUS       DACETN010000004        58323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DACETN010000004 DACETN010000000
VERSION     DACETN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.004"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..420
                     /locus_tag="IXF98_000254"
     CDS             <1..420
                     /locus_tag="IXF98_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1554594.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="IXF98_000255"
     CDS             complement(504..1226)
                     /locus_tag="IXF98_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1554595.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="IXF98_000256"
     CDS             complement(1216..1953)
                     /locus_tag="IXF98_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554596.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="IXF98_000257"
     CDS             complement(1965..2957)
                     /locus_tag="IXF98_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1554597.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="IXF98_000258"
     CDS             complement(2972..3715)
                     /locus_tag="IXF98_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554598.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="IXF98_000259"
     CDS             complement(3956..5278)
                     /locus_tag="IXF98_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1554599.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="IXF98_000260"
     CDS             complement(5265..6230)
                     /locus_tag="IXF98_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554600.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="IXF98_000261"
     CDS             complement(6227..7765)
                     /locus_tag="IXF98_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1554601.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="IXF98_000262"
     CDS             complement(8181..8468)
                     /locus_tag="IXF98_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554602.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="IXF98_000263"
     CDS             8602..9270
                     /locus_tag="IXF98_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1554603.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="IXF98_000264"
     CDS             complement(9561..10301)
                     /locus_tag="IXF98_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554604.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="IXF98_000265"
     CDS             complement(10431..11378)
                     /locus_tag="IXF98_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1554605.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="IXF98_000266"
     CDS             complement(11507..12271)
                     /locus_tag="IXF98_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1554606.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="IXF98_000267"
     CDS             complement(12275..12664)
                     /locus_tag="IXF98_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554607.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="IXF98_000268"
     CDS             12799..13341
                     /locus_tag="IXF98_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1554608.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="IXF98_000269"
     CDS             13489..14121
                     /locus_tag="IXF98_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554609.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="IXF98_000270"
     CDS             complement(14255..14380)
                     /locus_tag="IXF98_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554610.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="IXF98_000271"
     CDS             complement(14514..15407)
                     /locus_tag="IXF98_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1554611.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="IXF98_000272"
     CDS             complement(15589..16515)
                     /locus_tag="IXF98_000272"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1554612.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="IXF98_000273"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="IXF98_000273"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1554613.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="IXF98_000274"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="IXF98_000274"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1554614.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="IXF98_000275"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="IXF98_000275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1554615.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="IXF98_000276"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="IXF98_000276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1554616.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="IXF98_000277"
     CDS             complement(24786..25220)
                     /locus_tag="IXF98_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1554617.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="IXF98_000278"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="IXF98_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1554618.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="IXF98_000279"
     CDS             complement(26117..26992)
                     /locus_tag="IXF98_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1554619.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="IXF98_000280"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="IXF98_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1554620.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="IXF98_000281"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="IXF98_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1554621.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="IXF98_000282"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="IXF98_000282"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1554622.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="IXF98_000283"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="IXF98_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1554623.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="IXF98_000284"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="IXF98_000284"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1554624.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="IXF98_000285"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="IXF98_000285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1554625.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="IXF98_000286"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="IXF98_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1554626.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="IXF98_000287"
     CDS             complement(35443..36210)
                     /locus_tag="IXF98_000287"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1554627.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="IXF98_000288"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="IXF98_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1554628.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="IXF98_000289"
     CDS             37323..37721
                     /locus_tag="IXF98_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1554629.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="IXF98_000290"
     CDS             37958..38500
                     /locus_tag="IXF98_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1554630.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="IXF98_000291"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="IXF98_000291"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1554631.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="IXF98_000292"
     CDS             complement(39286..39513)
                     /locus_tag="IXF98_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1554632.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="IXF98_000293"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="IXF98_000293"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1554633.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="IXF98_000294"
     CDS             complement(40048..40761)
                     /locus_tag="IXF98_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1554634.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="IXF98_000295"
     CDS             complement(40764..41660)
                     /locus_tag="IXF98_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1554635.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="IXF98_000296"
     CDS             complement(41818..42660)
                     /locus_tag="IXF98_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1554636.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="IXF98_000297"
     CDS             42792..43445
                     /locus_tag="IXF98_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1554637.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="IXF98_000298"
     CDS             complement(43508..44035)
                     /locus_tag="IXF98_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1554638.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="IXF98_000299"
     CDS             complement(44055..45002)
                     /locus_tag="IXF98_000299"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1554639.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="IXF98_000300"
     CDS             complement(45014..46900)
                     /locus_tag="IXF98_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1554640.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="IXF98_000301"
     CDS             complement(47060..49138)
                     /locus_tag="IXF98_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1554641.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="IXF98_000302"
     CDS             complement(49151..49426)
                     /locus_tag="IXF98_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554642.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="IXF98_000303"
     CDS             complement(49788..50243)
                     /locus_tag="IXF98_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554643.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="IXF98_000304"
     CDS             complement(50397..51605)
                     /locus_tag="IXF98_000304"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1554644.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="IXF98_000305"
     CDS             complement(51717..52049)
                     /locus_tag="IXF98_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1554645.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="IXF98_000306"
     CDS             52197..53072
                     /locus_tag="IXF98_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1554646.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="IXF98_000307"
     CDS             complement(53154..53735)
                     /locus_tag="IXF98_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1554647.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="IXF98_000308"
     CDS             complement(53741..55000)
                     /locus_tag="IXF98_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1554648.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="IXF98_000309"
     CDS             complement(55002..56048)
                     /locus_tag="IXF98_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554649.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="IXF98_000310"
     CDS             complement(56283..56558)
                     /locus_tag="IXF98_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1554650.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /locus_tag="IXF98_000311"
     CDS             complement(56801..58111)
                     /locus_tag="IXF98_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1554651.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT        16051 a        12217 c         9966 g        20089 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgttt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaaggctct atactttttg
    58261 atgttggtgg atgacaccag catctttttt attttccaaa acatatagaa aagacacctt
    58321 gaa
//
LOCUS       DACETN010000005        56296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DACETN010000005 DACETN010000000
VERSION     DACETN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.005"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..189
                     /locus_tag="IXF98_000312"
     CDS             <1..189
                     /locus_tag="IXF98_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1554652.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IXF98_000313"
     CDS             complement(1055..1585)
                     /locus_tag="IXF98_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1554653.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IXF98_000314"
     CDS             complement(1582..2199)
                     /locus_tag="IXF98_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1554654.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IXF98_000315"
     CDS             complement(2781..3002)
                     /locus_tag="IXF98_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1554655.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IXF98_000316"
     CDS             complement(3180..4712)
                     /locus_tag="IXF98_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1554656.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IXF98_000317"
     CDS             complement(4848..5933)
                     /locus_tag="IXF98_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1554657.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IXF98_000318"
     CDS             complement(5948..6667)
                     /locus_tag="IXF98_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1554658.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IXF98_000319"
     CDS             complement(6664..7974)
                     /locus_tag="IXF98_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1554659.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IXF98_000320"
     CDS             complement(8001..8591)
                     /locus_tag="IXF98_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1554660.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IXF98_000321"
     CDS             complement(8909..9970)
                     /locus_tag="IXF98_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1554661.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IXF98_000322"
     CDS             complement(10538..11605)
                     /locus_tag="IXF98_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1554662.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IXF98_000323"
     CDS             complement(11625..12104)
                     /locus_tag="IXF98_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1554663.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IXF98_000324"
     CDS             complement(12121..12582)
                     /locus_tag="IXF98_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1554664.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IXF98_000325"
     CDS             complement(12596..13846)
                     /locus_tag="IXF98_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1554665.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IXF98_000326"
     CDS             complement(14224..15675)
                     /locus_tag="IXF98_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1554666.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IXF98_000327"
     CDS             complement(15996..16760)
                     /locus_tag="IXF98_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1554667.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IXF98_000328"
     CDS             complement(16788..17486)
                     /locus_tag="IXF98_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1554668.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IXF98_000329"
     CDS             complement(17498..18277)
                     /locus_tag="IXF98_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1554669.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IXF98_000330"
     CDS             complement(18293..19237)
                     /locus_tag="IXF98_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1554670.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IXF98_000331"
     CDS             complement(19283..20062)
                     /locus_tag="IXF98_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554671.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IXF98_000332"
     CDS             complement(20442..22517)
                     /locus_tag="IXF98_000332"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1554672.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IXF98_000333"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IXF98_000333"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1554673.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IXF98_000334"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IXF98_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1554674.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IXF98_000335"
     CDS             complement(24783..25682)
                     /locus_tag="IXF98_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1554675.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IXF98_000336"
     CDS             complement(25697..26098)
                     /locus_tag="IXF98_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1554676.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IXF98_000337"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IXF98_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1554677.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IXF98_000338"
     CDS             complement(26581..28773)
                     /locus_tag="IXF98_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1554678.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IXF98_000339"
     CDS             complement(28797..29768)
                     /locus_tag="IXF98_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1554679.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IXF98_000340"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IXF98_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1554680.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32008..32454)
                     /locus_tag="IXF98_000341"
     CDS             complement(32008..32454)
                     /locus_tag="IXF98_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1554681.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32481..32657)
                     /locus_tag="IXF98_000342"
     CDS             complement(32481..32657)
                     /locus_tag="IXF98_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1554682.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32819..33247)
                     /locus_tag="IXF98_000343"
     CDS             complement(32819..33247)
                     /locus_tag="IXF98_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1554683.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33339..34199
                     /locus_tag="IXF98_000344"
     CDS             33339..34199
                     /locus_tag="IXF98_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1554684.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34293..34586)
                     /locus_tag="IXF98_000345"
     CDS             complement(34293..34586)
                     /locus_tag="IXF98_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1554685.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34659..36386)
                     /locus_tag="IXF98_000346"
     CDS             complement(34659..36386)
                     /locus_tag="IXF98_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1554686.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IXF98_000347"
     CDS             complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IXF98_000347"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1554687.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IXF98_000348"
     CDS             complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IXF98_000348"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1554688.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39103..40506)
                     /locus_tag="IXF98_000349"
     CDS             complement(39103..40506)
                     /locus_tag="IXF98_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1554689.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40736..41638
                     /locus_tag="IXF98_000350"
     CDS             40736..41638
                     /locus_tag="IXF98_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1554690.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41679..43565)
                     /locus_tag="IXF98_000351"
     CDS             complement(41679..43565)
                     /locus_tag="IXF98_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554691.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43662..44514)
                     /locus_tag="IXF98_000352"
                     /pseudo
     CDS             complement(43662..44514)
                     /locus_tag="IXF98_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44849..46633)
                     /locus_tag="IXF98_000353"
     CDS             complement(44849..46633)
                     /locus_tag="IXF98_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554692.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46633..48384)
                     /locus_tag="IXF98_000354"
     CDS             complement(46633..48384)
                     /locus_tag="IXF98_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1554693.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48526..48750)
                     /locus_tag="IXF98_000355"
     CDS             complement(48526..48750)
                     /locus_tag="IXF98_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1554694.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49001..50587
                     /locus_tag="IXF98_000356"
     CDS             49001..50587
                     /locus_tag="IXF98_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1554695.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50653..52428)
                     /locus_tag="IXF98_000357"
     CDS             complement(50653..52428)
                     /locus_tag="IXF98_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1554696.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52639..53541)
                     /locus_tag="IXF98_000358"
     CDS             complement(52639..53541)
                     /locus_tag="IXF98_000358"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1554697.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53820..56165
                     /locus_tag="IXF98_000359"
     CDS             53820..56165
                     /locus_tag="IXF98_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1554698.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16684 a        11714 c         9117 g        18781 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt
    32041 tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc
    32101 aaattctttc attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat
    32161 ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac
    32221 tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg
    32281 tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt
    32341 aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc
    32401 tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc
    32461 aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc
    32521 ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc
    32581 gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga
    32641 acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt
    32701 attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg
    32761 accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt
    32821 attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac
    32881 cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca
    32941 ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact
    33001 tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat
    33061 ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt
    33121 ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt
    33181 tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcaatc aatgtctgtt
    33241 ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt
    33301 ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa
    33361 tcattacttt ttttgtcatc tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag
    33421 ccactatggc ccaatatcct accgttgagt tcaatttatt ccgcagaaca tttgtgacag
    33481 atgagaaaac attgatgcag gcactgcaag atgcactaaa agaaaaagcc cttgtcctac
    33541 acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat
    33601 tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca
    33661 agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca
    33721 aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag
    33781 cagatgtcgt tttgctaggc gtatccagaa cttccaaaac gcctttgagc ttgtttttag
    33841 ctaacaaaaa tttaaaggtt gccaatctac ctttgatccc acaagcacat attcccaaac
    33901 agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa
    33961 acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg
    34021 atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct
    34081 gcgtagtaat caatgtcgct tctttatcta tcgaagaaac agcttcactt attttgaatg
    34141 ctttagacct agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac
    34201 agtttttctt gtctttttct taaaaaaact ggactgtgtc agcgaagaag attcgttgat
    34261 acagtccagt ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagcgtc
    34321 tggttcaaaa tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca
    34381 cgatgggact tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat
    34441 tgttccattt ctactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac
    34501 agcagaattt tgagacgagt ggggtcactc aacattttga atagttggct gactttctct
    34561 acttttgttt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    34621 ctaaagttta ccactttttc catcagtctc aagttttttt attcttctgt catttttttg
    34681 ataatcgctt cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca
    34741 ccgatataaa atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt
    34801 tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct
    34861 tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca
    34921 aattctgtcg gtgcttcttc acggaatttt tccatcaaag cagagatttt tgcagagcct
    34981 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa
    35041 atcgtttgca agccgtcata caatgttttg ccttgttttt tatagtaagc tgctacttct
    35101 gctaataaga gcaatgcttg aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa
    35161 ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga
    35221 attttttcag caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt
    35281 tccgcaattg ctgtaggcaa ttcgctagaa acgattgatt tcaataccgc tgcattttca
    35341 ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt
    35401 tggtttcctg tcaatacttc ataggagcca tttggcaaac gaacggctgc gcctaaacgg
    35461 tctgcatcag gatccgttgc aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc
    35521 gcatactcga aagcagagtg ttcttctgga ttcggtgatt tgactgtagt gaaatccggg
    35581 tctgcgacag cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca
    35641 cgttcgccca acatttttcc tgttccgtga agtggtgtgt acacaagctt caaatcttta
    35701 cccattgttt ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg
    35761 gcatccactt cttctccgat gatcgtgatc aatccgctat gttcagcttc gtcgtcagat
    35821 aatacttcaa cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
    35881 tctgcaggag gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct
    35941 ggattgtggg atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa
    36001 gaaagctctg gtgttggacg taaactttcg aatacataag aagggatatt gtgatgtgct
    36061 aatgttttgg ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata
    36121 gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct
    36181 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggtcctaa gattccccgc
    36241 atgcctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
    36301 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
    36361 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
    36421 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
    36481 agaaaagatt cttttatcaa gcacggttac aagtattcgc ataaatcgga ttcagtagat
    36541 ttcacgcaaa aaaaagagta aaagaacaat tgaaaccgtt ctttcactct ttttaaactt
    36601 atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca gcaatcccac
    36661 catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    36721 aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg
    36781 tcaacccagc ttctctaaat ggtgcagtta atggatccaa tcctacatag atgaatactc
    36841 cgtctgctgc caaatcaaac tcattgccag ttttgacatt acgtgctttc acacctgtca
    36901 ctaccatttc atttccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    36961 cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    37021 cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc
    37081 ctcctccgat cactactaga cgtttgttac ggaaaaatgc accatcacaa actgcacagt
    37141 aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    37201 agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt
    37261 tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc
    37321 ttgaaacacc ttcatacatc ttataagcta gatcaggacc catgattgaa tcgaaaccag
    37381 gatagttttc gatttctgcc gtattgttca tttggccgcc aggagcgcct tgttcgatca
    37441 tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggccctg
    37501 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    37561 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    37621 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caatcgttcg
    37681 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg
    37741 aacgggctaa tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt
    37801 tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta
    37861 atagatcgac tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat
    37921 agtcgattcg ataatcatct tccacataac cattctcatc gggcgtatcg actgcaccta
    37981 agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta
    38041 ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga tatggatttt
    38101 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa
    38161 ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg
    38221 catccccagg ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag
    38281 cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgactttc
    38341 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    38401 tacagcctac tttgttctcc ggatctactt catgagcgat cttcgttgcg atggcacttg
    38461 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    38521 aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca
    38581 accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa
    38641 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat
    38701 caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat
    38761 cctcataaaa tttcaaccca gattcatttg gctctttttc atcgcccatt ggaaaaatac
    38821 gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat
    38881 cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat
    38941 caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca
    39001 caacatctac gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg
    39061 ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac
    39121 atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt
    39181 taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc
    39241 atccatgatc cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg
    39301 caaagctgtt ccataatcac cagtcacggc tgcttcgatc gtcagcaatt ccatattctt
    39361 cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc
    39421 gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac
    39481 agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc
    39541 aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg
    39601 tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac
    39661 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    39721 ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    39781 tttgaaaaac gggatgtcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg
    39841 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    39901 gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg
    39961 ttctgtcatc atcgccatta ctggcacatt gcataatccg atcactttat cccatttgcc
    40021 atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattaa tcagccaagc
    40081 atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa
    40141 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    40201 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    40261 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg
    40321 agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc
    40381 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    40441 ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    40501 tgccatgttc tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa
    40561 acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt cttctggatt
    40621 tttgtaaata tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca
    40681 aatcattttc aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt
    40741 attacgtgaa aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt
    40801 tctcttaagc gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg
    40861 ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt
    40921 cagttttcgt aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt
    40981 agatgcgaat cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc
    41041 ttctttagaa cagatgacga ttcaagatac actttctctc attcatcatg accaactgca
    41101 aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa
    41161 tactttgatt tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat
    41221 tgtcaccact tttggtgaat cgttctatga agcgtacaat tgcacaagtg atatgtgtgg
    41281 gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa
    41341 acaaaatcat gtgccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat
    41401 gagtacagcc gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt
    41461 tacgatcaat acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga
    41521 aaattatcaa cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc
    41581 tatcagctca gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa
    41641 agaatagtta agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt
    41701 gcatcgtctg gcccgaccgt ttccccttga ttttccaaaa tatccaaata atccatgtaa
    41761 ttgatgacaa tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg
    41821 aagcgaagca acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa
    41881 tatttccctt ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct
    41941 tcagaaacca tcccgattgc atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa
    42001 ggggcacgta cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt
    42061 gcaaaagctg catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt
    42121 acaatttctt tacctgatct tttctgtcta tcatctgttt catctgcttc ctccttccaa
    42181 aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat caatacatta
    42241 atcacatctg ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctagt
    42301 ccaccttgat tccaagaagt taaattcatt aacccactat acgcgccccc taacgcacca
    42361 ccgacacagg taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc
    42421 tctgtaatcc ccacaatacc agaaacaatg gctggtattg caattgcttt gtcttttgga
    42481 tttctaagct taaagtagag tgcggtaagt gctgcagttt gtccaaaaga cactactgca
    42541 acaggtgtca taatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg
    42601 attgcccaat ggagaccaaa catcaccaga atttgccaga agaagcctac gattgctcca
    42661 taaataactg gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg
    42721 aatgtgaata ctggtccaac gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag
    42781 aatggaacga agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt
    42841 tggctagcta acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca
    42901 atccagggaa tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat
    42961 atggtcgtta gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc
    43021 attaatgctg cgccaagcaa catccctacg aattcattta ctttaaattt acgggcagct
    43081 gttaaaccga taataacagg cataaattgg aatacagaat caccgatatt atcaaacata
    43141 acaaatgtgc cagaaccgcg gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca
    43201 cgaatcatcc cgtcagcagc taggatagca aggaatggtt ggaaacagcc cgatattcca
    43261 tcaataagca tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg
    43321 tttcctgctt ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttga
    43381 tactgccctc ccgctcgcat taccgttaca ataccgtcca tatttttcaa gatctcatca
    43441 tttgcttttt cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca
    43501 ttgatatttt ccttcccgcc gacattttct acgatttttc tcgctaattc ttggtatttt
    43561 cccatttcta ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc
    43621 ttatttcata cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca
    43681 tgcactaaac gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag
    43741 ttgaacgtct cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat
    43801 tttttttgaa taacctgaaa caattcctca gaaacttctc ccacttctga tttttctcca
    43861 tctaggacgc gtgtcagaaa aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa
    43921 tcttcatcaa atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt
    43981 atagtgaaca ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc
    44041 aatgcgataa atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc
    44101 ttcactgttt tttttcccga tggcatactc tttaggaaat agttgtttga tatcatacag
    44161 caatacattt ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc
    44221 cgtcaatgca atatacacag aatcattcaa ctggacttgt agagcctctt ttgtatattc
    44281 aatgatttca caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa
    44341 ctgttgtgaa agcccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat
    44401 cgggtcaccc actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt
    44461 tctttcatcc ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgttttg
    44521 ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc
    44581 catggaacca tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg
    44641 tatcgttttc tttatattat cgaaagcaaa cgcttttgtc aacgctttct tagtggttat
    44701 tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc
    44761 tcacagatga agaatctgcg aagctctgtt ttattttcac tctagcgctt agggcaaaat
    44821 gctactatgc tactgtcaca acctcttttt acacaccact gctttgcagt ttgtacatat
    44881 ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgctct actatatgac
    44941 ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga tgggcaatcg
    45001 ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc ttggatcaat ccttcggttt
    45061 cagtatcgat atttgctgtt gcttcatcca atactagtat tttaggatct gtcacgattg
    45121 ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata gctggcaccg tgttcgatca
    45181 cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga acaaactcag
    45241 ctgcatggcg aatctgttcg tctgtgatag ctggattcat catccggata tttcctgcaa
    45301 tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta cgtaactctt
    45361 ccattggata ttctcgaatg tcatgatcat caatcaagat ttggccttca taaaattcgt
    45421 aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc cccactaatg
    45481 caacggtctc acccggatta gcgacaaaag agatattgtt caatacattg tgctctccat
    45541 cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct tgatctgctc
    45601 caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata cgactaccgg
    45661 caaccatccc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa
    45721 attcctggac ataggtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg
    45781 catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatagaga aaattgatga
    45841 ttggacttaa cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt
    45901 tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc tggataatac
    45961 gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt tcccgcatat
    46021 ttcggtaaat acgagaactg aatttctggt aataccagat gacgattcct aaaatcggca
    46081 agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca acgaaagaag
    46141 aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg
    46201 tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac
    46261 gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga taggtcttca
    46321 atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg actatgactc
    46381 caaacaagta cacaccagca aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca
    46441 gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc acagacaggg
    46501 caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg aacttcatca
    46561 gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat tccgattgat
    46621 aattttcatc catcaggcct cgcctccttc tatcttcgct tctaattgct gtttttccca
    46681 cattcgttca taccagccac ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat
    46741 tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta aacggtgagc
    46801 tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga tagcttcttc
    46861 tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag gatttttgat
    46921 caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca gataaagaga ctccacgttc
    46981 tccaactaag gtatcataac cttttggcaa ttccttgatt tcattgttga taaatgctaa
    47041 agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac ggatattatc
    47101 tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca taaccgattg atcctaataa
    47161 agcattcaat gaatattctt tgatcgaacg tccgccaaat tggattttcc cttgataatc
    47221 atcgtattca cgcatcagta atttgatgat tgtcgttttt cccgcaccag ttttcccaac
    47281 aatacctaat gtttctcctt cttggatcgc aaatttgatt tgttctaatg ctggtgattc
    47341 ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg ccggggtctg
    47401 cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc
    47461 ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca
    47521 aacaagcata ccgatatagc taataaatga gaccatctgc ccgattgaga tcgtgccttc
    47581 caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga taatcaaggt
    47641 aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa taattgcatc
    47701 ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg ttttaatgac
    47761 tttgatccca gtgatacttt cttgtgtctt atcattgata ctagaaaaag cttcttgcga
    47821 atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg
    47881 cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat
    47941 cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag attccagctc ctgctacatt
    48001 ctggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc gatatttttg
    48061 gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc
    48121 caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa gatattgtaa
    48181 gaaagctgca aggatcaata ataagatcca ccaggaaatg atctttattg ataaattctt
    48241 atcggcgatc tcatcaatga tgatcccaat cacttttgga ggaactaact gtaccaaggc
    48301 cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgac gaaaaaacca
    48361 acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag agcataggat
    48421 tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta cgaaataaaa
    48481 aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt ttttcccttg
    48541 gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt gccccattga
    48601 catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta aatagtcttt
    48661 catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag caataatagc
    48721 aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt tccgaattgc
    48781 tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc tttagtttca
    48841 tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac cagcaagaca
    48901 caccacgaaa aaaaattagg cataccgaat ctttttcttt aattttttct ttctgaggtg
    48961 tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc aatccaacaa
    49021 acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag ctgaagtaaa
    49081 tggaacagtg gatgtaccaa agggaaaaag tttttttaaa acacttcttg cttactctgg
    49141 accaggtgca ttagtagcag ttggttatat ggatccaggt aactggtcca cttcgatcac
    49201 tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta gtctaattgc
    49261 tatgctttta cagtatatgg cagcaaaatt aggaatcgtg acccaaatgg atttggccca
    49321 agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct tgactgaatt
    49381 agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc tctacttact
    49441 ttttgatatt ccattggtga ttgccgtctt tattactgta ttagatgttt tcgttctctt
    49501 actactgaca aaagtcgggt ttcgtaagat cgaagcactg gttgtctgct taattttcgt
    49561 cattttattc gtttttgtct atcaagttgg cttgtctaac ccagattggc acagtttatt
    49621 tgctggcttt atccctaaca gtcatacatt ctcagactca ccggtcatcg gcggcgaaac
    49681 tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata acttatatct
    49741 tcattcagca gtttcacaaa ctcggaagat cgatcaccaa aacgaagaag atgtagcaca
    49801 agctgtacgt ttttctactt gggattctaa tatccagttg tcaatggctt ttctagtcaa
    49861 tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc
    49921 cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg gtattttgat
    49981 tgctgtagca aaatctggta tcttgtccac tttatttgct gtagcacttc ttgcttctgg
    50041 ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag gattcatcca
    50101 tatgcgtatc ccgatttggc taagaagact ggtcacacga ttgctttctg tgattcctgt
    50161 attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacatg ccgctttgaa
    50221 taacttaatg aatgaatccc aggtgttctt ggcttttgcg ttgccatttt cgatgattcc
    50281 tttactgatg atgaccaaca gccaagcgga gatgggtagc cgatttaaaa attcattcat
    50341 cgtaaaactt cttggctggt tctctgtgat tagtctaact tacttgaact tacgaggatt
    50401 accaggacag atcgaaagtt tcttcggcga gcaagccact tccgctcaac tagcattggc
    50461 tgatcacatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat ggacgattgg
    50521 tgatttatat aaaggaaaca aaaaatatga acagcttttg caggaaacgg tagaagaaat
    50581 cgaataaaaa ctgatttttc cacacaaaga cccttgatag ttaggttaaa aatctagcta
    50641 ccaagggtct ttttatttag aagataaagc ttttttcaga tttaatttac tgaaaactag
    50701 catgcttgtt actacgatga attgcgttaa tggcacgcct gcaccagtgt agacacttaa
    50761 aaaatagtcc tttttttctg gcgcataaga acaaaaccat gagacaccga aaagaaaaac
    50821 caattgtgtc agtattaatc cggtgaccac aatcaaagag gaactataat aggcacgatc
    50881 agcttttgca aatacggttc ctagtatcat aagtaatatc cctgctattg aagcaatcag
    50941 cacgggaata gccagaactt ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt
    51001 tgttaattgg aaattaggtg ttctcggttc attactatcg atccaattct ccattagcgg
    51061 aaggtttaat aattcttgag cctgtttgac ttgttcttgc gttccgaaac tataatttcc
    51121 tccttgatgg aaatctttat atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa
    51181 tacgattggc agatcactca tcgtctttcc atctcctgat cccatcagat agaactcttt
    51241 tttctttttg atggttccct tgtgataggc ttcttctaat tcttggttga cttttttcca
    51301 aaaatcattg gtttcttttg cattgttttg gtaataacca gattcgctta ccacatctcg
    51361 caatgcccaa aagatgatat ctccgggtaa ttcttttact tttccacctt gagtccacgc
    51421 gtgagtcgta tataattcgt caatacgggt agaaacagta tggaatgtag gagatgcggc
    51481 taatgcttta tttagtgcag catgtgagac ccatatctca ctgttttctt tattcaagtt
    51541 cgtttcatca tctattcgga tcagttgttt catcaaacgt ccaaattctc cactggaacg
    51601 gtcattgatg gttgcaatac cgtatgttgt ctcattttta tattcgatcc actgatagac
    51661 gatggaaaag ccaactatag gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga
    51721 gagaagaaag agtcgttttt ttaacttctt tacttctttg aaagaaaagt cttgccctag
    51781 caagatctgg acgaaactga tacccaaagc tactacagta aaaggaagaa gccagataga
    51841 atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa agccctaaaa accatggcag
    51901 caattttctg ctagaaccgt atttgcggta ataaatagct aataaacaag cgataatgat
    51961 ctcaacagcc ggaatcacga gggcattacg atatgttcga gtggaatatt catgtgtaaa
    52021 actaatcgga gaaaacaaaa aataaaaata gatacttaaa agaagatact tgttcttcac
    52081 aatcggttta aacgcgagtg caaccaatat actggcaaaa atactgaaca tgccgaacaa
    52141 aactgcataa gaaagatgca gtttatgtgc aagtactaaa aataagctgt aggagattcc
    52201 tttggaaagt gttgtagtcg tatactcccc tagccattta ccatccaaaa tattttgagc
    52261 atagtgaatc agcagctgat catcataggt cgccgacata tctgttccat agattgcttt
    52321 actatataaa tacaaccgaa taattgtaaa taatacggct atgccaagaa taaagtatgt
    52381 atgtttcttt tttagttggt cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt
    52441 tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt tttacttttc tatcagtata
    52501 ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa
    52561 aagcactcct ttgtttctta aacaaaaaag gacaaggcag actgcccaat ccttttttgt
    52621 tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct aataactcag ggaacacttc
    52681 gggatcgaat gcttgcgctt tgaacatgga aatttctttt ccatatggtg cgtaagcttt
    52741 ctttttatct ttaccgacat atggggtttc taatattttc gaaatgttgt caaacattgg
    52801 atcatggacg attttgttca atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg
    52861 ccgatcttta tgagaacctt gtggattttt agaatcattg atatgcagta ctttaagccg
    52921 atctaatccg atgaccttat caaactcttc cattacacct gaaaaatctt cttttatatt
    52981 atatccggca tcattcgtat gacatgtatc aaaagtaacg gataattttt cattcaattt
    53041 tactccatcg atgatttgcg ctaattcctc aaatgttcga ccgatttccg tacctttccc
    53101 tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc
    53161 ctcaataatt ctagcaatcc cagcttctgc ccctgctcct acatgagcgc ctgggtgaag
    53221 ggtgatttga gtagcaccta aagcttccgc acggacaatc tcttctctca gaaattgtac
    53281 agcaaatgga aacatttctg gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg
    53341 aacgacgata tttgttagtc cgttttctgc cataaatgct tttccggcat caatattcat
    53401 ctcttcgata gcttttcttc tagtattttg aggagcgcct gtatagatca taaaggtcga
    53461 agatccgtaa ctggctgctt cttctgccgc gcctaacagc attttctttc cgctcatgct
    53521 gacatgtgag cctaataaca tggatggatt cctccttgaa aggcaattgt ttcgaacaat
    53581 tcctgtatat tgataagatg atgtacaaag aaaatggttt gtttttcttt gccctgttct
    53641 ccatagtaac tgaaactttc cctaaagaaa agcttaagaa ctctttcata aaaaagatca
    53701 tttcctcacg ttttatctca ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt
    53761 tttttgtcct attatttgct ataattagcg aaataaaacc attagttgag gtgacccttt
    53821 tgccacgcaa aaatactcga aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc
    53881 ctaagattat ttttagagtg tttcaatcat tgactgtttt tattactgtt cttcttttgt
    53941 tgttcgcagc tctaggtatc ggaataggtg ctggctactt tgcctatctt gtcgaagaca
    54001 cacaattgcc gactaaaaaa gaactgcaga cagaactagg aaacattacg gaaacgtcaa
    54061 agattgttta tgcagataat acagagatat caaaaatcca gacagatctg atgcggacga
    54121 ctatttcatc tgataagatt tcgcctttat tgaaaaccgc gattatcagt acagaagatg
    54181 aatacttcga taaacatcaa ggttatgtcc ctaaagctgt tttacgagca ctagtttcag
    54241 aagcgactgg catcggctcg tctggaggat ccactttgac acagcaatta gtcaaacaac
    54301 agatcctaac cgatgaaaca acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac
    54361 aagtagaaaa acacttttca aaaaatgaga tcattactac gtatttgaat gtctcaccat
    54421 ttggtcggaa taataagggg caaaatattg ctggagtcca ggaagctgct agaggaatct
    54481 ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc ttatatcgct ggtttgcctc
    54541 aaagccctat cacgtatagt ccatacacga atacaggcgc gctaaaagaa gatctttctg
    54601 ccggtcttgc cagaaaagat tttgttttat tcagtatgta ccgtgaagga caaattacga
    54661 aagaacaata cgaagaagcg aaagcctatg atcttacaaa agactttctc ccacaacaaa
    54721 tcgcggaaca gactgacaga gaattcttgt attataccgt gatgaacgaa gcaacaagga
    54781 ttattgcaca acaattggca gaaaaagata atgctgatat gtcagattca tcggtcagtg
    54841 aagcttatta tcaaaaagca cagcaaacga tccaaaacaa aggatatacg atccattcga
    54901 cgatcgataa agatatctat gctgcattac aaaccggcgt agaaaattat ggctacatgt
    54961 tagatgatgg tgctggtagc caagtggaga ctggaaatgt attgatggat aacagaaccg
    55021 gacgtatcta tggcttcgtg ggtggtcgga attacagcca gaatcaaaat aaccacgcct
    55081 ttgatacaga acgtcaagct ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa
    55141 tcgatatggg acttgtcgga tcagaatccc gtgtatcaga ttatgcaacg acttggcaag
    55201 aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc
    55261 aaaccgtccg tgagtcactg gaatggtcca acaatatccc agcttatcac ttatatcaag
    55321 atgtgttgaa ttccggtgga tcgaaacaat acgcttacga gcactacttg gcgaagatga
    55381 actacccagc aaatgataat tggggcgttg aatcggctcc tttagggaca gtagatgtaa
    55441 cgacgcttca acagacaaat ggattccagg ccttagcaaa tggtggagtc tacgaagaag
    55501 gctatatcat tgattccatc acagataatg caggtaatgt gatttataaa catgaaagta
    55561 attctgtccg tatttattcc gaagcaacgg catcgatcat gaatgacatg atgcgtagtg
    55621 tgatcaatga aaaaatcact acaccattca aagatgtcat ctcaagctta aacggaaatc
    55681 taggaaatgc agattgggtc ggaaaaactg gttctaccaa tgaataccga gattcatggc
    55741 tgatcgtctc aactccatct atcactatca gtagttgggc aggacacgat gacaatactg
    55801 gaatggacag ccaagcaaga atacgttcgg ctaattatct tgccaatcta atcaatcaag
    55861 cttaccaagc aaaccctgag attttcggca ctgatgagaa atttgactta tcatccgatg
    55921 tgatgaagaa aaaagtatct gggttcacag gacaaacagc tggaaaagtt actgtcaata
    55981 aaaaatccat ccaaacgcca ggaaaaacag tggagtcact ttgggcaaaa aatggtccga
    56041 agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga aaactataca gattactgga
    56101 acaccgcttc agcttatgct agagcgaatc cagagaccaa agaatcagac gataaagatg
    56161 attgatctaa tagattaatc agaattaaag ggagctgcga caaaaatctt gtcacagctc
    56221 cctttttccg aataaatggt gttcaaaagc ttacatctac gatattaaga caaatttatg
    56281 taaaaatgtg aaaaac
//
LOCUS       DACETN010000006        49058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DACETN010000006 DACETN010000000
VERSION     DACETN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.007"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            4..816
                     /locus_tag="IXF98_000360"
     CDS             4..816
                     /locus_tag="IXF98_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1554699.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IXF98_000361"
     CDS             813..1613
                     /locus_tag="IXF98_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1554700.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IXF98_000362"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IXF98_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1554701.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IXF98_000363"
     CDS             3110..4819
                     /locus_tag="IXF98_000363"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1554702.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IXF98_000364"
     CDS             5031..9383
                     /locus_tag="IXF98_000364"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1554703.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IXF98_000365"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IXF98_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1554704.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IXF98_000366"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IXF98_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1554705.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IXF98_000367"
     CDS             11221..11514
                     /locus_tag="IXF98_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1554706.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IXF98_000368"
     CDS             11511..11822
                     /locus_tag="IXF98_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1554707.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IXF98_000369"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IXF98_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1554708.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IXF98_000370"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IXF98_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1554709.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IXF98_000371"
     CDS             complement(14623..14844)
                     /locus_tag="IXF98_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1554710.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IXF98_000372"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IXF98_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IXF98_000373"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IXF98_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1554711.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IXF98_000374"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IXF98_000374"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1554712.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IXF98_000375"
     CDS             20242..21027
                     /locus_tag="IXF98_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1554713.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IXF98_000376"
     CDS             21040..21834
                     /locus_tag="IXF98_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1554714.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IXF98_000377"
     CDS             complement(22014..22466)
                     /locus_tag="IXF98_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1554715.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IXF98_000378"
     CDS             complement(22931..23299)
                     /locus_tag="IXF98_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1554716.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IXF98_000379"
     CDS             23449..23931
                     /locus_tag="IXF98_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1554717.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IXF98_000380"
     CDS             23968..24978
                     /locus_tag="IXF98_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1554718.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IXF98_000381"
     CDS             complement(25093..25806)
                     /locus_tag="IXF98_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554719.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IXF98_000382"
     CDS             complement(25996..26586)
                     /locus_tag="IXF98_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1554720.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IXF98_000383"
     CDS             27070..27297
                     /locus_tag="IXF98_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1554721.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IXF98_000384"
     CDS             27313..28479
                     /locus_tag="IXF98_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1554722.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IXF98_000385"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IXF98_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1554723.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IXF98_000386"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IXF98_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1554724.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IXF98_000387"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IXF98_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1554725.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IXF98_000388"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IXF98_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1554726.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33692..34357)
                     /locus_tag="IXF98_000389"
     CDS             complement(33692..34357)
                     /locus_tag="IXF98_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1554727.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34333..34506
                     /locus_tag="IXF98_000390"
     CDS             34333..34506
                     /locus_tag="IXF98_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1554728.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34551..35288)
                     /locus_tag="IXF98_000391"
     CDS             complement(34551..35288)
                     /locus_tag="IXF98_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554729.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35290..36792)
                     /locus_tag="IXF98_000392"
     CDS             complement(35290..36792)
                     /locus_tag="IXF98_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1554730.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37051..39045
                     /gene="uvrB"
                     /locus_tag="IXF98_000393"
     CDS             37051..39045
                     /gene="uvrB"
                     /locus_tag="IXF98_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1554731.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39055..41871
                     /gene="uvrA"
                     /locus_tag="IXF98_000394"
     CDS             39055..41871
                     /gene="uvrA"
                     /locus_tag="IXF98_000394"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1554732.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42134..43399
                     /locus_tag="IXF98_000395"
     CDS             42134..43399
                     /locus_tag="IXF98_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1554733.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43403..44134
                     /locus_tag="IXF98_000396"
     CDS             43403..44134
                     /locus_tag="IXF98_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1554734.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44269..45153
                     /gene="rapZ"
                     /locus_tag="IXF98_000397"
     CDS             44269..45153
                     /gene="rapZ"
                     /locus_tag="IXF98_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1554735.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45150..46148
                     /locus_tag="IXF98_000398"
     CDS             45150..46148
                     /locus_tag="IXF98_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1554736.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46182..47117
                     /gene="whiA"
                     /locus_tag="IXF98_000399"
     CDS             46182..47117
                     /gene="whiA"
                     /locus_tag="IXF98_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1554737.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47660..48310
                     /locus_tag="IXF98_000400"
     CDS             47660..48310
                     /locus_tag="IXF98_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554738.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48497..>49058
                     /locus_tag="IXF98_000401"
     CDS             48497..>49058
                     /locus_tag="IXF98_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1554739.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16408 a         8404 c        10563 g        13683 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct
    33721 ttagaatatg ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt
    33781 tcaatcgata ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc
    33841 ttccgttatt tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg
    33901 tcaggctttt caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta
    33961 aaacgatccc gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag
    34021 ctatccattc gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat
    34081 aacaaagcga cccaattcca aagtttagct gtaactgata cattttatta aactttgtct
    34141 ggattttatc aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga
    34201 tttgttgtct atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca
    34261 actccaataa aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca
    34321 caactacata atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat
    34381 gtacttgata tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga
    34441 ttgcttgttt gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga
    34501 agataaaaat acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt
    34561 aatactttat tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga
    34621 ggcgttccat cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct
    34681 ttagcgaacc ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt
    34741 ttcatgacgt tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca
    34801 aaaagcatga tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt
    34861 ccaccagaaa gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt
    34921 aatttgattg cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct
    34981 aaaatgatgt tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt
    35041 ccgatatgtt gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca
    35101 ataataattt caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt
    35161 tttcctgaac ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg
    35221 atgtcgttta atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt
    35281 tctgccatct tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat
    35341 caaaatcaag tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc
    35401 aacaatgatc ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt
    35461 gtcttttaat gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg
    35521 cggcaaaatg attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc
    35581 catttgtccg acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact
    35641 tgcgttaaga gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat
    35701 accaggcaac ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat
    35761 atcgacataa atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc
    35821 aaaaatcaca ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct
    35881 ccaaagtcct ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc
    35941 atcagcagtg gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat
    36001 cttatcgtat tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag
    36061 ctcaggattt tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat
    36121 cggtgtttcc aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat
    36181 cattgcatcg attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata
    36241 tttgatagaa tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc
    36301 aacttttgct ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc
    36361 ttttttgaca gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt
    36421 ctttcgttca tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa
    36481 agcttgcaaa gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt
    36541 ggcgatgttt tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa
    36601 ttcaaatgga gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat
    36661 tttttgcatt gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac
    36721 atgaccaaac gcaagtgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg
    36781 ctttcttttc ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg
    36841 ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt
    36901 atgttataaa acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg
    36961 gtataatttt ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg
    37021 aaaaggaatg aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt
    37081 gagcttgtct cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg
    37141 gatggcgtag tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa
    37201 acatatacga tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat
    37261 aacaaaacat tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct
    37321 gttgaatatt ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc
    37381 gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg
    37441 gctactagtt cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt
    37501 tttggattag gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca
    37561 gaactagatc gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat
    37621 atagattttc agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct
    37681 tcaagagatg aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga
    37741 gaagtcaatg cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg
    37801 gcgactcact ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg
    37861 gaattagaac agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt
    37921 ttagaacagc gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga
    37981 attgaaaatt attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta
    38041 ttggatttct ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct
    38101 caaatcagag gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt
    38161 ttccgtttgc catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat
    38221 gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat
    38281 accgttatcc aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt
    38341 ccaatcatgg gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac
    38401 cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc
    38461 aaagaattag ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca
    38521 gaaatcattc gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt
    38581 cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa
    38641 ggctttttac gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa
    38701 gaaggtaaag taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat
    38761 gaaacgtcaa gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca
    38821 aaaacgatca ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat
    38881 acaaaagaag cggtacaagt ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta
    38941 ttgatgaaat tagaaaaaga aatgaaagat gcggcaaaag ctttggattt tgaaacagcc
    39001 gcgaatgtgc gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct
    39061 aacgataaaa ttacgattca tggtgcacgt gcccataact tgaaaaatat agatgtaacg
    39121 attccacgag ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta
    39181 gcatttgata cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat
    39241 gctcgtcagt ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt
    39301 ccggcgattt ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact
    39361 gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt
    39421 cccaacgatc atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg
    39481 gctttgcctg agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga
    39541 caacataaaa aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat
    39601 ggtgagatct atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc
    39661 gcgatcgtca tcgaccggat cattgtgaaa gaagggattc gttctcgcct atttgattct
    39721 ttagaggcgg ccctgcgatt agcagacggc tatgctctgg ttgacgtgat cggtgaagaa
    39781 gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta
    39841 gaaccaagac tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta
    39901 ggtgtcaaat tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt
    39961 caaggtgcta tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg
    40021 caggcttgtg aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag
    40081 catcaggaga tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat
    40141 tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt
    40201 cgttatcatg atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa
    40261 ttaacttgtc agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc
    40321 aatggaacga atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc
    40381 tcaaacttaa cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta
    40441 aatgacagac tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct
    40501 tccggaacat tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg
    40561 aatctttctg gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac
    40621 aacgatcgac tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta
    40681 gtcgaacatg atgaagatac gatgtgggca gcagattatt tgattgacgt cggacctggg
    40741 gccggagaac aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat
    40801 gaaaattctt tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa
    40861 cgccgtaaag gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa
    40921 aatattagtg ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct
    40981 ggaaagagta cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag
    41041 aattctgcta agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc
    41101 atcgatatcg atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca
    41161 agtgtatttg atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt
    41221 tataagaaag ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg
    41281 gatggaatta ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta
    41341 tgtcatggta aacgatataa ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt
    41401 gctgatattt tggaaatgac agtagaggat gctgtagaat tcttcaagca cattccaaag
    41461 attcatcgca aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa
    41521 ccagcaacga cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac
    41581 aaaatctcta atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc
    41641 gatgacatcg cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt
    41701 ttagtgattg aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga
    41761 ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt
    41821 aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag
    41881 atagctgaga tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca
    41941 gtgttctaga atcattacat ggtagttctt tctacactga ttggtataga taaaaacttc
    42001 ctataagttc aatccgttca aaaaagaatt gagcaatcgg aggactttcc tttgattgct
    42061 tttttcttta tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag
    42121 taagtgagag ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg
    42181 aagcgacatg aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg
    42241 ccaagcatat gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga
    42301 agttattcca ggatttgata aagaccctgt atttatcgag acgatgcttc gtttggcaaa
    42361 agaaagatat tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc
    42421 agaattgatc tcccaacata aacaagagct gtcagaatat tttatctgtc catatattga
    42481 ttattcttta tttgaaaggc tgatcaataa agtaagcttt tatgaagttt gtgaagaata
    42541 tgatcttccg tatcctaaaa cattgatcgt cagagaagaa atgttagtaa atggacattt
    42601 ggaacaggaa ttgccttttg aattcccagt tgctttgaaa cctgcaaaca gtgtggagta
    42661 tctttctgtc caatttgaag gaagaaaaaa agcttttatt ttagagacta gagaagagtt
    42721 cgatttaatt ttaggccgga tatacgaagc cggttataaa agtgaaatga ttgtacaaga
    42781 ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca
    42841 tcaagttcgg atgatgtgcc ttggtcatcc actattagaa gatcctacac cagcatcgat
    42901 tggtaattac gttgtgatca tgccagatta taacgaaaaa atctatcaaa ccattaaggc
    42961 atttttggaa aaaatcgaat atacaggctt tgccaatttc gatatgaagt atgatcctag
    43021 agatggagaa tataaattat ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt
    43081 gacattgaac ggcttgaatc tggctcgttt tgtcacagaa gatcgagtgt tcaacaaacc
    43141 ctttgttgaa acgacctatg gaacgaatca atcagataaa gcaagattat ggatgggtgt
    43201 gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca
    43261 aatgatcaaa gaaaatcgtt acggtactac tgtattttat gaaaaagacc gatcaatcaa
    43321 gcgctggttg ttgatgaaat atatgttcca taactatatt ccaagattca aaaagtattt
    43381 tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt attttaggcg gaatgggcac
    43441 gatggcgaca gaaagttttg ttcgcttgat caaccaccga acgaaagcta caaaagatca
    43501 agaatatttg aattatgtct tattcaatca tgcaacagtt cctgatcgta cagcttatat
    43561 tttagatcga tcagaagaaa atccaatgcc gtttctgctg gatgatattg agaaacaaaa
    43621 tttattgcga ccaaacttca ttgtgttgac ttgtaacacc gcacactact tttttgaaga
    43681 gttacaagcg gcaacggata ttcctatctt gcatatgcca agagaagcag caaatgaact
    43741 tgtccgtcag catacgacag ggagagtagc gattctagga acagaaggaa gtatgaaagc
    43801 tggaatctat gaacgcgaag tcaaaaatct tggtttcgaa acagtgattc ctgatactgc
    43861 gcttcaagaa aaaatcaatt acctgatcta tcatgagatc aaggaatcgg attatttgaa
    43921 tcaagagctc tattacgaaa tcttagagga agcggtagag cgcctaaact gtgaaaaagt
    43981 catcctaggt tgcacagaat tatctttgat gcatgaattt gctgaagata atcattaccc
    44041 agtgatcgat gctcaatcta ttttagcgga tcgaacgatc gaacgtgcat tagctgaaag
    44101 aagcgaggca ttggatacag catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc
    44161 taaaagatat ttggtccaaa tctttttgta aaagaaaaag aaaactggta atgatacgcc
    44221 cgggatgata taattttatt atgcacaatg tgagaggggt tcgtaaatat gacagataat
    44281 ttgcaattag tcatcataac aggtatgagt ggtgcaggga aaacagtagc tatccaaagt
    44341 tttgaagata tgggctattt ttgtatcgat aatatgccgc ctcgattgat tccaaaattc
    44401 tgggaattga tcaaagaatc aggtaaagtc acaaaaatgg ctttaggagt ggatttgcgt
    44461 tctcgttcct tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc
    44521 atcgacactt ctatcttatt tttagatgct tcagacgaag aactagtggc tcgctacaaa
    44581 gaaacaagac gagcacatcc aatggcgatg gacggtttga tcactgaagg gatacgtaaa
    44641 gaacgggcga tactagacga cttgaaagcc caagcttccg tgatcattga tacgacaaca
    44701 ttgacaccac gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa
    44761 ggatttcgga tcgaatttgt atcatttggt tttaaatacg ggttgccgat cgattcggat
    44821 attgtgatgg atgtgcgttt tttgcccaac cctcattata tcccggaact aagaccattg
    44881 accggaaaag atcagccagt ctatgattat gtgatgtcct ttgatgaaac gcaatcattc
    44941 taccataaat ttttggatct tctggaaact gtattgcctg gatacattaa agaaggaaaa
    45001 agcagcttga cagttgcaat cggctgtaca ggcggtcaac accgttctgt ggcattgact
    45061 gaacgtttgg caaatgcttt agggaaaaac tataaagtca atgtgacaca tcgggacaag
    45121 gataaaagga aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac
    45181 caaagatcgt agtggtcggc ggtggcactg gtctgccagt tatcctgaaa agtttacgaa
    45241 atcaaggagc agatatcaca gcagtagtga ctgttgcaga tgatggtgga agcagcggtg
    45301 caatcagaga atcgatcgct atggctcctc ctggtgactt gagaaatgtc ttagtggcgt
    45361 tatcggatat gccacaattc tatgaagata ttttccaata ccgtttcaaa aaggaagatc
    45421 agtttttagc caatcatacg atcggtaatc tgatcattgc cgcagtttcc gagatgcggg
    45481 gaagcactta tgaggcgatt caattattag caaaaatgat gcatgtagat ggacatgttt
    45541 atccttcttc agaaactcct ttgaccttgc atgctgtttt taaagacgga acgactgcag
    45601 taggagaatc taaaatcgcg atcaaccgta aaaccattga ccgtgtgtat gtctgcaaca
    45661 caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg
    45721 acatggtcgt tttgggaccc ggaagtttat tcaccagtat ccttccaaat ttagtgatac
    45781 cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca
    45841 tgacccaaaa aggggaaacg gaacatttca cagatgccga tcatatacgt gttttacaca
    45901 agcatttgaa tcagcctttt atcgatacag tattggtcaa tacggaaaaa gtgcctgaag
    45961 attatatgga cccagagatc tatgatgagt atctagtaca agtcgcacac gacttcaacg
    46021 gattgagaaa tgaaggatgt cgagtgattt ccaccgactt tttagaattg aaagatggtg
    46081 gtgtcttcca cgatggagac aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa
    46141 aatattaagc aatttattgt tggagaaagg aggagaatag gatgtcattt gctgcagatg
    46201 tgaagaaaga actaactggt ttggaagttc atcgagaaca tgcgaaagcg gaattagctg
    46261 ctttgctgcg aatgaacggc tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc
    46321 aaacagaaaa tgctgcaatt gccagacgta tctattctct tttgaaagat cattatcagg
    46381 tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg
    46441 tccggttgaa gcaaggcaca aaagatgtgt tgatggatct tgatatcatg gatggtatga
    46501 tgttcaatgg acacgtttcg gacgagatta tgggaaattc gcaaaaaatg cgttcttact
    46561 tacgtggtgc ttttatggcc tcaggttcag tcaataatcc ggaaacgagt cgttatcatt
    46621 tggaaatatt ttctatttat gaacagcata accaagacat ttgcgatatg ttaaattatt
    46681 acggtctaca tgctcgaacg cttgaacggc gcaatggcta tatttcgtat ttaaaaggag
    46741 cagaaaaaat tgctgatttc ttaacattga ttggtgcaac caactcaatg ctccgatttg
    46801 aagatgttcg aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc aattgtgaga
    46861 cagcaaatct gaacaagacg attgatgcgg cttctaaaca aatcgaaaac atcgaattga
    46921 tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc gcagaacttc
    46981 ggcttcaaca tccagaggtc agcttgaaag aattagggga aatgattccc tcaggtgcaa
    47041 tatcaaaatc gggaattaat caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc
    47101 gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta
    47161 tcagtttttt ttaaaagcta tacttgcttt tcttccgtca aactataatt ccaactttac
    47221 tggagtaaca taatgttgaa aggcttctat tgttttttaa tacaatcgta taaaaagcgg
    47281 ggagacaact gaatgaagat tttagtttta tacataaact tttgcttaat atagaagtcc
    47341 tgatatagat aggatcaagt atttatctat aactggagat taattaaaaa ctggaaggaa
    47401 ctgctcgatc aaccttgaga actgtttttc ttacttctgg tcaagattct cgtggttggt
    47461 ggataaggat ggttgataag ttcattggat tagctagtcg aaaacaacca ggtgtaatta
    47521 atgtagaagt tttaacaagg ggatggagtg atttattcag tgataatcat aagttcataa
    47581 atagtaattg ggatgtatgg ttggtatacg ttatacctga acttgaacta tcaagatttg
    47641 gtttagaatt tgagaatcga tgtttataaa gggagggcaa aaaatgtgct tttttacaac
    47701 ttttactatc attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac
    47761 agcattttta ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt
    47821 tggatttttt ctattcacag ttattgtatt tatttggaga actacagcac caccattgcc
    47881 agagattaca tatggagaat ttccgtttag tcttgaatat gaaatagatg aggaaagaat
    47941 tttgattgaa gatacggtga ttgcagaatt tataggaagt tttagaggga atgtgacaac
    48001 tcaacctaga cgaagttgga gtataaaact tttaaataat agtggagagt tgaaagcatt
    48061 tcattatgaa ttcttaataa tagaaagtga agatgtaaca atcactttaa gtagtggctc
    48121 tgctggatat tttatgaatg atggtggaag caagccacag ctcacagtcc gtgatcgtat
    48181 tgcaggtttt atgcattttt ctcactatga cttagaagaa gctcgtaaaa tacttgcaga
    48241 atatgggatc acattaatca attgggaata tgcgccaccg ataagaaatt ttttcaatga
    48301 ttattattag tcttataaaa aacagaatgt aaaatttatg tgcgcgagta tttagttttc
    48361 cattttttgt ttgatagata tatagcattg ttgaagcatg ggatagtggt gccaatttta
    48421 tcaatgaggt tggtcaggca gttaacataa ttggtgatgc tataaatcca tttaattgga
    48481 attggaggaa ttaggtgtgg aaacagtgag attatggata ttcatgaaaa attttcgttc
    48541 ggctttgaga tatgttattc tggaatcaaa atcaggttat tatctgattg acacagatca
    48601 ctatactttt tggggaggct acctttttcc gatttttaat tggataatcc cccataaggt
    48661 tatgaaaatt aacttgcctg cagaagaaat aaatagatta atactcaaca aagatttaca
    48721 aaggaaaaga gagaaagaac attctaatgt tgcttggata ggtccagcat ttgcagtagt
    48781 gacttcttca attgtcggtc ctatgctaga gagtatttat agtgattttt ctattggaat
    48841 acgttattta tttgttactt taagtatagc tgttcttttg tgcataaagg gttatgttag
    48901 caagaagaat aaagatattt taacactaat cggtgtaaac aatttatcta gtactaaaat
    48961 tatactgttt cctgaatcta taaaagaagg atcaaggttg ttgataattt ttataatgct
    49021 tatactgttt tctgttttat ttggggtgat gtttatag
//
LOCUS       DACETN010000007        47697 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DACETN010000007 DACETN010000000
VERSION     DACETN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.008"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            101..544
                     /locus_tag="IXF98_000402"
     CDS             101..544
                     /locus_tag="IXF98_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1554740.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IXF98_000403"
     CDS             complement(550..1734)
                     /locus_tag="IXF98_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1554741.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IXF98_000404"
     CDS             2002..2700
                     /locus_tag="IXF98_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1554742.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IXF98_000405"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IXF98_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1554743.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IXF98_000406"
     CDS             complement(3510..4043)
                     /locus_tag="IXF98_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554744.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IXF98_000407"
     CDS             complement(4340..6721)
                     /locus_tag="IXF98_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1554745.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IXF98_000408"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IXF98_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1554746.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IXF98_000409"
     CDS             7422..7961
                     /locus_tag="IXF98_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1554747.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IXF98_000410"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IXF98_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1554748.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IXF98_000411"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXF98_000411"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IXF98_000412"
     CDS             9068..10234
                     /locus_tag="IXF98_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1554749.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IXF98_000413"
     CDS             10280..11776
                     /locus_tag="IXF98_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1554750.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IXF98_000414"
     CDS             11857..12540
                     /locus_tag="IXF98_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1554751.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IXF98_000415"
     CDS             13185..14375
                     /locus_tag="IXF98_000415"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1554752.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IXF98_000416"
     CDS             14477..15430
                     /locus_tag="IXF98_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1554753.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IXF98_000417"
     CDS             15499..16848
                     /locus_tag="IXF98_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1554754.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IXF98_000418"
     CDS             complement(17031..17354)
                     /locus_tag="IXF98_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554755.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IXF98_000419"
     CDS             17483..18646
                     /locus_tag="IXF98_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554756.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IXF98_000420"
     CDS             18716..20065
                     /locus_tag="IXF98_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1554757.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMVFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IXF98_000421"
     CDS             complement(20110..20541)
                     /locus_tag="IXF98_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554758.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IXF98_000422"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IXF98_000422"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1554759.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IXF98_000423"
     CDS             23771..24367
                     /locus_tag="IXF98_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1554760.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IXF98_000424"
     CDS             24489..25637
                     /locus_tag="IXF98_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1554761.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IXF98_000425"
     CDS             complement(25675..26088)
                     /locus_tag="IXF98_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1554762.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IXF98_000426"
     CDS             26167..26598
                     /locus_tag="IXF98_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1554763.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IXF98_000427"
     CDS             complement(26697..26930)
                     /locus_tag="IXF98_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1554764.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IXF98_000428"
     CDS             27113..28783
                     /locus_tag="IXF98_000428"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1554765.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IXF98_000429"
     CDS             28930..29316
                     /locus_tag="IXF98_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554766.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IXF98_000430"
     CDS             29303..30001
                     /locus_tag="IXF98_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1554767.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IXF98_000431"
     CDS             30006..30470
                     /locus_tag="IXF98_000431"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1554768.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IXF98_000432"
     CDS             30467..31378
                     /locus_tag="IXF98_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1554769.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IXF98_000433"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IXF98_000433"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1554770.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IXF98_000434"
     CDS             32166..33449
                     /locus_tag="IXF98_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1554771.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IXF98_000435"
     CDS             33462..34388
                     /locus_tag="IXF98_000435"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1554772.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IXF98_000436"
     CDS             34407..35687
                     /locus_tag="IXF98_000436"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1554773.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IXF98_000437"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IXF98_000437"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1554774.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IXF98_000438"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IXF98_000438"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1554775.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERTGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IXF98_000439"
     CDS             40012..40788
                     /locus_tag="IXF98_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1554776.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQSSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IXF98_000440"
     CDS             40785..41717
                     /locus_tag="IXF98_000440"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1554777.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IXF98_000441"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IXF98_000441"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1554778.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IXF98_000442"
     CDS             42437..43066
                     /locus_tag="IXF98_000442"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1554779.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IXF98_000443"
     CDS             43054..43368
                     /locus_tag="IXF98_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554780.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IXF98_000444"
     CDS             43406..44047
                     /locus_tag="IXF98_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1554781.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IXF98_000445"
     CDS             44078..44782
                     /locus_tag="IXF98_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1554782.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IXF98_000446"
     CDS             44779..45681
                     /locus_tag="IXF98_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1554783.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45839..47545)
                     /locus_tag="IXF98_000447"
     CDS             complement(45839..47545)
                     /locus_tag="IXF98_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1554784.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15521 a         8349 c        10269 g        13558 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc tgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc gtttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 ggttttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtact ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagtcatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta gcaaacaatt
    45781 tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca ctcattgatt
    45841 acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata atcgtctttt
    45901 ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata tgctttacct
    45961 gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag gcagctaact
    46021 cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg acgtgcgaac
    46081 cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc attgtcagct
    46141 ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa gaaaagtatt
    46201 gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt ttcaggtatc
    46261 cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca atttccagtt
    46321 gagataagac agattcaaga tattggattt catctttggc ttcttggatt tgttcgccaa
    46381 tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt gcattttgat
    46441 ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca taataattag
    46501 gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta agtagttcgc
    46561 catttctacg gtaattctca gcgttttcgg attctttgag ggtttgttca cgttttttca
    46621 atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct ccgccttgtt
    46681 gttttgcccg atctttctct gctttttcgt gataatagct atctaataat tgactgagtg
    46741 ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca atcggtgtga
    46801 aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg atggctgaga
    46861 aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt ttggtcagct
    46921 cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt ggatctagtt
    46981 cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg ttcaagacct
    47041 cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa gtgttttgag
    47101 agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta actaagacga
    47161 tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga tcacccaact
    47221 cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt tcgatgtctt
    47281 ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc ggattatcag
    47341 gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc gcagataata
    47401 ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt tcataaggct
    47461 gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg accatcgcgt
    47521 gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata agtatattca
    47581 ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa agtctagaaa
    47641 aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa attttta
//
LOCUS       DACETN010000008        46894 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DACETN010000008 DACETN010000000
VERSION     DACETN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.009"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="IXF98_000448"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="IXF98_000448"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1554785.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="IXF98_000449"
     CDS             1013..2260
                     /locus_tag="IXF98_000449"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1554786.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="IXF98_000450"
     CDS             2336..2680
                     /locus_tag="IXF98_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554787.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="IXF98_000451"
     CDS             complement(2797..3123)
                     /locus_tag="IXF98_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554788.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="IXF98_000452"
     CDS             complement(3496..4698)
                     /locus_tag="IXF98_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1554789.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="IXF98_000453"
     CDS             4815..5330
                     /locus_tag="IXF98_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1554790.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="IXF98_000454"
     CDS             complement(5529..5975)
                     /locus_tag="IXF98_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1554791.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr"
                     /locus_tag="IXF98_000455"
     CDS             complement(6022..7500)
                     /gene="msr"
                     /locus_tag="IXF98_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1554792.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="IXF98_000456"
     CDS             complement(7854..8483)
                     /locus_tag="IXF98_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1554793.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="IXF98_000457"
     CDS             complement(8467..8931)
                     /locus_tag="IXF98_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1554794.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="IXF98_000458"
     CDS             complement(9418..10686)
                     /locus_tag="IXF98_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1554795.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="IXF98_000459"
     CDS             complement(10917..11654)
                     /locus_tag="IXF98_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1554796.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="IXF98_000460"
     CDS             complement(11721..12290)
                     /locus_tag="IXF98_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1554797.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="IXF98_000461"
     CDS             12418..13290
                     /locus_tag="IXF98_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1554798.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="IXF98_000462"
     CDS             13632..14504
                     /locus_tag="IXF98_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1554799.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="IXF98_000463"
     CDS             14501..16459
                     /locus_tag="IXF98_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554800.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="IXF98_000464"
     CDS             complement(16710..17561)
                     /locus_tag="IXF98_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1554801.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="IXF98_000465"
     CDS             17684..18151
                     /locus_tag="IXF98_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1554802.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="IXF98_000466"
     CDS             complement(18414..18896)
                     /locus_tag="IXF98_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1554803.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="IXF98_000467"
     CDS             complement(18996..19859)
                     /locus_tag="IXF98_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1554804.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20457..21305
                     /locus_tag="IXF98_000468"
     CDS             20457..21305
                     /locus_tag="IXF98_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554805.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21476..21817)
                     /locus_tag="IXF98_000469"
     CDS             complement(21476..21817)
                     /locus_tag="IXF98_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1554806.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21903..22610
                     /locus_tag="IXF98_000470"
     CDS             21903..22610
                     /locus_tag="IXF98_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1554807.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22597..22920
                     /locus_tag="IXF98_000471"
     CDS             22597..22920
                     /locus_tag="IXF98_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1554808.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23009..24046)
                     /locus_tag="IXF98_000472"
     CDS             complement(23009..24046)
                     /locus_tag="IXF98_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1554809.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24493..25476)
                     /locus_tag="IXF98_000473"
     CDS             complement(24493..25476)
                     /locus_tag="IXF98_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1554810.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25892..26662)
                     /locus_tag="IXF98_000474"
     CDS             complement(25892..26662)
                     /locus_tag="IXF98_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554811.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26655..27590)
                     /locus_tag="IXF98_000475"
     CDS             complement(26655..27590)
                     /locus_tag="IXF98_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554812.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28184..29833)
                     /locus_tag="IXF98_000476"
     CDS             complement(28184..29833)
                     /locus_tag="IXF98_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1554813.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30028..30924)
                     /locus_tag="IXF98_000477"
     CDS             complement(30028..30924)
                     /locus_tag="IXF98_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1554814.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30917..31597)
                     /locus_tag="IXF98_000478"
     CDS             complement(30917..31597)
                     /locus_tag="IXF98_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1554815.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31598..32380)
                     /locus_tag="IXF98_000479"
     CDS             complement(31598..32380)
                     /locus_tag="IXF98_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1554816.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32373..33290)
                     /locus_tag="IXF98_000480"
     CDS             complement(32373..33290)
                     /locus_tag="IXF98_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1554817.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33293..33577)
                     /locus_tag="IXF98_000481"
     CDS             complement(33293..33577)
                     /locus_tag="IXF98_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1554818.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34365..37592
                     /gene="fss3"
                     /locus_tag="IXF98_000482"
     CDS             34365..37592
                     /gene="fss3"
                     /locus_tag="IXF98_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1554819.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37705..38019
                     /locus_tag="IXF98_000483"
     CDS             37705..38019
                     /locus_tag="IXF98_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1554820.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38032..38406
                     /locus_tag="IXF98_000484"
     CDS             38032..38406
                     /locus_tag="IXF98_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1554821.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38416..38751
                     /locus_tag="IXF98_000485"
     CDS             38416..38751
                     /locus_tag="IXF98_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1554822.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38834..39979
                     /locus_tag="IXF98_000486"
     CDS             38834..39979
                     /locus_tag="IXF98_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1554823.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            40694..42553
                     /gene="ltrA"
                     /locus_tag="IXF98_000487"
     CDS             40694..42553
                     /gene="ltrA"
                     /locus_tag="IXF98_000487"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1554824.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            42647..42886
                     /locus_tag="IXF98_000488"
     CDS             42647..42886
                     /locus_tag="IXF98_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1554825.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            42964..43638
                     /locus_tag="IXF98_000489"
     CDS             42964..43638
                     /locus_tag="IXF98_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1554826.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            43871..44305
                     /gene="dcm"
                     /locus_tag="IXF98_000490"
     CDS             43871..44305
                     /gene="dcm"
                     /locus_tag="IXF98_000490"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1554827.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            44306..45013
                     /locus_tag="IXF98_000491"
     CDS             44306..45013
                     /locus_tag="IXF98_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1554828.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            45003..45293
                     /locus_tag="IXF98_000492"
     CDS             45003..45293
                     /locus_tag="IXF98_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554829.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            45552..46736
                     /locus_tag="IXF98_000493"
     CDS             45552..46736
                     /locus_tag="IXF98_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1554830.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            46751..46870
                     /locus_tag="IXF98_000494"
     CDS             46751..46870
                     /locus_tag="IXF98_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1554831.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        14567 a         9268 c         8883 g        14176 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatatactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctactg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    20221 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    20281 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    20341 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt
    20401 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    20461 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    20521 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    20581 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    20641 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    20701 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    20761 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcacttta
    20821 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    20881 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    20941 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgcttatt cagcaaaatg
    21001 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    21061 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    21121 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    21181 aagtcgatca tactgacgcc tcttattcaa aacgctactc tataaaaatc ctgaaagaag
    21241 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac
    21301 ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc
    21361 cacaagaaat acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag
    21421 aagacttaac gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata
    21481 agtcatgaca aatttccttc gttgctggat agattttccg gcattgttga tagattttat
    21541 gatacttttc tacattttgt aagagaggtt ccgctgcatc tgtatattgg ataaaattct
    21601 gacaacaact ttcaggtgtg tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc
    21661 ctaatgcagg tccttcctcc acacttaaac aatagaccgt tgcatcgaaa atatcagctt
    21721 ggatttgtag ccatgcaaga tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc
    21781 tgcgacccgc tagttcttta cgtttcaatc ccaccattga caaatgctca ttaaaaattt
    21841 agaatattca caagtttaac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt
    21901 tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct accgttttcg
    21961 gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccgga tttcatccac
    22021 tagtcgtcac gttgatgtcc ctgctggtct atgctggttc tgcccaattt atcacagtca
    22081 gcatgcttgc tagtcacagc ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt
    22141 cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc
    22201 aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat
    22261 tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt
    22321 atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc
    22381 cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc
    22441 aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt
    22501 tgattctggt atatgtcgga ctgattttta tcccaagcaa cttagtgatt gtagttgtaa
    22561 cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg
    22621 ctgactattt taggttgtac tttagcaact tggctttcta gggtcctacc atttgtctta
    22681 ctgaaaaaat tcgatttacc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc
    22741 attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag
    22801 atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa
    22861 agcttattgg tgatcgtctt ggcaggaatt ctttcccttt cgttgattcg cttattataa
    22921 ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca
    22981 aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt
    23041 ttcctaacat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca
    23101 tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag
    23161 caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga
    23221 acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct
    23281 ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgc ttgatttgat
    23341 caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt
    23401 cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt
    23461 tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg
    23521 ctagttgatt caaaatcgaa ccgacgcccc cggatccgtt gatcactaag atttttttcc
    23581 cgcgattggc attttcttct ggagttagac caaatttttc aaataacaat tcataagcgg
    23641 tcaatgaggt caacggcaag gctgcagctt cttcatctga aagatttttt ggtgcaagag
    23701 cgacaatacg ttcgtcaact agttggtatt cttgattgct tcctgctctt gttgttgtac
    23761 ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcgtcacct acagcaacaa
    23821 ctttcccgac tccatcaaaa cctaagatac gtaaagcatt tcttatcccg ttatcttggc
    23881 gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg
    23941 aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca tcgatcggca
    24001 acccttcata aaaaccgact accttcatta attccttttc tgacatacga attcctcctt
    24061 agcttcatca agttggagca tccatacagt gttcatttca atatatatag tatgctattt
    24121 gttccacctt ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt
    24181 tttggttaaa aaagaggttg cgacaagact tttgtcacaa cctcttataa acggtgttct
    24241 cctaccaact cttcggtcac aagtcaaaat attcatcaaa catgagaagc tgttttttga
    24301 aattgctcct tgtgactcag agttaaacgg caggctcaca acctctgata taaacggtgt
    24361 tcaatcaact gaccctcggt attagctcaa aaaacgacaa aatatgaaga gctatttttg
    24421 tttggctgtt cttattactt gatacaggac agctttttca cgacctctaa ttcacggtgt
    24481 tcaagcaaga aatcattgtt cactgaaaag cgttggatgt tctttttcaa aggtctctaa
    24541 gatctctcgt acttcatcag tagatttcgt attcatcaac tgaactcgca agtcatttgc
    24601 tcctggaaat cctttgatat agattttaaa gaaacgatgt agtcctgtga tggaacgcgg
    24661 gatgatttct gaataatgat cttgcagatc tagttgcaga cgaagtagtc cgatcagttc
    24721 tttttctgta tgttccctcg ggtctttttc aaatgcataa ggattcttga aaatccctcg
    24781 gccaatcatg atgccatcaa cgccatattt ctcggccaat tcttctcctg tctggcgatc
    24841 ggggatatct ccattgatcg tgattagtgt ttgtggagcg atttggtcac gtaaagcaat
    24901 gattttagga atgacttccc aatgtgcatc tactttactc atctctttac gcgtacgcaa
    24961 atggacagaa agattggcta tatcttgatg aagtaggtga ctgatccatt cttccatctc
    25021 agacatttcc gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc
    25081 tgcttcaatc aattgtgccg ctacttctgg acgaaggatc aagccactgc cttttccacg
    25141 ttctgctaca ttagggactg ggcagcccat gttgatatcg attccttgga agcccatttc
    25201 tgccatagca atgctcattt ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac
    25261 catcggctgt tcatcttctg taaatgttaa tcttcctcgt acactgtctt ttccatctgg
    25321 atgacagaaa ctgtctgagt ttgtaaactc agtaaagaag acatccggtg ctgctgcatg
    25381 ctttacgaca tggcgaaata caacatctgt tacatcttcc attggtgcta aaacaaaaaa
    25441 aggctttggc agctctttcc aaaaattagt agtcatttat ttgttactct ccattctatc
    25501 aagcagtgaa gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag
    25561 aagcaaagtg aacagcttaa aagatgtttc tgattgagca aaaaaaagac tgtgacaaaa
    25621 atcttatcac agtccatttt ccggaataaa tggtgttcaa aagcttgcat ttgcggtaat
    25681 aagtccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
    25741 tgcagaacag ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt
    25801 attcgctcaa aaaacaacaa aatatgagga actattttcc cgtttgtttc tctaatactc
    25861 aggtcaaaac gctgatttca caacctcttt tctaacttct caatcctctt cctttattga
    25921 tcaaatacca agtaacggcg tacaaaacaa cgatgaataa cacaagtacg atcatcgatg
    25981 ttgctactgg cacatcgatc gttccaagga atccgtaacg gaaaccggag atcatataaa
    26041 cgatcggatt gacttttgaa acagcttgcc aaaagggcgg taacatagag atggcataga
    26101 aaacgccacc tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcat
    26161 cataagactg agcaaaaatc ccgttgagca gtcctgctaa tgaaaacaag attgccgtca
    26221 tcaataatgt aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg
    26281 aaatgatcgt tacgagagag ccgacgagaa tactgcgacc cacaccacca atcacaaagc
    26341 cccaaataat cacatgcgta ggaacaggtg ctaccaagat ttcttcgata tttttctgaa
    26401 atttttgaga gaagaaggac gaagaaacat tggcatacga actagtgata gcagacatca
    26461 taatcaaacc aggaacgatg aattccatat atgaaaaacc acccatgtca ccgatccgcc
    26521 caccgatcat ttttccgaaa ataacaaaat acaaggatgt cgtgatcact ggcggaacca
    26581 gtgtttggac ccatatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg
    26641 cagtaaaata aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc
    26701 aagaacagtt cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt
    26761 cttgtaagct gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt
    26821 gtctgctcat cttcaaatgt acaagcatat ccttcaatca caggtttggt atcatatggc
    26881 gctaaatcaa agataaatgt ttcaaattgc agttttgaca gaagctcttt catacttgta
    26941 ttctcgatca gttcacccga ttggatgatt ccgatgttgc gacaaagcat ttcagcttct
    27001 tctagataat gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcgtagaaac
    27061 tcccacatct cgcgtctcaa ctcgatgtca actcctgctg ttggttcatc caagatcagc
    27121 aaacgtggtt catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa
    27181 agcatacgtg cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg
    27241 ctgcgtttca tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt
    27301 tgctggaccg tttcaaaagg gttaaaattg aactcttgtg gaaccaatcc aatctgttgc
    27361 tttgctcgaa ctagatcctt gtccagatca taaccgaaga cattcacttt tccagatgtt
    27421 ttattgacta aagaagtaat aatcccgatc gttgtagact tcccagctcc atttggccct
    27481 aatagcgcat agaaatctcc ttctttcacc gaaagatcga ttccccgcaa tgcttcgacc
    27541 ccacttccat agatcttttt caaattattt atttcaagcg catttgccat ttatttccta
    27601 tctccttata gacatgattc ttaattgtat catggaatac agcagatgaa aaaacaactt
    27661 ggcttccaca caaaaacaca cctgctaaca aagaggagat gtgttttcta gttcatgatc
    27721 atcttcgata gaataactat ctaaacaact ctggaacttt taaaatcata aaagtaccga
    27781 cagcaactac tacgatgaca tatagaagat ccttataact gaggttcttg tcttttttat
    27841 tcatgttaag ctttcctccg tccttttatc gaaagctttt ttcttttatt ttaaccgaaa
    27901 taaataaaat ggaaatagtt attttcaatt tctgttttcg ctgtttgttt tacggagaac
    27961 tttgccatgg aacgtgcgtc tcgtcgctga tgctaagttt tttcgataaa agctgctaga
    28021 aggaagacag cgtggtttat tttgtatcgt cttttgctaa atcttccaag atcatctgga
    28081 cagcagactc gttcccattg aattctgcca cttttttggc atcaatgtac ttcttttgat
    28141 aatcgtcttt ttggacccac gcatcatgct tcgtttgata aagttaatca aacggatacc
    28201 actgacgctg ttcatcgaac aacaaacttt ctttttgggc ctgttcaact atggatttag
    28261 gataattcaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta
    28321 atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct
    28381 cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag
    28441 atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat
    28501 ttgttccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt
    28561 ccagcaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat
    28621 catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa
    28681 tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag
    28741 attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg actggattaa
    28801 ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa
    28861 actctggtcg gcaagtgatc ggcatatagg tgcgataatt caaaacggct gctgcaactt
    28921 ccaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag
    28981 tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa
    29041 tcaattctgc ttcactcccg ctcttcattc gaaatgaaaa acctaattta gggatgtctc
    29101 ttaatggttt agtttgctgt ttcagctctt tttgaagtgg tgtcgtcatt tttgctaact
    29161 gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg
    29221 tttgtttctt cactagataa tagaccgtat tgaatactaa gccaccgatt gctagataga
    29281 ttcctagtac ttgccctaaa agaagcaaac ctacacccac cagcggaagc aagccaagaa
    29341 aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg
    29401 caaaattatg atcttttttt cctaaacgag cgaattgata ttggattctt tctctcagtt
    29461 gaggattttc ctgataaaaa gcaattagtt tttctaactg ataatcttct ccaaaatcaa
    29521 agctacgtaa acgctgatac aacgcttctg aacctacact agaataggta ctgttgatcc
    29581 cttcaaatac ttcgaacata tccaagtcat accaagtaag atcgtcgatt tcactatccc
    29641 atgaacggaa ttttttctcc gtgagccacg catccttcaa actttcttct ttatcaaatc
    29701 tcggttgata aggaattttt ccccaattac tacgaactgt ttcctttact ttcaaacgtg
    29761 aaaaaatgtc taaaacaact aataaaataa caactgcaat aataattaaa acgataaatt
    29821 ctctttggcc catatccctc tccgttcctt gatttgttag ttttagtata gcactgttgc
    29881 gctatttgaa aagggggtac aaaaataact gtcttcgtta cctaattcgt atcaatcaca
    29941 ccaaaataaa aacaccttcc aaaaaagaag atgtctttaa aacgagatac aattttttgt
    30001 cattcacttc aggtaaatta gagccaatta aaccctccgc aacttgacgg agatggagaa
    30061 gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga
    30121 caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa
    30181 acgctggaag actttttcaa cagaggtttg cgctcctggt ttgataggat tggccactgt
    30241 taatcgaatg aatgaatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg
    30301 caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac
    30361 agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc
    30421 ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat
    30481 ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa
    30541 tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac
    30601 ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga
    30661 aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg
    30721 ttcattttca tccacccatg tattcagtgc atctactagg tccattagat tcttttctcg
    30781 aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt
    30841 agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac
    30901 gagaagcaac agccacttaa tcaaatctaa accccactcc ccacaccgta tgaatataca
    30961 cagcatgtgc ctttgctagt ttccctcgaa gattggaaat atgcatattc actgttttct
    31021 cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt
    31081 ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag
    31141 ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga
    31201 tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc
    31261 gtaacaacag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca
    31321 acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg
    31381 atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta
    31441 gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag
    31501 catcaagaag tttgtatctc tgttcaagaa cctcctccag taaagaatga acagcttcat
    31561 cgtcttcgat aatcaaaata gtttccagca ggaacaccta cttaatgtca gatttttgga
    31621 aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt
    31681 tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg
    31741 taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaacgaagg
    31801 ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga
    31861 atgtcaaagc cactggaaca gataatgtcg ctaatatcag cagaagcaat acaggaaata
    31921 cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa
    31981 gtgtgtaagc aatcatggct agccccgtaa tcaaaaccac gccaagtgtt gtgatcaaaa
    32041 gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat
    32101 tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag
    32161 ccagaagaat ccatgcagcc attttttcca ccattaaaat ccctgttgca ccgttgatga
    32221 ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc
    32281 aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct
    32341 tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat
    32401 tgtaagaaat aatcttccag tttttgccca actaggtgga taccatccac aggaactttt
    32461 gcctctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata
    32521 acacggctag attggacaaa ataatcccta taaccaagag agtcgagaac cgttactgct
    32581 tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct
    32641 ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac cgtagtgagt ggcgatctgt
    32701 gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa
    32761 cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca
    32821 tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc
    32881 atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc
    32941 agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta
    33001 tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt
    33061 cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc
    33121 aaacccataa tagttctcat aaaggtagac ttacccgctc cattctcccc aatcaaacca
    33181 tagatcattc cacgtttgat ttcgatcgat actttatcta atgctttggc agcaccgtat
    33241 tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt
    33301 ctttcttatt gataagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat
    33361 aaagaaagtg taaattttcc aattgaattt caagaaaaat cagactgtct cgcagacgtg
    33421 gttgagttgt ctgccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca
    33481 ccatggtaat cgttgttatc gttgacatca gacgtccttg tatatgcgtt ggaacttctc
    33541 gctgaagcca gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg
    33601 aaaaataata catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa
    33661 agacttttca caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac
    33721 aagtcacaat atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga
    33781 agctgaccac ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt
    33841 atctttttcc cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta
    33901 acatagctaa aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag
    33961 gattaaggta actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact
    34021 cgaacgataa tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac
    34081 ctggtttttt cttcgtatca ttgaataagt ttgaaaaacc ttgatttata aaggttttcc
    34141 cgtaaatata ttttattata tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc
    34201 taatttaaag gcagtatatt tcgtatttta gattaaattt ttaggctcat tcattctcaa
    34261 atccattcta ttcagtcaat aaaaatttta aaaagacttt ataaacaaag atgttcaagc
    34321 gagcatctta tttttttgtg cttaatttga aaggagaaca tttcatgtat aaaaaacaaa
    34381 aaaagaaatt atgtgtatta actattttga gtttggctgt tcaagcaatt gttggagcaa
    34441 ttgctcctac gattactttt gctgatgaaa ttacccatcc acaaacggta accgtagaac
    34501 tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt ccaatgtcag
    34561 aagtcactgt tccgcattat gcagtgtaca acggctttaa acaagatatt ttctgtattg
    34621 aaccaggagt cccgatttac aatgagttca ctcctggcta tgagaaaaac ccgttaccag
    34681 atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt acagatgcag
    34741 atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat acccttcact
    34801 caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca aaaataaatc
    34861 aagccattga cgattatcag aagaaaccaa ccttccataa tagtacggct aaaacagtat
    34921 tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag ttcgatgagg
    34981 ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg taatcagttg ataatcaccc
    35041 caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa gaaatctgct ggtaccggaa
    35101 cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg attgataaac
    35161 ctaataccta cacaatcaaa attgatgtgg aaacggaggg ttctttaaag atcaaaaaag
    35221 tcgataaaga atcgggtgat gttgtaccag gaacggtttt ccatttagac tttggaaaaa
    35281 ctttacctgc aaaagacgtg actactgaca aagaaggcat tgctacattg gatgagattc
    35341 ctcatggtac aaaagtaacc attaccgaaa aatcagtacc ggcgccttat acgattgata
    35401 ccacccccat gactaccacc attaaagcag gcgaaaccat ttacgtgact tcaaaaaacg
    35461 cacgggaaaa agggcaaatc attctggata aaagcggagt tgaaacaggg agtgatcttt
    35521 ggaatgacaa ttattcctta gctggtaata cgtttgccat tcgcaaagac agtccaaccg
    35581 gtgaaatcgt tcaagaaatg accacggatg aaaacggtca tgcggaaaca ccaaaagaga
    35641 ttgctaatgc gttggaattg ggaacctatt atgtgaccga aactaaagct agtcatgggt
    35701 tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta tgccaatcaa accgtagccc
    35761 ttgttaccag taacgtaaaa gggcaaaatc aagaagtcac tggcgaaacc actttgacga
    35821 aagaagacaa agatactggg gataaagcac aaggtaaggc agtctttgag ggtgctgaat
    35881 acaccttgtt tactgctaag gatggaaaag ctgttaaatg gagtgaggct tttaaaccag
    35941 agttagcgaa gggaacgaaa ggttctgatg aaacggtcac tttgacttta gacgaaaaga
    36001 accaagttgc cgttaaacat ttagctatca acgagtatta ttggcaagaa acaaaagcgc
    36061 ccgaaggata tagccttgat gaaacaaagt atcctgtatc catcaaaaag gtggatgata
    36121 gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa agaacaagtg attcgctttg
    36181 gctttgattt ctttaaattt gctggatcag ctgcgggcac taccgaaact ggcttcaatg
    36241 atctgacctt taaagtgtcg ccattggaag ggaccaatga aatcacaggt gccgaagatg
    36301 aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag tttgaaaatc
    36361 ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt caaaagatta
    36421 cgccattaga aatccgttct acttttaagg aaaacaaaga agactttgtg aagagtgagt
    36481 atgtctttac cattaccgaa caagaccaaa aacaaccaat taaaacggtg accgttcctt
    36541 acgagaagct gacgaacaaa gcattttctg ttagtttgaa ccgtttaatg ctctatgatt
    36601 tgcctgagga agaagatagt ttaacttctc ttgcgacttg gaaagacgga aacaaagaat
    36661 taacttctct ggattctacc gagctagttg ataaattgag ttacaatcta catgaaatca
    36721 aagaagactg gtatgtggta gcgcaagcca ttgatgtgga tgcgacaaaa gctgcccaag
    36781 aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac aagcgcaacg ttggcgaaca
    36841 aagagaaaac aggaacttgg aaaattcagc ataaattaac cgccgaacaa gttttaaata
    36901 aaactattgt cttattcaac tatgtgtatg aaaataagga agcttttgaa gcaggcgata
    36961 agccagtagc gaaggatgtt agcttgaata accaagccca aactgtcagt tgtacagtgg
    37021 aacaccatgt ttctatccaa acaaaggccc acctagaaaa cggttctcag acctttactc
    37081 atggtgatgt ggtggatatg tttgatgatg tgtcgattac tcatgatgta ctggatggct
    37141 caaaagaagc tttcgaaaca attctgtatg ctttattacc ggatggtaca aacaaagaaa
    37201 tttggaaatc tggcaagatt gattatgaag taaatgataa agaattcacc aaaactgtgc
    37261 ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac caagtttact tttgcggaaa
    37321 tcaattacga taaagatggg aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat
    37381 ctcaaacctt aacaccaaaa gaagttccaa ccatactaag tacgccgaaa caaccggaaa
    37441 caccaactgt tccaagtggc tctcaagaat ctagtcccac agtgaagacc ttccctcaaa
    37501 ccggggagaa aaattccaat gttttactgt tcattggttt tactttgatt tttgcgacgg
    37561 cgggctatta tttctggaat cgccggaact aaggtgatga gatgatacaa gtaaatccta
    37621 agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc gctatttttg tatccaaaac
    37681 taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg tcgtgccaga tatggctgaa
    37741 acctttggaa agttaagtta tgccggtgaa ggagaaatta taacagaagg atatggacgg
    37801 aatactacgg tgattggtcg cagttatcat ttgtattcca gtaagcaacg ggccgatgat
    37861 attgaggtgg tagtagctgc ggaagccggt gaaaaggatt ttgatcaaga ccaaccgcta
    37921 aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg tgggtttact
    37981 gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa gatgagatta
    38041 acagaaggta ttgtcgtaga ttcaggttta acatttggga agttgcgttt ttccgcatta
    38101 cgccgagaag tacggaagca aaatgaggat ggaacaattg gtaatgaggt aaaagaacga
    38161 acctataatt taaaatcctc tgcacaaggg cgaatgattc aagttagtat cccagctaat
    38221 gtacccttac gcgagtttgc ttatgatgca gaggtagagt tggtcaatcc aatcgtggat
    38281 accgttgcca attatgtttt tcgagaagga actaccgtta attggttcat taaggcggat
    38341 gatttagtct tgaagcggca gccgaatcaa ggaaatccta cgaaccaaaa tgaaagaaag
    38401 aaataggtga agattatgga aattaatgtt gaacatattt tagattgtct cgatcaatat
    38461 ggaaaaggcg agctgactga ggaacaactc gttaaagcat taacctctga tgaaaaaatg
    38521 tttctgatta tacatcaagg tttacttgga gaaaatagcg acagtgaaga agactctgat
    38581 gtactagact ggttaggaga agaagagtct ttctttatag ttgtcgaagt aaatgaaaac
    38641 ttatgtcggc aggcagaatc cattttggaa gaaattggtg tggagatgcc ggatgctatt
    38701 gaaggctttt taaatcaatt ggttgaaacg aaacaactcc cctcctcgta gcagtaaatg
    38761 actagcataa ctttttgaaa ttgcctaatg aaatcctatt gggctttttc gattggaggt
    38821 ggaaaccatc aaaatgtaca aaggacatcg catacgcgca ggagatcagc acttggttta
    38881 tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt
    38941 tcaagagttt agacaatttg atatttctaa gctatcactt tctactattg aaatcgtttg
    39001 gtctataaaa gatttagtat gtctgcttgg aagtctcgct ttttcaggag ctatgatatt
    39061 gctttatatt cacttttttc ttgatcattg gcgaagcttg tggcatcgac aaaagctagc
    39121 tcggatgatt ctagagaacc attggtatga agtgaaacaa actcaaagtg aaggcttttt
    39181 caaagatttg aatagtagcc gaaccagaga gactatcagt tacttcccca aaatctatta
    39241 tcgaatgaaa gatggattac tgtctattcg cgttcaaatt tcacttggga aataccaaga
    39301 tcagctttta aaattggaaa agaagttaga aagcggactg tattgtgagt tagtagaaaa
    39361 ggaactcaaa gactcctatg tggagtatac tttactgtat gatatgatcg ccaatcgaat
    39421 tgggatagac gaagtagtgg cagaaaatgg gactctgcga ttgatgaaaa atcaagtatg
    39481 ggcttatgat tctttacccc atatgttaat cgctggtggc acgggtggag ggaagaccta
    39541 tttcctcctc accattatcg aagcactatt gaagtcagat gcagaattgt ttatcctcga
    39601 tcccaaaaat gcagatttag ctgatttagg tacggtgatg cctcatgttt attcacaaaa
    39661 ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt atgatggctc gtagcaaggc
    39721 aatgaaagaa atgtctaact acaaaacagg agagaattat gcctatcttg gacttccacc
    39781 caacttttta atctttgatg aatacgtggc ttatatggag atgttaacga cgaaagaaag
    39841 tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg ggtcgtcaat ctggtttctt
    39901 tctgattttg gcttgccaaa ggccggatgc gaaatattta ggagacggaa tccgcgatca
    39961 atgtgcgaca tgtttctaac tgaaaaggtt aggcacttaa aagaagaaaa cataagtttc
    40021 cttttaagga gaactgatat tttgagcagt ggaatgatgg ggtaacgccc tgaaacgctc
    40081 ccttaatact ccgactggct gtttaacata tagctgttag atggtcagaa gctcggtgaa
    40141 gtcggctgaa agttaccgta aatatgataa attcatatac gaaagttgtt cagaggtgaa
    40201 tgatgtaacc tataggtcgg aggtctataa aatactcacg gtgagaatgt aagtaatact
    40261 actgacgaac ttgcgaatga aagaatctat acacggactg tatagaaatg tacaggattc
    40321 taccgtatca acggaacaga atgtatgttg tggtttagta agattattgg ttatgaacaa
    40381 ccatgcaatg ttacaggcat tggaaatata caggttcaaa gcaagcacct aaaagtatat
    40441 ataaagataa aaatatcgaa acatgggaag ctggcaatgt ggaggaaact atattccatt
    40501 gaaacatttg tgacgaagaa taataagcac atttatgcca gtgaaagtgg tggcacagta
    40561 ccaatgagga tatagaaata tatcggagga atagccacta gtcaaaagaa atcgttaatt
    40621 aaatgatgta tttcatagaa tcgagtaaga caaaataaac actacaacct aagtaaacaa
    40681 aggtagtggt gctatgaata aagtaaaaac aaataaaaca cttcgtcata gtgaatacta
    40741 tggcatgaca caaatatttg atgaactgta tgtaaaaagt gataaaaaag aagtttttac
    40801 agagctcatg acaattatta tgagtgatga aaatattcga ctagcataca gaaatatcaa
    40861 gaataacaag ggcagttata cagcgagtac cgataagaaa agtattcgag acattaaaac
    40921 actgtcagaa gatacctttc taactgaggt taaagttctt tttcgctgtt acaaaccacg
    40981 agcagttaga cgtgtagaaa ttcccaaacc aaatggaaaa acacgccctc tgggcattcc
    41041 atcaatctgg gataggttaa tacaacagtg tattttacaa gtcttagaac cgatatgcga
    41101 agctaaattt cacaattata gttatggatt tcgaccaaac cgttccacac atcatgcgat
    41161 tgctacctgc aatcgactgg caaatagggg gaagctacac tttgtagtag atgttgatat
    41221 taaaggattc tttgataatg tcaatcactc taaacttttg cgtcaaatgt ggacgattgg
    41281 aatccgtgat aaatggctat taggtattat tcgatccatg ctaaatgctc ctatagtcct
    41341 accaaccggt aaagtcgaac accctaaaaa aggtgtacct caaggtggtg tcctatcccc
    41401 tttactatct aatatcatat tgaatgaatt agattggtgg gttgcttcac aatggcaaaa
    41461 taccacgatt gagacagaaa agattatgga taggcgagat aaaggcaaag gcattgataa
    41521 gactaatcag tatcgggcat taagacgaac aaacttaaaa gaaatataca ttgttcggta
    41581 tgctgacgac ttcaaaatct tttgtagaaa acgaagtcat gcagataaaa tatttcacgc
    41641 aacgaaaatg tggcttaaag aacggttatc acttgaaatt agcgaagaaa aatccaaaat
    41701 tgtcaatctc aaaaaaggta aatcagaata tctaggattt gaattaaagc taatcaagaa
    41761 aggtaaaaaa tacgtagtcg aaagtcatat gagcaaaaaa tcaatgacaa gagttaaaat
    41821 ccaattaaag aaacaattac gtaaagttgc cagaccgaag aatcattgcg aacaagcaaa
    41881 agaaattgga ttatataatt ctatggtgat tggaatccat caatattatg gaattgcaac
    41941 ctgtgtaaat ctcgattgca gtaaaatagc ctatacacta aaaccgttca tcaatcacaa
    42001 actgccgaca aagaaacatg gaaagatatt aaatacattt atcaaatcga agtatggaaa
    42061 aagtaaagaa atgcgatggt tgaataatgt gccaattgta ccgctcaact atgttcagtt
    42121 tagacttgct gtccccctat cagagggaac ttgcaagtac actgaaagtg gacgaagcaa
    42181 gatacacagt aaattaaaat tagacctagc ccttttactt cacatgatga gaaatagtaa
    42241 ttatgaaaga agtattgaat ttgtggataa cagaatatct aaatattctg ctcaaaaggg
    42301 aaaatgtgct atcacgggta aatttctcga atacgaagaa atccattgcc accatatcgt
    42361 accagtaaaa caacagggta cagacaaata cagcaacctc attattattc acaaaaatgt
    42421 tcacgcatta gtccatgcta tcgaagaaaa aatcatacac aaatacctga atgtattaaa
    42481 tctaaccaat gaacaaatag aaaaactaaa tgaattacgt gtcaaagctg gtaatagtgt
    42541 tttaacagtt taacttgaaa aatatatcat acttaacatt cttttgatgg aacgccgtat
    42601 gaagtgaaag tttcatgtac ggtgtgaagc gggggaaaat ctggagatga catcaaagga
    42661 ttacctatcg ctattcaact ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc
    42721 catgatgttt ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta
    42781 tgtggataca ggtggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta
    42841 tgatttctta gattcaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac
    42901 tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt
    42961 aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt
    43021 tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg
    43081 agtcctgggg ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat
    43141 gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaactct
    43201 agtctctttc aaaaagcatc ttcttggttg gtagagcagg catttagata tgtcttagat
    43261 catggctatt cttttattct ggatgcaact tttgcaatat taagtgctga aaaaaatatc
    43321 tcgcgggcgt tgaaaaaaca ttttcgagtg acgattttct atgtttatca agaccctaaa
    43381 atcgcttgga aatttacaca agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag
    43441 gcatttatta acgctttttt caaatcaaga gagaacattg caaaagtaaa gagtaaacat
    43501 ccagatgtat tgcttcatat cataataaaa gattatacga ataatatttc cgaagttcat
    43561 tatgctacgg ataatctaga attattttta ccactagggt atagtaggaa agatttggag
    43621 gttcaattac atgactgaga aagaacaagt aacgaaaatt gtaaagaaat acaataagag
    43681 cattgcggat ttatcggaaa atgctactgc aaaagagttc aaaacggtga ttaaatatgt
    43741 agccgatcag gccaatgaaa aacaacggaa attagttgga ttaaataaaa aatagtagga
    43801 caaattaata atgtatgagc caagagcaaa atgttcttgg cttttttctt tgcaatgaat
    43861 gaggtaatgt atgagatttt tagatttatt cgccggtatt ggtggctttc gtttaggtat
    43921 ggaacaggcg agccatcatt gtatcggttt ttgtgaaatt gataaatttg ctagacgtag
    43981 ttataaagca attcatgata caagtaagga ggtggaaatg catgacatca caagtgtatc
    44041 agatgagttt attcaatccc tcggaccagt ggacatcctt tgcggcggat ttccgtgcca
    44101 agctttttca attgcgggaa agcggcaaag attttccgat actcgaggta ctttattctt
    44161 tgaaatcgct cggttcacca ctcttctccg acctaagctt ttattccttg agaacgtccg
    44221 aggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa cgctggatgg
    44281 attggggtat gacgtggagt ggtaggtgct taactcaaag gcctacattc cccaaagccg
    44341 ggagcggata ttccttatcg gacattctcg agacgcatgt accgaacaag tatttcctat
    44401 cattggaccg tcttcaacat ctgatcaaaa catcagaaat ttggtaaata tcaatccttc
    44461 caatcgagga atgggtggac aagtctatgg ttcagatggc gtagcgccaa ccttaacagg
    44521 tgatgaagga atcaaaatcg cactgccagc aagggatgga atctctgtag caggactatt
    44581 accgggaaag tttgaacaag gtaatcgagt ttatgaagtt actggaaccg cccccacctt
    44641 gtcaacgaaa caaggtggta caaaaatcat gattcgaaaa aatgaaacag aatatcaaga
    44701 ggtgaagcca gaagacagtg tgaatctggc ttttcctaat tccacaagtc gccgaggtcg
    44761 tctgggaaaa cagagtgttc ataccttact aacaggagac caacaagcga tcgtcacgga
    44821 tcagtatcaa attcggaagc taacacctag agagtgttgg cgacttcagg gctttcccga
    44881 ttgggctttt gatcgagatt ctcaagtcaa ttcagatagt cagttgtata agcaagccgg
    44941 aaactctgta acagttccgg tcatttttga tattgccaga agattaaaag agttggatgt
    45001 acatggttta tgatgagttg attggagaaa tttattgggt gattggaaag attcagtcag
    45061 acccagaatt agaagaacaa ttgcgacggt taaaatttga tattcgcaag aatggtgtca
    45121 aagtcgctgg cgatccatat ttaatgaatg aggagacgga tgctcgaatt gaaataaatc
    45181 aagtaattgc agaatttgag cggattgcgg accaagcaaa agaaccagat attcgtcaat
    45241 acttgttaga aatgaaagca gagttggaag taaatggtat tacagatgag taacctaaac
    45301 caacacaact gactgttagg cgctgaccgc ggaggattcg cgaagcagaa atcctccgtg
    45361 gtgctgtgcc tgttcatgga cggcgttagc cgaccatgat gtctcacccc cgtatctaac
    45421 aggggggtac aaaaacacaa aaaataagta agcaaccatt aagaatgctt gttgtatcaa
    45481 agatagtaag taatttgtat aggtgttaac tcatttgaaa aagttaacac cttgaatttg
    45541 gaggtgagaa cttggcacaa cggaatttag attatcggct attaaaagac cgacgcaacg
    45601 aatacggcgt ttcacaaaat aaattggcta cggcttgtgg acttagtcgg ccgtacttaa
    45661 atcagataga aaatggtggc gtaaccgcat ctataaaaac catgaggaaa atttttgacc
    45721 aactggaaag ctttaatccc gatttgcctt tgacgttact gtttgactat gtaaggattc
    45781 gctttcccac aacggatgca cgaaaaatta ttcaagaaat tctccatttg aaatttgagt
    45841 atatgctcca tgaagattat gccttttact cctatcaaga acagtatgtc atgggtgata
    45901 ttgtggtgat gttgtctcat gaagaagata aaggtgtgct tttagaatta aaggggcgag
    45961 gttgccgaca atttgaaact tttttactcg ctcaaaagcg tagctggtac gactttttcg
    46021 aggattgtct aaaggccggt ggcgtgatga aacgcttgga cttggcaatc aatgatcgag
    46081 tggggctatt ggatatacct gagctaacga aaaaatgtca gaaggaagaa tgtatctcct
    46141 tgtttcgtac ctttaaaagt tatcgttccg gggaactttt aaaggctgat gaaaaggacg
    46201 gcatgggcaa taccttatat atcggcagtc tcaagagtga ggtttatttc tgtttgtatg
    46261 aaaaagacta tgaacaatat atcaagttag ggattccatt agaccaaact gagacgaaaa
    46321 atcgttttga gattcgactg aaaaatgacc gtgcttttca tgcgattcaa gatttgttaa
    46381 aaggtcgtag cattgagagc accacgtttt ccattatcaa tcgttacttg cgatttgcgg
    46441 ataaagtaga aggtaaaagg cgaaccaact ggcccttgaa tgaacaatgg gggcgtttta
    46501 ttggacggaa tcggaaagag attcaattaa cttccgagcc aaaaccgtat accattgaac
    46561 gaaccttgaa ttggctaggc cgacaagtcg cccctacgtg gaaaatggct aaggagttgg
    46621 atcgattgaa tcggacaacc tatatccagg atatggtaca gaatgcacgc ttatcagacc
    46681 gacataaaaa gattttggag caacaaagta tggcaattga aaatttgatt atatgaaaag
    46741 aggaatcgtc atgggaattg taaaagatat ttttttggta tttggtggca gtatgcttgg
    46801 tgttactgtg atgtgcttga tgaatgcaag tgcagcagcg gatcgagcaa tggaaaagaa
    46861 aggaaagtga caagatgaat ttcggacaaa attt
//
LOCUS       DACETN010000009        43921 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DACETN010000009 DACETN010000000
VERSION     DACETN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.010"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(137..1528)
                     /locus_tag="IXF98_000495"
     CDS             complement(137..1528)
                     /locus_tag="IXF98_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1554832.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IXF98_000496"
     CDS             complement(1741..2127)
                     /locus_tag="IXF98_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1554833.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IXF98_000497"
     CDS             complement(2183..2806)
                     /locus_tag="IXF98_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1554834.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IXF98_000498"
     CDS             complement(2870..3538)
                     /locus_tag="IXF98_000498"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1554835.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IXF98_000499"
     CDS             complement(3550..3990)
                     /locus_tag="IXF98_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554836.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IXF98_000500"
     CDS             complement(4049..4720)
                     /locus_tag="IXF98_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1554837.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="IXF98_000501"
     CDS             complement(4905..5303)
                     /locus_tag="IXF98_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1554838.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="IXF98_000502"
     CDS             complement(5300..6073)
                     /locus_tag="IXF98_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1554839.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="IXF98_000503"
     CDS             complement(6070..6753)
                     /locus_tag="IXF98_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1554840.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7780)
                     /locus_tag="IXF98_000504"
     CDS             complement(6920..7780)
                     /locus_tag="IXF98_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1554841.1"
                     /translation="MERPNVKKQALELRSTIGSVLKYTALSILSFISIFPFVWMVLGM
                     TNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVFALLISSMAG
                     YGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINSFLVVILPSV
                     STAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAAAAIITFMSS
                     WNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTAIVFFVLQKQ
                     FVQGMIGSVK"
     gene            7761..8684
                     /locus_tag="IXF98_000505"
     CDS             7761..8684
                     /locus_tag="IXF98_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1554842.1"
                     /translation="MTLGRSIVSFSTFSEESGYVINTDSKDTLRKFVQKFRQEVFPGD
                     FFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSYLT
                     KIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEELSL
                     LKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYEKE
                     INLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEICEK
                     VGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            8681..10417
                     /locus_tag="IXF98_000506"
     CDS             8681..10417
                     /locus_tag="IXF98_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1554843.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(10480..11316)
                     /gene="pheA"
                     /locus_tag="IXF98_000507"
     CDS             complement(10480..11316)
                     /gene="pheA"
                     /locus_tag="IXF98_000507"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1554844.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(11313..11822)
                     /locus_tag="IXF98_000508"
     CDS             complement(11313..11822)
                     /locus_tag="IXF98_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1554845.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(11815..13110)
                     /gene="aroA"
                     /locus_tag="IXF98_000509"
     CDS             complement(11815..13110)
                     /gene="aroA"
                     /locus_tag="IXF98_000509"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1554846.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(13123..14214)
                     /locus_tag="IXF98_000510"
     CDS             complement(13123..14214)
                     /locus_tag="IXF98_000510"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1554847.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(14231..15397)
                     /gene="aroC"
                     /locus_tag="IXF98_000511"
     CDS             complement(14231..15397)
                     /gene="aroC"
                     /locus_tag="IXF98_000511"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1554848.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(15399..16463)
                     /gene="aroB"
                     /locus_tag="IXF98_000512"
     CDS             complement(15399..16463)
                     /gene="aroB"
                     /locus_tag="IXF98_000512"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1554849.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(16496..17518)
                     /gene="aroF"
                     /locus_tag="IXF98_000513"
     CDS             complement(16496..17518)
                     /gene="aroF"
                     /locus_tag="IXF98_000513"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1554850.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(18177..19151)
                     /locus_tag="IXF98_000514"
     CDS             complement(18177..19151)
                     /locus_tag="IXF98_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1554851.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(19132..21015)
                     /locus_tag="IXF98_000515"
     CDS             complement(19132..21015)
                     /locus_tag="IXF98_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1554852.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(21033..23258)
                     /locus_tag="IXF98_000516"
     CDS             complement(21033..23258)
                     /locus_tag="IXF98_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1554853.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            23373..24407
                     /locus_tag="IXF98_000517"
     CDS             23373..24407
                     /locus_tag="IXF98_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1554854.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(24474..25487)
                     /locus_tag="IXF98_000518"
     CDS             complement(24474..25487)
                     /locus_tag="IXF98_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1554855.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(25565..25753)
                     /locus_tag="IXF98_000519"
     CDS             complement(25565..25753)
                     /locus_tag="IXF98_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1554856.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(26193..26321)
                     /locus_tag="IXF98_000520"
     CDS             complement(26193..26321)
                     /locus_tag="IXF98_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1554857.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(26324..27697)
                     /gene="rlmD"
                     /locus_tag="IXF98_000521"
     CDS             complement(26324..27697)
                     /gene="rlmD"
                     /locus_tag="IXF98_000521"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1554858.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(27808..28863)
                     /locus_tag="IXF98_000522"
     CDS             complement(27808..28863)
                     /locus_tag="IXF98_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1554859.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(28873..30303)
                     /gene="gatB"
                     /locus_tag="IXF98_000523"
     CDS             complement(28873..30303)
                     /gene="gatB"
                     /locus_tag="IXF98_000523"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1554860.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(30300..31772)
                     /gene="gatA"
                     /locus_tag="IXF98_000524"
     CDS             complement(30300..31772)
                     /gene="gatA"
                     /locus_tag="IXF98_000524"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1554861.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(31772..32077)
                     /gene="gatC"
                     /locus_tag="IXF98_000525"
     CDS             complement(31772..32077)
                     /gene="gatC"
                     /locus_tag="IXF98_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1554862.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVTETEDGFIKVPAIIDNGEAGA"
     gene            complement(32183..34219)
                     /gene="ligA"
                     /locus_tag="IXF98_000526"
     CDS             complement(32183..34219)
                     /gene="ligA"
                     /locus_tag="IXF98_000526"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1554863.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(34236..36470)
                     /gene="pcrA"
                     /locus_tag="IXF98_000527"
     CDS             complement(34236..36470)
                     /gene="pcrA"
                     /locus_tag="IXF98_000527"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1554864.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDLEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            36625..38184
                     /locus_tag="IXF98_000528"
     CDS             36625..38184
                     /locus_tag="IXF98_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1554865.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(38295..40226)
                     /locus_tag="IXF98_000529"
     CDS             complement(38295..40226)
                     /locus_tag="IXF98_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1554866.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(40244..41158)
                     /gene="pfkB"
                     /locus_tag="IXF98_000530"
     CDS             complement(40244..41158)
                     /gene="pfkB"
                     /locus_tag="IXF98_000530"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1554867.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(41155..41907)
                     /locus_tag="IXF98_000531"
     CDS             complement(41155..41907)
                     /locus_tag="IXF98_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1554868.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(42067..42276)
                     /locus_tag="IXF98_000532"
     CDS             complement(42067..42276)
                     /locus_tag="IXF98_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554869.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(42287..43615)
                     /locus_tag="IXF98_000533"
     CDS             complement(42287..43615)
                     /locus_tag="IXF98_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554870.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        12185 a         9504 c         7862 g        14370 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctacctttt
     7801 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     7861 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     7921 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     7981 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     8041 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     8101 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     8161 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     8221 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     8281 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     8341 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     8401 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     8461 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     8521 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     8581 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     8641 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     8701 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     8761 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     8821 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     8881 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     8941 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     9001 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     9061 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     9121 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     9181 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     9241 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     9301 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     9361 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     9421 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     9481 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     9541 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     9601 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     9661 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     9721 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     9781 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     9841 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     9901 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     9961 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
    10021 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
    10081 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
    10141 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
    10201 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
    10261 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
    10321 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
    10381 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
    10441 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
    10501 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
    10561 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
    10621 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
    10681 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
    10741 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
    10801 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
    10861 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
    10921 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
    10981 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
    11041 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
    11101 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
    11161 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
    11221 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
    11281 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
    11341 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
    11401 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
    11461 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
    11521 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    11581 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    11641 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    11701 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    11761 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    11821 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    11881 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    11941 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    12001 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    12061 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    12121 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    12181 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    12241 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    12301 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    12361 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    12421 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    12481 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    12541 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    12601 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    12661 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    12721 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    12781 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    12841 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    12901 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    12961 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    13021 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    13081 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    13141 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    13201 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    13261 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    13321 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    13381 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    13441 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    13501 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    13561 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    13621 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    13681 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    13741 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    13801 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    13861 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    13921 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    13981 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    14041 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    14101 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    14161 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    14221 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    14281 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    14341 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    14401 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    14461 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    14521 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    14581 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    14641 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    14701 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    14761 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    14821 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    14881 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    14941 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    15001 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    15061 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    15121 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    15181 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    15241 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    15301 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    15361 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    15421 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    15481 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    15541 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    15601 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    15661 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    15721 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    15781 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    15841 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    15901 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    15961 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    16021 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    16081 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    16141 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    16201 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    16261 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    16321 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    16381 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    16441 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    16501 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    16561 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    16621 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    16681 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    16741 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    16801 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    16861 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    16921 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    16981 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    17041 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    17101 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    17161 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    17221 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    17281 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    17341 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    17401 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    17461 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    17521 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    17581 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    17641 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    17701 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    17761 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    17821 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    17881 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    17941 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    18001 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    18061 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    18121 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    18181 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    18241 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    18301 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    18361 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    18421 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    18481 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    18541 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    18601 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    18661 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    18721 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    18781 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    18841 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    18901 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    18961 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    19021 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    19081 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    19141 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    19201 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    19261 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    19321 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    19381 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    19441 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    19501 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    19561 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    19621 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    19681 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    19741 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    19801 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    19861 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    19921 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    19981 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    20041 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    20101 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    20161 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    20221 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    20281 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    20341 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    20401 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    20461 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    20521 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    20581 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    20641 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    20701 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    20761 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    20821 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    20881 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    20941 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    21001 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    21061 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    21121 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    21181 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    21241 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    21301 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    21361 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    21421 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    21481 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    21541 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    21601 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    21661 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    21721 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    21781 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    21841 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    21901 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    21961 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    22021 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    22081 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    22141 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    22201 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    22261 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    22321 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    22381 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    22441 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    22501 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    22561 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    22621 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    22681 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    22741 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    22801 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    22861 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    22921 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    22981 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    23041 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    23101 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    23161 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    23221 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    23281 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    23341 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    23401 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    23461 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    23521 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    23581 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    23641 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    23701 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    23761 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    23821 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    23881 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    23941 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    24001 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    24061 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    24121 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    24181 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    24241 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    24301 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    24361 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    24421 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    24481 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    24541 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    24601 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    24661 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    24721 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    24781 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    24841 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    24901 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    24961 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    25021 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    25081 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    25141 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    25201 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    25261 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    25321 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    25381 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    25441 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    25501 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    25561 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    25621 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    25681 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    25741 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    25801 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    25861 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    25921 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    25981 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    26041 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    26101 ttattggcaa atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa
    26161 agttaacact tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt
    26221 ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt
    26281 ttctttttga tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga
    26341 atgaagcgac taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct
    26401 tcacggcata acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac
    26461 atgagatata gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat
    26521 ctaatccttt tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc
    26581 attttttcat aactttttct gcttttccga cttcataatg aacattttcg attttattat
    26641 ttaaagcatt gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct
    26701 tatccactct gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca
    26761 caatgtcttt tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt
    26821 gttgagtatt tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct
    26881 gatcttcgat atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg
    26941 ttttttcttc attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag
    27001 cttcagcaat ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca
    27061 tttcatggga ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt
    27121 cttcattata tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt
    27181 gatcgatgac aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct
    27241 ttttgtaaaa accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat
    27301 tacggtagcc gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct
    27361 tagcaatctt cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat
    27421 acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct
    27481 gacgatcagg tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg
    27541 cttttactac tagtatttct acttcttctc cagggagtgc attttcgata aataatggat
    27601 aaccatcgat ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat
    27661 atcgttcgtt tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt
    27721 ttcaatccta ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac
    27781 cttactacgt tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat
    27841 ttttccgtca ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt
    27901 gctgacaacc tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc
    27961 ggtattggca aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc
    28021 gccgtattca ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac
    28081 gaacaaactg cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt
    28141 cagcatgagt gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa
    28201 agaaaacttc ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga
    28261 gttcgtcagc cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt
    28321 caagcgcatt ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc
    28381 aagataacca aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata
    28441 gccacctgca gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac
    28501 ggtttggtgt ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa
    28561 tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc
    28621 aagcggagcg attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac
    28681 tagatcaaaa cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg
    28741 tgtagttgca taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc
    28801 caaatttttt ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt
    28861 cattttcagc ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg
    28921 atttgcttgt cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc
    28981 ttttccattt ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat
    29041 tggcagtagt ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg
    29101 ggcttctccg ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat
    29161 cgttccatcg gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc
    29221 agctaattcc agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga
    29281 tgcttgcttg gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt
    29341 tgtaagtgtc aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc
    29401 gcgacgagaa gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc
    29461 gatcacgaat ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt
    29521 gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat
    29581 ttctcctcct gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt
    29641 cacgaaagat aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc
    29701 ataagggcga agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc
    29761 gctgacttct gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc
    29821 tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa
    29881 atcaacgtaa gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg
    29941 gacacgttcg atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc
    30001 atgaccgatt ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa
    30061 gtaatttttc cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc
    30121 tttcatcccg aattccagcg cagctttatt cattactggc agcacgcctg gataacccca
    30181 gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa
    30241 aatttttgag ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt
    30301 cattcgtctt tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca
    30361 aacgcatatg ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat
    30421 ccaactggta atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag
    30481 ttgacaggga tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca
    30541 ccgattccga atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag
    30601 actttttcaa aatctcgttt gatcaatgta cggacttgtc ctgctttttt gaagtaagcg
    30661 tcataataac ccgcgcttaa ggagaatgta cctaacatga tacggcgttt tacttcttca
    30721 ccgaatcctt ctgaacgtga gttgacatat acatcatcta atgatttcac attttcagag
    30781 cgataaccgt agcggatacc atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca
    30841 atgatataat aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca
    30901 gcacctaatg aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc
    30961 acaccttcgc ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc
    31021 agtccctctg caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa
    31081 ccgctgatag ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt
    31141 tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt
    31201 ttcatcccta cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta
    31261 cctaaagaaa ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca
    31321 ccaggtactt ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta
    31381 cttccaccca tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga
    31441 tagattttat ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat
    31501 gcagcggttg tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca
    31561 gcaaggacgt tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct
    31621 ttttcttcgt tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt gattcgttca
    31681 aatgttgttt ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt
    31741 tcttctagag aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga
    31801 ttgcgggtac tttgataaag ccgtcttctg tttccgtcac atttttcaat aattcatcgc
    31861 ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg
    31921 acgtaaatgg aacaccttcc gtatctactt cttccagcat ttcaaccata tcgatgattt
    31981 tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt
    32041 tggcaacgtg tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag
    32101 ttaaaaactt tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa
    32161 tttgtctgaa aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg
    32221 ccatttgatc ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc
    32281 cagcatcttc tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc
    32341 cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag
    32401 taaggactac tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa
    32461 gccctttgta ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt
    32521 cttcgttact gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg
    32581 caataatcga ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta
    32641 aaatttttgc tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga
    32701 ccgaattatc tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta
    32761 ttttgtccaa cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta
    32821 ctagtttttt atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg
    32881 catttcttga aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga
    32941 tgcaccgtaa ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat
    33001 gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa
    33061 cttctgggat gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct
    33121 tcaacttaat ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc
    33181 ggactggtgt catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga
    33241 tgttttcgat tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg
    33301 gtgctttgat agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga
    33361 ttccatcgat ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc
    33421 acacttcatc aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt
    33481 ctagctcttc tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg
    33541 tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag
    33601 aaccagctgc ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt
    33661 ggttcaattg gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca
    33721 ttggttcttt taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt
    33781 ctcctacagt tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca
    33841 aagaaacgga aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg
    33901 ctttttgtac acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac
    33961 tgtaaagcgg aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc
    34021 gttgagtggg agaatcagac gtgatcaaat caggatattg ttcctcgatt gctactaatt
    34081 ctgcgtattc tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt
    34141 cgcgagacca ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa
    34201 tcgtcatttc atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag
    34261 gcagccaaaa gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc
    34321 gacgtcccgc ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct
    34381 ccaggtttcc agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc
    34441 tctggctgtt tatagctata tgctgttttt ttagcgaata caggcgtttt tttaggcgta
    34501 ggacgcatgc cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt
    34561 cggttatatt gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct
    34621 tcttctgctc gcgtgatccc cacatatgcg agacggcgtt cttcctccag ttcgctttct
    34681 tccatcaatg ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact
    34741 ggaaattcta atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct
    34801 tctaaattgt caatgtcgga aacaagtgcc agatcattta agaagaccgt tagtttttct
    34861 tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg
    34921 accgttaaaa attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt
    34981 tttaagtctt ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc
    35041 aagtatggga tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt
    35101 cctgcttttc caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat
    35161 tcatgaagag aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg
    35221 ggcgaattaa cgatacgctc aaaactgatt gaatcttgag gatttaccag cacattcaaa
    35281 tacgctaaaa tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta
    35341 taaggaatat ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg
    35401 tacaagatgg caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccgg
    35461 tctacgataa aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt
    35521 ccttctttgt tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact
    35581 tggtttgctg cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact
    35641 gcagcatctg gataatcttt ttccaaatcc aagatgttct gcatatctgc accgcgccag
    35701 ccgtaaatac tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat
    35761 aaattgacta gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa
    35821 tggaatttgt tttgataata cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta
    35881 ttcatgatca ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca
    35941 gcataacatt ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga
    36001 ggggtctgca ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat
    36061 tttttcggat caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa
    36121 gaatcgatga ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata
    36181 cgcacacaca tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc
    36241 gcattgacac gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg
    36301 ttccaaggat tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc
    36361 gtctttccac ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct
    36421 tctttctgac gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc
    36481 ctttctttta tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt
    36541 tcaaaatcat ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa
    36601 attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag
    36661 actcagatat attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt
    36721 agcctgtttc gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg
    36781 agtcgggaac agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt
    36841 ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta
    36901 gagggacagc ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc
    36961 attggcggtg tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata
    37021 cagcttggcg catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa
    37081 gaaaagattt taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct
    37141 atcgcaggat tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg
    37201 ctgacatcat ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg
    37261 agacctgttt tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta
    37321 gtgatcttag gagtacttct aggcatctta ggttttattt atcagaatgt cttgctggct
    37381 ttgccaaaat tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc
    37441 ttgctgattt tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc
    37501 gttttagctt ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt
    37561 cgattcgttt tttccatgat ttcttacggt gggggtcttc caggggggat tttccttccg
    37621 atattgacat tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg
    37681 atggatccaa tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg
    37741 attggaaaag caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat
    37801 catctgatgc ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc
    37861 ggtaatccaa tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca
    37921 ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga
    37981 ttaatggtac gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga
    38041 aatcaggaaa tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc
    38101 ttgactgatg aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc
    38161 acaaatttga agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat
    38221 ctacactact tgattctata tgattctata aaaaaagaag aggctgggaa aacccagcct
    38281 cttctttttt atttctatgc ttctgctact gcaggagctt ttctcaataa accataaatc
    38341 acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt
    38401 aagaatacaa cgaaaatccc gccgtgagga gccagtaatt tgattccgaa agctccgact
    38461 aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct
    38521 gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa
    38581 ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt
    38641 gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt
    38701 gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg
    38761 cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca
    38821 ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc
    38881 agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta
    38941 attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa
    39001 ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat
    39061 gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat
    39121 cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta
    39181 gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg
    39241 tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt
    39301 gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct
    39361 tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct
    39421 agattgatca actcttctgg tttacggata ccatcactga caggacgatt gatcagttct
    39481 tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca
    39541 cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact
    39601 ttgatctcca cacccatttc tttcgctttt ttcttcaaag catcttctgc catgtaagtg
    39661 tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca
    39721 gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca
    39781 gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg
    39841 aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt
    39901 gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca
    39961 taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt
    40021 ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct
    40081 agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg
    40141 accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct
    40201 ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga
    40261 tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag
    40321 ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc
    40381 tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa tttttcaatg
    40441 ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc
    40501 cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc ccatacggaa
    40561 tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg tgattgtttg
    40621 gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa
    40681 tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc
    40741 gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta
    40801 atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag ccattgatct
    40861 cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga
    40921 acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc
    40981 ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga
    41041 tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta
    41101 acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg
    41161 tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg
    41221 ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa
    41281 tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac
    41341 agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc
    41401 cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac
    41461 agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac
    41521 taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa
    41581 aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat
    41641 ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc
    41701 actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc
    41761 tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt
    41821 caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat
    41881 tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata
    41941 ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc
    42001 aaaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt ctatcatcaa
    42061 gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt
    42121 tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc
    42181 gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc
    42241 cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat attttttgta
    42301 aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac
    42361 ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat
    42421 cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat
    42481 agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat
    42541 attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga
    42601 taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag
    42661 catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata tcataacttt
    42721 ttgttacaaa aaatgaagca ttctttttag gaacgggttg catttgtgca ttctgatact
    42781 ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac
    42841 cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa
    42901 acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga
    42961 cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag
    43021 ggatgatttg atgataatag cgactataat acatttccca acacgaagtg aaacataaac
    43081 cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt
    43141 gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag
    43201 agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc
    43261 catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa
    43321 cagagataga caatacattt tttatcaata attgattatc ttccaagcga aaactacatt
    43381 cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt
    43441 tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg
    43501 ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt
    43561 cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca atcattttct
    43621 cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa aactaaaact
    43681 ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa gaaacaaaga
    43741 ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac ttttcgaatc
    43801 atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc gatttctaaa
    43861 accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta agctcaaaaa
    43921 a
//
LOCUS       DACETN010000010        43704 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DACETN010000010 DACETN010000000
VERSION     DACETN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.011"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(130..654)
                     /locus_tag="IXF98_000534"
     CDS             complement(130..654)
                     /locus_tag="IXF98_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554871.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(647..1120)
                     /gene="tsaE"
                     /locus_tag="IXF98_000535"
     CDS             complement(647..1120)
                     /gene="tsaE"
                     /locus_tag="IXF98_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1554872.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1243..2223)
                     /gene="pta"
                     /locus_tag="IXF98_000536"
     CDS             complement(1243..2223)
                     /gene="pta"
                     /locus_tag="IXF98_000536"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1554873.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2376..3056)
                     /locus_tag="IXF98_000537"
     CDS             complement(2376..3056)
                     /locus_tag="IXF98_000537"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1554874.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3182..4051
                     /locus_tag="IXF98_000538"
     CDS             3182..4051
                     /locus_tag="IXF98_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1554875.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4077..4592
                     /locus_tag="IXF98_000539"
     CDS             4077..4592
                     /locus_tag="IXF98_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554876.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4639..5202)
                     /locus_tag="IXF98_000540"
     CDS             complement(4639..5202)
                     /locus_tag="IXF98_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554877.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5257..5436)
                     /locus_tag="IXF98_000541"
     CDS             complement(5257..5436)
                     /locus_tag="IXF98_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554878.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5513..6022)
                     /locus_tag="IXF98_000542"
     CDS             complement(5513..6022)
                     /locus_tag="IXF98_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554879.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6118..6498)
                     /locus_tag="IXF98_000543"
     CDS             complement(6118..6498)
                     /locus_tag="IXF98_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1554880.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6828..7577
                     /locus_tag="IXF98_000544"
     CDS             6828..7577
                     /locus_tag="IXF98_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1554881.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7761..9038)
                     /locus_tag="IXF98_000545"
     CDS             complement(7761..9038)
                     /locus_tag="IXF98_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554882.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9117..9761)
                     /gene="pcp"
                     /locus_tag="IXF98_000546"
     CDS             complement(9117..9761)
                     /gene="pcp"
                     /locus_tag="IXF98_000546"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1554883.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9960..10904)
                     /locus_tag="IXF98_000547"
     CDS             complement(9960..10904)
                     /locus_tag="IXF98_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1554884.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10906..11592)
                     /locus_tag="IXF98_000548"
     CDS             complement(10906..11592)
                     /locus_tag="IXF98_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1554885.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12066..12386
                     /locus_tag="IXF98_000549"
     CDS             12066..12386
                     /locus_tag="IXF98_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1554886.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12540..13445
                     /locus_tag="IXF98_000550"
     CDS             12540..13445
                     /locus_tag="IXF98_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1554887.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSYKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSIKGEEEK"
     gene            13442..14215
                     /locus_tag="IXF98_000551"
     CDS             13442..14215
                     /locus_tag="IXF98_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1554888.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14396..15820)
                     /locus_tag="IXF98_000552"
     CDS             complement(14396..15820)
                     /locus_tag="IXF98_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1554889.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16031..16477
                     /gene="nrdI"
                     /locus_tag="IXF98_000553"
     CDS             16031..16477
                     /gene="nrdI"
                     /locus_tag="IXF98_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1554890.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16542..18524)
                     /locus_tag="IXF98_000554"
     CDS             complement(16542..18524)
                     /locus_tag="IXF98_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554891.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18538..19434)
                     /gene="murQ"
                     /locus_tag="IXF98_000555"
     CDS             complement(18538..19434)
                     /gene="murQ"
                     /locus_tag="IXF98_000555"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1554892.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19437..20498)
                     /locus_tag="IXF98_000556"
     CDS             complement(19437..20498)
                     /locus_tag="IXF98_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1554893.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20709..21236)
                     /locus_tag="IXF98_000557"
     CDS             complement(20709..21236)
                     /locus_tag="IXF98_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1554894.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21303..22805)
                     /locus_tag="IXF98_000558"
     CDS             complement(21303..22805)
                     /locus_tag="IXF98_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1554895.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23063..24184)
                     /locus_tag="IXF98_000559"
     CDS             complement(23063..24184)
                     /locus_tag="IXF98_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1554896.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24310..25251
                     /locus_tag="IXF98_000560"
     CDS             24310..25251
                     /locus_tag="IXF98_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1554897.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25401..26297)
                     /locus_tag="IXF98_000561"
     CDS             complement(25401..26297)
                     /locus_tag="IXF98_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1554898.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26566..27906
                     /locus_tag="IXF98_000562"
     CDS             26566..27906
                     /locus_tag="IXF98_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1554899.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28124..28567
                     /locus_tag="IXF98_000563"
     CDS             28124..28567
                     /locus_tag="IXF98_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1554900.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29071..30489)
                     /gene="nhaC"
                     /locus_tag="IXF98_000564"
     CDS             complement(29071..30489)
                     /gene="nhaC"
                     /locus_tag="IXF98_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1554901.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30892..32115)
                     /locus_tag="IXF98_000565"
     CDS             complement(30892..32115)
                     /locus_tag="IXF98_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1554902.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32146..32985)
                     /locus_tag="IXF98_000566"
     CDS             complement(32146..32985)
                     /locus_tag="IXF98_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1554903.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(33002..33682)
                     /locus_tag="IXF98_000567"
     CDS             complement(33002..33682)
                     /locus_tag="IXF98_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554904.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33684..34757)
                     /locus_tag="IXF98_000568"
     CDS             complement(33684..34757)
                     /locus_tag="IXF98_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1554905.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35404..36075)
                     /locus_tag="IXF98_000569"
     CDS             complement(35404..36075)
                     /locus_tag="IXF98_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1554906.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36162..38840)
                     /gene="mgtA"
                     /locus_tag="IXF98_000570"
     CDS             complement(36162..38840)
                     /gene="mgtA"
                     /locus_tag="IXF98_000570"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1554907.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39499..39936
                     /gene="ndk"
                     /locus_tag="IXF98_000571"
     CDS             39499..39936
                     /gene="ndk"
                     /locus_tag="IXF98_000571"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1554908.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39958..40824)
                     /locus_tag="IXF98_000572"
     CDS             complement(39958..40824)
                     /locus_tag="IXF98_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1554909.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40984..43077)
                     /locus_tag="IXF98_000573"
     CDS             complement(40984..43077)
                     /locus_tag="IXF98_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1554910.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12620 a         9155 c         7723 g        14206 t
ORIGIN      
        1 gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca cgacttttgt
       61 cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct cactcttttc
      121 ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt cacctagatc
      181 tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc ggtaaaggtg
      241 tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta cacccatttc
      301 cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc cccaatattc
      361 ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac tgtctgcctt
      421 gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata aattattatt
      481 gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca ggagttctgc
      541 ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca aaagaatgtc
      601 ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac tcatgatttt
      661 cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg tatgagatta
      721 gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt tccaaataat
      781 cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc ccatcacctt
      841 caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa atatccatat
      901 gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc ggacttttga
      961 tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt ttacccgcac
     1021 ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca attgcctctg
     1081 cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt ctcccgctct
     1141 tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa tgaactagtc
     1201 aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa tcaatgtttg
     1261 tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac gagacaaatc
     1321 agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt taccgaaacg
     1381 ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa agactgttgc
     1441 ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa cataagaagc
     1501 atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag cgattttagt
     1561 tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg agaagctcat
     1621 cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat ctacggcgat
     1681 ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa agacatattt
     1741 ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga tgccaggctt
     1801 tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga tcgctccaga
     1861 gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat tcacatcttt
     1921 taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa caaaagtatc
     1981 caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc tagatgtttt
     2041 gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta aaatgggttc
     2101 cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat cagtagcttc
     2161 cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat caaacaattc
     2221 cacaagatga cctccagacg tttttatatg tattatcata cactattcca gaaaaaaaga
     2281 acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt tcacatacaa
     2341 agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg cagttgccaa
     2401 tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa gggtttggaa
     2461 ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat gatgatatgg
     2521 cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa gataaacacg
     2581 attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct ttcccaacct
     2641 ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt gagtaaaagg
     2701 actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat tccaagatca
     2761 tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc aggttgcaca
     2821 gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc taaaatcacc
     2881 actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata catatctgga
     2941 tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag ataataatct
     3001 tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc tttcaaaaac
     3061 tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga caatcttcac
     3121 cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa cgataatcat
     3181 tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc atatgagtgt
     3241 ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg agttcatggt
     3301 cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag gaatcgattg
     3361 tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt cattgtttac
     3421 cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg ctaacggtat
     3481 ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga aacgaattga
     3541 aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa tggcgattgc
     3601 catggcctca gctaagctgg agttattgca catcacatac gtggacagta ttcgtgctat
     3661 tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag aagggccagc
     3721 tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg ttacttcctc
     3781 tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg ctgttgccca
     3841 tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg ataacttcaa
     3901 tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg cagaaatcga
     3961 agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg taggaaaagc
     4021 catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg atatagatgt
     4081 cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt gtcaaaaacg
     4141 aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc gatgcattgt
     4201 ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc ctgacattcg
     4261 gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag aaaatattca
     4321 attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa ggtgatatct
     4381 ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg gataaagcaa
     4441 cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat gctccagttt
     4501 gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa agtcgcgata
     4561 agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg cggaaatacg
     4621 catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat gaccactgat
     4681 catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag cctttggatt
     4741 tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca atttaggtaa
     4801 tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata cactgataga
     4861 gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg tagacgcatc
     4921 tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt cttcttcatc
     4981 tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga tacctcgtga
     5041 ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta aagtcttttg
     5101 ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta agatcaatgg
     5161 ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct ctccttttct
     5221 ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat cttcaatctt
     5281 ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc aataagggca
     5341 gacgactggc atttctttat cattatgttc gactacatta ttgacttctt cgtatgcaaa
     5401 tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc ctttcatctt
     5461 tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc ttctatgcag
     5521 atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa taaggacgtt
     5581 catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag tagctgactg
     5641 cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata tcaaaaagat
     5701 gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat ggattgactg
     5761 ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta ggattctcca
     5821 aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt tcgtttttta
     5881 cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata tcatccatgt
     5941 gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg agggcagcaa
     6001 caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa agaatatcaa
     6061 gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt gttatcttta
     6121 ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt tttttcctgt
     6181 aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt ctgcaattgt
     6241 ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt ctggatagtt
     6301 tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc ctagtttcag
     6361 atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt caaatcctaa
     6421 tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg ctatatgatg
     6481 gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga tctatattaa
     6541 tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc ctttttataa
     6601 gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac actcttcctg ttcccaatat
     6661 aataaatgaa agtaagaaat tatattttca agaaaacaaa cagaaaatta tcataacaca
     6721 aattatttat aattgaaaga ctagtttttt ttcaagaaaa gcatacaata gattgtgtta
     6781 gtaaatacag aacacactac atttagtgtc ttaaggagtc ctcatacatg aagtcatatt
     6841 ttcagttttt agctcatcag ctaaaaggct ggccgcaaca aaattattat cttttctttt
     6901 tcagtcttgg ctgccaggtc atgactcttg tcaatagtcc catcacttgg ctgtccgtta
     6961 ttacatttat cggtaccacg cttggtgtat tgtgtatttt atccatcaat gcggcaaaat
     7021 cagtcaacgg tattttaggt attttgtctg cgatttgttt tattatcgtt gggttttcag
     7081 caaagaacta tttaagtatc ggtgaacaga ttgcttatat gatcacattg gatatccccg
     7141 tattgttaag taaagaatgg aatcataata tggcttcaaa aatccgaaaa ttcactggaa
     7201 aaagctggtt tatcgcaatc atcagtactt tccttgtcta tcttgtttca ggattcctga
     7261 ttggtcgtta tacagatgat cctcggccgt gggtagatgc cattgctttt tctattagct
     7321 tgactgctgg tatcatcagt ttcttgcgtt acaacaacca atattactgg tggttagctt
     7381 cagggcttgc tcaaatggtc ctttggtata tttcatttac gcatggatct gcaagtttgg
     7441 cgatgttcgt gaacagctct gtttacttgc tgaatgatgt ccttgcattt accgtttcac
     7501 cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa tactacaacg
     7561 cacaacaaca cgcataaaaa aactgagcgt gggacaaaag taaagaacac ttttgtctca
     7621 cgctctaaac acgtataaac ggcggaatca gaagcaactc cttcggaaat aagctgaaat
     7681 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     7741 tctgtcccag cctctatttt ttatagtaat tccttcagat actttcttgc ctcatcattg
     7801 aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg agcttcagta
     7861 aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc agattggata
     7921 tctttcacta ctagttgcca tttttctttt gagactgtac ggtctttctt atcagcagat
     7981 tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac tatccgagga
     8041 atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca aataaataga
     8101 tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg agcagtgact
     8161 tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc aaaataagca
     8221 ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg taaaaatgcg
     8281 cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa gtcgtcaaat
     8341 agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact tattttcttg
     8401 atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac ttggcgaaat
     8461 tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat ccgacgattt
     8521 tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat attcttctgc
     8581 ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg atggatcgaa
     8641 gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc tagttttagg
     8701 atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct accacactca
     8761 caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt gaagtcagaa
     8821 aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct gattagatat
     8881 cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata gatctcccaa
     8941 cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct gatttctttt
     9001 ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt cctttctaat
     9061 tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt gctaaattag
     9121 tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc ttcaactgca
     9181 taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac tggacgatcg
     9241 atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc cgggaattgt
     9301 gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt tcctgctgta
     9361 taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc tttgattggc
     9421 aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa tggctgattt
     9481 ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc gaacgagact
     9541 gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg ctgttggatg
     9601 gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac tttgatgatt
     9661 tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg attcactttg
     9721 tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc ctccgtccga
     9781 ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg taaataaagg
     9841 aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa aaaaatagtg
     9901 ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc tctcttctat
     9961 tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc aataaacaac
    10021 tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg cacgatattg
    10081 aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa tgcaaccatt
    10141 cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat cccaaccgtc
    10201 atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac catgccaaga
    10261 gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc tactgcattg
    10321 gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc taaaagcatt
    10381 ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc taaaaatgta
    10441 cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa aacaccgacg
    10501 cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc aaagacaccg
    10561 atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt ttccatatat
    10621 tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc tggtatggtc
    10681 atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc tgcaataact
    10741 acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt tttatctgtt
    10801 aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag gatctccagt
    10861 aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt gcccctttcc
    10921 tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac ttgccacaac
    10981 taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg ttacgtcata
    11041 tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga tgaatagtac
    11101 ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta gttcttcttc
    11161 atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca tcggtaaaat
    11221 gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg caaagatcat
    11281 tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga atcgcttgat
    11341 caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg ccaacataat
    11401 gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa gttctaacat
    11461 tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca ttacgatcaa
    11521 tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta aaaggctaag
    11581 ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta taaagcaaaa
    11641 cacataaaaa atatagttgt tttctcacga aatagacact atttttaaat atttttcgtt
    11701 atttttttat cacaaaaatt atcttactta ttgtaaggta aagctttttt taatttattg
    11761 cccgataata tatagaaata aggtctagtt actgtttttt ttaaatgatt aataaataac
    11821 aacttctttc tatgaaattg caaagcattc atcttctttt ccgctaacga tatatccgtc
    11881 agtgcttggt acactttact aaaaagtatg agattgccga tatctggata ggtgtgctat
    11941 actacagaaa aagatataca atctgccacc gggtaggata aagaggatga tcattccatt
    12001 aggtctttga agggatgatt ttcctctttt tgtgtatttt tttatagtta aggaggaatg
    12061 taagtatggc atggatggaa ttaatcatag caggaatttt ggaagtattc tggtcaacga
    12121 tgatgaaatg gagtgatggt ttttctaaaa tcaattattc tttttacaca gtgattggca
    12181 tgattgctag tttttacttt ttgtcaaagg caatcaaaag tttgccgatg agtctctctt
    12241 atcctatctg gacagggatt ggcgcagttg gttcggtcat aattggtgtg gtcttgtttc
    12301 atgataagct gaatctttcc acttggttct ttgtggggat gctactcatc agtatcattg
    12361 gcatcaagat tactagcggc cactagacac ttggaataag tactgatcag aaaaaataag
    12421 cctttttatt taatattcaa tttttttatg aaaaaataag aaataagtcc aatgacctag
    12481 cgacttctcc actcatcagg tgtatgcttg tactatctat agaaaaggag tgaatatttt
    12541 tgaaaagtct ttcggtccaa gaattatcca agaactttgg tcacaaacaa gccctcagcg
    12601 atgtcagttt tagcttatcc cctggcaaaa tcgttggatt agtaggtcct aatggcgctg
    12661 gaaaaacaac gatcatgaaa gctattttag gactgatcca ttactcagat ggaacaatct
    12721 gtattggtga aaatgagatt tctccctcta gttataaagg gttggaacaa gtaggagcat
    12781 tgatcgaata cccgggtatc tatccattct taacaggcta tgatcatctg aagttattct
    12841 ctgaaaccaa cgatgtatct gctatcgaca ctatcgtgaa tcagctcaag atggaaaagt
    12901 acattcacaa aaaagcgaaa tcctactcgt tgggaatgaa acaaaaattg ggaattgctc
    12961 ttgccttact gaaccatccg tccatcgtta ttttagatga gccgatgaac ggtttggatc
    13021 cacaagcaac aagagatgtg cgaaaaatca ttaccgactt ggctactcag ggcacctctt
    13081 tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag ctgatcctga
    13141 tcaatcaagg aaagatcatc aaacaatgta caatggctga attgaaccaa acagcgcaag
    13201 atttcatcgt tatctctaca acaaatgatc tagctgctcg aacgatcttg acagaagctg
    13261 gatatacttt ggaagatcct gataaaatca agcttttaaa agaaaacgaa gaattattag
    13321 gaaccgtgat ccagctattg tctgcacaaa atatccatgt gacagacgtc cagcatctga
    13381 cgaatgattt ggaatcttct ctattggaaa ttctggattc aataaagggg gaagaagaaa
    13441 aatgacattg cttgtaaaac aagaattatt taaattgatc aagaaaaagt caactgctgt
    13501 tttatctgtt ctattagttg ttttgctcat aggaacagca cttctagcca aaaaatacac
    13561 gacgatcatt gatccagttg aaatgactgc tcagcttttt agtgccacat cttggattgt
    13621 ctttatcatg attgcagctg caagcacgat catttcgatg gaagcacaat atggtacatt
    13681 gaaaaatctc ctttatcgaa agtattctcg aggagaaata ttagtaagca aatggatcac
    13741 attagttatt tattctgttt atttatacct tttagctatt attgtcactg tgttgatgaa
    13801 actgattctt ttcccttcga tttccttcac ggagaaggtt tctactggtc aaacactgat
    13861 ccaaagcttg ttcacttata ctttgggtag ttatatcggt ttatggctga ttttaagttt
    13921 agtgttgatg attgcttgct ttatcaatag ttccggcgca tccatctcag caggaatcgt
    13981 cttttatttt gcttcgtcaa tcatatctgg tatccttatt gcactcatcc aaaaatggga
    14041 gtggatcaaa tggaatccga tcagtatgtt gaatctgcaa aaccaaattg gaaacgaaga
    14101 gatcatgaag caattgactc atctttctac taatcaaatg ctattcggta atttagctta
    14161 catcgttttg ttcttagcat taggatatct cgtatttaag cgaaaaaatg tatgatgact
    14221 catcaaaaaa gaggctgtga aagagacgtt cagccttatt gccgattggc tgtctcttaa
    14281 acaccgttta ttctgaaaaa ggagctgtga caacatttgt gtcacagctc ctttcctagt
    14341 atgtttttga ataaacgaca ggctcacaac ctctctttat acttctttta tacttctata
    14401 cgtgaagcat cccagtggca aataaaacca ttgcaatcac tccgaccatc gtcacaccta
    14461 taagtaaaat cttatctaag tggatatgtt gtacaggctc gttttgctcc ttacgtgctt
    14521 tgaggtaaaa ataaatgcct ggcaagaaaa gaacaagaac aagcattact tcattaaagg
    14581 ctacgactag catagcccat acttgaaagg cacttgctaa aagccctact aaaaattgga
    14641 cccattcttt ccgctgataa gaaagtttca tttggtacaa tcctaagaaa agccacgtga
    14701 agaaaatagc tgctgtacca agtgtgtaag caaagttata agcactgtca gtgaataata
    14761 atgaaagtaa aaagacattc gtacataacg tcgtcaatac gatagcataa gttggtgccc
    14821 catttttgtt gagttttccc catgctttag gcaaaagtcc gtcttcagcc atgtttccca
    14881 ttgtttcaat cggtaagatc gtccatgaga gccaaacacc aactaaagaa atgatcaaac
    14941 cgccgttgat cacgactgct ccccatttac caaccatcat ttcaaacaca tagcccatag
    15001 caggctgcgc cttgatcgtt tgcagctgat tcatcgtcat tgtcccatat ggaagcatgg
    15061 aaaccaagac gtaaataata atcaagccga atatccctag aatcgttgct ttcccaacat
    15121 ctgattgttt ttttgctcgt gaacctacga cagaggctcc ttcaatacca acaaataccc
    15181 aaagcatcac catgatgcaa ctcaatactt gactggaaaa cgggatcggc tgtccattcg
    15241 cattcaatat aatgtcgcta gaaaagttat caatgaattg ttggatcgaa aacgtgctga
    15301 tagctactac aataaataaa aataatggca ctaatttaca gaatgtgacg atagcattga
    15361 cgaaactagc attttcgact ccccgattca ctagccatgc atatccccat aaaaagactg
    15421 tactcatcac gatcgatgcc agattttgcc caccttcaaa tactgggaag aaatatccta
    15481 atgcgctcat caacaatgtt gcaaaagcta cgttgcctaa ccagcatgtg agccagtaac
    15541 cccaaccgct gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa ctaaagatcc
    15601 cgccttttaa atctggacgt tttttattga tattattgat cgacaaggag agcatcaaca
    15661 ttgctgttcc tacgagtacc caactgaaaa tagctgggcc gccagccgat ccggagatgt
    15721 ccgtcataat accaaatacg ccacctccaa tcgctgaacc aattactaag gcagcaagtg
    15781 gaattaaacc aattcctttt ttctgttgct ctgtatccat gtaagcatcc tccaaaataa
    15841 aaccgctttt atcaaaaagt ataacaaacc ttttttattt ttacttaatt aataaaaact
    15901 ttaatagaaa tataattcta ccggcttttt tctcattata caagcaaagc gcctagtccc
    15961 tttccaaatt acaccaccaa aaaacttaaa aaagatgcta tactaaaagg aaagaattgg
    16021 aggatgatcc atgaacattt tatatatatc gatttcagga aatacgcgtg cctttgccaa
    16081 acatctagca gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc ctgaagttac
    16141 tttaaaagaa atccatgaaa atagtgattt tacgaaagaa acagaaccat tttttacttt
    16201 cgttcctact tatttagacg gcggaaatgg tttagataac ggtgatacag aaatcttgac
    16261 cgaaacgatg cgtgaatatc tggaacatga agacaatcat aaactttgtc ttggtgtagt
    16321 cggtagcgga aataaaaact tcaataatca gtattgtcta acagccaaac aatatgcaca
    16381 aacatttggc tttcctttct tggcagatta tgaattacgg ggaacaccat ctgatgtgac
    16441 acgaatttat cagattttag cagaaaacaa taaataataa aaaagaagaa gctgaaaaat
    16501 agatttcagt ttcttctttc gtttctattg tttctatcat attaagcttt gacatccatc
    16561 acaggtgctt ttgcttttgc tttacctgtt ttttccaaga caaagttttt tacacgatcc
    16621 atattggtta ccaagacgat catggatgtt tctttgcctg ctgctttgat cgcttctaag
    16681 tcagcttctg ctacaagcgt atcaggtgtc acttgctgcc cttctgttac tttcacatca
    16741 aatggcgtac cgcctaagtc aactgtgttg attcccatat gaagcaagat ttctaaacca
    16801 tttggcgttg tgatcccaat agcgtgtttc gttgggaaga cagagctgac taccccagta
    16861 actggagaat agatatgtcc gttttctggt tctacaaagt agccttcacc catcatcttt
    16921 tgtgcaaaaa ctggatcact tgctttatct aaagaaacaa ctgttccgtc agcaactgat
    16981 gagaggatag tcacactttc agctgaagtt tgaggtacta ctttttcagg cacttcattt
    17041 actgtggctg tttcagtcgc taattcttct ttttctaaac ggtctttagg aataccgaag
    17101 aagaatgtag caacgaagcc accagcataa gctgccaata atccaaggat gtagcctaac
    17161 catttaccgt cagagatcaa tgggatcaaa gcggctcctg aaggtccaat agcgatagcc
    17221 ccgacatttc cgatcattcc gatcactgcc ccgccgatac ctccaccgat acaagcagta
    17281 ataaatgggc gtcctagagg taatgttact ccgtaaatca atggttcgcc gattcctagg
    17341 atcccgactg gtaaagctcc tttgatcatt tctgttaatt ctttatcttt tttgcagcga
    17401 atccataatg ccaatgctgc acccacttgt cctgcaccag ccatcgcaag aattggaagc
    17461 aatagcgtac gaccagtttc atcaatcatt tgcacatgga ttggagtcaa aatttgatgc
    17521 agtccgaaca taaccatcgg caagaataat gtgcctagaa cgaagccggc aaatgctcca
    17581 ccaacgccaa gtacccagtt gatcccaccg accatgccat cagaaatgaa acctgctaaa
    17641 ggcatgatga agaaaatttc aatcagaccg atggcaatca atgaaagagt tggtgtaacg
    17701 atgatatcta ccgcatcagg tactacttta tgcagttttt tctcaacgat tgacaatagc
    17761 caaacagcga agatgacacc taggatccca ccttgtccag ctgcaagagc ttcaccagta
    17821 aataagtttt tcagtggtgc ttccggattc atccctgtca gcatcacaac ggccccgata
    17881 actccaccaa gagttggtgt tgcaccaaaa acttgtgcag cattgatacc tgtatagata
    17941 actaggtagc taaacatccc gtttttcaag atgttcatca catcgatata ttgttgccat
    18001 gttgctgcat caagtgttcc ggctgtgact aagtttgcta aaactgcggc aatccctgcc
    18061 acgatcccag taccgacaaa tgcaggaatc attggcacaa agatattaga aatgtctttt
    18121 aataccgatt tgattttaga tggtttttgt ttcgattttt gtgccgcttt catttcttgt
    18181 gcccgttcgt ttacacggtc ttttccagat gctgatgaat ttgctgtagc tgtacttaca
    18241 gaagaaagtt tttctcctaa tttcacgcct gccaaatcaa ccatttgctg tgctactttg
    18301 ttcactttcc caggtccgat gatgacttga agctgttcat cttctactac accaagcact
    18361 cctggaatcg cttttaattc agagacattt acaagatcat cattgatgat cttcatacgc
    18421 acacgagtca tgcagtggac aatgctttct acattgccca tgccgcctgc ccctttataa
    18481 atttgttttg ccagttcttc caaagtcagt tcgttttttg ccatggtctt ttccccctca
    18541 taaggtccct tttacaaatc cgtttgcttc agctaatttt gcttgagctt cctctttatc
    18601 actatttgtc aaaatcatga cgatagccag cttaacattt tgatctgctt cttcaaagta
    18661 cttttcagct gtttcgtaat cagcttctgt cgcttgcatg atgattcgtt ttgagcgttc
    18721 gattagtttt tcatttgttg gtttgacatc gaccatcaag ttattgaata ctttacctat
    18781 accaatcata ctgatagtag aaatcatatt caagataagt ttttgtgctg ttcctgactt
    18841 caatcgagta gagcctgtca aaaattctgg acctgcatcg acttcgattg ggaaatccgc
    18901 gtgtttactg atttcggctt gtttattgca agaaatcgtt cctgtgacag caccgatcga
    18961 ttttgcgtaa tctaatccac cgatcacata tggtgtacga ccgctagctg cgataccaat
    19021 aactatatca ttttttgtca aattcagctt gattagatct tcttgcccta gctcataaga
    19081 atcttcggcc ccttcgacag ctacagtcat tgcttcttct ccaccagcaa ttagacctat
    19141 gaccatttcc ggttctacac cgaatgtcgg tacgcattca gcagcatcta acacacctag
    19201 tcttccactt gttccggctc ccatataaat cagacgtcca ccttttttaa aggactcaat
    19261 cgtgttagca atcactggtt cgatacttgt cagttctttt tcaaccgcag cggccacttt
    19321 ctgatcttct ttgttcatta gttgaacggc ttgagaaata ctcatctcat ctaatccaaa
    19381 agtttcttgg tttctgcgtt ccgttgttag gttctctaaa ttgattttgc tcatttttat
    19441 cttccttctt ttcttataaa ttgatagttc ataccagctt tgatttggtt gatcaatggt
    19501 agctcttctt tgataacttt agctactaca ttcacttttt catccgctgg caaatcatat
    19561 ttggttaatt ggatctcacc catatagcgc aaatactttt cgttatctaa agtaatagac
    19621 cctttcatac gcgtagctgt atttcttgct gggataggcg gaatctctcg aaaacgtgca
    19681 tctacgcttc gtatgacatc tcttgcatcg tcttgccgat tcgtatgctt ccctaatata
    19741 tattcgtaaa attcttcatc tacaggttcc gtatggagca agactgtctg ttctttttgg
    19801 aaagaagcaa attgctcctg aacgttttct gacagacctg catctccgat gtaaacaata
    19861 tccgtgttac ttgctaatag atctagagcg cctgcaagag gatggatccc tcgatgttct
    19921 tccaaagttg gcaatccttg atatagtggg cctctcaact ctgtatctcc tggtacaaaa
    19981 ccttggacaa ggagaccttg aattttgagc cattgatttt ttttctgata ccattctttg
    20041 tccagccctg tttcgggacg tggataataa ttatgccaag cttcaatctg tgagaaatca
    20101 gcttctgctt ctttcaattc atcgatatct ttaggcgtga tcgtgctagc attcaaactg
    20161 attttcaatt gatgtgacca gtcggcaatc tgctgattgg aaatatgata atccattctt
    20221 aaacctgtta caccaattgc tttcaaaggc gttagatctt ctaatgaaaa acccgctcgt
    20281 tccaatgcct ctcctgaaat atcgaccatc aattctagtt tattcttttt cgcttcgctt
    20341 cctagttcga tcaatcgctt ttgatatgcc gaaacatcat cttctggtat atgcatggaa
    20401 gtgaaaatac cgacaaagcc attggatgcc atttggcgga tatactgttt cttctcttct
    20461 gacaaagaat cgttcataaa tattgaaaag ccaaacatat tatccctctt tttctgtatc
    20521 cgtcttcatt tctatgacta gagtatatat ctaaagaaac aaaatttcaa tacttttatt
    20581 aaaataatta aatttcattt ctaaaggttg agtttatatt atcaaaagaa ttgtcataac
    20641 ctaaaaaagg cactgctaag caaaagcagt gccttgaaat attttattta aataacacaa
    20701 aaacataatt actcataaga aaccaattct gtattatcta ccatgaatgt aaagaacccc
    20761 tcattaggca tcggatgaaa ataagcattg atcactctta tcaaggcatt atggcaaact
    20821 aacagataga cattttcttc gtcttcgatg cattctttca ataaaggaac gatccgtgca
    20881 tacacatcta agactgactc cccatttggg aatcgtacag cgaacgctaa acgtgctttt
    20941 tgaaagttct catctttgct tggcagtcca tcataatccc caaaatccat ttctaccaat
    21001 cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc tgtatctctt
    21061 gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc tactaccttt
    21121 tctgctagtt tctgtgcctg catctgtcct ttttcagtca gcggcacatc tgcccgtccg
    21181 caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata aagcatgtga
    21241 ttctctcctt cgttggcaaa gttcccctta accatacctt gttgttcttg agattacaag
    21301 ggttagattc tctcaagaga aataatctca gtagatactt tatccaggaa ggtttgatca
    21361 tgttccacta gtaacatggc aggtttcacg ttcaagatca gttgttctaa ttgttcttga
    21421 ttgaagacat ccaaataatt caatggttca tcccatgtat atagttcagc tggctgtgat
    21481 aaagatttag ccaattccac ttttttccgt tggcccatac tcatctgctc gatcttgtta
    21541 tgaaaaacat ctctttccat cccaagcttt cggaggttgt tcaaaaatgc ttggtagtct
    21601 acttggtttt tctctgcaaa ttccgccaac gttcctcgat tgtcttcata attttggctt
    21661 gcataactaa tgctcagatg ttttggctgg tatttttctc ctataacctt gccgttgaat
    21721 gcccccagaa gataatggat aatggatgac tttcctgcac cgttaggacc tgaaatcgcc
    21781 acccgctgat gaggctcgat tgtaaaatga attggctcga ataacagatt ttcataccct
    21841 aattgaagat cttctacgct taaaagtcgc ttatggtgag acgcttggct attcatcgtc
    21901 aaagaatcga tatattcgat atcttttaat agtttttctt tctcgctgat ctgggtctcc
    21961 atccgattca cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc acccactgct
    22021 cctttattca ctcgtctaga ttcagtatcg atgaatccga cttgtttctt tgttttatct
    22081 ccttctcggg aacgagacca ttcggctttt tcagctgctg ttttctttag cctactgact
    22141 tcttttttca atttttcatt ttgagccatt tcaaattcat cacgaagttt tttctgttct
    22201 tcatagatag agaaattccc ttgataaagt tccagttgac ttttttcgat tgctaaaaca
    22261 tggtccacta cttcatcgat aaatccccgg tcatggctga ccacgatgaa gccttgtttt
    22321 ttctttttca aataagccgc tacttgtttt ctaccagaga tatccaaatg attcgttggt
    22381 tcatcgatta acgggaaatg agtgtcatcc acaaataaaa gtgccagtag gacttttgtc
    22441 ttctctcccc ccgatagtgt gctgaattct ctccataaga tttcaggatc tgtttgcatc
    22501 aattggagct ctctttcgat ttcccatatc tcaaaatccg taatatcatt taacacgtaa
    22561 taggttaaac gttctttatc ttttgtctgt tgcgggaaat aggcaaattc ttgctgatgg
    22621 attacttgcc cttgataagg tagtttgttt tgtagaatat tcagtaaagt tgtctttcct
    22681 cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaaatttag atttgcttgt
    22741 tcaaataata atgtgccttg gctgtcgtag ccgaatgtca gattttttat ttcgatttta
    22801 gacattttcc atcacctcgt aaaaaaagac cagtcctatt ttcagaactg gctaaagagg
    22861 atgcaagggt tggatacaca caaaaagacc catacatcga aaaaaccaag ccaatcccga
    22921 aaatctgcac actgcttcct tgtctccaca aggtacagtc tgaatgcagc tttcatcaat
    22981 cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa ttacgatagc
    23041 ataaagaaaa tgattttgtc aactacttcg ggaggatggt gaaaaatctg ctaagtgtct
    23101 gtcctaagat ttttgttttt tctaatgttt gcctgtgttc cattgcttct tctaaaccta
    23161 ttggacccaa atgaatactt aagacaattg ggagaaaatc agaaagtcct tctagctctt
    23221 ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca agcactgcta
    23281 caccgtatgg aacttttccg tggatcgttt gctgatcgat ccgcccttct cctgtaatga
    23341 tgacatctgc ttgttcgatc tttttttcca atccagtgat ttggctgact aaatcgaaac
    23401 cagaaaccat cttcgctccc aacaaaaaaa gggctccacc aataccgcct gctgctccac
    23461 ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga gcaatctttc
    23521 ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt cttccaaatt
    23581 gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg ataatcaatt
    23641 ttactttaga aacaagaacg ttttttcgat caaaatgaag ttttttagta gataataaag
    23701 gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat cctagaccac
    23761 catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct tgaagcagtg
    23821 catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt tgatccgttg
    23881 gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg gtgttttttc
    23941 cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga tccatcccat
    24001 caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta ttttctccgc
    24061 catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca tttagaactg
    24121 cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc gccaaaatat
    24181 ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt tttcaaacaa
    24241 aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg aataaaggag
    24301 agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg aatcggattg
    24361 atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc tcacattgaa
    24421 gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt cgcaaaactg
    24481 ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt gatcgtagca
    24541 ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat tattactgac
    24601 aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact ggaaaaatat
    24661 gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct agctggtccc
    24721 ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga accagaaggc
    24781 gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa tccgatctat
    24841 agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac attgaatggt
    24901 ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc ggcagagagc
    24961 atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg aatcatacta
    25021 gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa catcgggttg
    25081 cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga aatcgactac
    25141 atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc atattgtgcc
    25201 tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata aaaggctctc
    25261 tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat aaaagatcaa
    25321 aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc agtacataat
    25381 ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata ttcgtattgc
    25441 tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc atcataaaat
    25501 tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg taattcatct
    25561 aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata atgagcaatc
    25621 gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt ccgcaaagtc
    25681 actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc tgtttttttc
    25741 aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg aagaatgaaa
    25801 tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc tcgttctttt
    25861 ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaattc
    25921 atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc ttccaaaaaa
    25981 tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg
    26041 tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt aaatgggcgc
    26101 acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt
    26161 gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa
    26221 acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc aactagcagt
    26281 acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc tatgatagcc
    26341 tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa tttaacattt
    26401 gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa agttttctga
    26461 atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt gctatttatt
    26521 cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga agataaaaaa
    26581 ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg agcaatcggt
    26641 gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt
    26701 gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt
    26761 ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct
    26821 cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct
    26881 gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta
    26941 tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc
    27001 gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt
    27061 tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag
    27121 caattgactg accatggtgg gtttactcct catggtatga agggaatcat cagttctttg
    27181 cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca
    27241 gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc
    27301 ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc
    27361 acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat
    27421 atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca
    27481 tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct
    27541 gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat
    27601 cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc
    27661 ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca
    27721 ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga
    27781 ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt gactggcctg
    27841 ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt attgacgaat
    27901 gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc tattgaagaa
    27961 aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt ctcatgacct
    28021 cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct ccatgctcat
    28081 gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa agtattggaa
    28141 gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt acttgaatgt
    28201 ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt tgatgatggg
    28261 tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa acaaaaagat
    28321 aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt ttatggtacg
    28381 aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc atcattctat
    28441 cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga
    28501 agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga tcaaagaaat
    28561 ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta tcagcttcaa
    28621 gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa aagctgccct
    28681 gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg tttggttagg
    28741 cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta aaatcagcgt
    28801 tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt gccatttttt
    28861 gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt gaaaaagtcg
    28921 cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt tgtataaatt
    28981 tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg gattgtgaca
    29041 aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg tcttgcttac
    29101 tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg cactgaccat
    29161 cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga gtgtaggaat
    29221 accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt agttgattac
    29281 tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg ctttttcata
    29341 agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac caataaaaat
    29401 atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt tcgtcatctt
    29461 tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca atccacctaa
    29521 tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc ctccgcggct
    29581 aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt cgatgacccc
    29641 agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta ctgctacagc
    29701 gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca gtaacattaa
    29761 aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa catcagtcac
    29821 ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa
    29881 gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt gtttgaacaa
    29941 atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag acatcttatc
    30001 tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta agcctagtgt
    30061 tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac
    30121 agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact ggacactgat
    30181 caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc atacagcaat
    30241 caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga tgccattgat
    30301 gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca acatgaccat
    30361 tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa cgccaatcgc
    30421 aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg gtgttccttt
    30481 ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat
    30541 cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa aagacctgtt
    30601 ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca
    30661 ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc
    30721 ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc gtgaaaagtt
    30781 gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt
    30841 catttttctg aaaattattt ttataacttt cacacacaag tttatttttt actagaaagt
    30901 agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga ttctttctaa
    30961 tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg ctgcaatcgc
    31021 aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg tgtgtccggc
    31081 aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt ggctgacatt
    31141 tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt catctggaac
    31201 acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa cgatgacatc
    31261 atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta tcgcagcacc
    31321 tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat ttcctgcacg
    31381 taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc ctccgtctgt
    31441 aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt tgattccatt
    31501 aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg
    31561 ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt ctgtcgtttt
    31621 cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa aaaaaccttc
    31681 tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg gggtgccata
    31741 tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg cggctaatac
    31801 ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta aagcatcgaa
    31861 ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg catcaaaggc
    31921 tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta attgtttaat
    31981 caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt ggatatctag
    32041 agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt ggtttgttga
    32101 gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt ttctcccgaa
    32161 tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt tgactgtgtc
    32221 ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat cttcttttct
    32281 tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta agaagatggc
    32341 atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca ctgaaatgtc
    32401 tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt ctagattttt
    32461 tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat ccactttgat
    32521 caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat cattagggat
    32581 tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag aataaattcc
    32641 cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt ctttattgta
    32701 attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat ctgctaaagc
    32761 cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt tctccttttt
    32821 caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc ctagttttac
    32881 gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg ccaaccaaat
    32941 aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt ctcctccact
    33001 ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa aaaactaaga
    33061 ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta taccctctcg
    33121 atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta gtcagtccga
    33181 tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct tctaacaaat
    33241 acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct tcgatcaccc
    33301 caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga acaactccaa
    33361 aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag aaacgagtag
    33421 tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata ttgatcagct
    33481 tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt gtcactacta
    33541 acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa aaagacatat
    33601 atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca ttgggcaaat
    33661 atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat acgactatct
    33721 aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata atccaatgct
    33781 tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa cggcgtttct
    33841 tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt gctgtacaga
    33901 tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac cagccattta
    33961 ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc ttggtcaaga
    34021 tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct gaagatctgg
    34081 atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt gttgcagatt
    34141 tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa ctggcgattc
    34201 aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc actggttgct
    34261 tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc tactcgttgt
    34321 ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa accgacaagt
    34381 tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga atactttaaa
    34441 gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg ctgaaagatc
    34501 ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact cagattcgtc
    34561 tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac acgaactaac
    34621 gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt ttcaatcgtt
    34681 aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaaca
    34741 tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc aagaaaatga
    34801 aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc aagctgtgtt
    34861 cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc gcagccgtca
    34921 tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct
    34981 tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc
    35041 aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt cagtaaaagg
    35101 acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca
    35161 aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc tacttatcga
    35221 caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca ccagccagag
    35281 cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat tttgttgttg
    35341 ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt attttttcta
    35401 tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat cttttccttc
    35461 cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa aactggaatc
    35521 aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta ctaaagaatc
    35581 ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg tcagataagg
    35641 aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt ccaagtaatc
    35701 agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg tcgtagcaat
    35761 gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat atggttttaa
    35821 gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg ctcggacttc
    35881 ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt gtttccagtt
    35941 ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt gctgaaatcc
    36001 ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat ctaaatcaaa
    36061 tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag tttagcacga
    36121 ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag ttcttggaat
    36181 cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat gactgtagca
    36241 aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc accaaaactt
    36301 gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa catggcaaat
    36361 gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag cactaaggtt
    36421 tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa aatcactcgc
    36481 tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat aacaaaatac
    36541 atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta aaccatgaat
    36601 tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt atccatccga
    36661 tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa aagttgtaaa
    36721 ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa catattgcca
    36781 aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt ttctcgtcca
    36841 gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac gtctgcagat
    36901 tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac tttcattgca
    36961 ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt acgtaaagct
    37021 gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg gtcttcagct
    37081 acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac caattcattg
    37141 atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc cccagtcaga
    37201 acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt tgtttctttt
    37261 ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc tttgactgaa
    37321 aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg taagccatct
    37381 tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt taatggtgta
    37441 acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat cgcgcctttt
    37501 gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg acgacgttca
    37561 aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc taaagtttct
    37621 gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg atagtagcta
    37681 tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc catatgatat
    37741 tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa cacatcgatt
    37801 gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt ggccatcaca
    37861 cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg tgttagacca
    37921 actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt aagcccattg
    37981 atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat ggatgtttta
    38041 tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc tgctacatga
    38101 ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc actgatgaca
    38161 ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc gtcttcacat
    38221 actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg ggaaatgaat
    38281 aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc tgaaagccga
    38341 acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc attacgtttt
    38401 acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt ggaacgtacg
    38461 gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat ggctacaccg
    38521 aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga aataattcct
    38581 aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc tacaatgaat
    38641 ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga acgttcgatt
    38701 tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc ttgtttcgtt
    38761 tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat ctcaatctct
    38821 cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat tcatcggatc
    38881 gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg cctttcctct
    38941 agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta ctaagtaatt
    39001 ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga ccacctcctt
    39061 tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg
    39121 aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga gttttgacac
    39181 tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc ttatctcgac
    39241 aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat
    39301 aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca actaaaaaag
    39361 aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac acgttatatt
    39421 cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata gaaatatagt
    39481 aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga cggcgtgaga
    39541 cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa
    39601 atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca cttgactgag
    39661 cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt ttatcttgtg
    39721 ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac aaaagctgcc
    39781 gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga aaacatcatt
    39841 cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt cccgccattc
    39901 gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac tgctggtcta
    39961 gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt gattctttcc
    40021 taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt cttcccctaa
    40081 agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga tcactgtact
    40141 tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg cctttttcaa
    40201 ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga gatactgctc
    40261 atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat aattgttctc
    40321 aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt caaattgacc
    40381 atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac gtaaaaattc
    40441 aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa ccaaataata
    40501 cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat aaggatcaaa
    40561 gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta ttggttcatc
    40621 cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct ctaaaaatga
    40681 tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga ctagagaagt
    40741 cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga ctcgaagttt
    40801 cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt tttccacatt
    40861 caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc
    40921 ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg ttggtttgat
    40981 tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat ataagttggt
    41041 tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc
    41101 gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat caatcgtctc
    41161 aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat ctgaaagact
    41221 taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc ctgctgcttt
    41281 cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga cggtgtattt
    41341 gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag aagataataa
    41401 gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta gtaatgtcgg
    41461 caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat aaccaggcat
    41521 atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc atgtatctgg
    41581 atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc caaaatgatc
    41641 cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat acgtaaaaaa
    41701 ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta ctgtgctatt
    41761 gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt
    41821 ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga
    41881 ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg attcatcaaa
    41941 ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat ctctattcca
    42001 aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt gtccaaggat
    42061 cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg agccttgagg
    42121 taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc cctgtcctac
    42181 ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac tatttaaaaa
    42241 cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga aactttctaa
    42301 atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat ttggctctgc
    42361 ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg catgcgcgcg
    42421 aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat cgattcctag
    42481 atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc
    42541 gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag cgatagccat
    42601 cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc aaaccagtag
    42661 cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca tcaaagaaaa
    42721 ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt tgactgtaat
    42781 gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg tatttaaaat
    42841 atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca aagaaaggcc
    42901 aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact ggatcgtacc
    42961 ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa
    43021 aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta
    43081 tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga aaatttccta
    43141 aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt ccacttttca
    43201 cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa gtcttgtggt
    43261 gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg
    43321 ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct ttttcacaac
    43381 ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa tatttataaa
    43441 acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg gcaggctcaa
    43501 gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat gtcaggacgc
    43561 taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca
    43621 accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct cagagttaaa
    43681 cggcaggctc aagacctctt gata
//
LOCUS       DACETN010000011        43052 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DACETN010000011 DACETN010000000
VERSION     DACETN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.012"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            211..1080
                     /locus_tag="IXF98_000574"
     CDS             211..1080
                     /locus_tag="IXF98_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1554911.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIISSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="IXF98_000575"
     CDS             1289..2314
                     /locus_tag="IXF98_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1554912.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="IXF98_000576"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="IXF98_000576"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1554913.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="IXF98_000577"
     CDS             3397..5100
                     /locus_tag="IXF98_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554914.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSIIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="IXF98_000578"
     CDS             5102..7135
                     /locus_tag="IXF98_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1554915.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRCVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="IXF98_000579"
     CDS             7146..7586
                     /locus_tag="IXF98_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1554916.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="IXF98_000580"
     CDS             complement(7786..8913)
                     /locus_tag="IXF98_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1554917.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="IXF98_000581"
     CDS             complement(8916..9593)
                     /locus_tag="IXF98_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554918.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9831..11624
                     /locus_tag="IXF98_000582"
     CDS             9831..11624
                     /locus_tag="IXF98_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1554919.1"
                     /translation="MKRREGETKWNGRFPKNNISLKARVQKLGSTLSSMVMPNIGALI
                     AWGVLTALFIPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATM
                     GVIVGTDIPMMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLAL
                     LGFSAIGPVVDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLG
                     TEQVAEAGKSILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYF
                     PYVMMKPLLFLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILG
                     VIVAAAVSFGISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQK
                     IIFACDAGMGSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERA
                     KQKAPSARFVSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEI
                     LLVHDDRSGSATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQ
                     MAAPQSIHLSVNSMVNPQKYESIAQYLKQTA"
     gene            11639..12076
                     /locus_tag="IXF98_000583"
     CDS             11639..12076
                     /locus_tag="IXF98_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1554920.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12089..13240
                     /locus_tag="IXF98_000584"
     CDS             12089..13240
                     /locus_tag="IXF98_000584"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1554921.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13302..14000)
                     /locus_tag="IXF98_000585"
     CDS             complement(13302..14000)
                     /locus_tag="IXF98_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1554922.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13993..15345)
                     /locus_tag="IXF98_000586"
     CDS             complement(13993..15345)
                     /locus_tag="IXF98_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1554923.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15568..16968)
                     /locus_tag="IXF98_000587"
     CDS             complement(15568..16968)
                     /locus_tag="IXF98_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1554924.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17129..17866
                     /locus_tag="IXF98_000588"
     CDS             17129..17866
                     /locus_tag="IXF98_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1554925.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17991..18470
                     /locus_tag="IXF98_000589"
     CDS             17991..18470
                     /locus_tag="IXF98_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1554926.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18467..19234
                     /locus_tag="IXF98_000590"
     CDS             18467..19234
                     /locus_tag="IXF98_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554927.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19464..20033
                     /gene="lepB"
                     /locus_tag="IXF98_000591"
     CDS             19464..20033
                     /gene="lepB"
                     /locus_tag="IXF98_000591"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1554928.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20137..20739)
                     /locus_tag="IXF98_000592"
     CDS             complement(20137..20739)
                     /locus_tag="IXF98_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1554929.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20845..21314
                     /locus_tag="IXF98_000593"
                     /pseudo
     CDS             20845..21314
                     /locus_tag="IXF98_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21420..22004
                     /locus_tag="IXF98_000594"
     CDS             21420..22004
                     /locus_tag="IXF98_000594"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1554930.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22007..23080
                     /gene="prfA"
                     /locus_tag="IXF98_000595"
     CDS             22007..23080
                     /gene="prfA"
                     /locus_tag="IXF98_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1554931.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23073..23912
                     /gene="prmC"
                     /locus_tag="IXF98_000596"
     CDS             23073..23912
                     /gene="prmC"
                     /locus_tag="IXF98_000596"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1554932.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23905..24084
                     /locus_tag="IXF98_000597"
     CDS             23905..24084
                     /locus_tag="IXF98_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554933.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24106..25131
                     /locus_tag="IXF98_000598"
     CDS             24106..25131
                     /locus_tag="IXF98_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1554934.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25306..26550
                     /locus_tag="IXF98_000599"
     CDS             25306..26550
                     /locus_tag="IXF98_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1554935.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26703..27650
                     /locus_tag="IXF98_000600"
     CDS             26703..27650
                     /locus_tag="IXF98_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1554936.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27784..28413
                     /gene="upp"
                     /locus_tag="IXF98_000601"
     CDS             27784..28413
                     /gene="upp"
                     /locus_tag="IXF98_000601"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1554937.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28552..28794)
                     /locus_tag="IXF98_000602"
     CDS             complement(28552..28794)
                     /locus_tag="IXF98_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554938.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28915..28987
                     /locus_tag="IXF98_000603"
     tRNA            28915..28987
                     /locus_tag="IXF98_000603"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28948..28950,aa:Thr,seq:cgt)
     gene            complement(29106..29744)
                     /locus_tag="IXF98_000604"
     CDS             complement(29106..29744)
                     /locus_tag="IXF98_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1554939.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29867..30499)
                     /locus_tag="IXF98_000605"
     CDS             complement(29867..30499)
                     /locus_tag="IXF98_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1554940.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30850..31149
                     /locus_tag="IXF98_000606"
     CDS             30850..31149
                     /locus_tag="IXF98_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1554941.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31644..33809
                     /gene="acm"
                     /locus_tag="IXF98_000607"
     CDS             31644..33809
                     /gene="acm"
                     /locus_tag="IXF98_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1554942.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34081..34566)
                     /locus_tag="IXF98_000608"
     CDS             complement(34081..34566)
                     /locus_tag="IXF98_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1554943.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            35046..35687
                     /locus_tag="IXF98_000609"
     CDS             35046..35687
                     /locus_tag="IXF98_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1554944.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35706..36341
                     /locus_tag="IXF98_000610"
     CDS             35706..36341
                     /locus_tag="IXF98_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554945.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36359..37174
                     /locus_tag="IXF98_000611"
     CDS             36359..37174
                     /locus_tag="IXF98_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1554946.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            37493..38929
                     /locus_tag="IXF98_000612"
     CDS             37493..38929
                     /locus_tag="IXF98_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1554947.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            39118..41565
                     /locus_tag="IXF98_000613"
     CDS             39118..41565
                     /locus_tag="IXF98_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1554948.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            41795..42862
                     /gene="rlmN"
                     /locus_tag="IXF98_000614"
     CDS             41795..42862
                     /gene="rlmN"
                     /locus_tag="IXF98_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1554949.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT        14781 a         7362 c         8816 g        12093 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatctcta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccatcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gatgcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaaa caacatttcg ctaaaagcga
     9901 gggtacaaaa attaggcagt actttatcaa gcatggtcat gccaaatatt ggagcactga
     9961 ttgcctgggg tgtattaacg gctctattta tcccagatgg ttatttacca aatgaggctt
    10021 tcgctaccat ggtaagccca atgctaactt atctgattcc tttattgatc ggttatactg
    10081 gcggaaaagt aattgctggt gaccgcggat cagttgttgg ggcaattgca acgatgggtg
    10141 tgattgtcgg aacagatatt ccaatgatgc taggcgcgat gatcatggga cctcttggtg
    10201 gatatgccat caagaaattt gatcagctat tccaaaaacg gatcaaatcc ggttttgaaa
    10261 tgctagtcaa taatttttca gcaggtctta tcggttttgg attagcgtta ttaggttttt
    10321 ctgcgattgg tccagttgtc gatgcattga ctcaagcaat ggcaaaaggt gtagagatca
    10381 ttttatctgc ccatttgatt ccattgacaa gtatttttat tgaaccagca aaaatcttat
    10441 ttttgaataa tgcaatcaat catgggattt tgacaccatt gggaactgaa caagtagcag
    10501 aggctggaaa atctattcta ttcttgcttg aagcaaatcc tggtccagga cttggcgtat
    10561 tacttgcttt tactttgttt ggcaaaggag cagcaaaatc ttcagcacca ggagccatga
    10621 tcattcactt tttaggcggg atccatgaaa tctattttcc ttacgttatg atgaaaccgt
    10681 tattattctt atcagttata ggcggaggaa tgtcaggaag tttcattttc caattgatgg
    10741 atgctggact aagagcgcca gcttctccag ggtcgatcat tgcgattcta gcgatgacac
    10801 cgttcaattc ttaccttcct gttattttgg gcgtgattgt tgccgcagct gtttcgtttg
    10861 gtatttctgc agtaattttg aaagcggatg cgaaagatac aaatgaagac ttcgaaaaat
    10921 ctgttcaaga gacaaaggca gctaagcaag cttccaaagg tgcccaaggt attcctcaag
    10981 gagctacatt ctctggcgta caaaaaatca tttttgcttg tgatgcagga atgggatcaa
    11041 gtgcgatggg cgcttcgatc ttgagaaaaa aagtacagga agcagacttg cctcaaacgg
    11101 tgaccaatca agcaatcagc caactgacag atgactcgaa tacattgatc gttactcagg
    11161 tcgaactaca agaaagagca aaacaaaaag caccgagcgc gcgttttgtc tcagtagaaa
    11221 actttttgaa ttcacctcga tatgatgaaa tcattcaaga attaagcagc aagcagaaaa
    11281 catcagtttc tgaaattgag aatgagccag cagaagagtt tctttcagca aacatcaaac
    11341 aaatcaaaga gattctactt gtccacgatg atcgttccgg atcagcaaca atggggatga
    11401 ccgtgctcaa agatatatta gacaagaacc atttaactat cccgttaaga aaagtcaata
    11461 tccaagattt aaaagatgaa cctgattcac tgatcgtagc caaagaaagt ttagcggaaa
    11521 aagtacagat ggctgcacct caatccattc atttatcagt caacagcatg gtcaatccgc
    11581 aaaaatacga atcgattgca caatatttga aacagacagc ttaagggagg aaaacagcat
    11641 gcaaagtttg ccattcgaca tgatcgagct caatcgatct tttaaaacca aagaagaagc
    11701 gatccgctat tgcggtcaga tgcttgtaga agcagggtgc gttgatgagg aatatatcga
    11761 tgccatggta gatagagaca aaatgttatc tgtttatatg gggaatttta tcgcgattcc
    11821 tcatggaaca gatgaagcaa aaaaatatgt gaaaaaatcc ggcatctgtg tcgttcaagt
    11881 accagatggt gtagattttg gtaccgaaga agaagaaaag attgcgactg ttttgttcgg
    11941 tattgcagga gtcggagatg agcatttgca acttgtacag caaatcgcac tttattgcag
    12001 tgatatggac aatgttgtcc agctagcaga cgcgctcagc aaagaagaag taacggaaaa
    12061 tctagcaatt gcttaaggag agagaaaaat gagagcagta cattttggtg caggaaatat
    12121 cggtcgagga tttattggag aggttttggc agctaatgga tttgagatta ctttcgtaga
    12181 tgtgaatcac acgatcattg atgctttaaa tgaaagaaaa gaatacgaaa tcgaattagc
    12241 agatgaatca aaagaaaaaa tcactgtaaa aaatgtcaaa ggaatcaaca atcaaacaaa
    12301 tccagaaaaa gtaatccaag aaatcgctga agcagattta gttactaccg ccattggccc
    12361 gaatatctta ccttttattg ctgagttgat tgcaaaaggt atccaagaaa gagaaaagga
    12421 aggaagcaca acatctttag atatcattgc ctgtgaaaat atgattggcg gttcagcatt
    12481 tctagaaaaa gaagtgtata aatatcttcc ggaaacatca tttactgaca aatatatcgg
    12541 ctttccaaat gcagcagtcg atcgaatcgt cccattacaa caccatgaag atcctttatt
    12601 cgtccaagtg gaacctttca aagaatgggt cattgacgat agccaaagaa aaaataaaaa
    12661 aatccaatta aaaggggttt tatatgtaga tgatctagaa ccatatatcg aacgcaaact
    12721 atttagtgtc aatactggtc atgcgactgt cgcttataca ggtgccttgt taggttacca
    12781 gaccattgat gaagcaatgc aagatgcgtt agttgtggca caattaaaat cagttttgca
    12841 ggaaacagga agtttgctga ttgccaaatg ggggtttgat gcagagcagc atcatgcgta
    12901 tattgaaaaa atcattcatc gcttccaaaa caaaaatatc tctgatgcga tcacacgtgt
    12961 cgctcggaca ccattacgta agttaggtta tcaagaaaga tttacacgtc cagtgagaga
    13021 actacaagaa cataacttga cttgcccaca tttgacggca acaatgggga ttatttttaa
    13081 ttactatgat cctgaagatg aacaaagccg tcaattgcat gaaatgaaga tccatgaaaa
    13141 cttggaacaa ttgattcaag aagtcacagg aatcaatgat ccgaaaacaa tcgggaatat
    13201 caagcaaaac gtgaaccgtt atgccaagca agtcgcttaa aataggatac gaaaaagggg
    13261 aatgtgacga aatttttgtc acattcccct ttttcgaata actaggctgt ttgtccttca
    13321 agatagggca gatccaattc tttaccgtaa cgttgttcta aagctaggcg gatcaaacga
    13381 gctgccaggt tctcatttct tgcaaggata ggtccatgga aataagaacc gaagacattt
    13441 cggtaaatga caccttcaga ttggtcttca ccattatttc cttgaccttg taccactttc
    13501 cccaaaggtc gttcaccttc acctaagaaa gtccgaccat tgtggttttc aaatccataa
    13561 taagtttcat tgaattcttc gttatggata acgatatcac cgataaaccg gttattttcc
    13621 tgacttaacg tgtagtgatc cagtgcactg attccttgga tcttttctcc attagctccc
    13681 atgtagtagt gacctagcaa ttggtagcca ccacaaatgg ctaacatgac gccatcattt
    13741 tcgatatagt tgatcaaaga atctttcttt ttctggatgt cttttgaaat gattaattgc
    13801 tcaaagtctt gaccaccgcc aaagaaaacc aagtcatatt tatcagcatc aaaaggttca
    13861 tgtatactaa cgatctccgt atggcaagaa acccccattt tttctgctat atatttcaac
    13921 atcaaaaggt ttccgttatc accatatgtg ttcatcaaat ttccataaag gtgggcgata
    13981 ttgagttcat aattagccac ggttcattcc tcctttgata aatccttgag cagctagttc
    14041 tttacgaagc tgcaatacag cagtgtaagt agctaaaata tagacatgct cagttggcaa
    14101 ttctttgatt ttttcgatta cttctggtaa ttcttttgtt tctgtcaatt tattttctgg
    14161 aatcccggca acttttagac gtaaagtcat atctgtatga cggtctcctc cagaaataac
    14221 agctgggata tccatatcgg caaagttttc aaaattacca tcccaaatcc agctgacatc
    14281 gattccgtct gcgtaattgg catttaataa agccactaaa gaaaatggat aaggagctag
    14341 accaatcata tcgattactt gattgatacc tacaggattt ttgacaagta ctaatgtaca
    14401 ttttttccca ttgatattga tgacttcttg tcggccgaat actttctcgt catatcctaa
    14461 accttgtttg attttatcag gatcgacatt gaagtactct gccactgcag tagctgctaa
    14521 agcattgtag acattataca taccgccaac ttcgattccg tattcatgac catcgatcac
    14581 gaaatctgct gacacattgt ccatccgtac catatcagtt aatcgataat ccaattcagg
    14641 acgtttgaag tcacaatttg gacagtaata tttccctaag tttgcataag tgatcatttt
    14701 ataatgcaag atgtgatgac aatttggaca taagacaccg tctgtattgt aatgagccat
    14761 ttgttcacga tcttcttcat ggtcaaagcc ataatatttt ctaggattga ctgtttcgat
    14821 agaattgaag attggtgaat caccgttact gataatcgtt gcatttggtg actttgcggc
    14881 accgtctaca atcattttat aggtcgtata gatttctcca taccgatcca tttgatcacg
    14941 gaaaatattt gtaaataaga acagttctgg tttcatatat tctgtgactt tgctcaaact
    15001 tgcttcatct atttctagga cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa
    15061 gaaagtcgat acaattccct gtaccatatt cgcaccagta gggttggtta gaacttcatc
    15121 gaattgctgg cgcaaaatat tgactgttaa agcggtagtc aatgttttac cgtttgttcc
    15181 ggtgacaacg acgacttggt agtcttttgc caaactatct agtatatgtg gatcgacttt
    15241 cagcgctagt ttacctggta aacttgatcc gcctttaaaa aaagttttta atgcccattg
    15301 cgacgttttt ccaactgcaa tcgcaaaatg actgcgtatt cccattatgt aacctccaaa
    15361 aacgatttgc tgacgaataa attcaactaa tcataccata tgttgaaaaa aaggttaaat
    15421 tgcttttctt attcttaaga aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg
    15481 catgatttaa caaaaaaaca gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta
    15541 ttattccctc ttacctatat tcccatttta accgataata attttctcgc ttggatattc
    15601 atagcctaga tctcgtgttt cttttggcac gaataacatc aatgtatata gcattccgat
    15661 ccgcccaata aacatcagtg tagcaatcgt gattttgccc acaacagaca agtctccggt
    15721 aatccctaaa gataaaccag ttgttccaaa cgcagaagtt acttcgatga tgatcgagat
    15781 cagcgtttgt tcttctgtag cagataaaaa aacgatacaa aagaaacaca taccaagaga
    15841 aagcataaag acaacgactg attttcgcac atcatcttga tcgattctcc gtccaaaaat
    15901 attgatgtta tcttcacttt tgaggaagga atatagatac aaaccgatga ttgctacagt
    15961 agttgttcga atcccgccgc ctacagaact aggactgcat ccgataaaca tcagtaaaga
    16021 aaaaatgatc agtgttgtga tttgaaaatc acccaaatcg tgaatctgca gaccagcatt
    16081 acgagtcgta atcgaataaa acatcgaatt gatccatttg acactcatat tggaatcttg
    16141 gaataaatgg tccttttcca atagataaat caagacagtt cccgaaacaa acaagataac
    16201 aaaagctaac acagctaatt tggtaaacaa ggaaaaacga aacggaagct tcgcattgga
    16261 acgtttgtat aatagccatt cccggcactc catcaataca ggaaaaccaa ttcctccgac
    16321 aaaaatcaaa aacatgatca ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga
    16381 atccccagtg acgtcaaacc cagagttcgt gacagccgat atcgcctgat aaaaaccgta
    16441 aaaaacggca tcacgccaac gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa
    16501 tgtcccaaaa agtaattgaa accagattaa aatagcaaaa gtgatccgaa tcagtcggac
    16561 gataccagac aaacgcggtt gattcatatc cgtcatgatc agctgtcgct gtttcagtgt
    16621 gatccgtcgt ttagaaaaaa tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc
    16681 aacttgaaat aatatttcca gcagtataat accgttatca ttgaacacag aattgatatc
    16741 aaaagtcgat agtccagtca cactcactgt actgattgcc atgaacagca tgtccacaaa
    16801 agaaacatgg ctatctggtt cgcgaaaaat cggcatataa aatagaacga gtgataagac
    16861 cgtcatcaat atataataaa aaacgatgat ttggatcgat gagaaatgat tggcagcaaa
    16921 cttctgtcct cgccgctgca gccttcgaaa gaataaatca acatgcacag tactatcctc
    16981 ctgacaatag tcgtttctac cagtatacaa ggggaattag gaatactcaa agaaattatc
    17041 aaacagataa caatacagct ttcgattaaa atagaaatgg cttataataa ggaaaatcct
    17101 agaaaaaaga aggaggagga ataacaaatt gattaatgct caacaaatcg tagaaaaaat
    17161 gaaaaatcaa aaaataaatt atgatacagt tctgaaaaaa atgatcatgc aatgggagaa
    17221 agaagaagta aagcctaaga tattaataca tacatgctgt gctccttgca gtacgcacac
    17281 attggaattc atgtctcaat atgcagagat cacgttattt ttttcaaatt cgaatattca
    17341 tcctaaaagt gagtatttgc gaagattaca tgaacaaaaa cgatttgtag accaatttaa
    17401 tgagaaaact gaccatcatg ttgcgctaat cgttgatgaa taccgtccaa atgaattcac
    17461 caaaatggtt ttaacaaacc atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc
    17521 atgcttcaat atgcgattag atcttgttgc gcaaaaagcg caagaactag gatacgatta
    17581 ctttggaagt gccctgacca tctcgccaaa gaaaaatgca gctttgatca atcagatcgg
    17641 aatggatatc caaaagattt acgggacaca atacttgcca agtgatttca agaaaaacaa
    17701 aggctatgaa cgctctttgc aaatgtgcaa agagtatgat atttatcgcc aatgttattg
    17761 tggttgcgtg ttcgctgcca agcagcaagg atgcgatctg aaagaaatca atcgggaagc
    17821 aaaagcttac ttaaaaacat caaccgtagt ttttgaggaa aattaaagaa atctttcgga
    17881 aaaacgtaag aaaaaaataa agaatttatg gtagaaataa ggtagactgt ataatttgtc
    17941 cgtagtaaag taaggaaaca agaataaatt tcaatgaagg aagagataag atgcgaaaag
    18001 tagcacctat tttgaagcca atcgctgtat taacaatctc gacgattgtc gcgactacta
    18061 caggtattgt gaatagttcc agctaccgtg caccaagttc aagttctagt actgtagaag
    18121 aaacaacacc aactacttca gtctcgacat ctgaaccaac aacaacggaa agtttgaatg
    18181 aaaccactac gacaagtaca tgggacgata caacaactag ttcgtcgtat gatgagacat
    18241 atacaactga cagctcaagc gaaacaagta atacggtacc agatggcact ccatcggatt
    18301 caaccaatcc aagtacaagt ccggatacaa gtgctagctc cgaaacgata ccaagcagta
    18361 cgactgtaga tgataacacg caaaatgatg atgcagcacg tacagcacag caaaaccaag
    18421 attcaggaaa tacccaaacg tataaccagc aagcaggtga ttctcaatga catttttact
    18481 agaccttatc aacggggtgc ataaattcct tggctattta gacatcagtc ctaaatatct
    18541 taaccgtgga tatacgattt taagtgtgat tcccacgctt tatttattga gaattgttta
    18601 tggactttgg caaaaccaaa actatcttca atttttctta tatggacttg catttctagt
    18661 gcttgtttat tttactgttt taaatgtatt ttattacttt ttagataaaa acacgaaagc
    18721 cgatgtcact cagctttttg tgaagtattt acctgatgaa gcgttcaaca tccagacaga
    18781 agaatcgata gataatggaa attcgatcga taaagtgaat acacaagagg tcatggtttc
    18841 ttatgacgaa gattatcaat tgaaattagc cgagaatatg cggtatttga ttggaaatgg
    18901 tgagatcaaa acaaatgaac tcggtcgcat ggatggattt ctggtcgata gaaatacatt
    18961 gtatccttat tactatgtca agaaaacagg agagaaagag tatgttctaa atattggacg
    19021 aagctactcg gacatggaaa aagtagggac cattcgactt gactcaacag aagacacatt
    19081 ggaacctgtc ggattgttca tcgtcggcgg tgattttgtc aaagacgggt tacgctatca
    19141 tgagccttat cgtctgaaat tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt
    19201 ttatagtcgt agccaaagaa gaagacattc ataatgtgat gaaacgccgt gtatcagtgg
    19261 ttgcgataaa aacgtttatt cggaataagg gggatgtgac acgtcttttg tcacgttcct
    19321 tttttatata acgagaaaga taagacgaac acatcactta ttctttcctt tcatttataa
    19381 acattgtaga ataaagaaag tacaacgaag tattgtgaaa aaacgtaata aataacgagg
    19441 aaaatacaag taggaggtaa gctgtgaaag aatttttgaa aaattggggg attttgatcc
    19501 ttgtattagc ggctatttta ttagctagag tgtacgtgtt tacaccagtt actgtaaacg
    19561 ggcattcgat ggaccctaca ttaagtgatg gacaacgatt gatttcatct aagatctcaa
    19621 actatgaacg aatggatatt attactacaa aggaaccggg ggatgaagaa cggatgattg
    19681 ttaaacggat catcggtatg cctggagata cagtcaagat ggaaaatgac cagttgacca
    19741 ttaatggcaa aaagtatgat gaaccttatc tggatgagtt caaaaaagaa ttttcagaag
    19801 ataagttaca aggagaatat gcttatagct caggattcca agcacaagca gaaagttcat
    19861 ccacctttac gagtgacttt gaatatactg tgccaaaagg aaagtatctt gttctaggcg
    19921 ataaccgctt gatttcaaaa gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc
    19981 aaggaaaagt tgttttccgt tattggcctc tttcagaaat taaaatcatg tgatgaaaaa
    20041 ataaaaagaa gctgtgacat gttttttgtc acagcttctt tttccgaaaa ttccttctta
    20101 tttcttggag ttgctcactt tttttgcaat ctcattttaa gctttttggc tgatggataa
    20161 gaaaagcgaa gcgtttttgt tcataaatag cttttcccca cgatggaagg tatgactagt
    20221 agcagagaca aagcccgttt tttccaatcg attattcaat tcttcttggc taaagccatt
    20281 atggactttc ggatgactaa tttgttcatt cttgtcaaag tctacgatga tcaattgacc
    20341 gccaggagct aaaatttcat aaagttttgt aagaatattc tccgtatcag gaatgtgtaa
    20401 aagtacaagt gatagaagga tgagattagc ttggacagca ggaaattcga cagaagcatc
    20461 agcgtggatc gttcggacat ttttcagatc tgctgcttgg atcttttctt ctgccatttt
    20521 cagcatagtt tctgaagcat cggcaatgat cagttctttg aatctttccg caagcggtaa
    20581 actaacaagc ccagttccgc ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg
    20641 tagataagta gttagttctt cgcgaataat cttggctaat gcttgacggt caggggaatc
    20701 ataatggtta gcgatggaat caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa
    20761 ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga aagcatgcta tactcaattc
    20821 aaaaagggag aatctagaaa gaatatgccg atatcgtttg taaaagatag agaggaaaaa
    20881 ggaaagtgtg tccgagaaat attactagac ttgcctgagt ggtttgggct tcctgaatca
    20941 acggaaaaat atatcgagga atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa
    21001 gaatatttag gctttatcac tctttcacag acaagtgaag atactgccga aatctattct
    21061 attgtatggg aataaaaaaa gactatcatc accaaggatt aggaacaaaa ctttttaaag
    21121 aagcagaagg ttacgcagca aaacattaca aatatttgca agtgaagact gtagacgagg
    21181 ggcactactc tatatatgat caaactattt gtttctacga atcattagga ttttcaaggt
    21241 tggaagtttt tccaaattta tgggatgaat ggaacccatg tttagttctt gtcaaaaaat
    21301 tggaacaaaa gtgagcaata gatttaatac taatctgtgc ttgatgaaaa ttctcactgt
    21361 aaaaaatgct gaaaatcaag tataatacca taggaacaaa ctggaaggtg aaaaagagca
    21421 tggcacaact attttttaag tatggcgcaa tgaatagcgg caagacgatc gagatcctaa
    21481 aagtcgcaca taattatgaa gaacaaaata agccagttgt cttaatgaca agcggtattg
    21541 atacaagaga tggtttaggc gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc
    21601 cgatatttga agaaacagac gtatttgacg taattcagaa aatggaaagc aaaccctatt
    21661 gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca tgtcattgct tttacgagaa
    21721 ttgtggacga attggatatt ccggttatgg catttggttt gaaaaatgat tttcgaaacg
    21781 agttatttga aggatcaaaa tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa
    21841 caatctgttg gttctgccac aaaaaagcaa tcatgaattt acattatatt gatggaaacc
    21901 ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga agcttattac cccgtttgtc
    21961 gaaaacatta ctttcaccca tcaatgatta cagaaggaga ataagcatgt acgatcaatt
    22021 acaagcaatt gaagaccgtt atgaagagct tggagaactt ttaagtgacc cagaagtgat
    22081 ttctgataca aagcgtttta tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt
    22141 cgaagtttat cgtcgttata aagaagtagt acaaggaatc accgatacag aagaactgct
    22201 tggtgaaaaa ctggatgatg aaatggctga actagcaaaa gaagaattat ctgaattaaa
    22261 aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg cttttaccaa aagatccaaa
    22321 tgacgataaa aatatcatca tggaaatccg tggagcagca ggcggagacg aagcagcatt
    22381 attcgctggc gatcttttca atatgtacca aaagtatgct gaagcacaag gctggaaaac
    22441 agaagtgatg gaagctaacg ttaccggaat cggtggatat aaagaagtga tcatgatgat
    22501 caccggagac aacgtatatt cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg
    22561 tgttccttca actgaatcgc aagggcgtat ccatacttcc accgcaacag ttgtcgtgat
    22621 gccagaagct gaagaagtag agatcgatat tgaagacaaa gatatccgta cagatatcta
    22681 tcatgcttca ggtgctggcg gacagcacgt caataaaaca gcatctgctg tacgtttgac
    22741 tcacttacca acagggatcg ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg
    22801 tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca
    22861 aagcgagtat gatgccaatc gaaaatcagc agtcggaact ggggatcgtt cagaacggat
    22921 ccggacgtat aatttcccgc aaaatcgtgt gactgatcat cgtatcggat taactatcca
    22981 aaaattagat cagattttag cagggaaatt agatgagatc gttgatgcat tgatcatgta
    23041 tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa aatgacctat cgagaagtcc
    23101 tgtcacgggc ttcttctttt ttagaggatc atgggaaaga agcatatagt atccaatttt
    23161 tatttttgga acgaaaacaa tggaaaaaac ttgattggct ccttcatatg aacgaagaga
    23221 tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc
    23281 cacctcagta tttgctagga tatgcagatt tttatgatca tcggttaaag gtaactgaag
    23341 caacactgat ccctcgacca gaaacagagg agttagtcga atggtgttta gacgagacgc
    23401 ctggtgtacc gttagaagtg atagatattg ggacagggac tggagcaatt gctattagtt
    23461 tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga tctttcagaa gaagcattag
    23521 aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat cagtttttat catggagata
    23581 cattaggacc agtaatggat cagcaatttg atgtgattat ttctaatccg ccgtacatca
    23641 gtcggaatga atgggaactg atggatgaaa gtgtccggtc attcgaacct aaaatggccc
    23701 tttttgctga gaacgatgga ttagctattt atgaaaaaat agcagttgaa gcaccaagcg
    23761 tactaaaatc aaatggaaag atttttttag aaatcggatt tcgccaaggt gaagcagtaa
    23821 aaaaaatatt tcagcaagca tttccagata aaaaagtggc gataaaaaag gatctttttg
    23881 gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt
    23941 ttttttatta taaaaaaaca cttatttgac aaggatgtaa acgacttttt ggtttatcaa
    24001 caagtttatc cactattgac aaatagatta tccacaaaca gtggaaaact tgaggataaa
    24061 ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat aaactgtgga aacgaaacga
    24121 tatgatgaaa cagaattgca agaggcagcc gaagcactga aagaaggaga acttgttgct
    24181 tttcctaccg aaacagtata cggattgggc gccaacgctc ttttaccaaa cgctgtcaag
    24241 aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat taatcgtcca tgtcgcctct
    24301 tttgaacaag taaaagagta tgtggacaac tttcatccgg cagcagagaa gattgtgaag
    24361 aacttctggc ctggaccatt aactttgata tttaagatca aaaaagacac tttgccctca
    24421 gttgtgacgg gaggattatc cacagctgca tttcgtatac cggataacaa aaaaacacta
    24481 gaagtaatcg aactttccgg tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa
    24541 ccaagtccaa cgactgcaga tcatgtatat catgatctac aaggaaaaat taccgggatt
    24601 attgatgatg gagctacgcg aattggtgta gaatccactg ttcttgattt aagtgatcca
    24661 acagccatgc ctatgatttt acgaccaggt gcagtaacga aagaacaaat cgaagcagtc
    24721 atcgaatctc ctgttgctat tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc
    24781 cctgggatga aatacaaaca ctactcaccc gatacacgtg ttctgatggt cagagatggc
    24841 gattggtcaa ctgcagttca atgggcaaaa aataagaaga tccgtgcagg tgtcatagca
    24901 agccccgaaa ttgcggatca agtgcggaca gatactgcag ctgtttatat gtataacgac
    24961 aactcagtag aagcggcggc taaaggattg tttgcaggtc taagaggatt agatgaacca
    25021 acacttggat tagatttgat ttttgtacaa gtgtatccag aaactggatt ggggaatgct
    25081 tacatgaatc gcttgaaaaa agcagcagga caaaattatt ttgaaaaatg atcaattggt
    25141 tattgtcgca ttcttttagt atgatacaat gctatcaagt aaatcttcta tcagtgaacg
    25201 aaaaactaag cgtaactaat cgtaatagaa gagatacgaa tatttggcaa tttgtttttg
    25261 atgggaaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt agattacaaa
    25321 acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca agaacataat
    25381 ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc acaaggaagt
    25441 atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg tgggtgtgaa
    25501 tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa
    25561 tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta tctcgctttg
    25621 gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca tctaacacat
    25681 ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg tgttgatccg
    25741 acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca tcaaccaaaa
    25801 ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa attcagagaa
    25861 atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc aggattagta
    25921 gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac taccactcat
    25981 aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc attagcaaaa
    26041 aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca tgtgattgcg
    26101 ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata tagtgaacaa
    26161 atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg aaccagagtc
    26221 atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat
    26281 ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa aaactcgatt
    26341 ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac gccagcaatc
    26401 actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca
    26461 ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt acgcgaatta
    26521 actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta aacagaaaag
    26581 ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac aatatcggtg
    26641 atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag ggaggaaaag
    26701 tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc
    26761 ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta gaagaagtac
    26821 aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg gatactggcg
    26881 aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat aaattaccag
    26941 ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc catgataaat
    27001 tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat
    27061 caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg caacagctga
    27121 ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca ggagattcag
    27181 taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa
    27241 tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca gattcctatc
    27301 atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt tgggaccaat
    27361 atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa
    27421 atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat gtattaagag
    27481 atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac
    27541 gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag acaaaggcaa
    27601 cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa gctttgcttg
    27661 tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg agtttgacta
    27721 gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag gagagcttaa
    27781 aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact aactattatt
    27841 cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg
    27901 atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga aacaccgatc
    27961 actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc aatcttgcgc
    28021 gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa agtgggacat
    28081 atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt caaacttcca
    28141 gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac aggcggatca
    28201 gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt
    28261 ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga tattgacatc
    28321 tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc tggactaggg
    28381 gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa
    28441 cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc tttttcacag
    28501 ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt
    28561 ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt
    28621 ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc
    28681 agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc
    28741 aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc
    28801 tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt
    28861 ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc
    28921 ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata
    28981 gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc
    29041 tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata
    29101 aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact
    29161 gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc
    29221 accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta
    29281 ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac
    29341 ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata
    29401 ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc
    29461 catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa
    29521 aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct
    29581 tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta
    29641 ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat
    29701 atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat aactcctact
    29761 attcagtttt tgaagataga gtcagtatac atgaagagtt aaaataggca atgttttttt
    29821 atggatattt gtattgctaa aaatttatct tatatattca ttttgattat actactttta
    29881 tttgtaaaaa ttttctagca aaaccaagtg cttgcaactg attaagcgta caatttttta
    29941 gatgttccga gcgtacttca atcatttcaa aggagttgct acttaaatcg atatttttta
    30001 aatcagtatc aaaaaagcta gcattttcta aatcattagt agaaaacatt attttagtga
    30061 gggaacagtc tacaaaatct gcgcttgata agacagagtg ctcaaaggta ctttctttta
    30121 tgtttgaacc agataaattt acatagtcca ttttacaatt ctcgaataac gtgttcccaa
    30181 tataactttc catcaattta gtacctgtta atttacaatt gacaaatttc acgcgatgca
    30241 acatgctttt tgtcaatata cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa
    30301 tctccattct ttgaaaatca ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa
    30361 taattggatg gcagattatt tggttttcga tgacctcttt ttgtgaaagg atattataca
    30421 gttcaaaatc ttcgttttca tagatatgaa aaaaggatcc tttattcaag cttgcttcat
    30481 taactttagg aaaaatcata tttaattacc tccattttca taaagtcata ttaacaaaag
    30541 ctaaaccaac ttacattagc agaaaatgag ataggttttc gcctttttcg ataacgatag
    30601 tgtcgctatc tcttgaaacg ttcttagaat tgccctgaga gacacaaatt aggcgtaatt
    30661 ctaataaaaa tcttagaata aaaattttta ctgatttaat ttagaatgtt tttaaaataa
    30721 aaatcgttgc cataacagtg tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa
    30781 tctagatgta gcgtatactt tgtttataga atgtagtggg aagaaatata tttttgggag
    30841 gaaatcgtga tgtctaaaag tgaagaaata gaaaacatgg tactaaaatt atatgatctc
    30901 gttttaaatc cagagatcaa agaaaaggaa cgtatacttt tgatagacgc aaagacaggc
    30961 ttagaaaaag gtcagtatta tcctaaagtc ataaataatt tggaacgtag tttacgtccg
    31021 ttagcaatca ggggagaatt gtctaaacca gtatcaccgt tttatatgga gatatcaaca
    31081 ataggaaaat ttgaaaaaga attaggtcga ggaatggctt cggcaccgat tacttttggg
    31141 catttataaa aacgagttat tcatctcaat gaattaaaaa gtatagtcaa tctcataatg
    31201 caggctaggt tcatttattt gaacagagtc tgtatttttt tattctaaat aaagaaacag
    31261 atgtttcttt atattgtatt ttatccttta attaacacaa aaacagctag tataaactaa
    31321 taatgttaat taaaagtgta ttttatgaaa ctatcgttat ttattaatgt tgcatttttg
    31381 taagttaaaa attggaaata tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa
    31441 tatatgcgaa aaaataattt acattaggaa aaaccttgta aaaacagttt tttggcatat
    31501 ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag attggaaatg
    31561 agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt ttatttttag
    31621 ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat
    31681 ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag
    31741 atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa
    31801 acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc
    31861 tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc
    31921 cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg
    31981 ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga
    32041 tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga
    32101 gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg
    32161 ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt
    32221 tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc
    32281 aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac
    32341 agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta
    32401 catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca
    32461 atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt
    32521 ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa
    32581 gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag
    32641 gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt
    32701 tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt
    32761 cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag
    32821 aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct
    32881 ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta
    32941 cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt
    33001 ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac
    33061 gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg
    33121 gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt
    33181 caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt
    33241 cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga
    33301 cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa
    33361 aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca
    33421 tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca
    33481 tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac
    33541 cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa
    33601 aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta
    33661 agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta
    33721 gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc tgtgctggga
    33781 tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt taaatatgac
    33841 tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt ttatatatgc
    33901 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
    33961 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
    34021 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
    34081 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
    34141 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
    34201 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
    34261 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
    34321 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
    34381 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
    34441 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
    34501 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
    34561 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
    34621 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
    34681 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
    34741 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
    34801 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
    34861 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
    34921 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
    34981 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
    35041 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
    35101 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
    35161 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
    35221 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
    35281 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
    35341 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
    35401 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
    35461 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
    35521 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
    35581 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
    35641 caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
    35701 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
    35761 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
    35821 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
    35881 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
    35941 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
    36001 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
    36061 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
    36121 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
    36181 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
    36241 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
    36301 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
    36361 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
    36421 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
    36481 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
    36541 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
    36601 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
    36661 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
    36721 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
    36781 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
    36841 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
    36901 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
    36961 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
    37021 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
    37081 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
    37141 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
    37201 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
    37261 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
    37321 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
    37381 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
    37441 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
    37501 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
    37561 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
    37621 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
    37681 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
    37741 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
    37801 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
    37861 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
    37921 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
    37981 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
    38041 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
    38101 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
    38161 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
    38221 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
    38281 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
    38341 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
    38401 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
    38461 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
    38521 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
    38581 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
    38641 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
    38701 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
    38761 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
    38821 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
    38881 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
    38941 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
    39001 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
    39061 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
    39121 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
    39181 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
    39241 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
    39301 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
    39361 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
    39421 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
    39481 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
    39541 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
    39601 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
    39661 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
    39721 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
    39781 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
    39841 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
    39901 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
    39961 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
    40021 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
    40081 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
    40141 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
    40201 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
    40261 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
    40321 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
    40381 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
    40441 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
    40501 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
    40561 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
    40621 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
    40681 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
    40741 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
    40801 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
    40861 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
    40921 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
    40981 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
    41041 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
    41101 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
    41161 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
    41221 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
    41281 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
    41341 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
    41401 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
    41461 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
    41521 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
    41581 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
    41641 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
    41701 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
    41761 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
    41821 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
    41881 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
    41941 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
    42001 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
    42061 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
    42121 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
    42181 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
    42241 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
    42301 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
    42361 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
    42421 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
    42481 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
    42541 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
    42601 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
    42661 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
    42721 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
    42781 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
    42841 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
    42901 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
    42961 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
    43021 tgacagcctg cttgatgggg gttttagcaa tt
//
LOCUS       DACETN010000012        42353 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DACETN010000012 DACETN010000000
VERSION     DACETN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.013"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(299..853)
                     /locus_tag="IXF98_000615"
     CDS             complement(299..853)
                     /locus_tag="IXF98_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1554950.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(979..2909)
                     /locus_tag="IXF98_000616"
                     /pseudo
     CDS             complement(979..2909)
                     /locus_tag="IXF98_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3215..3931)
                     /locus_tag="IXF98_000617"
     CDS             complement(3215..3931)
                     /locus_tag="IXF98_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1554951.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3932..5755)
                     /gene="glpO"
                     /locus_tag="IXF98_000618"
     CDS             complement(3932..5755)
                     /gene="glpO"
                     /locus_tag="IXF98_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1554952.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5769..7265)
                     /gene="glpK"
                     /locus_tag="IXF98_000619"
     CDS             complement(5769..7265)
                     /gene="glpK"
                     /locus_tag="IXF98_000619"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1554953.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7401..8861)
                     /locus_tag="IXF98_000620"
     CDS             complement(7401..8861)
                     /locus_tag="IXF98_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1554954.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8851..10146)
                     /locus_tag="IXF98_000621"
     CDS             complement(8851..10146)
                     /locus_tag="IXF98_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1554955.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10478..11155)
                     /locus_tag="IXF98_000622"
     CDS             complement(10478..11155)
                     /locus_tag="IXF98_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1554956.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11185..12276)
                     /locus_tag="IXF98_000623"
     CDS             complement(11185..12276)
                     /locus_tag="IXF98_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1554957.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12377..12811)
                     /locus_tag="IXF98_000624"
     CDS             complement(12377..12811)
                     /locus_tag="IXF98_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1554958.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13395..14852)
                     /gene="ascB"
                     /locus_tag="IXF98_000625"
     CDS             complement(13395..14852)
                     /gene="ascB"
                     /locus_tag="IXF98_000625"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1554959.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14849..16744)
                     /locus_tag="IXF98_000626"
     CDS             complement(14849..16744)
                     /locus_tag="IXF98_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554960.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16802..18577)
                     /locus_tag="IXF98_000627"
     CDS             complement(16802..18577)
                     /locus_tag="IXF98_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1554961.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18627..20123)
                     /locus_tag="IXF98_000628"
     CDS             complement(18627..20123)
                     /locus_tag="IXF98_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1554962.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20317..21939)
                     /locus_tag="IXF98_000629"
     CDS             complement(20317..21939)
                     /locus_tag="IXF98_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1554963.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22267..23580)
                     /locus_tag="IXF98_000630"
     CDS             complement(22267..23580)
                     /locus_tag="IXF98_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1554964.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23825..24049)
                     /locus_tag="IXF98_000631"
     CDS             complement(23825..24049)
                     /locus_tag="IXF98_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554965.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24069..24437)
                     /locus_tag="IXF98_000632"
     CDS             complement(24069..24437)
                     /locus_tag="IXF98_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1554966.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24633..25343)
                     /locus_tag="IXF98_000633"
     CDS             complement(24633..25343)
                     /locus_tag="IXF98_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554967.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25361..25858)
                     /locus_tag="IXF98_000634"
     CDS             complement(25361..25858)
                     /locus_tag="IXF98_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1554968.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25895..26347)
                     /locus_tag="IXF98_000635"
     CDS             complement(25895..26347)
                     /locus_tag="IXF98_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1554969.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26482..27087)
                     /locus_tag="IXF98_000636"
     CDS             complement(26482..27087)
                     /locus_tag="IXF98_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1554970.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETSEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27537..28157)
                     /locus_tag="IXF98_000637"
     CDS             complement(27537..28157)
                     /locus_tag="IXF98_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1554971.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28159..29028)
                     /locus_tag="IXF98_000638"
     CDS             complement(28159..29028)
                     /locus_tag="IXF98_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1554972.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29051..29458)
                     /locus_tag="IXF98_000639"
     CDS             complement(29051..29458)
                     /locus_tag="IXF98_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1554973.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29477..30766)
                     /locus_tag="IXF98_000640"
     CDS             complement(29477..30766)
                     /locus_tag="IXF98_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1554974.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(30793..31095)
                     /locus_tag="IXF98_000641"
     CDS             complement(30793..31095)
                     /locus_tag="IXF98_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1554975.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31120..33213)
                     /locus_tag="IXF98_000642"
     CDS             complement(31120..33213)
                     /locus_tag="IXF98_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1554976.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33702..34430)
                     /locus_tag="IXF98_000643"
     CDS             complement(33702..34430)
                     /locus_tag="IXF98_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1554977.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34576..37197)
                     /locus_tag="IXF98_000644"
                     /pseudo
     CDS             complement(34576..37197)
                     /locus_tag="IXF98_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(37311..38642)
                     /locus_tag="IXF98_000645"
     CDS             complement(37311..38642)
                     /locus_tag="IXF98_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1554978.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(38608..40086)
                     /locus_tag="IXF98_000646"
     CDS             complement(38608..40086)
                     /locus_tag="IXF98_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1554979.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(40391..42226)
                     /gene="tdc"
                     /locus_tag="IXF98_000647"
     CDS             complement(40391..42226)
                     /gene="tdc"
                     /locus_tag="IXF98_000647"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1554980.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        12107 a         9188 c         7037 g        14021 t
ORIGIN      
        1 agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag
       61 agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt
      121 cagctgattg aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca
      181 agaaataatt tcaaaaacaa cttctcctgt ttttggtttg gttggcttat tatcgagaag
      241 tagctactgg gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt
      301 attttctttg aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac
      361 cgtaagtgat gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca
      421 gcaaaaggat catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc
      481 cacttaattt gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt
      541 ttatcaagaa tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa
      601 tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta
      661 cctgattata tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa
      721 tgaccgctcc tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca
      781 aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg
      841 tgcttaattt cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta
      901 cacggggaat aaaaccaatg atttcactgt ttttattttt ctttacagcc actgatatta
      961 tgagctaatt tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg
     1021 tttgcgcatt tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg
     1081 ctgccgttta cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta
     1141 cagatcttct gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt
     1201 gtctaaatat acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg
     1261 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
     1321 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
     1381 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
     1441 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
     1501 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
     1561 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
     1621 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
     1681 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
     1741 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
     1801 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
     1861 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
     1921 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
     1981 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
     2041 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
     2101 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
     2161 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
     2221 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
     2281 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
     2341 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
     2401 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
     2461 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
     2521 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
     2581 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
     2641 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
     2701 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
     2761 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
     2821 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
     2881 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
     2941 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
     3001 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
     3061 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
     3121 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
     3181 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
     3241 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
     3301 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
     3361 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
     3421 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
     3481 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
     3541 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
     3601 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
     3661 taatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
     3721 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
     3781 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
     3841 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
     3901 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
     3961 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
     4021 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
     4081 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
     4141 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
     4201 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
     4261 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
     4321 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
     4381 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
     4441 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
     4501 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
     4561 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
     4621 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
     4681 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
     4741 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
     4801 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
     4861 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
     4921 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
     4981 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
     5041 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
     5101 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
     5161 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
     5221 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
     5281 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
     5341 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
     5401 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
     5461 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
     5521 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
     5581 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
     5641 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
     5701 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
     5761 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
     5821 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
     5881 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
     5941 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
     6001 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
     6061 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
     6121 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
     6181 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
     6241 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
     6301 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
     6361 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
     6421 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
     6481 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
     6541 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
     6601 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
     6661 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
     6721 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
     6781 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
     6841 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
     6901 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
     6961 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
     7021 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
     7081 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
     7141 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
     7201 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
     7261 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
     7321 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
     7381 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
     7441 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
     7501 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
     7561 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
     7621 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
     7681 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
     7741 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
     7801 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
     7861 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
     7921 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
     7981 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
     8041 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
     8101 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
     8161 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
     8221 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
     8281 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
     8341 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
     8401 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
     8461 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
     8521 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
     8581 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
     8641 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
     8701 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
     8761 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
     8821 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
     8881 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
     8941 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
     9001 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
     9061 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
     9121 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
     9181 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
     9241 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
     9301 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
     9361 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
     9421 ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc
     9481 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
     9541 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
     9601 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
     9661 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
     9721 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
     9781 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
     9841 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
     9901 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
     9961 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    10021 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    10081 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    10141 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    10201 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    10261 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    10321 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    10381 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    10441 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    10501 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    10561 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    10621 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    10681 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    10741 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    10801 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    10861 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    10921 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    10981 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    11041 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    11101 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    11161 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    11221 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    11281 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    11341 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    11401 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    11461 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    11521 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    11581 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    11641 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    11701 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    11761 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    11821 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    11881 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    11941 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    12001 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    12061 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    12121 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    12181 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    12241 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    12301 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    12361 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    12421 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    12481 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    12541 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    12601 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    12661 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    12721 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    12781 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    12841 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    12901 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    12961 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    13021 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    13081 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    13141 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    13201 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    13261 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    13321 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    13381 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    13441 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    13501 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    13561 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    13621 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    13681 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    13741 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    13801 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    13861 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    13921 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    13981 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    14041 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    14101 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    14161 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    14221 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    14281 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    14341 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    14401 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    14461 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    14521 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    14581 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    14641 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    14701 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    14761 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    14821 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    14881 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    14941 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    15001 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    15061 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    15121 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt
    15181 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    15241 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    15301 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    15361 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    15421 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    15481 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    15541 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    15601 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    15661 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    15721 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    15781 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    15841 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa
    15901 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    15961 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    16021 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    16081 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    16141 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    16201 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    16261 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    16321 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    16381 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    16441 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    16501 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    16561 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    16621 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    16681 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    16741 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    16801 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    16861 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    16921 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    16981 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    17041 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    17101 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    17161 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    17221 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    17281 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    17341 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    17401 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    17461 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    17521 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    17581 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    17641 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    17701 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    17761 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    17821 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    17881 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    17941 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    18001 ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    18061 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    18121 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    18181 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    18241 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    18301 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    18361 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    18421 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    18481 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    18541 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    18601 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    18661 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    18721 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    18781 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    18841 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    18901 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    18961 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    19021 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    19081 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    19141 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
    19201 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
    19261 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    19321 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    19381 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    19441 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
    19501 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    19561 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    19621 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    19681 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    19741 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    19801 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    19861 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    19921 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    19981 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    20041 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    20101 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    20161 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    20221 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    20281 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    20341 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    20401 aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca
    20461 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    20521 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    20581 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    20641 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    20701 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc
    20761 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    20821 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    20881 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    20941 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    21001 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    21061 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    21121 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    21181 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    21241 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    21301 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
    21361 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    21421 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    21481 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    21541 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    21601 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    21661 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    21721 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    21781 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    21841 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    21901 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    21961 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    22021 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    22081 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
    22141 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
    22201 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
    22261 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
    22321 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
    22381 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
    22441 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
    22501 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
    22561 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
    22621 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
    22681 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
    22741 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
    22801 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
    22861 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
    22921 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat
    22981 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
    23041 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
    23101 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
    23161 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
    23221 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
    23281 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
    23341 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
    23401 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
    23461 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
    23521 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
    23581 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
    23641 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
    23701 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
    23761 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
    23821 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
    23881 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
    23941 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
    24001 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
    24061 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
    24121 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
    24181 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
    24241 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
    24301 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
    24361 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
    24421 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
    24481 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
    24541 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg
    24601 caatgaatgc cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg
    24661 gttagtgtgt gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct
    24721 tcgattgcct gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc
    24781 gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt cacgccagtt gagacagcga
    24841 cagatcgctc cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa
    24901 tctgcaccta aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct
    24961 gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta
    25021 tagatatcta caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt
    25081 aaagcttcaa tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc
    25141 cactgagcaa tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct
    25201 tttgctgcct cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta
    25261 tcgataccat ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg
    25321 gtatccacta ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga
    25381 atagatagtc tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa
    25441 atcctttttt acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa
    25501 tggctgtttt ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga
    25561 taccttgatg tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag
    25621 ccatttcctc agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat
    25681 cgatactcac taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta
    25741 gtgcataagc ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg
    25801 tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt
    25861 ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt
    25921 tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt
    25981 gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc
    26041 gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt
    26101 tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc
    26161 gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag
    26221 gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga
    26281 attcgatatt gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt
    26341 cagccatttc atcccacctt tattttgttt gtaagactag ttcacctctt tttgttgcaa
    26401 atgcaactat tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct
    26461 tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag
    26521 taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa
    26581 tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca
    26641 agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc
    26701 aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt
    26761 acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc
    26821 tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac
    26881 atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgga
    26941 agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg
    27001 ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct
    27061 tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact
    27121 atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa
    27181 taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg
    27241 aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg
    27301 acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct
    27361 ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac
    27421 atgagaagct gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg
    27481 acctctcggt aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac
    27541 ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta
    27601 tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg
    27661 gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc
    27721 aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc tgattcacga
    27781 tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc
    27841 acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa
    27901 ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa
    27961 tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg
    28021 atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat
    28081 cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga
    28141 atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa
    28201 cagtccaatc ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg
    28261 gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt
    28321 gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata
    28381 atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc
    28441 gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt
    28501 accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc
    28561 cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat
    28621 tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt
    28681 tgtcttcagg atattttctt tgaaaggcag atgggacccg tcatacatga cagaagtata
    28741 tccgctgtcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac
    28801 tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc
    28861 gatatattta gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc
    28921 tgcgcctagc atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa
    28981 attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca
    29041 ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat
    29101 ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac
    29161 gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt
    29221 ccaaactctt ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa
    29281 gtccaacttg ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt
    29341 gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt
    29401 cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca
    29461 ttttccctac tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga
    29521 gtgctgcggc tactgtatct acatcggttg ctgtagcatt gttgaatccc atcgaattaa
    29581 agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa
    29641 tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa
    29701 agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca
    29761 tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat
    29821 aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta
    29881 tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat
    29941 acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc
    30001 ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga
    30061 ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact
    30121 tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg
    30181 cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga
    30241 ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa
    30301 aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg
    30361 tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga
    30421 aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac
    30481 ctaaacccaa atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt
    30541 gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc
    30601 tcgaaccggc actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt
    30661 gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg
    30721 atgcctgagt caaaatattt tcgatgataa aattgataac gctcatgttt ttaccctctt
    30781 ttctttttgt atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat
    30841 ttcatttttt gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa
    30901 cttttcgaac tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac
    30961 tgcggaagaa atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc
    31021 ctcagcagac atctgtgctg caaaactact tcctaaacct gcgccgcata cgaataaaat
    31081 gttcaatttg ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa
    31141 aagcagttgt ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc
    31201 gttgttgatc aattcaatta gttctttcat gatgttcaga tgggagtagg agtctactgc
    31261 tgccaaacaa aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg
    31321 ctcaatcgta gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc
    31381 tagtgccatg tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat
    31441 cgcttcgaca tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc
    31501 aatcgattcc tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat
    31561 atcttttaac ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa
    31621 tttttctaac ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc
    31681 tattatttga gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc
    31741 ttcatttctt ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc
    31801 gacaactaaa agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac
    31861 atccaattta tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg
    31921 aggaaacttt tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca
    31981 gacaacgact gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga
    32041 aaagtggatg acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc
    32101 gatcgtagga acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt
    32161 ttctttcaat ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat
    32221 gtgcttatag agattttcga ataaggcttc ttcctttaga tggaaaggta cgtgtgtctc
    32281 gttctctaca aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga
    32341 attggagata tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac
    32401 ttgcgggaca ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct
    32461 actgtctttt atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat
    32521 tcgctggatc cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg
    32581 tttcaacaac ttattggaat agatctcctg tatttttttg atttcctcca aaggaaaata
    32641 atggagaaag attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt
    32701 cattcccaga ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc
    32761 tttataaacc agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt
    32821 tgctcgtaac cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc
    32881 ttttttgttt agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat
    32941 acgttcgcgc cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc
    33001 ttgcttttcc ttttccagct tcaacaaaaa acctttgttg cgaattgtac ggatctccgg
    33061 aagcttttta ctgctcaacc agatattcgc ttcaagaact tcctgacgaa tcgtccgctg
    33121 actgacattg aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat
    33181 tctgtgaagt atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt
    33241 atatggttcc gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag
    33301 taagcaaagc aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat
    33361 acgcctatta ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt
    33421 cctgcatcaa gtaataagaa caactaaact aaaatagtgc ttcctatttt ttgtttggtt
    33481 gttcttatta tcgcagatgc aagcttttaa acaccattta gtggagaagc tgtgacagag
    33541 atgttcagcc ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa
    33601 tcgtgcctta ttgccgatag gctatctctg gaacaccgtt tattcagaaa aaggagctgc
    33661 gacaaggctt ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggt
    33721 tgaataaaat cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga
    33781 gaaaaatact gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact
    33841 gaataatcac agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga
    33901 taatttccta cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg
    33961 tttttttgca gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct
    34021 ttttcaggag tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc
    34081 aaaaactgtg ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgatttc
    34141 agccttttct tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac
    34201 aaaacagcta atgcaagaaa ccccgtttgc tcataactag aaggacctgt acttggacca
    34261 taatacccat aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa
    34321 cgaccgccaa atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca
    34381 cccgcgacag cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg
    34441 attgctaaat aattgatttt ttcattgtct tcctcgttat cttttattac aaaaaactat
    34501 ttttatcata gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta
    34561 gcaattttgt cttatttagt cattgagtaa catcggacag atgtatgtgt cggcagtcca
    34621 taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg
    34681 agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc
    34741 actttgacta gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg
    34801 atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt
    34861 ctgtacatat gaatcattat ctagctcctt agctgcttgg attgcaagcc tgatatgcgg
    34921 ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg
    34981 accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac
    35041 atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac
    35101 actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc
    35161 aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg
    35221 atcaatagca taatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt
    35281 agcagactta tccaaaatgc gccgaccttc ttttacggct tttgcgatcg tatggaaatt
    35341 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
    35401 agcgattcct atatcggctc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
    35461 tccgacgatt tctccatttt tctgcaaagc tttcacgatt cgcagtttgt gttctggtgt
    35521 cgttcgagca aagacgtcga cttttggtac ttgtcgaatc aactcttctt cagacataag
    35581 atccaaatcg atcccttcta aaactttagc agtatgtttt aagccaattt gttccgcgat
    35641 cgcttttgct gtatctttgt gatcccccgt aatcattttc actgagattc ctgcttgttg
    35701 gcattccttt acagctttga ctgcactttc ttttggcgga tcaatcaacc cagcaatccc
    35761 tgcaaaagtt aattgggtta aagactcatg cgtgagttcc tgtttcgaat ctactttttt
    35821 gtaagcaaat cctaaaacac gctgaccttt tttcgcaaac gctgtggctg tttgacccca
    35881 tgcttcttat tctgaagaag aaagagttga tttagccaat aatacttctg gcgcaccttt
    35941 gacgtaaatc acccctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
    36001 gctgaacgga atttttgctt ctattttttt caaaggcaga tcggctgttt cggtgtggcg
    36061 aatcagtgca agttctgtcg gattgccact taaattttct gttttctgtt cttctttcgt
    36121 ctttaattcc tgacaatttt tcataataaa cagtacgcga gtttcttcct ttgctgctac
    36181 atccacaact gtcatttcat tttttgtcaa tgtccctgtc ttatctgaac agatcactgt
    36241 catagaacct aatgtttcta cagaaggcag tcctttgatg atcgcctttt cttttgccat
    36301 ttcatgtact ccgacagata ggatcatggt caacacagct ggcaatcctt cgggtaccat
    36361 tgacaccacc aaagcgatca tagctgaaag aaggatatgc caatccatgc catagcggaa
    36421 tgttgtaaaa acaactaaga acaagatcaa acaaagaatg ccttgaaaaa tttgtttgtt
    36481 caatcggttg atcttcttga tcagaggcgt cgtttgttgt tcgaccgatt gcagtgcttg
    36541 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
    36601 agattgtaca agcgttcctg aaaaagccat atttttttgg tcaccgattg attcttctgc
    36661 tgccaaaacg gaatgatttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc
    36721 tactagtaga tcatgtactt cgatcaaccg aacatctgct ggaacgacgt ctccggcacg
    36781 taagacaatg acatctcctt ttactagttc tttcgcaggg attttttctt gcatcccgtt
    36841 cgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaacg attcttctgc
    36901 ttttctttct tgccaataac taacgagtcc gttgacgatc actacgaata aaataatact
    36961 tccttcgatg tattcgctcg aagcaaattt caaaaaagag gcaatgatca acacgatcat
    37021 cagcagatca gtaaagtgtt ttgctatttt tttccatcgt tttgtctgct gtcttgcttc
    37081 tatctcatta tatccttgat cagtcaggcg tttctgccgt tcttcttgcg acaagccttc
    37141 tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct
    37201 atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg
    37261 attatttcag gggggtctga aataattcgt tttgcagaat gtatctgggc ctaactgata
    37321 tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa
    37381 aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac
    37441 accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat
    37501 tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct
    37561 gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccgaca
    37621 aaaaataaca tccctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca
    37681 caaaaccaat ggggcaaact tttctctttt gctgtttgat ataaaatcct gctagtactg
    37741 tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct
    37801 gctccgcttt ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct
    37861 aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagagg
    37921 atacgccaag gcagacagtt gattgcattt ttcaaattct tttgtgggtc actcgtctca
    37981 ccaactgtta atccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgaa
    38041 gcaatcattc cagcaaagcc aaacgggact aattcattga gctgtccaaa atttgctttg
    38101 attccaatag actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa
    38161 aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg
    38221 actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct
    38281 ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca
    38341 gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa
    38401 taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc
    38461 atgacccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct
    38521 ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca
    38581 cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc
    38641 atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt
    38701 ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc
    38761 aattagattg atccatgtcc cagctttcat cgtcagtccg acagaaaatc cgattgccgc
    38821 aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat
    38881 tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat
    38941 gatgacatag acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg
    39001 attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt
    39061 caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg
    39121 atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga
    39181 taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg
    39241 caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg
    39301 tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa
    39361 aattaacgcg gcaaatactc cgcgaatata cgttttaact ggatttttca accgagtgat
    39421 atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa
    39481 tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc
    39541 ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa
    39601 aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt
    39661 caaattcaaa aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct
    39721 gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcaa gtgctgaagc
    39781 gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca
    39841 tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc
    39901 ggcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat
    39961 gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac
    40021 aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc
    40081 tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt
    40141 caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat
    40201 ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga
    40261 acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat
    40321 tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca
    40381 aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttctt
    40441 ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta
    40501 gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa
    40561 aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc
    40621 gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt
    40681 cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa
    40741 aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat
    40801 gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat
    40861 atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc
    40921 cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt
    40981 caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga
    41041 ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg
    41101 cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga
    41161 aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt
    41221 cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat
    41281 agatcccttc atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt
    41341 gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgttcag
    41401 ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg
    41461 ggatagcaac catctgatct aagcctactc cgcaaatatc taacgccttc atccaagaat
    41521 agtgttttgt ttgaggcacc agccatttcc ctaatctttg gatatttttt ccgctacggg
    41581 aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa
    41641 tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact tgttcaggca
    41701 gcacttcctt gacagctaaa ggaatcgatt tgatgcagcg ggcgtaccat aatccttcaa
    41761 gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg
    41821 aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg
    41881 caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg
    41941 tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc
    42001 gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat
    42061 ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc
    42121 tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt
    42181 cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt cttccctcca
    42241 tattttgttg gtcaatgtct cgcagataca tgtgtttatc tgtaacacct cttcactata
    42301 gcgccaaaaa tgagaaattt ttttttataa catcaaatcc acgtagattt tta
//
LOCUS       DACETN010000013        38274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DACETN010000013 DACETN010000000
VERSION     DACETN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.014"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(62..973)
                     /locus_tag="IXF98_000648"
     CDS             complement(62..973)
                     /locus_tag="IXF98_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1554981.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1430..1858)
                     /locus_tag="IXF98_000649"
     CDS             complement(1430..1858)
                     /locus_tag="IXF98_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554982.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1886..2524)
                     /locus_tag="IXF98_000650"
     CDS             complement(1886..2524)
                     /locus_tag="IXF98_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1554983.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2528..4564)
                     /locus_tag="IXF98_000651"
     CDS             complement(2528..4564)
                     /locus_tag="IXF98_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1554984.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4690..5355
                     /locus_tag="IXF98_000652"
     CDS             4690..5355
                     /locus_tag="IXF98_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1554985.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(7290..10439)
                     /locus_tag="IXF98_000653"
     CDS             complement(7290..10439)
                     /locus_tag="IXF98_000653"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1554986.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(10452..11651)
                     /locus_tag="IXF98_000654"
     CDS             complement(10452..11651)
                     /locus_tag="IXF98_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1554987.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(11641..13236)
                     /locus_tag="IXF98_000655"
     CDS             complement(11641..13236)
                     /locus_tag="IXF98_000655"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1554988.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(13820..14527)
                     /locus_tag="IXF98_000656"
     CDS             complement(13820..14527)
                     /locus_tag="IXF98_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554989.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(14592..15428)
                     /locus_tag="IXF98_000657"
     CDS             complement(14592..15428)
                     /locus_tag="IXF98_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1554990.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(15446..16855)
                     /locus_tag="IXF98_000658"
     CDS             complement(15446..16855)
                     /locus_tag="IXF98_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1554991.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(16869..18692)
                     /locus_tag="IXF98_000659"
     CDS             complement(16869..18692)
                     /locus_tag="IXF98_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1554992.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(19535..19861)
                     /locus_tag="IXF98_000660"
     CDS             complement(19535..19861)
                     /locus_tag="IXF98_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554993.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(19907..20269)
                     /locus_tag="IXF98_000661"
     CDS             complement(19907..20269)
                     /locus_tag="IXF98_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1554994.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(20306..20521)
                     /locus_tag="IXF98_000662"
     CDS             complement(20306..20521)
                     /locus_tag="IXF98_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1554995.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            20648..20983
                     /locus_tag="IXF98_000663"
     CDS             20648..20983
                     /locus_tag="IXF98_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1554996.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(21059..23056)
                     /locus_tag="IXF98_000664"
     CDS             complement(21059..23056)
                     /locus_tag="IXF98_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1554997.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(23154..23429)
                     /locus_tag="IXF98_000665"
     CDS             complement(23154..23429)
                     /locus_tag="IXF98_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1554998.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            23661..24395
                     /locus_tag="IXF98_000666"
     CDS             23661..24395
                     /locus_tag="IXF98_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1554999.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            24434..25576
                     /locus_tag="IXF98_000667"
     CDS             24434..25576
                     /locus_tag="IXF98_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1555000.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(25671..27062)
                     /gene="sufB"
                     /locus_tag="IXF98_000668"
     CDS             complement(25671..27062)
                     /gene="sufB"
                     /locus_tag="IXF98_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1555001.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(27067..27537)
                     /locus_tag="IXF98_000669"
     CDS             complement(27067..27537)
                     /locus_tag="IXF98_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1555002.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(27524..28759)
                     /locus_tag="IXF98_000670"
     CDS             complement(27524..28759)
                     /locus_tag="IXF98_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1555003.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(28756..30042)
                     /gene="sufD"
                     /locus_tag="IXF98_000671"
     CDS             complement(28756..30042)
                     /gene="sufD"
                     /locus_tag="IXF98_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1555004.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(30055..30828)
                     /gene="sufC"
                     /locus_tag="IXF98_000672"
     CDS             complement(30055..30828)
                     /gene="sufC"
                     /locus_tag="IXF98_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1555005.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(31025..31873)
                     /locus_tag="IXF98_000673"
     CDS             complement(31025..31873)
                     /locus_tag="IXF98_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555006.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(31897..32583)
                     /locus_tag="IXF98_000674"
     CDS             complement(31897..32583)
                     /locus_tag="IXF98_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555007.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(32576..33610)
                     /locus_tag="IXF98_000675"
     CDS             complement(32576..33610)
                     /locus_tag="IXF98_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1555008.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(34005..34343)
                     /locus_tag="IXF98_000676"
     CDS             complement(34005..34343)
                     /locus_tag="IXF98_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1555009.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(34345..34701)
                     /locus_tag="IXF98_000677"
     CDS             complement(34345..34701)
                     /locus_tag="IXF98_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1555010.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(34707..35894)
                     /locus_tag="IXF98_000678"
     CDS             complement(34707..35894)
                     /locus_tag="IXF98_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1555011.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(36085..36930)
                     /locus_tag="IXF98_000679"
     CDS             complement(36085..36930)
                     /locus_tag="IXF98_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1555012.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            37164..38129
                     /locus_tag="IXF98_000680"
     CDS             37164..38129
                     /locus_tag="IXF98_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1555013.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        11297 a         7838 c         6323 g        12816 t
ORIGIN      
        1 aaaaagattt agatgaacag ttttattgat ttaaatagtt catatttttt atttataccg
       61 cttatttact acttgctgta ctagaatctg aagtgaatgt actcaaaaca ttttcaaagt
      121 ctttgtcttt gatttttaca ttagcgtctt tcaatacttc gccaattact ttattctgga
      181 aggtactgtc acttaatttt gtgtctgttg caatttcttt caattctttt ttgtatttat
      241 ccatgtcgtt gcctttgttt tggtttttca ccattttaac cacataatat tccgtagtat
      301 aagttgaagc attggtagaa gtgatcacat cagaaacttg accatctttc aacttaaagg
      361 ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga atcaaatttc acttttccgc
      421 catcttcttt tgtcgtagta tcagttgact tgtctttggc taatttagag aaatcgtctc
      481 ccttatcagc agctttcttc acatcttttg catcatcttc acttgctact tggataattt
      541 gagcttccac ttctggatgg aaagattccc aagccgtttt taaatcttca tcggtcaatt
      601 tgatatgttt ctttagacct tcttgaaagg ctagactttg tttgatactg tctttgaatg
      661 tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa tgaatcaccc aattgtttct
      721 ttgtttgatc atattgagaa tcgatttctt tggttgatac gtcatctcca tatttatctt
      781 caaatacttg ataaacgatc aactgttgca cagcggaatc tgctccttgc atttttttca
      841 agctatccat tacttcttgt tcgctgattg taccgccttt ataactaacg gctgtttttt
      901 caccggcatt cgtacagcct gacaataagc cagtgcctag tacgatggca agtgctaaaa
      961 atcttttttt catttatcaa ttctcctact tttcgttttt tgcgaattgc taaaaacaaa
     1021 ggtgttcaag atgaaaatca tcaagaacac ctttactaat ctgctcgata cgtcctgaat
     1081 agatataagg ctacacaatc cagaaagttc gctcagcaga ataaatgtca agtagcacat
     1141 cacaccttaa tatactactt cttttgttac cgtttttcaa gtctatcttg cgttccgtat
     1201 ttaacgtaac taattcttag aacaaatgtt atcgattttg tttttcttct tccgctgctg
     1261 ggatattcaa atcagacact tttatgttcg attttccaga catttttttg ttgtttttat
     1321 tatgatatat ttttatacaa ctaaaaatga atatattaaa actaccaaaa ataactttta
     1381 acgctgtttc attatatgtt ttttctattt tatataaact tttggatact taataccaac
     1441 cggttggatt gtaccaacat gagaatgtgt cagttctatt tcctgtcgca cttgcatagg
     1501 cccatactcc ttttgttgca tttgctgatc gaatagagtt tccaactttc gcttttgcac
     1561 cgtgacgatt atagacacga ctatagtggt tagaatgacc tgtttttctc gtacaagttg
     1621 tatcactata ataaacccat cttccagagc tatgattaat tgctgttcta gaaatttccc
     1681 ggtctactgg taaatttgac ttaataatat tacctttagc atcaaaatga atctctcctg
     1741 gctttaaaga tttgtaatag atttcaccgt gaactgaatt tgcttccact tcagatgaca
     1801 tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat aataactgct tttttcataa
     1861 aacgacatcc tttccttttt gatttttaaa gcatgatctt cttgtttcct attttggtca
     1921 gatatggatc atgcgtgact acgataacta acgtaccttg ggaagcttgt tgcgataaga
     1981 tttcaactac tttatcctta tttcttttat ccaatgaccc agtcggttca tcggctaaaa
     2041 tgattttgct attcttcaat aagactctcg cacaggcgat tctttgcttc tcaccaccag
     2101 ataatgtgta gataggcgta cttagcttct tgtttatatt tacttttttc aatgcctcat
     2161 actttgctgt tcgcttatct gaaacattta aattcgaaaa cttcaatcca atatctaaat
     2221 taaaatcgat tgtttcatat tcaatcaacc caaaatcttg aaaaagaaaa gatatggtat
     2281 ttctttttat caagcgcttc tctctcccag ttaattgttt cgtctcttga tcgaacaaat
     2341 agaattgtcc tgaactgaac tgatcattca atcctaatat attcaataac gtacttttac
     2401 ctgctccact ttctccaaca acagtcacaa gatccccagc atccgcagtg aaacgatcca
     2461 aatgaagaat cgttttgttt cgaatgattt ttttcgcacc ttttatcact atcaacggct
     2521 ccaattgtta acctccattc atcagcaata actgactttt cttttctgta gacaaaataa
     2581 aaacaactga aagaataaaa tcaatgaagc taaagaaaaa aatattaaaa agatatttag
     2641 gctctctttc tataagaaaa aggaagatta cacttgtcgt tccccatttg atgaggttca
     2701 tcatgaagaa atctttatac gtttcaaaga gcgaataacc acttgaacga aagatagcga
     2761 ttttccgaca attgtagaca aaatatatgc ataacatgac atagctcaac aagaagaaag
     2821 aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa tgatctgctt aacactttga
     2881 cagctatctc ttcagtctga gtatacggaa atgataactg caagttatca cgtaactgac
     2941 tttcttcaat cagctcaaat aacctttctt tcgacaaaga gcctatcttg atctttaacg
     3001 gtggatagat ttctccgctg agaatatttc tttccaaata catactgttt ttcaatgtca
     3061 gtatttcaac aaccggataa tgttcgatcc aggggataga aggaataaat gtataaatgg
     3121 gttgattgtt ttttataaat agaaatttcg gttctttttc actgatttct tgataatatt
     3181 ctgttatttg cgccaggtca ttttttaaat ctgtatgtct atatcgttca gggatcaata
     3241 taacagggtg cgtttctttt tcggttattt ggatcaacct tccagagaca tcaatcaaag
     3301 gaaatttctt aagataattc agatttattt ttatccctct gccaaactgt tcattttctt
     3361 cattcaagta atctgataca ttgactaata tacttccgtt tttattcaaa gtttcatata
     3421 attcctgatc tgctgctgat gcaaaacgat tggaataaat aaaatccacg tggtttttcc
     3481 cgatggttac tggataaaat acatgatagt cctcaccagc gatttctttt ggtggttcat
     3541 gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg
     3601 tcaaaagaag agaaaacaac aatccaaatg taaaaagaat gtattggata tagttgaacg
     3661 cttctcttaa cgaagcgatt ttttcaacta ataaaagaga aatcaaagag ataccatata
     3721 ttccgctgaa caagaaaacg tacatctgga acaaataaag aaattgctcc ttataataca
     3781 taaaaataaa accaccaatg attcctgcaa ttatgaatgt aataatgatt ttctttttcc
     3841 ccaataaaca ccacaatact tgatgtaatg aaaagtcatt attcaacaat aaactgattt
     3901 ctttattcat caataagaaa aaggatgata acaaaaggaa taaaagaatc aatccaatag
     3961 ttaaatacac tcgataatta tatacgtctg tatttgaaat aaccgaatac gaatagctat
     4021 tttcaaagtc ttcaaaaata ttagtggcat aatcagtgtg aaatgcttga ttgaatcgat
     4081 cagtgaatag tgaaatgacc cgttcatagg cttctttttc tctcgtcatc aagaaaaatt
     4141 caccttcaaa atcttttatt ttgatcgatt cttcaatgga cgttacgcga aaggaggtat
     4201 tagaataggg taccttctcg tgagaagatt tgacatcccc ttgataatac aatgtcattt
     4261 caagctttgg ctgtatcttg aagttccaag atgtatctac ttcattggat tcatttactg
     4321 tttttttcat ataagaaagc tcagtttctg atgagacatt ttcaaatacc atgattagct
     4381 gctcatattc ttcttgccca ataaaatgtt gtggcaatcg aatcggagta taagtatctg
     4441 tttgatggaa aaattcattt gtccgataga gtacctgttt atttatacta accaaggcaa
     4501 aaccactcat gataccggtc actatgattg tgagtaaaac tgtgaacttt tttttgatta
     4561 tcaccactct cccttactta aatgattctt tgataaaatc ataccgtttt tcatcaaaaa
     4621 aacgtatcta tcaagtgtat aggtcatgta gaataaatct atatactgta gaatcgaaag
     4681 gagaagaaga tgaacaccgg agaaaaaata cgaacttacc gcagaaattg taagcttacg
     4741 caaaaagaat taggcgaaaa aattggtgtt tccgataaaa cgatttctag ttgggaaaat
     4801 tctcgtacaa tgccagactt tgaaatgctg tcacttttac atcatacttt aggtctgccg
     4861 attgattttc cttctattga atcagctacc gaatcaatag aaccaacatt gccagatgaa
     4921 cctcttgtta acaagaaatc ttattcatca aaacaaccct tctcactcaa aaagacacta
     4981 tctttctttc tgctcgtctt ttgtttggtc tatctttttc ttatcaatta tcaatacaaa
     5041 aatgaaacga tcgatcgatt caatcctttt ttgaaaccac aaatcggcta taccacactc
     5101 cctgtaggat acgtggacaa ttccatcgaa cgttgggtat gtaatgatat ctttggccat
     5161 ggtacatggc tgtcttttca cgctggagag gtagaaaaag agaaaaacca tgctgtaatc
     5221 aaacacaaag gaaaacacgt catgaacgtt cgcctgctca cttgggaagc cattccctca
     5281 gattaccaag aaatcctcag ccaagactat gacgacgcct taaaaagaag ccctcaggag
     5341 ccagataggc attaagtagg agatgaacgt agagatagaa aaaatcaatg cacatgatct
     5401 tatccaaaaa acaggggtac aaattaattg aaaaagcctt gagatgaaag ttcctaaata
     5461 aaaaagttca gaatgatagg tatttctggc ttatagtata atatttacgc cacagcctcc
     5521 caatgattct ttttggaatc ataatcaagg atggacattc acaaaaatat tttgctcatt
     5581 cgatccgaat agattaacga ccaatctagt aagtaatata gattggtacg tagtcaagct
     5641 gcacataatt tagatcaatt ttagttcttt tttatatttc tatgatctgc tcatttactt
     5701 ttatatgatg gcttacagta acataaataa atatgataat cgctggtagt ttatccaaga
     5761 gtactcaata aaatacaaaa tggattgttc aagcgcctat cataatgata aaatgaaaca
     5821 aataaacgat acaaggagga agagactttg agtaatggca gaacaatttt ttcattgtaa
     5881 aaattgttca gttgttaatc tatgggattt cggtagcatg ggcatttttt tatagaggaa
     5941 tcacaccaac ctggccgttt tatctcttgt tagtgggaat attagtagga aattttgccc
     6001 ctcttcacta tcgtacgaac ttttttccaa ctgattctgg cctttttctt aaaacaaaga
     6061 cgcgaaaatt agaaaacatt attgaaatga caattacctg tataatcatt ttcttagtta
     6121 tattcctaaa cgtcttctaa taagaatctc ttaaaattag cttgttacag ttagttacct
     6181 agtttatata acacagttat cttatcttcc ttatcaacat cttttgccaa ctctttgtct
     6241 taaattcgaa acatataagc agagctacga acaccaagac tgtttcggct ctgttttttg
     6301 catcaaattc actcttatat agctgataat ctcggctctt taaaagaaat tattttcgct
     6361 tcattttatt ataaaaaggc taggaacctt gatgttatca ggttcctagc ctttatttaa
     6421 gtattacccc acagaccctt ccatatagat ttttaaatta cacactagtt taaactaaaa
     6481 caaacaagga aaaacttacc atttaatcaa aaaattagct catatgaata tattagaatg
     6541 taaattagac tggtcaaaat ctggtcaaaa aagaggacga atacaggtag tcctcttttt
     6601 tgttattttg atattctaaa tcatttttta ttggattatt cccttgttcg atatagaccc
     6661 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
     6721 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
     6781 aaaacaagcc cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg
     6841 tatatttttt tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc
     6901 ctactagtta gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt
     6961 atctgaaaca gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt
     7021 cagatacatt tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag
     7081 cctatgcttt ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat
     7141 gaatgaaaaa ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat
     7201 gtgaacaaat aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg
     7261 aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac
     7321 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc
     7381 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
     7441 ttaggaattg gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca
     7501 gaaacaaacc actcattgga aaagacatga atcaaatcat cactcgcttg tgtaaaatag
     7561 cgacgtttaa tgacaaagat atcttcatta ctatctaaag tccccacatc aaatgcattt
     7621 aaaatttgtc ggtaaacttc tttgactcct tctgttttat tcaatttagc caaagctttt
     7681 tcaatttctt ctcgtgcatc tgaactattc gcagcataag ttcctagtaa ttgatcaata
     7741 taagttgaat caatatcata caacttagtg gtattttcac tataaaattc gatcgttgaa
     7801 aaatcttctc cttctttttc tggttcttcc tctattaatg aacccttcac ggtctcgtac
     7861 acgccaattt gttcttccaa aagttgaatt tgattgttca aagttggaga tttcaccatg
     7921 tcatcattgt aatcgtcata agtcactaac gattcataag cacgattcaa ttcttgaaat
     7981 gctttgacgt actcgatacg gatttcaagt ggcgtatgtt catcaatgga ttccatggat
     8041 gaaacctctt ttaacttacc atatgcttgt ttgaatcttt ttttggattc ctgataactt
     8101 ggataaatca aacctgattc ttcttttgct tttgaataca gttttgtcgc atcctcgaca
     8161 ttcttctcca ttgtcgctgg cttacgaaac gtcacaatca atccttttgt cttatcagga
     8221 tacgtacgat tcgtacgtga aaaagcttgg atcaatcctg catactctag attacgatcc
     8281 acaaataggg tttgaatcgt aggggcgtca aaacctgtta atagacgatc cactacaatc
     8341 gctaaatcga tttgtttccc aaattgtttg aattccgctc tttttcgtgc caaacgatta
     8401 ttgatgtctc cgttataacg ctcaatgtct gctaatgacc aggaagtatc ataatactga
     8461 ttatattcct caataatttt ttgcatttct tcttgttggg cacttgaatc tcggctattt
     8521 tcttctaatg aataggtaat agcgattcgt ggaaaatcag gatcctccat cgttcgtcct
     8581 tcacgaatga gctgattggg aaactctttt gttaaccaat caggctcttt cgtcatctct
     8641 ttgatggccc gataatactt tttggccatg tcaatggaac tcgtggttaa gattgcagat
     8701 ttcgttggcc tgccatttcg aaaatcaaat tttgtatagg cattatctgg acggaacatt
     8761 ttatgaatga ctttttggat atgttcatct gcttcaaaaa cagctgggtc caaataactt
     8821 tcttttttga caggttccat ttgatcaatc atacgattga tctgttctga tgaatactct
     8881 gcatatgttt ctacttcttt taatttccga tagattttat tctctagtga agtcggctcc
     8941 acggtgtttt catgctctac ttgaaatcct aagacagagc catcttctag cgcattctta
     9001 atggtatagg tatgcaagac ctctccatac tgatcatggg ttgtacgtgc caattgccct
     9061 ttggcttgtt tccgattctc aggaaaaatc ggtgtacctg taaagccaaa ccaagtggat
     9121 ttcggaaaca tttttttgat ttcttccata ttttcagcac ttaacgctcg atgacactca
     9181 tctactacaa acacgatatg ttggcccatt aatttttgga agcgattggt tccttttttc
     9241 tcttcttgtt tctttgcata tttcaacgcc gcatctagtt tttgacgggt tgtaatgatt
     9301 acaacatttg cattggcatc tgccaacagg gtttcactta attctttggc acttcccgtt
     9361 ccaacgatca agctattcgc tttggcgttc ccggaagaaa tcccagtatt aaattccgaa
     9421 gcaaattttg taaactctgt ggtggtttga ttatctaaat ctttgcggtc cactaacatg
     9481 atcgttcgat cgatccccga ttttcgtgct aaaagcttag tagatacaaa actagtcaac
     9541 gtctttcctg aaccagtcgc atgccacaca taccctgatt gatgtttatt tgcggcaata
     9601 aacaaggctt gaatcgcatg tacttgataa ggatgtaaca ccatgagggt tttattatct
     9661 tgatcttcac tgacgattgt ataatccgca atcaaacgat gagcatccgg gatatttaac
     9721 acttgtttac aaaactcata taagttttct actttttcat tgtctttcgt tcgccaacta
     9781 aataaaaact tagggtgcaa gtctttcggt aaggcatttg caaaataccg agtggtttgt
     9841 tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat tgttccgaaa cataccttct
     9901 tctgcatatt ttttgatttg gttaaatgcc tgataaacac catcttttgc agttacttgt
     9961 ttcagctcga tttggacaat cggtaatcca ttgattaata aggtgacgtc aaaacgtcga
    10021 tcccgtgctt ctatgttact tccacgtttt gcaatttgat ggaccacttc ataagtagag
    10081 attcctccac caatgtcttg attggaataa aggataagtg agacagaacc caattgcgaa
    10141 tcttcccgtt caatcgtgat ccgagccatc ccattttcac cttttaacca tttcgcagca
    10201 tcaaatgggg ttttcgttcg cagcagtaat tccgttttga tcgtttcaaa ttctttatcg
    10261 gtcaaaggaa aatcattcag ttctgcttga ttatttgaga taattttttg gcgtaaattg
    10321 acccaaaggt cttcttctga ttttagatcc ggacgatacg tccactgatt atgcccttct
    10381 ccaagtacac gaatcaactg ttcttcgact tctgcttcct cacggtgact aattttcatt
    10441 cttcttccct cctagacaaa catcttttgt aagaagcttt ttttcgtttc ttttagtaaa
    10501 tctaacttac gttgatgaag agcgatcgta tcatctagct gtttgaagaa agtaccgatt
    10561 ttttgttgtt catcctttac aggaacctta acactgatat tctcaatatc agaactgtta
    10621 taagaaggca atgcccctac ttgggagtat cttggcaaat ttatagtttc aaacaatatt
    10681 ttaccaaaat agatatccca agtattatta aataaataag ccatggtatt gttatctatt
    10741 aagaaagggc ttgtgaccaa acgttttcta ttaagcatta ttgctgcgcc aactttagca
    10801 aatataatcg ctggcacatc ttttattggc ttccattttt tacgctcaat ttgctcatta
    10861 gatacataat gatttgcatt tctcatttca tactcattac caatgttatt catgtcggaa
    10921 actttaaaaa atggagtacc ttcagaacca ccttgctcgg catcaggaaa tccaattccc
    10981 gattgagttt tacctaattc tcccaactta cgctcttccc aatcttccgt aaaaccagga
    11041 aaccggattt ctggcacttt tgctccgttt tcagggaaca ttttttgtaa gaatcctttt
    11101 ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatagtgtc gtcgagtttt
    11161 ttgaagaata tgccgatttt ttgttgttct tttaaatcat tgggaatata gaatactgta
    11221 tctgaaactt ttttccagtc tgaacgaggc atttttgttc cagatgtatc atttgcagcc
    11281 ttttgatatc tatcagcttg aataagagaa taaataaagt ccgagtcgct cttcttagct
    11341 ctaaaaaccc agaaatcccc aatcgctacg cctgtaaaag ttgccttgag ccaatttttt
    11401 aaatatggac gtaactttcc atatagaata tcatttggta aaaattcaat cccttttcta
    11461 ttatcaaatt tctggctcac atctttattc agtctacctt ctcctgccac gatatcttca
    11521 aattcaactt ttggaagtct actggagttt gtacttttat taagtctaac aactaaatca
    11581 attaacttac gctcttccca atcttccgta aatcctggga aacggatttc tggtcgtgta
    11641 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacatttcgt tttcaggtgt
    11701 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
    11761 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
    11821 atctacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
    11881 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
    11941 gttttcttcc gatagtttat tttgattttt ccctttaaca aattcacgac tagcatcaat
    12001 gaataataca tctcgagttt gacgattctt cttcaacaca atcaccgtcg ttggaatcga
    12061 tgtcccaaag aacaagttcg caggcatacc gatcacagca taaatactgc catcttctag
    12121 taatttttga cggatgaccc cttctgcagc gccacggaat aaaactccgt gtggtaagac
    12181 gatggccatg gtacctgttt ccttcaaatg atagaaccca tgaagaagaa acgcaaaatc
    12241 tgctttggat tttggcgcta atttcccata acgattaaac cgagaatcat ctaagaaagt
    12301 cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat caaatgtgta
    12361 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
    12421 ggcgtcgaca ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
    12481 ttcttgccca tgatatttaa cgttatctgg gtgtgttaag tagttacgta cgtttaacat
    12541 caatgaacct gaacccatcg ttggatcaaa aacactaaag aatcgtcgtt ccttttgatc
    12601 gagcgttacg atttgcgcca tcatatcaga aaccatatga ggtgtgtaaa attctccggc
    12661 tttcttccct gcttctgaag caaattgact gatcagaaac tcatacgcat ccccgatcac
    12721 gtccccatcg tgttctaaaa catctacatc attcaatttc ttgatgactt cggtaatcgt
    12781 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
    12841 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
    12901 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtgc
    12961 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
    13021 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
    13081 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
    13141 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
    13201 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
    13261 taaagttgct atataccctc ttattttagc ataccccctt ttataaaaag caaaaaaaaa
    13321 agaaagaaac ttattcttgc taataaacta cgcgaaagga gtctaacgtt gtttgatata
    13381 acttagactg atcgttaact tattttttat atttctacat agttatcttt agaaccattt
    13441 cagatacata tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag
    13501 tataaaatat acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta
    13561 aaacacttat atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa
    13621 tatagtcggg ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga
    13681 gatgtataag tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga
    13741 atcattattc aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa
    13801 ataccacaga ataatctttt tacccatttt caagtaaccg aagtggaatg tctttaccaa
    13861 cttgctctgt cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt
    13921 gagcatctgt agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa
    13981 gcttccaagt aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta
    14041 tcgtcttccc agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca
    14101 tcgtctgctc cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca
    14161 aacttatata ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa
    14221 attcactata accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa
    14281 aagaaaaata gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact
    14341 ctacttgaga ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat
    14401 ccttattatt aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg
    14461 aacactttac cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat
    14521 tttccaagga atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat
    14581 attttcttca ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga
    14641 tttgctgagg cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac
    14701 tctttcggaa aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct
    14761 tccttcttaa tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc
    14821 atcgaatctt tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc
    14881 tttagtgctc ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt
    14941 acaatgtgaa acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg
    15001 ttgatcaatt ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt
    15061 tcaatcagca ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc
    15121 aagtgatcaa ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg
    15181 gcataatcga taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt
    15241 ttaatgtaat ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt
    15301 ttgtcagccg gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa
    15361 attacttctt tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt
    15421 actctcatta gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat
    15481 accaaaagaa gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt
    15541 caacatatat aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg
    15601 ctgaccaagg taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca
    15661 gaatatgttt ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa
    15721 ctatgtcggg tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt
    15781 acaatttcct caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt
    15841 tatctacata aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt
    15901 gatcccccgc tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag
    15961 ttgaataata attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct
    16021 ctgcctcaat tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt
    16081 attttccccg aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg
    16141 ctgcaattgc atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg
    16201 gaccgatttt tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta
    16261 ataaaacatg gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg
    16321 gcaaaccaat cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat
    16381 agttcacctt gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga
    16441 tagttgctcg atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt
    16501 acatggtgac aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa
    16561 aagaaattcc cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga
    16621 tggaaaagcg aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt
    16681 gatggtaaaa atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt
    16741 caataataga cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa
    16801 tggaggcacc ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa
    16861 tcctctcctt aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca
    16921 ctaattcctg cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca
    16981 gcgtttctaa tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct
    17041 cctgctttta caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca
    17101 tggatcaata attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa
    17161 atagtaatga cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct
    17221 acgccctttc ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac
    17281 acggttcctt ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat
    17341 tcatcagaag tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg
    17401 attacacacg taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt
    17461 aaggctgcta agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac
    17521 aaaccagcaa ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt
    17581 actccataga gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct
    17641 agctgcttca actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga
    17701 gcaatattag aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat
    17761 tgcatagtag cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc
    17821 aaaccaccca tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc
    17881 attgctataa gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc
    17941 cccataatta aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt
    18001 cgatccgcaa agggctctac ataggacatg atccaaacag taaagataat cggtaaaact
    18061 gatcccgcat aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta
    18121 gaaaaaagct gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt
    18181 gggttgacat caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag
    18241 gctgcatcag caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca
    18301 gtcaaagtca agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc
    18361 atcggtgtaa aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg
    18421 tctgaagagg ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct
    18481 gtttggactt cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt
    18541 acaccaagtg ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa
    18601 cgcagtctgg tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc
    18661 aatacatttc ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa
    18721 gaccaagtca aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg
    18781 cataatcagt aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga
    18841 tagcgcaatc aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat
    18901 aacagttaga tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata
    18961 tctaatatta actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat
    19021 cgtgaaagca tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg
    19081 aaaaattgat gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca
    19141 acaatagtga tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt
    19201 ccgagatata aaggtatcaa tagaatgatt tgggctagta ttcttaaaaa ttaactagca
    19261 aatgtttaaa ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta
    19321 tcaataaaaa taccaaacat atccataata attttttctt tttcatttct tttatattgt
    19381 tataacagtg aataacataa aaattcccga caatcattgt aatatttttt tgtcacctgt
    19441 attagtattt aatttatagg aaactataaa aactccctag tgaatttaca cctttgggag
    19501 ttcctgtttg gacggtatgc ataatacatt taaatcaatt taacattaac acctcagcat
    19561 atggtatctc cacatatata cggtctttct ttggatagta ctcaatattt atgttgatag
    19621 cacttccatt gtctaatata gctttagccc ggtatgtgtt atctccattt tcatgagtaa
    19681 tcgtcacatc atcacatgat cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca
    19741 atggttttga tgacttctct aatccagatg tacctgtcat ttttgaaaaa ataataccta
    19801 taatagccaa gacaacaata cctataatga caaacttctt gttcatattt actttattca
    19861 actttttatc tcctatctcc aaaataattt gttcttatcc ctcttatcag tacattgaca
    19921 tccctaaaag tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca
    19981 ttttctcatc tttttctcga tataagtagc ccataataac agcaacagca ccaaatacaa
    20041 caggaataaa tgctaacgat actaccatag aaatccaacc aataatcaaa tacagcttcc
    20101 ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt
    20161 tctttactgt gtctgttccg gttgtctcat ctgtttcttg tagtgctcca caaaactggc
    20221 aatacttact ttcatctgtt atttctttgc cacattttct acaatacatg tcttctcctc
    20281 ctaaaatcat tcaattgctt attagttaac tgtttacttt attaaataca tcagaaaaca
    20341 gctcattcat tggtacactt aatatctcag aaagcatctt catttgatag ggtttaggaa
    20401 tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt ttttttgcta
    20461 actccgtttg agaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
    20521 tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac tcataaaata
    20581 taaaaaactt caaataccat tacaaataca acaacttgtt gtactatact gagatggggg
    20641 tgtattaatg aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg
    20701 gtggacacaa aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg
    20761 ggaaaattct gtaacagagc ctaacctcga tagcttaaca gctctttcta gagccctagg
    20821 aacccctgta agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc
    20881 tctctatcgt tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt
    20941 aaaaaggcaa gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc
    21001 gaactttaac ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct
    21061 agtttaatac aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac
    21121 ttgtaaattg atacagagga actatcccag ctcctgataa tatcaattct tttattgttt
    21181 catctatccc tgcccctttc gctaaatata ttaaaaaact actacttgct ttcacattgt
    21241 atagtttctt aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc
    21301 ctctgttttt tattaaagca tttttcacgc tgttatcccc aacattacgt aggcgttcac
    21361 aaacagtgtt aataagttct ttctctgtca ttagatatgt cccttgccac gataagccct
    21421 gtaaaacttc atgtgcttca tctaaaatca attgtttttc cttgctgagt tctgatcggg
    21481 aattttgagg aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat
    21541 aagaaaaata ttgatatgta aaatgaattt tttccagaag tggaaggata tcttcattca
    21601 atttttgaat cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat
    21661 gattaaagtt tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca
    21721 aaggaacatc ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta
    21781 acttattaca tttatctatg tcataagttt taccatagta agaactcatt tttttagcta
    21841 attgttcccc cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat
    21901 cagaaagcaa gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga
    21961 aaagtttgtc tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt
    22021 ttcgaaaatg tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc
    22081 gactgtcttt ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat
    22141 tactggaatg acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg
    22201 gattatgatc gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca
    22261 tatcaatggt taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca
    22321 ggcagtcttt aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa
    22381 aatcggtttc attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac
    22441 atgtcatggt aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta
    22501 ttatcactgc atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt
    22561 catcacaccc ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt
    22621 cagtaattgc ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc
    22681 gaatactttt ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt
    22741 ctatgcttaa gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt
    22801 taacagtgtt atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat
    22861 tgcctttatt ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa
    22921 actcatctct tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt
    22981 ctttttttgt aactggcttc tctaataatt tttcttgatc tacataaatt ggaatattaa
    23041 tcattgatgt ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac
    23101 agataataaa aacaaactct ttattactat atgttttagt cttatttgtt tactcaacct
    23161 ttataccgtt ccatgaaact atcaatatca gattttttaa atctaatgac cgtatcaatt
    23221 tttataacag gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct
    23281 agatattcac ttgcctgctg atagtccata tactctagag tgactccaga aataatgtct
    23341 tctagcatag gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa
    23401 acaatgctat caatgatttg tagagacata tttccccctc ctttcgtgat tgcttgaaca
    23461 aattttctaa gaattatctt cgtatttttt gctcaaaata aaagtatttt gttattaacc
    23521 gattaatcaa acaaaaaatg attaccagtt catcgacata cacataatac ctcgataaaa
    23581 aaggggtgtc aacagctttg taaaatttat ttacaacacc ccttttttga ggtatactaa
    23641 ttttaggagg tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa
    23701 agatactcgg aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat
    23761 ttggtcttat gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga
    23821 agggaaccta tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga
    23881 gtatagagga gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata
    23941 gagatagcat tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc
    24001 gtcaacagtt cccaccagta cactctaatt tagatacatg gctggataga tattccaaat
    24061 tagaaccaga gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa
    24121 aaattagtct tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaag
    24181 aatttgacaa taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg
    24241 tagatgaatg gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag
    24301 aaattactga taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca
    24361 ctaagaaaaa aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag
    24421 aggagatgat ttaatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg
    24481 gatgtataaa ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag
    24541 aagaggtttt agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat
    24601 attatacggt gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg
    24661 gataactcat cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc
    24721 aaataatcaa atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata
    24781 ttgtcaaaaa gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa
    24841 acagactgca caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat
    24901 ggaaaaaaca cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa
    24961 agaggagctt aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc
    25021 cttcttcaga ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg
    25081 gaaagacata gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga
    25141 atttggtaaa gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt
    25201 agatactgag actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt
    25261 aaaacttgga tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac
    25321 tctatacatt ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt
    25381 gcctcgtatt acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg
    25441 agaatctgtc aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga
    25501 catctatgca catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat
    25561 gatggctcat cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac
    25621 aaaaaagcta gaacccttat atatcaaggg ttctagctcc tataacaggt ttacccaaca
    25681 ctgccttcca tctcataact gatcaatcga ttgagttcga ctgcatattc cattggaagt
    25741 tctttggtga atggttcaac aaagcccatg acgatcattt cagttgcttc agattcggtc
    25801 aggcctcggc tcatcagata gtagagttgt tcttctgaga tttttgagac tttggcttcg
    25861 tgttctaaag agacttggct gttgtggatc tcattgaatg ggatcgtatc ggatttcgac
    25921 cattcgtcca tgatgatcgt gtcacattcg atatgagaga tcgaacccgc actgtttttg
    25981 gcaaaggtta cttgtcctcg atagtttact tctccgccgt cttttgaaat tgatttagag
    26041 acgatagagc tagatgtatt tggtgcattg tggatcattt ttgcacccgt atcttggatt
    26101 tggtttgctc ctgcaaaggc gatggagagc atggtgcctc gtgcaccttt tccatctaag
    26161 taaacacttg ggtatttcat cgttgttttt gcacctaggt ttccgtcgat ccattcaacg
    26221 gttgcgcctt cgtatgcttt tgctcgtttg gttactaagt tgtacacatt gtctgaccag
    26281 ttttggatcg ttgtgtaacg gcaatacgca tcttttcttg taaagatttc tacgatggcg
    26341 gcatgcaggc tgttacttga ataagtaggt gccgtacaac cttcgacata gtggatgctt
    26401 gctccttcgt caacaatgat cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc
    26461 cggaagtacg tttgtaatgg aacatcgact ttgacgcctt ttggcacgta aataaaggtc
    26521 ccacctgacc atacggctga attcagcgct gctaatttgt tgtcggttgg cggtacaagt
    26581 tttgaaaagt actctttgaa taattcaggg tattctttca aagcagagtc agtgtcagta
    26641 aagacgattc ctaacttttc gaattcatct ttcatattat ggtacacgac ttctgattca
    26701 tattgtgcgg aagcaccggc tagataagct cgttcagctt ctggtatacc aatcttttca
    26761 aatgtatctt tgattttttc tggtacgtct tcccagtcac gagcgggttt gtcacttggt
    26821 ttttggtaat atttgatggc atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc
    26881 attggcattt tgttaaatgt ttctagagat ttcagacgga attcaagcat ccattctggt
    26941 tcgcctttga ttcttgacat ctcacggatc acttcttctg tcaagccttc tcctgtactg
    27001 aaaaccggtt cgacatcatc gtgaaacccg aatttatatt cttctaattc tggtacgccc
    27061 atgttctcac tcctcttcct tgtggtaatg atggaaagct gcggtaccat cgttttccat
    27121 tgcttttcct agtgctttcc aggcaagtgt cgcacatttg atccgagcgg gaaatttagc
    27181 tacaccacca agcatagcag catcgcctaa ttggtcggga tctttgactt cttttccttg
    27241 gacaagctgt agaaagtcta cagttaaatg ttctgcttct tctattgatt ttccgatgac
    27301 ggcgtctgtc atcatcgagg cacttgccgt actgatggag cagccactgc cgtcaaaggc
    27361 gatatttttg atgattccgt cttttacatc gacttgcaat tcgatcacat ctccacatgt
    27421 ggggttgttc aattcgatct tgcttgtcga gtcagtcaag cttccacgat gatgcggatg
    27481 ggaagaatga tccaagatca cttgacggta taaattgtct aatttagata gtcccatgtt
    27541 ggaaaaactc ctttgttgcc atgattgctt caactaaacg gtcggcgtct tgcgttgtgt
    27601 tgtagaaata aaagctcgcg cgagcagtcg ctgatacttc aaggtacttc aatagcggtt
    27661 gcgcacaatg atgacctgct ctgacggcta ccccttccat gtctaaagct gtcgcgacat
    27721 catggggatg taacccatcc aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt
    27781 gtggaccata aacagtcaat ccatcaattg cttgtaattt tggcaacaca taagcggcta
    27841 gttcttcttc atatcgatgg atattgtcca taccgagttt ttgcaaataa tcgattgcat
    27901 ggcctaatgc gatagcgccg gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt
    27961 ctttccatgt actgtcgtat aattcgacaa aatcgatcat ttcgccacca aattcaacag
    28021 gttccatttc ttctaaccat tttcgtttac catataaaac accaatacct gttggtccgc
    28081 acattttgtg tccgctgaaa gcaaagaaat cacagtctag tgcttggaca tcaatggcca
    28141 tgtgcggggc agattgagca ccatctacta cgatgacccc gccgttttca tggacaagtt
    28201 cagctaattc ttgaacagga ttgaagacac ctaagacatt ggatacatga gcaatggaga
    28261 cgattttggt tttgtccgta atctttttcg ctgcatcctc catatccagg aagccttctt
    28321 ctgttagttc gatatatttc aagacagcac ctgttcgttt tgccaactgc tgccacggga
    28381 taacattaga atgatgttcc atgtaagaga gcactatctc gtctcctgct ttgatatagc
    28441 ggtcgccgaa ggttctggct acccaattca agcttgtcgt cgtaccacga gtaaacagca
    28501 cttctgctgt ttcttttgca tggatgaacg cccgtacttt ttctcttgct tgttcataat
    28561 catgggtcgc gcgttctgct aacgtatgga caccgcgatg aacattggcg ttatcgtgtt
    28621 cgtaatagta cgctaatgcc tggagtactt gttttggctt ttgggtcgtc gctgcattat
    28681 ccagataaac taaaggttca tcattgacgt cttgaaataa gataggaaaa tcattgcgta
    28741 gctttttagc atcgatcatc cattcaactt cccttcgatt acttcgacta attctttccg
    28801 aacttgttct acagggattg ctgttaagac agaaccgagg aatcctcgaa tcactaagcg
    28861 ctctgcttct tctttatgca atcctctact catcagatag tacatttctt ctggatcaac
    28921 tcgtcctaca ctggcagcat gtccagcagt tacttcgttt tcatcgatca aaagaatagg
    28981 attcgcatca ccacgtgctt tatcagaaag catcaagaca cgactttctt gttgggcatc
    29041 tgcacctttt gcccctttca agatgtggcc gataccatta aaggtcaatg ttcctttttc
    29101 tcggatcact ccgtgttgca aaatatgccc gatcgtatga ggggctttat tggttactcg
    29161 tgtatcgatt ccttggattt gtctgcccga gctgattgcc acgattttga cttctgcgtg
    29221 tgcaccttct cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac
    29281 accaattgcc cagtcgacag aagcatctcg taaaatgtgc ccacggcgat tcatataaga
    29341 agtaatattt tctcctaatt gatctacggc tgaatatttg atttttgaac cagctttagc
    29401 gatgacttcc acgatgatat tacctgatga ttttgctact tgttctttgg ttgtctggaa
    29461 acgttccaga taagaaaatt cactatgttc atctgccacg atcaacacat gcttgaagaa
    29521 gtgctcatcg gatgcgccat cttgaatgaa taaggactcg agtggttcat cgatcacgac
    29581 atttttaggc acatacagga aaataccgct gttcataaaa gctacatgga gtgcagtcaa
    29641 ttggtcttcg ttcatgttta ctgctttttt catataatat tcttggacta gttctggata
    29701 ttcgatcatt gcagtgaaca agtcggtaaa aatgactcct ttttctgcta attccacagg
    29761 cagttgttca aaaatcgtcc atgaaccttg ttggacaagc acaggattgt ctttcattgc
    29821 atcaaatgcc ggaatcgtgc ctgtttcact gatggtttgt gtttctttca catcaaataa
    29881 cgaccagcga tggaatttca cccgatcgat atgcggcaat ggcagttcat ctgctttcgc
    29941 tagtgcgtct tgacggaagg ttctcatcca atcaggttcg cctttactta aagaaaaatc
    30001 atttaccgcg tcaagataat cgagccagtt tttttctttc attcttgctc ccccttacgc
    30061 ttcttcttct ttatagtcga ttcctaattc tttactgata ccagcgtagc cttctgcttc
    30121 cagacgttta gctaggtcag cattccctgt catgactact cggccttcca tcatgatgtg
    30181 aacgacatca ggtgtgatat agttcaataa tcgttgatag tgggtgatga tcaatgcacc
    30241 gaaattgtct ccgcgcattt catttactcc tttagcaacg actttcaaag catcgatatc
    30301 cagacctgaa tcgatttcgt ctaaaattgc aaatgtaggt tctaacatca acaattgcag
    30361 gatctcattc cgtttctttt ctccgccaga aaaaccttcg ttcagataac gttcagccat
    30421 ttcttctggc atatttagta aagccatttt ttcatctaac tttttcaaaa atgccataac
    30481 agaaattttg tcgttttcat cacgtttcgc attgatcgct gcacgcatga attccgcatt
    30541 cgtgatacct ggtatctcac ttggatattg catagctaaa aataagccaa gtcgcgcacg
    30601 ttcatctact tccaagtcta aaacattttt cccgtcaaac aaaatttcgc cttctgttac
    30661 ttcatagttt ggattgccca tgatagcggc agataaggta gattttcctg tgccgttagg
    30721 tcccatgatg gcatggattt ctccggtttt cattgttagg tttacgcctt taaggatttc
    30781 tttttcctcg atcgacacat gtagattttt tatttctaag acagacatgc attatacccc
    30841 caaattctct ttcgttctca ctcattttag actaattgag aatgaagcgc aatgaccttc
    30901 cttactaaaa tcaaatatct tttatttatt attaaacaat tttgatttct aaattagaat
    30961 aggtataaaa aagatgagaa ataagccatt acagacttat ttctcatctt ttttgttcca
    31021 taccttattt tgcatcagcc tttactggtt taacagagcc gccccatttg tctgtgatcc
    31081 attcttggac ttctttgcgg tgaagcactt caatcagttt tttgattttg tcactgtctt
    31141 catcaccttt acgagtcgca ataatattca cgtaaggaga gttgtccttt tcaagcaata
    31201 cggcatcttc taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta
    31261 ggtcaccttc atcattttgg tacgtagaag ctaaaagtga tgggtcgatc gtatggttga
    31321 acttcaagtt cttagggttt tcttcaatat catcgaatgt tgctgtttcc agatctactc
    31381 catctttgac tttgactaag ccagcatctt gtagaattgt gatgatgcgt ccccagtctg
    31441 attcagaatt tgaagtaatg attgttgcac catcttttaa ttcggaaaca tctttgactt
    31501 ttttagagta caagcccatt ggttcgatgt ggacagcacc ggcattgaca aagtcatatc
    31561 ctttttcttt gatttctttg ttcaaataag gaatatgctg gaagtaattg gcatcaatgt
    31621 cgccttcttc caaagattgg ttcggtaata catagtcatc gaatttgacg atttcaagat
    31681 cgattccttc atctttcaat aatggtttca catgttccaa aatctctgcg tgcggtgtcg
    31741 gtgaagcgcc gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc
    31801 tgttgttacc gcatgctgct aatcctacag tcagcaaaag cgctgctgca aatccaaaaa
    31861 ttttcttttt catatcctac atccccctat atcctattat cttttgtctg ttttcttagt
    31921 taagaaatca ccgattcctt gaacgataaa tacgattagt aaaataatta aagtcgcaac
    31981 gaatgtcact gttagattac cacgctggta gccttcctgc caagcaagac ctccaagtcc
    32041 acctgcgccg atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt
    32101 taatccagaa atcaatgcgg gaatactttc aggtatcaag accttccaga tgatttccca
    32161 gtaacttgct cccattgcat ctgctgcttc cagtacacca gggctgactt cacgaaaagc
    32221 aatctctact agacgggcat aaaatggtgc tgcggataaa acaagagctg gaatggctgc
    32281 ttgcgcacct agcatagtgc ctacgattgc tttagtaaat ggaatgatca aaaccatcag
    32341 gatcataaat ggcgttgacc ggaaaatatt actgatgatc gatacgattc cataaatgat
    32401 tcgagcatac ggtttgtttt tgcgtccaat agagtaaagg atcaatccca aaactaatcc
    32461 gatgatcgtc actaagacca ttgagattaa tgtcatatat agtgtatcaa ggatagcctg
    32521 attcatcgtt tccgggttga tttgtttaaa atctagatat gtttctgaaa agcttttatc
    32581 cacgatggat cacctcaatt cctactcctt gttctttgat gaacacttct gcttgatcta
    32641 attctttggt ttcaccagtc atcaagacag tcaatgaacc aaaggaacct tctttcactt
    32701 gtttgatctt tccatacaca acgttgacat cgatgttata gcgtcgaatc gcttgggaaa
    32761 tgactggctc gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat
    32821 tttctttgat gagttcagct aatagttctg tcgaatcagc ttctggttcg atgtcttgtt
    32881 gtacaaagcg tttggttact tcttttttcg gttgtctgaa gacagtcaat acgtctcctt
    32941 cttctacgac tttccccaac tccatcacag cgactttgtt gcagattttg cggatcacat
    33001 tcatttcatg tgtgatcaag acgatggtca aattcaattt cttattgatc gctaacaata
    33061 agtctaatac ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca tcacaaagca
    33121 ggatctcagg atcatttgcc aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg
    33181 aaagttgtga aggatatgct tgtccgcgtc cttctaaccc gactaaacgc aacagttctt
    33241 ctgcccgttc tttacgtttg ctctttggta cgccagctaa ttccagcggc agctggatat
    33301 tttcaagtac agttcttgac cataacaggt tgaaatgctg gaagatcata ccgattttct
    33361 ttcggaatgt acgtaattct ttgtttttta attttgtaat atcttgatcg tttacgatca
    33421 cttcgccgct cgtgggaagc tcaagtccat tcaggagacg gactaatgta ctttttcctg
    33481 cgccagaata gccgacgatt ccataaatat cccctttttc aacgtttagt gaaacgtcat
    33541 tgacggcttg gatgtttccg tttttcccat tgaatgtctt tttgacattc gttagttgga
    33601 ttaatgacat gccattcaca tctttctctt atgatttttc aaacaaaaaa gccttcgccc
    33661 tcttgcctta agcaaaagga cgaaagcttg aacttccgtg ttaccacctt gatttaccgt
    33721 tacttcgcaa taacagcctc ttccagtact tggacttagc ttctttccgt atactgtcgc
    33781 gctgtaacgg gcactcccgt agtaagtttg cttacgctcg cttactctgc tctaagacca
    33841 tcttccatct cattttcgtt acccattttc agcaaacagg gctctcttca aacgtccgtg
    33901 acatgtactc ttcttttcaa agctttctcg tttcatatag gattgcttat gattatagca
    33961 actggaagaa tcagtgtcaa cttttttcaa gtgattttct gattttataa ttcgtcaaat
    34021 tcttttggat caacatcttt gaaacttaag atccacgcat tcatttcatc tgaatcattc
    34081 aagatattga tatcttcacc gattttatca ttaacagaag aaactgtacc cgttagcggg
    34141 ctggcaaatt cattgaccgc tttttccgct tcaacttcga tcagaggttc atcttttttt
    34201 agcgattgtc ctgcttttgg cagattggca aacgtgatgt ctccaagttc ttcttgcgct
    34261 tcatttgtca aaccaacaca gtattctttt ccattaaata agatccaaag gttgtccttt
    34321 ttcttcaaat gcttgcctgc cattttatat cagttctcct tcataaattt tttcattgaa
    34381 tccaattgca acaagctggt cctctcgtaa gatcagtggt cgcttgatca gcataccatc
    34441 tgtcgataag acttggctcg cttttgaagc cgtaaactcg cctacttgat ctttcagtcc
    34501 aagttcccga tatttcatcc cgcttgtgtt gaacaggcga cggattggta cttgactcgt
    34561 ttctagccaa gtctcaatcg ttgaggcctt cggtacttct ttgatcatat caacggtttg
    34621 ataactaacg ccatgctggt ctaaccacgc tttggctttt ttgcatgttg aacatttcgg
    34681 gtaccagtaa aaggtgtaca attttcttac ctccttgaag atcttgcagg tgcttcattt
    34741 tggtatctca tcgataatat caacccgata ccgatcatgt tccctaaaat cgaggatcct
    34801 ccctgactaa tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat atttgctcca
    34861 atattctcaa agacatggaa cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa
    34921 aattcattat tcgtatcgaa acacacccga atcatacgat agattaaaat aaaatacaag
    34981 aaaatgacga atgcaccacc gataaagccg aagttttcac cgataacggt aaaaatcata
    35041 tcggactcac gaacaggcac atagatattg cttacgttga atcctttacc aaacaagcca
    35101 ccggtaccaa ttgccaaaag tccttgggcc ggctgataac tcattcctga cgtatcatgg
    35161 aaagggtcca gccatgaatc aatacgtgca aactgataag atttgaatcc aactttataa
    35221 agcaagtctc ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc aacgactact
    35281 gccaagatta ctacaggtgc gatgatgcgc caagagattc cagacatcag gaaaatcccg
    35341 ccaaagatcg caagaaagac aagcatcgtt ccaaagtcat cttgggccat gatcaatgct
    35401 aaaaccggta ttgtcacgat caagagcttc ccaatcagcc aaaagtccga tttcatcgtc
    35461 cgttctttta cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa gatgtacgca
    35521 atcttcatca actctgctgg ctggaaagta aacgcaccga atcgaaacca gtttttagac
    35581 cctgttgaag cagctaaatt ctgatcatag aatttgagca agagtcccat taccactaac
    35641 cccaaaccat aaaggtaagg agtcagtttc cagatccatt tggaattcaa atgcatgatg
    35701 acaacgattg ctgccgctcc aactaaatac cagacaactt gcatccctac gcctttcaag
    35761 actgctggat tgttcggatc atggctaagt gctacatata atgagagcag accaatcaaa
    35821 cataacagaa agactggtaa aatcacgcca taatcaatac gattatcgtt cgaagctttt
    35881 aatttttttt ccatgtttca tcccttttct taaaaaccat actgctgatt attatagcgg
    35941 tccagttaga taatggaaag tctttccata gacatttggg aaaaattaac tttccgtttg
    36001 tgcagttgct tgagcaaatg cagtacaaaa gcctactgct tatctttagt gtatcataga
    36061 actttttttg atacatgcaa ttatctacca gctgagattc tcctcgctgt gagaagtttc
    36121 tgtgctttca ttgtatcgaa ggatctcttc ggcaatctct agcagctgat cagaaaccag
    36181 attggttcgt tggatgaaat aaaaatatcg atggaaattc aacggagtca ttggcacatt
    36241 ttctggaaca gagcgcttag acaaaatcga gcgaccgatt ccttccttca gcaacgcaac
    36301 aattacatcg ttacttttca cgatcatctt cttatcttgg atattcatct cttcaaaata
    36361 acgctgcgtg taatgaaaaa caccagagtt tgcttctctt acaagccaca atttgctttt
    36421 tgggtcccca gcgatgacta gctggtcttc catcaaagag tatctttgta cgccggaagt
    36481 ctctaatggc ttttcgataa aaccaaaatc ggattcatgt ttcgctactt gttctaatga
    36541 ttcatgagaa ttcgtgattt cgatctcaaa atgcacttta ggaaaccttt tgatcagatg
    36601 tttcatcaac tgaggcaaaa gatagacagc aaaggtgtgg gaagcaacga tccgacatac
    36661 ttcttcttca ttttgttcat tgagcaattt ttgctgagtt tcttcccatt cttctaacaa
    36721 aaccagtgtc cgttcatata ataagtcggc ttgcttagtt ggaagcatct cttttcttcc
    36781 gttacgttga aaaagatcga tattcaattc ccgctccaac tgtttgattt gattcgagac
    36841 tgctggttga gataaaaaca gtacttctgc tgctctggaa aaatttcttg tttcatatac
    36901 tgctcgaaaa gtcgttaata acttaaacat ggcaccactt ccataacttt ttatgataat
    36961 tactataaaa actatttgat ttaaatatac aaaaaaccat ctataataga aatgtacaac
    37021 aaaacagttg ctgatccaac tagtaactta gatgaagaca atcagctgtc tgcttgctcg
    37081 taatcataca ttcctattca ttttacaatg tattcgttca tcttggtact aaaatctatt
    37141 aaataaaaag gagaatatta tatatgaaga actttttgaa aattattccg atcccgatct
    37201 gcgggttgat ccttgggtta gcttcccttg gaaatctgtt taaagactat caccatgttg
    37261 ctttaggcaa tctaactggc ggtatctcta tgattcttat gatattcatt tttgttaaat
    37321 tgtttatctt attcgaacac acgaaacaaa cgttgcatga tccaatcatc gcctccgttt
    37381 caccaacttt tacgatgtca ctcatggtta tttgtactta ttttgtttca tatgaaagta
    37441 ttgcaccaat tgtcaaattc atttggctgt gcgctgttat cttccaagtg atccttgtct
    37501 tttatttcaa ttatcaccat gttatcaaag ctgatctttc aattgaagca atttatccaa
    37561 gctggtttat catttacgtt ggatttgggg tcatcactgt aactgccggg aacttcttcc
    37621 cacttattgg aaaaattttc ttttggattt cattgatctg ctatgcgttc ttattgccga
    37681 tcatcatcaa acgtgtcttt ttcgtaagaa atatggctca tccgacacgt ccgctgatta
    37741 cgatcgttgc agcacctggt tctctttgcc taacaggtta tcttaaaaac tttgctgaac
    37801 ctaattttta tcttgtcgta gcattgttcg ctttatctca agtattgtat ttcgttgtgc
    37861 tgtctatgct tccacgtttg ttcaagcttc gtttttatcc aagctatgca gcctttacat
    37921 tcccgcttgt tatctctgca acagctatat ttactactgt tcattacttc aacagtttag
    37981 gtatctatag tagcgtattg gatttcttaa aagttgcgga atgtctgttt gcaagtgtag
    38041 ttgtcttcta cgttcttgct cattacgtac gtttcttaat taaagaacat agcaaacatg
    38101 gaaaatttgt ttctaaaaaa atcgcttaat aatcgattca ataggttgtg aaaaagctgt
    38161 tctgcatcaa gtaataagaa cggccaaaca aaaacagctg ttcatgtttt tgtactggtt
    38221 gggcttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtc gtaa
//
LOCUS       DACETN010000014        38176 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DACETN010000014 DACETN010000000
VERSION     DACETN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.015"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            308..1192
                     /locus_tag="IXF98_000681"
     CDS             308..1192
                     /locus_tag="IXF98_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555014.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1401..1979
                     /gene="pgsA"
                     /locus_tag="IXF98_000682"
     CDS             1401..1979
                     /gene="pgsA"
                     /locus_tag="IXF98_000682"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1555015.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2219..3460
                     /locus_tag="IXF98_000683"
     CDS             2219..3460
                     /locus_tag="IXF98_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1555016.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3569..4618
                     /gene="recA"
                     /locus_tag="IXF98_000684"
     CDS             3569..4618
                     /gene="recA"
                     /locus_tag="IXF98_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1555017.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5029..6585
                     /gene="rny"
                     /locus_tag="IXF98_000685"
     CDS             5029..6585
                     /gene="rny"
                     /locus_tag="IXF98_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1555018.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IXF98_000686"
     CDS             complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IXF98_000686"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1555019.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7611..10184
                     /gene="mprF"
                     /locus_tag="IXF98_000687"
     CDS             7611..10184
                     /gene="mprF"
                     /locus_tag="IXF98_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1555020.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10338..11189
                     /locus_tag="IXF98_000688"
     CDS             10338..11189
                     /locus_tag="IXF98_000688"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1555021.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11434..12372
                     /locus_tag="IXF98_000689"
     CDS             11434..12372
                     /locus_tag="IXF98_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555022.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12395..13075
                     /locus_tag="IXF98_000690"
     CDS             12395..13075
                     /locus_tag="IXF98_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555023.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13048..13872
                     /locus_tag="IXF98_000691"
     CDS             13048..13872
                     /locus_tag="IXF98_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1555024.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14039..14857
                     /gene="purR"
                     /locus_tag="IXF98_000692"
     CDS             14039..14857
                     /gene="purR"
                     /locus_tag="IXF98_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1555025.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15360..16733
                     /gene="glmU"
                     /locus_tag="IXF98_000693"
     CDS             15360..16733
                     /gene="glmU"
                     /locus_tag="IXF98_000693"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1555026.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16827..17798
                     /locus_tag="IXF98_000694"
     CDS             16827..17798
                     /locus_tag="IXF98_000694"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1555027.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17804..17906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17972..18541
                     /gene="thiT"
                     /locus_tag="IXF98_000695"
     CDS             17972..18541
                     /gene="thiT"
                     /locus_tag="IXF98_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1555028.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18622..19395)
                     /locus_tag="IXF98_000696"
     CDS             complement(18622..19395)
                     /locus_tag="IXF98_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555029.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19661..21361
                     /locus_tag="IXF98_000697"
     CDS             19661..21361
                     /locus_tag="IXF98_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1555030.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21331..21549
                     /locus_tag="IXF98_000698"
     CDS             21331..21549
                     /locus_tag="IXF98_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555031.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21569..22459
                     /locus_tag="IXF98_000699"
     CDS             21569..22459
                     /locus_tag="IXF98_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1555032.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22477..22761
                     /locus_tag="IXF98_000700"
     CDS             22477..22761
                     /locus_tag="IXF98_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555033.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23248..23862
                     /gene="gmk"
                     /locus_tag="IXF98_000701"
     CDS             23248..23862
                     /gene="gmk"
                     /locus_tag="IXF98_000701"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1555034.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23867..24172
                     /locus_tag="IXF98_000702"
     CDS             23867..24172
                     /locus_tag="IXF98_000702"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1555035.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24425..26833
                     /gene="priA"
                     /locus_tag="IXF98_000703"
     CDS             24425..26833
                     /gene="priA"
                     /locus_tag="IXF98_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1555036.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26848..27339
                     /gene="def"
                     /locus_tag="IXF98_000704"
     CDS             26848..27339
                     /gene="def"
                     /locus_tag="IXF98_000704"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1555037.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27332..28270
                     /locus_tag="IXF98_000705"
     CDS             27332..28270
                     /locus_tag="IXF98_000705"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1555038.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28270..29628
                     /gene="rsmB"
                     /locus_tag="IXF98_000706"
     CDS             28270..29628
                     /gene="rsmB"
                     /locus_tag="IXF98_000706"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1555039.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29641..30381
                     /locus_tag="IXF98_000707"
     CDS             29641..30381
                     /locus_tag="IXF98_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1555040.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30378..32447
                     /gene="pknB"
                     /locus_tag="IXF98_000708"
     CDS             30378..32447
                     /gene="pknB"
                     /locus_tag="IXF98_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1555041.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32612..33511
                     /gene="rsgA"
                     /locus_tag="IXF98_000709"
     CDS             32612..33511
                     /gene="rsgA"
                     /locus_tag="IXF98_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1555042.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33524..34174
                     /locus_tag="IXF98_000710"
     CDS             33524..34174
                     /locus_tag="IXF98_000710"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1555043.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34175..34813
                     /locus_tag="IXF98_000711"
     CDS             34175..34813
                     /locus_tag="IXF98_000711"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1555044.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            34968..35822
                     /gene="mreC"
                     /locus_tag="IXF98_000712"
     CDS             34968..35822
                     /gene="mreC"
                     /locus_tag="IXF98_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1555045.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35826..36335
                     /gene="mreD"
                     /locus_tag="IXF98_000713"
     CDS             35826..36335
                     /gene="mreD"
                     /locus_tag="IXF98_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1555046.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36590..38128
                     /locus_tag="IXF98_000714"
     CDS             36590..38128
                     /locus_tag="IXF98_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555047.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        12553 a         6828 c         8305 g        10490 t
ORIGIN      
        1 acggaaaaat atctccttat attttgccgg tttttgagct aataccgagg aacagctgat
       61 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca
      121 agttcaaaaa acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc
      181 aggagaacaa tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca
      241 aacataacgt ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga
      301 tgaacgagtg gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa
      361 aatatcttta gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat
      421 cgacaatgat gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca
      481 atacgctgaa gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag
      541 caaattgaca ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa
      601 agcaatgcat caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt
      661 attaggattg gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg
      721 tcaagctgat ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga
      781 ggagtcctct tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac
      841 aacaaaatca agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc
      901 catcgcagta aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa
      961 tcgtgtatgg ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc
     1021 aaatgaaaca cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg
     1081 tgcagcaagc aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga
     1141 aaacaatcag agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa
     1201 attagtttac gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg
     1261 tcgcggtcct tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata
     1321 aattcatttg gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag
     1381 ggaagaaagg agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta
     1441 tgatcccgat tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag
     1501 gagacaccac actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta
     1561 tcacagactg gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca
     1621 agtttgcgga cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg
     1681 accaaggaaa ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca
     1741 caggcttgcg tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa
     1801 aagtcaagac ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat
     1861 tcagcctgat cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta
     1921 tctattcagg tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag
     1981 tatcgtttag tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt
     2041 caaaaaacag cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga
     2101 gaacactgtt tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct
     2161 ctttttaaat aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat
     2221 gaaagcagaa ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa
     2281 tgccactttc ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt
     2341 tgtaggagat aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga
     2401 actcatactc ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac
     2461 tgcggctcac ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc
     2521 atattttgaa acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat
     2581 cgaaggcggg gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac
     2641 agatacacat gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt
     2701 ggaacaagta cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa
     2761 agttttgcgc ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat
     2821 tgagacacag atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg
     2881 actgacagtg aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga
     2941 aaaaatccaa gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc
     3001 aaaagtcgta gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt
     3061 gacagccggc gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc
     3121 aggcggattc gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga
     3181 gctattagca gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc
     3241 acgtgagata gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac
     3301 gttagaaggt caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt
     3361 ttcggaaaaa tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca
     3421 aggatttgat cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg
     3481 gttttttctt gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg
     3541 atttgtaaat ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc
     3601 tgcactgaaa aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa
     3661 aatcgaccaa caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg
     3721 tgtcggcggt tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa
     3781 aacaacagtt gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt
     3841 cattgatgct gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga
     3901 tgaactactt ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt
     3961 agtatcaagt ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg
     4021 agcagaaatc gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc
     4081 tcaagcattg cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat
     4141 caaccaaatc cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg
     4201 acgcgcattg aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa
     4261 acaaggtaca gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc
     4321 acctcctttc aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg
     4381 agaacttcta gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc
     4441 ttataaagaa gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca
     4501 tcctgaaatg atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga
     4561 agtcgctgtc ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa
     4621 attatgataa acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt
     4681 tttattgaat aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa
     4741 tatctgttca tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct
     4801 atatgaaact agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt
     4861 ttctttgttt atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg
     4921 ttttttgtac acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga
     4981 agctgtatag atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac
     5041 attctcctcg ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag
     5101 tcacgtcacg agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt
     5161 gcaaaaaaag aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat
     5221 cagaaatacc gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct
     5281 caagagaatc gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa
     5341 aaacgcgaga actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt
     5401 gaagagcgag aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa
     5461 attgctggtt tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag
     5521 ctgaatcatg agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca
     5581 gatcgcaaag cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt
     5641 tcagaaacga ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt
     5701 ggacgagaag gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc
     5761 gatgatacac cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca
     5821 cggatgacct tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa
     5881 atggtcgaga aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca
     5941 gcgtttgaag tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg
     6001 cgattcagaa ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta
     6061 gctggtatct tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg
     6121 ctccatgata ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt
     6181 gcggaattag ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat
     6241 catggagatg tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc
     6301 tcagcagcaa gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga
     6361 agtctggaaa atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct
     6421 ggacgcgaag ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt
     6481 ttagtacgtg atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa
     6541 gtaatcgtta ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag
     6601 aagagccgcg gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta
     6661 tcattatttt ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat
     6721 gattgctggg atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc
     6781 aggagaagaa acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg
     6841 acaagaaccc ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt
     6901 cagtacacca gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc
     6961 agcaaggcca atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc
     7021 ttcaaaaatc atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat
     7081 cgcagaaacg cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc
     7141 ctgaggatct tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat
     7201 gaataagact ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg
     7261 tttcacggct aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc
     7321 atggacgcca gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa
     7381 cacctcttct ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat
     7441 attccctgtg ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct
     7501 ttctttttct atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa
     7561 gataacattg gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata
     7621 aacgatactt gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt
     7681 tcggctccgt gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt
     7741 ggcaagatat ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc
     7801 tggctggact gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct
     7861 tagaaaaaca aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa
     7921 caaacacgat caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta
     7981 gtttttatgg aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac
     8041 tatttatgct gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta
     8101 ttcagtcaga aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag
     8161 caccagtcat tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc
     8221 caaaaggctt gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg
     8281 tatttttgat catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta
     8341 tgttcatgat tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt
     8401 ttgacgtttt gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag
     8461 tttggctatt atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat
     8521 tgttcatcac acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt
     8581 ttttccaaaa ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga
     8641 tggtacttct atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat
     8701 tgccgttctc attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac
     8761 tcggccttgc tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt
     8821 tactatcttt tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg
     8881 tatatgtctt gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat
     8941 tcgtttattc ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg
     9001 tttacgcaat tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc
     9061 attttatttt gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct
     9121 cattgctagc tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca
     9181 gtgagtacga aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa
     9241 gccatcagct tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc
     9301 aagtcgtttt tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg
     9361 gcaatcctaa aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag
     9421 gctatcagct tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg
     9481 gttttcactt cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt
     9541 ctcggctgag tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt
     9601 ggtacgatca actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc
     9661 ttcaaggtgc acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat
     9721 ctagcggtgt cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc
     9781 cgatgacgaa gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg
     9841 aattagctga ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg
     9901 aagccaatct aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc
     9961 aagaaaaagt catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg
    10021 cttatgaaaa gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga
    10081 aaggaagtaa tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag
    10141 gaaatgttcc gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg
    10201 tttggattat gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact
    10261 aggcagacat aagctatact tttatttttc cctatgatag aatacttgcg acttagactt
    10321 ttcgaatggg tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac
    10381 tggatgtatt atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg
    10441 tagacttggc agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa
    10501 caaataaagc atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc
    10561 taaaaaataa atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag
    10621 tagcagctgg attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc
    10681 aattatggaa tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta
    10741 cagatgttcc ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa
    10801 tccaagtatt accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg
    10861 tctctacacg tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc
    10921 acggacttgc tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa
    10981 acagtttaga agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta
    11041 tgatcaagta cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct
    11101 tgtgtcaaca ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg
    11161 aagtttattt agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac
    11221 tgaggaatcc taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag
    11281 cagcaactca tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat
    11341 ggatttctat tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa
    11401 aattccgatt tgttcattgg aaggagcata taaatgaaaa aatatctttt gacagctacg
    11461 gtaatgatag gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa
    11521 aaagaggatt tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc
    11581 gtaggggatg aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc
    11641 gaaccaagtg caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt
    11701 tcagacatgg aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg
    11761 atgatcgaag cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa
    11821 gaatcagaag aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg
    11881 gtattagcta taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat
    11941 aaaaaggaaa cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat
    12001 caaaaatact ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct
    12061 gcttttgctt atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca
    12121 ccagatcagg aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac
    12181 aacatcaaag tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg
    12241 aatgaaacag gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa
    12301 atcaaagcag gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa
    12361 agtatcaatt aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt
    12421 gaccttttac tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg
    12481 tgaatttgtc attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc
    12541 gttaggatta ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg
    12601 aaaaaaatta agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc
    12661 tagtacggtg atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg
    12721 tctctctgaa aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga
    12781 tatgcgaaat cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc
    12841 gcgtattttt gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga
    12901 tgaagaatcc agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa
    12961 atcagttcta atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat
    13021 ccgactggtg agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga
    13081 gcgtttcttg ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg
    13141 atcagaagac aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg
    13201 ttaggattct ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca
    13261 gctgtgatat tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc
    13321 attttaatgg ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat
    13381 tcagcaacaa gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa
    13441 gtcatctttt taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg
    13501 ccgatgtatg ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat
    13561 ttgatgtcga tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt
    13621 gccggtgcac ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta
    13681 ggcaaaagct tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg
    13741 tcaggactaa ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc
    13801 tttattgctt tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga
    13861 aaaaataact aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct
    13921 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
    13981 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
    14041 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
    14101 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
    14161 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
    14221 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
    14281 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
    14341 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
    14401 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
    14461 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
    14521 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
    14581 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
    14641 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
    14701 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
    14761 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
    14821 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
    14881 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
    14941 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
    15001 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
    15061 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
    15121 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
    15181 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
    15241 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
    15301 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
    15361 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
    15421 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
    15481 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
    15541 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
    15601 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
    15661 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
    15721 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
    15781 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
    15841 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
    15901 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
    15961 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
    16021 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
    16081 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
    16141 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
    16201 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
    16261 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
    16321 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
    16381 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
    16441 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
    16501 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
    16561 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
    16621 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
    16681 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
    16741 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
    16801 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
    16861 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
    16921 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
    16981 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
    17041 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
    17101 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
    17161 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
    17221 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
    17281 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
    17341 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
    17401 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
    17461 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
    17521 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
    17581 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
    17641 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
    17701 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
    17761 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
    17821 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
    17881 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
    17941 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
    18001 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
    18061 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
    18121 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
    18181 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
    18241 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
    18301 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
    18361 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt
    18421 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
    18481 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
    18541 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
    18601 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
    18661 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
    18721 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
    18781 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
    18841 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
    18901 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
    18961 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
    19021 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
    19081 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
    19141 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
    19201 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
    19261 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
    19321 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
    19381 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
    19441 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
    19501 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga
    19561 tgttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    19621 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    19681 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    19741 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    19801 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    19861 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    19921 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    19981 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    20041 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg
    20101 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    20161 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    20221 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    20281 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    20341 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    20401 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    20461 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    20521 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    20581 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    20641 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    20701 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    20761 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    20821 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    20881 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    20941 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    21001 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    21061 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    21121 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    21181 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    21241 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    21301 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    21361 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    21421 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    21481 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    21541 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    21601 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    21661 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    21721 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    21781 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    21841 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    21901 ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    21961 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    22021 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    22081 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    22141 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    22201 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    22261 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    22321 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    22381 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    22441 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    22501 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    22561 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    22621 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    22681 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    22741 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa
    22801 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    22861 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    22921 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    22981 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    23041 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    23101 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    23161 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    23221 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    23281 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    23341 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    23401 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    23461 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    23521 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    23581 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    23641 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa
    23701 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    23761 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    23821 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    23881 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    23941 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    24001 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    24061 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    24121 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    24181 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    24241 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    24301 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    24361 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    24421 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    24481 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    24541 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    24601 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    24661 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    24721 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    24781 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    24841 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    24901 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    24961 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    25021 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    25081 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    25141 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    25201 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    25261 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    25321 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    25381 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    25441 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    25501 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    25561 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    25621 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    25681 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    25741 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    25801 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    25861 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    25921 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    25981 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    26041 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    26101 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    26161 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    26221 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    26281 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    26341 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    26401 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    26461 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    26521 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    26581 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    26641 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    26701 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    26761 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    26821 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    26881 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    26941 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    27001 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    27061 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    27121 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    27181 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    27241 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    27301 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    27361 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    27421 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    27481 agaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    27541 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    27601 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    27661 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    27721 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    27781 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    27841 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    27901 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    27961 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    28021 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    28081 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    28141 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    28201 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    28261 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    28321 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    28381 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    28441 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    28501 aaagtggatg actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta
    28561 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    28621 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    28681 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    28741 ctatggatga ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    28801 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    28861 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    28921 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    28981 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    29041 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    29101 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    29161 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    29221 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    29281 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    29341 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    29401 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    29461 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    29521 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    29581 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    29641 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    29701 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    29761 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    29821 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    29881 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    29941 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    30001 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    30061 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    30121 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    30181 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    30241 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    30301 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    30361 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    30421 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    30481 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    30541 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    30601 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    30661 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    30721 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    30781 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    30841 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    30901 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    30961 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    31021 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    31081 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    31141 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    31201 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    31261 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    31321 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    31381 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    31441 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    31501 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    31561 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    31621 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    31681 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    31741 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    31801 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    31861 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    31921 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    31981 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    32041 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    32101 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    32161 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    32221 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    32281 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    32341 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    32401 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    32461 tatatcagaa gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct
    32521 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    32581 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    32641 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    32701 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    32761 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    32821 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    32881 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    32941 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    33001 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    33061 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    33121 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    33181 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    33241 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    33301 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    33361 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    33421 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    33481 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    33541 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    33601 cgcagattac atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg
    33661 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    33721 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    33781 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    33841 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    33901 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    33961 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    34021 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    34081 tgcaggggcg gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg
    34141 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    34201 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    34261 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    34321 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    34381 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    34441 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    34501 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    34561 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    34621 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    34681 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    34741 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    34801 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    34861 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    34921 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    34981 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    35041 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    35101 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    35161 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    35221 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    35281 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    35341 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    35401 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    35461 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    35521 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    35581 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    35641 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    35701 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    35761 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    35821 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    35881 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    35941 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    36001 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    36061 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    36121 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    36181 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    36241 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    36301 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    36361 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    36421 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    36481 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    36541 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    36601 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    36661 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    36721 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    36781 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    36841 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    36901 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    36961 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    37021 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    37081 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    37141 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    37201 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    37261 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    37321 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    37381 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    37441 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    37501 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    37561 aacagtgcct gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac
    37621 agaagaatca acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg
    37681 aaacggcaca ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa
    37741 acctgtaaca cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga
    37801 agcttacaaa tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga
    37861 ctgctcaggg ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg
    37921 gacagttcct caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga
    37981 cttgttattc tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg
    38041 acaatatatc catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt
    38101 tgcccctgac tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct
    38161 ggactaactc tttttt
//
LOCUS       DACETN010000015        38110 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DACETN010000015 DACETN010000000
VERSION     DACETN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.016"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(451..1761)
                     /locus_tag="IXF98_000715"
     CDS             complement(451..1761)
                     /locus_tag="IXF98_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1555048.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IXF98_000716"
     CDS             complement(1773..3191)
                     /locus_tag="IXF98_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1555049.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IXF98_000717"
     CDS             complement(3431..4999)
                     /locus_tag="IXF98_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1555050.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IXF98_000718"
     CDS             complement(5003..5413)
                     /locus_tag="IXF98_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1555051.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IXF98_000719"
     CDS             complement(5410..6333)
                     /locus_tag="IXF98_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1555052.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IXF98_000720"
     CDS             complement(6330..7208)
                     /locus_tag="IXF98_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1555053.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IXF98_000721"
     CDS             complement(7195..8532)
                     /locus_tag="IXF98_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1555054.1"
                     /translation="MYDILTFGASIAELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IXF98_000722"
     CDS             complement(8535..9638)
                     /locus_tag="IXF98_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1555055.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IXF98_000723"
     CDS             complement(9712..11220)
                     /locus_tag="IXF98_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1555056.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IXF98_000724"
     CDS             complement(11186..11878)
                     /locus_tag="IXF98_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1555057.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IXF98_000725"
     CDS             complement(11900..12874)
                     /locus_tag="IXF98_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555058.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IXF98_000726"
     CDS             13174..14046
                     /locus_tag="IXF98_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555059.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IXF98_000727"
     CDS             complement(14504..16702)
                     /locus_tag="IXF98_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555060.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IXF98_000728"
     CDS             16934..17926
                     /locus_tag="IXF98_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1555061.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IXF98_000729"
     CDS             17986..19407
                     /locus_tag="IXF98_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1555062.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IXF98_000730"
     CDS             19400..20899
                     /locus_tag="IXF98_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1555063.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IXF98_000731"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IXF98_000731"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1555064.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IXF98_000732"
     CDS             22491..23174
                     /locus_tag="IXF98_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1555065.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IXF98_000733"
     CDS             23176..24012
                     /locus_tag="IXF98_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1555066.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IXF98_000734"
     CDS             24023..25777
                     /locus_tag="IXF98_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555067.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IXF98_000735"
     CDS             complement(26053..26394)
                     /locus_tag="IXF98_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1555068.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IXF98_000736"
     CDS             complement(26462..28633)
                     /locus_tag="IXF98_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1555069.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IXF98_000737"
     CDS             28844..29701
                     /locus_tag="IXF98_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1555070.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IXF98_000738"
     CDS             complement(29772..30629)
                     /locus_tag="IXF98_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1555071.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IXF98_000739"
     CDS             complement(30614..33316)
                     /locus_tag="IXF98_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1555072.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IXF98_000740"
     CDS             complement(33329..34618)
                     /locus_tag="IXF98_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1555073.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IXF98_000741"
     CDS             34768..35814
                     /locus_tag="IXF98_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1555074.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IXF98_000742"
     CDS             35816..37969
                     /locus_tag="IXF98_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1555075.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11245 a         7840 c         6771 g        12254 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcagca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca
//
LOCUS       DACETN010000016        37588 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DACETN010000016 DACETN010000000
VERSION     DACETN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.017"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(136..2289)
                     /locus_tag="IXF98_000743"
     CDS             complement(136..2289)
                     /locus_tag="IXF98_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1555076.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IXF98_000744"
     CDS             complement(2583..3431)
                     /locus_tag="IXF98_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1555077.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXF98_000745"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXF98_000745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1555078.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IXF98_000746"
     CDS             complement(5932..6111)
                     /locus_tag="IXF98_000746"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1555079.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXF98_000747"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXF98_000747"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1555080.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..10538
                     /locus_tag="IXF98_000748"
     CDS             8259..10538
                     /locus_tag="IXF98_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1555081.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10631..11278)
                     /locus_tag="IXF98_000749"
     CDS             complement(10631..11278)
                     /locus_tag="IXF98_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555082.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11457..12176)
                     /locus_tag="IXF98_000750"
     CDS             complement(11457..12176)
                     /locus_tag="IXF98_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1555083.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12307..13458)
                     /locus_tag="IXF98_000751"
     CDS             complement(12307..13458)
                     /locus_tag="IXF98_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1555084.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13675..14415)
                     /locus_tag="IXF98_000752"
     CDS             complement(13675..14415)
                     /locus_tag="IXF98_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1555085.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXF98_000753"
     CDS             complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXF98_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1555086.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14799..15392)
                     /locus_tag="IXF98_000754"
     CDS             complement(14799..15392)
                     /locus_tag="IXF98_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1555087.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15376..16023)
                     /locus_tag="IXF98_000755"
     CDS             complement(15376..16023)
                     /locus_tag="IXF98_000755"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1555088.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXF98_000756"
     CDS             complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXF98_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1555089.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXF98_000757"
     CDS             complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXF98_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1555090.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17469..17996)
                     /locus_tag="IXF98_000758"
     CDS             complement(17469..17996)
                     /locus_tag="IXF98_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1555091.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18176..18961)
                     /locus_tag="IXF98_000759"
     CDS             complement(18176..18961)
                     /locus_tag="IXF98_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1555092.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18954..19823)
                     /locus_tag="IXF98_000760"
     CDS             complement(18954..19823)
                     /locus_tag="IXF98_000760"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1555093.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19824..21203)
                     /locus_tag="IXF98_000761"
     CDS             complement(19824..21203)
                     /locus_tag="IXF98_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1555094.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXF98_000762"
     CDS             complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXF98_000762"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1555095.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXF98_000763"
     CDS             complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXF98_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1555096.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXF98_000764"
     CDS             complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXF98_000764"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1555097.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXF98_000765"
     CDS             complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXF98_000765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1555098.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXF98_000766"
     CDS             complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXF98_000766"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1555099.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25161..25385)
                     /locus_tag="IXF98_000767"
     CDS             complement(25161..25385)
                     /locus_tag="IXF98_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1555100.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25438..26400)
                     /locus_tag="IXF98_000768"
     CDS             complement(25438..26400)
                     /locus_tag="IXF98_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1555101.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26410..26850)
                     /locus_tag="IXF98_000769"
     CDS             complement(26410..26850)
                     /locus_tag="IXF98_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1555102.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27156..28121
                     /locus_tag="IXF98_000770"
     CDS             27156..28121
                     /locus_tag="IXF98_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1555103.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28178..28408)
                     /locus_tag="IXF98_000771"
     CDS             complement(28178..28408)
                     /locus_tag="IXF98_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1555104.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28494..29381)
                     /locus_tag="IXF98_000772"
     CDS             complement(28494..29381)
                     /locus_tag="IXF98_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1555105.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29470..30693)
                     /locus_tag="IXF98_000773"
     CDS             complement(29470..30693)
                     /locus_tag="IXF98_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1555106.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30710..31789)
                     /locus_tag="IXF98_000774"
     CDS             complement(30710..31789)
                     /locus_tag="IXF98_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1555107.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IXF98_000775"
     CDS             complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IXF98_000775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1555108.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33647..33913)
                     /locus_tag="IXF98_000776"
     CDS             complement(33647..33913)
                     /locus_tag="IXF98_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1555109.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34392..36626
                     /locus_tag="IXF98_000777"
     CDS             34392..36626
                     /locus_tag="IXF98_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1555110.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36774..37094
                     /locus_tag="IXF98_000778"
     CDS             36774..37094
                     /locus_tag="IXF98_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1555111.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37299..37523)
                     /locus_tag="IXF98_000779"
     CDS             complement(37299..37523)
                     /locus_tag="IXF98_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1555112.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT        10546 a         8126 c         6531 g        12385 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
    10561 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
    10621 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
    10681 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
    10741 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
    10801 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
    10861 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
    10921 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
    10981 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
    11041 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
    11101 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
    11161 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
    11221 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
    11281 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
    11341 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
    11401 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
    11461 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
    11521 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
    11581 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
    11641 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
    11701 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
    11761 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
    11821 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
    11881 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
    11941 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
    12001 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
    12061 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
    12121 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
    12181 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
    12241 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
    12301 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
    12361 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
    12421 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
    12481 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    12541 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    12601 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    12661 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    12721 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    12781 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    12841 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    12901 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    12961 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    13021 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    13081 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    13141 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    13201 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    13261 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    13321 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    13381 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    13441 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    13501 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    13561 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    13621 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    13681 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    13741 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    13801 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    13861 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    13921 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    13981 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    14041 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    14101 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    14161 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    14221 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    14281 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    14341 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    14401 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    14461 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    14521 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    14581 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    14641 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    14701 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    14761 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    14821 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    14881 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    14941 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    15001 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    15061 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    15121 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    15181 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    15241 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    15301 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    15361 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    15421 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    15481 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    15541 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    15601 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    15661 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    15721 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    15781 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    15841 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    15901 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    15961 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    16021 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    16081 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    16141 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    16201 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    16261 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    16321 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    16381 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    16441 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    16501 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    16561 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    16621 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    16681 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    16741 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    16801 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    16861 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    16921 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    16981 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    17041 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    17101 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    17161 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    17221 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    17281 caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa
    17341 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    17401 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    17461 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    17521 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    17581 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    17641 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    17701 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    17761 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    17821 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    17881 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    17941 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    18001 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    18061 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    18121 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    18181 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    18241 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    18301 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    18361 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    18421 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    18481 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    18541 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    18601 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    18661 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    18721 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    18781 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    18841 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    18901 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    18961 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    19021 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    19081 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    19141 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    19201 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    19261 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    19321 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    19381 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    19441 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    19501 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    19561 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    19621 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    19681 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    19741 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    19801 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    19861 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    19921 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    19981 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    20041 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    20101 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    20161 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    20221 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    20281 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    20341 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    20401 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    20461 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    20521 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    20581 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    20641 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    20701 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    20761 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    20821 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    20881 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    20941 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    21001 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    21061 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    21121 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    21181 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    21241 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    21301 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    21361 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    21421 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    21481 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    21541 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    21601 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    21661 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    21721 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    21781 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    21841 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    21901 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac
    21961 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    22021 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    22081 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    22141 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    22201 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    22261 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    22321 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    22381 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    22441 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    22501 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    22561 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    22621 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    22681 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    22741 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    22801 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    22861 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    22921 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    22981 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    23041 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    23101 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    23161 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    23221 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    23281 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    23341 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    23401 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    23461 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    23521 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    23581 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    23641 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    23701 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    23761 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    23821 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    23881 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    23941 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    24001 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    24061 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    24121 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    24181 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    24241 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    24301 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    24361 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    24421 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    24481 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    24541 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    24601 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    24661 tcactgcaac cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt
    24721 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    24781 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    24841 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    24901 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    24961 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    25021 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    25081 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    25141 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    25201 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    25261 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    25321 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    25381 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    25441 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    25501 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    25561 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    25621 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    25681 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    25741 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    25801 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    25861 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    25921 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    25981 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    26041 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    26101 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    26161 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    26221 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    26281 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    26341 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    26401 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    26461 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    26521 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    26581 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    26641 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    26701 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    26761 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    26821 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    26881 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    26941 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    27001 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    27061 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    27121 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    27181 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    27241 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    27301 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    27361 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    27421 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    27481 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    27541 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    27601 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    27661 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    27721 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    27781 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    27841 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    27901 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    27961 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    28021 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    28081 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    28141 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    28201 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    28261 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    28321 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    28381 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    28441 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    28501 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    28561 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    28621 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    28681 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    28741 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    28801 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    28861 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    28921 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    28981 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    29041 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    29101 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    29161 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    29221 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    29281 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    29341 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    29401 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    29461 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    29521 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    29581 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    29641 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    29701 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    29761 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    29821 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    29881 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    29941 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    30001 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    30061 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    30121 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    30181 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    30241 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    30301 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    30361 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    30421 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    30481 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    30541 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    30601 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    30661 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    30721 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    30781 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    30841 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    30901 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    30961 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    31021 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    31081 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    31141 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    31201 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    31261 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    31321 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    31381 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    31441 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga
    31501 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    31561 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    31621 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    31681 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    31741 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    31801 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    31861 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
    31921 tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat
    31981 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt
    32041 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
    32101 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
    32161 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
    32221 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
    32281 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
    32341 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
    32401 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
    32461 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
    32521 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
    32581 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
    32641 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
    32701 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
    32761 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
    32821 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
    32881 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
    32941 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
    33001 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
    33061 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
    33121 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
    33181 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
    33241 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
    33301 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
    33361 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
    33421 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
    33481 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
    33541 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
    33601 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
    33661 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
    33721 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
    33781 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
    33841 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
    33901 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
    33961 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
    34021 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
    34081 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
    34141 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
    34201 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
    34261 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
    34321 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
    34381 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
    34441 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
    34501 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
    34561 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
    34621 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
    34681 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
    34741 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
    34801 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
    34861 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
    34921 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
    34981 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
    35041 tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg
    35101 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
    35161 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
    35221 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
    35281 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
    35341 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
    35401 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
    35461 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
    35521 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
    35581 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
    35641 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
    35701 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
    35761 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
    35821 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
    35881 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
    35941 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
    36001 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
    36061 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
    36121 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
    36181 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
    36241 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
    36301 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
    36361 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
    36421 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
    36481 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
    36541 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
    36601 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
    36661 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
    36721 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
    36781 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
    36841 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg
    36901 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
    36961 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
    37021 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    37081 cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag
    37141 aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg
    37201 gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc
    37261 ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt
    37321 ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt
    37381 tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct
    37441 tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca
    37501 cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa
    37561 aataattttt atattttttt gattagac
//
LOCUS       DACETN010000017        37402 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DACETN010000017 DACETN010000000
VERSION     DACETN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.018"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            221..1084
                     /locus_tag="IXF98_000780"
     CDS             221..1084
                     /locus_tag="IXF98_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1555113.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1213..2835)
                     /locus_tag="IXF98_000781"
     CDS             complement(1213..2835)
                     /locus_tag="IXF98_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1555114.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3084..3605
                     /locus_tag="IXF98_000782"
     CDS             3084..3605
                     /locus_tag="IXF98_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555115.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3791..5521)
                     /gene="spxB"
                     /locus_tag="IXF98_000783"
     CDS             complement(3791..5521)
                     /gene="spxB"
                     /locus_tag="IXF98_000783"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1555116.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5686..6504
                     /locus_tag="IXF98_000784"
     CDS             5686..6504
                     /locus_tag="IXF98_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1555117.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6607..6804)
                     /locus_tag="IXF98_000785"
     CDS             complement(6607..6804)
                     /locus_tag="IXF98_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555118.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6973..7551
                     /locus_tag="IXF98_000786"
     CDS             6973..7551
                     /locus_tag="IXF98_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1555119.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8596..9099)
                     /locus_tag="IXF98_000787"
     CDS             complement(8596..9099)
                     /locus_tag="IXF98_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1555120.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9457..10482
                     /locus_tag="IXF98_000788"
     CDS             9457..10482
                     /locus_tag="IXF98_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1555121.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10805..11683
                     /locus_tag="IXF98_000789"
     CDS             10805..11683
                     /locus_tag="IXF98_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555122.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12159..12275
                     /locus_tag="IXF98_000790"
     CDS             12159..12275
                     /locus_tag="IXF98_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1555123.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13203..13541
                     /locus_tag="IXF98_000791"
     CDS             13203..13541
                     /locus_tag="IXF98_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555124.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13635..13865
                     /locus_tag="IXF98_000792"
     CDS             13635..13865
                     /locus_tag="IXF98_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555125.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14265..14636
                     /locus_tag="IXF98_000793"
     CDS             14265..14636
                     /locus_tag="IXF98_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1555126.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14910..15602)
                     /locus_tag="IXF98_000794"
     CDS             complement(14910..15602)
                     /locus_tag="IXF98_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1555127.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15605..16390)
                     /locus_tag="IXF98_000795"
     CDS             complement(15605..16390)
                     /locus_tag="IXF98_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1555128.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16627..18027
                     /gene="lacG"
                     /locus_tag="IXF98_000796"
     CDS             16627..18027
                     /gene="lacG"
                     /locus_tag="IXF98_000796"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1555129.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18052..18369
                     /locus_tag="IXF98_000797"
     CDS             18052..18369
                     /locus_tag="IXF98_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1555130.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18382..20055
                     /locus_tag="IXF98_000798"
     CDS             18382..20055
                     /locus_tag="IXF98_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1555131.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20069..21700
                     /locus_tag="IXF98_000799"
     CDS             20069..21700
                     /locus_tag="IXF98_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1555132.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22191..23756)
                     /gene="guaA"
                     /locus_tag="IXF98_000800"
     CDS             complement(22191..23756)
                     /gene="guaA"
                     /locus_tag="IXF98_000800"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1555133.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23815..24738)
                     /locus_tag="IXF98_000801"
     CDS             complement(23815..24738)
                     /locus_tag="IXF98_000801"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1555134.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24934..25539
                     /locus_tag="IXF98_000802"
     CDS             24934..25539
                     /locus_tag="IXF98_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1555135.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25847..26863
                     /locus_tag="IXF98_000803"
     CDS             25847..26863
                     /locus_tag="IXF98_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1555136.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27004..28305
                     /locus_tag="IXF98_000804"
     CDS             27004..28305
                     /locus_tag="IXF98_000804"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1555137.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28351..29013
                     /gene="deoC"
                     /locus_tag="IXF98_000805"
     CDS             28351..29013
                     /gene="deoC"
                     /locus_tag="IXF98_000805"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1555138.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29034..29432
                     /locus_tag="IXF98_000806"
     CDS             29034..29432
                     /locus_tag="IXF98_000806"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1555139.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29498..30580
                     /locus_tag="IXF98_000807"
     CDS             29498..30580
                     /locus_tag="IXF98_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1555140.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30792..32354
                     /locus_tag="IXF98_000808"
     CDS             30792..32354
                     /locus_tag="IXF98_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555141.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32347..33480
                     /locus_tag="IXF98_000809"
     CDS             32347..33480
                     /locus_tag="IXF98_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555142.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33480..34436
                     /locus_tag="IXF98_000810"
     CDS             33480..34436
                     /locus_tag="IXF98_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555143.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34535..35701
                     /gene="deoB"
                     /locus_tag="IXF98_000811"
     CDS             34535..35701
                     /gene="deoB"
                     /locus_tag="IXF98_000811"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1555144.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35725..36543
                     /locus_tag="IXF98_000812"
     CDS             35725..36543
                     /locus_tag="IXF98_000812"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1555145.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36560..37267
                     /gene="deoD"
                     /locus_tag="IXF98_000813"
     CDS             36560..37267
                     /gene="deoD"
                     /locus_tag="IXF98_000813"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1555146.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11665 a         6534 c         7907 g        11296 t
ORIGIN      
        1 gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaatttg agaagcaatt
       61 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct cttattttga
      121 ttagaacttg cgaaaataaa ccggttaaag tgtgtctagc gcaagagatt tgctataata
      181 gacgataagg gtaaaatgta acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga
      241 ttcttgttgt gatctgccat accatgtatt agcaaaagaa aaagttgatt ttttatcaat
      301 gaatctccaa ttgaatgatc aagagttagt agatgacctc ggaaagacat tcaattatga
      361 ccgctacatg aatcagctga aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg
      421 ccgatattta gaatttttcc gttcttatgc ggaaaaacag attcctgttt tatatgtggc
      481 tttttccagt ggattaagcg gctcttacag tagtgcgctt caagcggttg aattgctgaa
      541 ggaagaatat gaagaccctg agatctatgt ttttgataca aaagctgcaa gtattgggga
      601 agggctttta gtattggaag ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac
      661 cctagagtgg ctggaaagta attatttgaa agtccattca tgggtaacag tagacgatct
      721 gaaatatcta gaacgaggcg gtaggatttc caaagcaagt gctactcttg gcagtctatt
      781 gagtgtaaag cctattatta cggtggataa agaaggaagg ctgacgaatg ttgacaaagt
      841 tcgaggaaga aacaagtcga tccagacaat cgtcaatgag acggtaaaaa caatcgttgc
      901 accacttgat caaatcgttt atgtggctta cgctggggat ctagagagcg cggagaaggc
      961 aaaacggctt ttggaagagc agataaagat gaaagatgtg aagctttatc ccttaggacc
     1021 aacgatcgcc agtcacacag gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg
     1081 ataaaagcag taactcattc tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga
     1141 ctcttaaaag atagatgagc atcctatctc ttagaggtcg ctgttttttt attttttaag
     1201 ctaaataggc cattatgccc ataactcgtt caattgtttt tgaatatctg cattttccaa
     1261 aaattcatca tatgtcgtat cagctcgatc gatcacacct ttatccgaaa cggcaatgat
     1321 gcggtttgcc aatgtttgga taaattgatg gtcatgtgac gcaaataaga ttgagccagt
     1381 aaatgccatc aagccgtcat tcaatgctgt gattgattct aagtctaaat ggttcgttgg
     1441 atcatctaat acaagtacgt tagctttcga aagcatcatt tttgaaagca tgcaacggac
     1501 tttctctcca ccagataaaa cattcactgg tttcaatact tcttcaccag aaaacaacat
     1561 tcttcctaag aagcttctta agaaggtgtt atcatcttct tctttacttg catattgacg
     1621 caaccagtct aaaatcgtta gatcagtatc gaattctttc gttgtatcct ttggtaaata
     1681 agaattactt gttgtcacac cccaacgtac tgttccagta tctggcgtaa tttcacccat
     1741 gataactttg aatagcgttg ttgttgttat atcattttct gcgacgaaag cgaccttgtc
     1801 ttctttgctc aaattaaaag atatatcatc taaaattttc tttccatcaa tcgttacact
     1861 tacattttct acctgcaata agtcgtttcc aatctcacgt tcaggtttga atccaacgaa
     1921 tggataacgt ctagaagaag gttggatatc gtctaaagta attttttcaa gcattttttt
     1981 acgtgaagtt gcttgttttg attttgatgc attcgcacta aagcgggcaa taaattcttg
     2041 gagttctttg atttgttctt cttttttggc atttgcgttt gcttgaagtt tagccgctag
     2101 ttgactagat tccaaccaaa aatcataatt tcctacataa agtttgattt taccaaaatc
     2161 tagatccgcc atatgtgtac atactttgtt caaaaagtga cggtcatggg atacaacgat
     2221 cacagtattt tcaaaattga tcaaaaattc ttccagccaa ttgatcgatt ggatatccag
     2281 accgtttgtt ggttcgtcta gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa
     2341 taacactttt accttttgtc caccagttaa ttcactcatt tgctgatggt gcaattcttc
     2401 agggatgttc agaccttgca gtaaaacagc tgcttctggt tcagcttccc atccattaag
     2461 ctcagcaaat tctccttcta attcagctgc tcgaatacca tcttcgtcag tgaaatcctc
     2521 tttcatgtaa atcgcatctt tttctttcag cacttcatac agacgcttgt gtcccatgat
     2581 gactgtttca agtacagtat agtcttcata gtcgtaatgg ttctgtttca atacggccat
     2641 ccgttcatct ggtcccatag taacggaacc tgtggaaggc tgcagatccc ctgataacac
     2701 tttaaggaac gttgatttac ctgcaccatt tgcaccaatc aagccatagc agtttccggg
     2761 ggtaaattta atattcacat catcaaatag tttgcggtct gaaaagtgca aactcacatc
     2821 atttacagta atcaattttt ttcctcactt ttatcattat tagttttgac aagtcttata
     2881 gaattatata ggggaaaggt aaaagattca aggcaatcga acattctttg tgtttcacgt
     2941 gaaacagaat gaaaaaaata aaaataaaag cggagcgaaa atagctacta taggatttat
     3001 tagtttgcaa cagcataatt tttgacgtta tttataagag ggagtactat aaaactgtaa
     3061 aagaaatgaa agaaggattg aaaatggatg aaaaaagaac catcgactgg ggctcattga
     3121 ttttgggaat tttatttgta cttgtctcat tgttatcatt tcaagaccct gtcggaaatt
     3181 tagttgcaat tgtagtagtc tttgcgattt ttgcctttat taaagggata tttgaactgt
     3241 ttgtacggaa cagattaaaa gagctaacag gatataaagg aaagatgccc ttaataatcg
     3301 ggatcatcga tattctagtt ggcgtctttt tcttattcaa tattggtgca ggagtcgcag
     3361 cattgccttt tgtttttgca gtatggttta ttgctgattc agttttagcg ttatttactg
     3421 ctgacttggc aagaggagta agtgaaggat attattggtt tacgatcatc gtcaatattt
     3481 taggaatttt attaggaatt ttcttattgt ttaatccaat ttcttctgcg ttgacattaa
     3541 gctttttagt tggattctat tttatgttat ttggtatcac acatatcgtc tatgcattta
     3601 gataagtaaa atcaaacaaa taaaaaagcc ggcttccgat tacagtttta ctgttgtaat
     3661 tggaagccgg cttttttatc atggacaaat atgcacgagg tgatttttag gtgtcgagac
     3721 aaatttatgc aaaaatgtga gaagctatat acacaaattt tatcttaata ctcgaagcat
     3781 agcgcagttg tcacaacctc ttttcatata attcattcaa tactggcaaa tcatggactt
     3841 catatttttc tttgaatgct tggatttctt cttcagaata ttttcttgga tctaaatgca
     3901 gatcttctac tggcaatggg cgcttgttat tgatcttcac atcgattact acaggacgat
     3961 ctgattttgc agctgcatca aaggcaaatc gaagttgcga gtattctgtg actgtaaagc
     4021 tatttgcccc gagcgcttct ccaactttac cgaaatctgc atcttctaaa aatacaccaa
     4081 atttcttttg ttccgtatct tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa
     4141 agacaacatt aataattgga agctgatatt tgacttgtgt aatgatatct tgcatgacca
     4201 tagcaaaccc gccatcacca cttaaggtaa atacttgttt ttctgggtag cttagttgtg
     4261 cggcaattcc tcctggcaca ccattaccca tagttgcgaa ccatccagat gtcgtatgtt
     4321 tttgtttacc gttcatattc aataaacgaa tagaatggat agtcgtgtta ccgacatctg
     4381 tgacgaaaat cgcatcgtct tcagcaatac gattgatttc tttatagaca gcctctggac
     4441 gaatcggttc ttcctctcga tcttcaaaag atttcaacca atttacccat ttttctttat
     4501 tttcttgatt tgctaggtac cagctatcag cagggcgagc atcccctaac tcgacaagtc
     4561 tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa
     4621 attttgacgc atcgatatcg atttggataa attcagcagt tggattaaaa aatgcacgtc
     4681 caaatgggaa gtcgctaccg acaaataaga tcagatcggc ttctgccaat gcttcatttc
     4741 cgggtttagt agcgacacgt ccagcaaaac caagaaagtt ttcataatca tcaggaataa
     4801 ttccttttgc tagaacggaa gcgacaacag gaactgaaaa atgttctgag aattctttga
     4861 tttgcggaaa tccgtttcgc gttccttgac caacataaag aactggtttt tttgcttttt
     4921 caagataagg caaagcagct agcaaatctt tttcttctgg taatatcaca cttgttttgt
     4981 gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc aaaaccaaga tcaacaggga
     5041 tagtcacgat agccacgcct ttatgctcat aggcggcttt aatggcttca tctactacat
     5101 gcggaagact ttgtggtgtc atgactgtac gattgtatac gctaacatct gcgaaaatag
     5161 gattttcatt tagttcctgg aaagagttat agttcatgga tgtagaagca acttgtccta
     5221 agaacgctac tagaggcaca tggtccatct gtgcatcata caaaccattg atcaaatgtg
     5281 tcgcaccagg tcctgcagag ccgaagacag cgccgacttt ccccgttaat tttgcatctg
     5341 cagcggctgc gagtgcgccg acttcttcat gacgaacttg aatgtagttg atttcggttt
     5401 tttcttgata tagcgcatcc ataattgaat tgaaagatcc tccgggaatg ccgtaaatat
     5461 ggtcgatacc ccagctttca agaactttta ccattgcgat tcctgcattg atttttgaca
     5521 tggtgtttca ctcctttagc aatgatgata cataaatttt ttgtatctag agtatagaac
     5581 tttttatcga aaatcccaac taattcgtct tttcacaaaa atgtagtcat gtttttggga
     5641 atatgctaga gtagtacata gatagattca agagagggga atggcatggt tgaatggcta
     5701 ttgagacttg aaccttggca gcaagcactt gctgggacag cttttactta ttttatgaca
     5761 gctcttggtg ccgggttggt ttttttcttt aaagaaatca aaaaagaagt gctaaacttg
     5821 atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt tttggtcttt gttagatccg
     5881 gctattacaa aagcagaaga aaatggagat attgcttggc tagttgtaag tattgggttt
     5941 ggtttaggcg gtgtgttttt atatatggca gataaaacac tgccccatat gcattttgga
     6001 ccacaacatg aagcagaagg attgcctacc catttaaaac ggacgatctt gcttgttttt
     6061 tctattacat tgcataacat tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca
     6121 gcaactgcgg ataatccgac agccgctata ttagccgctg tttcggtggc gctaggtatt
     6181 ggtatccaaa acttcccaga aggagcagct gtatcaatcc cgttgcgaca agaaggtctg
     6241 agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa tcgttgaacc aattgctgga
     6301 gtgattggag cgctactagt aacgaaagtg gagctgctgc ttccttatgc acttgctttt
     6361 gccgcaggag caatgattta tgtcgtggtg gaagaattga tccctgaagc acagcagaca
     6421 ttgtccagca aacgtcattt tgcagttttt ggcgtcatga gtggatttat cattatgatg
     6481 attttggatg tggcattagg ataaataaaa aaaatatata tgatatatca aatagggccg
     6541 cgaacttata tcgcgaccct atttttttag taagttatat cataatgaag tgaatggtat
     6601 catacattaa taatagtttt ttctatgtgt atttcctttg atccagcgaa caagccagat
     6661 gataagtgcg attgggatta gaactttgac cgcaaaccat aaaagcccgc ttaggacact
     6721 gacaaaaaga ctgatgacga agctagctgc aattagcact aaaagaacca tgaagatatt
     6781 taagaacata tttttttgac gcatacaaaa ccctcctctt gttcattctt tactatagaa
     6841 aaagtataac agggattgtt taaaaacacc tccaactgaa gactgatttt tacttcatct
     6901 acttaaatga tcaaacaatt gctttttctc tggtttatca tctaaaatag agcggaaaag
     6961 gaggagaata tcatgcctaa aaatcgtctt gaagcattta cagatgctgt aatcgcgatt
     7021 atcatgacta ttttagtttt agaacttcat gaacctaacg gagacacgtg ggaggcgctc
     7081 gctgcattag gttaccgatt ttttatctat attgtcagtt ttatcatttt agcgatttat
     7141 tggaacaatc atcatcattt atttcaagtt gtacataaga taaatggacg ggtcttatgg
     7201 gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc atggatcagt
     7261 gagtacatca atagcctggc tccagaaatg acttatggtt ttgttacctt gaccgctaat
     7321 attgcgtatt atatcttggc aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca
     7381 ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat cgattggttt gaatgtgtta
     7441 ggaattgtct tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt
     7501 ctagctgctt ggatcatccc aagtcgtacc atcgaatcac aatatcggta aaatgatccg
     7561 tgtttaaaat ttaagagaaa aatatagaaa aaataaataa acgatcaaat atccgagtga
     7621 tactagtatt caaagaggga tgcagatgga tcaaaaaaga attcatgtag gactgattac
     7681 tagttttgtc acgatgctta tttatttgga tcaaggtcat ttgacttgga cagtagttgg
     7741 tgtcgtagga tttttgacat tattcacagc aataaaaaaa taaacatctt tatgtaatct
     7801 gatgttctat ctatttaaga aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt
     7861 ttttatttgt ggaaaattta atttttagaa aagtgtatcg aaaaatagat agttagtttt
     7921 tccacagtgg ataacttcgt aagagataaa aataaaaaat tagtcaaaga agggcgtata
     7981 gatttctttt gataggagat atacaacaga aatgaataaa tattaacgtg ttttcaaaca
     8041 gtataaaacg gtgtggtttt tggaataaaa aaataacata tctcatttta cacttttgtt
     8101 attttatact taaaaaataa cgacacagaa aataccgtgt ctcagatttc tctactactt
     8161 ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa agtaggatca ggcagaatat
     8221 actggcagaa tcaaggagaa gggagcctaa ataaggagaa aaaccataga aaatataaag
     8281 taattactta taaaacaagc tttttataga aaaataatat acctgagtag ggtataaatt
     8341 aagacggaat tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc
     8401 tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa
     8461 ctgcaagtcg tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa
     8521 ccaatgactg aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt
     8581 tttttacgaa cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat
     8641 cagaaataag aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga
     8701 tagatgtttg tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt
     8761 tatcaaatca gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa
     8821 gaagcttaca aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac
     8881 attcaaaagc accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac
     8941 cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata
     9001 agtgagaaat tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa
     9061 taaataaatc cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa
     9121 agtataccat cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt
     9181 agttatcgta cgaaaattag aagctttttt tgttgggaat ccggtttctt taacaaagct
     9241 aagatagcga gttgcttgga aaagttgcta tcttttttct gtcaaaacga ctcggaatgt
     9301 tcgttttttg ttctaatttc tccaagttgt ttactttttg atactaagtt tatgaaaaat
     9361 caagaaatag ttgttatact aggtaatgct gacaaacggg attggcattt catcttggtt
     9421 cagctttttt tccaattact aggaaggagg gatagattgt tcaagactta ttttcgtttc
     9481 aataaaatac aagataatcc ttttcgtatc atgaatctga tgctacttat ctttattgta
     9541 ttatggttag gttacataaa tcatgatttt ttgatttcaa gtttttcatc attagggatc
     9601 tttacttttt tctattatca aaacataccg atgaaatatt tgctgaaacg tatgtttctg
     9661 atcggttgcg gactgttggt tgctttcgtt ttggggatct tatcgactta tgttttatgg
     9721 cttgaaccat ttgccgtagc tttggttgct ttttccagca gattcgtgtt gcgattattc
     9781 catatttcta aaccaggtgg cttgtttttt gcgatgcttt ctgccatggg aacgagcatg
     9841 cagctgccaa tcgctcaatt accgatcgtc tcactttatt tttttatggg tgtcgttttt
     9901 gccctgattg cggcagtgat tacaaaatta ctggattcac ggcctgagca gacaattgaa
     9961 aaagcaactt taaaagaaag atttcacgaa gagcctttag tgatcattga ttctgttttc
    10021 tacagcgctg ccttatttct ttccgtttat gtcagtcacg gcttgaatct gcataatcct
    10081 tattggttga ccttgtcatg tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag
    10141 catcgacaag tccaatatct gattggatcg atgggtgggt tatgtgtgtc ggcattcttg
    10201 tcatttgtgc cgtttacgca gttgcagacc atttttctga tcacttttct atatggaatc
    10261 gcacaatttc tagttgctag aaattatgca gttgctaata tttttcttaa tccgatggcg
    10321 ttgatgctat ctacacttat tcgtggagca tacttgattt ctttgatcga atatcgtttt
    10381 ttagggatcg tcataggaag tttcatcggt ttaggtgttg cgtgggtaat gacggttggt
    10441 ttgcaacatt atttaacggt tgttcgtcaa aaattagaat agaaggacaa atatatgtgc
    10501 caaaagaggt tatgacaaga tttttgtcac aacctctaat tcaaggtgtt caaaagccag
    10561 attctcagca ttaagtcaaa ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt
    10621 atatttattc tatcaaaaaa ttttgtttat gatggtaact gaataaactt tcctgagatg
    10681 gtcaatgctt tccttgaaag tcttacagat attctttctt catagaaagt ttaagtttta
    10741 tagttagtgt acaataaaag tatgtttata ccatatggtg tccggataat cagtcattta
    10801 aacaatgagg ttcaaagata tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat
    10861 ccaaattctg gcagaaactt taactcttcc caaagtcgtt atagagcaaa tgattgtaca
    10921 agcaaaaaac atccttgatg aggtgtttga gcaatatagt catgaactaa tggatcctaa
    10981 taaagcaaag gcgactgcaa agaaaataga caaagtctat gaagaggaac cttttgcagc
    11041 attagctatt tatctatatg cagcttctca aagttggaaa aaaatttatc aaccattgaa
    11101 gattccaaga actatctatt tggcaacaat gaatgctttt actagattta ttcatgaaca
    11161 tttccaagta acaggaagct atcgctttga ccgtggtttt tggatttggc attacactag
    11221 tggcttgatc tttcggattg gtgaattgga gtacgaaatg acatattttc cgaaagggta
    11281 cacggatcta agattagaag gaaagcagtg cctttctctt catattcctt ccgatgcaga
    11341 tttatctcag aataagataa gagatagtta cgaaagtgcc attcgttttt ttgaacttta
    11401 ttttcctgat taccattacc aagtgatgta cacagatact tggttactat cacccaatct
    11461 tactaaatgg ttgaaaaaag agtcaaaaat ctgtcttttt gcagccgatt atcgattatt
    11521 atcagttgat gaacaggacg atagtggagt cccttggatc tttggacgag tagatgccca
    11581 aatacatgat tatccagaaa gtacgagttt acagcgacaa gcaaaagagc aattgcttgc
    11641 aggcgagcac attggctctg gcttaggtat tcttacttca taagtttagt acttcgaaat
    11701 agtttaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    11761 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
    11821 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    11881 tttttagtgt tctatcgaac acacagtacg ttcaataggt aaactagcta taacaataga
    11941 tattaaagtt aacggacaag agggcctttt ttaggtagct aaaacgtaat aatgaaaatt
    12001 tatttccaga gataggagtc aatcagctag tctgatcgac tgttttaaca agttgacttt
    12061 cattttaact tttgatacca tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt
    12121 agcatgacta caatcaaaga agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa
    12181 gtgcataccg ccgcttgaaa agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca
    12241 tcaaagatgt gaaacggaat agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa
    12301 tagccgaaaa acgtttttcg gctattttca ataatcggtt tttgtgtaag gtttccgaaa
    12361 gaactgctgt tgattttcca tttttaacat atgataaatt ggtagaatct ttcttttcaa
    12421 attgatacca ttccctaatt ccagttatat agttaactac agaaccacaa agcacgtacg
    12481 tttataaaag tattatctgt agttattttt caattccctt tcaacctctt attttgacca
    12541 gtagtattga tcactttacc ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa
    12601 gaatggaaac gtaactacat aatcaaaggt tttgcgaaat atttcatgaa atatttcatg
    12661 tagaagcgag caatacggtt gcaaataacc attatcgttt aataagcagt tacgaaagct
    12721 cccaatatct ctaacgttca gctcactcca tttcttttct tcaaaaaaat cagtgattcg
    12781 tatctctttc tatttgtcga aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa
    12841 aaaaataaaa aagcagttac tattgctaga ataacatcaa ggaataataa gttttggttt
    12901 tcagataata gtcgataagt tgtgtattgg aattttagat acagtaataa agtatttttc
    12961 atgaaatttt gtgagaatca ctattacaag tatctttaat aaaattaatg gttaaggagg
    13021 aacttaaagt agaatgtaaa tgaatagttg tagtcagtcg attgtgatac tagactcaaa
    13081 gttgcaggaa taagtttttc aatttgtgat tggaagataa aaaatgaaaa agaagggctt
    13141 ttttttatac tttgttgatt gtagtgttta ttttccctct gtttagccaa gtaacatttt
    13201 ctatgactca accagtttca agtgaggaac atgataacct ttttcgtgct acagaagagt
    13261 ttaagaaata tcaggaaaat attgttccgg agactccatt agctagtgag ccaattactg
    13321 atattgatgg aaaccaaata ggatggtata tacagtacga agttatggtg gtaaataatg
    13381 actcaatagt aaaaaataca gtaggcttag agctaacacc gattgaaaag gtgagttcat
    13441 tgttaacgta tatttatact gatgaaagtg acgagactga gatcatttta acggattact
    13501 caaaaattgc tactgataaa aagtatatat tgtggatgta gacactagtg aaatacttta
    13561 tgatatagaa attgatgaaa atttactaga tgtagcacaa gaaatcgatc atcaaaagca
    13621 ggaagctatt cgatatgctg gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag
    13681 caaaagttag agaagatgaa aatgagtgga aagaattcaa gagtcgctac tcaattaact
    13741 cgactccgac atttacagtt tacagagaag gatcgataga aaaaactgtt ttttggacta
    13801 aagaaagtgg aatgtcttta gctgaagtag aagagttttt ggattatgtt agtatgcaac
    13861 agtaagggag ttcttattat gaaaaatttt gacacatatt atgttattgt tttttcgata
    13921 tctttgctgt taatattact aactttaatg cagtataaag agtatccaga gtattttatt
    13981 ttaacttttg tatatttagt aagtagctac acacttctat ataaattcag aagttcttct
    14041 agagtttcta aagtttgttt cttaattagc ttattgattc tataatattt ttgatttata
    14101 taggaaatta aaaatattat agaatcaaca ctcagagaac gggttggttt ttaatagatt
    14161 taataagtac tgataatgaa atttctcctg gttgggaaaa cgaagttggt taccctgtag
    14221 aagaattttt tcaagattac tttgcggagt atgccgatag gataatgaga gattacgtaa
    14281 cagataaaac tcaagaagtc atcaatcaat cccagtcatt aaatgaatta tctgataagg
    14341 cgaaagaaga agcagttgcc attatttctt cagggattag tgactatatt gatatgagtg
    14401 gtgaaatcaa ttatggcgat aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa
    14461 cttttgatgt gaaggcagat cttaagttta atgatactta cctagtagga aaattaatta
    14521 atatccttgg gacagagaaa gatacggaaa cgtttatttc ctctttgtct atgttgttta
    14581 cagataagca tttttcaact cagcttggtg gagaggaact agtggaaatc ttttaaccat
    14641 tacgcccagc attaataaaa gtatatcaaa aaagtactcc tgatgatttt taagattggt
    14701 ttgagaaata atgtgaaaac actattttta atgttaatta tagatagaga tgaacgcgtc
    14761 atatggttaa aagaatgaaa aagtcaaaga tacactgcta tatttcacta aggatatagc
    14821 agtgtatctt tcatgtcgtt tcactggcag ttttttataa aaggaggtgt gcatttcagt
    14881 tgcttttctt tggtagttag ttgaatcgtt taagaattaa cggatgtggc agacaggtgc
    14941 tctgtccaaa aaccagtaaa atctttgatg gttccatctt tatttagctg tgcctgttca
    15001 aatactgttt tgtttttacc gaatggatca attgcaataa ttgttccgat ccccgcacgt
    15061 ttggcagcaa gtaatcctga gtaagcatct tcgataacaa gacaatcagc cggagctaag
    15121 ctcagtttgt ctgccgcttt aagaaaaata tccggttgcg gtttcccagg gaaagagccg
    15181 tcgtcataga caatcttgtc ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa
    15241 aaagcaacat tctctttgac agtagccgtg gcaatcgtta atggaatctg ttggtcagtc
    15301 agctggtcaa gagtcgcttc aagccctttt gtcaactgaa gctcgttttc attttgcaag
    15361 caaagcatac gataatagct ttctttttct tctgacaata cggcgatctc agaggcggtc
    15421 aaatgctctg aaatgaaatg tttcaaaatc tccgtattcg tccgaccatg aatatttttt
    15481 aaaatatcct cgtctgataa accatctttt gtatgatcat gtatcatatg cagccaagca
    15541 gcttcatgca gatgagagtc caaaaacatt gttccgttga aatcaaatat aatccctttc
    15601 atagttacgc tcctatagtg gtattttcgg ttgaatcagc aatttgcaga tcgacatatt
    15661 gtgcatagtg ctcttgaacc tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat
    15721 catgcaaatt ataaaacaca taaaaatcat ctcgattgaa ttttttatta tcaacgagta
    15781 gatatttctt acgggaatta tttagagcga tctgctgtgc ttctccttct tcggtactgt
    15841 aagtagagat cgcttcgttg tgaacgccat ttgaactgat aaatgctttt gtaaacttta
    15901 gtcttcttaa attgtcgttg gtgatcggtc cgacaaatgc gccggtattt ttccggaaat
    15961 caccgccaat cagtagaatt tctggtccat ctgcttgagc aagaatatca aaaactggat
    16021 aattatttgt aatgatccga attttcttgt tcagtaaatg cgtcgccaat agttcgattg
    16081 ttgtacctgg tcctaagaaa atcgtttctc cctcgtgaat caaggaggct gctgtggcag
    16141 caatttcttt tttttcttga atctgcagag tggctttttc agtgtgagaa agttcttgat
    16201 ctaaagtgta ggaaagactt tgagcccctc catggacacg cagcagcttg cccgattttt
    16261 ctaattcatc taaatctctg cgaacggtca tatcagaaac atctaattga ttgatgattt
    16321 cattaacggt aaggattcct tttttattga ccatttctac gatagtaagc aatctttctt
    16381 ttttcaacat tgtcttcacc tctaatttaa ggataatcta tcatataaac ggacaacagt
    16441 caatctaatt cgaacaaaag taaacattta gggtggtttt tatgtttgtt acaatagatt
    16501 ttattgctgt tttctggttt ttatgattat ttgtgtttgt ttttgttgac aaatgtttcg
    16561 gatgcgttta caatgcaatc agttagaaag agcgaaaact gattatagat ttgaggagag
    16621 atgtacatgc ttaaattacc agaagatttt atttttggcg gagccacagc agcctatcag
    16681 gttgaaggag ccactaagga aggtggcaaa ggcgctgttg cttgggatga ctttttagag
    16741 gaacaagggc gattcagtcc cgatccggca agtgattttt atcatcaata tgcaaaggac
    16801 attgaattgt gtgaacgttt tggtgtaaat ggtctaagat tatccattgc ttggagccgc
    16861 atttttcctg atggagctgg gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg
    16921 ttcgaagaat gtaagaaaag aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc
    16981 cctaaacgtt tgtttgacca tggagatttt ttgaatcgag aaacaataga agcatttgtt
    17041 tcctatgcca ttttttgttt tcatgaattc aaagaagtca aggtgtggag tacctttaat
    17101 gaaatctatc cagttgcaac caatcaatat ttgttaggtg ttttcccacc aggaatcaaa
    17161 tatgatttta caaagatcgt cgcctgtctt cataatatga tggttgctca tgcccgtgta
    17221 gtgaactatt tcaaagaaaa cgaacttccg ggtgaaattg gtgtagttca ctcattagag
    17281 acgaaatatg cggcaacaga cgcgccggaa gataagcatg ctgccttttt agacgatgca
    17341 ttgtcgatcc gtttcttatt agatgctacc tatttaggct attattcaac tgaaaccttg
    17401 actgctcttg atgagatatg tgaagcaaat caggccagct atcattttcc tgaagaagat
    17461 ttcgttgaat tgaaaaaagc atccacgcgt aatgactact tagggatcaa tcactaccaa
    17521 tgccattttg tcaaagctta cgatggagaa aatgcaatcc atcataacgg tacgggtgaa
    17581 aaagggacct ctgtttataa agtgaaggga atcggagaac gtatttataa agaaggaatt
    17641 cctcgaactg attgggactg gctgatttat ccagaaggtc tatatgactt gttgctgcga
    17701 attaaaagcg attacccaca ttacaacaaa atctatatca ctgagaacgg gatggggtat
    17761 aaagatcagt ttgaagatgg cattattatg gatcagccga gaatcgacta tttgagagtc
    17821 tatttggaat ccttaagcaa ggcgataaca gccggggtaa atgtgaaggg gtattttttg
    17881 tggtcgctta tggatctttt ctcatggaca aatgggtata acaaaaggta tggcttgttt
    17941 tatgttgatt ttgaaaccca gaagcgttac ccaaaagaat cagcttactg gtacaagttg
    18001 gtcagtgaaa ccaaaacgat tatttaataa gtgataatgg aggaagaaaa gatgaataaa
    18061 gaggaattac aaatgcttgg ttttgagatc gttgcatatt caggcgacgc tcgcagtaca
    18121 ttattgaaat tattgaaaga agtaagacaa ggaaactttg aacatgttga ttcggcgttg
    18181 aaagaagccg acgaaaattt aacccttgca cataattcgc aaacaaaaat tttagcggaa
    18241 gaagcatctg ggaaagagat ggagatggga ttcattttta tccatgggca ggatcattta
    18301 atgaccactt tattgctgag agatctgatt caagacttta ttgtactgta cagacagaat
    18361 caaggatagg agaagaacgc tatggatgga atcattaaac aaatcgaaaa agggaaacca
    18421 ttttttgaaa aaatctcacg taatagttat ttaggagccg ttcgtgacgg atttttaaca
    18481 gcgatgccag ctattttatt ttcaagcatt ttcattttga ttgctgcggt tcctgagatt
    18541 tttggttacc aatggcctga atctgtctcc acatggctat ggaaagtcta taattattcg
    18601 atgggtgttg tagggttact cgtttcagca accactgctc gctgcttggc tgattcgatg
    18661 aatcgtcgct taccgaaaaa caaagtaatc aacaccacct ctgtgatgct ggcctctgtg
    18721 gtaagtttcc tgttgttaag cgtctctccg attgaaggtg gattttcaac ggaatatatg
    18781 gggacaaagg gactactggc ttcctttgtc gctgcatttt taacagttaa tttatataag
    18841 ttttgtgtgc tgaaagatat taccattaaa atgcctaaag aagttcctgg aacgatttcg
    18901 caaaccttcc gagatatttt tcctttctct ttttcagtat ttgcggccgt tttaattgat
    18961 ttaggcattc gacaaatctt ccaaatctca tttgcagaag caatcattac ggtgctgcag
    19021 ccgctattta cagcagctga cgggtactta ggaatcgcta ttatctgggg agcgatggcg
    19081 ctgttttggt ttgtcggtgt tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt
    19141 atctatgcca atgtggaaac caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa
    19201 gtactgacgg tgggtttagg gaattttgta ggaactatgg gaggaactgg ggcaacattg
    19261 gttgttcctt ttatcttcct actctttgct aagtctaagc agttgaaggc agttgggaaa
    19321 gcttccttta ttccagtaag tttcgctgtc aacgaaccat tgctatttgc ggcacccatg
    19381 attttgaacc cttatttttt tattccattt ttgttaacac ctgttgtaaa cgtttgcctg
    19441 ttcaagtttt ttgtcgatgt attgggcatg aacagcttta tgtacgtttt gccttgggca
    19501 actccagcac cgatcggttt ggttctagga acgggcatgg gtatcttagc ttttgttcta
    19561 gcagcggttc taatcgtcgt tgacttcctg atctatctac cattttgcaa agcatacgat
    19621 gctgtcttag tagagaaaga agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct
    19681 tctgccgatg tacgtgagga agtacctgtg gcagcgagtc aagttatcag cgatgcaaat
    19741 aaagaaatca atgtgttggt actttgtgct ggcgcaggga caagtgcgat gttagctaat
    19801 gctttaaaaa aaggtgcatc tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat
    19861 ggttctcatt acgaaatgct gcaggatttt gatatggtgg tcttagcacc gcaagtcaat
    19921 tcttactatg atgatatcaa agaagacacc gatcgcttag gaatcaagct ggttgcgaca
    19981 aaaggagcag aatatatcgg attaacgaga gatcctgaaa aagctgttca gtttattctg
    20041 aaccaatttc aataaaggag gagccataat gaaaatggga atttggttgc gtaaagctgt
    20101 tttatttgca gctgtgttag gggctttggg ggctgtttca aaaactgttg aagcatctgc
    20161 cagcgaattt attcgcggag cagatatttc gatcctgtca gatatggaaa aatctggtgc
    20221 aaaatattat gaagacgata tttcaaaaga tgccttagag attttaaaaa ataatggtgt
    20281 caactatgtt cgactgcgtt tgtggcagga tccttataat agtaatggag aaagttatgg
    20341 agcagggaca aatgatctgg aaacgacgat tgaccttgca aaacgagcaa aaaatctggg
    20401 acttaaggtg ctgctggatt ttcactacag tgatttttgg gttgatcctg ggaaacagaa
    20461 tcttcctaaa gcatggcaag gtctgacctt tgaagaaatg aatacggcat tgtacgacta
    20521 tacaaagaac gttctgtcgg aaatgaagca gctggacgtt tatcctgata tggttcagat
    20581 cggcaatgaa ctgaatagcg gaatgctctg gccttatggc aagtcttggg gagaaggcgg
    20641 gggagaattt gatcgattgg ctgctttttt gaagtctggt atccaagctg ttcgagacac
    20701 tcaacctaaa acaactccag taatgctcca cttagctgat ggtggcgata caggagcatt
    20761 tacttggtgg tttgatgaaa tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc
    20821 gtattatccg tattggcacg gttcaatgaa tgaacttcaa accaatatga atgcgatcag
    20881 ccaacggtat ggtaaagaag tgatcgtagt tgaaacagct tatgggcata caacagcgaa
    20941 tgccgatact atgccaaatg cttttggtga agccgaagca gctgcggggg gatatgaacc
    21001 aacaccgaca ggacaagcag agtatttgct ggatcttgcc gatcggattc aggcagtacc
    21061 gaataatcgc ggggcgggct tcttctattg ggaacctctt tggtacaacg gaaatgtgag
    21121 ttgggcaaca caagcaggta tgaattatct cagtgttcaa tccactatgg gaaacgaatg
    21181 ggacaaccaa gcgatgtttg attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa
    21241 acaagcaggg gcgcaaataa atctagttaa aaatgcaagt ttcgaaagcg atggaccaac
    21301 gaccagtcct tccaattggc aaacctggtt tcaacaagga accgctaagg gtacgatcaa
    21361 aacggaagcg ggcacttttt ttggggacta caaactaact ttttggcagg atcaagcata
    21421 cgaggcatca gcttatcaaa atgttagtgg cataagcaaa ggaacttaca cattgagtgc
    21481 ctatgtaatg tctgggggag agtttgatac taatcagtta tatatcaaaa attatggtgg
    21541 agcggaacta aatactgcgg ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga
    21601 cattccaatt acaacaggtg tttgtgaaat tggggtttat agcaaggctt caggaaatgc
    21661 ttggacgaac gttgattttg tttcattggt gaaacaatag actgcggatc gtttcgattc
    21721 aagatcggag gttcccttca gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac
    21781 ctcgcctttg ccaaaaataa ccagaaacga tttccagtca tttttaccaa aacgagtaaa
    21841 gcaccagact tcatctacac tttgtattat gctaaagaga gttcaacgat ttcgtaagaa
    21901 gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa gtttgtttaa gaaagggtaa
    21961 ccttagaatt ccgtcgtgaa tatgatttga gcacttagat ggatagaaaa aagttctcgg
    22021 aaaacagttc gtttttcgag aacttctttt tatgataaga atgaaacaaa ttaacttata
    22081 ttatagtctc ttttggtagg gaaccatctt atttttaatg aaaatcaagc attttagcta
    22141 ttgaagaatg ttgatttata gggattcccc ctccatatta tgttggctct ttattcccac
    22201 tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac gtgggctact
    22261 tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg ggcaaagtca
    22321 gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc gtaagtacgt
    22381 ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata ttgccagata
    22441 tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg
    22501 atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc aggaccaggg
    22561 aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa ttcacggact
    22621 tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc ttctggcagt
    22681 ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg
    22741 tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc aagttttgtt
    22801 gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt ttgttctggg
    22861 tcagatacac ctttcaactt gtctaagaag cgatctcttg cattgacttt gatgatgttc
    22921 aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt gcgtagcaga
    22981 ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca
    23041 acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc accaacttgt
    23101 tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt accttcacag
    23161 tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga atgacgtact
    23221 tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga agcaatcgga
    23281 caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac caagtcacca
    23341 tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc ttgatcgctt
    23401 gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac ggaaccgcca
    23461 agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat acctaaattg
    23521 aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg tccgccagaa
    23581 agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg gtggctcaat
    23641 aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt atactgacta
    23701 ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt
    23761 attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa aatgctaata
    23821 ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa ttatatcggc
    23881 tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat ttttccatac
    23941 atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat aataataatt
    24001 ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat accatttttc
    24061 gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc tgacgtagat
    24121 ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga tatcaggctg
    24181 ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt aaacaggtac
    24241 ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca tctccatatc
    24301 ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata tacgatttgg
    24361 ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg tacgagacaa
    24421 aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc cggcgatccc
    24481 gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac tcaagaacaa
    24541 agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat agatatctcg
    24601 aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt catcttgtgt
    24661 caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca cacggtagta
    24721 gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac tattttaaca
    24781 caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt ataatcttta
    24841 taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa aggatagact
    24901 gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta ttctgaacgc
    24961 ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag aacctttcaa
    25021 tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc acgtgtcctg
    25081 attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga tgtgggatgc
    25141 gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt tgaaatggtc
    25201 gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa
    25261 catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac ctatgcagct
    25321 atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc
    25381 gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa gaaacaagga
    25441 gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat cgtcacaaaa
    25501 gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa atagtggagc
    25561 gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt ttttgttgag
    25621 aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga aatagtagga acagagtcgt
    25681 tgttttttac gataaaataa ataagataca gaacttgtat agaaggctgc caagtcggta
    25741 gataaaggag gtttttatca aaaaacatac tttccttttt tctctattct tttcttgggg
    25801 gttttggtac actgataggg aatttagata gaagggagcc aataggatga agctgaccat
    25861 cagagatatc gcagaaatgg caggcgtatc agtaacaact gtctcacaga ttttgaataa
    25921 caaaggaagc agatttagtg ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca
    25981 tcattataag cccgattatt tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg
    26041 aatgatcgtc cctgatgtca ctgatttctt cttctccaaa ctgatcgaag gtgtagagag
    26101 ctatttgaat cctttgggct atgtgatcat gttatgcaat tctcgtcata gccaagaaaa
    26161 tgagatcaag tatttacaag agctttccca ccgctcagtg gacggaatct tgctggccac
    26221 accaaatatt ttacctgaga aatatgcatt agacagcggt ttttatcaaa aaatgccaat
    26281 cattttaatc gatcgcggat tgaatcgtcg ggataacgga cggcttatcg taaaggaata
    26341 tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat ggccatacga agatcggcat
    26401 gctgaaggaa accactggat actatcaatt agaagaacgt ttcaacggat atcgtcatgc
    26461 gctgaaagat catggcctgc cttttaatgg gaaatttgtt gaacatggag agttgactgt
    26521 ccaaggtgga tatactgcat caaaaaaatt actcaaacat aaagatatca cggctatttt
    26581 ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa
    26641 aatcccagaa gatatctcag tcataggatt cgatggatta aagctgtcag aatacatgat
    26701 tccgcaattg acgacagtgc agcagccaag ttttgatatt ggattttatg ctgcacgttt
    26761 cctgatcgat acgatcgaat ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa
    26821 attaattatt cgtgagagcg taaaagtatt gacaaagggc taactcggcg gtatattgct
    26881 tatgtaagca tttacaaagg ctagttagtg ctttttttat ttttagtaaa ccagtttact
    26941 aaagtggttt acctttccta aaaataatgt ataataccga atgaagacaa ggaggatcag
    27001 gcaatgcgaa tggtggatct gattgaaaaa aagagggatg gaaacgagct gtcgaaagag
    27061 gaaatcgagt acatcgtcac gaactatacg aatggaaaga ttccagatta tcaagtcagt
    27121 gcattattga tggcaatttt ttatcaagac atgacaaatg aagaaatcac gaacttaaca
    27181 ttggcgattg ctaattctgg tgatgtcatc gatttgagtt ctcttgaagg aataaaggta
    27241 gataagcatt cgacaggtgg cgttggtgac acgaccacat tgattttagc ccccctagta
    27301 gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg gtctaggtta cacaggtggt
    27361 acactagaca agctagaagc aattcccgga tttcagatcg agctttcgga tgaagaattt
    27421 gtacgcattg ttaatgaaag taaagtagcg gtaattggtc aatcaggaaa cttggcaccg
    27481 gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga ccgttgattc tcttccgctg
    27541 attgccagtt cgattatgag taaaaaaatc gcggcaggag cagatgcaat cgttttggat
    27601 gtgaccactg gagacggtgc tttcatgaag aacatcgaag atgcaagacg tctggcaaag
    27661 accatgacga gcattgggaa actggctaac cgagaaacag tggcggttat ctcagatatg
    27721 tcagaacctc ttggcgaagc tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca
    27781 ttacaaggaa atggtccaga agatctagta gaaatgtgct acgccttagg aagccaaatg
    27841 gttgttcttg ctggcaaagc taagacaacc gatgaagcaa gaacgttgct tcaagaagct
    27901 ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga tccaaaatca agggggagac
    27961 ccagcgatcg tggaacatcc agaacgaatt ttgactgcac gctacacaat ggagcttcct
    28021 gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg
    28081 atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg atcacgctgt tggattgaaa
    28141 ctgcataaaa aaattggtga cactgtgaca aaaggtgagt cactattgac gatctatagt
    28201 aacgacgaag aaatcacttc tgtcattgaa ttattatata aaaatattga aattggtgaa
    28261 tcggcaatga agccaacgtt gattcatgac atcatcacag aataaaggaa aaaagcggac
    28321 tttaccgttt taaagaaaag gggaaaagaa atggaactaa accgaatgat cgaccatact
    28381 attttgaaag cagacgcatc taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa
    28441 taccattttt attctgtctg catcaatcca gcatgggttg cgctagcgaa agaacaatta
    28501 caaggtgagc cagtagccgt atgtacagta atcgggtttc ctctaggagc aaacacaccc
    28561 gagacaaaag catttgaaac aacagatgcc atcaataacg gtgcagatga agtcgatatg
    28621 gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa
    28681 gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag ttattattga aacatcgtta
    28741 ttagatagag aagaaatcat ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt
    28801 gtcaaaacat caactggttt ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg
    28861 cgtgaaactg taggaccaga aatgggtgtc aaagcttccg gcggtatcca taacgaagaa
    28921 gaagcaatgg caatgatcga agctggtgct acaagaatcg gaacaagcgc aggagtagca
    28981 atcgtatctg gttcaactgg tgaaggctat taattttttc aaaggagaag ctcatggcag
    29041 aactcaaaca agaatggatc aacacagcta ttgaagcgtt agataaagcc tatgttcctt
    29101 attcccattt tccggtaggc gcttgtctcg taacggaatc aggcaagatt tatcaaggca
    29161 tcaacattga gaatgcatca tttggattaa cgaattgtgc cgagcgcaca gcttttttca
    29221 aagcagtatc tgaaggagaa aaaacattta cgcatctagt cgtagcgggg cacacccccg
    29281 atcctatctc tccttgtggt gcatgtcgac aagtaatggc agaattctgt tcccctgata
    29341 tgccggttac gctagtagga gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc
    29401 tgccttatgc atttactgaa aaagatctat aactcatgtt cattcagtca gctgactgtt
    29461 gaaaaataaa acaattattt taggaggctt tacagaaatg aaaaaagcaa aattatttgg
    29521 tttaggtgct gtcgcactag cagcagggtt atttttaggt gcatgcggca acaacggctc
    29581 tactgattca tccggtggca aaagctcatc agacacaaca actgcagcat tgatcacaga
    29641 cacaggtggt gtagacgacc gttcattcaa ccagtcagct tgggaaggac tagaaaaatg
    29701 ggggaaagat caaggacttt cacgaggaaa tgatggcttc caatatttcc aatcttcaaa
    29761 tgaatcagat tacatcccaa atattgacca agcattgaat gctggattca aaacgatttt
    29821 tggtattggt tataaactaa aaccagcgat ccaagaacaa gcaacaaaca atacaggtac
    29881 gaactttgtt attatcgatg atgtcatcga tggattagat aacgttgtat ctgcaacttt
    29941 caaagacaac gaagcttctt atcttgcagg tgttgcagca gcttatacga ctgaaacaaa
    30001 cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt
    30061 caaagccggt gttgatgcag gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca
    30121 atatgctggt gacttctctg ctccagataa aggacgttca attgctcaag gtatgtatgc
    30181 acaaaacgca gatatcatct tccatgcttc aggcggtaca ggtaacggtg tattccaaga
    30241 agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga
    30301 ccaatcagac gaaggcgaat acacattaaa cggcgaaaag aaaaacttca cattgacatc
    30361 tacactgaaa gcagttggaa cagtagtaga agatttggca caaaaatcag cggacggtaa
    30421 attccctggc ggcgaacaca ctgtttatgg attgaaagaa gacggtgttg gtctaacaga
    30481 aggacaactt tctgacgaag cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc
    30541 aggtgacgta aaagttccag aaactcctga agaaaactaa aaccatttct atagccttgt
    30601 tagacacatg tttgacaagg ctatggtttg attagaacaa gaattgttca tttatccaag
    30661 ctgccttgtg acagtttgga taagggaaca actccctgaa gtaatgtttg tttcaaaaga
    30721 agaacgctca ctaagcgtga atcttagtca atcgttgccc tagatagaaa tcgttaggat
    30781 gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat gcagaatatt accaaagaat
    30841 ttggaacatt caaggcaaat gacaacatca atttacaagt aaaggcagga gaaattcatg
    30901 cgttgcttgg agaaaacggt gctggcaaat ctacattgat gaacgtgctt tccggattat
    30961 tagagccgac atcagggaaa attttgatgc gtgggaaaga agtaaagatc acaagtccga
    31021 ccaaagccaa ccaattaggg attgggatgg tccatcagca ctttatgctt gttgatgcct
    31081 ttactgtaac agaaaacatc gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc
    31141 ataaaaaagc acgaaaagag atccaaaaag tttctgaaca gtatggattg tcagtcaacc
    31201 cagatgctta cgttcgtgat atttcagttg gaatggaaca acgggtagaa attttaaaaa
    31261 cactttaccg aggagcagat gtactgattt ttgatgagcc gacagctgta ttgacccctc
    31321 aagaaattga tgaattaatc gtgatcatga aggaattagt caaagaaggc aagtcgatca
    31381 ttttgattac gcataagtta gatgaaatca aagcagtagc tgaccgctgt acagttatcc
    31441 gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt aacctcacaa caattagccg
    31501 atatgatggt tggaagaacg gtttcattca aaacgatgaa aaaagaagcg aagcctcaag
    31561 aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga
    31621 aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg tatcgctgga atcgatggaa
    31681 acgggcagtc ggagttgatc caagctttga ctggtttgcg aaaggcagaa agcggacata
    31741 tcaagctaaa aggggaagac atcactaata aaaaaccccg aaaaatcact gaacatggtg
    31801 taggacacgt gccagaagac cgtcataaat acgggttagt cctagatatg acattatctg
    31861 aaaatatcgc actacaaacc tatcatcaaa aaccttacag taaaaacggc atgctgaatt
    31921 attcagtaat gaatgaacat gccagagaat tgatcgaaga atatgatgtt cgaacaacga
    31981 atgaacttgt tcccgcaaaa gctttatcag gcggaaatca gcaaaaagca attatcgctc
    32041 gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa tccaactcgt ggactggatg
    32101 taggtgcgat cgaatttatt cataaacgtc tgatcgaaca aagggacaaa tacaaagcag
    32161 tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta
    32221 tccatgaagg agaaattgtc ggtatcgttg atccgaaaga aacatctgaa aatgaattag
    32281 gtctattgat ggcgggctat accttagaag aagcacgaaa agagttagaa aagggggctg
    32341 gcgcacatga atgatcgcag cgaaaaattc cgtaatttat tagtgcctgt actttcagtt
    32401 gttttaggat tgatcttagg cgcgatattg atggctgctt ttggttttca gcccatccaa
    32461 ggttattctt ctatgctaga tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta
    32521 aggcaagcaa cgccgttgat ttttacagca ttaggttttt ctgtagctaa ctcagctggg
    32581 tttttcaata ttgggctttc tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg
    32641 gcacttgcgt ttccagatct gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt
    32701 gccttagctg gtgcgatggc agcggctgtt ccaggtttac ttcgagcatt ttttggaaca
    32761 agtgaagtga tcgtaacaat catgatgaac tatatcttat tatacgtaag cacatttgct
    32821 ttacatgaag tgatgcctga aagctatcgc tcaagtttgg actcttccaa tatgatcagt
    32881 caaaatgcct cactgcggtt accatggtta acacaagctt ttggcggctc tcgcgtaaac
    32941 tcaggattat ttttagcaat catcgcgctt attttcgttt ggctgctgat gaagaaaacg
    33001 actttaggat ttgaaatccg ttctgttgga ttgaatcctt ttgcttctga gtatgcagga
    33061 atgagtagta aacgtacgat cgttttgtcg atggtcattt ctggcgcttt ggcagggctt
    33121 ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct ttgttcaaac gacttcgcta
    33181 tctattggat ttgacggaat ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt
    33241 cttttatcag cgatcttgtt cagtattttg aagatcggtg gacttggcat gcagacagga
    33301 gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt tgattatctt ctttgtcggg
    33361 atcaacttcc tgattcgttt cgtcatggct aaagtcttcc aagggaaaaa acaagaagag
    33421 atcgttgcga caatagaatc gaaaccaacg aatcaaaacc atgaaggggg cgaactataa
    33481 tgtcgactat cgaattaatt gccttgatcc ttacttctac actggtttat tcaacaccgc
    33541 taattctaac gtcactaggc ggaacttttt ctgaacgaag cgggatcgta aacgtagggc
    33601 tagagggaat catggtcatg ggtgctttta gttccatcgt tttcaacttg acgatgagtc
    33661 catcacttgg ttctatgaca ccatggatcg ctattttagt gggtggcgtc gtaggtatcc
    33721 tattttctct gattcacgca gtagcaacag tcagtttgcg tgctgaccat attatcagcg
    33781 ggacagtaat taatctgatg gcacctgctt taggtgtttt cttgatcaaa gcatggtatg
    33841 gtaaaggtca aactgataac atcacgcaga atctggggta tttttctttc ccaggattag
    33901 cagatattcc ggtcattgga ccaattttct ttaaaaatac attcttacct gcttattgtg
    33961 ctattttgat tgctgtccta gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc
    34021 tccgttcagt tggtgaaaat cctcaagctg ccgacacatt aggtatcaat gtatatgcta
    34081 tgcgttattc aggcgtactg atttctggct tgttaggcgg tatgggcggt gctgtttttg
    34141 ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt tggacaaggg ttcatctcca
    34201 tggctgctat gatctttggt aagtggaatc ctttaggggc aatgggtgcc tcattgttct
    34261 ttggttttgc tcaaagcttg agtatcattg gcgcacaatt accagttatt tcttcgattc
    34321 cgccagtatt gctgcaaatt gctccatatt tgctgacaat cattgtgctg gttgttttct
    34381 tagggaaagc ttctggtcca aaagcaaacg ggaagaacta tatcaaatca aaataatctt
    34441 ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg tctcgccctt tttgttttaa
    34501 aggcaataaa aataaaaaga aagaaggaat aatcatgttt aaacgcgtac atctgatcgt
    34561 catggattca gtaggaattg gagaagcacc agatgccgat aagttcggtg atgttggttc
    34621 tgatacatta ggacacattg cgaaagaagc aggattgaac gttccacatt tagagcagct
    34681 tggtttagga acgatccgtc ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta
    34741 tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg atgacaggcc actgggaaat
    34801 catgggattg aatatcaaaa ctccatttcg tgtgtttcct aatggattcc ctgatgatct
    34861 gatcaaacaa atcgaagaat tttctggccg taaagtcgta tgtaacaaac cttatagcgg
    34921 aacagctgtt attgacgatt acggtgaaca ccaaatgaaa acaggcgatt taattgtata
    34981 tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga
    35041 attatatcgg atttgccaat tcgttcgtga tataacaaaa gatgatccgt atatgattgg
    35101 ccgaatcatt gcacgtccgt acgtgggtga accaggagac ttcacacgaa caagcaatcg
    35161 ccatgattat gcgttgaatc cattcggaca cacagtcctt gattctttga aagaagcagg
    35221 aaaggacgtg attgctgttg gaaagatcaa cgatattttc aacggacaag gaatcactga
    35281 atctgtccgc acgaaaagca atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa
    35341 cgatttttca ggaatcagct ttaccaattt agtagatttt gatgcattat acggtcatcg
    35401 tcgggatgta gtcggctatg cgcatgcgat tgaagaattt gacttacgaa ttccagaatt
    35461 gcttgaagcg atggaagaag acgacttgct aatgattaca gccgatcacg gaaacgatcc
    35521 gacgtttact ggtaccgatc atacgcgaga atatgtaccg ttattggttt acagtaaaaa
    35581 aatgaaagaa caaggggcat tgcctcaagg atattattca gatatttcag caacgattgc
    35641 tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata
    35701 atctttttaa ataggagaaa gcaaatgacc aagttaagcg agatgttaca agaaacgacc
    35761 cagtttatcc ttgataaagg agtaaaagaa attgattttg gtttgattct aggttcagga
    35821 ttaggtgaat tagcagatga aatcgaagat gctgtaatga tttcttatga tgagatcccg
    35881 ttctttcctg tttcgacagt agttgggcat gcaggaaagc tagtttatgg gtctctggca
    35941 ggcaagaaag tgttagccat gcaagggaga ttccactact atgaaggtca ttccatgcaa
    36001 acagtgacct atccagtacg tgtgatggcc ggactagggg cccattcagt cattgtgact
    36061 aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag atttgatgct gattaccgat
    36121 catatcaatt ttactggtga aaatcctttg atcggtccga atgaagatgc aatgggccct
    36181 agatttccag atatgagtca agcttatacg cctgactatc aagcaacagc aaaagcagca
    36241 gcagaaaaac taggtttgga tcttaaagaa ggcgtatata tgggctattc tggaccaact
    36301 tatgaaacgc ctgcggagat ccgaatgtct cggacaatag gagccgatgc agtagggatg
    36361 tcgactgttc cagaagtcat cgtagcagct cacagcggat taaaagtgtt aggaatctct
    36421 tgtattacca acttagcagc aggtatgcaa gcgaatctga gccatgaaga agttgtagaa
    36481 acaactcaga gagtcaaaca atcttttaaa gcattggtta aagaaacatt gacattattg
    36541 taataggagg aacaataaaa tgagtgtgca tattgaagcg aaagaaggcg agatcgccga
    36601 taagatttta cttccgggag atcctttgcg agctaaatat attgcggaga cttttttgga
    36661 agatccagtt tgttacaatc aggtacgcgg tatgttaggc tttaccggca aatataaagg
    36721 agaacgtatt tctgttcaag gaacaggaat gggaatgcca tcagccacca tctatgccca
    36781 tgaattgatc cagtcgtatg gtgtaaagaa attgattcga gtaggtacat gcggtgcatt
    36841 gtcaaaagac gtccatgttc gtgatttagt actagcacaa ggtgcagcga ccagttcttc
    36901 gatgatcgaa aaaaatttcc aagcctttca ttttcctcca atcagtgatt tcaatctatt
    36961 actgaaagca tatgagatag ctaaagaaaa gggctatacg gttcatgtag gaaatgttct
    37021 ttcggaagat tctttctaca aagatgattt gacagagacg ttccaattag ctgaacttgg
    37081 cgttttagga gtggaaatgg aagcagcggc tctttattac ttaggcgcga aataccatgt
    37141 gcagacatta agtttgatga ccgttagcga ccatttgatc accggtgaag aaacaacagc
    37201 tgcagaacgt caatctacgt tcaatgaaat gatcgaagta ggattggaaa cagcgatagc
    37261 agaataagaa ggaaatgaaa aagctgccct gcattaagta ataagaacat ccaagcaaaa
    37321 atagctcctc atgtttttga cttggctgga cttattattg caaatgtaag cttttgaaca
    37381 ccgtttacca agaggttgtg aa
//
LOCUS       DACETN010000018        35882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DACETN010000018 DACETN010000000
VERSION     DACETN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.019"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1152
                     /locus_tag="IXF98_000814"
     CDS             <1..1152
                     /locus_tag="IXF98_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555147.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXF98_000815"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXF98_000815"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1555148.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IXF98_000816"
     CDS             2728..3003
                     /locus_tag="IXF98_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1555149.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IXF98_000817"
     CDS             3009..3776
                     /locus_tag="IXF98_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1555150.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IXF98_000818"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IXF98_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1555151.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IXF98_000819"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IXF98_000819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1555152.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IXF98_000820"
     CDS             7920..8222
                     /locus_tag="IXF98_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1555153.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IXF98_000821"
     CDS             8225..9388
                     /locus_tag="IXF98_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1555154.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IXF98_000822"
     CDS             9414..12842
                     /locus_tag="IXF98_000822"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1555155.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IXF98_000823"
     CDS             12863..13987
                     /locus_tag="IXF98_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555156.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IXF98_000824"
     CDS             13977..14414
                     /locus_tag="IXF98_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1555157.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IXF98_000825"
     CDS             14398..14736
                     /locus_tag="IXF98_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1555158.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IXF98_000826"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IXF98_000826"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1555159.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IXF98_000827"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IXF98_000827"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1555160.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IXF98_000828"
     CDS             15893..16936
                     /locus_tag="IXF98_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1555161.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IXF98_000829"
     CDS             17095..17823
                     /locus_tag="IXF98_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1555162.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IXF98_000830"
     CDS             17939..18184
                     /locus_tag="IXF98_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1555163.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IXF98_000831"
     CDS             18181..18399
                     /locus_tag="IXF98_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1555164.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IXF98_000832"
     CDS             18535..19074
                     /locus_tag="IXF98_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1555165.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IXF98_000833"
     CDS             19067..21340
                     /locus_tag="IXF98_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1555166.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IXF98_000834"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IXF98_000834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1555167.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IXF98_000835"
     CDS             complement(22513..22767)
                     /locus_tag="IXF98_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1555168.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IXF98_000836"
     CDS             complement(23167..23436)
                     /locus_tag="IXF98_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555169.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IXF98_000837"
     CDS             complement(23906..24361)
                     /locus_tag="IXF98_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1555170.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IXF98_000838"
     CDS             complement(24499..26427)
                     /locus_tag="IXF98_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1555171.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IXF98_000839"
     CDS             complement(26624..27457)
                     /locus_tag="IXF98_000839"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1555172.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IXF98_000840"
     CDS             complement(27649..28353)
                     /locus_tag="IXF98_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1555173.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IXF98_000841"
     CDS             complement(28376..29002)
                     /locus_tag="IXF98_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1555174.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IXF98_000842"
     CDS             29328..31703
                     /locus_tag="IXF98_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1555175.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IXF98_000843"
     CDS             complement(31727..32218)
                     /locus_tag="IXF98_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1555176.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IXF98_000844"
     CDS             32405..33325
                     /locus_tag="IXF98_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1555177.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IXF98_000845"
     CDS             33479..33823
                     /locus_tag="IXF98_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1555178.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IXF98_000846"
     CDS             complement(34013..34513)
                     /locus_tag="IXF98_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1555179.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IXF98_000847"
     tRNA            35041..35124
                     /locus_tag="IXF98_000847"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IXF98_000848"
     CDS             complement(35448..>35882)
                     /locus_tag="IXF98_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1555180.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACETN010000019        35660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DACETN010000019 DACETN010000000
VERSION     DACETN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.020"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..2198)
                     /locus_tag="IXF98_000849"
     CDS             complement(<1..2198)
                     /locus_tag="IXF98_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555181.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
     gene            complement(2185..2403)
                     /locus_tag="IXF98_000850"
     CDS             complement(2185..2403)
                     /locus_tag="IXF98_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555182.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2405..3448)
                     /locus_tag="IXF98_000851"
     CDS             complement(2405..3448)
                     /locus_tag="IXF98_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1555183.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3603..3929)
                     /locus_tag="IXF98_000852"
     CDS             complement(3603..3929)
                     /locus_tag="IXF98_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555184.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3937..4140)
                     /locus_tag="IXF98_000853"
     CDS             complement(3937..4140)
                     /locus_tag="IXF98_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555185.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4215..4865
                     /locus_tag="IXF98_000854"
     CDS             4215..4865
                     /locus_tag="IXF98_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1555186.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4920..6086
                     /locus_tag="IXF98_000855"
     CDS             4920..6086
                     /locus_tag="IXF98_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1555187.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IXF98_000856"
     CDS             complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IXF98_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1555188.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IXF98_000857"
     CDS             complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IXF98_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1555189.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7545..8012)
                     /locus_tag="IXF98_000858"
     CDS             complement(7545..8012)
                     /locus_tag="IXF98_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1555190.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8132..9670)
                     /locus_tag="IXF98_000859"
     CDS             complement(8132..9670)
                     /locus_tag="IXF98_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1555191.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9750..10436
                     /locus_tag="IXF98_000860"
     CDS             9750..10436
                     /locus_tag="IXF98_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1555192.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IXF98_000861"
     CDS             complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IXF98_000861"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1555193.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IXF98_000862"
     CDS             complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IXF98_000862"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1555194.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11922..13052)
                     /locus_tag="IXF98_000863"
     CDS             complement(11922..13052)
                     /locus_tag="IXF98_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1555195.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13052..15235)
                     /locus_tag="IXF98_000864"
     CDS             complement(13052..15235)
                     /locus_tag="IXF98_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1555196.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15232..16257)
                     /locus_tag="IXF98_000865"
     CDS             complement(15232..16257)
                     /locus_tag="IXF98_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1555197.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16302..17129)
                     /locus_tag="IXF98_000866"
     CDS             complement(16302..17129)
                     /locus_tag="IXF98_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1555198.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17126..18193)
                     /locus_tag="IXF98_000867"
     CDS             complement(17126..18193)
                     /locus_tag="IXF98_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1555199.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18219..19055)
                     /locus_tag="IXF98_000868"
     CDS             complement(18219..19055)
                     /locus_tag="IXF98_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555200.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19052..19921)
                     /locus_tag="IXF98_000869"
     CDS             complement(19052..19921)
                     /locus_tag="IXF98_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555201.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19991..21274)
                     /locus_tag="IXF98_000870"
     CDS             complement(19991..21274)
                     /locus_tag="IXF98_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555202.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21288..22973)
                     /locus_tag="IXF98_000871"
     CDS             complement(21288..22973)
                     /locus_tag="IXF98_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1555203.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23248..24273
                     /locus_tag="IXF98_000872"
     CDS             23248..24273
                     /locus_tag="IXF98_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1555204.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IXF98_000873"
     CDS             complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IXF98_000873"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1555205.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IXF98_000874"
     CDS             complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IXF98_000874"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1555206.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32945..33913
                     /locus_tag="IXF98_000875"
     CDS             32945..33913
                     /locus_tag="IXF98_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555207.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33931..34836
                     /locus_tag="IXF98_000876"
     CDS             33931..34836
                     /locus_tag="IXF98_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555208.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34833..35639
                     /locus_tag="IXF98_000877"
     CDS             34833..35639
                     /locus_tag="IXF98_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1555209.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10110 a         7532 c         6433 g        11585 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttc ctcacgattc attgcttcag gcgtgtattt tgcttcctgt
      301 cgcttctgat taattaatac ttcagcagac ttagcttgac cgtgcatagc atttttacgt
      361 tcttcaatat cttgtttaac tttttgctga attgcacttg caggattaat atttgattca
      421 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcagcgttt
      481 tcaactttag cgccatcttt tctatccgaa agacgtgttg gcatagaatc agcatatttt
      541 ccagtttcat cttctttgtt tttagctact ataggtttgc tatttttatt aatagcgtac
      601 acggtaccat catctaaacc ttctttgaat ttattcttgg tatcattagc catatcacgc
      661 atactcttaa atggttttgt tgctctttca ctggccattg tagctccatc cgaagctaag
      721 cctgataatc ctcgttcacg agcataccca agtgttttcc ccgttttatt agctttttga
      781 gaaagtgatt gaccaaagtt gcgttcaggt tttcgttctt ccccttccgc ttgatcgttt
      841 ccaccactat ttttcccacc tttagatcgg ttcataccac ctgttccttt acgaaacagc
      901 atagctccca tgccaaatgc tgaagcaagc tggccatagc cttcttttaa accaatatcc
      961 acgccaaagt aacgcatgac tgttccacta ccctttatca gcacaataac agcgcagacg
     1021 tacgctactg cttttataaa aggatttaca gaagaaccgc tacctgttcc gagataatta
     1081 acaaacattg caaacataga caatccgaac ccttgaaatc cgactgtcaa ataacactgt
     1141 aaaatatctg acaaaacttg ctttgatcgt tggcctgttt ccaagtcagt agcaaatact
     1201 aaaactccaa gtacgcgttt aaacgctagc tcgagtattg ctgtaattat aatgaaagca
     1261 ctaaaaacat acgctaccgc taaagcaatc aatccaaaag aaatccccca tagatttgct
     1321 tgatagcgat aatatcctga tttaatgccc tcaacaaagg tcgacagaaa attatccgaa
     1381 aatttggtag caacaaattc attatcagaa ttttcaacta attcataacg cagattatca
     1441 gctattgggt tctctttttc cattttgtcg agtacattat ctgtcaatac ttgcgtaaga
     1501 tccgttttcc aaaaattttc ttttgttaga ttgttccggt cattcgtttt atcgatcatg
     1561 gaatattgat ctgttttatt gatataagct aaatcagtaa ccccttgttc aatcagcgac
     1621 cacgccacgc cttcgtccga atttgtaatc gtagtcgtat cattataaaa tgttttagaa
     1681 aactctattc cgctgtgaat caacgttggc attaaaagaa tcaatgaaat tgacattaca
     1741 acattcattc caacagattt tagatcaacc gttcccttac ctgtaatcat tttataacca
     1801 ataatcacta gtgaaagtat cattaagcca acaacaatgg tacttaaaac agcttgatag
     1861 actttgttca atccggtact ttctacaaag ctaaacaaat ctaaaacttc gggaatcata
     1921 ttttgaatgc catcgacaac agcgaacatt gattttataa cagaccagcc tatccacata
     1981 ccgaactggc tgtaaaaatt tccgttgtat tttttcaaat aattctggta aagcggatta
     2041 gttaaaaact cagtttgact ctcagtatca tcaaaccaat tgaaaatact gccttctgca
     2101 atgttactcg taaaaagaat cacagctatt agaacaagaa aaataaccgg taaccaattt
     2161 gtttttctat tagcaatctt ttttttaatt attttcattg ttacctccct cagcccattt
     2221 tcttaggctc tcagagagtt tctgagctat ttttctgtca attatgatat gagttacgta
     2281 atcatcatta acagctttta aatcgagaac aatagtattt gagcagatac tctcggttaa
     2341 ctcaagagta tctgcgtttt gatcaagaca gtgaatctta tctactagat gattttcaat
     2401 cattttaaat tccctctttt ttttgtagct tgattagttc taacgctttg ttttttagag
     2461 aagtaaagat ataattattt ttttgtttga tacgatgatc tgtttttgct cttcgtatca
     2521 agtcacgtaa agtcattgct agctgttctc taacatgatc tacaaaataa ctattattac
     2581 aaatttcata gataattttc agttgaactt tttgttcttt ttcagcagat ttaattgcat
     2641 aattgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta gccagtttta
     2701 agcaattcac aatactttct ccccacattt gtttaagaag attttcttca ataaatgttt
     2761 cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaaa agatcatcat
     2821 tatgtgattc tttaatatct ttactatctt tataagactt atataattct agttcatttt
     2881 ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg catagttcaa
     2941 attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga actatcaagt
     3001 ttttttgact attttcttga tgggttatct tattaatttt ataaacatca tctttgcaca
     3061 gtacaggctt taacaaataa taaaaatttg ggaacatttg cattttattt ttattgaagc
     3121 cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct gataagattg
     3181 tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc aaaaaaattt
     3241 tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct tttagtaaca
     3301 tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg tttaaaaaaa
     3361 taggatattg atagtataat tctgcatata tatcagaatt agaatagcct tgcttttcaa
     3421 ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt atttgctata
     3481 atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg atctgcaaac
     3541 tgacgggaac actcttttta attttttcta cttcatcatt attcaaaaaa acacctcgtt
     3601 ttttaaattt gatgactcaa tatccatttt ttgcattctt cttttgaaaa caactccatt
     3661 ttttctaata aaataactgg caatcccatt cttttaaatt tcaaaaaagt tgtatatgat
     3721 acattcaaat atttagccat ctcttttcct tttagaaatg gtctttctaa cccggtatca
     3781 attcttgctt gatcaatagc ctcttttgtc atttcaaaaa cataattttt caattgattc
     3841 gtttgttgtt tatctaacat tactgtaatt cctgcatcca ttatttttct cctctcattt
     3901 tctatttctt caatcagcca actcgccaag ttgtatttat ttagatttca aatcattaag
     3961 taaccaattt accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc
     4021 gattttgaca tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag
     4081 tataagatcg gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat
     4141 tcagcgcctc cttttaaatt attcactttg tggtttttat cttaataagc acattttaaa
     4201 ccacgaaacg agtaatgtca attaaaacat tactcaattt gtggttttta tgctataata
     4261 gtttggaatt tactataggg ggtgttactt tgaatagaat aaaattttat attgattcca
     4321 gtaaaaagac cattaaacaa attagtacag aaactggtat taactataca actcttacga
     4381 actattaccg tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt
     4441 atttccatgt tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac
     4501 ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata
     4561 ttgccagtct tgactcagat tattttactg aattatctaa tctatacttt tccatgactt
     4621 ctttacttga agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc
     4681 tagaggactc ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag
     4741 ctttaaactt aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa
     4801 cccaagtaca ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc
     4861 aataaaacta attattcaat cagccaactc gccaagttat cagattggag agattagtta
     4921 tgtcaataca aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc
     4981 gtaaagatga aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag
     5041 aagccaagtt acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc
     5101 cttctaatga cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg
     5161 tacaagaaac gacatatcaa cgtacaaaag accttttccg cctgcatata ctaccaaaat
     5221 tcgggaataa gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat
     5281 gggctttaaa attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt
     5341 atgccatttt tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc
     5401 gggaaaagaa agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata
     5461 ttctcaataa agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg
     5521 gtatacgtaa aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc
     5581 taatgtatgt tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa
     5641 aaactgtcag gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg
     5701 atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc
     5761 agtttggcat aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg
     5821 gtgacattaa tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga
     5881 agaaatataa atttaagcac gttactattc atggattccg gcacactcac gctactcttc
     5941 tactagaagg tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg
     6001 aaactaccct taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa
     6061 aattcattgc ttacactggt ttataatttt gtcattacct ttctcattac cagaacgaaa
     6121 aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg
     6181 ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     6241 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     6301 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     6361 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     6421 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     6481 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     6541 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     6601 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     6661 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     6721 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     6781 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     6841 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     6901 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     6961 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     7021 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     7081 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     7141 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     7201 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     7261 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7321 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     7381 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7441 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     7501 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     7561 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     7621 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     7681 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     7741 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     7801 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     7861 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     7921 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     7981 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     8041 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     8101 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     8161 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     8221 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     8281 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     8341 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     8401 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     8461 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     8521 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     8581 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     8641 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     8701 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     8761 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     8821 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     8881 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     8941 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     9001 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     9061 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     9121 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     9181 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     9241 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     9301 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     9361 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     9421 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     9481 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     9541 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     9601 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     9661 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     9721 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     9781 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     9841 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     9901 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     9961 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
    10021 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
    10081 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
    10141 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
    10201 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
    10261 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
    10321 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
    10381 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
    10441 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
    10501 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
    10561 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
    10621 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
    10681 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
    10741 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
    10801 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
    10861 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
    10921 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
    10981 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
    11041 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
    11101 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
    11161 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
    11221 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
    11281 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
    11341 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
    11401 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
    11461 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
    11521 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
    11581 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
    11641 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
    11701 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
    11761 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
    11821 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
    11881 tacaagaaca ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
    11941 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
    12001 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
    12061 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
    12121 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
    12181 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
    12241 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
    12301 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
    12361 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
    12421 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
    12481 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
    12541 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
    12601 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
    12661 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
    12721 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
    12781 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
    12841 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
    12901 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
    12961 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
    13021 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
    13081 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
    13141 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
    13201 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
    13261 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
    13321 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
    13381 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
    13441 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
    13501 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
    13561 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
    13621 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
    13681 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
    13741 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
    13801 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
    13861 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
    13921 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
    13981 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
    14041 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
    14101 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
    14161 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
    14221 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
    14281 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
    14341 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
    14401 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
    14461 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
    14521 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
    14581 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
    14641 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
    14701 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
    14761 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    14821 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    14881 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    14941 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    15001 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    15061 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    15121 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    15181 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    15241 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    15301 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    15361 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    15421 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    15481 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    15541 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    15601 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    15661 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    15721 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    15781 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    15841 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    15901 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    15961 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    16021 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    16081 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    16141 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    16201 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    16261 tttctccctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    16321 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    16381 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    16441 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    16501 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    16561 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    16621 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    16681 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    16741 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    16801 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    16861 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    16921 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    16981 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    17041 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    17101 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    17161 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    17221 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    17281 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    17341 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    17401 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatccctc
    17461 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    17521 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    17581 aatcggcacc gtatttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    17641 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    17701 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    17761 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    17821 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    17881 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    17941 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    18001 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    18061 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    18121 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    18181 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    18241 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    18301 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    18361 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    18421 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    18481 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    18541 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    18601 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    18661 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    18721 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    18781 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    18841 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    18901 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    18961 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    19021 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    19081 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    19141 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    19201 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    19261 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    19321 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    19381 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    19441 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    19501 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    19561 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    19621 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    19681 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    19741 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    19801 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    19861 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    19921 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    19981 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    20041 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    20101 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    20161 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    20221 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    20281 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    20341 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    20401 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    20461 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    20521 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    20581 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    20641 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    20701 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    20761 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    20821 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    20881 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    20941 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    21001 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    21061 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    21121 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    21181 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    21241 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    21301 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    21361 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    21421 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    21481 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    21541 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    21601 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    21661 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    21721 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    21781 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    21841 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    21901 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    21961 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    22021 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    22081 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    22141 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    22201 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    22261 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    22321 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    22381 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    22441 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    22501 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    22561 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    22621 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    22681 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    22741 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    22801 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    22861 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    22921 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    22981 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    23041 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    23101 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    23161 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    23221 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    23281 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    23341 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    23401 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    23461 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    23521 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    23581 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    23641 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    23701 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    23761 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    23821 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    23881 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    23941 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    24001 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    24061 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    24121 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    24181 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    24241 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    24301 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    24361 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    24421 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    24481 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    24541 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    24601 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    24661 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    24721 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    24781 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    24841 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    24901 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    24961 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    25021 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    25081 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    25141 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    25201 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    25261 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    25321 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    25381 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    25441 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    25501 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    25561 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    25621 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    25681 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    25741 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    25801 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    25861 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    25921 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    25981 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    26041 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    26101 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    26161 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    26221 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    26281 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    26341 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    26401 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    26461 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    26521 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    26581 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    26641 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    26701 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    26761 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    26821 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    26881 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    26941 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    27001 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    27061 tgatttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    27121 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    27181 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    27241 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    27301 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    27361 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    27421 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    27481 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    27541 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    27601 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    27661 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    27721 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    27781 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    27841 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    27901 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    27961 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    28021 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    28081 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    28141 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    28201 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    28261 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    28321 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    28381 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    28441 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    28501 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    28561 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    28621 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    28681 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    28741 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    28801 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    28861 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    28921 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    28981 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    29041 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    29101 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    29161 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    29221 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    29281 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    29341 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    29401 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    29461 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    29521 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    29581 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    29641 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    29701 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    29761 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    29821 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    29881 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    29941 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    30001 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    30061 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    30121 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    30181 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    30241 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    30301 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    30361 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    30421 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    30481 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    30541 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    30601 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    30661 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    30721 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    30781 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    30841 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    30901 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    30961 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    31021 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    31081 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    31141 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    31201 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    31261 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    31321 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    31381 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    31441 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    31501 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    31561 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    31621 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    31681 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    31741 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    31801 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    31861 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    31921 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    31981 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattccc
    32041 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    32101 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    32161 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    32221 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    32281 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    32341 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    32401 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    32461 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    32521 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    32581 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    32641 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    32701 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    32761 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    32821 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    32881 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    32941 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    33001 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    33061 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    33121 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    33181 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    33241 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    33301 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    33361 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    33421 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    33481 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    33541 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    33601 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    33661 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    33721 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    33781 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    33841 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    33901 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    33961 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    34021 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    34081 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    34141 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    34201 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    34261 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    34321 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    34381 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    34441 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    34501 tctaatggat gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat
    34561 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    34621 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    34681 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    34741 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    34801 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    34861 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    34921 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    34981 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    35041 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    35101 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    35161 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    35221 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    35281 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    35341 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    35401 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    35461 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    35521 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    35581 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    35641 cagaaagagg atgggacaaa
//
LOCUS       DACETN010000020        34672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DACETN010000020 DACETN010000000
VERSION     DACETN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.021"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(313..2205)
                     /locus_tag="IXF98_000878"
     CDS             complement(313..2205)
                     /locus_tag="IXF98_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555210.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IXF98_000879"
     CDS             complement(2202..3929)
                     /locus_tag="IXF98_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555211.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IXF98_000880"
     CDS             complement(3945..4595)
                     /locus_tag="IXF98_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1555212.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IXF98_000881"
     CDS             4799..5095
                     /locus_tag="IXF98_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1555213.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IXF98_000882"
     CDS             5287..5907
                     /locus_tag="IXF98_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1555214.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IXF98_000883"
     CDS             complement(5999..7165)
                     /locus_tag="IXF98_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1555215.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IXF98_000884"
     CDS             complement(7177..7530)
                     /locus_tag="IXF98_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1555216.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IXF98_000885"
     CDS             7658..8680
                     /locus_tag="IXF98_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1555217.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IXF98_000886"
     CDS             complement(9119..9553)
                     /locus_tag="IXF98_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555218.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IXF98_000887"
     CDS             complement(9663..11522)
                     /locus_tag="IXF98_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1555219.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12800)
                     /locus_tag="IXF98_000888"
     CDS             complement(11640..12800)
                     /locus_tag="IXF98_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1555220.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="IXF98_000889"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="IXF98_000889"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1555221.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="IXF98_000890"
     CDS             complement(14547..15245)
                     /locus_tag="IXF98_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1555222.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="IXF98_000891"
     CDS             complement(15202..16863)
                     /locus_tag="IXF98_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1555223.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="IXF98_000892"
     CDS             17081..18481
                     /locus_tag="IXF98_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1555224.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="IXF98_000893"
     CDS             complement(18733..19812)
                     /locus_tag="IXF98_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1555225.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="IXF98_000894"
     CDS             complement(19898..20857)
                     /locus_tag="IXF98_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1555226.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22392)
                     /locus_tag="IXF98_000895"
     CDS             complement(20869..22392)
                     /locus_tag="IXF98_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1555227.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22965)
                     /locus_tag="IXF98_000896"
     CDS             complement(22402..22965)
                     /locus_tag="IXF98_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1555228.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22971..23834)
                     /locus_tag="IXF98_000897"
     CDS             complement(22971..23834)
                     /locus_tag="IXF98_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555229.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23848..24744)
                     /locus_tag="IXF98_000898"
     CDS             complement(23848..24744)
                     /locus_tag="IXF98_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555230.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24919..26190)
                     /locus_tag="IXF98_000899"
     CDS             complement(24919..26190)
                     /locus_tag="IXF98_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555231.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26180..27697)
                     /locus_tag="IXF98_000900"
     CDS             complement(26180..27697)
                     /locus_tag="IXF98_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1555232.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27895..28785
                     /locus_tag="IXF98_000901"
     CDS             27895..28785
                     /locus_tag="IXF98_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1555233.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="IXF98_000902"
     CDS             complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="IXF98_000902"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1555234.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29694..31166)
                     /locus_tag="IXF98_000903"
     CDS             complement(29694..31166)
                     /locus_tag="IXF98_000903"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1555235.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31803..32165)
                     /locus_tag="IXF98_000904"
     CDS             complement(31803..32165)
                     /locus_tag="IXF98_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1555236.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32388..33140
                     /locus_tag="IXF98_000905"
     CDS             32388..33140
                     /locus_tag="IXF98_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1555237.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33280..34203)
                     /locus_tag="IXF98_000906"
     CDS             complement(33280..34203)
                     /locus_tag="IXF98_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1555238.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34218..34490)
                     /locus_tag="IXF98_000907"
     CDS             complement(34218..34490)
                     /locus_tag="IXF98_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555239.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10233 a         7214 c         5981 g        11244 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
    22861 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
    22921 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
    22981 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
    23041 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
    23101 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
    23161 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
    23221 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
    23281 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
    23341 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
    23401 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
    23461 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
    23521 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
    23581 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
    23641 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
    23701 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
    23761 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
    23821 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
    23881 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
    23941 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
    24001 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
    24061 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
    24121 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
    24181 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
    24241 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
    24301 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
    24361 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
    24421 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
    24481 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
    24541 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
    24601 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
    24661 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
    24721 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
    24781 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
    24841 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
    24901 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
    24961 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
    25021 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
    25081 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
    25141 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
    25201 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
    25261 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
    25321 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
    25381 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
    25441 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
    25501 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
    25561 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
    25621 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
    25681 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
    25741 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
    25801 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
    25861 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
    25921 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
    25981 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
    26041 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
    26101 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
    26161 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
    26221 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
    26281 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
    26341 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
    26401 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
    26461 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
    26521 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
    26581 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
    26641 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
    26701 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
    26761 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
    26821 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
    26881 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
    26941 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
    27001 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
    27061 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
    27121 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
    27181 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
    27241 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
    27301 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
    27361 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
    27421 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
    27481 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
    27541 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
    27601 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
    27661 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
    27721 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
    27781 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
    27841 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
    27901 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
    27961 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
    28021 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
    28081 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
    28141 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
    28201 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
    28261 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
    28321 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
    28381 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
    28441 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
    28501 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
    28561 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
    28621 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
    28681 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
    28741 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
    28801 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
    28861 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
    28921 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
    28981 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
    29041 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
    29101 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
    29161 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
    29221 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    29281 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    29341 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    29401 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    29461 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    29521 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    29581 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    29641 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    29701 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    29761 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    29821 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    29881 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    29941 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    30001 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    30061 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    30121 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    30181 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    30241 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    30301 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    30361 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    30421 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    30481 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    30541 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    30601 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    30661 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    30721 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    30781 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    30841 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    30901 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    30961 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    31021 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    31081 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    31141 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    31201 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    31261 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    31321 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    31381 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    31441 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    31501 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    31561 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    31621 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    31681 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    31741 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    31801 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    31861 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    31921 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    31981 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    32041 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    32101 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    32161 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    32221 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    32281 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    32341 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    32401 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    32461 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    32521 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    32581 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    32641 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    32701 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    32761 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    32821 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    32881 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    32941 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    33001 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    33061 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    33121 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    33181 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    33241 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    33301 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    33361 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    33421 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    33481 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    33541 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    33601 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    33661 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    33721 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    33781 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    33841 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    33901 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    33961 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    34021 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    34081 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    34141 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    34201 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    34261 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    34321 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    34381 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    34441 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    34501 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    34561 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    34621 attataatga tactgtaaat atatctttat tattagtgaa tggatattta ct
//
LOCUS       DACETN010000021        33749 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DACETN010000021 DACETN010000000
VERSION     DACETN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.022"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(55..1284)
                     /gene="rpsA"
                     /locus_tag="IXF98_000908"
     CDS             complement(55..1284)
                     /gene="rpsA"
                     /locus_tag="IXF98_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1555240.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1419..2099)
                     /locus_tag="IXF98_000909"
     CDS             complement(1419..2099)
                     /locus_tag="IXF98_000909"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1555241.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2148..2783)
                     /locus_tag="IXF98_000910"
     CDS             complement(2148..2783)
                     /locus_tag="IXF98_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1555242.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2849..4252)
                     /locus_tag="IXF98_000911"
     CDS             complement(2849..4252)
                     /locus_tag="IXF98_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1555243.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4239..5270)
                     /locus_tag="IXF98_000912"
     CDS             complement(4239..5270)
                     /locus_tag="IXF98_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555244.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5301..5522
                     /locus_tag="IXF98_000913"
     CDS             5301..5522
                     /locus_tag="IXF98_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1555245.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5687..6424)
                     /locus_tag="IXF98_000914"
     CDS             complement(5687..6424)
                     /locus_tag="IXF98_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1555246.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6577..7917)
                     /locus_tag="IXF98_000915"
     CDS             complement(6577..7917)
                     /locus_tag="IXF98_000915"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1555247.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8154..9677)
                     /locus_tag="IXF98_000916"
     CDS             complement(8154..9677)
                     /locus_tag="IXF98_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1555248.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(10055..10666)
                     /locus_tag="IXF98_000917"
     CDS             complement(10055..10666)
                     /locus_tag="IXF98_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1555249.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10789..10914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10996..11712)
                     /locus_tag="IXF98_000918"
     CDS             complement(10996..11712)
                     /locus_tag="IXF98_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1555250.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11718..12287)
                     /gene="scpB"
                     /locus_tag="IXF98_000919"
     CDS             complement(11718..12287)
                     /gene="scpB"
                     /locus_tag="IXF98_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1555251.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12271..13068)
                     /locus_tag="IXF98_000920"
     CDS             complement(12271..13068)
                     /locus_tag="IXF98_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1555252.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(13038..13409)
                     /locus_tag="IXF98_000921"
     CDS             complement(13038..13409)
                     /locus_tag="IXF98_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1555253.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13428..14315)
                     /gene="xerD"
                     /locus_tag="IXF98_000922"
     CDS             complement(13428..14315)
                     /gene="xerD"
                     /locus_tag="IXF98_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1555254.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(14411..14884)
                     /locus_tag="IXF98_000923"
     CDS             complement(14411..14884)
                     /locus_tag="IXF98_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1555255.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(14989..15852)
                     /locus_tag="IXF98_000924"
     CDS             complement(14989..15852)
                     /locus_tag="IXF98_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1555256.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(16048..18147)
                     /locus_tag="IXF98_000925"
     CDS             complement(16048..18147)
                     /locus_tag="IXF98_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1555257.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(18367..19476)
                     /gene="rpoD"
                     /locus_tag="IXF98_000926"
     CDS             complement(18367..19476)
                     /gene="rpoD"
                     /locus_tag="IXF98_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1555258.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(19500..21341)
                     /locus_tag="IXF98_000927"
     CDS             complement(19500..21341)
                     /locus_tag="IXF98_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1555259.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(21523..23916)
                     /locus_tag="IXF98_000928"
     CDS             complement(21523..23916)
                     /locus_tag="IXF98_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1555260.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(24023..24883)
                     /locus_tag="IXF98_000929"
     CDS             complement(24023..24883)
                     /locus_tag="IXF98_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1555261.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(24942..25574)
                     /locus_tag="IXF98_000930"
     CDS             complement(24942..25574)
                     /locus_tag="IXF98_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1555262.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            25783..26502
                     /locus_tag="IXF98_000931"
     CDS             25783..26502
                     /locus_tag="IXF98_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1555263.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            26528..29119
                     /gene="mprF"
                     /locus_tag="IXF98_000932"
     CDS             26528..29119
                     /gene="mprF"
                     /locus_tag="IXF98_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1555264.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(29307..30494)
                     /gene="tuf"
                     /locus_tag="IXF98_000933"
     CDS             complement(29307..30494)
                     /gene="tuf"
                     /locus_tag="IXF98_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1555265.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            30862..31218
                     /locus_tag="IXF98_000934"
     CDS             30862..31218
                     /locus_tag="IXF98_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1555266.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(31459..32463)
                     /locus_tag="IXF98_000935"
     CDS             complement(31459..32463)
                     /locus_tag="IXF98_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1555267.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            32629..33480
                     /locus_tag="IXF98_000936"
     CDS             32629..33480
                     /locus_tag="IXF98_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1555268.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            33497..33742
                     /locus_tag="IXF98_000937"
     CDS             33497..33742
                     /locus_tag="IXF98_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555269.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         9433 a         7113 c         5826 g        11377 t
ORIGIN      
        1 gaaaaactga gacaaaagca gctgctttca tctcagtcac agatacaaaa tcaattattt
       61 ttcagaatca tcggttgctt tttcttctac ttcttctttc aacgcatcac ctaaaatgtc
      121 tcccatagta aatccagtat tttcttctgg taattcataa gactcaacat tttttggttc
      181 ttcttctgaa gctggttttt cttctaaagc tttgatactc aatgcaatac gatgttcttc
      241 tggatggatc tctaatactt tcactttcac ttcgtcacct tcatgaagca cttcatgtgg
      301 tgtcgcaata tgtttatgtg aaatttgaga gatatggact agcccttcta ctccagggaa
      361 cacttcaaca aaggcaccga agcttgtcaa acgtttaact gttccgtcta atactgtgcc
      421 aactggagct tttgtatcga tttctgacca aggtccaggc tgtgtgtctt tgattgacaa
      481 ggaaacacgt tcttgttcag gattgataga caacactttg acatttacat catcattgac
      541 agccaataca tcacttggtt ttgctacatg actatgtgaa atctctgaga catggactag
      601 accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc
      661 actgagaaca tctccctcat ggatagttga aagaatttct tttttctttt cttctctttc
      721 gttttccact accgctttat gcgataagat caaacgattt tctgatggtt cgatttcgat
      781 gattttaaag ctcaatgttt tgcctttata gtctgaaaag tctgcaacaa aatgatcttc
      841 caccattgat gcagggacaa aaccacgtac accgacgtca acgactaaac cgcctttgac
      901 aacatttgta acaggtgctt cgatgacctt gccctcttgg aaatctcttt cgatatcttc
      961 ccaaactttt ttagcatcta aacggcgttt tgaaagtaga tagctgccgt tttctttatc
     1021 tttgccgatc gtactgataa cgactaaatc taatacatca ccgactttta ctacttcgct
     1081 gatatcttct accggcaaag ttgataattc ttttgccgga acgactcctt caactccagc
     1141 cccttcgatt ccgacaatca cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac
     1201 atcgccaact tttacttctt gtacgctttt cattgcgtct tccattgact ggttgttctc
     1261 ttgattttga tcaaattctg tcataacact tgtcctccta accaacattc tgccgtaagt
     1321 acggtactgt ttttagttta attgattatt caaaaaaaat aaagcgattt cgttcgtttt
     1381 ccttgaaaaa acaattgttt ttcatttttt ttcttaattt aaaaaagaag ataacctttt
     1441 ttcaaaacaa cagcttttat tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg
     1501 atacgaacag catcctctgc ttgcttcagc ggagagactt cccttgttga atcataatgg
     1561 tctctttcct gtatctcttt cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg
     1621 ttctctttga aacgtctttc tgcacgctca cttacgcttg ctactaaaaa gattttcact
     1681 tctgcattcg gtaatacagc tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt
     1741 tcccctattt ttcgttggac agcaactaat tcttctctga cttttccatg tgcagagact
     1801 tcagaaacag cttttgtgac atctggttga cggatttcta aagtcacatc ttttgatgct
     1861 atgaatacat gctgtccatc ttcttgttgc gaaaaagaga taggatactt tttgatcagc
     1921 gaaactagcc cttcttcatc tgaaaaagat acattgttcg taatagctaa ataagttact
     1981 gcgcgataca tggcaccggt atctgtataa atataattca attcttttgc taatatcttg
     2041 gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg
     2101 atataactcc tttttctcag aaaaaaagcc gcaaagcgcg gctattatta tttaattctt
     2161 aattcttgac cagggtaaag cataaagttg tttggatcta agccattcaa ttcaaacaat
     2221 tgatcagtgg tgatccctgc acgctcagcc acttgttttg gaccttcgcc tgcttgaaca
     2281 gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg tttctgctgc attgctgctt
     2341 gaatcccctg cttgataagt tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata
     2401 ctgctttctg acgttacacc tgtatcttga gacacagtag attcttccgc gattgtacta
     2461 gattctactg ttgaagtctc agttgactcc tcttttgttg tacttgtaga actttctact
     2521 actgatgtcg aagtttcaga agaagttgct gctgttgcat tgttgcttga tccatttctg
     2581 atccagaaat aagcacctgc tggaatagcg atacataaga ccagtaaaat caaaaatagc
     2641 gaaagaaata atgtatttcc tttcttttgt tgtctctgag aacttcttga tgaaacttca
     2701 tcatctgtat cgtagattgg ttgttcccat ggttcttgtg tttcgttatt ttctgtagat
     2761 tgatgtctat cttttctgct cactgtttta cctcctaaac tctatcgtgt gtattatacc
     2821 ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat gtttttttta aataaattta
     2881 gtaaaatcgt ttcccaatga agttgttcag tcttagaaaa aagcttcctc tttggcagaa
     2941 gttctgcttt tgctccatca atatcacaac aattctgcgg cttttcggag agtttttcgc
     3001 caaaataagc taagatgaat tttctgcggc agttttcttc attaatatag cgaagcatct
     3061 gctgcaggcg gaattctttt tctttttcct gacgtttgag ccgttccacc caattttctg
     3121 gtttttcaga ctgctggatc agctccatcc attttttttg cagctcatca aagggagctt
     3181 gctctgtggc atattccaaa taaagctcaa aactttgtcg ctgttctcta gaaagttgat
     3241 taaagaaata atggatacgt tcatccccac tttgataaaa aagaattgct gcactttctt
     3301 cctgatctct gcctgctcga ccgatttctt gtacatagtt ttctagactg tctggcaaat
     3361 cataatggac cacataacgg atatcagatt tatcgatccc cataccaaat gcattcgtag
     3421 caatgagaaa ctgcagctga ttttttacaa actgctgttg aagttgtctt ctttgagctg
     3481 catcaagacc accgtgatag tatccaactg taaaacgttc cctgaataaa tgatacaatc
     3541 gttcgacttc tttttttgtt gcacaataaa tgattgccgc tccttgtgct ctttccatga
     3601 agtgttcaag ttcttgttct ttttgctgcg ttttccgaac aaaaagcgag atattttgcc
     3661 gattgactga ttgtctgatc tcttttggtg gtcgatcaaa tagtacatga cgtatatctc
     3721 ttgctactaa atctgttgct gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct
     3781 ttcggatttt tcctaactgt tgatattctg gacggaaatc aactccccat tgagacacgc
     3841 aatgagcctc atctactaca tataacgcaa tattttgttt tttcagatgc tctaaaagag
     3901 aaggattagt caacatttcg ggactcaaaa acaaaaattt atattgcgat agatgacgga
     3961 ggacgtatct tctttcagat tgcgaaagta aactattgaa agcagctact cgtttctccc
     4021 ctcgtttttg taattgcgtc acttgatctt ccattagaga aagcagcggt gacacgatga
     4081 tgaccagacc ttccattaaa taccccgtca attgatagca aaggcttttt cccgttcctg
     4141 taggcaaaat agcgagagta tcttctccat caagcaacgc ctgaataatt tcttcttggc
     4201 cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc atttattaac attctgtatc
     4261 gcttcctttc tcaatagata gatttgatac aaacgaaatt ctccataatc taattgtcca
     4321 tctacattcc attcgctaaa gcgccactct cgcacggaat ccttatttgg ttccaaacgt
     4381 tcgattgttt ctgcagagag aatccactcg tatggaaaat catcaaaaaa caaggcccac
     4441 tcaataagat gatctgttac tgttcctctt tttaaatgac ggattgccat gatctggtca
     4501 attgtctgac cattcataaa catctgcttg gtgatcatca tgctttgatt gaaattttgc
     4561 tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg gccgtttttc gatttgtgct
     4621 aacaaaaggt ggatacatct gctttgttgc agataaaccg ctgactcatc attgtttttt
     4681 gccagctgat aaggaagcag ccctgttcgc tcatttccag ataattggtt agctaaaaaa
     4741 tctgctgctt cttctggtat ctccgaaaac agctgcacca aactctcata cgcagtatca
     4801 atcaaaattt cacgtggcaa ttggctttca ctaagccatt tttttagttg gaaagtataa
     4861 aaaggacttg tctctgctgg aagatagtct tgaatacccg ttgccagatt agaaattact
     4921 tgaacagcaa atttaatgag ccgccaacta gtttcccctg ttctgccata gcggtcaaaa
     4981 cgcaaaccag tatgttctat ttgatgttcg gacaacatat cagcccctgc aggcgttagc
     5041 tttgcttcat tttcatttat ttcgatccaa ccttgctgcg caattttttg caaaatttga
     5101 taaaacttat cctgcttcaa ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta
     5161 aatccataaa taagtactga agtcgttcgt tttcccacaa ggagttggta aagagaacta
     5221 atccgcagct tgtgtcctga tgaaaatagc gataaaataa aaacaatcat attattctcc
     5281 ttaagttagg atggttagaa atgtcactac tatgtaaact tgtacctgaa cgctgtattg
     5341 catgcggtct ttgtcaaatc gaagcgccag atattttcga ttatgatgaa gaaggaatcg
     5401 ttttatttgc ccaagaacca gaggctcatc aacagttcgt gatgccggaa gaagaaacat
     5461 atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat tttattagaa aataaaatat
     5521 aaatataaac gaaactttaa aaacgtacac taagttctct aaacttgaaa ataagccggg
     5581 atctgtttct cttgttccgt gaaatcccga cttattttga aagattcatt tgctatctat
     5641 agttaattga ctagtttttt cacggaccct ataggaccca tttatattat ttcaactttt
     5701 tcagccagtt ttccaatgta ttgacgatca cagtttgctg gtctgcgtta gaaatttctg
     5761 cctctccatc tcctttttgc tcaccatagc tgccaaaacc tccatgattt ccgccattga
     5821 ttgatacata ctctgttctt tccggtaaat actgcttagc ttcttgataa gcttcctgat
     5881 tcaatacttt atcttttgtc gccgtgatcg ataaaacagg aacagctgat tctgtcaatg
     5941 ttcctttttt gtcgggataa cttgccatga agaatacccc ttttaaatgt ttgtctgaat
     6001 gattcttatt gacataacga cttgccatta cgccacctaa tgaatggcca ccgatcacat
     6061 aatcggaatt tggaaaatct tctttgacct tatccgcttt gtttccgccc atcactgcta
     6121 gatcaagtgg cattttgaca atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc
     6181 aaatactata actttctggc tctacgagag caccaggata aaaaataagt aatggagact
     6241 ttccatgatt ctttttctct ccttcaaaaa ataagtaatc ttctttgttt tttccttctt
     6301 ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt ttgctgtata tagtaataac
     6361 cggctccacc gagtaatcct aaaatgatga ctaagccaat tagtattcgt ctaaatattt
     6421 tcactttctc ccaccttttc tatctgagtg accccttttt cttaatcaag tatgattata
     6481 ccttaaaaaa ataagagaga aatgcaaact ttccagcatc aaataaaaaa agacggaata
     6541 tccatttgaa tatccgccct ttttgtatgt ttatttttat ttagcacata aacgatacac
     6601 agcttctgca taaatttcca ttgctttata catactactt aattcccatc gttcattcgc
     6661 ctggtgcata aagtcaggag tatcagggaa catcgcaccg aatgccacac actggttcat
     6721 cgtacgagca aaagtagcgc cgcctgatac aaacggttca gtcatgtctc cagtaatatc
     6781 gcgatatgtc cccaatagat ttttcactaa ctcactgtct agtggcacgt ataatgaatc
     6841 aaggaagtca aattcttcat aagtcaaccc atatttttct gctgttttag ttaatttagc
     6901 tactaaatca tctttcttga atgtaacagg gattctcata tctacaccga tttttgtttg
     6961 ttctggtgtg atttccaaac ttgcaaagtt catcgttagc tcgccggatt gttcatcttc
     7021 tgttttgccc acgacagctt caccagttgc attttcttga actaattttc ctaagaaatt
     7081 gattgggcca aaatcaaata cttctgaaag agcaattgcc agacgagaaa tagcattcac
     7141 tccttgtgct gcatctttcg cgtggatact tttaccaaga acgacgatgc cttcaccttg
     7201 ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt acttcagaaa gcttttctcc
     7261 agagtaagga gctgcatcag gaacaacatt caacgctcct ccagctttta cagaaaactc
     7321 gttcgttcct ggtcctgtca agtaagcttg caataagcct ttttctgcat aaattaaagg
     7381 aaattcagca tctggcgcaa atccttgcgt aattccttct tctttttcgt tatatttttc
     7441 taaacaacgc cataaatttt cttcatctgt accaaagata aaacgaatac gtgtcttgaa
     7501 ttccacgcca gcatcaatca aagctttgac ggcatacatt gccgcaatag aaggaccttt
     7561 gtcatcttgg gatccgcgac caactaacca gccgtctttt atagttggat cgaatggttt
     7621 tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc atatgacaca agataccgaa
     7681 taattcatct ccagagccaa tctctgaata accgtaatag ccttctggat ctttaaatgt
     7741 tctaaatcct agcttttcac tgatttccaa tactttatcc aaagcttcaa tcactttttt
     7801 accgaaggga tgtccttgtc ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa
     7861 ttcgctcaat gattctaatg cttgttcatg atgttgttct gtcacaaatt ttttcatgat
     7921 attatcctcc taatatgtta aaaagggtca tgtcaaattc ctccgacaca acctcttctt
     7981 ctcaaataat gacgttttaa gcctgctcca tcagcaagca gaatgttctt aacaaatgat
     8041 gaaagaaaaa acactcactg aatggagcta ttgacttatc cacgtgctta tcgatagccg
     8101 tttatcggtt tcttcattct tatgatagac cttgtacctg tatttgtcta ggtttagaac
     8161 attgctgcta cacctaggat cacacagatg gctacaaata atatcgcaat taatttaccg
     8221 gtaaatttcc accacactga aacatcgaaa cgagcaattg ctacagcacc cattacgata
     8281 gcagatgttg gtgtaattaa gtttactaaa ccagatgcag attgataagc agtgattact
     8341 aaatccttac cgacgccggc aaattcaccc ataggtccca tgattcccat tgttgcagca
     8401 gctaagccgg aagttgaagg aatcaagaat gacataggga tatagaaaat aaatgtaata
     8461 atgatgaata cacttgaaga taatgaactt aatccttgtt cgccccaatg taagatggtg
     8521 tctgtgatta atccattgtt cataaccact tggataccac gagcaacagc tactacgatt
     8581 gctacaccga tcaaatcaga agcaccagct aaaaattcag agacaaattc agactctttc
     8641 atgccataag tgattgctac tacgatggac attactaaga ataacatagt aatttctggg
     8701 aagtaccagt cacctaatgg aagcatgctt ttacctagaa ccgtacctag taccggaagg
     8761 tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa
     8821 ggaatcaaac tgatgatcat gataccaaaa gtaataaaga acaatacatt aacatgtttt
     8881 tgttttttcg tgattacttc ttgacgaccg attgcttcaa tatcgaaatg cttcatatct
     8941 tctttacgtt ggttgtatac taatgatttt gttggatctt tttcgatctt tgatgcataa
     9001 cgatacacat atacaataga aatagctaac aaaataacga acatcaagaa tcgaagacca
     9061 ataccttcac ccatcgaaat cccaacagct tgtgaagcta cgcctgttgc aaaaggattt
     9121 accgttgaag ctagacagcc tacttgagac cctactaata cgattgctac agctactact
     9181 gtatcaaagc ctacagccat catgactggg atcagtaaag catagaatgg aatcgtttct
     9241 tctgccattc cgtaagtaga gccgcctaat gcaaataaca gcatcaatac tggaataagc
     9301 attttttctt ttcctttgaa tttcttcaca actgaagcaa tcccggcatc tagtgcccct
     9361 gttttgttga taatacctaa aaatccacca acaaccaaaa tgaagaaaga gattgggatt
     9421 gcagcaggtg tttctttagt tagctccccc gctggtacac ctagcatccc attgattggc
     9481 gccatgaaaa tatcccacag cccttgagga ttttgttgta ctcgttcata tgatccagcg
     9541 acgatgttgc ctgcatcgtc tacactatag tgcccagctg gtataaacca agtaagtaca
     9601 gcgattactg caataataat aaataagacc gtatacgctg aaggcatttg aaattttttc
     9661 ttttttgttt gttccatctt cttcttcctt tcttttccgt tagagacaac aattttggct
     9721 ctaatgttat tttatcaaaa agagtatcag aacaaaaata ataacacgca ttttgtaaaa
     9781 cttttgttca aaaagttcgt ttttccctat tccctcctct tttttcaggt ttttaaagct
     9841 ttcttctttt tcattgtagc gtttcctttt ttttataaac aaacattttc gtcttttaaa
     9901 aggagaagaa tttcacattt gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat
     9961 actcaaaaca aacaaataaa acgtttacat tattgcatgt cttgtatttt tttccaaaaa
    10021 aaaagaccaa gaaatttctt gatctttttg atatttattt gactgctgta tgacgttgtt
    10081 cgaactgatg ttgttttcga cttaaccatg gaagaagttg cgcatgcagc actaaaaaga
    10141 cagcactcac gatgaatcct ttgatcaagt tgaaaggaac gatcccaatc agtacatagt
    10201 ttgctaaaga catgcccagc atctgtgttg cgttcagccc taaaaacatt aagtaaagag
    10261 gcgtaataac gaaataattt gcgatactca taaaaatagt tagagcgatg gttcctgtaa
    10321 tgacacctaa aaatttgttt cttcctttat gctgtttctt tacactaaag aaataataaa
    10381 tcgggaatgt aaagatcgtt gttgataaga aactagcagt gtctcctacc aaattatccg
    10441 gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg
    10501 ccgctggacc gaataagaac atgctgatca ataccgagat atcactaaaa tcgatcttca
    10561 aaaaactaaa agccggcatg atcggaaaag caaaatactg taaaactaat gcgatcgcag
    10621 caaacatcgc tactgcaacc attttctgta cacgggtgtt cttcatttga aatcctcctg
    10681 ttaggactcc tcttcagcga actttttgac gtttctctcc aaatgaaaaa actctatttt
    10741 ccagggaaaa tagagttaga gttatttgat caagcgtcgt caaataagct ctatcttctt
    10801 tatccagact atactgtcgg tatcggaatt tcaccgattc agctattttt ttgcaaaata
    10861 ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc ccctgaagac gaaccttata
    10921 taatttttgt ttacatattc attatacata tctattcaaa aaacgcaaca tctaacactt
    10981 ataaaaagta agtgttcatt ttgagtggtt cagcaaactg ctgacttctt ttttgttcag
    11041 ttcacggtaa tccccaggac gtaggccttg taaagtcaga tctccgtatt tttcacgttt
    11101 tagtttttgc acaggaagac caactgcttg aagcatattt ttcacttgat gattacgtcc
    11161 ttcgtggatc gtcaattcca cgatactgtg gtttgtctta ggatccactg ataaaatttg
    11221 gaatttagct ggagatgttt ttcgtccttc gatctttatt ccttttgtca aaggtgcaag
    11281 catcgatttt gtagcgatcc cttttacctt tgcaacgtaa actttatcca cttcatgttt
    11341 tggatgtgtc aggcgctgag caaaatctcc atcatttgtc agtagcaaga tccccgaagt
    11401 atcgtaatcc agtcgaccta caggatagat acgttctttt accatcggca aaagatccgt
    11461 aacaactttt cttcctttgt catctgcaac agcagaaata actcctttag gtttgtacaa
    11521 taaatagtaa ccgtattctt cttgataaat tgggacttca tccacttcca cagtgtcatg
    11581 tttcccaact tttatcccta attcccttac aacttgtccg tttactttta cacgtcctgc
    11641 aaggatgaat tcttctgctt ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg
    11701 caatctctcc attacaatta ttctccttta ttcattccat caaagaatag atctggtgac
    11761 acgtcttctt caagtgaaat ttccatttct tgtgtatcag gcagttcttc taatgatttc
    11821 aaaccaaaat aatctaagaa gtagccagtt gttccatata atatcggccg acctggtcct
    11881 tctactctac ctttttcttc aatcagttta tgggcagcta gacgctgtac cgctcctgaa
    11941 acctgaactc ctcgaatctg ctcgatttca gctcttgtga ttggctgttt ataagcgatg
    12001 attgctaagg tttcgacggc ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc
    12061 ttaagtaagg gagccagctc ttttttagtc gttaatacat aatggctttc cgtttcaaaa
    12121 atatgcaaag ctgagtgcga gttttgagtg taagattctt ttaattcttt cactaaactt
    12181 gtgacttctg cttcgctgag atttaacagt ccgcttaatt cttcattact gatcccttcc
    12241 tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc
    12301 tactgctgga taaagcataa ttgattcata gttttctttt tgttgcgcaa tgatctgccc
    12361 ttttttcatc aattctagca aagccataaa agtagtgaca acttcacttt tagaaggtga
    12421 ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt tttatacgct cttcgataaa
    12481 taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga
    12541 aaatttctta cgttctaaga ttttctgcat ggcacggatc aaatcgattt ttttgacttg
    12601 tcctcgcttc aacggctttt cagcatccgc taacccatcg atgttcatag gttcttttgt
    12661 gtaataaaga cttcgttcct ctgctttttc tgtcagtctt tcagctgcat atttatattt
    12721 acggtattct aaaagctgag tgaccaattc ttctcgagga tcttcttccc ataattcttc
    12781 atcgtctgtg acgaattctt ggacaggaag caatgtcttg cttttgattg ccatcaatgt
    12841 agcagccatc actaagtatt ctcctgcaac agctagctcg agtgttttca ttgtatggat
    12901 ataattcata tattgctccg tgatcgtagc gatcggtata tcatatatat cgatttccat
    12961 ttgctgaatc agatgaagca gcagatcgag aggaccctcg aatacatcta atttaatatt
    13021 gatctccgtc aactcattca ctctttcttc tttttctttg ctagccattt tccaatgtaa
    13081 ggctctgtga tttcagttgc acggatggag acatccgggt acttttcctg taattcatct
    13141 aacatttgaa agcccatatt ctcaccgcga taagacggat tcaaagaaat gtcatggatg
    13201 atcagctgat cgttcgttgc ccagtaaata ccaataatcc cttgaatgtt ggttgaacct
    13261 tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt cgtacgtatc aatctccttc
    13321 aacaaagaag ttttacctgt cagtttctta tggaaactca atagtcccat agcaatttta
    13381 cggttttctt tccggtaatg tgttaacatc ttctcacctt cctatgacta agcccttgga
    13441 aaatgctgct tataaacatc cgtcattctt ttcttcgtga tatgtgtata aatctgagtc
    13501 gtagagatat ccgcgtgacc taacaattcc tgtactgtcc gcaaatcagc tccattttct
    13561 agcagatgtg tggcaaagct atgccgcagc gtatgtggtg ttacttcttt gtaaatccca
    13621 gcctctcgta ctaactgctt cagatttttc cagattcctt gccgagaaag acctttccca
    13681 tgattattga caaatacatg catttcagat gcgtccttta caagtaatgg acgtgcttcg
    13741 tctaagtagc gttccagcca ttggatcgca taatcaccta aaggaatgat ccgctcttta
    13801 tctccttttc caactgtctg taccagtcca agagaaagat gaagatctcc caatttcaat
    13861 ccaatcaatt cactgacgcg catcccagta gcatacatga cttccaagat tgcacgatct
    13921 cttatcccta acgtttttgt cgtatcaggt gcttcaatca gcttttctac ttctgtcaga
    13981 gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat cgatatgttg catcggatca
    14041 tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc ttctgagact tgagatcatc
    14101 cgagtaacag ttgctgtcgc ttttttttct tcatgcaact tctccagaaa acggatgact
    14161 aaaaagcgat cgacatcctg ccatgaagca attttctgct cctgtagaaa atgcaagtac
    14221 tgttccaaat cacgttcata gctttgtcgt gtattggcgg acaatccccg ttcaatattc
    14281 aagtagtgga gataatcgtt gatttgctct ttcataataa tccccctcat gcctctctat
    14341 catatcagat accgcttcat tctgatagtc tttttccata atctgagcaa agaaaaagcc
    14401 gtctagacac tcagacggac tggtttcttt tttctttttc atagcattcc tgacagattc
    14461 catggaacgt tagccgatga tcttttacta aaaaatgata acgttcttct actacttgct
    14521 ccacttcaag taaaagatcc tcttctactt cctcgatcgt gccgcagttc aagcatagta
    14581 aatgatgatg gaaatgtttg gcgccttctt ttctcaaatc ataacgggct aatccatcat
    14641 taaaacttac tttatcgaca attttcaatt ctgtcaagat ttcaagggtt cgataaacag
    14701 tagcaagtcc aatttccgga ctcttttttt tcactaaaaa gaagatttct tctgcagata
    14761 aatgctcttt ctcattttcc aaaagcacaa gcagagtagc ttctctttgc ggtgtcagct
    14821 taaaccccgc ttcatgtagc tgctgttttg tttttttcat cgccgaaata gcattcatga
    14881 ccatttactc aaccccaaca atttataata attacaaata aaaaaagata taacactatg
    14941 ttaattaaaa ttattataag ctagttaaga aaaacatgca agggattctc aattagattt
    15001 tttgactagt tttgtcacac cgttttcttg tgtaatcaat ccttgtttca aaagattacc
    15061 taatgcgcgc ttaaatgctc ctttgctgat accaaaagct ttcatgattt cttctggatc
    15121 cgatttatct gtaaagttga tttgctgatc tgcagaacgt tctaaaatgg ttaaaatcat
    15181 ttgtgcatca tctgatatag cttcatatgc tcttggcttc aatgaaagat tcagtacacc
    15241 gtctggtcgg acaccaatca ctcgaccttt tatctgctcg cctagacgag gttcattata
    15301 tcgttcagaa ggatggataa acccaagata gaaatcttcc gttaaaacgt acgtacctgt
    15361 taatttcaag cggtatgctg ttcctatcac atctttgttt ttcatttctt ctgttccata
    15421 tttgcttaaa gatttgaata ttttttcatc tgctaaactg ccccaaatac ggtcttttgc
    15481 atcaactcta agggaaatca tcaagcgatc ccctttttta ggccatagct ctgtcattgt
    15541 cggcaattca tcaagagata cgacaaagtc tttatctgga agaccgatat cgacaaacgc
    15601 acctaaatct tttcgtgtag cagtaacgat tccaaaagcg taatgaccga tacggctttt
    15661 aggaatttgt gtagtgatcc gattttcatg tttttgattt tggtaaccga atccttcaac
    15721 tgcttcaccg attttgtgtt caccttctga tttgcttaag ccaaaggttt gtccgttttt
    15781 ttggacaaag tagttttttt cattttcatc aataataata cctgtaaata tagttgctaa
    15841 taaatcgttc atttttttct ccctctatag tgaaaggttt tcttaataca tttctttgct
    15901 atcataacat aatgttatct acttgaatag ctgagccttg gtttggaaag gtgaaaaaag
    15961 gagtatgaca acagttgtgt catactcccc tttttcgaat aaacggtgtt aaacggcagg
    16021 ctcacagcct ctttcatttg cctaaaatta attgctccca tttcctccga accatccagt
    16081 tttacagttg atggttacga tgtaaaatgg caaagcactt tctccaatgc ctttcatcaa
    16141 tgcagatttt gttgaagtgg catatagatt ataatagcct tccccatttt cgattctggc
    16201 tttttctaat ctgtaacctc cttcaacgat atatccacgt tggatacatg tagcaatcac
    16261 tttgctttgg acacctggtg cccacgtcca tgctggatta gatgcatcgg caatatcact
    16321 tgctctggca gatttgattg gctgattcgc tgagtttgaa gaggaagggg tcgaggatgc
    16381 cgcttcttgc tgtgctgctt gtgcgaccgc agcttgttga gcggcttgag ctgcagctgc
    16441 tgcttgagcg gcttgttttg cttgttcttc agctgctttt ttcgcttctt ctgctttttt
    16501 cttttcctct tcttcttttt tctctttatc agtgagcatc ttatctactt gtttcaattg
    16561 ttcattcaac tgagcagata gatctgtatt tttcactttt tctactgctt cttttgccgt
    16621 cttgtattgg tcgcgtgttg ctgaatccac tacttttccg tctttaaaga ctatagctgt
    16681 tttttcttta gctgtttgca attcagcata ttgattttta gcttctgttt gtgcttgttg
    16741 aatcaatttt ccaaaatctg tgttatccgt ttcgattgct tggatctctt tatctgcttt
    16801 caacttgatg tcatctgcta gatgatcatc gacgatggca ggtgctatga accactcatt
    16861 cactttttgg atatctcgga tcttagattg gatatcttgg taagttgttt ctaactctga
    16921 atatcctttt tcattcttaa gactggataa atcatttttt agttttgata aatcttgatg
    16981 aatcatatct gcacgaataa attgatggtg atcatctgta taaaaggcag caagtttctc
    17041 atgaagttct tccatttgtt gctgtctttt tgcttctgct gctacttcgg cttctacttt
    17101 ttgatgatgg tcataataag cccagcttcc tccaccgagt agaagtactg ctgctaaaac
    17161 gagataaggt actttttttg attttttctt ttgcgcttta tctggatctg ttttttctaa
    17221 gtttgattct tgatgtgttt tttcagtatc tggcacgatt gttattgatt tcgcttctgc
    17281 ttcaaacagc ttaggctggt tctcttcttt ttctattttt gcttcaagat cagtaccctc
    17341 tttgcttaca gattgatttt cagcatcgga ttcactattt atttcatcgg gctctgttga
    17401 ctttttgtct gtgttttcga gtatttcttt tttctctgct tccggcagtt tattcgtttt
    17461 ttccttttct aattgttttt cagtaaatac ttcagttttt tctgtgatat ccgtttttac
    17521 gtcttttatt tttactgttt ctttacctgt ggcgttttct ctatcagagt ctttttcagt
    17581 ttcttgttcc ttttctggct ctactttgtt ttcctgtgca attttttcac tgatcatttt
    17641 gagcagctct ttcgcgtgct cttcttttgt atcttcacga tgtgctttga tataagcgga
    17701 tagtatcgaa ttttcttcct cattatcgac gatttctttc agttcagcta ctcttctttt
    17761 ttgttttgat tcaagcgttt cttcttgatt ctttcctgat tcttccgaag tctcatgaac
    17821 agttgctttc ttctgttcat ttttctctat attagttact tgatcgtgag caggtggttc
    17881 ttcgtcttct ggttctgctt tttcgaataa ttccattaca gattctagag gtaaatcttt
    17941 caattccgac cattcgattg gatcgtttaa ttctgtatca ggaaagactg catcttcagt
    18001 tgatttatcc tgcatagctg catctgtatt tgtttcttca attgattctt ttatttgctg
    18061 atcttctgca gttatttgat gaccgcagtc tggacagata gtattctgct ccattttttt
    18121 cgatccgcaa tttggacatt ttttcatcac ttagcgatcg gctcctttac taggtttacg
    18181 ctaccattta atctatcata ttctatacgt cacgccaata gcttattttt aggatttact
    18241 taaaaataat gaatgaattt ccttcctata gaaaaaaaag cttgagactg cgtagtctca
    18301 agcttttttt cttttaaaat aaaccaaaca gttttactga tttctcttct ggtcaatagt
    18361 ccacgcttat tctaagaaat cttttaattg ttttgaacga gaaggatggc gcaattttct
    18421 taatgcttta gcctcgatct gacgaatacg ttcccgagta acaccaaata ctttccctac
    18481 ttcttccaat gttctcgtcc ggccatcatc taaaccaaaa cgtaagcgaa ggacattctc
    18541 ttcccgatca gtcaatgtat ctagtacatc ttccaattgt tcttttagta actcataagc
    18601 tgcgtgttct gctggacttg ttgcttcctg gtcttctatg aaatcaccta agtgagagtc
    18661 atcttcttca ccgattggcg tttcaaggga aacaggttct tgcgcgattt tcaagatttc
    18721 tcggactttt tctgttggaa gatccatttc cgcaccgatt tcttctggcg taggttctct
    18781 tccaaggtct tgcagcaatt gacgttggat acgtatcaat ttattaattg tttcaaccat
    18841 atgaactgga atacggatcg tacgtgcttg gtcagcgatt gctcgagtga tggcttgacg
    18901 aatccaccat gtagcatagg ttgaaaactt aaatcctttg cggtagtcaa atttttcaac
    18961 tgccttcatc agtcccatgt ttccttcttg gatcaagtca aggaactgca tgcctcgtcc
    19021 tacataacgt ttagcgatac ttactactaa acgaaggttt gcttctgcca gacgctgttt
    19081 cgcttcttgg tcgccttctt cgattttcaa tgctaacgct acttcttctt ccgcagttaa
    19141 aagggagaca cggccaattt ctttcagata catacgaacc gggtcattga tttttacacc
    19201 ggttggcgca gaaagatctt ctgtttttgc ttgttcagct tttttctcag cactcttcaa
    19261 gctgtgaatt gatggatcac cattttcatc caccacactg atacctgcgt cttcaatctt
    19321 ttggatcaat ttgtccattt catctgcatt caaagtgaat ggtgtagcaa gctgattgga
    19381 taaatcatca tatacaactt gtcgtttggg tttgttttct ttgatgaaag ctgctactgc
    19441 tgcttcatac gtttttttat ctgttccatt tgccataaaa gaaggccccc cttgagtagc
    19501 tatttggctt gttttaactg tttcgttaaa ttgatgattt caatcgtcaa ttcaagctca
    19561 agctgtttgt ttccagttcg tctggcttct tgctgcattt gtttttttaa tgttatttca
    19621 tcagctaagc cagatttgct gatcacatct agcaagtctt ggatttctcg tggcgaactt
    19681 tcttctgcca ccggtaaata ttcgatttcc accaccaacc gccttaaatt ttcatcttgc
    19741 aaaaagtcta aaaactctgc aaggttgaat tcataatgtt gtgagacata tgcatcgaat
    19801 aaaaaataaa cttctgcata tgcatcatga ataaactgaa attctcgttc ttttaaaata
    19861 ttacggacag aaacttcttt aaatgcacga tataatagaa tctgctctgc cttttctact
    19921 tgcgtcaaag gtctcttttt ccgtgcagtt tcttgtctcg taacctgagt gtgttgactt
    19981 tcctgaggtt cttgacgatt ttgctgattt gtccttcgaa gatcgcgcag ttgtttttgt
    20041 atcgtctctc tgccgatctg aaactctgtt gacagttgag atagatacat atcttgttcg
    20101 attggtgaaa caactcggct aagttcaact agtaattctt cgaggtattc gatcttgtct
    20161 ttttcattat ctaaattttt cccttgtttt aaataacgtg atttaaaagt aaagactgtt
    20221 tcgcgcccat gagccactaa ttcttggaaa gatgcttctc catacttacg gagatattca
    20281 tcaggatcaa gcttttctgg aacgactacg acagaaagtg ctaatcgact gttttgccga
    20341 agcaattcaa tccctcgatt ggttgcttca aaacctgcgt tgtctccgtc atagcaaaag
    20401 accaattctt tcgtcagctt ttccatttgt tggatctgtt gagctgttag acttgtcccc
    20461 atagaagcta atccagtttt gataccagcc tgccatgcag caatcacatc cataaaccct
    20521 tcgaacaaca aaatttctcc gtttttccgg atatcgcttc ttgctttatc aagattaaat
    20581 aaaacttgtc ttttattgaa cagttctgtt tctggactat tcaagtattt gggctgatct
    20641 ttttcctgcc ctttttcagg tgcaagccag cgtcctgaaa aaccgatcgt ctggccttga
    20701 gaattacgaa gtggaaacat cactctttga taaaaacgat cagacatacg gccatcatca
    20761 tgcgtgacaa acaatccaga gcgttctaac aattcatttg aaattgattc attcttgaat
    20821 acttgctgaa gaaaagaacg ctcattaggt gcaaaaccta tgttgaattc ttcaattaat
    20881 tctaaagaca acccacgact ttgcaaatat tctaaagctg gctgtccagc ttttgtatgc
    20941 aatagcatgt gatgatagat ttccgctgct ttttcatgag cagcaatcag tttccccgta
    21001 gcagattcag gattttggac agctatagta cgctgacgcc attgatcttc taacggtatc
    21061 tgttcgaagt ctgccacttt taccactgct tctggaaaac ttagaccttc tagttcttga
    21121 agaaaagtaa acacgtttcc gcctttacca caaccaaagc aatgaaaaat ctgcttgtct
    21181 tctgcaacag aaaaagacgg tgtacgttct tcatgaaaag gacacagccc caagtaattt
    21241 ttaccagact ttttcaactg gacatattgt ccaatcacct caacgatatt cgtttgactg
    21301 cgtactgttt cgataacctc ttgaggaatc cgttgagcca ttttttcacc ccctgctcta
    21361 ttgatatctt ttgggtttaa cagaagatat ttcaggagaa taaccggtaa aaaatcgcac
    21421 taactgaaat cagtgcgatt ttaaaggaca caattataca tgtttgattg tatcatattt
    21481 cattgctttt tcaagtataa cacatttcta tctcattaag catcaatttg ctactctttt
    21541 cagtaactga ttttgaatat cagccttcca gtacaatact cgaagaacaa tatttacgag
    21601 catgaataca aagaagtatc ctacgctctc tcccaaccct aagtttactt ccaaaattcc
    21661 atggaacagc attgcagcta catatatccc tacgatcgtc gttactgcta ggaagatgcg
    21721 caatgggttg aagggataac aagcttttac aactgccatg caactgatcc caatcagcaa
    21781 gtaatacatc aaagtagtag tttcagcatt tccccatgcg aatgtttttc caagtagata
    21841 caccgcaatg atattcacta ctacaagcaa ggcatttggt aaagcattaa taagtgctgt
    21901 aggcaaataa cggtaagtga tttttctttt atcttgttca aaagataaaa agaaggaagg
    21961 atacccttca atcgctaaat caatcagcgt gatttggata gggataaacg gaaatccaat
    22021 tgctgtaacg gcacatataa tcgataaaat aaatgaataa atcgttttga taaaaaagat
    22081 gcctgagact ttcgtcacat tgttgaccac tcggcgtcct tcaaacagga cttctggcaa
    22141 tgtagtaaaa tcagagtcca acaataccag attcgcaatc tgacgtgttg ctccatcacc
    22201 ttctgccata gcaatgctgc aatctgcttc tcttaacgcc aaaacatcat tcacaccgtc
    22261 gcctgtcatt gcaaccgttc tgccgctttc tttcaattca tttaccagca atttcttttg
    22321 ctgcggactc actcgtccaa aaaccgtata gcgatgagca gcttctcgca cttccatttc
    22381 ttcagtaatt tgagaaagat cgacatacgc atcataattc ggtaatcctg cacgtctagc
    22441 gatattggag acagtaaaag gattgtctcc agaaatcact ttcagatcca caccttcctc
    22501 atgaagataa gctagtgttt cttgagcgtt ttttcggatt ggatcatcga tttcaaacag
    22561 agcaaccggc tcaacaaatg ctggtttttc ttcgttcaat tgctgatttg gagcgatccc
    22621 caacataagt acgcgatagc cattcgcttg ggctgttttt agactgtccg gaagccgatc
    22681 ttcttccacc aaacgttcag gcgctcctac gtacacgact cccagttctg gaaattccat
    22741 cgcgccccat ttgcgatcag aagaaaatgc gactttgttt gtcacatcat accgattaga
    22801 aagctcaaaa taatctctca tagcccgcat agtgatgttg ttatcactgc tttctgccag
    22861 atacgtcccc ataatttctg ggatggcttt ctcgtacagc ggatgtagtg cttctacccg
    22921 ctggatactc attttccctt cagtaatggt ccctgtttta tccaagcata atgtatcgac
    22981 atgggccagc gtttcaattg aatacatgtc ttgaacaagg attcgttttt ttgccagttt
    23041 ggttacccct gtcgttaatg caatacttat tagcaatacc aaaccctttg gaagcatgcc
    23101 taacaaagct gctgcagatg caataacaga aattttgata gaagcatcgc ggatgaacag
    23161 tgcttccaaa aatagtatga tccccaaagg aataatcacg aaactagtga atttggatac
    23221 tttgcgaatc gaatctacaa gttcagaacg aatagctttg tgagtctttg cctcgctagt
    23281 caagcggaca gcataattat ctgcacctac tcggataact tcagccatga cagaaccgct
    23341 tgtcaaaaag cttccagaaa gcaattcagc tccagtttct ttgacaatca aatcagattc
    23401 gcctgtcaaa agagcttcat ttgcttctgc ccgtccagat ataacctgta aatcggctgg
    23461 tatctgttct cctgctccga tttttaccaa atctcctaat accagctctt tagtagaaat
    23521 cgattgttct ttaccgtccc ggatcactag tacttctgat tgggaaacaa tcgataattt
    23581 ccgtacaaga tttcttgcat gtatttcttg ataaattcca ataacgatat tgacgacaat
    23641 aattgctaaa tacgccatgt tggaatacgc acctacaaat aacagacaaa tcccaatacc
    23701 aaagttcaga aaattgaata acgtcaatat attatcgctg aatatttgtt ttgtactttt
    23761 tgatgcatct tcctggtagt cattttgctg gccagaagcc attttctgaa ctacctcttg
    23821 ctgtgtaagt ccattcattt tctttttctc cattttccgc caactttcaa aaataatcac
    23881 gtgtttaagt ctagcgtgtt ccaagattaa aaacaacttg tttttataaa atttatagaa
    23941 aaacacacga atgattttac aaaaaagatg ccaacctttt tgtttgaatc acccgtgtgc
    24001 tgtttttatt atcaatctat ctttacttat attgttcctc caaacggctc atccaccaac
    24061 ctagtgctgt accggctata aaaagaacag gacaagctag taccccagca actcccaatc
    24121 ccgcgtaatc ggtaggaatg atcataatcg ttgaagcaaa aactacacct aaaatgaaat
    24181 ggaatagctt gctatatgcc tttgagaaaa tgtaatccat gatttttgac aatccaagaa
    24241 cacaaagaat cccaccaatt ccgatcggca caatcacttc aagtcgtact tctttaaatc
    24301 cgtcagccat cgctttatac aatcccatat acaccaaaaa attagaaggg ctcaatcctg
    24361 ggacgatcat acccaaacca atcaatccgc cggcaatcag ccatgttcca aaattctgat
    24421 cgacttgccc gtcaaataat tgagcgccgt ataataaaaa caaataagcc agaacaaaag
    24481 taacaaccat gatgatcaca tcagaagtat ctcttccttt tttccctgct tctttccata
    24541 acgcaggaac agttccagcg atacatccga caaaaaacca taatatcgtt gtttcatacg
    24601 cacctaataa aaaactgact aaaaaagaaa gtagaaagat gcctgtcact ccgcctagac
    24661 caacaggcag aaaaaacaaa acattttctt tgaaattttt tgtaaggtgg gctaaaaaag
    24721 aaatcagccg ctcatacatg ccaaagacag ctgctaatgc accaccgctg actcctggca
    24781 agataaatcc tgaaccgata aacattccct tcacaaaccg taacatccag tctttggttg
    24841 tgtaccccac ttcttgattt tccatttttt gccttttttc cactatttgc gcactccttc
    24901 tttcatttaa ttttattcat gattgaacat ttcctgctat tttacttaaa gactttttca
    24961 taggtctcta ttattttttc attcattttg acaatatcag gataaaacat gttacctcca
    25021 ttatgataga gatagccgcc attgtaaagc aaggactgga tccgccaata tcggtattgt
    25081 ttcccttcct catttcctaa aagtgggctc agcacatctt tagaatacgc ttctgctaat
    25141 tctactgtgt tttctccgcc atgatttttt acataggcaa tataatcttt cccaaaatta
    25201 tatgcttgga cagctgtcca taaatcgcat ccttgtttct ttgcttctac tatcatttcc
    25261 gccaaatatt tgacaccttg atcaatactg tcagctggat tatcaatgat ctcagcttct
    25321 ccatgcagac tttccgagct ctgcatgaga tctgtccctt ttccttttga ttcggtcaat
    25381 attatcgcct tgatcaaatt ctcgtagttt tccatatgat aattttttgc agctgttgtt
    25441 atttctattt gatagctttc cactcgtttt accgaacgat aagttcttcc tcctaaaaac
    25501 aacaccgcac caataaccgt cagaatgatc atgtaaaaaa gaatacggat aatagataat
    25561 cgttttcgct tcatttcatc acctagttat tttgacatct ttttttatct tactatttct
    25621 ttatcttctt ggctatttct tccttgattt ctgtgtacgc cttaagaaaa aatgaatttt
    25681 cctgtatttt ttgacatctt acgaaaatga tcgttgtttt tcagtaaaaa caattgccca
    25741 atgactgata taatcgttat actttacaca gtaggtgatt atgtgaaaaa agaaaagaaa
    25801 tatgcaaaaa tgccagatgg gagcgaaatt tactatgaaa agagcggtca aggcttccct
    25861 ctctttttac tgcatggcaa tgacggcagc ggccgctttt tttcagaaca ggtccctgta
    25921 ctcgaacgct actataccgt gtatctagta gatagccgag gacatggtcg ttcgacaaac
    25981 gaagcatcta tgttgaattt tcagttaatg gccgaggatc tgaatacgat catgttgctg
    26041 gaaaaaatcg atcaggcaga ttttcttgga tttagtgatg gagcaaatct agcactggta
    26101 tttgccagca gcttccctaa aaaagttcat cgattgattt taaattctgg aaacacgctt
    26161 gttaaaggcg ttcgtttttc tgccagagtc atcagtaata ttcattatgc atgggtatgg
    26221 ttactctctt tatttcgccc aagtcttcgc aaaaatcttt tagttatcaa acttttgctg
    26281 catgacatcg ggcttacaga aaatgattta aaaaaaatca actcccccac tttgatcatc
    26341 gtgggaaaaa aagatgtaat caagctaaaa cattctttgt atatagctaa aactatacca
    26401 aaagcttcct ttgtgcttgt gaaagaacaa ggacatgaat tagctagaaa agatcctgaa
    26461 cgcttcaatc gagaagttct tcaatttctt tctgaaacat aactattagg aatagaaggt
    26521 gatttatttg ttaaaaaata ccatacaatg gctgaaaaac cacctaggac tgttcaaaac
    26581 aatctttttg atttctgtca ttgtcattat tgtcggtgaa ttgatgtcca tcggtaaaac
    26641 tctatctatc cagcaattag gagaaacgtt tgccactata cctgtatgga aaagcggact
    26701 aatgctattg attggtctta tctctgtgtt acctatggtt ggttatgata ttattttgaa
    26761 tcggatgctt gaacaaaagc agaatcctcg ttacctgttt gagacaagct ggctgatcaa
    26821 tacgatcaac aatattgccg gtttcggcgg gtttgttagt gtggggcttc gctctgaact
    26881 atatggacaa aagaaagaga gcagaaacgt gatccaagca ttatcgaaag ttttcttgtt
    26941 tttgatggct ggactttcta tttacagtct gctttctttt ttacttgtag tcttcacgcc
    27001 agttgctcct tttttgaagc aatattggat atggttgatc ggcggcggtc tatattttcc
    27061 tgtcgtcttt ttatttacta ctttcaaaaa gaaaggattc cttggtggta cctcacttaa
    27121 aactcaagga cagctattgc tagtttcatt gttggaatgg accggtgtcc tgtttagttt
    27181 cctttctgtt ggttatttaa tggaaatccg gattgatctg tggcaaacaa ttccattatt
    27241 cattgctgct tcagtgatag gaatcgtgtc tatgattcca ggagaaattg ggagtttcga
    27301 tgtaatgatg attatcgggt tgtccgctat tggtgtgcca agagaaatag tcgttgtgtg
    27361 gatcttgctc tatcgcctgt tctactacat cattccgttt ttgattggaa tcgtcttttt
    27421 cttcaaaaat atcggttcca cttttgatca gcgttattct ggtatcccta aacaattagc
    27481 aacagaaatt gcgcataaaa tcgtggttgt cttattatac ttttctggaa tcatgctagt
    27541 actttctgcc actatcccgc aagcttttac agagtttcgc tggcttcata gtttgaaccc
    27601 attgaaattt cattttatta tccaatttcc tagtatacta ttgggatttc ttttaatcat
    27661 tatgggcaga gggatcgcag cacgtgtgaa acgagcatat ttgcctacaa tcttcttgat
    27721 tgttctagct ttactttatg ttttattaag tgattttagt ttcacaccgg tggttttctt
    27781 gtctattctg cttctgatca ttcttgcttc taaaaacgaa ttatttcggg aacaattgat
    27841 ctattcatgg gagtggcgta cgatcgatgg aatcattatt ggtgcactgt ccttgcttta
    27901 catcgttatt ggggtataca atctgccaga tttccctcat cggcgtcacc attttatttc
    27961 ctttttctta tttccttctg aaaagatttg gttttcggga ttgttggcca tcatcgctgt
    28021 tagtttcatg atcgttctat ttgttcattt ccttcaagga gaaaagaaac agattggcga
    28081 agcctttaat gaagaaaagg ccctgaagat ccttacaact tatggaggaa actccgatag
    28141 tcaattgatc ttcttaaaag ataagcggat gtttgcctat gaaaaagacg gtgaaccaac
    28201 tgttcttctc caattcgctt gcttcaataa taaatgtatt gtaatgggtg atccttctgg
    28261 taagaaagaa gattttccag aagcaatcga agcttttatc gaagaaacag atcgactatg
    28321 ctatttacca gttttctacg aaacaagcga agagattgtt atgattttgc atgaattcgg
    28381 ctacgatttc attaagatgg gggaagaagc ttatgtagac ctgaatagtt ttacaacttc
    28441 agggaaaaaa atgaagggca ctcgtgctgt cttgaatcgg atcgaacgag aaggatttac
    28501 atttgatgtc ttacaaccac cattttctac tgagcaaatg agtactttca aaaatatctc
    28561 tgataattgg ttgggttcac gaaaggaaaa agggttttct ttaggctttt tctctgaaga
    28621 ttatctacaa agagcgccta ttgcagttgt caaaaatacc caagaagaaa ttgtcgcttt
    28681 tgcaacgatc atgcctactt acacgaacaa taaagtagga acgattgatc tgatgcgata
    28741 tgattctaaa aaagcacctt ctggcagtat ggatttcttg ttcttgcatt tgtttgccta
    28801 tatgaaagaa gaacatatcc aatggttcaa tttagggatg gcccctcttt caaatgtcgg
    28861 aacttccaga aagagttttt tacaggaaag gattgcatac ttggtttacg agtttggttc
    28921 tcatttttat tctttccatg gattaagaga atataaaagc aagtacgcta caaactgggt
    28981 ctcacgttat actctatatt ctagagatag ttggattgca tatgttatga ttgctttgct
    29041 gattattgat aatgcgccag ttgagcagac acaaggtaga ataagtggtt tgaaaaaatg
    29101 gatacgaaga aagtattaaa aactgcatac cgatagatga acactctcat gatgaataaa
    29161 agagactgaa gtaaaacgaa agttcatgct tctacaatct cttcttatac aattgaagaa
    29221 caaagagcgg tccaaacaga aatcctgttt ggaccgctct tttattttaa acatttttca
    29281 ttaccaaaat attcgagtcg cgtttcttat gcttcgatct ctgtaacgat acctgaacct
    29341 actgtccgtc cgccttcacg aattgagaat gtcgttccgt tttcaacagc gattggatgg
    29401 atcaagtcaa catcaatcgt cacgttgtca ccaggcatga ccatttctgt atcttctggc
    29461 aatgtgattg ttcctgtaac atcagttgtg cggaagtaga actgtggacg atagttgttg
    29521 aagaatggag tatgacgtcc accttcttct tttgtcaaca cgtacacttc tgctttgaat
    29581 tttgtatgtg gagtaatgga acctggttta gcaagtactt gtccacgttc gatatcgtca
    29641 cgttggatcc cccgtaacaa cacgcctacg ttatccccag cttccccgta atctaacgtt
    29701 ttacggaaca tttctacacc tgttacaact gctttttgtg tttcaggttt gatccctacg
    29761 atctctacct catcaccgac acgaacagca ccacgatcaa tacgacctga ggcaacagtt
    29821 cctcgacctg taattgagaa gacgtcttcc actggcaata gtaatggttt atctgtatca
    29881 cgttctggtg ttgggatata ttcatctact gtatccatca attccataat agcagcttca
    29941 gcatctggat cgccttgcaa tgcttttaat gcggaacctt tgatcacagg agtgtcatcg
    30001 cctgggaaac catattcgct caataactcg cgaacttcca tttctaccaa atcgatcaat
    30061 tcttcatcat cgactaaatc aactttgttc aagaaaacaa tcaggtattt tacaccaact
    30121 tggcgtgaca ataaaatgtg ttcacgtgtt tgaggcatcg gaccgtcagt tgcagaaaca
    30181 actaagattg caccatccat ttgtgctgcc cctgtaatca tgtttttcac atagtccgcg
    30241 tgtcctggtg catcaatatg cgcgtagtgg cgtttttctg tttcatattc tacgtgtgcg
    30301 gtattgatcg taatcccacg ttcacgttct tctggagctg catcaatact agcatagtct
    30361 tgaggatttg ctaggccttt tttgcctaat actgtagtga tcgcagcagt taaagtagtt
    30421 ttcccatggt caacgtgtcc aatcgtacca atgttaacgt gtggtttact tctgtcataa
    30481 tgttcttttg ccataattta caaacctcct aaataatata gatcactgaa aaatttcagc
    30541 tctcaccttt ttattatagt ctttttttga ctaaatcaaa aagaatacgt ccgacttact
    30601 ttttgtaaga ttcgaactaa gcttattata cgtcaatatt ggtcaaagtc aactaagagc
    30661 actcgtctgt cttatacgcg aaaaaagtac cttttataaa taaaaaaatg tgttttttga
    30721 gaaaaacttc aagatagtcc ttaactttct tttgaagcaa taagaacaaa atgaggaaaa
    30781 gtttaaaatt cagctgttgc caacaacttt caatagacga aatcttctat ttcgttaata
    30841 ataaggaaag aggtgaagta catgaaaaaa ttaattggcg tacttgtcgt tttaggcata
    30901 tttctcggcg gaagttttgc agcttatcat tatttttacg gcggcgaagc ttattataca
    30961 aaaatcacga cgaatggcga aatgtcagct acccaagcag ataacggaga gaaattcacg
    31021 acttatacct acaagcaagc ggcttataat aaagatggtg aaaaaaaaca ggtgacgctt
    31081 cgcgaagaaa gagaacatcc attaaagatg aatgcgtatc ttaaactcaa agtcaatcct
    31141 cgtaaaggtg tattaagctg ggaagaaata aaggcttctg aagtgcctaa aaatgcggca
    31201 gaaaaaatcg ataattagta aatggtctta aaattttatt agataaaaag aggctgggac
    31261 agaagcgttt aactccaaga aataagaagg agttgcttct gcttccgccg tttatacgtg
    31321 tttagagcgt gagacaaaag tgttctttac ttttgtctca cgctcttttt ttgttatatt
    31381 cgtcttttcc gatccttctc ttgacacaat tcatttaact gatcactggt acttgaacac
    31441 ctaatattct agtcattttt ataaacgatt caatacttca tccacatttt tgatcatcac
    31501 ggatatcgta ccatccggat tattcacaaa ttcaatcaaa tctgggttgc gatatacatc
    31561 tactggtacg atcagttcga tcccgtttga tagcttgaat ttttgttttc caaatttttt
    31621 ctcagaaatt tcttgtactt cttttacagg aggtgcttct gggacaaatc cttgctcttg
    31681 tacttcttct ttaaaagcca tttttgccgt gatgttttcc ttgaagactt tttccgcaat
    31741 catctcatga tccaaccgac ctttttcttc tatcgtatca tagacagctt ctttcaaatc
    31801 agccatgata tcaaattctt ctgtctcaaa tttttttcca agatgcttgg ctactttttg
    31861 gatcactttt acattttctt ctaaagaagg tgcaggaaca ctttcgatca ctcttccaga
    31921 aaaataaagc tcttttttgc cagaaaactc atattttttt tcaattaatt cgtaggtcca
    31981 atcagtcaaa ttcacagcga atgcttcatc tgctttttgt gttttcgccc ctagaattgc
    32041 tcgattcaaa atcaagcggt tgtcgatccc tgcttcgtct gcttcaacaa agtgagtgta
    32101 agcctcacga taattgactt tcaataaaga taagtacatc actgtatcta attcatacaa
    32161 gacaacaaat acatctgcac taggtgcttc ttcactttct tggtagatat cgaaccaccg
    32221 ctgaacgatt gcttcacttg cttccacaaa attttcttga gcgattttaa gtgattcaac
    32281 gatcatgctt tcttctgcta gttgtccagt ttttgtttga gcagaagaga ttttttggat
    32341 cttcttttga agaaaatcac gaatatattc ttttgtcaga tccagctctt tttgtgaaaa
    32401 gactggagaa ccagtttctc tgtcaataat atgcagaatt gcttttttta aatagatatc
    32461 cattttctca ttcccttctg tcttagttta cggaaaaaaa atcaaaaata ccagaatcaa
    32521 aatcattttc ttttaaacaa aaatttcaag agtagaaaca aaatggattc tttttttcga
    32581 aaaatgcgct acacttatag ttgaacgaat aaaaaaggag ctgaaagtat gaattcatta
    32641 acaagcactt atcgtctatc aaatggttac gaaatcccgg tagtaggatt cggcacatgg
    32701 caaacacctg atggggatgt cgctgtttcc tctgttaaag aagcgctggc tgcaggttat
    32761 cgccatatcg atactgccca aggatacaag aatgaagaaa gcgtcggcca agcgattaag
    32821 gaaagtggaa ttcctagaga agagatattc ttgacaacaa aattatggaa tgcaaaccac
    32881 agctatgaac ttgtgatgtc ttcttttgaa gaatcattga agaagctcca aacagattat
    32941 ttagatctgt ttttgatcca ctggccaaac cctgttgctt ttcgtgataa ttgggaacaa
    33001 gcaaatgcag atacatggcg agcatttgaa gaactgtatg aagcaggtaa aatcaaagca
    33061 atcggtgtga gcaatttctt accccaccat ctagatacac tagcaaaaac agcaaaaatt
    33121 atgccgatgg tcaatcaagt tttccttgca ccaggcgaat tacagacaac tgtagttgag
    33181 tatgcaaaaa aacatgatat gatcctcgaa gcatatagtc cattaggcac tggtaaaatc
    33241 ttcgatgtgc ctgaaatgaa acaaatcgct gaagcacatg acaagacgat tgcacaagtt
    33301 gctttgcgtt ggtcgctgca acacgaattc cttcctcttc ctaagtcagt gacaccaagc
    33361 agaatcaaag aaaatactga attatttgat tttgaattaa cagaagaaga aatgaaacaa
    33421 atcgatcagt tagatggtgt agtcggcaaa gcaaaagacc cagatactac ccaattttaa
    33481 gtcgaaaggt gattttatgg atccaatcga acgcttgaat tctctctcgg aagaagttac
    33541 ccagactttt cattccgatt ttgtcttttt gatcgatgca gagaaaatcc aacattttcc
    33601 tgcaagaaac tggacacatg atcaaatcat cgaagaactg aaaaaacggt tcgatcattc
    33661 gctaatggtc aaaccctggc acgaacatga agtcatttat tcacctgaac tacctgtttt
    33721 tgccttgata ccgaaaaaat agaaagaat
//
LOCUS       DACETN010000022        33209 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DACETN010000022 DACETN010000000
VERSION     DACETN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.023"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..159
                     /locus_tag="IXF98_000938"
     CDS             <1..159
                     /locus_tag="IXF98_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1555270.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
     gene            complement(540..878)
                     /locus_tag="IXF98_000939"
     CDS             complement(540..878)
                     /locus_tag="IXF98_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1555271.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(1013..1414)
                     /locus_tag="IXF98_000940"
     CDS             complement(1013..1414)
                     /locus_tag="IXF98_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555272.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            1526..2146
                     /locus_tag="IXF98_000941"
     CDS             1526..2146
                     /locus_tag="IXF98_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1555273.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            complement(2237..4906)
                     /locus_tag="IXF98_000942"
     CDS             complement(2237..4906)
                     /locus_tag="IXF98_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1555274.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(5144..5332)
                     /locus_tag="IXF98_000943"
     CDS             complement(5144..5332)
                     /locus_tag="IXF98_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1555275.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            5602..5964
                     /locus_tag="IXF98_000944"
     CDS             5602..5964
                     /locus_tag="IXF98_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1555276.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6016..7698
                     /locus_tag="IXF98_000945"
     CDS             6016..7698
                     /locus_tag="IXF98_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1555277.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            7815..9851
                     /gene="recG"
                     /locus_tag="IXF98_000946"
     CDS             7815..9851
                     /gene="recG"
                     /locus_tag="IXF98_000946"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1555278.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            9900..10901
                     /gene="plsX"
                     /locus_tag="IXF98_000947"
     CDS             9900..10901
                     /gene="plsX"
                     /locus_tag="IXF98_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1555279.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            11023..11265
                     /gene="acpP"
                     /locus_tag="IXF98_000948"
     CDS             11023..11265
                     /gene="acpP"
                     /locus_tag="IXF98_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1555280.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            11533..12537
                     /locus_tag="IXF98_000949"
     CDS             11533..12537
                     /locus_tag="IXF98_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555281.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            12538..13482
                     /locus_tag="IXF98_000950"
     CDS             12538..13482
                     /locus_tag="IXF98_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555282.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            13482..14444
                     /locus_tag="IXF98_000951"
     CDS             13482..14444
                     /locus_tag="IXF98_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555283.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            14471..15385
                     /locus_tag="IXF98_000952"
     CDS             14471..15385
                     /locus_tag="IXF98_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555284.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            15411..17192
                     /locus_tag="IXF98_000953"
     CDS             15411..17192
                     /locus_tag="IXF98_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1555285.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            17540..18226
                     /locus_tag="IXF98_000954"
     CDS             17540..18226
                     /locus_tag="IXF98_000954"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1555286.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            18246..21827
                     /gene="smc"
                     /locus_tag="IXF98_000955"
     CDS             18246..21827
                     /gene="smc"
                     /locus_tag="IXF98_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1555287.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            21836..22645
                     /locus_tag="IXF98_000956"
     CDS             21836..22645
                     /locus_tag="IXF98_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1555288.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            22657..23655
                     /gene="ftsY"
                     /locus_tag="IXF98_000957"
     CDS             22657..23655
                     /gene="ftsY"
                     /locus_tag="IXF98_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1555289.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            23842..26109
                     /gene="gshAB"
                     /locus_tag="IXF98_000958"
     CDS             23842..26109
                     /gene="gshAB"
                     /locus_tag="IXF98_000958"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1555290.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(26197..26649)
                     /locus_tag="IXF98_000959"
     CDS             complement(26197..26649)
                     /locus_tag="IXF98_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1555291.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            26799..27749
                     /locus_tag="IXF98_000960"
     CDS             26799..27749
                     /locus_tag="IXF98_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1555292.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            27742..28701
                     /locus_tag="IXF98_000961"
     CDS             27742..28701
                     /locus_tag="IXF98_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1555293.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            28698..29453
                     /locus_tag="IXF98_000962"
     CDS             28698..29453
                     /locus_tag="IXF98_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1555294.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            29476..30429
                     /locus_tag="IXF98_000963"
     CDS             29476..30429
                     /locus_tag="IXF98_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1555295.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(31073..31369)
                     /locus_tag="IXF98_000964"
     CDS             complement(31073..31369)
                     /locus_tag="IXF98_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1555296.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            31725..32081
                     /locus_tag="IXF98_000965"
     CDS             31725..32081
                     /locus_tag="IXF98_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1555297.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            32091..32990
                     /locus_tag="IXF98_000966"
     CDS             32091..32990
                     /locus_tag="IXF98_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1555298.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        11011 a         5876 c         6956 g         9366 t
ORIGIN      
        1 attaaaaatc atgaaaaatt cttgattaga aattataaga ttagtggagg gggctgttat
       61 cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca gctttttgga acagcttaat
      121 gaatatgcga attataaatt atctattgtt atagattaat aataaaacta gcagatggta
      181 aaaatactaa tatgcgagac acacattcaa ccataactat ataacttatc aatttaaaac
      241 taaactgatg gaaacaagta aggcaaaaac atttatattt tgccctactt atttttttaa
      301 ttaaattcat tgatgattca tatttagaaa attctatgaa ggccaaatga gtatcctgtc
      361 acttttttag ataaatagca aaaattttca gcgttaaatg acaaataatt agaaatattt
      421 gtgccattta aatataaaaa tgacaccaag tatatcatga acgcacaaaa agaccaacag
      481 ggtataccct gttggtctaa ataaatgtca aattccgttc tcaactgtgc atattcattc
      541 tatgttccat gtaccccatt atcctcctga atactcattc caatgttagt taatttttct
      601 aattcttcta cgcttagata agcgccatat tttacaattt cctcatcaat gacaataatc
      661 ggtaaacttt tcaaatcttt tgtctgcaac aagttaagaa ttttagggtt gctaataaaa
      721 ggcaagatag agtcacttaa aaaatataaa tttgtttcta tctgtttatt cttaatccga
      781 ttataaaact cttttagcat cgtatcctca tgtaatgatt gaaaaacgaa gagtccttca
      841 tcatcacttc tatttaagac atagtaagaa attatcattt tcctattgtc aatcctcccg
      901 cagtgcaaga gactgaccct acaccaaatg tattataaac ttgcatagct aattttacac
      961 tgtaatattg tcttttccca gtaaatgtaa aatacattcc tccaccataa ccttaagaat
     1021 tagctagatt tactgtttga gtatatgatt ttgcactacc taaactcaag cgtgtagaaa
     1081 tacgatacat cgccgaattg ggacttgtta taatatattc tgtccgcccc tgcgtattag
     1141 taattttttt agtagatgcg gttaaccgca atacatttgc agaaaaacgt gttttcggat
     1201 cttgaggctc tatcttttga gtgtaaaaag tatctttatc actaaatcct ttggatttga
     1261 agtaactttg ttcttctggt gttaattgac taaaagtttt tgccatttca cttgtttgtt
     1321 caaccatcgt gctagcttta actatatttc cagaagtccc aataccaata aagccaaata
     1381 acagaataaa aagtgcgcta aataattttt tcatagtaat cacttcctaa ttcattaaat
     1441 ttttaggaaa acgctttcta aatctcgatt atacatctcc ttttaaaaat gtacaccgtt
     1501 attttttgaa tattcagact ttttcttgtt cttgataaat gcaacagatg aaatattaag
     1561 tgtaaataaa gttttttata ctgtaccagc tgaaaagatg tctcgtgtca gttcggatat
     1621 taacacaata actagtagaa taggtctgac taaagcaatg catatggtac taaaatacaa
     1681 tcaagaagat cctcctgtgc ctaaaaccta tgaagcaatt gacttaatta ttgtcgatga
     1741 aattgatcgt ttaaagcttc agcatttaga acagctaaga gatatttacg atagaaatga
     1801 tctggcaatg gttcttatcg gtatgcctgg tattgaaaaa agattgtctc gttatccgca
     1861 gctttactct cgcataggat ttgcacatga atttgataac cttagtaagg acgaaacaca
     1921 tcatattttg gaatacaaat gggcagattt aggaattgat atcaagctag aagatttttc
     1981 tgattatgag gctatcacaa ctatcattaa gatcacaaaa ggaaatttta ggcttattca
     2041 tcgtttattt gcgcagattg atcggatttt aagaatcaat caaatggata agatcactgt
     2101 agaagttgtc gaagcagcaa gagatagttt agtcataggt atataaaaaa ccaccaaaaa
     2161 tcaggtggtt tttgtcattt aaagattgaa atttttgcca tttaaataga agagtgacac
     2221 agattccatc gtttctttat tttcttcgtc ttcttctgtg tgtatttcgc ggtttttctt
     2281 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
     2341 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
     2401 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
     2461 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
     2521 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
     2581 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
     2641 caacatctag accaacgact ttaggctcca tgaaacttac ttctttcgtt ccataaaaac
     2701 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
     2761 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
     2821 ctaattgtgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
     2881 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
     2941 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
     3001 aatactgttc atttaaacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
     3061 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
     3121 cattgacatt ctgatacagt gtatagtcgc cactttgacc agcttggcgg aagccatatt
     3181 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatta
     3241 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
     3301 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
     3361 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
     3421 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
     3481 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
     3541 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
     3601 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
     3661 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
     3721 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacga tcgtcttgtt
     3781 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
     3841 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
     3901 gatagaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
     3961 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
     4021 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
     4081 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
     4141 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
     4201 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
     4261 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
     4321 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
     4381 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
     4441 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
     4501 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
     4561 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
     4621 gtgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
     4681 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
     4741 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
     4801 tcccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
     4861 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
     4921 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
     4981 tctaattttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
     5041 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
     5101 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
     5161 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
     5221 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
     5281 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
     5341 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
     5401 tatagaatgg aaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
     5461 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
     5521 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
     5581 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
     5641 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
     5701 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
     5761 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
     5821 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
     5881 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
     5941 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
     6001 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
     6061 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
     6121 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
     6181 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
     6241 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
     6301 tctaagcaaa ttccagatgt tgtctcatta aatgcttccg atcttgctgc agccttcact
     6361 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
     6421 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
     6481 gaagtaatga cagctgttgt aaaaggggca aagcgtgctt tagctaaaac tccagacttg
     6541 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
     6601 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
     6661 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
     6721 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
     6781 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
     6841 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
     6901 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
     6961 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
     7021 gcgaaaaaag aaacaccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
     7081 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg gcaaacaatg
     7141 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
     7201 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
     7261 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
     7321 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
     7381 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
     7441 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
     7501 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
     7561 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
     7621 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
     7681 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
     7741 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaggga
     7801 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
     7861 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
     7921 attatccttt ccgctacgaa gatattcagg aaagaaatct aaacgagatc caagatcaag
     7981 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
     8041 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
     8101 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
     8161 aatgggatgc aaagcgtaaa gcattgaacg ggatgaagat tttaggttct caaacaatgg
     8221 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
     8281 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
     8341 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
     8401 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
     8461 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
     8521 tcctttatga tgtccaaaaa ttgaaagaat ttactcaaaa gctacctttt gaactgacaa
     8581 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
     8641 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
     8701 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
     8761 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattgc
     8821 ttacggggtc aacgaaagca aaagaacgtc gtgagctcct tgaaaaacta gagcaagggg
     8881 aaattaacat cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
     8941 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
     9001 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
     9061 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
     9121 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
     9181 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
     9241 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
     9301 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
     9361 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
     9421 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
     9481 tggcacagct gcatcaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
     9541 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
     9601 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
     9661 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
     9721 atgttttgga ggtagccaga gaagaggctg cagccctttg gcagataaag gattggcaga
     9781 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
     9841 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
     9901 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
     9961 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    10021 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    10081 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    10141 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    10201 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    10261 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    10321 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    10381 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    10441 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    10501 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    10561 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    10621 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    10681 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    10741 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    10801 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    10861 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagca
    10921 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    10981 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    11041 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    11101 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    11161 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    11221 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    11281 aagaaggata aaaagctgca tacagctttt tatccttctt tttttaatct ttttgacacg
    11341 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    11401 attttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    11461 acgctttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    11521 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    11581 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    11641 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    11701 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    11761 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    11821 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    11881 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    11941 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    12001 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    12061 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    12121 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    12181 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    12241 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    12301 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    12361 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    12421 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    12481 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    12541 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    12601 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    12661 aagacatatg gtttagtggg agaatctggc tctggaaaat ctactacggg aaaagccgtt
    12721 gtgggattag aaaaggtgac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    12781 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    12841 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    12901 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    12961 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    13021 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    13081 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    13141 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    13201 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    13261 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    13321 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    13381 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    13441 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    13501 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    13561 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    13621 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    13681 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    13741 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    13801 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    13861 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    13921 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    13981 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    14041 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    14101 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    14161 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    14221 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    14281 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    14341 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    14401 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    14461 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    14521 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    14581 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    14641 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    14701 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    14761 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    14821 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    14881 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    14941 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    15001 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    15061 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    15121 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    15181 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    15241 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    15301 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    15361 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    15421 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    15481 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    15541 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    15601 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    15661 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    15721 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    15781 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    15841 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    15901 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    15961 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    16021 aatcgggtgg tggtatttgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    16081 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    16141 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    16201 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    16261 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    16321 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    16381 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    16441 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    16501 taggtgagcg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    16561 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    16621 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    16681 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    16741 tggcagatta ctatatccaa cagtggaaag aaatcggttt ggatgtccaa tacacaacag
    16801 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaatgatgat cctgaaattg
    16861 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    16921 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    16981 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    17041 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    17101 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    17161 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    17221 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    17281 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    17341 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    17401 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    17461 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    17521 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    17581 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    17641 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    17701 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    17761 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    17821 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    17881 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    17941 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    18001 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    18061 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    18121 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggaa aatcgaaaaa
    18181 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    18241 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    18301 tcattgactt tgaaaatagc gtcacagcag tggttggacc aaatggcagt ggaaaaagca
    18361 atattacaga agctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    18421 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    18481 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    18541 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    18601 gcagattaaa agatatccaa gaattgttct tagactctgg attaggaaaa gaatcgttct
    18661 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    18721 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    18781 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    18841 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    18901 aagaaacact gactcaaacc gatgtctctt tgatggttgc cgaaatcaag acagcaaaaa
    18961 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    19021 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    19081 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    19141 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    19201 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    19261 aagaaagctt gttgaaagaa gtagcagaaa aagaaacaga aatccaaaaa gctgaagcaa
    19321 gtctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattactgg
    19381 cagaactaag agatcaatat gttgatttga tgcaagagca agcagctgta ggaaacgaac
    19441 tgaagtatct agaacgtcaa tatgtacaag aaacagcaaa aagtaagcag acgttagcca
    19501 agcaatcgga agtagaggca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    19561 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    19621 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    19681 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    19741 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    19801 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    19861 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    19921 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    19981 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    20041 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    20101 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    20161 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    20221 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    20281 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    20341 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    20401 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctgcaaaaca
    20461 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    20521 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    20581 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    20641 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    20701 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    20761 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    20821 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    20881 tatctgctca gcgagaaaca ttgaaagccg agttggcaaa agccaaagag cagcgagata
    20941 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    21001 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    21061 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    21121 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    21181 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    21241 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    21301 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    21361 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    21421 aattagtact gacagaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    21481 ctcctggcaa aaaactgcaa agtttaagtt tgctttctgg aggagaacga gcacttacag
    21541 ccatcgcgtt gctcttttct attatccgtg tctgtcctgt tccgttttgt atcttagatg
    21601 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    21661 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    21721 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    21781 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    21841 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    21901 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    21961 aggacgcccc ttagctgcga tggtgcatta tctgcaagaa ttaggactgg tagatgaagg
    22021 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    22081 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    22141 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    22201 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    22261 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    22321 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    22381 gagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    22441 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcaatcggtg atgaagaaaa
    22501 tgatttaccg atgatccaat acgctggact aggcgtagcg atggagaatg ctgtgccttt
    22561 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    22621 gatccaaaaa tttgtattag agtagggggg agtcaaatgg gattttttga taaaatcaag
    22681 cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag
    22741 ggtttagaaa agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt
    22801 cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta
    22861 ggttttgaaa cagcgatacg aatcaccgaa gcattacgtc aagaagtgaa actcagaaat
    22921 gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa
    22981 gcggaaggaa tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt
    23041 ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag
    23101 ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca
    23161 atcgaccagt tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca
    23221 ggcggcgatc cagcagctgt cgtattcgat gctttggaaa gagcaaagat agagcaggca
    23281 gatgttttac tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa
    23341 ttagaaaaaa taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt
    23401 cttgtactag atgcaacaac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa
    23461 acaacagatg tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc
    23521 gtacttgcta tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga
    23581 atcgatgact tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta
    23641 ctaaaagaag aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt
    23701 gtttgaatga ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt
    23761 gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc
    23821 atatgaattg gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg
    23881 cgccctttta tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt
    23941 gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc
    24001 tatatccaaa cagattttag cgaaacacaa acagagatga tcactcctgt taccgattct
    24061 attcccgaat tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg
    24121 aaagaagaga tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagcg
    24181 atcattattg caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa
    24241 gaatacggga agcgaaaaca gatggtaagc ggtatccatt tcaattttga atttggcgat
    24301 gagttgctaa gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa
    24361 acagaacttt atttgaaaac ggcgagaaac tttatgcgct atcgctggat gatcacttat
    24421 ttattcggtg cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa
    24481 gaacctgttc gcagtattcg taacagtgcg ctgggctata cgaatcatcc aaatgtgaaa
    24541 gtttcctatg cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg
    24601 aaactttcgg aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc
    24661 gcagatttag caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct
    24721 tatgcaagat tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac
    24781 atgttatgga cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga
    24841 aataacaaag tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga
    24901 gaaattcttg aagggatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat
    24961 ttagtagagg aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc
    25021 tatcaagaag cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat
    25081 tataaagaaa gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct
    25141 actcagatat ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag
    25201 tcggatcaat ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac
    25261 atgacaagta aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa
    25321 aaagtcttaa aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa
    25381 gaagcggtaa aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca
    25441 accaattacg ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa
    25501 gcaggtttag ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg
    25561 ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc
    25621 cctgctaatg ttattggtga cggtatccga acagtgaaag aacttgttga agaaaaaaat
    25681 agtgaccctt tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa
    25741 ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat
    25801 caaattgttt atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg
    25861 actgatgaat tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga
    25921 gctaagatta gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat
    25981 aaaaaagcat atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat
    26041 ccctttgcag gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa
    26101 gtattttaaa agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag
    26161 cttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    26221 ttttttcttt tttggttttt ctttttcaac tggcgaagag ttttcttctg ggtatttttt
    26281 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    26341 ctcaggattt gcagtgacgt aatcgttgac tacagtaaat cggctattgt ttttcgttgc
    26401 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    26461 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    26521 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    26581 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    26641 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    26701 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    26761 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    26821 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    26881 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    26941 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    27001 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    27061 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    27121 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    27181 cccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    27241 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    27301 aaggaaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    27361 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    27421 ttgcagtaaa tttgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    27481 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    27541 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    27601 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    27661 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    27721 ttctgataag ggggcgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    27781 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    27841 tttgcatggg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    27901 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    27961 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    28021 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    28081 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    28141 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    28201 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    28261 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    28321 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    28381 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    28441 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    28501 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    28561 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    28621 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    28681 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    28741 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc
    28801 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    28861 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    28921 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    28981 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    29041 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    29101 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    29161 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    29221 tgcagatctt aaaaaaatta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    29281 atatcaactt tgctgcaagt tatgcagacg aaattattgc aatgaaagat gggcagttat
    29341 tcacgcaagg aacgacccaa gaaatgataa aaaaagatgt actggacgaa ttgtacgaaa
    29401 tggacatacg aatttgtgag attgaaggtc gcagattctg tctttatttc taaaactaac
    29461 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    29521 ttagcagcat gtggaaatag tgacaacacc agtcaggcag atacaaagaa aagcagtacg
    29581 caagctgaaa cggcattaac gataaaagac agtaatggcg atcaaatcga ggtgccaaag
    29641 aatcctgaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    29701 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    29761 aaaaaagtgg aatcagcagg tgggatcaaa gaaccagact tagaaaaaat caatcagctt
    29821 cagccagacc tgatcatcat ttctggccgg caaagagatt ttcaaaaaga tctaagtcaa
    29881 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    29941 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    30001 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    30061 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    30121 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    30181 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    30241 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    30301 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    30361 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    30421 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    30481 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    30541 gtattgatga ttttacggat ggtttttatt tatttgggaa atcttttttc aacgtgaatt
    30601 cattctctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    30661 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    30721 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    30781 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    30841 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    30901 catctgctgc ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg
    30961 tccagcgttc ttattactta aagcaaatcg tagttgtcac aatctcttct aaacgatgtt
    31021 ccatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    31081 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    31141 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    31201 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    31261 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    31321 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    31381 caaagaagtc tttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa
    31441 caaaaagtaa ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt
    31501 gttatttaag aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg
    31561 ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa
    31621 taaacgaatg acttactttc caataaattg agaacagtca gaaccagaca aattagttgc
    31681 atctgatgaa taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga
    31741 caaaagaagc acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag
    31801 atatcgaaaa cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggacacgg
    31861 catttcgatt gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag
    31921 tggctgatac accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg
    31981 atccatccac acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg
    32041 gcttgagaaa aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat
    32101 tggcgatcat cagcgaccta catgtggaca tcaagaaatt cggcgaatca gagctgatgc
    32161 agctttggca agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg
    32221 ctaataaagt agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta
    32281 cgacatttca tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc
    32341 attttccaga cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc
    32401 ttttagggat gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa
    32461 tcgtacgaat gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc
    32521 tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa
    32581 aaaaacttga ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc
    32641 aatctgctcc ttatgagcgc tggaacaaat taaatgcatt tttaggatca gaatcattcg
    32701 gagaattact tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc
    32761 gctttgaaga taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt
    32821 atgaatggca gctgacccga gattttgtct tgaaagaaca gctgttagat agctatgaac
    32881 ccatgaaatt acgtgtcctc ttgcgtgccc atcatccggc ctttcaagcc tatcaaacac
    32941 aacatttgca agaggaattt gaacaggcga tgacaatcgt tccgtattga gacagcatta
    33001 gtaatgtgta aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat
    33061 ttatcgtgga tatgctgctt tttttgtgga aaataatcag cgaatgttta tttgatcgga
    33121 tgatttgtaa ctcgcagagt actagatgca agcgtttatt ctttttttga acgataatca
    33181 caaaaaaaaa tataatatac attttttat
//
LOCUS       DACETN010000023        32292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DACETN010000023 DACETN010000000
VERSION     DACETN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.024"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..226
                     /locus_tag="IXF98_000967"
     CDS             <1..226
                     /locus_tag="IXF98_000967"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1555299.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IXF98_000968"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IXF98_000968"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IXF98_000969"
     CDS             533..1336
                     /locus_tag="IXF98_000969"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1555300.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="IXF98_000970"
     CDS             complement(1585..2649)
                     /locus_tag="IXF98_000970"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1555301.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="IXF98_000971"
     CDS             complement(2646..3731)
                     /locus_tag="IXF98_000971"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1555302.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IXF98_000972"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IXF98_000972"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1555303.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IXF98_000973"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IXF98_000973"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1555304.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5980..6633
                     /locus_tag="IXF98_000974"
     CDS             5980..6633
                     /locus_tag="IXF98_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1555305.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6718..7623
                     /locus_tag="IXF98_000975"
     CDS             6718..7623
                     /locus_tag="IXF98_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1555306.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="IXF98_000976"
     CDS             complement(7625..8257)
                     /locus_tag="IXF98_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1555307.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="IXF98_000977"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="IXF98_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1555308.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="IXF98_000978"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="IXF98_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1555309.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="IXF98_000979"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="IXF98_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1555310.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10137..11375
                     /locus_tag="IXF98_000980"
     CDS             10137..11375
                     /locus_tag="IXF98_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1555311.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11423..13552
                     /locus_tag="IXF98_000981"
     CDS             11423..13552
                     /locus_tag="IXF98_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1555312.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="IXF98_000982"
     CDS             13527..14216
                     /locus_tag="IXF98_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555313.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="IXF98_000983"
     CDS             complement(14624..15310)
                     /locus_tag="IXF98_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1555314.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="IXF98_000984"
     CDS             complement(15446..15640)
                     /locus_tag="IXF98_000984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555315.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="IXF98_000985"
     CDS             complement(15690..16130)
                     /locus_tag="IXF98_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1555316.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="IXF98_000986"
     CDS             complement(16134..16979)
                     /locus_tag="IXF98_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1555317.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="IXF98_000987"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="IXF98_000987"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1555318.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="IXF98_000988"
     CDS             complement(18622..19824)
                     /locus_tag="IXF98_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555319.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="IXF98_000989"
     CDS             complement(19811..21598)
                     /locus_tag="IXF98_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555320.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="IXF98_000990"
     CDS             complement(21611..22564)
                     /locus_tag="IXF98_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1555321.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="IXF98_000991"
     CDS             complement(22749..23099)
                     /locus_tag="IXF98_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1555322.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="IXF98_000992"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="IXF98_000992"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1555323.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="IXF98_000993"
     CDS             24521..24841
                     /locus_tag="IXF98_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1555324.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="IXF98_000994"
     CDS             24863..25327
                     /locus_tag="IXF98_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1555325.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /locus_tag="IXF98_000995"
     CDS             25523..26128
                     /locus_tag="IXF98_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1555326.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="IXF98_000996"
     CDS             complement(26167..27456)
                     /locus_tag="IXF98_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1555327.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="IXF98_000997"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="IXF98_000997"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1555328.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28716..29030
                     /locus_tag="IXF98_000998"
     CDS             28716..29030
                     /locus_tag="IXF98_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1555329.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29046..29432
                     /locus_tag="IXF98_000999"
     CDS             29046..29432
                     /locus_tag="IXF98_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1555330.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29461..30846
                     /locus_tag="IXF98_001000"
     CDS             29461..30846
                     /locus_tag="IXF98_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1555331.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            30849..31002
                     /locus_tag="IXF98_001001"
                     /pseudo
     CDS             30849..31002
                     /locus_tag="IXF98_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            31025..32230
                     /locus_tag="IXF98_001002"
     CDS             31025..32230
                     /locus_tag="IXF98_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1555332.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT        10233 a         6043 c         6617 g         9399 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcta gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agatgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatcta tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 ttattattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
    28441 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
    28501 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
    28561 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
    28621 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
    28681 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
    28741 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
    28801 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
    28861 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
    28921 aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc
    28981 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
    29041 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
    29101 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
    29161 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
    29221 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
    29281 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
    29341 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
    29401 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
    29461 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
    29521 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
    29581 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
    29641 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
    29701 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
    29761 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
    29821 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
    29881 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
    29941 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
    30001 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
    30061 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
    30121 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
    30181 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
    30241 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
    30301 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
    30361 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
    30421 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
    30481 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
    30541 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatcagtt taatttcaga
    30601 gtggctttag gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag tgacgtacaa
    30661 aaggatttct tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc
    30721 atatcagagt tttatactcc ccttgtacca aaaggatatg atttcttgga ggaaattaaa
    30781 aagttatcca acagcagaca gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg
    30841 gactgatctt gctggctggt gtggcaatag ccacgccagc acttaacccc tcggtatcta
    30901 acaggggggt acaaatcgac aggaaacagt caaaaaaaca ttagaaaatc ctttggttac
    30961 aagggattta caaaatttca gcgtatgtca aatgggcttt aaaagttgac atacgccttt
    31021 ttgattggag ggatttttac tgaatgaaca aacttggtta cagcatttaa aagaaaaacg
    31081 cttggcttat ggactatctc aaaaccgttt agctgttgcg actggtatta caaggcagta
    31141 tctaagcgat attgaaacag gaaaagtcaa gccatcagag gatttacagc agtccctttg
    31201 ggaagctctg gaacgcttca atcccgacgc tccccttgaa atgctgtttg attatgtaag
    31261 aattcgcttt ccgacaacag acgtacagca ggtggtcgaa aacatcttac aactgaaact
    31321 gtcctatttt cttcatgagg actatggttt ctattcttat tcagagcatt atgctttagg
    31381 cgacatattc gtcctttgct cccatgaact ggacaaagga gttctggtgg aattgaaagg
    31441 tcgtgggtgc agacaatttg aaagctatct tctggcacaa caaagaagct ggtatgagtt
    31501 ctttatggac gttttggtgg ctggcggtgt gatgaaacgc cttgaccttg ccattaacga
    31561 taagacaggg attttaaata tccctgtact cactgaaaag tgccaacagg aagaatgtat
    31621 ctccgtcttc cgcagtttta aaagctatcg cagtggcgaa ctggtacgca aagaggaaaa
    31681 ggaatgtatg ggaaacaccc tctatatcgg ttcattacaa agtgaagttt atttctgtat
    31741 ctatgaaaag gactacgagc agtacaagaa aaatgatatt cccattgaag acgcagaagt
    31801 aaaaaaccgt tttgagattc gattgaaaaa tgagcgtgcc tattatgcag tccgtgattt
    31861 actcgtctat gacaatccag agcataccgc ctttaaaatt atcaatcggt atatccgttt
    31921 tgtagataaa gacgattcca aacctcgttc tgattggaaa ctgaatgaag aatgggcttg
    31981 gtttattggg aacaatcgtg aacgattaaa actaaccaca aaaccagagc cttactcctt
    32041 ccaaaggacg ctgaactggc tatctcatca agttgccccg accttaaagg ttgcgattaa
    32101 acttgatgaa atcaaccaga cgcaggttgt aaaagacatt ctcgaccatg cgaaactgac
    32161 agaccgacac aagcagattt tgaagcaaca gtcagtaaaa gaacaggacg tgataacaac
    32221 aaaaaaataa ctcaaataca aattcattga atatagagag gagaacattt ttatgaattt
    32281 tggacaaaac ct
//
LOCUS       DACETN010000024        30947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DACETN010000024 DACETN010000000
VERSION     DACETN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.025"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            82..1395
                     /locus_tag="IXF98_001003"
     CDS             82..1395
                     /locus_tag="IXF98_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1555333.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            1392..2111
                     /locus_tag="IXF98_001004"
     CDS             1392..2111
                     /locus_tag="IXF98_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1555334.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            2239..2790
                     /gene="raiA"
                     /locus_tag="IXF98_001005"
     CDS             2239..2790
                     /gene="raiA"
                     /locus_tag="IXF98_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1555335.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            3012..5546
                     /gene="secA"
                     /locus_tag="IXF98_001006"
     CDS             3012..5546
                     /gene="secA"
                     /locus_tag="IXF98_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1555336.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            5672..6773
                     /gene="prfB"
                     /locus_tag="IXF98_001007"
     CDS             join(5672..5734,5736..6773)
                     /gene="prfB"
                     /locus_tag="IXF98_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1555337.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            6878..7564
                     /gene="ftsE"
                     /locus_tag="IXF98_001008"
     CDS             6878..7564
                     /gene="ftsE"
                     /locus_tag="IXF98_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1555338.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            7548..8441
                     /locus_tag="IXF98_001009"
     CDS             7548..8441
                     /locus_tag="IXF98_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555339.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            8593..10041
                     /gene="cls"
                     /locus_tag="IXF98_001010"
     CDS             8593..10041
                     /gene="cls"
                     /locus_tag="IXF98_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1555340.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            10378..11235
                     /locus_tag="IXF98_001011"
     CDS             10378..11235
                     /locus_tag="IXF98_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1555341.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            11350..12270
                     /gene="pstC"
                     /locus_tag="IXF98_001012"
     CDS             11350..12270
                     /gene="pstC"
                     /locus_tag="IXF98_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1555342.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            12270..13154
                     /gene="pstA"
                     /locus_tag="IXF98_001013"
     CDS             12270..13154
                     /gene="pstA"
                     /locus_tag="IXF98_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1555343.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            13169..13975
                     /gene="pstB"
                     /locus_tag="IXF98_001014"
     CDS             13169..13975
                     /gene="pstB"
                     /locus_tag="IXF98_001014"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555344.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            13991..14749
                     /locus_tag="IXF98_001015"
     CDS             13991..14749
                     /locus_tag="IXF98_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555345.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            14762..15439
                     /gene="phoU"
                     /locus_tag="IXF98_001016"
     CDS             14762..15439
                     /gene="phoU"
                     /locus_tag="IXF98_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1555346.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            15677..16708
                     /locus_tag="IXF98_001017"
     CDS             15677..16708
                     /locus_tag="IXF98_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1555347.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            16705..17472
                     /locus_tag="IXF98_001018"
     CDS             16705..17472
                     /locus_tag="IXF98_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1555348.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            17572..19140
                     /gene="liaX"
                     /locus_tag="IXF98_001019"
     CDS             17572..19140
                     /gene="liaX"
                     /locus_tag="IXF98_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1555349.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            19440..19757
                     /locus_tag="IXF98_001020"
     CDS             19440..19757
                     /locus_tag="IXF98_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1555350.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            19757..20107
                     /locus_tag="IXF98_001021"
     CDS             19757..20107
                     /locus_tag="IXF98_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1555351.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            20274..21212
                     /locus_tag="IXF98_001022"
     CDS             20274..21212
                     /locus_tag="IXF98_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1555352.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            21227..22057
                     /locus_tag="IXF98_001023"
     CDS             21227..22057
                     /locus_tag="IXF98_001023"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1555353.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            22101..23126
                     /locus_tag="IXF98_001024"
     CDS             22101..23126
                     /locus_tag="IXF98_001024"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1555354.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            23141..24079
                     /gene="galU"
                     /locus_tag="IXF98_001025"
     CDS             23141..24079
                     /gene="galU"
                     /locus_tag="IXF98_001025"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1555355.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            24204..24644
                     /locus_tag="IXF98_001026"
     CDS             24204..24644
                     /locus_tag="IXF98_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1555356.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            24647..25249
                     /locus_tag="IXF98_001027"
     CDS             24647..25249
                     /locus_tag="IXF98_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1555357.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(25312..26415)
                     /locus_tag="IXF98_001028"
     CDS             complement(25312..26415)
                     /locus_tag="IXF98_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1555358.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            26625..27626
                     /gene="ccpA"
                     /locus_tag="IXF98_001029"
     CDS             26625..27626
                     /gene="ccpA"
                     /locus_tag="IXF98_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1555359.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            27981..28376
                     /locus_tag="IXF98_001030"
     CDS             27981..28376
                     /locus_tag="IXF98_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555360.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            28376..28627
                     /locus_tag="IXF98_001031"
     CDS             28376..28627
                     /locus_tag="IXF98_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555361.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            28670..29065
                     /locus_tag="IXF98_001032"
     CDS             28670..29065
                     /locus_tag="IXF98_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555362.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            29062..30318
                     /locus_tag="IXF98_001033"
     CDS             29062..30318
                     /locus_tag="IXF98_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1555363.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            30330..30653
                     /locus_tag="IXF98_001034"
     CDS             30330..30653
                     /locus_tag="IXF98_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555364.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            30646..30912
                     /locus_tag="IXF98_001035"
     CDS             30646..30912
                     /locus_tag="IXF98_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555365.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        10412 a         5327 c         6670 g         8538 t
ORIGIN      
        1 aatagaacag tataactatc gaacaagcga tccaaaaaat agatcgcttg ttttttcgta
       61 tattttttta gggcgaataa tatgaatcat ttttggggta gaaggcttct gacgagagat
      121 attaaagcaa tagactgtga ggaggtaaat ttaccaaatt atgagaagat cgcagcagtg
      181 gaacgtttgg gcaactggat tctttgtcat cgctgtggaa tgaggacgtc cgtcattgat
      241 ggacagcttg cagattacgg ttatttttgt acacattgtc tatatttagg gcgatgcgac
      301 actcaacaag atttatattt gtttgatcag cctgaagcaa aacctcgaga agtcgtcttt
      361 ttttggacag gacagcttac tgaaaaacaa acggaaatcg caaagagaat actttgccat
      421 tcagaaaaaa gacatcatct tatttgggct gtaacaggtg cagggaaaac agagatgctt
      481 tatcctattt tagtggaaac tttaaaagct ggaggaagag tagcgatttg tacaccgcga
      541 gtcgatgttt gtaatgaatt gtttttacgc tatcgttatg tttttccaga agaaaaaatc
      601 acgttgctcc atgggaatac agcaacggct tataccttta cttcttttgt tatctgtacg
      661 attcatcagc tataccgttt ttatcggtcc tttgatttaa tagtgatcga tgagatcgat
      721 gcgtttcctt ataacgggga tgcaggatta gctcatgctg tgcaaacagc gattaaacca
      781 gacggacgat tcatctactt aagtgccaca cctgataaaa agttactaaa ggaaatcaag
      841 cagacgtttg acatccacaa acttgctttg cgctttcatc ggcgtttact tcctgaaccc
      901 gtcttggttt tcatgaataa ttggcgacaa aaatgcaaga acaagaagaa gataaaatct
      961 ttggtgcgga tcattcatca attaattaaa gaaaaccata tccttttatt ttgtccaagt
     1021 atcacgatga tgcatcgttt gaaagccaca cttcaagagg ctcttccgag atatgagatc
     1081 acagatgttt cttcaagaga caatcagcgg gcggaaaaag tccagaaaat gcgagaagca
     1141 aaatatgaca tactgttaac cacgatgatc cttgaacgtg gagtcacatt tgaaagaata
     1201 tccgttatcg ttttaggagc ggatcatccg gtttttacta aatcttcttt ggtacaaatc
     1261 gcaggaagag ccgatcgtaa agggccttat acgaacagtc aagtatactt tttctatgaa
     1321 gaaaaaactg cagctattcg aaaagcttgt caggaaatca aagaaatgaa tgaagaaggg
     1381 aagaagatgc tgtgaggtgc agttgctgca gtaaagatat cgtccaaaat ttaacattca
     1441 gcgagattat tggtgtttcg aaatacagat tacctcagac ttgttcagtt tgtcgcgcac
     1501 aatttcattt attggaaaca agaggctgtt ctggctgtca aaaaccaatc catttcaatc
     1561 tagagagtac gaaaaagaaa gtaaagtcta ttcccgttta ctgcagtgac tgtcaagaat
     1621 ggaagaagaa atatccgacc tatgattttc agcaccgtgc cttttttgtg tacgatgaaa
     1681 atatgaaggc ctggctcaat caatacaagt ttatgggaga tatccgacta gcaggcacct
     1741 ttgcatataa atggacgcag ataaaaaaag aatacccgac atacatctat tgtccaatac
     1801 ctttatctga aaaaaggcga acagaaagag gattcaatca agtaagcgag atgttgaaag
     1861 cagcaagaat tccttttcag atgctactca aaagagagaa gcatctgctg gctcaagcaa
     1921 aaaacacgcg aaaggaaaga ttggagatgc ctcaaccttt tgaacttgca gtcacctttt
     1981 ctaagataca aaataaaaaa atattgatta ttgatgatgt ctatacaaca ggacagactc
     2041 ttttccacgc agcagattgt ttgcaggtcg cacagccgaa aatcatccga accatttctt
     2101 tagcaagatg aattagatgt ggaaatttga tagcgattta atttgcaaat aacctagagt
     2161 tcgatataat agaattaaga agagggaaag agtggtcctt ctccttcttg attgtggtag
     2221 acgaaagggg taatttttat gtttagatat aatgtacgcg gcgaaaatat cgaagtaact
     2281 gaagcgattc gcgactacgt tgagaaaaaa gtaggcaaac tagaacgcta tttcagtgac
     2341 gcacccgatg caacggcgca tgtgaactta aaagtttata cagaaaaaac agcaaaagtg
     2401 gaagtaacga tcccattacc ttatcttgta ttacgtgccg aagaaacttc tccagattta
     2461 tacgcaagta tcgatttggt tgttgacaaa ttggaacgcc aaatccgcaa attcaaaaca
     2521 aaaattaatc gtaaatcccg tgaaacgggc atgaatactg cagatgctac tgctttattc
     2581 aatgaagagg acgaaaacga aaatgattct gaattagata tcgtacgtac gaaacgcctt
     2641 tcattgaaac caatggatag tgaagaagca gtcttacaaa tgaacatgct gggacataac
     2701 ttctttattt ttgaagatgc agaaacaaat ggtacaagta tcgtttaccg ccgtaaagat
     2761 ggaaaatacg gactgattga aacagactga tcagccttac ttaaataaca acgaaaactt
     2821 gcagcagcta ctgacagctg ccgcaagttt ttttgagttt cctcctcttt tagatgaaat
     2881 atgaataaat tatgaagttc ttactttaat acgcttaaga tatttcaatt atctataact
     2941 aatgataaaa tgaaaagtaa agtctacata gtctggataa tacacagaag gaaaaggaga
     3001 ttacacaacg catggccaat ttgattcgtt cacttatcga aaacgataag aaagaactaa
     3061 aacgtttaga tagcattgca aaaaaagttg aagcatacgc tgaccaaatg gcagcattaa
     3121 ctgatgaaca attacaggca aaaactgctg aattcaaggg cagataccaa aaaggagaaa
     3181 cattagatca attacttccg gaagcttttg cagtcgtccg agaagcagca aaacgtgtat
     3241 tggggttata tccacaccac gtacaattga tgggtggaat cgtgcttcac gatggaaata
     3301 tccctgaaat gcgtactggt gaaggtaaaa ccttgaccgc aacgatgcca gtatatctaa
     3361 atgctctgac tggcgaaggg gtacacgtag ttaccgtcaa tgaatattta gcaacccgtg
     3421 actcgacgga aatgggcgaa ctttataatt tcttaggatt aacagtgggg ttgaatatca
     3481 actcaaaatc ttcggatgaa aaacgcgagg cttataattg tgatatcact tatagtacga
     3541 ataatgaatt aggatttgac tacttgagag acaatatggt cgtttaccgc catcaaatgg
     3601 tacaacgtcc gctgaattat gccattgtcg atgaagttga ctcaatcttg attgatgaag
     3661 cgagaacgcc gctgatcatt tcagggcaag cagaaaaatc cacagcttta tatacaagag
     3721 cagacaactt cgtgaaacgt ttgaaagaag aagaagatta taaaatcgat gtacaatcaa
     3781 aaacaatcag tttaaccgaa gcagggatcg aaaaagcaga agaaacattc ggtttggata
     3841 acttatacga cattgaaaat actgcattga cccatcactt ggatcaagca ttgagagcca
     3901 actatatcat gcttttagat atcgattatg ttgtccaaga cggaaaagta ctgattgttg
     3961 accagttcac tggtcggatc atggatggac gccgttattc agatggactt caccaagcaa
     4021 tcgaagctaa agaaggcgta gagatcgaag acgaaacgaa aaccatggcg acgatcactt
     4081 tccagaacta tttccgtatg tacaagaaac ttgcgggaat gactggtaca gctaaaacag
     4141 aagaagaaga attcagagaa atttataaca tccaagtata ccagattccg acaaatcgtc
     4201 cggttatccg tgatgatcgt ccagatttgc tttacccaac attacaaagc aagttccacg
     4261 ctgtcgtgca agatatcaaa gatcgctacc gcaaaggtca acctgtgtta gtcggaactg
     4321 ttgcggttga aacatctgag ttattatcag atatgctgaa tcaagaacgt attcctcatg
     4381 aagtgctgaa tgcgaaaaat cactttaaag aagcagagat catcatgaat gctggtcaaa
     4441 aaggtgctgt gacgattgct accaacatgg ctggtcgggg aacagatatc aagttaggac
     4501 ttggggtaag agaacttggt ggtctagcag ttatcgggac agaacgacat gaatctcgtc
     4561 gtatcgataa ccagcttcga ggacgtgcag gacgtcaagg agaccctggt atgtcacagt
     4621 tctacttgtc attggaagat gatttgatga aacgattcgg ttcagaacga atcaaatctt
     4681 tcttagatcg gatgaagatc ggtgaggaag atgctgttat ccaaagcaag atgctgacga
     4741 aacaagtaga atctgcacaa aaacgtgtag aaggaaataa ctatgacaca cgtaaaaacg
     4801 tcttacaata cgatgatgtg atgcgtgaac aacgtgaagt aatctataaa cagcgacaag
     4861 aagtaatcat ggaagaaaaa agcttatcca agccactaat gaacatggtc aaacggacaa
     4921 tcagtcgtgt agtagatgcg catacacaat tagagaaaga aaattggaat ttggaaagta
     4981 tcatcgattt tgccggcagt actcttgtcc atgaagatag tattactctt gcagatatcg
     5041 aaggaaaaac agctcaggag atcaaagagt atctaaacga acgagcacaa gaagtattta
     5101 ataccaaagc ggctcaacta aatggtccag agcaactgtt ggaatttgaa aaagtcgtga
     5161 tcttgcgtgt tgtggactct aaatggacag accatattga tgcaatggat caattacgtc
     5221 agtcaatcgg cttacgtgct tatggacaga acaatccatt agtcgaatat caaacagaag
     5281 gctataagat gtttgaagat atggtaggcg ccatcgagta tgaagtcact cgaatcttta
     5341 tgaaagctga gatccgtcaa aacgtacagc gtgaacaggt ttctcaagga acagcttctc
     5401 actcagaaga cggagatgcc aaagaaaata gtaatactag cgcgaaaaaa cagccaatcc
     5461 atgtgaacaa ggtcggaaga aatgatcctt gtccttgtgg aagcgggaaa aaatataaga
     5521 actgtcatgg aaaaaatgca aactaatttc tgacagcagg attggtgaga caaattcttt
     5581 ttttaagaat tcgtctcacc ttctttctgt tatttgctga ttctgttaga ctagatataa
     5641 tgaaaaaagt atagtgaaag aaggaaaaaa catggaaata tccgaaattc gaaatctcct
     5701 gacagaaatg caggaaaagg tcacaagctt caggaggtct ctttgactta gaccagctgg
     5761 aagaagacat tgcggaagca gaagcaaaaa tggctgaacc tggtttttgg gatgatagcg
     5821 aagctgctca aaaagtgatc agtgaaaaca atgcaaataa agaaacttat gatcaattcc
     5881 attctcttgc tgaagaactg gaagaaatcg aattgatggt ggagatgttg caggaagaac
     5941 ctgatgcagc catgcaagaa gaagcagaaa cacgtatcaa tgcgctagat gaaaaaatga
     6001 agacatatga attatctatg ttgttagatg gtccttatga tcgaaacaac gccatcgttg
     6061 aattgcatcc aggggctggc ggaacggagt cccaagattg gggcagtatg cttttacgta
     6121 tgtatacacg ctgggcagag cagcatgggt atcaagtaga gacactagat tatcaagcag
     6181 gtgatgaagc aggaatcaag agtgtgaccc tactgatcaa aggacacaac gcatttggtt
     6241 atctgaaatc agaaaaaggt gttcatcgcc ttgtacgtat ctcgcctttt gactcagcga
     6301 aacgacgtca tacttctttt tgttcggtag atgtgatgcc agagcttgat gaaacgatcg
     6361 atatagaaat caatccagat gatttgaaaa tcgatacgta ccgagcaagt ggtgcaggtg
     6421 gtcagcacat caataaaaca gaatctgctg tacggatcac tcacttgcct acaggaacag
     6481 tagtagctag tcaggcacaa cgttcacaat tgaaaaaccg tgagcaagcg atggggatgt
     6541 taaaagctaa gctttatcaa ttagaagtgg aaaagaaaga acaagaagca gctgcacttc
     6601 gaggagaaca aaaagaaatc ggctggggtt cacagatccg ttcttatgtt ttccatccat
     6661 attctatggt aaaagaccat cggacaaacg tagagacagg aaatgtccaa gcagttatgg
     6721 acggtgacct tgatttattt attgacggct atttaaaaat gcatctacaa taagaaccca
     6781 ttgttaaatt gaaataaaat tgtaagattt tgcaagcatg ttgaaacatt gccatgctat
     6841 aatagctaaa gactgaaaaa gatatggaga gaacgccatg attgaaatga aggatgtaat
     6901 gaagaagtac tcaaatggta cgaccgctat ccgcaatctc tcaattgtca tcgaacaagg
     6961 agagtttgtc tatgtggttg gtccgtctgg agccggtaaa tctacattca tcaaattgat
     7021 gtatcgagaa gaaaaagctt caaaaggaac tttgaacgtc gcaggtcatg atctaatagc
     7081 aatcaaaaac cgcgaagttc cttatctacg cagagaaatc ggcgtggtgt tccaagacta
     7141 taaattatta ccaagaaaaa cagtatatga aaatgttgcg tacgctatgc aagttatcgg
     7201 cagaaaacct cgggatatca aaaaacgagt aatggaagtg ctggaccttg ttggtttgaa
     7261 acacaaagtt cgagtctttc ctagcgaatt atccggtggg gaacagcaac gtgtctctat
     7321 tgctcgggca atagtgaata cgcctaaagt acttatcgcg gatgaaccga caggaaatct
     7381 ggatccagaa aactcatggg aaatcatgaa attattagat cggatcaatg cccaaggaac
     7441 tactgtcgtg atggctacac ataacagtac gatcgtaaat acgattcggc accgagtaat
     7501 tgctatcgaa aacggccgca ttattcgaga tcaagcggaa ggggaatatg gatacgatga
     7561 ttagaacctt tttccgccat ttattagaaa gtatcaagag tttgaaacgt aacggctgga
     7621 tgacagttgc ttcagtcagt gcggtaacga tcacattgac tttagtcggt gtattcatgg
     7681 cagttatcat gaacgcaact aaattagctc aagatataga aggaaacgtt gatgtctctg
     7741 tattcgtaga tatcggtaca aaagacgaag agatgaaaaa gctagaaaaa gaattgaatg
     7801 acttagatca tgttaaaaaa gttgaatttt caagcaaaga acaggaattg aaaaaaatcc
     7861 aaaacgaaat gggtgattcc tggaaattgt ttgaaggtga caataacccg ttatatgatg
     7921 tgtatgtagt aagtgctact gatccttctt atacaaaaca agtagcagaa gaagcagcag
     7981 atttgaagaa tgtttctcgt tctgattacg gtggagcttc ctcagatcgg attttccaaa
     8041 tcgctggtgc agttcggaca tggggattag ttgcagcggc attgctgctg ttcgtagcga
     8101 tgtttctcat ttcgaacacg attcggatca cgattttgtc tagacagcgt gaaattcaaa
     8161 tcatgcgttt agtcggtgca aaaaatggat atattcgctg gccgtttttc cttgaaggtg
     8221 gctggatcgg tctgatcggt gcgattcttc cgattttgat catcacattt ggttatgcat
     8281 ggtttttcca attgatcaat ccatcattat tacgttccca ttattcattg attcatccac
     8341 aaaatatcgt ttggaaaatc aatcttttaa tggtcggcat tggtgtgatc attggttcac
     8401 ttggctcgat catttctatg agacgtttct tgaaattcta ataccacaat ataaataaaa
     8461 tgagaaagtt ggacagtttc gttccagctt tcttttttta ttggctagga gaatagaagg
     8521 aagcggcttt tttcccttta ctttaaaaaa caacttgggt attcttaagt tggtagaaga
     8581 agaggtgaag ccgtgaaaat tatcgttgac aattttttta cgatcctttt gattatgaat
     8641 attttgcttt cctttatcat cgtttttcga gaaagaaagg aaactgctca aacatgggca
     8701 tgggtgctcg ttttgatgtt cattccagtt gtaggattta ttttatatat ttttttagga
     8761 agagggattt ccaaagagaa aatctttgat ttgaagatcc aagggaaaat cggtaagaac
     8821 ctggaaattg aagaagacaa acaggcattg atgcgagggc tttatcctca tccgcctact
     8881 ggacaagtag atgtgaaaca attgatctat atgctcacgg tatttgaaag tacgctctat
     8941 acgactaaaa atgaaatcac tttatttaca gatggtcgtg aaaaattcga tgcgctgatt
     9001 gaagatatca agcaggcaga agaccatatc cacttccagt attatattta tcgcagtgat
     9061 gcattaggtg aagaagttcg agatgcattg acagaggcgg caagacgagg gggaaaagtc
     9121 agagtccttc ttgatgcatg gggttccaca caagtttctt ccagcttttt tcagaatttg
     9181 aaaaaagcag gcggagaagt cgcatttttc tttcctctat ttgttccgta tattaatcct
     9241 cggatcaatt atcgcaatca tcgtaaaatc gtagttattg atgggaaaat cggatataca
     9301 ggtgggttca atgttggaaa tgaatatcta ggattggtaa agaaatttgg ttattggcga
     9361 gacaatcatt tgcgtattta cggagaagcg gtgtatacct tgcaaaaccg ttttttgatg
     9421 gactggaatt ctcagcatac aaaagaagta ggctattctc ccaaattttt tccgtccatt
     9481 cactctactg gtgatatagc tgttcaaatc gtgacaagcg gaccagatac cgaacatgaa
     9541 caaatcaaaa tgacttattt gaaaatgatt tcgttagcca aacgggagat attgatccaa
     9601 acgccttatt acataccgga tggatcgatt catgaagcac tgaagttggc tcttctttca
     9661 ggcgtaaaag ttcatatcca aatacctaac aaaccagatc atcttctcgt ttactgggca
     9721 acgtattctt tcgctgcaga gctaatcgag tatggagcaa ggatcgaaac atatgagaat
     9781 ggatttattc atgcaaagac gatgattatc gatggtggca ttgtttcagt tggctcagca
     9841 aatatcgatg ttcgttcatt tcgcttggac tttgaggtca atacactggt ttatgatgaa
     9901 cgaatggctt cacgtgtaag aaaagctttt tttgaagact cgaaaatttc tacgcatctc
     9961 acaaaagaaa tgtatgaaaa ccgaggaata atcatcaaga tgaaagaagg attagcaaga
    10021 ttgatttcgc cgctccttta aaacataaaa aggacatgag aaccatcaac agatggagat
    10081 ctcgtgtcct tttctaacgg ggccatttct gagagtacgt cagaaatgga gaaagcgtct
    10141 cgcatgagcg aaattaactt tggggtatct cttgtttttt ggggaaagca agaaataagc
    10201 taatatatgc tattaaaaga gacacatttc aaagccagta tagcatagaa acttttaatg
    10261 agatagtttt ttttacaatt atgagaattt attttgttat ttaatgattt tgattcgttt
    10321 tcatcaagta atgaaaatga taaaataaac atactttagg attaaaggag atttctaatg
    10381 aaaaaactta aattattctt accaataata ggattagcta ttcttttatc aggctgttca
    10441 aagtggattg atcgaggaga atccattacg gctgttggct cttctgcttt acagccttta
    10501 gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg gaaaattcat caatgtacaa
    10561 gggggcggaa gcggaactgg tttgtctcaa gtacagtcag gagcagtcga catcggaaac
    10621 tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct cagcattagt cgatcatcga
    10681 gtagcagttg ttgggatcac tcctattgtg aacaaggata ttggcgtaaa agacatcagt
    10741 atggaaaacc tgaaaaaact atttacaggc gagatcaaaa actggagtga agtcggtgga
    10801 aaagatcagc cagtagttat attgaaccgc gcttcaggaa gcggaacccg tagcacgttt
    10861 gaaaaatggg tattggacgg agaaacagct gttcaggcac aagaacaaga ttcaagcgga
    10921 atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca gctatgttgc tttttcttat
    10981 gtttccgatg atgtagatac gttgaatgtt aacggtgtag aaccgacaga tgaaaatgtg
    11041 acaacgaaca aatggacgat ttgggcatat gagcatatgt acacaagaga tcatccaaaa
    11101 aaactgacaa aagaattttt agactatatc ttatctgatg aagtacaaga taacatcgtt
    11161 ggagaactag gttatatccc tgtttctcat atgaaagtcg aacgtgattg gcaaggaaat
    11221 atcgttaaag agtaatcttt acacaaaatt tacaaactta gctaaacttt tacatgttat
    11281 taaaaccaaa ttgatgttca atttgtacac tgagtgagta tgttaaacct gattaagggg
    11341 gagtcatctg tggaagatgt gaaaaaagta ttattaacaa aatcaaaacg ttcaaaaatg
    11401 gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc tgatcgtgtt ggttgtatta
    11461 tctatttttt attttgtagc tagtaaagga ttggccacct tttttactga taagatcaat
    11521 gtatttgatt ttctgtttgg tacagattgg aatccaagcc aaatcggagc aaatgggaaa
    11581 ccaatggtag gggcattgcc tatgatcaca ggttcgttta ttgtgacctt tttatctgct
    11641 atcgttgcca cgccatttgc cattggtgca gcagtattca tgacggagat ctctcctaaa
    11701 aacggaacga agatcttaca gccggtcatc gagctgcttg taggtattcc ttctgtcgtt
    11761 tatggtttta tcggtttatc agttatcgtg ccttttgtca gaagtatttt tggcgggact
    11821 gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc gactgtcaca
    11881 acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc ttcattagcg
    11941 cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg
    12001 attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt agctatccaa
    12061 atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc ttctacattg
    12121 acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga aaataatgtg
    12181 ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat cgtgatccgt
    12241 atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat aaaattgcta
    12301 caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct ttactacttt
    12361 atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa ccttccaaag
    12421 cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat ttgcttttga
    12481 tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt tcagaatatg
    12541 ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg agctcgttgc
    12601 cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc cagtatggtt
    12661 tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt ttgactagaa
    12721 cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga ttggccttag
    12781 gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca ccagggattt
    12841 tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca ttgatctata
    12901 cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta agtgtttcaa
    12961 gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg aagatcaata
    13021 cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc gtgttgatta
    13081 ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa
    13141 tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt
    13201 catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac atgtctggta
    13261 tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata agatcactgc
    13321 actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa
    13381 tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg tagatattaa
    13441 tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt ttcaacgtcc
    13501 taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac agcacggcga
    13561 aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg
    13621 ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg ggcaacagca
    13681 acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc tagacgagcc
    13741 ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg taaacttgaa
    13801 agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc gtatcagcga
    13861 ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta acaagatatt
    13921 taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg
    13981 aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt attatggaaa
    14041 gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca ctgcgatgat
    14101 cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga tgaatgacct
    14161 gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca tctatggtcc
    14221 aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc agccaaaccc
    14281 atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag gggaaaaaga
    14341 taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg tttgggatga
    14401 tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt
    14461 atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg aaccaacaag
    14521 tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat taagagagcg
    14581 gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt cagataagac
    14641 ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga tattcatgaa
    14701 tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag gaggaaacgg
    14761 aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt tctacgaaat
    14821 ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca aacacgataa
    14881 aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg aaacacgctt
    14941 ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg atttgcgtat
    15001 gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc atgcggtgtc
    15061 tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga
    15121 gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt
    15181 caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag tcaatgacta
    15241 tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg agacagtcat
    15301 cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag actatgtgac
    15361 aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt taaatacgaa
    15421 ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg aaaagaggtt
    15481 gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa
    15541 aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca agcttttgaa
    15601 caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt tcatcatttc
    15661 atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt ttatttgtgt
    15721 gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg tcatcgagat
    15781 agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga tgaacacgga
    15841 tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa tagaaacagc
    15901 aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat ggcagctagt
    15961 agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg gcgccatgtt
    16021 gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca atatcgtttt
    16081 acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat taaatatatc
    16141 tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg gatggaatag
    16201 aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat tttttatttg
    16261 tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac tgaattatct
    16321 gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag ctggaggaac
    16381 actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc gacaaccaga
    16441 aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg tgattggctc
    16501 gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc ttcttttata
    16561 tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat tggaattttt
    16621 acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg tcctcttttt
    16681 tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg gattttagca
    16741 atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg cttattcatc
    16801 aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc gatactggca
    16861 ttgtcggtca taggattttt attgattgct gtggttatct tattttttat caacggttat
    16921 aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg tttcctacta
    16981 ttgcttccta tcactgattt tttagcaatc caaggtttta catcggtaga actagaagaa
    17041 ttatctggct tagataagac tttagcgatt gattttagat tacttacgag atcgttgctt
    17101 ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag tgggacattc
    17161 gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc tagtttcgct
    17221 attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt catgggaagt
    17281 tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca agtgatcaat
    17341 gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc cgcactactt
    17401 gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa agaacaactc
    17461 aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc agtgcataat
    17521 atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc catgaaagaa
    17581 agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga aggcttagat
    17641 ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga agcagacaag
    17701 gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa acttgaaaac
    17761 ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca agatcaagaa
    17821 aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc ggcgcgtcta
    17881 gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa acaggaagca
    17941 ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga attagcacag
    18001 cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac actgttagaa
    18061 gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg gtctgagaaa
    18121 aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca agcaacagat
    18181 acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg caaattcttg
    18241 aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg gaaagatgtc
    18301 agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta ctatgaagct
    18361 cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt aaaaacgtgg
    18421 gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat gggtgctgaa
    18481 ccatttgaag cattctctga acggagccag attgaggtta atgaagatca tatctccttc
    18541 caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa acgagtatac
    18601 gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt ggaagctaaa
    18661 gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac tgcaacgatg
    18721 ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat cttagattct
    18781 attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa cctttctgtg
    18841 tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa aaaagtagaa
    18901 gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg tttggaagga
    18961 catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga agtagttcgt
    19021 gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga tggtgaaatc
    19081 gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac agataaataa
    19141 agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt aaaatagctt
    19201 cccctatttt acaaaattgt gccttattgc cgatagtcta tctcttgaac accgtttacc
    19261 aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca gaaaaagctc
    19321 gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga caccgattaa
    19381 gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg tgagacatga
    19441 tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt gacagggact ttagcaggaa
    19501 taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta ttcctgagct
    19561 tagttgcttt tggaagtcca attattttat acattttatt agctttgatt atcccatctg
    19621 cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa aacaattatc
    19681 gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc gatgatgacg
    19741 aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt taacattcat
    19801 atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga cggccgttct
    19861 tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga cgatactgtc
    19921 cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg cagcgattct
    19981 taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat tcggtgcagc
    20041 gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac ataacagtag
    20101 aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga atcaggaatg
    20161 cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta gtacttttgg
    20221 ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca tgaatgtcag
    20281 aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg acaggcgacg
    20341 agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga ctagagttga
    20401 ctggttattt taattattat tcccataatc gtttacagct atttggcagt aaagaaatca
    20461 gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga ttatgtagtg
    20521 aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa atgatccagg
    20581 cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct cgtttattag
    20641 gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc cacggtgttt
    20701 tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata ggaaaaagtg
    20761 aaacagcgtt ggagttgatc aaacgcggcc atcgattgat cgcagacgat cgagtagatg
    20821 tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg gaacatctga
    20881 ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg agtgcggtac
    20941 gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa gataagaaat
    21001 acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc attccacaaa
    21061 taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta gccgcgatga
    21121 atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa agattaagtc
    21181 gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt tagcacaaat
    21241 caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat tgatcatcgt
    21301 atcaggtatc attatcgcta tgtggcttag cagcagagag gctgttcggg taggattaaa
    21361 agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta ttggcgctcg
    21421 cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc aaatattcaa
    21481 tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga tcacgctgta
    21541 tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg ctgcaccaag
    21601 tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg aagcatatgg
    21661 accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca tcattgataa
    21721 tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac tttggagttt
    21781 gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga aaaaaggcga
    21841 agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg aaggattacg
    21901 tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat ctgctgtgtt
    21961 atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt taccggatta
    22021 tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt aaaaatgaaa
    22081 aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca ggctcttggg
    22141 gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt tggggacatc
    22201 atgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat ttaccagatc
    22261 attatctacc cgattccatc caagcattca ccaatatgga agatgcagta aaagacgcag
    22321 atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa gaactagtgc
    22381 ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa cagggaacac
    22441 ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga aaagctgttg
    22501 ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc actacgatca
    22561 cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc atgaataatt
    22621 attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca gctttgaaaa
    22681 atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat aatgcaaagg
    22741 cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca atgggtgcca
    22801 acccgctgac ctttatcgga ttaagcggaa taggcgatct agtcgttact tgcacaagtg
    22861 ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc ctagaagagg
    22921 tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca gcgatggaac
    22981 tagcccaaat gatggaaatc gaaatgccca tcactcaaac aatctatgaa gtgctgtatg
    23041 aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatgga aaaaaagaaa
    23101 atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtta aaaaagcagt
    23161 tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta tggcaaagga
    23221 aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag cgttgaaatc
    23281 aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg aagatcactt
    23341 tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg aattattgaa
    23401 attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc atccaaaggg
    23461 tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat ttgtcgttat
    23521 gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattga tcgatgacta
    23581 tgagcgtaca cacgcttcta cgattgccgt tatgcaagtt cctcacgatg aaacttcaaa
    23641 atacggaatc atcaacccag gggaagtact ggaagatggg ttatacaatg tcaaaaactt
    23701 tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg ggcgttactt
    23761 attgactcca gaaatctttg atgtattatc agaacaacaa ccaggtgctg gaaatgagat
    23821 ccaactaact gacgcaatcg atacgttgaa taaaacacaa cgcgtatttg cccgtaaatt
    23881 cacaggtaaa cgttatgacg ttggtgacaa atttggtttt atgaaaacaa gtattgaata
    23941 tggtctgaca catccagaag taggggcacc tttgcgtgaa tacatcatgt ctttaggtaa
    24001 aacattagaa gcagaaggaa gcaagatcaa tcataatcca aaaggaataa cagaaatcaa
    24061 agaagaaaag aaaaaataat ctttttttct aacgagcttc agcggctaat tgaataaaaa
    24121 gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct tatagaaaag
    24181 agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg atcgcagcaa
    24241 tcgcctttgc gatacttgtc gttttcatcg ttcgcgtatt gttgcaagtt tccaaaactt
    24301 tagaaaaagt agaccagact gtcgcagaag caaatacgac aattgagatc gtcacacaag
    24361 acgtagatct tttatctaga caagtagaag gcttgctagt caaaagtaat gagctgctct
    24421 tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt gctgatttaa
    24481 gtgaaagtgt atcggaactt aatcattcta gtaaaaacat cgtgacgaaa gtcggcggaa
    24541 tcggaaaaac gacagctaaa gcgactgttg ccggaaaagt cggtggaaca gcgatgaaat
    24601 tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataagaatgg ctaaaaaagg
    24661 tggattttta ttaggtgcag tagttggagg tacagccgca gcaatcgccg cattattgtt
    24721 agcacccaaa tctggtaaag aacttcggga tgaattggct gctcaagcag atgatttgat
    24781 ggacatggct tcagattaca cagatatggc aaaagaaaaa tcatctgagt taacaggtat
    24841 tgcaaaagaa aaagcgcaag gtctttctca acaagcaggg gatctagcag gcaatgttaa
    24901 gcagaaggta agtgaatcag cagaggaagc aggaagtgaa tcagatgata tcttgttaaa
    24961 cctcaaagaa caatcagctg atttatctaa tcgtttcaaa aaaacagcag aagatctgaa
    25021 aaagccagca gacgaattag gtagtattgc taaagatact tcagaagata tcatgatcga
    25081 tgtaaaagat gctgcagata aagcaggaga gacggtcaat gaaggaatgt cagaagcaaa
    25141 accagttgca gatgacacaa aagaaatgtt gaaaatggca aaagaagatg caaaagaagc
    25201 taaaaaagaa atgaaagata caatgacaga ttcgactacc aatcactaag attactcatt
    25261 tctagaataa aaaaggtgag agcggaccag ctctcacctt ttttattttc attactctaa
    25321 aacaagtaat tcttttggtg tagtcgtgaa tggcacacaa ccatcacttg tcacatacac
    25381 acaatcttcg atacgtaccc ctactttttc aggaatatag attcctggtt caagagagaa
    25441 gcacatccct tcttcaatca ctaagtcgtt tccttctaca agagaaggga attcgtggac
    25501 ggttgttccg attccgtgac ctaaacggtg attgaaatat tcgccgtagc catagctgtt
    25561 gatcacgttt cgtgcgattt gatcaagttc acctgctgtc acacctgggc gaactgcctc
    25621 ttgtgcggca agctgtgctt caagagtgat gttatagatt ttttcttgga actcagttgg
    25681 tttttgataa gcaactgtac gagtagcatc actgcaatag ccattccaaa taacgccaag
    25741 atcgaataac actagttcat ttggtgaaac cttcgtgctt cctggtgtac catgagggct
    25801 tgcagcattt gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa caccttgttt
    25861 ttttaattgg tattcgattt ctgcgataat tgcttgttca gttacgcctt cttttactgt
    25921 tttgaagcca atttcaaagg caacatcggc ccatttacca gcttcgatca gtttgtcttt
    25981 ttcttctacc gttttgatca gttgcagacg ctggataata gaagttagat cggtgtaaga
    26041 atctgtacct gtcaagatat cagagatagc atcgaagcga tcgacagtca gagcattctt
    26101 ttcaatacct acattaccag gttgcccata gcgtgcgcga agctcttttc cgatgatctc
    26161 ccaaggattt tcgctgtcta agtagccacg tacatcgtac gcccagctgc tgttttgagc
    26221 atcttcaact tccagagcag gagtgaaaag gaaaggatct tgttccagag ggataaaaag
    26281 agctagtaca cgctcgtgag gatcactttc atatccggaa aagtaagcga tgtggccagg
    26341 gtcgctgata taagccaatt ctacttggtg ttgtgccatc cattgacgta gttcattgat
    26401 ttttacgtta ttcatttggc aacctacttt ctaaattaat tttgtattca ataattgtac
    26461 cactgattga ttaaaagacc aacgttctta aaactgaaaa aagtaaaaat tttcatgaaa
    26521 acagagataa aacggttgca attgttgaga ttatttgata tcctatttta gaaatgtaag
    26581 acgtattttc agaaaacata taaaatatga ataggagaac acaaatggaa aagcaaacaa
    26641 tcactatcta tgatgtagcg agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg
    26701 taaatggcaa tccaaacgtc aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc
    26761 gcttggatta tcgtccaaat gcagtcgcac gtggcttggc aagtaagaaa acaacaacag
    26821 ttggggtaat cattcctgat gtcagcaata tgttctttgc ctctttagca cgtggtatcg
    26881 acgatgtggc aacaatgtac aaatacaata tcatcttagc aaactcagat ggcaacgatc
    26941 aaaaagaagt caatgtattg aataatttat tggctaagca agtggacggc gtgatcttta
    27001 tgggtcacca tatcacagac gaaatccgtg gagaattttc acgttcaaaa acgcctgttg
    27061 tactagctgg ttccattgat ccagatgaac aagtaggtag cgtaaacatt gattatgtgt
    27121 cagcaacaaa agatgcagta aataaattag caaaaagcgg caataaaaaa atcgcttttg
    27181 tcagcggtgc gctgattgat cctatcaatg gtcaaaaccg tctaaaaggc tataaagaag
    27241 cattaagcga aaacaatcta ccatatagcg aaggattgat ttttgaggca caatacaact
    27301 ttaaagatgg tctgtctcta gttgaccgga tccataatag cggtgcaaca gctgtctatg
    27361 tttcagacga tgaattagcc attggtatct tggatggttt attagaccgc ggcgtaaaag
    27421 taccagaaga attcgaaatc atcacaagca acaattcttt actgacagaa gtagctcgtc
    27481 ctcgtttgac aagtatcaca caaccattat acgatttagg agcggtatcc atgcgcctat
    27541 tgacgaaaat gatgaataaa gaagaagtcg aagaaaaaac gattatcttg ccttatggaa
    27601 tcgaagaaaa aggatcaact aaataataaa agatcaatca tttaaaggtt gttccaaaag
    27661 tggtcaactt ctggaacaac gtttatacga gaaagggggt tatgacaaaa ttttgtcata
    27721 accccttttt ttttgtgact ttaaaaaaag agaaaatggc ggaaacaaac cttacgtcca
    27781 tcgtatacga cacaatctgt gcgtagatgc tacattttgt tcactactat caaaattact
    27841 ttaaaatgaa aacgtgataa aaaagaaagt agaaaagtag ataagtaact ctaaaaaatc
    27901 caaaaaaatc caggctcgat tgcgtactta tcttaaagga gaaacaatcc atcatatata
    27961 acccgaaagg agacttctat ttgcctaaga aaagacgaaa gaccatagtc atcattttga
    28021 ccattcttac tataggaatc ggagggaaat tttacatgga caaaagagaa atgcaaagac
    28081 agaaagattt actcgcagta gaaaaacagt ccgtcaaagt tctgaaaaat acctttgcgg
    28141 atatcaagga agtgaagatt gaaaaatctg ctcgaaatga aatgacgggt tcataccgga
    28201 tagttattct aatgactaat aaacaagatc aatcgattta tttttcttat agtttttgga
    28261 aagaaagaaa tgagatagga agttacggaa tagttgatga aaaaaagcaa aaagaaggaa
    28321 atacgctcaa taaagttaaa gtaacttact ctaatggaaa tgaggaaagt atttaatgat
    28381 tactgacaaa caatatagtg aattaagtga tgctgtttac tggctagatc ctaaacatag
    28441 agactatgta cctgaaatgc aagaaaattt aagttttaaa attaacggca ctatgtataa
    28501 aatcctaaaa atcaaaaact ccttcgacgg catgcaggcg atggctgtag cgccaatcgt
    28561 ccatagcaaa ttggagaaaa atttcaaaaa taaaaaaatt cctgcaaact ttcgcgtact
    28621 taaataatga aggtgtcaat acttacataa aaagaaagga ggcacgttct tgtcaaggaa
    28681 aataagaaaa ctcagcttag ccattttatt aatcgtcata ttagggatcg gagggaaagt
    28741 ctatatggac aaaagagaag cacaaaggca gaaagattta ctcgcagtag aaaaacagtc
    28801 agtcaaagtt ctgaaaaata cctttgcgga tatcagggaa gtgaaggtag aagaatttaa
    28861 aaaaaaccct gttaccggaa gttatggtgc acttgtgaca atgacaaata atgaagggaa
    28921 atctgtttat tttgattata gtttttggaa ggagagaaat gaacttggtg gttttggcat
    28981 tgagaacaga gcggtacaaa agacaggagt cactataaaa agagtaaaag taattttttc
    29041 taacggacag gaggagttag tatgacaata aacgataagc attacaatga tattagtgag
    29101 aaggtttatt ggctagatcc caaatatccc cgctacaatg agggatacaa aaagaactct
    29161 gttaaagaat ttgctggtat ggaatttcaa atcctacaaa tcaaagactc attggacggc
    29221 atgcaggcga tgtctgtagc gccaatcaag aacggtaaag tggatctttc tcaagtagtg
    29281 attgcttttg caggaacgaa cttaagtgat tggaaagatt tggaaacgga tgcaagaagt
    29341 atcatgaatc tctacgggaa tagaccagga gcaagttttt ctgaaaaagc aacaacaaac
    29401 aagattttat ccgttagtgg attgaaaaca gcggggcaaa tcaattcagc gattgcattt
    29461 gctaatgaag taaaaaagga atatggagaa gctgctattt ctactaccgg ccattcatta
    29521 ggtggattct tagcattata tgtagcagca gagaaacaat ggaaaaatgt cggcttcaac
    29581 gggccggacc cgtatgatct cttatcacca gaagcaaaaa aatgggtgga agaaaatccc
    29641 ggtatgctca ccaattaccg aaatcacgcc gattttatcg gtaattttgg tggtaatggc
    29701 acaggtgctg aagttcggat cagtatggaa acagcattct tgaatctgct tgcttcccac
    29761 agtttaacct cttggaagtt tgatgaaaat ggaaagctga ttattcctga aaatgaaaac
    29821 aatgttaaag ctcgaaaaca acagaaagaa aaccaagcga cccagttgtt ttcctatcag
    29881 ttgcaagaac tgaacaacat acgtaaaaaa ctacgattaa gtggcggcca gctgacagtt
    29941 aatgaacaaa tttacttaga tgacacacaa gctaggttag tagtggaaaa gttatcggca
    30001 aaaatgaaaa gcgctatgga agaagttatc cgcatcaatc aaagaggaat ttttgagcta
    30061 gagaaaaaat ggcaagaagg cttgcggcaa gcacgtgttg tagccccttc gttgactgaa
    30121 caggaaatca tagaagcatt agctagtgtg ggcgccacaa aagaaaactt agtcgattct
    30181 gcgaaagaag tctatctgct acgaatcaat aaggcaagac gtatggggga ggcctttgat
    30241 acattggcga aggagatcca agacaaaatc aatgaaatcg tggcacgaga tcgagaatta
    30301 gcacagcaat tcaattaaag gagaagtaga tgaaactaga agaattggaa agagattatc
    30361 gaagtgaacg aagacaatta gaagctcaag aagatgaatt gatccacttt aagaatcaag
    30421 gattacgatt gatggagcaa gaggagcagc gactgcatca tgtattaaag aacttagctt
    30481 tagataatga gcctctgttt caagcaagac gaagttattc gaattatcag gagcattatc
    30541 aacaagccct acaagtagaa cgacgaaaac tctatcagca gcaagatgaa ctagaacgtt
    30601 cctatcgtca aaaaatcaga caaataacta tggagaaatc atcttatgaa tgaattttta
    30661 atgcaattac taaaagaatt agagaaaaat ctacatcgat caaatgtacg ttcagttggt
    30721 atggggatat ctcagcaaat ttgggcagaa aaacgattga cagatcaaaa ctaccaaatg
    30781 actgcagaag aagctgctca agaattttct aaccgacatg tacacttgaa aggatcagta
    30841 aaagggcaat ttgcagaaaa actaactgaa ttattccaaa gacaagaaaa gaaattaaaa
    30901 cagtttcgtt agtaaagtta tatagaaaaa ttttgtgaaa atggttt
//
LOCUS       DACETN010000025        29110 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DACETN010000025 DACETN010000000
VERSION     DACETN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.026"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..1031)
                     /locus_tag="IXF98_001036"
     CDS             complement(<1..1031)
                     /locus_tag="IXF98_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1555366.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IXF98_001037"
     CDS             complement(1113..1802)
                     /locus_tag="IXF98_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1555367.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXF98_001038"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXF98_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1555368.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IXF98_001039"
     CDS             complement(2329..2832)
                     /locus_tag="IXF98_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1555369.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXF98_001040"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXF98_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1555370.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXF98_001041"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXF98_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1555371.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXF98_001042"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXF98_001042"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1555372.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXF98_001043"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXF98_001043"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1555373.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IXF98_001044"
     CDS             complement(6444..7463)
                     /locus_tag="IXF98_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1555374.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IXF98_001045"
     CDS             complement(7636..8523)
                     /locus_tag="IXF98_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1555375.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXF98_001046"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXF98_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1555376.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXF98_001047"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXF98_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1555377.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXF98_001048"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXF98_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1555378.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IXF98_001049"
     CDS             9754..10398
                     /locus_tag="IXF98_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1555379.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IXF98_001050"
     CDS             complement(10454..10957)
                     /locus_tag="IXF98_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1555380.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IXF98_001051"
     CDS             11554..12519
                     /locus_tag="IXF98_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1555381.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IXF98_001052"
     CDS             complement(12618..13790)
                     /locus_tag="IXF98_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555382.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IXF98_001053"
     CDS             complement(13777..14922)
                     /locus_tag="IXF98_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555383.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IXF98_001054"
     CDS             complement(14936..15868)
                     /locus_tag="IXF98_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555384.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IXF98_001055"
     CDS             15985..17064
                     /locus_tag="IXF98_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1555385.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGISPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IXF98_001056"
     CDS             17061..17690
                     /locus_tag="IXF98_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555386.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IXF98_001057"
     CDS             complement(17800..18027)
                     /locus_tag="IXF98_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1555387.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IXF98_001058"
     CDS             complement(18130..20718)
                     /locus_tag="IXF98_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1555388.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IXF98_001059"
     CDS             complement(20738..21397)
                     /locus_tag="IXF98_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1555389.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXF98_001060"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXF98_001060"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1555390.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IXF98_001061"
     CDS             complement(22170..23036)
                     /locus_tag="IXF98_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1555391.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IXF98_001062"
     CDS             23145..23786
                     /locus_tag="IXF98_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1555392.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXF98_001063"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXF98_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1555393.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IXF98_001064"
     CDS             complement(25227..26126)
                     /locus_tag="IXF98_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1555394.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IXF98_001065"
     CDS             complement(26128..26925)
                     /locus_tag="IXF98_001065"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1555395.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IXF98_001066"
     CDS             complement(26903..27607)
                     /locus_tag="IXF98_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1555396.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IXF98_001067"
     CDS             27738..28313
                     /locus_tag="IXF98_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1555397.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IXF98_001068"
     CDS             28431..29108
                     /locus_tag="IXF98_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1555398.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6168 c         5232 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcatc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa
//
LOCUS       DACETN010000026        28249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DACETN010000026 DACETN010000000
VERSION     DACETN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.027"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(29..826)
                     /locus_tag="IXF98_001069"
     CDS             complement(29..826)
                     /locus_tag="IXF98_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1555399.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(823..2007)
                     /locus_tag="IXF98_001070"
     CDS             complement(823..2007)
                     /locus_tag="IXF98_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1555400.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2032..3279)
                     /locus_tag="IXF98_001071"
     CDS             complement(2032..3279)
                     /locus_tag="IXF98_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1555401.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3494..5239
                     /gene="dnaX"
                     /locus_tag="IXF98_001072"
     CDS             3494..5239
                     /gene="dnaX"
                     /locus_tag="IXF98_001072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1555402.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5259..5573
                     /locus_tag="IXF98_001073"
     CDS             5259..5573
                     /locus_tag="IXF98_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1555403.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5598..6194
                     /gene="recR"
                     /locus_tag="IXF98_001074"
     CDS             5598..6194
                     /gene="recR"
                     /locus_tag="IXF98_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1555404.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6258..6902
                     /locus_tag="IXF98_001075"
     CDS             6258..6902
                     /locus_tag="IXF98_001075"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1555405.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6917..7246
                     /locus_tag="IXF98_001076"
     CDS             6917..7246
                     /locus_tag="IXF98_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555406.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQL"
     gene            7260..8198
                     /gene="holB"
                     /locus_tag="IXF98_001077"
     CDS             7260..8198
                     /gene="holB"
                     /locus_tag="IXF98_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1555407.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8234..9058
                     /locus_tag="IXF98_001078"
     CDS             8234..9058
                     /locus_tag="IXF98_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1555408.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9051..9398
                     /locus_tag="IXF98_001079"
     CDS             9051..9398
                     /locus_tag="IXF98_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1555409.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9467..10339
                     /gene="rsmI"
                     /locus_tag="IXF98_001080"
     CDS             9467..10339
                     /gene="rsmI"
                     /locus_tag="IXF98_001080"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1555410.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10448..11569
                     /gene="dinB"
                     /locus_tag="IXF98_001081"
     CDS             10448..11569
                     /gene="dinB"
                     /locus_tag="IXF98_001081"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1555411.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11623..12225)
                     /gene="nrdG"
                     /locus_tag="IXF98_001082"
     CDS             complement(11623..12225)
                     /gene="nrdG"
                     /locus_tag="IXF98_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1555412.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12540..14729)
                     /gene="nrdD"
                     /locus_tag="IXF98_001083"
     CDS             complement(12540..14729)
                     /gene="nrdD"
                     /locus_tag="IXF98_001083"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1555413.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15055..15822
                     /locus_tag="IXF98_001084"
     CDS             15055..15822
                     /locus_tag="IXF98_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555414.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15812..17854
                     /locus_tag="IXF98_001085"
     CDS             15812..17854
                     /locus_tag="IXF98_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555415.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18135..18284
                     /locus_tag="IXF98_001086"
     CDS             18135..18284
                     /locus_tag="IXF98_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1555416.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18295..19809
                     /gene="dltA"
                     /locus_tag="IXF98_001087"
     CDS             18295..19809
                     /gene="dltA"
                     /locus_tag="IXF98_001087"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1555417.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19806..21011
                     /gene="dltB"
                     /locus_tag="IXF98_001088"
     CDS             19806..21011
                     /gene="dltB"
                     /locus_tag="IXF98_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1555418.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21056..21289
                     /gene="dltC"
                     /locus_tag="IXF98_001089"
     CDS             21056..21289
                     /gene="dltC"
                     /locus_tag="IXF98_001089"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1555419.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21292..22560
                     /gene="dltD"
                     /locus_tag="IXF98_001090"
     CDS             21292..22560
                     /gene="dltD"
                     /locus_tag="IXF98_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1555420.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22701..23783
                     /locus_tag="IXF98_001091"
     CDS             22701..23783
                     /locus_tag="IXF98_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1555421.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23783..24586
                     /locus_tag="IXF98_001092"
     CDS             23783..24586
                     /locus_tag="IXF98_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1555422.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24656..25396
                     /locus_tag="IXF98_001093"
     CDS             24656..25396
                     /locus_tag="IXF98_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1555423.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25407..26411
                     /locus_tag="IXF98_001094"
     CDS             25407..26411
                     /locus_tag="IXF98_001094"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1555424.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26456..28219)
                     /locus_tag="IXF98_001095"
     CDS             complement(26456..28219)
                     /locus_tag="IXF98_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555425.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8903 a         5001 c         5814 g         8531 t
ORIGIN      
        1 ttttttgcga acattcattt agcaattttt agaaacttct gccgccaccg ccaaaagtcc
       61 cgccaccgct agaatgggta gtacttccgc caccgccgct tccaggaggt gggcttttgg
      121 ggattcttct tgtcgtgaca aaagaatttg tcagacgatc ggttttttca gttaatttga
      181 tactggattt ttctcggaat ggatatttgt agatgcctga tttcaattga taacgcgaaa
      241 cagtcacaaa ataaaagaca agactaagga taagagccaa aacaacagca atgatgattt
      301 ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct acacgataat
      361 gtcctcctgg aactccgtct tccacatatg ttgctgattt agacaggtaa gcttttgcag
      421 cattgtaata ggcagcattt gtcatctgat cataaacatc atctaagatc gaatcgattc
      481 tgctatcagt caaataatcg atcatattac cagaagtcga aatgtagatt tcacgctgtc
      541 ccatatctaa tagcaatact gagccatttt gatcatttcc gactgcgtct cttaagtaat
      601 tatcagcaaa gtctcttggt tcttctgaat tagatgtagt cgttacgatc aagactcgtc
      661 ctttgatttt ttcattcagc ttttctgcct gttgttccaa agattggatt tgatctgttg
      721 tgaataaccc agcctcatca tttaccgtag ctgtatctgc atgagtagcc accggccagc
      781 caacacttac tagcaaaaga aagaataaaa aaaatttttt catcatatca agtaacctcc
      841 gatcagaaag aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc ccaatttttt
      901 ctggctgatc ggcaggacac cgctgacctt tcctgtttgt ccattcattg cgtagtagta
      961 gactttttta cttgagtcat tgcttcgata agtaacaagc cataccggca gtaatacgta
     1021 attatttact tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg ttgtatatcc
     1081 atttgcggta tcttttaaca gcttctctga atagtctttt aattcagatt gaatctgtga
     1141 tttgattgcc tcatattcga tatctctttt ttctgcttgg aacccggcta aatactggct
     1201 tttaaaatca attgctttat ctaacgggaa aggttgcacg ccttcgacca tcttttgttg
     1261 cgtatttttt gataaggcat tttttactag ttctttaaaa gaaagcttgc ctgctcttga
     1321 aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct atccgccata tgcggataac
     1381 tgtaccattc gcctgcatgg caccatctac ttcagcatct gtagcccaat aagggaaata
     1441 cacacctgtc aacttatcga tttgatcctt attgaaaaaa gctttgggaa caaaccattt
     1501 tttcttggtc caagcgagaa atctgttgat tgcttcttct ttctcgacag caaaaggaag
     1561 tactttattt ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg gattatggca
     1621 gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg atctctgcac cacagttggg
     1681 acaaagaaaa agatctatcg ctgcggtatt atctgcagat tcgtccgctg tatctgaaaa
     1741 accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa gctgttcttg
     1801 ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct ttgctttctt cgccagccat
     1861 acgcgcttct ttttgctttt gctcatattg gctgacttct tcttctgtat agatattcag
     1921 gcagtattcg caatgaaatt tttggtcttt gggatcaaaa gtcaacggac cgccgcaatt
     1981 aggacattta tgtgtaagaa catccatcag ctgcacacct ttctaaataa attaattaac
     2041 ggggattcct ttaaacggtt taccacagtt tggacagaat ttagggatgc cgttggaaag
     2101 atccacgatc tcattgcatt cactgcatcg catttgtagc ttaggctgaa cgttttctac
     2161 aggtttggca gctccgcagt tggaacagaa ttttccggaa ttttctgtcc cgcaaactgg
     2221 acatgtccaa gtatccgcat ttccttgtgc agtttgttga ttttgttttt ctgcttgttg
     2281 tttgatttgt tcttggttat tttgagctgc ttgagaaaga taagaaccat ttgcattcat
     2341 tcccatacct acacccataa agccggtcat tgctcctgct tcattttttc cggctgcttc
     2401 cataccacgt gcaatggaac cttgcacgta tccttcacgt acgctaggat cacctagcat
     2461 cgccccttcg ttacgcatat tgatcagttt cacagaatca tcggtgtaag aaatgctggc
     2521 tacagccaca gaaacaatct ccatcccacg taattcacgc caagaatcat ccaatgcagt
     2581 atccatgtat ttgcttaatt ccaaactctt agatggtaca taagaaatcc gttgaccatc
     2641 tgcagacatc tgattgattg ctgattgtag cgctgttagg aattcagcta aatactgctc
     2701 attgatatca ttgatttcca cttgtgtttt gtttttaggg attgcgtttg tataaaataa
     2761 aatcgggtca acgacatgaa tagaataagt gccatgtgcc cgtaagaata attctgcgtt
     2821 atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc cctttatttc
     2881 ttggagattg atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa atttgaatcg
     2941 gtcaaaagtt tcagcaattg cttctttcag tgttccattg aacatggaag gagctgcgtc
     3001 atttttaatg gtgtaataac cttcctcagc ggtataatcg atgatttttc caccatcgac
     3061 caaaagcatc atcatgtttg gatagacatg gacgactgtt ccgtctgtta aaacatcttc
     3121 tgttccttta cgattggagc cacgtttgtc atctttgcgg actttgacac cttttgtcat
     3181 gaccgttgta tcactcatat tgtctggttc gatgacttct agccattgat ctgccagacc
     3241 gccgccaacc atacttgttg ctgctttgat aagacccata aaaaattcct cctctgattc
     3301 agctagtgcc aaacaattat tgtttattta ttataccata ggaagatgtg aaacttctcg
     3361 gactagagac ggaattgaaa tggaaaattg cgtattgttt aaatacctga aaaagccgag
     3421 aaggatagta gtttttttac ctaatatcac gtataataag agatagtgaa ttttttaaaa
     3481 aagggggatc cttatggcat atcaagcact ttatcgagtc tggcgttcgc agcgtttcga
     3541 agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa aatgctattg tttcccacaa
     3601 aacctcacat gcctacttgt ttacaggacc cagaggaacc ggaaagacga gtgcagcaaa
     3661 gattttcgca aaagcaatca actgtccgaa cagtaaagac ggtgagccat gcaacgaatg
     3721 cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa tcgatgctgc
     3781 cagcaataac ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt atgcgcctac
     3841 aaaggcagcg tacaaagtgt acattattga tgaggtacat atgctttcaa caggagcttt
     3901 caatgccttg ttgaaaacat tggaagagcc aaaagaaagt gtcatattca tcttagcaac
     3961 gaccgaacct cacaaaatac cagctacgat catttcacgg actcagcgat ttgattttaa
     4021 acggatcaat acggcagata ttgtcgaaca tcttgaattt atcttgaaag aatcgactat
     4081 cagctatgaa ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg ggatgcggga
     4141 tgcattgagt attcttgatc aggcaatctc gtttagtgaa ggaacaatta ctttagatga
     4201 tgccatgcaa gtgacaggta gcttaactga cgaaatgatg gacagttact tgagtgcttg
     4261 cgtgaaaaaa gaagtaccag aagcattgga aacattgaac caaattctcg cagccggcaa
     4321 agaatcacgt cgctttctag aggatatgtt acaatactgc cgtgacttgc tgatgtatca
     4381 gcaagcacct aacctagttg aggaacaatc gagtacgatc agaagtacat ttacagaatt
     4441 ggcacaaact gtttcagcgg atcagctgtt ttcaatgata aagattttga gcgatactca
     4501 aaatgaaatt cgttttacca atagtgccgc tatttatttg gaagtcgcta cagtcaaact
     4561 tgcacagcta ccgtctgctt ctgttccttc agcagatgta gcatttgcag atgatcagat
     4621 agtaaaacag ctacaacaag agattaagga actaagaaac gaaatcaagg aactacgcag
     4681 taatacagga attgagaata caaaagtaca gccggtacag caaacaaatc ataaaaaaca
     4741 gacaagtacc tatcgtgtac cgacggaacg agtatataaa gttctgcggg aagcgacaag
     4801 acagcatctg aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc tatctgtcac
     4861 tcagcgagca atgcttaaag caagcgaacc agttgcagct ggacctacag gactagtgat
     4921 tgcgtttgat tatgagattg tttgtcaaag agcagcaaat gacgaagaat tgcagctttc
     4981 ggtacacaac aacttaagcc gaatgatcaa agattatgcg cctgatcctg tttatattac
     5041 aagagaaagc tggccagaac ttcgaagaaa ttatttgacg caggcacaag aagagggaac
     5101 agaaaactct tttgcttctg aagactcaga aggagaaatc gagttgcttc ctcaagaaca
     5161 agaagtacca gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct ccgaagctgt
     5221 tacagtggta gatgattaat aaaaaggagt agataattat gatgcgtgga atgggcaata
     5281 tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa gcacaagagc
     5341 tgctaaataa aacagagttt acaggtaaag ccaccaatga attggtcaaa gcggttttta
     5401 caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat cctgatgata
     5461 tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca caaattgatg
     5521 tggaaactga gaaaaaatta gggaaatacg caaaaggttt gccaggattt tagttttaaa
     5581 ctgaaggagt aatgaatatg caatatccag aaccaattgc gaaactgatc gacagctaca
     5641 tgaaacttcc aggaatcggt cagaaaacag cgacaagact cgccttttat acgatcgata
     5701 tgaaagatga agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa cgcgatttgc
     5761 atttttgtag tatctgcgga aacatcacag aagaagatcc ctgcgaaata tgtagaaatc
     5821 cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta atggcaatgg
     5881 aaaaaatgcg agaatatcac ggactttatc atgttctcca tggtgtgtta tcaccgatgg
     5941 aagggacagg tccagaagac atcaatattt cttctctgtt gcagcggctg catgatgcag
     6001 atgtaaaaga agtgattatc gcaacaaatg caacaacaga gggagaagca acagctatgt
     6061 atctctcacg tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca catggtctgt
     6121 ctgtaggctc tgatatcgag tatgcagatg aagttacttt attgaaagct gtcgaaggaa
     6181 gacgtgagtt ataaatttct aattaagtgt tttttcagat acaatataaa agcagcacaa
     6241 gagatggggg caacaatgtg aacggactat ttattacaat cgaggggcct gatggtgcag
     6301 gaaaaaccag catcttgaat gaactttacc ctcttttgaa agaagtagca aaaacagaaa
     6361 tcattcaaac aagagaacca ggaggcattc cgattgctga agagatccga gcagtgatcc
     6421 ttgatccaaa aaatgatcgg atggatgaac gtacggaagc actgctttat gcagcagcaa
     6481 gaagacaaca tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa atcgttttat
     6541 gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc atcggcgtta
     6601 ccgagattgc tcggttgaat gagtttgcta cagaaggtac tacgccggat tttacccttt
     6661 atcttgacgt agattcagac accggattaa gaagaatcaa gaaaaacaga caaaatcaaa
     6721 tcgaccgttt agattcggaa ggtttggaat tccaccagcg tgtacgtcat gcttacttaa
     6781 agctggcaga agagaatccg gaaagaatcc acaaagttga tgcaagaaaa agctttgaag
     6841 aagtgctgca aacaagttat catacaatta ttgaacagta tccgcaattt tttgaaaact
     6901 aggaaggtga acgtttatga aaattatttt agcgattatc caagacaaag acagcaaccg
     6961 tttatcaaat gaattgattg atgcaaatat ccgtgccaca aaactatcat cgacaggcgg
     7021 cttccttaag gctggaaaca gtacgtttat cgtgggtatt gaagatgacc gtgtagaaga
     7081 agcattggaa atcatcaaaa aaacgtgtga atcaagaaag caatttgtat ctacacctgt
     7141 tacactggat atttcgatgg atgggggcgt cccttatcca gtagaagtag aagtaggcgg
     7201 tgccacagtc ttcgtcttac ctgttgaagg attccatcaa ctttaggagg agctgagcta
     7261 tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt gtatcagcag cttcaacgga
     7321 gttttgagca tgggcgtatc gctcatgctt atctttttga aggggagaaa ggaacaggaa
     7381 aacatgaagt aggtatatgg cttgcacagc acctgttctg tacccaaatg aaagacaatc
     7441 tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat ccagatgttt
     7501 tggttattga accggaagga caaaccatca aagtcgatca gatcagacgt ttgcagacgg
     7561 aattcagtcg cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa gaagctgaaa
     7621 aaatgaatag cagtgcggca aacagtttgt tgaaattctt ggaagagccc cctggagatt
     7681 ttttagcggt gttagaaaca gatgcagtag gaagaattct ccctactatt caatctcgtt
     7741 gtcaagttct gcattttcaa gaactttcga aagaagcact gatccatcga ttgcaagaag
     7801 agcagattcc attggaaaaa gcaaaacttt tggctttctt aacaaacagt ttaggaaaag
     7861 cagttgaaat ctctcaagat gaatggttta atgatgctaa ggacttgatt tatcaatggt
     7921 tcgtttactt gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa aaattggtaa
     7981 aatcatttaa agaaaaaaca caacagttta caggattatc tgtcttgatg ttttattatc
     8041 aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa cagataaacc
     8101 agataattga acggatattg atcgcagagc agaaactacg cagcaatgtc agttttcagg
     8161 ctgttgcgga acagttcgtt ttgcatacga ttttttagct tgccgcaaag gatgatgatc
     8221 aaggaggatg gaaatggtag aagtagtagg tgtccgatat agagatgcag gtcatatcta
     8281 ttattttgca ccaggagact ctgaatatgt ttatagtgac aaggtattag tcgaatctca
     8341 gcaatcctta catattgcta ctgttgtcct tcctaaaaaa gagattgatc ccaatgacct
     8401 tcctgaagct atccgtccga tcttgcataa agcgtcacaa gctgaattag aaaaagacaa
     8461 aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag tgcagttgcg
     8521 tgagttggaa atgaaaatgg tcaaagccga atacacattt gatcgaaata aattgatttt
     8581 ttctttcaca gcggacgggc gaattgactt tagagaattg gtaaaagatc tggccgctgt
     8641 atttcgtact cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa agataatagg
     8701 cggaattggt ccatgtgggc gtccattatg ctgttcttct tttttaggcg atttcgtgcc
     8761 tgtatcaatc aaaatggcaa aagatcaaag tttatctttg aatccaacaa aaatttccgg
     8821 tttatgcgga cgcttgatgt gctgtctgca atatgagagc gaagaatacg agacagcgaa
     8881 aaaagaactc cctgactttg gaaaagtaat tgctacacct gatggtaaag ggaaagtcgt
     8941 agggttgaat ctgcttagcc gtatcgttag tattcggctg actggacggg aaaatcctgt
     9001 cgaatatgaa tgggatgaat tgaaagaatt tatccaagta ggtgagatga atggataaga
     9061 gatcattata cgacggattg aatcaattag aatcagagtt gagaaataca ttaacagaac
     9121 ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact ttggagatgg
     9181 aaaatcaacg tctgcgggaa cacctgcggg aattgaacca agttgcacaa gctccagctg
     9241 aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt tatgaagaag
     9301 gattccatgt ctgctacgat ttatatggat caagaaggga caatgacgag ccttgtgcgt
     9361 tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc aataacaaga ggagactgga
     9421 gcggagaaat actgctccag tctttttatt aaggagattg tgacagatgt ataatcaaaa
     9481 aagtttcaag ggaaatccgg tatatgggga tttatatctt gtaccgacgc caattggaaa
     9541 tttagaagat atgagctaca gaagtgttcg tatgctgcaa gaatccgatc ttatcgcaag
     9601 tgaggatacg cgtaatacac aaaagttatt gaatcatttt gaaatccata caccccaaaa
     9661 aagtttacat gaacataatt ataaagaacg tgtccctcaa ttgattgctt tgttaaaaga
     9721 aggaaaaacg attgctcaag tcagtgacgc aggaatgcct tccatcagcg atcctggaca
     9781 tgagctagtt ttagcctgca taaaagaagg gatttctgtg atctctatcc caggacctac
     9841 agctgggatg acggctttga ttgcttcagg gctagtgcct caaccatttt tattttatgg
     9901 cttccttcca cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa aagaacagac
     9961 agttacgctg attttttatg agtcacctta tcggatcact gctacattga cgaatttctt
    10021 agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg tccatgaaga
    10081 ataccttaga ggaagtacgg aagagctact cgcttatatc gaagaacatc cagtaaaggg
    10141 tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg aagttgatga
    10201 agagttggga acgttaaagg aacaagtaga agcaaagatc gcttcaggcg aaaaaccaaa
    10261 tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag tctaccgtca
    10321 gtaccatgaa ttagactgag atagtataag ttgtctataa tgaaagacta atgaggaaat
    10381 cagctactaa cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata atgaataaga
    10441 ggtgaggatg atggagctgc aatttccttt acataatgat acatcaagaa aaatcattca
    10501 tattgatatg gatgcttttt tcgcttcggt tgaagaacga gatcatcctg agtttcgcgg
    10561 acatccatta gtaattgcca gacaccccag tgacactggt gggcgtggag tagtgactac
    10621 agcaaattat gaagcaagaa aattcggaat tcattcagca atgagtgcac aaaaagcgta
    10681 tgagctttgt ccgcaagcca tctttaagcc tggcgaccat cataaataca gcgatatttc
    10741 caaacaagta cgggaagtat tctatcgtta tacagatatt gtggaaccag ttagtatcga
    10801 tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc aagtcagcca tcaaaatcgc
    10861 ccgaatgatc caagcagata tttgggagag tgtccagcta acatgttctg cgggagtaag
    10921 ttacaataag tttttggcca aattagcatc agactatcaa aaacctagag gaatcacggt
    10981 agttgcccct caagacgctg tgccattttt aaaagctttg ccaattgaaa aatttcatgg
    11041 ggtagggaag aaaactgtac ctagaatgca tgagctaggg atttatacag gaaaagacct
    11101 atatgaatgc acagaaatga tgttgattcg aaattttgga aaaatgggct attctcttta
    11161 tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt acaagagagc gaaaatctgt
    11221 gggaaaagag catacttatg ggcagccgtt gcaaacggaa gaagcagtgc tgactcagtt
    11281 gcgccaacta gctgaaaaag tagaagaagc actgagaaga gtccaaaaac atgggaaaac
    11341 agtagttttg aaggtcaggt acacggacta ttctactgtg accaaaagag tgacattgcc
    11401 agaatatatc tataaaaaag aagcactctt ttatcaagca agccttatat gggaggaaat
    11461 cttgggagta gaaaaaggaa tccgattgtt agggattact ttgacgaatc tggacccgat
    11521 gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa aacagctttg
    11581 agagttgaaa ccggaaatcc gttttctctc ccaaagctgt ttttatatca agtcttgctt
    11641 tttgatttgt tcataggttt ctgttgcatc cgtacatttt tcccaaatca caacttgatt
    11701 ggattctaaa gatttttgga cgtcaatgat ccgctggttg ctacttccgc gaaattgtag
    11761 ttttaggtca cgtttgctta attcaaagcg tccgtcaaca aggatatcga tttgcgaaag
    11821 gagttctaat ttatcttctg aatcttgtag caattcatca aaggtatagc ctgtccatga
    11881 ccagatatct tttgtcgtac cgaactcttt tcggatacgt cgtacgactt gcagacaaac
    11941 atctgtattc aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt aatcgttgcg
    12001 caagtcagcg atgatttgtt cttctaattc aggtgtgaat ggtttgccat atcggaaatt
    12061 ttgtgcagct ttgttgaagc agccttcgca ggcaaataag cagccactaa cgtaaagact
    12121 gttgcggaca ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa tatagttttg
    12181 gctgtagtct tttgctaacc attcttttgg ttttggattt ctcataaaaa aacttctttc
    12241 atttaaggtt caaagatata gctattgaag ataaaatcta ctcgaccaca taaataagaa
    12301 caaactgacc taaatccatt gttcaagaaa gcttgttctt attttgatag agggcgttca
    12361 aagatacaaa gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc tcttattctg
    12421 aataaacggt gttcaaaagc ttgcatctgc agtaataagc ccaactaaac gaaaaatatg
    12481 aggaactatt tttgtttagc tattcttatt acttgatgca ggacagcttt ttcacaacct
    12541 catttcatgt gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga
    12601 gcttgcggat ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca
    12661 tgatttccac attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa
    12721 ccacattgat agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat
    12781 gcaaaatccc atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag
    12841 tagtggatga atccacctga acaatatttt ggataatcct tctcaaattc caatttttca
    12901 aatggcgttg ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct
    12961 gtgatatttt cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga
    13021 ctttcacttg gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct
    13081 gcattagctt ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt
    13141 tcccattcgc caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat
    13201 acagtagctc gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc
    13261 ccgaaagcac catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt
    13321 tctacacgat agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag
    13381 aatttattct gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca
    13441 cctaaattca ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag
    13501 aatgaacgac agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa
    13561 ttcagaatat caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg
    13621 taattaggat cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg
    13681 aagatagctg tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt
    13741 tgaatttctc tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt
    13801 tgtccatttg aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag
    13861 atatcttttt tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat
    13921 tcttccgcgt cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca
    13981 tctgttcggt cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt
    14041 gagatttgtg ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt
    14101 ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta
    14161 taaggatgat aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata
    14221 tgtggtggca acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc
    14281 tgtgtattga aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg
    14341 ttgattagct tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt
    14401 ttggtacgat aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata
    14461 tgttccacga tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt
    14521 tcttggacaa ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag
    14581 ccgtgaattt tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca
    14641 acatggcggc catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg
    14701 acagtttttt cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc
    14761 tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg
    14821 tgctgtataa ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga
    14881 aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga
    14941 ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta
    15001 gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa
    15061 aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa
    15121 gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca
    15181 agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg
    15241 ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac
    15301 tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga taccctaaat
    15361 gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa
    15421 aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc
    15481 gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag
    15541 ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa
    15601 ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc
    15661 catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt
    15721 ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg
    15781 caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt
    15841 ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat
    15901 ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga
    15961 tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt
    16021 tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta
    16081 tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat
    16141 aattggattg ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt
    16201 ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat
    16261 tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata
    16321 ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc
    16381 agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact
    16441 acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat
    16501 cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg
    16561 tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag
    16621 taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca
    16681 tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa tttctttaat
    16741 gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc
    16801 cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga
    16861 ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta
    16921 tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata
    16981 tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca
    17041 tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag
    17101 tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg
    17161 tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa
    17221 agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa
    17281 aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc
    17341 ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga
    17401 ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta
    17461 ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt
    17521 cgggatgatc gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc
    17581 agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat
    17641 cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat
    17701 cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg
    17761 gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac
    17821 gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa
    17881 tagaactaaa ttttgaatac catttatttg gaataagagg ttgtgacacg atttttgtca
    17941 caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg
    18001 atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc
    18061 tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat
    18121 gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat
    18181 tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact
    18241 ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa
    18301 atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct
    18361 tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct
    18421 tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag
    18481 tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt
    18541 gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg
    18601 gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa
    18661 gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt
    18721 acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa
    18781 atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag
    18841 cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt
    18901 tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac
    18961 tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca
    19021 tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg
    19081 gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat
    19141 cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt
    19201 tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc gattggctac
    19261 gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa
    19321 acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa
    19381 aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca
    19441 ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa
    19501 ttacatggat accggattga actcggggat atcgaacatt atctgctcca agacaaccga
    19561 atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct
    19621 tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa
    19681 gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat
    19741 ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat
    19801 cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag
    19861 ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt
    19921 atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac
    19981 agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg
    20041 tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa
    20101 ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg
    20161 gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag
    20221 atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa
    20281 caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc
    20341 ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt
    20401 ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac
    20461 gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca
    20521 gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca
    20581 tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag
    20641 aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc
    20701 gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata
    20761 tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca
    20821 tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta
    20881 agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt
    20941 tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt
    21001 tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga
    21061 agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg
    21121 tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt
    21181 cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg
    21241 gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag
    21301 aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt
    21361 acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc
    21421 atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa
    21481 tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt
    21541 ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa
    21601 tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc
    21661 gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat
    21721 acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct
    21781 gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg
    21841 ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat
    21901 cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg
    21961 ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa
    22021 gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca
    22081 ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa
    22141 cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc
    22201 ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag
    22261 aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc
    22321 aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt
    22381 accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat
    22441 caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac
    22501 gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa
    22561 agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga
    22621 taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat
    22681 atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt
    22741 ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca
    22801 aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg
    22861 ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa
    22921 caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag
    22981 tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga
    23041 ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa
    23101 aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac
    23161 gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt
    23221 tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga
    23281 atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag
    23341 taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt
    23401 tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt
    23461 taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat
    23521 tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt
    23581 ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag
    23641 ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt
    23701 atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg
    23761 aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa
    23821 agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat
    23881 aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag
    23941 catgcagcag cacttattga gttttctagt cgataattta cttttattcg cattcaacta
    24001 tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt
    24061 attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc
    24121 agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt
    24181 gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga
    24241 agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact
    24301 catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc
    24361 tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg
    24421 tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt
    24481 gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga
    24541 acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt
    24601 attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa
    24661 attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta
    24721 tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa
    24781 attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca
    24841 tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa
    24901 tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt
    24961 gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg
    25021 tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat
    25081 cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag
    25141 tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg
    25201 aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc
    25261 taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa
    25321 aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa
    25381 agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa
    25441 gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt
    25501 tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag
    25561 aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag
    25621 gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg
    25681 gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat
    25741 tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat
    25801 tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt
    25861 tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat
    25921 caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag
    25981 aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg
    26041 tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca
    26101 atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt
    26161 tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct
    26221 cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct
    26281 ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag
    26341 tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct
    26401 tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata
    26461 caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt
    26521 cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac
    26581 gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg
    26641 cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt
    26701 ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga
    26761 tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga
    26821 aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg
    26881 tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct
    26941 tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca
    27001 tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct
    27061 cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt
    27121 tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa
    27181 tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt
    27241 aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac
    27301 ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac
    27361 tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa
    27421 gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat
    27481 agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata
    27541 aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg
    27601 atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa
    27661 gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa
    27721 cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg
    27781 gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg
    27841 agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg
    27901 agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta
    27961 gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt
    28021 tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag
    28081 tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt
    28141 ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa
    28201 ttcctgttaa aaataacaat gataatataa tgattatgct ccactttat
//
LOCUS       DACETN010000027        28091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DACETN010000027 DACETN010000000
VERSION     DACETN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.028"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(97..678)
                     /locus_tag="IXF98_001096"
     CDS             complement(97..678)
                     /locus_tag="IXF98_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1555426.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(861..1487)
                     /locus_tag="IXF98_001097"
     CDS             complement(861..1487)
                     /locus_tag="IXF98_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1555427.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1509..2840)
                     /locus_tag="IXF98_001098"
     CDS             complement(1509..2840)
                     /locus_tag="IXF98_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1555428.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2995..3396)
                     /locus_tag="IXF98_001099"
     CDS             complement(2995..3396)
                     /locus_tag="IXF98_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1555429.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3482..3688)
                     /locus_tag="IXF98_001100"
     CDS             complement(3482..3688)
                     /locus_tag="IXF98_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555430.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3783..5285)
                     /locus_tag="IXF98_001101"
     CDS             complement(3783..5285)
                     /locus_tag="IXF98_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1555431.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5303..5758)
                     /gene="argR"
                     /locus_tag="IXF98_001102"
     CDS             complement(5303..5758)
                     /gene="argR"
                     /locus_tag="IXF98_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1555432.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5856..6473)
                     /locus_tag="IXF98_001103"
     CDS             complement(5856..6473)
                     /locus_tag="IXF98_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555433.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6694..8067
                     /locus_tag="IXF98_001104"
     CDS             6694..8067
                     /locus_tag="IXF98_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1555434.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8142..9254
                     /locus_tag="IXF98_001105"
     CDS             8142..9254
                     /locus_tag="IXF98_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1555435.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9326..10963)
                     /locus_tag="IXF98_001106"
     CDS             complement(9326..10963)
                     /locus_tag="IXF98_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1555436.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11131..11739)
                     /locus_tag="IXF98_001107"
     CDS             complement(11131..11739)
                     /locus_tag="IXF98_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1555437.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12123..13274)
                     /gene="queG"
                     /locus_tag="IXF98_001108"
                     /pseudo
     CDS             complement(12123..13274)
                     /gene="queG"
                     /locus_tag="IXF98_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
     gene            complement(13293..14045)
                     /locus_tag="IXF98_001109"
     CDS             complement(13293..14045)
                     /locus_tag="IXF98_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555438.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14186..15136)
                     /locus_tag="IXF98_001110"
     CDS             complement(14186..15136)
                     /locus_tag="IXF98_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555439.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15137..15976)
                     /locus_tag="IXF98_001111"
     CDS             complement(15137..15976)
                     /locus_tag="IXF98_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1555440.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15973..16719)
                     /locus_tag="IXF98_001112"
     CDS             complement(15973..16719)
                     /locus_tag="IXF98_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555441.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16957..18327
                     /locus_tag="IXF98_001113"
     CDS             16957..18327
                     /locus_tag="IXF98_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1555442.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18382..19278)
                     /locus_tag="IXF98_001114"
     CDS             complement(18382..19278)
                     /locus_tag="IXF98_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1555443.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19559..20236)
                     /locus_tag="IXF98_001115"
     CDS             complement(19559..20236)
                     /locus_tag="IXF98_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1555444.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20327..21232)
                     /locus_tag="IXF98_001116"
     CDS             complement(20327..21232)
                     /locus_tag="IXF98_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1555445.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21407..22861
                     /locus_tag="IXF98_001117"
     CDS             21407..22861
                     /locus_tag="IXF98_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1555446.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22877..23908
                     /locus_tag="IXF98_001118"
     CDS             22877..23908
                     /locus_tag="IXF98_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1555447.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23915..25102
                     /locus_tag="IXF98_001119"
     CDS             23915..25102
                     /locus_tag="IXF98_001119"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1555448.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25258..26640
                     /locus_tag="IXF98_001120"
     CDS             25258..26640
                     /locus_tag="IXF98_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1555449.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26612..26998
                     /locus_tag="IXF98_001121"
     CDS             26612..26998
                     /locus_tag="IXF98_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555450.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27198..27866)
                     /locus_tag="IXF98_001122"
     CDS             complement(27198..27866)
                     /locus_tag="IXF98_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1555451.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7828 a         5744 c         5156 g         9363 t
ORIGIN      
        1 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
       61 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
      121 caatctttct ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
      181 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
      241 ttcatcggat agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
      301 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
      361 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
      421 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
      481 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
      541 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
      601 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
      661 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
      721 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
      781 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
      841 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
      901 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
      961 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
     1021 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
     1081 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
     1141 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
     1201 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
     1261 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
     1321 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
     1381 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
     1441 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
     1501 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
     1561 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
     1621 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
     1681 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
     1741 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
     1801 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
     1861 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
     1921 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
     1981 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
     2041 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
     2101 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
     2161 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
     2221 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
     2281 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
     2341 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
     2401 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
     2461 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
     2521 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
     2581 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
     2641 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
     2701 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
     2761 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
     2821 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
     2881 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
     2941 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
     3001 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
     3061 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa
     3121 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
     3181 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
     3241 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
     3301 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
     3361 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
     3421 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
     3481 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
     3541 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
     3601 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
     3661 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
     3721 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
     3781 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
     3841 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
     3901 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
     3961 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
     4021 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
     4081 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
     4141 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
     4201 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
     4261 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
     4321 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
     4381 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
     4441 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
     4501 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
     4561 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
     4621 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
     4681 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
     4741 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
     4801 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
     4861 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
     4921 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
     4981 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
     5041 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
     5101 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
     5161 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
     5221 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
     5281 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
     5341 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
     5401 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
     5461 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
     5521 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
     5581 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
     5641 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
     5701 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
     5761 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
     5821 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
     5881 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
     5941 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
     6001 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
     6061 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
     6121 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
     6181 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
     6241 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
     6301 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
     6361 ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc
     6421 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
     6481 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
     6541 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
     6601 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
     6661 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
     6721 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
     6781 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
     6841 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
     6901 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
     6961 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
     7021 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
     7081 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
     7141 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
     7201 tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat tcccgagatg
     7261 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
     7321 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
     7381 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
     7441 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
     7501 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
     7561 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
     7621 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
     7681 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
     7741 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
     7801 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
     7861 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
     7921 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
     7981 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
     8041 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
     8101 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
     8161 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
     8221 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
     8281 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
     8341 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
     8401 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
     8461 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
     8521 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
     8581 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
     8641 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
     8701 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
     8761 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
     8821 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
     8881 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
     8941 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
     9001 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
     9061 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
     9121 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
     9181 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
     9241 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
     9301 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
     9361 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
     9421 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
     9481 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
     9541 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
     9601 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
     9661 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
     9721 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
     9781 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
     9841 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
     9901 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
     9961 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    10021 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    10081 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    10141 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    10201 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    10261 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    10321 ctccaaagta acgtaatcct agcacaaccg ctgtattatg gagagcatca aacaaaatga
    10381 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    10441 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    10501 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    10561 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    10621 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    10681 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    10741 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    10801 tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa
    10861 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    10921 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    10981 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    11041 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    11101 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    11161 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    11221 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    11281 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    11341 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    11401 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    11461 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    11521 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    11581 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    11641 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    11701 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    11761 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    11821 aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata
    11881 tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg
    11941 tttttttatt tttgaatgaa aaacgcagat tctgtaacat tcactgcaat aatccataaa
    12001 aaaaagcaaa aaaatttttg atttttatca aattctctta aaaaaatgta aaaaatacaa
    12061 aaaaaccagt aattgttaca acaattactg gacaaaaact ttgaataatg ttaactattt
    12121 ttttatttta actttctccg caataatttc tgggcatgtt ccaattcgtt tataacgact
    12181 tcttctgttt ctttagctaa tatttcatct aatacatcaa gccattttta cggttctttt
    12241 gttcctagac gtccaatagc ccaagctgct gttcctctga tgacagggcg gacatctgtt
    12301 aaacattctg aaatctcagg caatgccgtt cgatcaccta gattagctaa agcaatcaat
    12361 gcgtttctct gcaaaggttt cttcccacgc catgaaccag ctaaatgccc aaattgctgt
    12421 ttgaagtctt tattagacat cgaaagcatt ggctttaatt ttggatatat ttcgtctact
    12481 tttggttcca tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg gcagactaac
    12541 tggcagatat cacatccata aatgacattt cgcatttttt tacgatattc ttccggcatc
    12601 atcccttttg tctgagtttg ataagacaaa catcgctggg cattcattct tccgtcacca
    12661 agcaatgctt ttgtcggaca agcagtcacg cagcgcatac agtcaccgca accaaattct
    12721 cctggtttat caggctcaaa tacaatatta gtaacgatct caccaagata gacaaatgaa
    12781 ccgaactctt cagtgatcaa caatccgttt cgtccaataa atcctaaacc agctcgctgt
    12841 gctacagcga catcgatcag ttctccagta tctacttgcg gagctagacg ccattcctct
    12901 tctttttgcc atttttctgc tcttgtttgg atgaattcga tcaatcggtt caaacggtct
    12961 ctgagtatat catggtaatc gatcccccaa gaagcacgag caaactgccc gcgtttttca
    13021 tccctaggca cttcttcatg gatcctagta ggatacgcaa gtgcaatgga aatgatcgat
    13081 ttcggctgat caaatgtcat ttctggataa agacgctcat cgatatttgg atgttcaaag
    13141 ccagaagtat ggccagctgc tttttgttct tccaacgatt cttttaatga atcaaaaggt
    13201 tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg tatgatttct
    13261 tcttttaatg tcatggtctt cctcgcttgc tgttaacggc tgtataaccc ataatatagt
    13321 ttttcttttt tcagctggac aaactggatc tgctgatcgg caatcttctt ttcttccatc
    13381 catttttttc ttgcagcaaa tacttcccaa taatttgttg aaattaattt tcttttgtta
    13441 tctaatgcat taaaaaaatt caatgaaact ggttttgtac ttaacagttc atggagcctt
    13501 acctcttccc atggcgaaaa ctcagacaga tcgcgggaat acaatacttc ttcatagaga
    13561 tttttttgct gtgtgtacgt gaatgtttgc gggattttcc caggcctagc aatttctact
    13621 agacgattat aaaacatatt cattaatttg ctgttggcaa taaattgact tttataatag
    13681 gccgactgga tctttttgat tactccttct gctttgatga ctgaaaataa tgcaggccat
    13741 gctttgacgg ttatttcgtc gttttgttta actaatacgc caggtgtgaa gccgactgga
    13801 gaaaggaaca caaaataacc gataccggta ttttcatcta aaggctgaaa ttcatcaaac
    13861 aaccatttgc ctatgtacag ctcttcatct ggttctgttg tctcattttg atttgctatg
    13921 aataatttag ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc
    13981 tgctggctga tcaatcgaaa gacttcttct ttaatcgtat atttatcttt acgtgtaaaa
    14041 ttcatagcca caccttcctt ccttactcta actataacgg aatttcttca aaaaaggtat
    14101 ctgtacgacg aaaaacagca taattttttt tcatatctct tataaaaaat gcaaggtctg
    14161 ctcctcaagc tagaccttgc acttttcagc tttcttttgt ttgtgacaaa ccttcatgga
    14221 tcgtttcaat attccatttc atcatctgat agtaagaatc tccttgttct ccttcttttg
    14281 ctatggaatc agtgaacagt ttatcataga ttttcaaacc cgtttctttt gctacccgtt
    14341 ccatgcttct tgagtctaca ctggtttcca cgaataaaac cggtacttct tttgctctta
    14401 tctgatcaat gatcgctttc atttgatcag gcgtaccttg actttctgta ttgatctccc
    14461 atatataagc tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg
    14521 ttactaataa ttttttgttc tcttcgatag aagcaaaact ttccttcgct tgtgtatcta
    14581 attcttttag tttttccaca tatgcttctg catttttttt atagagtgtc gcattttctg
    14641 catctttttc agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt
    14701 ctagccaagc atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt
    14761 ctacattttt gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca
    14821 agttatcgaa ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatctg
    14881 catcgcttgc ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga
    14941 ctggtacgat actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca
    15001 agatcgaatt ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag
    15061 aagcctgctt tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg
    15121 atttttttgt tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa
    15181 ataaaaaagc aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc
    15241 aattgtaact ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag
    15301 ctgtaatgat cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga
    15361 tcaacatggc aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg
    15421 tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca
    15481 ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga
    15541 cgattccact aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc
    15601 caaataaaat atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta
    15661 atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct
    15721 gagtcacgaa accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt
    15781 aactaaaacc aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt
    15841 ctcccatcaa agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc
    15901 ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa
    15961 taaaggattg gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata
    16021 ggccaattga aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg
    16081 tttatttaaa agaataacct catcgaaata ttcttctact ttatgaagat catgatggac
    16141 gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc
    16201 acttaacgca tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc
    16261 tagagctctt gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct
    16321 tttggcatat tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt
    16381 tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa
    16441 tacatcaatt ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt
    16501 acggactgag tttaccgact gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa
    16561 ctctagcaaa gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt
    16621 aattttttgt ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc
    16681 ggctgaaagg tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt
    16741 ttacctaact ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat
    16801 gatgaaaaag tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca
    16861 tcaagagttc aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt
    16921 ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga
    16981 agtcttagtg cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg
    17041 tttatgggtg cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg
    17101 attgcaggat tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat
    17161 ccttcgactg gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac
    17221 ttgaccgctt ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct
    17281 gtaggatctt atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt
    17341 gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa
    17401 ttttggtttt cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg
    17461 gtaatcttat ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa
    17521 aatggtggat tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt
    17581 gcttcctatc aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa
    17641 aagacgatca ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt
    17701 gctattttta tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct
    17761 tttgtacaaa ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc
    17821 gtgattacag cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc
    17881 tatacacttg ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt
    17941 gcgccttttt atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat
    18001 tatcttctac caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca
    18061 agtattttgc ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa
    18121 aagaagcacg cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg
    18181 aattatgtga cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa
    18241 acgagaatct cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt
    18301 gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt
    18361 cgttcttttt tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata
    18421 attctccatg ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc
    18481 ggctatttga tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc
    18541 gctgttggta ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc
    18601 agaagcttct accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt
    18661 tgactctgct tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg
    18721 attcgtataa ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt
    18781 ttctgctgcc tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag
    18841 ctcacgtgct ttttggttag caactgctga taaaccagct attccctctt gatttccctg
    18901 aatttctgtc tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata
    18961 atcatttacg attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt
    19021 ttgtgtctct tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt
    19081 cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag
    19141 acccgcaact cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat
    19201 ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca
    19261 ctcattttca tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac
    19321 gcttcatatt cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt
    19381 ataagaaaat ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc
    19441 tgcattgttc gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg
    19501 tcttgatatt ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc
    19561 ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg
    19621 cctcagtcag tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct
    19681 gattttcctg tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt
    19741 ccaatgagag atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat
    19801 acataaattg tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc
    19861 gctgatacgg cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt
    19921 ctttttccgc cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg
    19981 ctgctcgttc caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt
    20041 aaaacggaaa ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta
    20101 tatctatttt tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag
    20161 cagaagcttc tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga
    20221 tgataagtgt acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac
    20281 cgccacaagt ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag
    20341 aaactgcggt ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat
    20401 ttttgttttt catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca
    20461 ttcggctaaa tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt
    20521 catccgtcag agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct
    20581 tgatactggt acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta
    20641 aatgctgggc gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga
    20701 ctgtttcaac agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat
    20761 cttttaaaag gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca
    20821 aggtttcttc cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag
    20881 aagagaccat cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag
    20941 cactcccacc aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa
    21001 acgtcgagtc tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat
    21061 ttttcacatg ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa
    21121 tagcaggacc aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca
    21181 attctttttc tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc
    21241 ctccttactc ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta
    21301 aaaaggaagc gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa
    21361 ccggtttact tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat
    21421 gatccaacgt gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt
    21481 aagcacttat tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac
    21541 gaatgcaaaa atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat
    21601 tatttttgat cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt
    21661 caaaccatgg ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac
    21721 caatttcttt ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt
    21781 tttcatctta ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc
    21841 agctttgagt acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc
    21901 ttctctaggt gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta
    21961 tcttgttact cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc
    22021 tgctgttatt tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac
    22081 tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca
    22141 aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc
    22201 aacaactgcc gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg
    22261 gatcgttggt ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct
    22321 tacacgcgtc atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc
    22381 ttactttttc tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt
    22441 gttctacttc cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt
    22501 tgaatatggt caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc
    22561 tttactagat aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc
    22621 ttctgggatg actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt
    22681 caaagcttat gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc
    22741 ttggaagatt acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa
    22801 attggctcca gctgatacaa tggaagatga acttcacgat gcagtagttc cttcccatta
    22861 agaaaggatc ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat
    22921 aaccttggag aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt
    22981 cagtttaaaa tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat
    23041 cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg
    23101 ccacttttct ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa
    23161 tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg
    23221 cgaaaatgaa gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc
    23281 aggtacaatg gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac
    23341 ctctcgcggc accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa
    23401 cttgacggga gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc
    23461 ttatgctcct ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag
    23521 tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca
    23581 aaaaaatctt gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa
    23641 agaagccgct cgcttgacta gccccgataa aagaatccaa gtagatattg ctacagatgc
    23701 ttctagtgtg gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg
    23761 aaaactggca catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg
    23821 cttctcagca tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtgacaga
    23881 attcaaaatc aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac
    23941 aaaatttgcc gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat
    24001 caatttgatt ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac
    24061 ttatggaacg caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac
    24121 aaactttgac gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa
    24181 agcaaactgg gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc
    24241 aatcgatcat gggtttgagt tattagtgga aggaacaatc cctaatggtg caggactttc
    24301 cagtagcgct tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa
    24361 aacagatcgc ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt
    24421 caattcaggt atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt
    24481 attagataca aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat
    24541 cgtcatcatg aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg
    24601 tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg
    24661 agacttagat gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat
    24721 acgcagagct cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt
    24781 agaagctggg aacttagaag catttggtca actgttaaat gattctcacc actctcttcg
    24841 ctatgattat gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca
    24901 agaaggtgtc ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct
    24961 tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt
    25021 tgtcggctat gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga
    25081 tccagaagct gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaatagata
    25141 aaaataacct ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt
    25201 tttttattga cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg
    25261 tgggcgaatc aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt
    25321 attgactgca aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt
    25381 acaactaaat tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga
    25441 ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt
    25501 gccagagatc ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc
    25561 tattatatgg aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg
    25621 aaccatcatg tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc
    25681 tttggtaaaa caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag
    25741 accggaatct acacgactat aggaattggc gataacccgc tacttgctaa actagcctta
    25801 gacaatggct caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca
    25861 gaaaagttat ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca
    25921 aaacgtctga aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat
    25981 cacttgaaaa gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc
    26041 gatcgaagtt ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa
    26101 atcctagata gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc
    26161 gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg
    26221 gtaggatact ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg
    26281 aaaatcgaag cgacaaatgc tagcagagat ctagcagatg ctttattgac cattttccac
    26341 cagcattacc actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac
    26401 gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa
    26461 gttgattttg ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg
    26521 catagtctct tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca
    26581 ggtggtatga gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga
    26641 ctataacgag tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga
    26701 agaactagtc aacggtatca aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa
    26761 acaacaaatg acattgcaag aaatcgatag tatactgaca gaagcctttt tatctcgtaa
    26821 agcagtaatc attcagctca atttaaaaga tgaatttgga agactcttgg atgaggtcga
    26881 agggcacttc attggtgaag cctatgaaga ttactttatg attgaccatc agccgatatt
    26941 gtgggaagac gtacggtata ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga
    27001 ggttgtgaca gaagtgatca acttcaaaaa ataagaaagg atttttcgaa aattgcttct
    27061 taaatttttg tggaattttg gcttattttt cgaagaagca acttctggaa caacgtatat
    27121 tagatatcag aggttgtgaa aaagtcgctt agttttgagt aacaagccaa ggcgctgatt
    27181 tcacaacctc tgtctatcta tccatctatt cggtaagatt catgcatgac gtcttcttta
    27241 tttagtatgt agagcccttt ttcttctgac cattcaacat aaatcagctg ttttacatct
    27301 gcatagcctt ctttcttcaa gttctccata agccatgctt cagtcaattg attggactga
    27361 agcactttat gctgaatttg cccttcatct acaagcagga tcgataaagc tttgtcttca
    27421 tctgattttt tcaatacact catttgaccg gcattctcta ggataacatg tttggcattt
    27481 tcaagaaaaa agcattcttg ctgtcttaag attgctcgca actgctccat ctcgatatgg
    27541 ttatttgtca actctgtcat gttgatgacg ccgtctttga ttagaacaga tggctcgcct
    27601 ttgacccaac gattgacctt ttcatttttt tggacgatac gttcgatacc ataaatcatt
    27661 actgcccaaa gcgcaatggc aaataggaca tgccctacat ggacattgtc atcataggtt
    27721 gattcttcta agatcccacc taaaacaagc gtatagacta aatcaaacgg tgtgatttct
    27781 gacatggatt tcttcccaag caaacgtacc actaataata aaccgatcaa cccgaacacc
    27841 agtttcattg taattgaaaa aatcattttt tctcccccta actatctgtt aattaaatta
    27901 tatatgcttt ctcacgaaca cgcatggaat atacaaggaa gaaatgtatc cttttactaa
    27961 aaaaaggact gtgacactgt agtgaccccc aaaagttaga cttttttgga gtggagaaat
    28021 ccactccttt tttttatgct ctaaaatgat atttttacac aaccgttacg gttgttttac
    28081 gaaattggac g
//
LOCUS       DACETN010000028        28042 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DACETN010000028 DACETN010000000
VERSION     DACETN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.029"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(377..3655)
                     /locus_tag="IXF98_001123"
     CDS             complement(377..3655)
                     /locus_tag="IXF98_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1555452.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3926..4471)
                     /locus_tag="IXF98_001124"
     CDS             complement(3926..4471)
                     /locus_tag="IXF98_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1555453.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4483..5175)
                     /locus_tag="IXF98_001125"
     CDS             complement(4483..5175)
                     /locus_tag="IXF98_001125"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1555454.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5194..5478)
                     /locus_tag="IXF98_001126"
     CDS             complement(5194..5478)
                     /locus_tag="IXF98_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555455.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5491..6054)
                     /locus_tag="IXF98_001127"
     CDS             complement(5491..6054)
                     /locus_tag="IXF98_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1555456.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6282..6944
                     /locus_tag="IXF98_001128"
     CDS             6282..6944
                     /locus_tag="IXF98_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1555457.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            7068..8264
                     /locus_tag="IXF98_001129"
     CDS             7068..8264
                     /locus_tag="IXF98_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1555458.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8365..9741)
                     /locus_tag="IXF98_001130"
     CDS             complement(8365..9741)
                     /locus_tag="IXF98_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1555459.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9778..10644)
                     /locus_tag="IXF98_001131"
     CDS             complement(9778..10644)
                     /locus_tag="IXF98_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1555460.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10733..11758)
                     /locus_tag="IXF98_001132"
     CDS             complement(10733..11758)
                     /locus_tag="IXF98_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1555461.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11776..12450)
                     /locus_tag="IXF98_001133"
     CDS             complement(11776..12450)
                     /locus_tag="IXF98_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555462.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12634..13527)
                     /locus_tag="IXF98_001134"
     CDS             complement(12634..13527)
                     /locus_tag="IXF98_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1555463.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13601..15298)
                     /locus_tag="IXF98_001135"
     CDS             complement(13601..15298)
                     /locus_tag="IXF98_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1555464.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15291..16604)
                     /locus_tag="IXF98_001136"
     CDS             complement(15291..16604)
                     /locus_tag="IXF98_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1555465.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16604..20122)
                     /locus_tag="IXF98_001137"
     CDS             complement(16604..20122)
                     /locus_tag="IXF98_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1555466.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20415..20873)
                     /locus_tag="IXF98_001138"
     CDS             complement(20415..20873)
                     /locus_tag="IXF98_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1555467.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21171..21689)
                     /locus_tag="IXF98_001139"
     CDS             complement(21171..21689)
                     /locus_tag="IXF98_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1555468.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21691..22308)
                     /locus_tag="IXF98_001140"
     CDS             complement(21691..22308)
                     /locus_tag="IXF98_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1555469.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22305..23159)
                     /locus_tag="IXF98_001141"
     CDS             complement(22305..23159)
                     /locus_tag="IXF98_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555470.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(23156..24067)
                     /locus_tag="IXF98_001142"
     CDS             complement(23156..24067)
                     /locus_tag="IXF98_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555471.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24108..25442)
                     /locus_tag="IXF98_001143"
     CDS             complement(24108..25442)
                     /locus_tag="IXF98_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555472.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25650..26480
                     /locus_tag="IXF98_001144"
     CDS             25650..26480
                     /locus_tag="IXF98_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1555473.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26724..26973)
                     /locus_tag="IXF98_001145"
                     /pseudo
     CDS             complement(26724..26973)
                     /locus_tag="IXF98_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27164..27358)
                     /locus_tag="IXF98_001146"
     CDS             complement(27164..27358)
                     /locus_tag="IXF98_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555474.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         8156 a         6072 c         4861 g         8953 t
ORIGIN      
        1 attttgtgaa aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg
       61 cttttgaaca ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga
      121 aaaaaacgga aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag
      181 ctgattgaac accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag
      241 aacagccaat caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga
      301 catctattgg tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata
      361 aaaggagaaa ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta
      421 acaaaattcc tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc
      481 cttgagtgcc atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat
      541 ttggttttcc atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg
      601 ggggcacatc ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat
      661 gagcagacca tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt
      721 ctccttttga aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg
      781 tttcaacgat ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt
      841 gatttggtgt gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt
      901 ctgcattctc cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg
      961 gttttgtctg atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg
     1021 tttctccgtc aaaaatagca cctacagata atggaagacc atccactctt tccttttctg
     1081 tttgacgatt ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat
     1141 agcgcgtaac aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt
     1201 ttgatatggt atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt
     1261 tcaacgagtg gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct
     1321 caagattctc tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg
     1381 aagaggtata aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga
     1441 taatatctgt tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat
     1501 tgatcaattc atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac
     1561 cttctccctc aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta
     1621 cttttccgat cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat
     1681 gatacgcgat acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc
     1741 catcggcatc ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt
     1801 tatgcgcggg ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat
     1861 ttccattccg ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc
     1921 ctttttgcat agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga
     1981 acagtgtcat cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat
     2041 ttttctctgc aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg
     2101 tcaatcctct ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg
     2161 cgtatctcaa tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg
     2221 acccttctgg tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa
     2281 gaatctcttt tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat
     2341 ctggcgtaaa actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa
     2401 attcccgatt cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt
     2461 tatttttaat tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt
     2521 ttcctgaatg attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt
     2581 cttgcccagg aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga
     2641 tcgtcatatt ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata
     2701 atttttccca gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat
     2761 aatatgcctc taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt
     2821 tcgtggccgg aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt
     2881 tttccaagaa ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag
     2941 ctggcgcagc ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa
     3001 gtcgcatcgt atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga
     3061 catattcgat ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca
     3121 aatgattttt tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat
     3181 aggtccaaga ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg
     3241 cttgataata ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg
     3301 tggaagagag ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc
     3361 tttctccgaa aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct
     3421 tttcattttc tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac
     3481 tagttcttgg tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt
     3541 cgttcacaga ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac
     3601 tgagcataag gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta
     3661 ttcctcccgt aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg
     3721 tcaataaaaa acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt
     3781 tgttgataat aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata
     3841 aaaaagagca taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt
     3901 tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat
     3961 gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat
     4021 ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac
     4081 atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg
     4141 ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat
     4201 agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat
     4261 tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa
     4321 tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt
     4381 tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat
     4441 atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac
     4501 aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct
     4561 ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt
     4621 gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg
     4681 gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac
     4741 gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg
     4801 caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc
     4861 aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac
     4921 ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta
     4981 ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac
     5041 gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca
     5101 tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat
     5161 aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac
     5221 cacacataac attaggataa caataataag agatttcatc aaaaaagagt tccatttggt
     5281 cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt
     5341 acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg
     5401 tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat
     5461 aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta
     5521 ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc
     5581 ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt
     5641 ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg
     5701 tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc
     5761 taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc
     5821 tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg
     5881 atcttgttcg tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt
     5941 cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct
     6001 atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc
     6061 tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact
     6121 ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt
     6181 agtccgatac tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa
     6241 agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc
     6301 cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg
     6361 ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca
     6421 ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg
     6481 aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa
     6541 aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg
     6601 atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac
     6661 caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt
     6721 tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa
     6781 aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc
     6841 aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga
     6901 gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa
     6961 agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag
     7021 acattcatgt aagaatcgag aacagattca acaaggagga attttttatg gatcataaac
     7081 cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc
     7141 cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc
     7201 aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag
     7261 ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat
     7321 cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac
     7381 aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca
     7441 atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca
     7501 aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga
     7561 tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac
     7621 agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag
     7681 aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag
     7741 aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc
     7801 tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac
     7861 aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg
     7921 ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa
     7981 aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa
     8041 attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg
     8101 tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat
     8161 tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca
     8221 tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt
     8281 tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct
     8341 ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata
     8401 gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt
     8461 ttgcagacga agcatcaata caatacaagt caagatggag acgattggca atagcggaac
     8521 gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa
     8581 gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac
     8641 attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt
     8701 tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt
     8761 gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag
     8821 cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat
     8881 tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat
     8941 cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg
     9001 cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa
     9061 gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa
     9121 tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat
     9181 accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga
     9241 cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt
     9301 ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa
     9361 accttgcata tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc
     9421 gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc
     9481 atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa
     9541 aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt
     9601 ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat
     9661 gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga gattgatctc
     9721 tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta
     9781 aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat
     9841 aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt
     9901 agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac
     9961 tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca
    10021 cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc
    10081 gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt
    10141 atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag
    10201 cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc
    10261 gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc
    10321 atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg
    10381 tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact
    10441 ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac
    10501 aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg
    10561 attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg
    10621 gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt
    10681 atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt
    10741 tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag
    10801 ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc
    10861 ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa
    10921 agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga
    10981 ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga
    11041 tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag
    11101 tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac
    11161 tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg
    11221 agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata
    11281 attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta
    11341 gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt
    11401 tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct
    11461 gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt
    11521 gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt
    11581 aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg
    11641 tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa
    11701 aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct
    11761 taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc
    11821 ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt
    11881 gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat
    11941 ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat
    12001 gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct
    12061 catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt
    12121 tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat
    12181 agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga
    12241 tacttcacgt attttttgac accaataata gccatccaaa acgggaagat taatatctag
    12301 taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt
    12361 tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc
    12421 ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac
    12481 tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc
    12541 aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt
    12601 aaatttatta tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt
    12661 ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc
    12721 tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga
    12781 gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt
    12841 gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt
    12901 tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata
    12961 cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt
    13021 ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa
    13081 agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa
    13141 taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta
    13201 atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg
    13261 tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc
    13321 taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat
    13381 ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc
    13441 agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg
    13501 aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc
    13561 aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt
    13621 tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg
    13681 ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt
    13741 ctggccctgg attttctact cctacgatct gaatttcttc attatagctt ccataagaga
    13801 tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg
    13861 tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat
    13921 ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt
    13981 cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat
    14041 tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa
    14101 actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt
    14161 catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt
    14221 ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc
    14281 tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc
    14341 cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc
    14401 gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt
    14461 aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg
    14521 ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt
    14581 gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct
    14641 gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt
    14701 ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca
    14761 taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa
    14821 gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg
    14881 cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc
    14941 aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt
    15001 ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa
    15061 acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc
    15121 tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt
    15181 cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta
    15241 ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt
    15301 ttttctcccc gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt
    15361 aaagaaatca acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga
    15421 cctggattgc ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg
    15481 ttataaacac gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat
    15541 tcagccatct cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa
    15601 aattcaggga tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca
    15661 ccttttaatt cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca
    15721 aggccctctt tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct
    15781 gctggtttga tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc
    15841 cccccttcta tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca
    15901 gttgttcctc tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt
    15961 ttaggataga tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc
    16021 aaaatagcag caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga
    16081 tcaatcgatt cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct
    16141 ttgatacgtg caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa
    16201 gtccctattt ggacaagcct ttcttctgtg attagctgac gaatattgtt tctgttcgtt
    16261 tcagcgattt gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga
    16321 taataatcgg tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa
    16381 atgatatctg ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat
    16441 tcaaattgat cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg
    16501 acatcttccc ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt
    16561 agtaaaacaa gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct
    16621 ttttagattg tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag
    16681 caagcaggat atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat
    16741 cgttcccgac actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact
    16801 cttgctgtag atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta
    16861 gtgtacccgc ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat
    16921 aactaatttc taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt
    16981 ccttgatccg atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg
    17041 gatctggtgc caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa
    17101 ttgaaaattc tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca
    17161 aatcgattgg cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat
    17221 agccttccgg caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac
    17281 cttcttcatt cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga
    17341 cagattccga gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat
    17401 cgacatactt ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt
    17461 tcgattcttc tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat
    17521 cccccacttt cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt
    17581 ttctaactcg gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac
    17641 gatcctcttc atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga
    17701 gttggatagt ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag
    17761 gttctttgaa accttctggg ttctggctct caatgatttt gtattctgca tagttggtag
    17821 tttcttcact atccttataa acaaatgaat ctggctctcc gattggatga ataactttct
    17881 caccatccac gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt
    17941 tctcttcctc tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca
    18001 gtccgttacc atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc
    18061 cttcaattag tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt
    18121 tgacatacaa ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct
    18181 tgtgatattc tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg
    18241 aaccagatgt atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg
    18301 catatttttt gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag
    18361 cgatctctct gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca
    18421 tcttcgtttc aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg
    18481 cttccccact ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc
    18541 cattttgacc cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag
    18601 aagatgggaa atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa
    18661 tataggccat attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta
    18721 cttggtactt gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag
    18781 cttggttttc ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc
    18841 catgacgttc ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat
    18901 ctagatggta aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac
    18961 cagtttcatc ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct
    19021 ccaaggctgt aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat
    19081 cttcttctaa atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc
    19141 tcccgctaac gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt
    19201 gatacttgtt tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt
    19261 gcgaagtagg actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg
    19321 ctttattatc tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg
    19381 gacggttaaa gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc
    19441 cagaaaactc ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca
    19501 tcacagtcca gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg
    19561 cataactgaa ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat
    19621 tttctccgcc attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg
    19681 gagttcctcc gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga
    19741 tatcatttgt caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac
    19801 tattatggta aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat
    19861 ctttactcat ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc
    19921 cagcggttaa ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac
    19981 tgatttcctt tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat
    20041 cttcaggatg tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga
    20101 tgtacgcccc gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct
    20161 gaacggtttg agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag
    20221 catagtcgta atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt
    20281 tctctccgtc atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat
    20341 ttcctaacac ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc
    20401 acttgaagag gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat
    20461 gtgccggttt ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc
    20521 gtctcgactt ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg
    20581 ttttttacgg taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca
    20641 ttggcacttt cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata
    20701 tacttccctt caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct
    20761 gttgactcag cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca
    20821 aatagcaaac tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta
    20881 tactcctttc aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa
    20941 ttataattta atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac
    21001 caattataca acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa
    21061 caaaacttaa gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa
    21121 taaagcacaa atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg
    21181 gcagcgcata ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga
    21241 taatgtaaaa gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat
    21301 attcctgcaa ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta
    21361 tgtcctgaag gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt
    21421 ttttcataag gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa
    21481 tagatcaaac tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact
    21541 aaaacagcag caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc
    21601 aaaaaattga tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta
    21661 tcatcgattt tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg
    21721 cgtacgatgg ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc
    21781 aaaatagatt ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata
    21841 gaaaagcgcg cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc
    21901 tttttgttcc agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa
    21961 aaacaacgta gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg
    22021 atagtcgatc aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag
    22081 agagaagttc tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt
    22141 tttgtaatga taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg
    22201 caagacgcac aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat
    22261 ttctaagaaa taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct
    22321 cctgtcaaat ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc
    22381 gggatcaaga caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc
    22441 tgaaaaactt gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga
    22501 ggacctaaga aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca
    22561 ggtttcgtca atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt
    22621 tcagcagctt cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata
    22681 ttgaacgcat tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg
    22741 atcttggaga ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta
    22801 gaaagaacca aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa
    22861 gcaataaaag aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta
    22921 tttcttgcat agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg
    22981 attttttctg ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa
    23041 gtaaacacca tccaaagcaa tagaatgatc aaaatgattc ctattactgt catgaggaca
    23101 taacttagta ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt
    23161 tgccgtcacc tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca
    23221 gaaccaacac gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag
    23281 ctacgaacgc cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc
    23341 ctgttccatt tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag
    23401 aggtgatcac atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact
    23461 ttccccaaac gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt
    23521 gtaatgtagc taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca
    23581 acgcaggttt cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc
    23641 ccagaaatgt gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga
    23701 ctgctactcc tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt
    23761 tgattttttg attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat
    23821 tgaagatgac gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga
    23881 acattctttt gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg
    23941 tgaaactacc aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa
    24001 taaaaggcga gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac
    24061 gcttcaaatt ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc
    24121 ttgactaagt tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt
    24181 cctaaaagaa tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa
    24241 ggaactggtg tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt
    24301 cctttatccg ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg
    24361 atatcaaaac ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg
    24421 aattcataag aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca
    24481 ccccacgacc aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca
    24541 aaatcatccg gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg
    24601 gctactaatc cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta
    24661 ttgatcgtat ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact
    24721 ttatttttag tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct
    24781 gcgtttagcg cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta
    24841 aattcttttc cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt
    24901 gtcggtagat cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg
    24961 acttgtgtat ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc
    25021 caaagctgcg gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt
    25081 gcttgctgtg cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt
    25141 gcatttccag ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac
    25201 ttataattta catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata
    25261 gcttcttttc gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca
    25321 cttgtttttt cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg
    25381 catcctccta ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc
    25441 atattttacc tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt
    25501 gttctgaaaa taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac
    25561 tttatataat tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat
    25621 gtattaaaaa tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg
    25681 aagcaaaacc agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg
    25741 gccaatatgg cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag
    25801 gagaagaaaa atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg
    25861 aaatgttggc gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag
    25921 cagcgatgca aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg
    25981 cgatcgtagc tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg
    26041 cctatatcat tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat
    26101 cacaaggaag acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag
    26161 atatgctgtc tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag
    26221 tgaaagaatt agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca
    26281 aagaacgaaa agacattgct tatgacttct ataacttctc agagggagac caaagtagtg
    26341 aaggttcttg ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag
    26401 aacctaagat cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga
    26461 cacctccatt caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc
    26521 tttgtgagca aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc
    26581 tctagagcta taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta
    26641 tgttcaagcc atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact
    26701 ccttagccct taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca
    26761 tgtttttctt gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc
    26821 acatagtatc cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa
    26881 tctcttgatg atctcctgaa tattggctcg atattgatta taaattattt ttcttctata
    26941 ctttggtgtg aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc
    27001 ctttattgct ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat
    27061 taggtctaaa gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt
    27121 cagaaaaaaa tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt
    27181 tataaatgga aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta
    27241 tgaaatcttt atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat
    27301 ttatcagaag tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag
    27361 actctccttt cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa
    27421 aggggcgcag aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt
    27481 ttaataacta taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc
    27541 tccaattttt aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact
    27601 tatatatcga acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac
    27661 ttgcatctaa ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt
    27721 aatttttaaa ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc
    27781 tgtctcatcc agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg
    27841 aatagttgag ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata
    27901 caatgaaaaa caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact
    27961 cattactgta tgattttagc agcccaacca acatataccc tataaaagct aataagatgg
    28021 ttaggaatcc tttagaattt tt
//
LOCUS       DACETN010000029        27945 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DACETN010000029 DACETN010000000
VERSION     DACETN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.030"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            291..2030
                     /gene="yfcC"
                     /locus_tag="IXF98_001147"
     CDS             291..2030
                     /gene="yfcC"
                     /locus_tag="IXF98_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1555475.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2187..3371
                     /gene="iadA"
                     /locus_tag="IXF98_001148"
     CDS             2187..3371
                     /gene="iadA"
                     /locus_tag="IXF98_001148"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1555476.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3482..3685
                     /locus_tag="IXF98_001149"
     CDS             3482..3685
                     /locus_tag="IXF98_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1555477.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3786..4448)
                     /locus_tag="IXF98_001150"
     CDS             complement(3786..4448)
                     /locus_tag="IXF98_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555478.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4461..5963)
                     /locus_tag="IXF98_001151"
     CDS             complement(4461..5963)
                     /locus_tag="IXF98_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555479.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6511..8190
                     /locus_tag="IXF98_001152"
     CDS             6511..8190
                     /locus_tag="IXF98_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1555480.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8317..8795
                     /locus_tag="IXF98_001153"
                     /pseudo
     CDS             8317..8795
                     /locus_tag="IXF98_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
     gene            9014..9694
                     /locus_tag="IXF98_001154"
     CDS             9014..9694
                     /locus_tag="IXF98_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1555481.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9702..11033
                     /locus_tag="IXF98_001155"
     CDS             9702..11033
                     /locus_tag="IXF98_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1555482.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11048..12010
                     /locus_tag="IXF98_001156"
     CDS             11048..12010
                     /locus_tag="IXF98_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1555483.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12121..12459
                     /locus_tag="IXF98_001157"
     CDS             12121..12459
                     /locus_tag="IXF98_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1555484.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12538..13245
                     /locus_tag="IXF98_001158"
     CDS             12538..13245
                     /locus_tag="IXF98_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1555485.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13348..14829)
                     /locus_tag="IXF98_001159"
     CDS             complement(13348..14829)
                     /locus_tag="IXF98_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1555486.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15138..15674
                     /locus_tag="IXF98_001160"
     CDS             15138..15674
                     /locus_tag="IXF98_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1555487.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15752..17122
                     /locus_tag="IXF98_001161"
     CDS             15752..17122
                     /locus_tag="IXF98_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1555488.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17189..17524
                     /locus_tag="IXF98_001162"
     CDS             17189..17524
                     /locus_tag="IXF98_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555489.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17605..18462)
                     /locus_tag="IXF98_001163"
     CDS             complement(17605..18462)
                     /locus_tag="IXF98_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1555490.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18569..19084
                     /gene="tadA"
                     /locus_tag="IXF98_001164"
     CDS             18569..19084
                     /gene="tadA"
                     /locus_tag="IXF98_001164"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1555491.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19107..19191
                     /gene="ffs"
                     /locus_tag="IXF98_001165"
     ncRNA           19107..19191
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXF98_001165"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19178..19432
                     /locus_tag="IXF98_001166"
     CDS             19178..19432
                     /locus_tag="IXF98_001166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555492.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            19401..20786
                     /locus_tag="IXF98_001167"
     CDS             19401..20786
                     /locus_tag="IXF98_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1555493.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20823..21500
                     /locus_tag="IXF98_001168"
     CDS             20823..21500
                     /locus_tag="IXF98_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1555494.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21508..22272
                     /locus_tag="IXF98_001169"
     CDS             21508..22272
                     /locus_tag="IXF98_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1555495.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22274..22933
                     /locus_tag="IXF98_001170"
     CDS             22274..22933
                     /locus_tag="IXF98_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1555496.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22963..23472)
                     /locus_tag="IXF98_001171"
     CDS             complement(22963..23472)
                     /locus_tag="IXF98_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1555497.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23584..24582)
                     /locus_tag="IXF98_001172"
     CDS             complement(23584..24582)
                     /locus_tag="IXF98_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1555498.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24783..25367
                     /locus_tag="IXF98_001173"
     CDS             24783..25367
                     /locus_tag="IXF98_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1555499.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25444..26619)
                     /locus_tag="IXF98_001174"
     CDS             complement(25444..26619)
                     /locus_tag="IXF98_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1555500.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26701..27081
                     /locus_tag="IXF98_001175"
     CDS             26701..27081
                     /locus_tag="IXF98_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1555501.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27155..27517
                     /locus_tag="IXF98_001176"
     CDS             27155..27517
                     /locus_tag="IXF98_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555502.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9117 a         4705 c         5812 g         8311 t
ORIGIN      
        1 ttttgcaatg aaattcaatt tttcaatgat tgtaaaatga aaaaaacagg acgagatttt
       61 ctctaatcta aaaataaaaa aagaatttta aggaaaaaca gagaaataaa atacttaata
      121 taaatggaaa gagaaaaaaa taatcataca taggagaaaa aaaacaaaat agaaaaaagt
      181 gattttctaa taaattattc atgtggaaat gtcattttta ctgtataatt catttgatta
      241 tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat ttgaagaaaa
      301 caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc catacgtatg
      361 tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt ccagcaggaa
      421 agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt actcgtactg
      481 ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat gtgtttgatc
      541 aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt ccggaaaaag
      601 gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa
      661 tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat gatacttcta
      721 aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc tttggtttct
      781 taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct gtagggatcg
      841 ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg
      901 acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta gctttaccgc
      961 tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa gaggtaattc
     1021 cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg atcgtagcag
     1081 taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg agcccgttta
     1141 gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg acattccgat
     1201 tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata tatgcagaaa
     1261 aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat ttccgttctc
     1321 atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa ttgatcttat
     1381 tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa ggctattaca
     1441 ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc attgctgttc
     1501 ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa tctggtgcag
     1561 ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt cttgtcttgg
     1621 gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg atcgggacgg
     1681 cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc caaagtttgt
     1741 tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca atcatggctc
     1801 cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat cagcttggtt
     1861 caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg ctaggtgttg
     1921 cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg
     1981 ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag tactggagac
     2041 ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt cattttacgg
     2101 taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc tatcatcagt
     2161 tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa gtgtttgcac
     2221 cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg gctgtagaag
     2281 aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt gagggaaaag
     2341 tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga ggagaaggcg
     2401 gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct ggtgtaacga
     2461 cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg ttgatcagca
     2521 aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt tcgtaccgat
     2581 tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt gataaaatta
     2641 tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt tttgaagagt
     2701 tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa gctgggatca
     2761 tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga atcgtagaag
     2821 aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca ccagaattat
     2881 ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact gctagcgaag
     2941 atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca ctgaaacgat
     3001 tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga caaggaagct
     3061 tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt gccaaggcat
     3121 tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa atcgccttac
     3181 gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa
     3241 ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt gatacagtta
     3301 tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg actttcgaaa
     3361 aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc tgtttgtctg
     3421 tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag gaggaagcac
     3481 aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa
     3541 agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga aagcacaatc
     3601 actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca aagacaagtt
     3661 ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca agcaaataaa
     3721 aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga tcattatcct
     3781 gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac ttcatcacag
     3841 ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg ttcatgggcg
     3901 atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt tccagtaggc
     3961 tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc gactcgctgc
     4021 tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc aattcctaca
     4081 cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt agtgatttcc
     4141 attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta agcttggaaa
     4201 ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag tgaacgatct
     4261 tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga aagaaaagtc
     4321 gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc gaaattcagt
     4381 tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc
     4441 cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa tcttaatatc
     4501 cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc ggctaatagt
     4561 cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga aacagcaaat
     4621 ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg tctgttacct
     4681 ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca
     4741 agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc aactcctaat
     4801 gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt acttctggat
     4861 tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt aatcataatg
     4921 atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt tttagaaaaa
     4981 cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt tgattgtaag
     5041 ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc cattttatct
     5101 ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt cccatttagt
     5161 tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa gttgaatttc
     5221 atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt catttcataa
     5281 gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc caggtcattt
     5341 gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt
     5401 ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg tgcagttgct
     5461 gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc cattcctcca
     5521 gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc agaactagtg
     5581 atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga ataactcttt
     5641 acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt aagagaaaaa
     5701 ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt
     5761 ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc tttttgtgct
     5821 tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac aaaaataaga
     5881 atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt tgtacttttc
     5941 catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat ttgataatag
     6001 acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca acatgaggtt
     6061 ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat
     6121 gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct agatgctaca
     6181 gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat ttagtttatt
     6241 tgttgtataa taatggggaa cagctgaata cttatataag tattctggct atagcgtttc
     6301 gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc caactgtcag
     6361 aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa tagtgatttt
     6421 gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg acatctttaa
     6481 acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat cccattaggc
     6541 ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga tatttttatt
     6601 ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct
     6661 gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt aacacatgga
     6721 catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt accagtattc
     6781 gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa
     6841 ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg tgaaacagta
     6901 gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa tctgaaaaca
     6961 aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc tatcccaatg
     7021 tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt agccttacta
     7081 agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca aatagcagat
     7141 gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt
     7201 aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg aaaagttgtc
     7261 ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga aaaacttcaa
     7321 ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga tccagaagaa
     7381 ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca aaaaatggca
     7441 aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat cactactacg
     7501 ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta tcgagctggt
     7561 ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa tcctaatgac
     7621 ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca aggagagtat
     7681 cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata caaaaatatc
     7741 tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag tgttgcagga
     7801 actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat cggaaacatt
     7861 gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt tgtcactatc
     7921 agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg attcgtttac
     7981 gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac aatcgttgag
     8041 aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat ccgagatcaa
     8101 ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc agtcattatg
     8161 gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac ctctctatag
     8221 agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca gcatccctcg
     8281 ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa
     8341 cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca tgtggtagaa
     8401 gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat atgtaaaact
     8461 ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt gaacgatcac
     8521 ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt tcctggaatg
     8581 gattttgtat taatgataca ggatatcccc acggagcaaa cacccctatt ggaataaaac
     8641 tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa
     8701 ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga
     8761 tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga
     8821 gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg
     8881 ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata
     8941 ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg
     9001 cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga
     9061 aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga cagacttaac
     9121 tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg
     9181 agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc
     9241 tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc
     9301 gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga
     9361 cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac
     9421 gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt
     9481 cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc
     9541 agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt
     9601 ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa
     9661 aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt
     9721 tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg
     9781 caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg
     9841 tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa
     9901 aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag
     9961 gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac
    10021 atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag
    10081 tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga
    10141 ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg
    10201 atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag
    10261 atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga
    10321 cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag
    10381 tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat
    10441 cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa
    10501 tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg
    10561 tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc
    10621 agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata
    10681 tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg
    10741 tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa
    10801 tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg
    10861 aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc
    10921 cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa
    10981 acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagcag
    11041 gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg ataaaatcat
    11101 tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag gattagccga
    11161 actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg
    11221 gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt
    11281 catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc agttggcaga
    11341 aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc ttgtgtgggt
    11401 aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga tgtttcctga
    11461 atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg tcggagatac
    11521 agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt cgatattgag
    11581 aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt
    11641 cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag
    11701 aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag agacagcttc
    11761 gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt ttgttcagca
    11821 gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca atgaattggc
    11881 taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa aagatttagc
    11941 agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata agagcagaaa
    12001 aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt
    12061 ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag gagaaagaag
    12121 atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga tcgaggcttg
    12181 ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa
    12241 gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca
    12301 gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc
    12361 aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga aggatttgga
    12421 ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa ggtcttttca
    12481 cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga aacgaaaatg
    12541 tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat tcttggatta
    12601 gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata cgatcaagtg
    12661 agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca aagtcaacag
    12721 ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta caattcaggt
    12781 aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc tgcgataggt
    12841 gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt cccattgaag
    12901 attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt ccccttgatt
    12961 atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat tttcgctttt
    13021 tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc gtctcggcga
    13081 gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc gatggcaaga
    13141 cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac attcctgcag
    13201 ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa agaagttgtg
    13261 aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac catttatttg
    13321 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
    13381 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
    13441 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
    13501 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
    13561 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
    13621 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
    13681 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
    13741 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
    13801 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
    13861 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
    13921 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
    13981 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
    14041 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
    14101 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
    14161 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
    14221 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
    14281 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
    14341 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
    14401 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
    14461 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
    14521 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
    14581 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
    14641 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
    14701 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
    14761 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
    14821 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
    14881 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
    14941 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
    15001 aagatattct tgtttttaag gcggaaaaat tgatttttaa tggatataac taaatatgtt
    15061 ttgataatta tcaataatat ttttagaatt atttatgtta tttatttatg ggtagatgaa
    15121 aagagacgga ggtaagaatg atggggagag taacaaatta tagtaaagaa tatttgctgt
    15181 tcaaaagtat ggtatacgtg gaagaatatg gaatgaagtc aattactgca agagagttag
    15241 cagagttctg cggttgttct acatacccga tttatacaca tttcaaatca atcagcgggt
    15301 taaaagagaa gatattagaa gaaatcactg tttattttga aaactactta gctgaatgca
    15361 gttccgatga tgttttaagc attgtctact tattgaaaga ttttttttct atccatgaaa
    15421 gtattcgtga aatcattgca aaatctgatt tagacatgga ttcgctattt aagcggccat
    15481 ttttctcata tgtcaaaggt cacacgaaga tcaaagaaga aaagtcgctt gaattggtat
    15541 ggcttaatgc attgggaagt ttgcgctcag atgattcttc tcaagatttt cttgaaatga
    15601 tgaacgctgt ttgggataaa aacgaggtga cggatgaata cttggaaaat atcttaaagg
    15661 aacagtcacc ttaggcagca acatgaaaaa gtagatcagc cacctttatt tttcgtaaaa
    15721 aagattgtaa aatgtctaac gaaacattgg aatgaaggag gaagcgacaa cgttaccaca
    15781 gcaatttatc aaaaaatatc gtcttctttt aggcgaagaa gcatccgatt ttttttctgc
    15841 gcttgaacaa ggaagcgtga aaaaaggatt tcggtggaat ccactaaaac cggcaggatt
    15901 ggacatggtc caaacttacc atagcgaaga attacaaccc gctccctaca gcaatgaggg
    15961 atttcttggc actgtcaatg gaaagtcttt cctccatcaa gctgggtatg aatatagcca
    16021 agaaccaagt gcaatgatcg ttggaacagc tgctgcagca aaacccggtg aaaaagtttt
    16081 ggatttgtgt gcggctcccg gtggtaaatc aacccaatta gctgctcaaa tgaaggggaa
    16141 agggttatta gtgacgaatg agatctttcc gaaacgtgcg aagattttat cagaaaatat
    16201 agaacgatgg ggagtttcga acgcaatagt gacaaatcat gcacccgcag aacttgtccc
    16261 tcatttttcg ggcttttttg atcggatcgt ggttgatgct ccttgttcag gagaaggaat
    16321 gttccgaaaa gatccaaatg caataaaaga gtggacagaa gaatctccgc tatactgtca
    16381 aaaacgtcag caagagattt tgtcttcagc aataaaaatg ctaaaaaata aaggacaact
    16441 gatttattcg acttgtacgt ttgcaccaga agaaaatgaa gagatcatta gctggcttgt
    16501 agaaaattat ccagtaacga ttgaggagat tcctttgact caatccgtta gttcaggacg
    16561 ttctgaatgg ggaagcgtag ctggtttgga aaaaacaata cgtatatggc ctcataaaga
    16621 tcagggtgaa ggacattttg ttgcaaagct gacttttcat ggacaaaatc agatgcataa
    16681 agaaaagaag acacgaaaaa aaagtaaagt gcaaatgaca aaagagcagg aaaaactctg
    16741 gacggagttt tcaaatgatt ttcattatga agctactgga agacttttag tgttcaacga
    16801 ccatctttgg gaagttcctg agttagcccc ttctctcgat ggtttaaaag tcgtacgtac
    16861 aggtttgcat ttaggagatt tcaaaaagaa tcgatttgaa cctagctatg cattagcgct
    16921 tgctacaaaa aagatagaga atataccttg cttgccgatc actcaaaagg aatggcaatc
    16981 ttacacagca ggagaaacat ttcaacgtga tgggaatcaa ggatgggttt tgctagtatt
    17041 agataagata cctgtcggat ttgggaaaca agtaaaagga acagtaaaga actttttccc
    17101 taaaggattg agatttcact gagtggatta gttcgtttat ttttttgtct atgctatgat
    17161 ggagatagat aaaaaggagc tgatacttat gaaaacatca acaaaagtaa caatcggatt
    17221 aagcgtagca gccgctgcca gtgtagcgac agcagtcgtt gtatcgggaa aagtaatgga
    17281 aaagatccat catatgacta ctcggacgaa agtaaaaaaa ttcgtctatg acaaatttga
    17341 tggaaatgaa aaattactag aaatcgtaga tggtctttca gatagtgact tggattcttt
    17401 gatgggaatg ttagcaaaaa tcaaatcagg aaaaaagaaa atctctgttt atggtgattc
    17461 aatcaaagat tcaaccgaag atgtaaaaga ccggctaatg cattttgttg aaaaaatgat
    17521 gtaagagaaa ctagcttttg aacactattc attcgaaata aggggttgtg acaaaattat
    17581 tgtcacaacc cctttaatct atctttaatt atgttcatga agcaataaaa gatagcaaaa
    17641 cagtaaatcg ttggcttttt ttagattgaa accagactgt tcttgaaatt tttcgattcg
    17701 atattttaga gtattacgat gaagaaacag tgtttttgca gcagagctga cattgcctaa
    17761 ttcattccat aattcttgaa tgatcacttc catttcattt tgtttgatca gttctatgta
    17821 acttttgatc aacttactat cttttacagt atcttttgta tagtaatgta aagccacatc
    17881 ttgcatagaa aaagtacggc tgtgagtata aagattgttt tgctctgtta aaaaaatttt
    17941 tctttcttca gcaaacaaag ggacgaggtc ggtgccaggt gaatggaaag aaccaacgaa
    18001 tacagacgtt gtcgtatcaa aatcagcatc taaagataga aaaataccgt tgatttcagt
    18061 agcatcaaga taatcacggt ttatttgttc gactaagagc gcatctgtgt ctgtataaaa
    18121 aaagaaatca gcagaatagc gaaataattc caaaatagta tcttgccatt ctttttttaa
    18181 gaattctcct tttgattcga cgtgaagctg gatcaaacga aagctttttt cttgaacaca
    18241 agaagcatct ttaaaaagat agtgatacca tgggtgtttt ttttgatcgt tgattacagg
    18301 gaataacgct ttcaaaaggc tggtttcttg ttgagataat gaatcttgtt tgatccagat
    18361 aaaagcatca tcaattgcaa aagaaaggat atccgcttct gatgctggag agtttttttt
    18421 ctgtgcttgt gggtaaagag caagtaattt atccattttc atcttgttca cctcttgtat
    18481 ttattttacc atagaaaaaa aagttagtta cagccttttt atcatatgct aaagtaaaac
    18541 aagaaaaaac aaaggcaggg aagggtaatt ggtaaataca aatcttgatg cagtagaaaa
    18601 agaaaaatgg atgcgccttg cattggcaga agcaaagaaa gcagaaatgc ttcatgaagt
    18661 tccgattgga gcagtggttg tattggatgg aaaagtgatt ggaagaggat ataaccttag
    18721 agaaaccacg caagatgcta ctacacatgc agaaatgctt gcaatcaagg aagcctgtga
    18781 aaaagtggga agctggcgct tggaagatgc tgcacttttt gttaccttag agccttgtcc
    18841 tatgtgcagc ggcgcgatga ttttgtctcg tgtagcagaa gtttatttcg gcgcgtatga
    18901 tccaaaaggc ggaacagccg gtaccttgat gaatcttttg gaggacgagc ggtttaacca
    18961 ccaagcatat gtagaaggtg gcattttaga agacgaatgt gggatgattt tgactgactt
    19021 tttccgaaag ctccgccagc gaaaaaagaa attaaaaaaa gactggtcaa cgaatgaaaa
    19081 ataaggtata ctattctttg ccgaaaggct aggacaatgg tgggcttgtg aagcgtgtca
    19141 ggtctggaag gaagcagcac taagcaggtt ccgccatgtg tctgattata atgaaacgac
    19201 aacagacttt cgggtctgtt ttttttattt ttatagtact gtaaaaatga atgttcctga
    19261 ttttttagcc agactgattt atcgaatatt gtacttatac aatttgcttc aaggcaggaa
    19321 aaactctgaa atagagcttg tttcaatgga agaaaaagct gcctatggta tgataaagat
    19381 aaagagaggc gagagggtac atggaaaaaa tagtgatttt acatacgaat gatctgcatt
    19441 ctcatttaga aaattggcca aggatcagaa ggtttttgga tcaacgtaaa cgagaaaacg
    19501 aaaagaaaga aaaaacgaca acgattgcag tagatctagg tgattttgtg gatcgttggc
    19561 atccattgtc agaagcaacg aatggacagg aaaatatctt gctgatgaat gaagttggtt
    19621 atgatgcagc gacgatagga aacaatgaag gcgtaggaaa ttccaaagaa gagttgaatc
    19681 atttatacga tcatgctgag tttgatatca tcctggataa cttattcgat aaaaatacgc
    19741 tccagccacc ggcatggacg aaaccatata aaatcattac aacgaaacac cagacgaaaa
    19801 tcggactaat tgcatttact gctccatttc cgcttactta taatccgaat ggctgggaca
    19861 tacggaatcc ttatgatatt ttgccagaat tagtcgaaga tttacgttca caagtggatg
    19921 ttttagtttt gatgagtcat ctcgggattc aggatgattt ccaaattgca aaagaaatac
    19981 cagcaattga tgttatttta ggatcgcaca cccatcattt attcagagaa ggtcacgtaa
    20041 tcaatgatgt ccaaatagca gccgctggaa agtacgggca gtatgtcggt gaagtccatt
    20101 taaccatcga tgaagagaaa aaaatcctta agcattcagc caaagccatc cctaccgaaa
    20161 caatgacagc ttttacagaa gatgaacaag aaataaatag ttatctagaa cgtgggcatg
    20221 aattattaaa agaaaaagaa gtagcagatt tgccattttc tctgagtcta gatatcttca
    20281 atgagcattc gtttatccaa gtagcctttg aggcggtgaa agaacgagga aagacggaag
    20341 cagcaatatt aaacagtggt ctgtttttaa ctgatattcc aaaagggcgg gtcaatcagg
    20401 atcagcttca cactgcgttg ccgcatccga tgcacttgtt aaatgtcaca ttgggtggaa
    20461 atgatctgat ccgcttagtt ttagaaatag aaaaaaatcg taacttttta cgtaactatc
    20521 ctatgaaggg aatgggattt cgcggtaaga tttttgggca gatggtctat aatggaatca
    20581 catatgattc tattaaccat caagtctttt ggaagaacaa gccaatcgat ctgaataaaa
    20641 attatacttt tactaccgta gatcatttga tgtttgttcc attttttcca acgattgaga
    20701 ttgctggaaa aaatgaattt ttgttccctg aatttatccg aagtgtggta ggagattact
    20761 tgaaagctca ctacccaatc aaataaaaac aaggtataat aaaaaagata ggtggtgaac
    20821 gcatgtctgc aaaacaaaat gaacagcaga acgttgagca taaaacagaa attgctaaga
    20881 aaaaatcatc agctgaacag gaaacaacag tcaccgaaga actgacagct gctttggaag
    20941 aaatcgttga ggagcctaaa gcagaaaaag tcaaaggcga gctagtgaca ctgttagatg
    21001 acagtaattt tttgataggt gaaagacatt atcggcttgt gtccgattat cgtgaaggat
    21061 ttaatgcaga aaagcttgga gaacgttaca gtgatgtact agcccgatat gattatatcg
    21121 taggagattg gggttatgaa caactacgcc taaaaggatt tttccaagcg gataatcgac
    21181 gggcgcatcc agatcagcga atcgattcct tggaagacta tctttacgaa tattgcaatt
    21241 ttggttgtgc gtattttgtc atcgagagaa tcggcggtaa aaaagaaaag acaactcagc
    21301 gccgaaaaaa gaaaaaaaac cataatcgtg cgtatatcga tgagaaaaaa ggaccagtcg
    21361 catctaatcg caaaaaaccg gttattaaaa atcgaaaaac agagtcagca aaaacctctg
    21421 aaaaagtaaa aaaacaagaa caacctaaac aagtagaaaa aacaaaaaca gcatttacga
    21481 tcagaaaaag ggaagagtag tggatgaatg gactataaag gatatttaat tgatttagac
    21541 ggaacgattt atcgtgggac acaacccata ccggccggta aacgatttgt ggaagaattg
    21601 caaaaaagaa aattaccttt cttgtttgtt accaataaca cgactaagtc accagaaaca
    21661 gtagctaatc gtctagcaga tgagtttgac attcatgtag ctccagagac agtctacaca
    21721 gctactttgg caacaatcga ttttatgaaa gcagatggaa aagggaaaaa agtctatgtt
    21781 atcggagaag caggattgat tgacttgatt ttagctgccg gatttacatg ggaagaagag
    21841 gctcctgact atgtagtggt aggcttagat aattatctga cctatgaaaa agtagttaaa
    21901 gcaacacttg ctattcaaaa aggtgctact ttcattggaa cgaacccaga taagaatata
    21961 cctactgaac gcggccttct tcctggagca gggtcagtca tctcttttgt agagacagca
    22021 acccaaacgc cgccgatcta catcggaaaa ccagaagcca tcattatgga taaagccgtt
    22081 gaagtattag ggctgcaaaa agaagaagta atcatggttg gagataacta tgaaacagat
    22141 atccaagctg ggatacgcaa taatatagat actttgctcg ttttatcagg ttttacaaaa
    22201 gaagaagatg tacctgggtt gcctatccca gctacctatg tgaaacaatc actggatgaa
    22261 tggagcttct aatatgaaaa attcgagatg gcagtggatg gaatatgctg gattgttcag
    22321 cctatttctg acactgatca gtctggcagt tggtgtgacg atcaactttc gaccactata
    22381 cgtatttgat atcggacatt tgcaaatttt ggattatact tctttagatc aagagacgtt
    22441 actgaaaaat tttgatcacc taatgaatta tctcaataat cccttcaaaa ccatcctcag
    22501 cttgccagat ttcccagttt ctgctagcgg agcccatcac ttttatgaag tgaaaatttt
    22561 gtttttagtg gattatgctg tgtttttcat cactttgatc ccgagtattt tgtttatcaa
    22621 atatttgcaa aagaatgatc ggctttggcg gttgattcgt ccgtttcaaa tcggcatgtt
    22681 actgccagtt gttttcggtt ttttcatgat gataggattt gatcgctttt ttatcctttt
    22741 tcatgaaacg tttttcaaca acgatgactg gttattcgat cccgttactg atccaatcat
    22801 caatgtactt ccagaagaat ttttcatgca tagctttatc ttattttttg tattattaga
    22861 attatttttt gctgtttttc ttttcttagg aaaaaatagc ttgaaacaaa caaaaaagaa
    22921 agaactcgtc taaatagacg ttttctttct ttttttgttg aattattgag taaggcgact
    22981 tcttttttct ggttctttgt gtgctaatcg actggcagca gcagcggcga tcgctccgac
    23041 gatatcatct aagaaggtat ggacatgaac gccatcgtgg ctgtttagtt cttttaaaat
    23101 accaggtttc accttgtcga tataaccata attcgtaaag ccgatcgtac cgtaaacatt
    23161 cacgattgac aaggctagga tctcatcgat accgtaaagg ccttcgtcat cttcgataat
    23221 atgttgcaac ggactcatca attttttctt ttctgctaat atatctaatt ggattccagt
    23281 gataatggcg ttatgtactt cacgctttgt tagcacgctg ttgacacttt cagtacaatc
    23341 cgccaaagtc aaatcagaaa tatagtcttt ttgtaaaaac atgacaagtt gtgcgatatc
    23401 ttctacagtt acgccacgtt ccttcaatag ttctcttgcc ttttcttcta aagtctcagt
    23461 tttgacgacc atattattgc tcccccttta ttttttcttt ctctatttca tgttaacgga
    23521 acatgaaaaa aatgcaagaa actgctttcg ggtttaactt gaaatgcaaa gagatataaa
    23581 aaatcaacta ggacgaagat ttttatcgta taagcttgtg ctgtgaccag gctgagttcg
    23641 ttcctttgga ttaatatagt gtaaagcgtt gttcactgca gtaggtgctt caccgagccc
    23701 agtagcaatc agtttcactt ttccttcata ggtacagatg tcaccagctg cataaacgcc
    23761 tggaatagaa gtagacatat ctgattttac agtaatggca tttctggtac tatcaagtcc
    23821 ccaagaagaa agatgttcta aattagaaga gaatccataa ttgacgatca gagaatcaat
    23881 catcaaatca atcgtctcgt cacttttcac tttttttaac cggatatctg tcagttcccc
    23941 attattgcct gataatccat cgatgagata gggagtaagc aagttgacag atgaagattt
    24001 caagcgtgat acactatgtt cgtgcgcacg aaattccggg cgacgatgaa tcaagtaaac
    24061 ttcgcttgca ataggctcta acatcaacgt ccaatcgata gcagaatcac cacctccagc
    24121 aatagcgact ttttttccag catacttcat caaatcattc acgaaataat ccaatccgtg
    24181 gttttcaaat gattcagcat tatctaaatt caattttctt ggctggaatg aaccatttcc
    24241 taaagctaaa atgactgctt tggaataatg gatgccttta tttgtagtaa tctcgataac
    24301 ttcgtcttca cgagtaagcg agagaacttc ttcttttaaa tggaatgtat ggttaaaagt
    24361 agttaattgt ttttctaatt gatcgattaa ttcacttgct tttattgctg gatacccagg
    24421 aatatcgtaa atatattttt cgggatacaa agttgctaat tgaccaccaa gttgaggtag
    24481 actgtcaatg atttttgttt tagcttggcg cattcctgcg taaaaagcgg caaacatccc
    24541 tacaggacct gcaccgatga ttgtaatgtc atagatttcc ataaaaattc ctcccttttc
    24601 acactatagc agaatttaat tgagaatgct tctcaaaaat gtttgaattc gaaatcgaaa
    24661 cggtaagtta tagaatgtat ctagtgatga aaaataaaag aaaacaaatc tagtattttt
    24721 acgagaggtt aggtattatg ttattattat tatgaagtat gcaaatatta ggaggaaaca
    24781 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
    24841 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
    24901 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
    24961 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
    25021 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
    25081 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
    25141 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
    25201 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
    25261 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
    25321 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
    25381 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
    25441 gatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
    25501 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
    25561 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
    25621 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
    25681 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
    25741 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
    25801 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
    25861 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
    25921 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
    25981 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
    26041 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
    26101 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
    26161 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
    26221 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
    26281 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
    26341 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
    26401 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
    26461 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
    26521 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
    26581 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
    26641 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
    26701 atgacttata aaattggaca aataattgaa ggaaaagtga cagggataca gccttatggc
    26761 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
    26821 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
    26881 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
    26941 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
    27001 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
    27061 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
    27121 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
    27181 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
    27241 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
    27301 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
    27361 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
    27421 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
    27481 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
    27541 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
    27601 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
    27661 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
    27721 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
    27781 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
    27841 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
    27901 agttcttgac aaataacaaa cgataagtta tgataaatga gttgc
//
LOCUS       DACETN010000030        26517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DACETN010000030 DACETN010000000
VERSION     DACETN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.031"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(99..1229)
                     /locus_tag="IXF98_001177"
     CDS             complement(99..1229)
                     /locus_tag="IXF98_001177"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1555503.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IXF98_001178"
     CDS             complement(1878..3080)
                     /locus_tag="IXF98_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1555504.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IXF98_001179"
     CDS             complement(3140..3343)
                     /locus_tag="IXF98_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1555505.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="IXF98_001180"
     CDS             complement(3485..4552)
                     /locus_tag="IXF98_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1555506.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            4879..5691
                     /locus_tag="IXF98_001181"
     CDS             4879..5691
                     /locus_tag="IXF98_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555507.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IXF98_001182"
     CDS             complement(6715..6867)
                     /locus_tag="IXF98_001182"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555508.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IXF98_001183"
     CDS             complement(7058..7936)
                     /locus_tag="IXF98_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1555509.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IXF98_001184"
     CDS             complement(7975..9093)
                     /locus_tag="IXF98_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1555510.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IXF98_001185"
     CDS             complement(9090..11189)
                     /locus_tag="IXF98_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1555511.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IXF98_001186"
     CDS             complement(11240..12061)
                     /locus_tag="IXF98_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1555512.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IXF98_001187"
     CDS             complement(12058..12867)
                     /locus_tag="IXF98_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1555513.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IXF98_001188"
     CDS             complement(12888..13382)
                     /locus_tag="IXF98_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1555514.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IXF98_001189"
     CDS             complement(13394..13882)
                     /locus_tag="IXF98_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1555515.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IXF98_001190"
     CDS             complement(13959..16655)
                     /locus_tag="IXF98_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1555516.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IXF98_001191"
     CDS             complement(17083..18156)
                     /locus_tag="IXF98_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555517.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IXF98_001192"
     CDS             complement(18153..18980)
                     /locus_tag="IXF98_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555518.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IXF98_001193"
     CDS             complement(18981..19787)
                     /locus_tag="IXF98_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555519.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IXF98_001194"
     CDS             complement(19799..20884)
                     /locus_tag="IXF98_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555520.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IXF98_001195"
     CDS             complement(20901..21443)
                     /locus_tag="IXF98_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555521.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IXF98_001196"
     CDS             21701..22690
                     /locus_tag="IXF98_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1555522.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IXF98_001197"
     CDS             22687..23424
                     /locus_tag="IXF98_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1555523.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IXF98_001198"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IXF98_001198"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1555524.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IXF98_001199"
     CDS             23988..24620
                     /locus_tag="IXF98_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1555525.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IXF98_001200"
     CDS             24705..25352
                     /locus_tag="IXF98_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1555526.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IXF98_001201"
     CDS             25357..26340
                     /locus_tag="IXF98_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1555527.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8113 a         5598 c         4496 g         8310 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttt
//
LOCUS       DACETN010000031        26446 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DACETN010000031 DACETN010000000
VERSION     DACETN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.032"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(148..843)
                     /locus_tag="IXF98_001202"
     CDS             complement(148..843)
                     /locus_tag="IXF98_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1555528.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(933..2033)
                     /gene="ychF"
                     /locus_tag="IXF98_001203"
     CDS             complement(933..2033)
                     /gene="ychF"
                     /locus_tag="IXF98_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1555529.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2042..2233)
                     /locus_tag="IXF98_001204"
     CDS             complement(2042..2233)
                     /locus_tag="IXF98_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1555530.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2254..3144)
                     /locus_tag="IXF98_001205"
     CDS             complement(2254..3144)
                     /locus_tag="IXF98_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1555531.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3131..3898)
                     /locus_tag="IXF98_001206"
     CDS             complement(3131..3898)
                     /locus_tag="IXF98_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1555532.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3910..4623)
                     /gene="rsmG"
                     /locus_tag="IXF98_001207"
     CDS             complement(3910..4623)
                     /gene="rsmG"
                     /locus_tag="IXF98_001207"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1555533.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4855..6756)
                     /gene="mnmG"
                     /locus_tag="IXF98_001208"
     CDS             complement(4855..6756)
                     /gene="mnmG"
                     /locus_tag="IXF98_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1555534.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6773..8170)
                     /gene="mnmE"
                     /locus_tag="IXF98_001209"
     CDS             complement(6773..8170)
                     /gene="mnmE"
                     /locus_tag="IXF98_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1555535.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8514..8852
                     /locus_tag="IXF98_001210"
     CDS             8514..8852
                     /locus_tag="IXF98_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555536.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9020..9778)
                     /locus_tag="IXF98_001211"
     CDS             complement(9020..9778)
                     /locus_tag="IXF98_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1555537.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9796..10614)
                     /locus_tag="IXF98_001212"
     CDS             complement(9796..10614)
                     /locus_tag="IXF98_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1555538.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10611..10967)
                     /gene="rnpA"
                     /locus_tag="IXF98_001213"
     CDS             complement(10611..10967)
                     /gene="rnpA"
                     /locus_tag="IXF98_001213"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1555539.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11085..11219)
                     /gene="rpmH"
                     /locus_tag="IXF98_001214"
     CDS             complement(11085..11219)
                     /gene="rpmH"
                     /locus_tag="IXF98_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1555540.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11743..13077
                     /gene="dnaA"
                     /locus_tag="IXF98_001215"
     CDS             11743..13077
                     /gene="dnaA"
                     /locus_tag="IXF98_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1555541.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13275..14405
                     /gene="dnaN"
                     /locus_tag="IXF98_001216"
     CDS             13275..14405
                     /gene="dnaN"
                     /locus_tag="IXF98_001216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1555542.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14634..14876
                     /gene="yaaA"
                     /locus_tag="IXF98_001217"
     CDS             14634..14876
                     /gene="yaaA"
                     /locus_tag="IXF98_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1555543.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14863..15987
                     /gene="recF"
                     /locus_tag="IXF98_001218"
     CDS             14863..15987
                     /gene="recF"
                     /locus_tag="IXF98_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1555544.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15984..17930
                     /gene="gyrB"
                     /locus_tag="IXF98_001219"
     CDS             15984..17930
                     /gene="gyrB"
                     /locus_tag="IXF98_001219"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1555545.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17950..20421
                     /gene="gyrA"
                     /locus_tag="IXF98_001220"
     CDS             17950..20421
                     /gene="gyrA"
                     /locus_tag="IXF98_001220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1555546.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20657..20956
                     /locus_tag="IXF98_001221"
     CDS             20657..20956
                     /locus_tag="IXF98_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1555547.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            21006..21518
                     /gene="ssb"
                     /locus_tag="IXF98_001222"
     CDS             21006..21518
                     /gene="ssb"
                     /locus_tag="IXF98_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1555548.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21544..21780
                     /gene="rpsR"
                     /locus_tag="IXF98_001223"
     CDS             21544..21780
                     /gene="rpsR"
                     /locus_tag="IXF98_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1555549.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21923..23896
                     /locus_tag="IXF98_001224"
     CDS             21923..23896
                     /locus_tag="IXF98_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1555550.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23902..24354
                     /gene="rplI"
                     /locus_tag="IXF98_001225"
     CDS             23902..24354
                     /gene="rplI"
                     /locus_tag="IXF98_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1555551.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24628..25995
                     /gene="dnaB"
                     /locus_tag="IXF98_001226"
     CDS             24628..25995
                     /gene="dnaB"
                     /locus_tag="IXF98_001226"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1555552.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         8024 a         5222 c         5237 g         7963 t
ORIGIN      
        1 actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt tgtcacagtc
       61 ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc ctagatatct
      121 agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac ttccaataat
      181 cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga taatgataac
      241 tactactgga tctaatactg gattgataac tggtgtcaat agtgttgtcg cagaaaacag
      301 tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca ttcctggttt
      361 cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca tcatataaaa
      421 gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag ctggcgttgt
      481 tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa gcaacagcaa
      541 cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg ctggcttatt
      601 gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg ttcttccgcc
      661 tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt tttcttcttc
      721 agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt gatttctttt
      781 ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt gttctggttc
      841 cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca aaagacattc
      901 taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa taacattacg
      961 tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc ttcttttgct
     1021 gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc acggataaat
     1081 ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat tccttttttg
     1141 aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa gcctaacaaa
     1201 tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa agcttctaaa
     1261 aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg agcactgacc
     1321 acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac ataatgattc
     1381 gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat tggttttgtc
     1441 gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc gatcgttcgg
     1501 gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag ttctgccact
     1561 gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt gttgatagat
     1621 tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag tggatcaaca
     1681 cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg acaaatagcg
     1741 tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt acttgcgcct
     1801 tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt tttcggatgt
     1861 acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc tacgttgggg
     1921 tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat cgcattaaac
     1981 aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc cataaattta
     2041 ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca aaatctcgac
     2101 gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct gcgcccattc
     2161 gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca acgatatcgt
     2221 gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt gaatattcaa
     2281 gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga tcttaccttt
     2341 cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac gatcttcgct
     2401 ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct tttcttttcc
     2461 atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta aattttcagt
     2521 tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta acagcgtacg
     2581 ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat ccggtaaaga
     2641 aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac tagctacttc
     2701 tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt cttctaacgg
     2761 attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg cttcttcgtc
     2821 aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg ctcgaaaacg
     2881 tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga tgatgggttg
     2941 aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt cttcaaatgt
     3001 tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga caacggtatc
     3061 atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat cgattcctcg
     3121 gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct tggtagactt
     3181 cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta ggtgcttctg
     3241 ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag tatttacgta
     3301 cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc agtaagactc
     3361 cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta ttcagtaatt
     3421 ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc gaatcacttg
     3481 ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc aaaatataat
     3541 catactgact gcttacttct gctaaagatc cttttaatct tgattctctt gccatcattg
     3601 acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata ctcaaatttt
     3661 cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga acatcataga
     3721 tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt ccttgtgcat
     3781 ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct agattcaccg
     3841 tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt tgtgccatct
     3901 cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg ggtatttctt
     3961 tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga cattcggcaa
     4021 ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt gttttgcttc
     4081 tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt cttgtttagc
     4141 caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag cagtgacaag
     4201 gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt catgatataa
     4261 ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc gtttattcag
     4321 agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta tgctaggaaa
     4381 ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta cttcaaatgc
     4441 taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg taatagctgt
     4501 aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga attgattcat
     4561 ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt cttctggtat
     4621 cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc cctactttaa
     4681 cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg aaaagtaaga
     4741 aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga agagagaact
     4801 agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta cgtattacgc
     4861 tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga tacttaggtc
     4921 tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt ggattttctg
     4981 caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga tgttttctgg
     5041 aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt ttttgatgta
     5101 gccttcatat ttgatttgga cttctacttg ttcgatttct tttcgtgtca attcttcatt
     5161 ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta aaaagtcgct
     5221 tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt cttggacttc
     5281 ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa ctgcttcttt
     5341 tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt gacccatttc
     5401 tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag cacgtgaagt
     5461 cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca tgacaccgat
     5521 gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca aagctgcatt
     5581 gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac catttgtttg
     5641 tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga gctggtgtgg
     5701 ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt tttctaatcc
     5761 agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag ataaaccttg
     5821 aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat gacgtggttt
     5881 gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc ctaccccttc
     5941 aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt ggtgagtcgt
     6001 ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac tatctggtgt
     6061 gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa cagaatagtc
     6121 gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga tagcaaagcc
     6181 aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg gacctgatga
     6241 atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg taataacaac
     6301 tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa caccatcttc
     6361 gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt tttcgatcgt
     6421 atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg ctcggacagc
     6481 tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga tattcttacc
     6541 catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg gtccgcctac
     6601 agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta ataatgtttt
     6661 tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg cgccaacaac
     6721 gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc ttttattttc
     6781 ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg tcgccgacaa
     6841 cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc acaggcattc
     6901 ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct aacaacgcga
     6961 tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg ctgaagaata
     7021 aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg gaaattgaaa
     7081 aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt gctggcaaat
     7141 ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat aattgttcat
     7201 cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct gcttctgcta
     7261 atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt tcccgaattc
     7321 cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct tcgattacat
     7381 ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac aaatggttta
     7441 acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac ccttcccgca
     7501 gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg atcatctgcg
     7561 ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct ggataatcaa
     7621 tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag cggatcaaag
     7681 cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct gtttttgccc
     7741 gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc aagaatgctc
     7801 gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat agctgcaaaa
     7861 tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct tcccgcgtaa
     7921 atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga ttgctcttgg
     7981 gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat cgtttgtttc
     8041 cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa cgaacgatac
     8101 tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat tctaaagtaa
     8161 tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc ccactccacc
     8221 ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga ttaaattctg
     8281 gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta gatgaagttg
     8341 agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct ttgcgttctt
     8401 tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta aaaaaaacgg
     8461 tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa tatgtgagcc
     8521 cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat gtcgttttat
     8581 ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt gtggaatgct
     8641 tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta acaacgaaga
     8701 ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac tgtcattttg
     8761 agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct tatggaacag
     8821 tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa gcggatcaaa
     8881 actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt catcgtatac
     8941 tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt caacgtgatt
     9001 tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact aaataacgat
     9061 aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat gcagcatgga
     9121 tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct gtccgtttta
     9181 ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt tcacggtaat
     9241 tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg aagacttgtg
     9301 ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg ccttgctttt
     9361 gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg acaagagctg
     9421 gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt aaagctgctt
     9481 catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg ttgactacct
     9541 cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct actgtttcag
     9601 aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga cccattccta
     9661 ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg gccaaaccca
     9721 aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa atcggcatta
     9781 gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct taagagcacg
     9841 ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt tgaaagggtt
     9901 gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt ataatgaaag
     9961 accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga aattcatgat
    10021 cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac ttgcaaaagt
    10081 aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta agttcaacca
    10141 taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata acgccatcaa
    10201 gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc cattttctgc
    10261 atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc ttgaactata
    10321 ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca tgctctttgt
    10381 ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata aaatgatccc
    10441 tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat aaacaatata
    10501 acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac cggataagac
    10561 gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct tcacttactc
    10621 ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt ggagcgaacc
    10681 tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc caactccgga
    10741 tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac ttcattgcgg
    10801 tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg aggctgtccc
    10861 ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc cgtatggaac
    10921 acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag ttttctttcc
    10981 ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc tttcatcatt
    11041 tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc tgaaagaact
    11101 tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt actcatacgt
    11161 ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt tcttttcata
    11221 tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt tgatagtata
    11281 acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa acttatccac
    11341 aagattattt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    11401 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    11461 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    11521 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    11581 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    11641 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    11701 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    11761 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    11821 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    11881 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    11941 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    12001 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    12061 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    12121 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    12181 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    12241 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    12301 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    12361 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    12421 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    12481 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    12541 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    12601 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    12661 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    12721 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    12781 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    12841 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    12901 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    12961 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    13021 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    13081 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    13141 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    13201 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    13261 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    13321 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    13381 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    13441 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    13501 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    13561 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    13621 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    13681 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    13741 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    13801 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    13861 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    13921 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    13981 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    14041 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    14101 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    14161 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    14221 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    14281 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    14341 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    14401 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    14461 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    14521 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
    14581 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
    14641 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
    14701 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
    14761 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
    14821 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
    14881 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
    14941 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
    15001 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
    15061 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
    15121 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
    15181 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
    15241 agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
    15301 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
    15361 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
    15421 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
    15481 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
    15541 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
    15601 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
    15661 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
    15721 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
    15781 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
    15841 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
    15901 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
    15961 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
    16021 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
    16081 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
    16141 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
    16201 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
    16261 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
    16321 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgtaaacg
    16381 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
    16441 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
    16501 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
    16561 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
    16621 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatca
    16681 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
    16741 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
    16801 attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga acacatgaat
    16861 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
    16921 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
    16981 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
    17041 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
    17101 aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc agagctcgtc
    17161 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
    17221 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
    17281 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
    17341 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
    17401 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
    17461 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
    17521 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
    17581 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
    17641 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
    17701 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
    17761 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
    17821 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
    17881 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
    17941 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
    18001 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
    18061 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
    18121 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
    18181 taccatcctc atggagaccg tgcgatctat gagtcaatgg tacggatggc acagccattc
    18241 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
    18301 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
    18361 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
    18421 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
    18481 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
    18541 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
    18601 tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt
    18661 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
    18721 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
    18781 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
    18841 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg
    18901 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
    18961 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat
    19021 caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga agcaagagca
    19081 cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
    19141 cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc
    19201 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
    19261 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
    19321 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
    19381 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
    19441 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
    19501 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
    19561 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
    19621 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
    19681 gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc gagtgaaagc
    19741 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
    19801 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
    19861 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
    19921 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
    19981 gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa
    20041 gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt gatcacagaa
    20101 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
    20161 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
    20221 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
    20281 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
    20341 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
    20401 gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa gaataaccga
    20461 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
    20521 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta gagcttgtga
    20581 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
    20641 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
    20701 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
    20761 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
    20821 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
    20881 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
    20941 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
    21001 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
    21061 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    21121 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    21181 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    21241 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    21301 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    21361 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    21421 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    21481 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    21541 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    21601 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    21661 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    21721 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    21781 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    21841 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    21901 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    21961 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    22021 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    22081 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    22141 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    22201 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    22261 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    22321 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    22381 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    22441 tggtatttta tctattgata actatgcaga tgccattgat aaattagatg acaaagagat
    22501 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    22561 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    22621 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    22681 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    22741 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    22801 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    22861 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    22921 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    22981 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    23041 tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    23101 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    23161 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    23221 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    23281 tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    23341 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    23401 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    23461 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    23521 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    23581 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    23641 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    23701 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    23761 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    23821 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    23881 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    23941 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    24001 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    24061 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    24121 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    24181 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    24241 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    24301 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    24361 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    24421 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    24481 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    24541 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    24601 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    24661 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    24721 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    24781 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    24841 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    24901 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    24961 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    25021 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    25081 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    25141 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    25201 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    25261 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    25321 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    25381 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    25441 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    25501 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    25561 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    25621 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    25681 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    25741 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    25801 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    25861 aacgagccaa aaaatgacaa catcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    25921 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    25981 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    26041 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    26101 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    26161 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    26221 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    26281 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    26341 ctccttgtga gtcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    26401 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgaga
//
LOCUS       DACETN010000032        25810 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DACETN010000032 DACETN010000000
VERSION     DACETN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.033"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            9..851
                     /locus_tag="IXF98_001227"
     CDS             9..851
                     /locus_tag="IXF98_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1555553.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IXF98_001228"
     CDS             848..1798
                     /locus_tag="IXF98_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555554.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IXF98_001229"
     CDS             1813..2046
                     /locus_tag="IXF98_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1555555.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="IXF98_001230"
     CDS             2377..2961
                     /locus_tag="IXF98_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1555556.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="IXF98_001231"
     CDS             3092..3760
                     /locus_tag="IXF98_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1555557.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3762..5186
                     /locus_tag="IXF98_001232"
     CDS             3762..5186
                     /locus_tag="IXF98_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1555558.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="IXF98_001233"
     CDS             5204..5773
                     /locus_tag="IXF98_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1555559.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="IXF98_001234"
     CDS             5883..7637
                     /locus_tag="IXF98_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555560.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7591..9363
                     /locus_tag="IXF98_001235"
     CDS             7591..9363
                     /locus_tag="IXF98_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555561.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="IXF98_001236"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="IXF98_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1555562.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="IXF98_001237"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="IXF98_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1555563.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="IXF98_001238"
     CDS             10381..10758
                     /locus_tag="IXF98_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555564.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="IXF98_001239"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="IXF98_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1555565.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="IXF98_001240"
     CDS             10952..11935
                     /locus_tag="IXF98_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1555566.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="IXF98_001241"
     CDS             11965..12087
                     /locus_tag="IXF98_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1555567.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="IXF98_001242"
     CDS             12105..13628
                     /locus_tag="IXF98_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1555568.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="IXF98_001243"
     CDS             13652..13891
                     /locus_tag="IXF98_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555569.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="IXF98_001244"
     CDS             complement(14084..14545)
                     /locus_tag="IXF98_001244"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1555570.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="IXF98_001245"
     CDS             complement(14740..14958)
                     /locus_tag="IXF98_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555571.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="IXF98_001246"
     CDS             15076..16692
                     /locus_tag="IXF98_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1555572.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="IXF98_001247"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="IXF98_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18549..18704)
                     /locus_tag="IXF98_001248"
     CDS             complement(18549..18704)
                     /locus_tag="IXF98_001248"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1555573.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18896..19090)
                     /locus_tag="IXF98_001249"
     CDS             complement(18896..19090)
                     /locus_tag="IXF98_001249"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1555574.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(19000..19332)
                     /locus_tag="IXF98_001250"
     CDS             complement(19000..19332)
                     /locus_tag="IXF98_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1555575.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19846..20037)
                     /locus_tag="IXF98_001251"
     CDS             complement(19846..20037)
                     /locus_tag="IXF98_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1555576.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20131..20517)
                     /locus_tag="IXF98_001252"
     CDS             complement(20131..20517)
                     /locus_tag="IXF98_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1555577.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21211..22416
                     /locus_tag="IXF98_001253"
     CDS             21211..22416
                     /locus_tag="IXF98_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1555578.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22433..22867
                     /locus_tag="IXF98_001254"
     CDS             22433..22867
                     /locus_tag="IXF98_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555579.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23484..>25810
                     /gene="esp"
                     /locus_tag="IXF98_001255"
     CDS             23484..>25810
                     /gene="esp"
                     /locus_tag="IXF98_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1555580.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8896 a         3700 c         4829 g         8385 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
    18181 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
    18361 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
    18421 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
    18481 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
    18541 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
    18601 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
    18661 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
    18721 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
    18781 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
    18841 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
    18901 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
    18961 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
    19021 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
    19081 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
    19141 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
    19201 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
    19261 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
    19321 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
    19381 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
    19441 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    19501 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    19561 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    19621 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    19681 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    19741 cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa
    19801 tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc
    19861 gaatttcgag atagctagat atattgcaag catacattct tgataacaat cttgatcata
    19921 ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc
    19981 aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa
    20041 tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag
    20101 aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta
    20161 aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc
    20221 tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag gttctttcaa
    20281 caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcacat ttaaaacatt
    20341 aatcggttgt ttaaatttta tagtatcttt agtaaacaaa tatggtggga gttgatcaag
    20401 ttgttcatgt ttttgaattt cttcttgttt tttataataa gtattagcgg tgttcaaaat
    20461 gaccttttca aagaatcgaa tcaactgggc atttacgcgt tcgtcgttat ctgacatatt
    20521 gcacacctcc tccattaaga tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa
    20581 tgcaattatg ctgcgctgcg aataacttct ataagctcac atgcattgat aggactgctg
    20641 atgtttattt tcaaaaatgt ttttttagtt gataatggat agttatcttt atacttagaa
    20701 aagaaaattc atatatcttc ttaaatatca aacatgtcct tatggctaag aaaatgtctt
    20761 gagcttttta cgtataataa atcttttcta aaacctcctt ttggataata ttcttattcg
    20821 tgtatagatt gtatttttta tttcttcccc cttcataatt aacaactaga aaagagttcg
    20881 aatagacacg tatttgaacg aaaattttta ttggattccc aaattggaat atttagaaac
    20941 gaaaaattcc tattatctgt atagtgctta gataataacg gatcataata attattgtct
    21001 ttgaactaga tatatgtaaa tagtattcat catgatgaaa aatgatgagt tctcggtgat
    21061 aaaatgtact ttttacggtg gaatatacat gaaagcgata agtggatatt ttataatgaa
    21121 gtaagttagc ttattttgac agaggaatag tattttagat aacgtataaa atactattta
    21181 atgatgtaga aaggaaagag agaaacaaaa gtgttttcag ataaagaagt cttattaaag
    21241 tctcttcatg tttgtttaaa gttacctatc gtggttttta attccgaatt cagtattata
    21301 aaagagtatc gctcagatag aactacttat tttttttatg attataaaaa gttactaact
    21361 gataatatta aaaaaactaa agatttccat ttctttactg gtgattttaa tgagatgttt
    21421 cttctataca tgtttaaaac cagatattac ttatttggtc catttagagc aaataatatt
    21481 gataaagttt tttttaaact aaaaatgaac aatttgaatg ttgcaatggc tgatagagaa
    21541 cgactctata attcattgca aaatttgact ttgtattctt taggagacat tcgagatatt
    21601 ctgattttag ttcattattt ttttactgga aaaatagaag atctattcca tgaaccgtta
    21661 attgaatata cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta
    21721 tcacaaaatt acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat
    21781 gttaaaaatg gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata
    21841 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
    21901 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
    21961 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
    22021 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
    22081 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
    22141 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag
    22201 aatgaaatgc atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa
    22261 ataatgttga aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat
    22321 tcttcacact tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa
    22381 ttaggtttag tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact
    22441 atatcattta cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg
    22501 ggaaatttct gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag
    22561 tgtgttaaat gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc
    22621 tagagaatct atcagtcata ttaatggtag caattttaaa caaacattat ttaatctgat
    22681 taagctagat gctaaaatat caagttattt attctttttg atacatgatg aatttatgga
    22741 ttatcaaaaa ttggatcagt taattgaaag agaaagctgg gaagattatt acgtgggtct
    22801 aactttttca gaaaagttaa acaactacaa gttaatagat tataagtatt tgagtgaaag
    22861 caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga
    22921 aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg
    22981 agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat
    23041 gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat
    23101 ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt
    23161 attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg
    23221 cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt
    23281 tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact
    23341 ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag
    23401 attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat
    23461 tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa
    23521 aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg
    23581 ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa
    23641 tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag
    23701 cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt
    23761 tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg
    23821 aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat
    23881 tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg
    23941 gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt
    24001 ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata
    24061 cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag
    24121 agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc
    24181 atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt
    24241 cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg
    24301 gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca
    24361 caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat
    24421 attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag
    24481 ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg
    24541 tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg
    24601 aaaattatgg cataggttca ataactaata atgatttaac taaacttatc gattttgcta
    24661 atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg
    24721 aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag
    24781 atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg
    24841 gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag
    24901 agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg
    24961 acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc
    25021 aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag
    25081 atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat
    25141 taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag
    25201 aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg
    25261 gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag
    25321 agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg
    25381 taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt
    25441 ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag
    25501 ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt
    25561 caatttcaga tgtctataat aaggacttcg gtaatgcaag tgttgatgca aacactggta
    25621 ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc
    25681 ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa
    25741 gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct
    25801 tcaccgcagg
//
LOCUS       DACETN010000033        25674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DACETN010000033 DACETN010000000
VERSION     DACETN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.034"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            170..514
                     /locus_tag="IXF98_001256"
     CDS             170..514
                     /locus_tag="IXF98_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1555581.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IXF98_001257"
                     /pseudo
     CDS             515..934
                     /locus_tag="IXF98_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IXF98_001258"
     CDS             complement(1026..1376)
                     /locus_tag="IXF98_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555582.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IXF98_001259"
     CDS             complement(1342..2673)
                     /locus_tag="IXF98_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1555583.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IXF98_001260"
     CDS             2929..3495
                     /locus_tag="IXF98_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1555584.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IXF98_001261"
     tRNA            3640..3727
                     /locus_tag="IXF98_001261"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IXF98_001262"
     CDS             4061..5035
                     /locus_tag="IXF98_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1555585.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IXF98_001263"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IXF98_001263"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1555586.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IXF98_001264"
     CDS             complement(7450..7896)
                     /locus_tag="IXF98_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1555587.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IXF98_001265"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IXF98_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1555588.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IXF98_001266"
     CDS             8361..8879
                     /locus_tag="IXF98_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1555589.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IXF98_001267"
     CDS             complement(8956..9912)
                     /locus_tag="IXF98_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555590.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IXF98_001268"
     CDS             10078..10884
                     /locus_tag="IXF98_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555591.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IXF98_001269"
     CDS             10881..11870
                     /locus_tag="IXF98_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1555592.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IXF98_001270"
     CDS             11867..12871
                     /locus_tag="IXF98_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1555593.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IXF98_001271"
     CDS             13003..13545
                     /locus_tag="IXF98_001271"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1555594.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IXF98_001272"
     CDS             13565..14263
                     /locus_tag="IXF98_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1555595.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IXF98_001273"
     CDS             14284..14496
                     /locus_tag="IXF98_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1555596.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IXF98_001274"
     CDS             14496..15458
                     /locus_tag="IXF98_001274"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1555597.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IXF98_001275"
     CDS             15476..15871
                     /locus_tag="IXF98_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1555598.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IXF98_001276"
     CDS             16034..16399
                     /locus_tag="IXF98_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1555599.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IXF98_001277"
     CDS             16396..18207
                     /locus_tag="IXF98_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1555600.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IXF98_001278"
     CDS             complement(18269..18436)
                     /locus_tag="IXF98_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1555601.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IXF98_001279"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IXF98_001279"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1555602.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IXF98_001280"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IXF98_001280"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1555603.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IXF98_001281"
     CDS             21193..22173
                     /locus_tag="IXF98_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1555604.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IXF98_001282"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IXF98_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1555605.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IXF98_001283"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IXF98_001283"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1555606.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IXF98_001284"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IXF98_001284"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1555607.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IXF98_001285"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IXF98_001285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1555608.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5377 g         7573 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatt
//
LOCUS       DACETN010000034        25536 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DACETN010000034 DACETN010000000
VERSION     DACETN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.035"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IXF98_001286"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IXF98_001286"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1555609.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IXF98_001287"
     CDS             complement(937..1518)
                     /locus_tag="IXF98_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1555610.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IXF98_001288"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IXF98_001288"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1555611.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IXF98_001289"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IXF98_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1555612.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IXF98_001290"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IXF98_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1555613.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IXF98_001291"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IXF98_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1555614.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IXF98_001292"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IXF98_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1555615.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IXF98_001293"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IXF98_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1555616.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IXF98_001294"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IXF98_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1555617.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IXF98_001295"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IXF98_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1555618.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IXF98_001296"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IXF98_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1555619.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IXF98_001297"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IXF98_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1555620.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IXF98_001298"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IXF98_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1555621.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IXF98_001299"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IXF98_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1555622.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IXF98_001300"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IXF98_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1555623.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IXF98_001301"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IXF98_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1555624.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IXF98_001302"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IXF98_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1555625.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IXF98_001303"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IXF98_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1555626.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IXF98_001304"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IXF98_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1555627.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IXF98_001305"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IXF98_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1555628.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IXF98_001306"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IXF98_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1555629.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IXF98_001307"
     CDS             13972..14157
                     /locus_tag="IXF98_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1555630.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IXF98_001308"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IXF98_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1555631.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IXF98_001309"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IXF98_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1555632.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IXF98_001310"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IXF98_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1555633.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IXF98_001311"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IXF98_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1555634.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IXF98_001312"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IXF98_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1555635.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IXF98_001313"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IXF98_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1555636.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IXF98_001314"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IXF98_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1555637.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IXF98_001315"
     CDS             18232..18879
                     /locus_tag="IXF98_001315"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1555638.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IXF98_001316"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IXF98_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1555639.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IXF98_001317"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IXF98_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1555640.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IXF98_001318"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IXF98_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1555641.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IXF98_001319"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IXF98_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1555642.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IXF98_001320"
     CDS             20317..21255
                     /locus_tag="IXF98_001320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1555643.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IXF98_001321"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IXF98_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1555644.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IXF98_001322"
     CDS             21935..22774
                     /locus_tag="IXF98_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1555645.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IXF98_001323"
     CDS             22750..23619
                     /locus_tag="IXF98_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1555646.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IXF98_001324"
     CDS             23609..24406
                     /locus_tag="IXF98_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1555647.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IXF98_001325"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IXF98_001325"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1555648.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5497 g         7028 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctc
//
LOCUS       DACETN010000035        25358 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DACETN010000035 DACETN010000000
VERSION     DACETN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.036"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(132..509)
                     /locus_tag="IXF98_001326"
     CDS             complement(132..509)
                     /locus_tag="IXF98_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555649.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="IXF98_001327"
     CDS             765..1493
                     /locus_tag="IXF98_001327"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1555650.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="IXF98_001328"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="IXF98_001328"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1555651.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="IXF98_001329"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="IXF98_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1555652.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="IXF98_001330"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="IXF98_001330"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1555653.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="IXF98_001331"
     CDS             4628..6067
                     /locus_tag="IXF98_001331"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1555654.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6099..7142
                     /locus_tag="IXF98_001332"
     CDS             6099..7142
                     /locus_tag="IXF98_001332"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1555655.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="IXF98_001333"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="IXF98_001333"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1555656.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="IXF98_001334"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="IXF98_001334"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1555657.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="IXF98_001335"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="IXF98_001335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1555658.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="IXF98_001336"
     CDS             10661..11575
                     /locus_tag="IXF98_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1555659.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="IXF98_001337"
     CDS             complement(11708..13273)
                     /locus_tag="IXF98_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1555660.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="IXF98_001338"
     CDS             complement(13266..14717)
                     /locus_tag="IXF98_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1555661.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="IXF98_001339"
     CDS             complement(14731..15747)
                     /locus_tag="IXF98_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555662.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="IXF98_001340"
     CDS             15967..17055
                     /locus_tag="IXF98_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555663.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="IXF98_001341"
     CDS             17109..18635
                     /locus_tag="IXF98_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555664.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="IXF98_001342"
     CDS             18637..19842
                     /locus_tag="IXF98_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555665.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="IXF98_001343"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="IXF98_001343"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1555666.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="IXF98_001344"
     CDS             21340..22764
                     /locus_tag="IXF98_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1555667.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="IXF98_001345"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="IXF98_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1555668.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="IXF98_001346"
     CDS             24337..25314
                     /locus_tag="IXF98_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1555669.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DACETN010000036        25251 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DACETN010000036 DACETN010000000
VERSION     DACETN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.037"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IXF98_001347"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IXF98_001347"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1555670.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IXF98_001348"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IXF98_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1555671.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IXF98_001349"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IXF98_001349"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1555672.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IXF98_001350"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IXF98_001350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1555673.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IXF98_001351"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IXF98_001351"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1555674.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IXF98_001352"
     CDS             5265..5843
                     /locus_tag="IXF98_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1555675.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IXF98_001353"
     CDS             5895..6377
                     /locus_tag="IXF98_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1555676.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IXF98_001354"
     CDS             6494..6868
                     /locus_tag="IXF98_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555677.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IXF98_001355"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IXF98_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1555678.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IXF98_001356"
     CDS             9167..9757
                     /locus_tag="IXF98_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1555679.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IXF98_001357"
     CDS             complement(9885..11234)
                     /locus_tag="IXF98_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1555680.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IXF98_001358"
     CDS             complement(11402..12142)
                     /locus_tag="IXF98_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555681.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IXF98_001359"
     CDS             complement(12155..13747)
                     /locus_tag="IXF98_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1555682.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IXF98_001360"
     CDS             complement(14315..14611)
                     /locus_tag="IXF98_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555683.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IXF98_001361"
     CDS             14787..15512
                     /locus_tag="IXF98_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1555684.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IXF98_001362"
     CDS             15505..16347
                     /locus_tag="IXF98_001362"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1555685.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IXF98_001363"
     CDS             16350..16871
                     /locus_tag="IXF98_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1555686.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IXF98_001364"
     CDS             complement(17124..17687)
                     /locus_tag="IXF98_001364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1555687.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IXF98_001365"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IXF98_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1555688.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IXF98_001366"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IXF98_001366"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1555689.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            20907..21173
                     /locus_tag="IXF98_001367"
     CDS             20907..21173
                     /locus_tag="IXF98_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1555690.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21317..23308
                     /locus_tag="IXF98_001368"
     CDS             21317..23308
                     /locus_tag="IXF98_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1555691.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23662..24963
                     /locus_tag="IXF98_001369"
     CDS             23662..24963
                     /locus_tag="IXF98_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1555692.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8136 a         4782 c         5182 g         7151 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaatcag ctgtccctcg atattagctc aaaaaacggc aaaatatgag gaactatttt
    20701 tccgtttgtt tctctaatac tcaggtcaaa ccgctgattt cacaacctct gattcacggt
    20761 gttccagaag cagcttctac ggaaataagc tgaacacttt tgttacaact tctttttttc
    20821 tggagtttct tggagcatat ttttggagtc cttctgaata aagcgtaaaa taaaagtgag
    20881 aaatatggat aaaggaggga aaggagatgc tgaatttttt atggtcgtta tttatcggcg
    20941 gtatattagg tttgatagct ggcgcaattt taggaaagga agtcccggga ggcgtatctg
    21001 gaaatatcat tatcggtttt ttaggtagtt ggctcggcga atttttacta ggaccactgg
    21061 gaccggaact cggcggtttt tatttgatac cagcattatt gggtgcaatt ttttgccttg
    21121 ctctttattc atttgtggca agacgtatca gagaacgtcc aaagaaagaa taattttatt
    21181 caaataagtt gacacttcag tgtgttactt ggataattag aagcaattta ggaaagcggg
    21241 tgtcatggat aagaatatca tctatcattc aagcttacct aaaggatatc catataacaa
    21301 gaaatgaggg aaaaacgtgg caacaacgca caataccaaa aagatatcaa tggctggcct
    21361 gcttgtagcg atgggagtcg tctacggaga catcgggaca agtccgcttt acgttatgaa
    21421 atcgattgtt gaagataatg gcggattgtc aggaatcaat cctgattttg ttatcggatc
    21481 agtctcattg attttttgga cattgactct actgacaaca atcaaatatg tcgtgattgc
    21541 attgaatgca gataaccatg gtgaaggtgg aattttctca ctctacacac ttgtccgaaa
    21601 aggtgggaaa tatttgatca ttcctgcaat gatcggagga gctgccttac tagcagatgg
    21661 ggtattgacc cctgctgtga cggtaacgac tgcgattgaa ggcttgcgag gaattccgca
    21721 gttttttgac cggttcggta ataaccagaa cgtgatcgtc attatcactt tagttattat
    21781 ttttgtttta ttcatggtcc aacgctttgg tacagatatt gtcggaaaag cattcggacc
    21841 aatcatgttc gcttggttta cttttttagg cgtaatggga ttaatgaact tcagtcaaga
    21901 ctggacagtt atccgtgcac tgaatcctta ttatgctatc cagctgcttt ttagtgcaga
    21961 taacaaatta ggcttgttca ttcttggaaa tattttcctg gctacgacag gtgcagaggc
    22021 gctttattcc gatcttggac atgtcggcaa acgaaatatt cgtctgagct ggccttacgt
    22081 caaaatttgt ttggttatca attattttgg gcaggctgcg tggttgttga atgtttatca
    22141 aaaccctgaa gcacagcaaa tcaagaacct gaatccattc ttccaaatga tgcctcaaag
    22201 ttggatcgtg atcggcgttg cttttgcaac agtcgctgca gtcattgctt cgcaagcatt
    22261 gattacaggt tcgtttacgc tagtttcaga agcaatcaag ttgaaactgc tgcctcggtt
    22321 aaagatcatg tatcctggga acagtatcgg gcaaatgtat atcccagcag tcaatctgat
    22381 tttatggatc gcctgttctc tagtggttgt cacattccga acgtctcatc atatggaagc
    22441 tgcttacggt ctatcgatca ctgtgacgat gttgatgaca acagcactgc tttactttta
    22501 tcttatccaa aatgggcatt ctcgttggct tgcatattta gttacctttt tcttcggtgc
    22561 gatagaagtc gttttcttta tctcaagtgt tgtcaaattc ttccgaggcg gttttgtagc
    22621 cgttctgatt ggtttgatca tcttagtcgt gatgtttatt tgggaacaag ggaacattat
    22681 tcgcgaatcg tcaaccgagg atgtgtcttt gagagattat atcccgcagc ttagagaatt
    22741 acgtgatgat acttctttac caatctatca gacaaatgtg gtcttcttgg ttcctgatat
    22801 ggctgaagat aaagttggcc gacagttcat ctattcgatt ttagataaac ggcctaaaag
    22861 agcacgagtc tattggtttg ttcatgtgga agtgacggat gagccatata cgaaagaata
    22921 tcaagtagat atgatggaca ctgattttat tgtccaagtg aatctgttct taggcttcag
    22981 ggtccaacag gaaatcaacg tatatgttcg ccaagtcatc catgatttga tgaaacaagg
    23041 ccgtttgcca aaacagccac aaagatactc tctgacacca gggcgagaag ttggcgattt
    23101 ccaatttgtc ctgattgatg aagtagtttc caatgtgaca actctaggaa aatggcaacg
    23161 tcaaatcatg caggcaaaac tggcaatcaa aaaaatcgct acctcgccag aaacatggtt
    23221 tgggctagag tacagcgaag taaaacatga aactgttccg cttatcattg gaaatactcg
    23281 gaaaacatgg ttgaaagagc ggttgtgaaa gtagcgcttt gacctgaact aatgtcgaat
    23341 gtcagctact tgaacaccgt ttatcagagg tcatgaaaaa gctgtactgc aacaaacagt
    23401 aagaacgagc agataaaaac agcttctcat gtttttcatc tgtttgggct taatgccgca
    23461 gatgcaagct tttgaatacc gtttttatca gaggtcatga aaaagctgtt tagcaacaag
    23521 taacgcaatg attttgaagt gataaggaga tgtggctgaa aaatgccgca tctccttttt
    23581 tattcctaaa tatgaatacg cattattttc aatctttttg tagaaaaagg gtacaataga
    23641 aaaagagaaa ggcgggagaa tatggttatc aatttcatta ttttattttt gatgattgca
    23701 attacttcat tttttgtagc aagtgaattt gctttagtga aaatcagacg atcaagattg
    23761 gaacaactag aaaaggaaaa tgtaaagaac gccaagctag ctcttcatgt tacacaccat
    23821 ttagataact atttatcagc tagtcaatta ggaattacgc taaccggttt gatcatcggg
    23881 tgggtcggtg aaggatctgt cgcggcttta ctagaaccag tgatcggtaa actacctttt
    23941 agttcggcta ttagcagtac aatctctgtg gcgctgggat ttattttagt tacgtatatc
    24001 gatgtggtta ttggcgaact gcttcctaag agctatagta tcgtcaatac ggagaaggtc
    24061 gtcttgttcg tagttaaacc acttcattat ttttataaag taatgtttcc ttttatttgg
    24121 gtattgaatc attcagctgc agggctaggg aagctattag gtgtgcgtct cgtatctgaa
    24181 ggagaagaga cattgtctca agaagagctg acattagtcg cattgaattc ttatgaaaaa
    24241 ggagagttga ccaaagaaga atatcattat ttggaaaacg tgtttgaatt tgatgatacc
    24301 ttggcaaaag atatacaagt agaccgtacg tccatgcaag tattcgaagc ttcagaaacc
    24361 gtcaaagaag cgataaaagc ttcgattgaa caaggacata ctcgctatcc ggttatctta
    24421 gaatcaaaag acaatgtttt aggttatgtc accttgccag atttgatcaa gcaatcttat
    24481 aaagatgacc agctgacagt agaacagttg attgaagagc cgatcgtaac aactgaaacg
    24541 atccctataa aaaaattatt gacgatcatg cgaaaaaagg gaaagcatat tgctatctta
    24601 aaagatgaat acggagggac aagcggctta gtgacgattg aagatatttt agaagaaatc
    24661 gtcggggaaa tccgagatga gacagatttg gacgaagcat tgatagcgga acagtcagat
    24721 ggctcttata ttatctccgg caaactaaca ttagatgatt tccagcgtta ttttcatgta
    24781 gagattccag agtttgaaga aacgaatttt actacacttg cgggctttgc ttctagccga
    24841 tataaagaaa taaaagcagg aacaatcatt gagattgcat cattccgttt cacggtatta
    24901 gaataccagc atgcacatat cgattatttc aaagtagagt ccacggaaag aaaaacagaa
    24961 taaaagaaaa aaggctgtga cattttttgt cacagccttt ttcctacgat tagtcaagta
    25021 aaaggtgcaa gaaaaatagc tcgtaaacga aagcgacaaa atcaaagcac accaaataaa
    25081 gccccgttcg taaaaaattt tcgatctggg caaagaagtg gtatactgta agtggtttag
    25141 ttaatggtcc actgagaccg cgtacgcata gtcgtactct gtatgtgccc taaccatagc
    25201 gtgcatacat ttcagaagct tgttcataca agcaactgtg gcaaccttat c
//
LOCUS       DACETN010000037        25235 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DACETN010000037 DACETN010000000
VERSION     DACETN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.038"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            5..403
                     /locus_tag="IXF98_001370"
     CDS             5..403
                     /locus_tag="IXF98_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1555693.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IXF98_001371"
     CDS             387..1082
                     /locus_tag="IXF98_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1555694.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="IXF98_001372"
     CDS             complement(1676..2563)
                     /locus_tag="IXF98_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1555695.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="IXF98_001373"
     CDS             2748..3587
                     /locus_tag="IXF98_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1555696.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="IXF98_001374"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="IXF98_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1555697.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="IXF98_001375"
     CDS             5055..5411
                     /locus_tag="IXF98_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555698.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="IXF98_001376"
     CDS             5414..6571
                     /locus_tag="IXF98_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555699.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="IXF98_001377"
     CDS             complement(6597..7037)
                     /locus_tag="IXF98_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1555700.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="IXF98_001378"
     CDS             7197..7703
                     /locus_tag="IXF98_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1555701.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="IXF98_001379"
     CDS             7871..9784
                     /locus_tag="IXF98_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1555702.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="IXF98_001380"
     CDS             9789..10790
                     /locus_tag="IXF98_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1555703.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="IXF98_001381"
     CDS             complement(10992..11198)
                     /locus_tag="IXF98_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1555704.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="IXF98_001382"
     CDS             complement(11844..12692)
                     /locus_tag="IXF98_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1555705.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="IXF98_001383"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="IXF98_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1555706.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="IXF98_001384"
     CDS             13760..14761
                     /locus_tag="IXF98_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1555707.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="IXF98_001385"
     CDS             14758..15042
                     /locus_tag="IXF98_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1555708.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="IXF98_001386"
     CDS             15075..15872
                     /locus_tag="IXF98_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1555709.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="IXF98_001387"
     CDS             15886..16719
                     /locus_tag="IXF98_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1555710.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="IXF98_001388"
     CDS             16737..17594
                     /locus_tag="IXF98_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1555711.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="IXF98_001389"
     CDS             17616..18125
                     /locus_tag="IXF98_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1555712.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="IXF98_001390"
     CDS             18162..18563
                     /locus_tag="IXF98_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555713.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="IXF98_001391"
     CDS             18675..19028
                     /locus_tag="IXF98_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555714.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="IXF98_001392"
     CDS             19062..19505
                     /locus_tag="IXF98_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1555715.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="IXF98_001393"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="IXF98_001393"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1555716.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="IXF98_001394"
     CDS             20600..21313
                     /locus_tag="IXF98_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1555717.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22065..22265
                     /locus_tag="IXF98_001395"
     CDS             22065..22265
                     /locus_tag="IXF98_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1555718.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            22833..23396
                     /locus_tag="IXF98_001396"
     CDS             22833..23396
                     /locus_tag="IXF98_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555719.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(23457..23822)
                     /locus_tag="IXF98_001397"
     CDS             complement(23457..23822)
                     /locus_tag="IXF98_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555720.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(23898..24143)
                     /locus_tag="IXF98_001398"
     CDS             complement(23898..24143)
                     /locus_tag="IXF98_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1555721.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(24213..24467)
                     /locus_tag="IXF98_001399"
     CDS             complement(24213..24467)
                     /locus_tag="IXF98_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555722.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(24631..24954)
                     /locus_tag="IXF98_001400"
     CDS             complement(24631..24954)
                     /locus_tag="IXF98_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555723.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            25141..25214
                     /locus_tag="IXF98_001401"
     tRNA            25141..25214
                     /locus_tag="IXF98_001401"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25175..25177,aa:Ile,seq:cat)
BASE COUNT         8770 a         3881 c         5143 g         7441 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcttgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct ttgttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgcgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta atgcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa
    22321 aaaattcatt ggtcgttgaa tgggctgaat agtagtgtat aaaacaattt tgtggagcgt
    22381 gaagctttta tggtagagga aaatacgaat aaagtgatgt tgataaaaag attcctatct
    22441 tgaatatgaa tcgagatgtc ttgaaaatga atgagtttga ccaatcatta tcctatcgaa
    22501 atttacagtt tcgttcgatt ggtctgtcaa ttaatgaaaa agtaaaaaag aatatgttct
    22561 aggaagaggg cggcacatct aaatttttaa gtaacggaca gttcaaatat caagcattga
    22621 aagaggctat cctactgttt gaaggaatac gaatatataa aaacgacaaa gaggaaaaag
    22681 tagttcatta aatcccttca agtgatgaaa gaatgagatc taataaaacc tgatctttca
    22741 tctgtttttt taataacgta ataatttttt tattttttgt gctttcgccg ttattgtaga
    22801 gtgatttagc attcttattt ttaaaaataa ctgtgttata tattaaaaaa ggcataatag
    22861 ttataagctt ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag
    22921 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
    22981 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
    23041 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
    23101 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
    23161 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
    23221 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
    23281 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
    23341 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
    23401 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
    23461 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
    23521 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
    23581 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
    23641 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
    23701 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
    23761 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
    23821 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
    23881 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
    23941 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
    24001 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
    24061 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
    24121 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
    24181 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
    24241 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
    24301 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
    24361 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
    24421 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
    24481 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
    24541 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
    24601 gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc
    24661 taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat
    24721 gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct
    24781 ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat
    24841 cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc
    24901 cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa
    24961 taaataaaag aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag
    25021 ttatgacaca agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt
    25081 gtgagagttt gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat
    25141 gtacctttag ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag
    25201 tcctacaagg tacataagag gttgtgaaaa agctg
//
LOCUS       DACETN010000038        24860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DACETN010000038 DACETN010000000
VERSION     DACETN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.039"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            129..1388
                     /locus_tag="IXF98_001402"
     CDS             129..1388
                     /locus_tag="IXF98_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1555724.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1477..2424)
                     /locus_tag="IXF98_001403"
     CDS             complement(1477..2424)
                     /locus_tag="IXF98_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1555725.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2818..4110
                     /locus_tag="IXF98_001404"
     CDS             2818..4110
                     /locus_tag="IXF98_001404"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1555726.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4125..5894
                     /gene="aspS"
                     /locus_tag="IXF98_001405"
     CDS             4125..5894
                     /gene="aspS"
                     /locus_tag="IXF98_001405"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1555727.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            6003..6878
                     /locus_tag="IXF98_001406"
     CDS             6003..6878
                     /locus_tag="IXF98_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1555728.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7144..9027
                     /locus_tag="IXF98_001407"
     CDS             7144..9027
                     /locus_tag="IXF98_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1555729.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9102..9386)
                     /locus_tag="IXF98_001408"
     CDS             complement(9102..9386)
                     /locus_tag="IXF98_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1555730.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9376..10110)
                     /locus_tag="IXF98_001409"
     CDS             complement(9376..10110)
                     /locus_tag="IXF98_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1555731.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10239..10862
                     /locus_tag="IXF98_001410"
     CDS             10239..10862
                     /locus_tag="IXF98_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1555732.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11054..12175
                     /locus_tag="IXF98_001411"
     CDS             11054..12175
                     /locus_tag="IXF98_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1555733.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12182..15310
                     /locus_tag="IXF98_001412"
     CDS             12182..15310
                     /locus_tag="IXF98_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1555734.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15329..18523
                     /locus_tag="IXF98_001413"
     CDS             15329..18523
                     /locus_tag="IXF98_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1555735.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18669..19376)
                     /locus_tag="IXF98_001414"
     CDS             complement(18669..19376)
                     /locus_tag="IXF98_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1555736.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            20141..20539
                     /gene="spxA"
                     /locus_tag="IXF98_001415"
     CDS             20141..20539
                     /gene="spxA"
                     /locus_tag="IXF98_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1555737.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            20871..21524
                     /locus_tag="IXF98_001416"
     CDS             20871..21524
                     /locus_tag="IXF98_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1555738.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            21750..22748
                     /locus_tag="IXF98_001417"
     CDS             21750..22748
                     /locus_tag="IXF98_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1555739.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            22924..24732
                     /gene="pepF"
                     /locus_tag="IXF98_001418"
     CDS             22924..24732
                     /gene="pepF"
                     /locus_tag="IXF98_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1555740.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         8355 a         4259 c         5212 g         7034 t
ORIGIN      
        1 ttaagttaga ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt
       61 ttgtcagcag tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt
      121 tgctgaagat gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt
      181 atcaaaaagg atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca
      241 aagcaaagat tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat
      301 tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac
      361 tttttggtca aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata
      421 tttttatgga tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga
      481 atctgacaca agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga
      541 tggttttctt tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag
      601 atgaggtcga aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc
      661 cgattgatca cataaaaggt gtcgaaaatc gcaaaaaatc agaaaaatgg gcgcaaggct
      721 tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt
      781 ttgccaatgc cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat
      841 tattgaacat catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg
      901 cacttttcag tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag
      961 atccgtttat tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt
     1021 cgctgaaaga agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag
     1081 atttatatgg aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca
     1141 aacggtatgt agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac
     1201 aaggcggggg aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata
     1261 gtctacgtgt gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag
     1321 attggacgat tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca
     1381 agaattagat tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct
     1441 tttttcatat tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc
     1501 aatggtttga taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac
     1561 ttgctctgga cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca
     1621 agctaaaaca agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc
     1681 gacagcggtt aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg
     1741 atctccttca ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga
     1801 agcaagagga aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc
     1861 tttttcagcc tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc
     1921 tgcattcgct tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt
     1981 tcctgcttct tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc
     2041 cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc
     2101 caccgattgt ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg
     2161 agcagctttt gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc
     2221 gccaatcagt tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat
     2281 cccgctattc aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa
     2341 aagattatgt cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga
     2401 atgtccgatt cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa
     2461 taccaaaaag ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt
     2521 tgagactggt ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt
     2581 ttttgaaact ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc
     2641 taaagacact ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa
     2701 gaggaaagag aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt
     2761 tcatgatgaa caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg
     2821 agtttccaaa gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag
     2881 tttgtcgaag aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca
     2941 ccgatttttg aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt
     3001 tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa
     3061 ggtacagcac caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg
     3121 aaaccgttca aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga
     3181 cggttacgtc aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact
     3241 gatgtggaga gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc
     3301 cggctagtca tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg
     3361 atcgactatc ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat
     3421 gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa
     3481 gctccatcta tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt
     3541 tccatgctgg atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta
     3601 gattattaca cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa
     3661 gcaacgattt gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca
     3721 acacctggct tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag
     3781 gaagtagtta tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa
     3841 acaaatatcg aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac
     3901 cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc
     3961 aaattagtat tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca
     4021 atggcaagcc gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa
     4081 gtctatgatg aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga
     4141 tctattgcgg attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg
     4201 tacaaaaaag acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa
     4261 tcgttcaaat cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat
     4321 gtcgcagtga atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag
     4381 taaatcctaa aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga
     4441 atcaagcaaa aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac
     4501 gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa
     4561 gaaatgcgac aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg
     4621 aaacacctta cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac
     4681 gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc
     4741 tgatgaatgc tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt
     4801 tacgtggaga ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag
     4861 cagaagaaat ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg
     4921 gtatcgaagt cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg
     4981 gcagcgacaa accggataca cgttttgata tggaattgat cgatctaagc gatacagtca
     5041 aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat
     5101 tgaatgctaa aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat
     5161 atgtaggaca attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga
     5221 aaggtccaat tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg
     5281 caaaaccagg tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat
     5341 taggtgctat ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca
     5401 atttcttatg ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg
     5461 tctctgctca ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag
     5521 accctgcaaa agtctatgca gaagcttatg atatcgtctt gaacggttat gaactaggcg
     5581 gaggttctat ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct
     5641 ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat
     5701 tccctccaca tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag
     5761 aagataatat tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga
     5821 gtgatgcacc aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga
     5881 atatagaaga atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca
     5941 accttgattt aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt
     6001 ccatgaaaaa aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat
     6061 ctatcgttta tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata
     6121 tttattcaag tggtattact ggtctagctc agattttgac gactttgtca aaatcactga
     6181 taggattcga agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat
     6241 tggcgtggtt aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga
     6301 cctccctttg tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg
     6361 cgttgtttgg gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat
     6421 catcgggcgg tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg
     6481 gttctatttc gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct
     6541 ggcaatatat gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg
     6601 tttatactaa acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag
     6661 taaatgaaat ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg
     6721 cttataagca tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc
     6781 tacttagaga agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg
     6841 tccagatact tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat
     6901 gaaatactgc tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc
     6961 agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta
     7021 tttttttaaa tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg
     7081 ctattttcac ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat
     7141 tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc
     7201 cgttcattag ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc
     7261 ggctatttgg gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg
     7321 cggcaggaat gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg
     7381 aagagactct atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg
     7441 tttcagcgag ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc
     7501 aacaactggt ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca
     7561 ccgtttacgc atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc
     7621 ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta
     7681 ggtacatcct tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca
     7741 acccgtgttt attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt
     7801 gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt
     7861 ctgaatatcg ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat
     7921 gatcattaca atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg
     7981 ctagttgcag tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta
     8041 tttacttgga ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt
     8101 ttttatgaag caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa
     8161 atcacattga ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca
     8221 cgttttgatt atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc
     8281 agtaagagca aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt
     8341 ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc
     8401 gctgaaagta aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg
     8461 gttgaccaag cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa
     8521 aaattagaac taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa
     8581 gaactttatc caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta
     8641 ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag acactgttct gatcggacta
     8701 ggaacgaatg gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat
     8761 cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat
     8821 gcgatgcttg aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc
     8881 tacagtaatg accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg
     8941 atgacacatt acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac
     9001 agcagtgaga caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt
     9061 ttttgcctga agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc
     9121 aaaacggttg agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat
     9181 gcagcttctg cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta
     9241 acaggtcttc ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct
     9301 aatcgtctag aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat
     9361 acgtaaaaat aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc
     9421 atccatattg taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt
     9481 gattccttca actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat
     9541 ccttttaggt gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg
     9601 acgatggacc atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac
     9661 ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt
     9721 tttatttgtt ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt
     9781 atgtcgaacg acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt
     9841 taaatggtta agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat
     9901 agcgtcgata gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc
     9961 acaaaggtca acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc
    10021 atctaaagaa aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa
    10081 ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg
    10141 tctgtattat actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt
    10201 atgcatacta tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg
    10261 gggtagttcg agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag
    10321 ttttaccgca agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat
    10381 tatatctggc ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt
    10441 tcaaaaatcc agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag
    10501 aaaatccagg accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga
    10561 gtttgatcat gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat
    10621 tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac
    10681 ttggggatct gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat
    10741 cagatatcaa gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag
    10801 cttttgacaa attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat
    10861 aaaatgataa gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact
    10921 ttttcaatga aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta
    10981 aaatagaaga caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt
    11041 ttgaggtgat tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca
    11101 aggttatgac ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa
    11161 agaagaaaaa gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc
    11221 tgttgatgcc gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat
    11281 cccgattttt gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc
    11341 atggttcagt tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt
    11401 cgagtttaaa aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt
    11461 atattttgaa gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa
    11521 aatgcaggag ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat
    11581 gggaagtgta aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc
    11641 tggtgaacta cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc
    11701 tttgagacag gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat
    11761 caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag
    11821 ggagatcgaa cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga
    11881 tcctgccttt tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag
    11941 aacgatcatc ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat
    12001 tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa
    12061 agatcctcaa aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc
    12121 aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag
    12181 aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga
    12241 tttttcacgt ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa
    12301 aacgacgatt tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg
    12361 ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt
    12421 ttcttttgaa catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc
    12481 aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga
    12541 tgggaaagga aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag
    12601 agaactgatg aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga
    12661 attccgtaat tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt
    12721 cggtactcaa tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca
    12781 acaaaaattg gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt
    12841 caaagaagag gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca
    12901 ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa
    12961 acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga
    13021 aaaaacgata ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga
    13081 aaagaaacga tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg
    13141 cctacaagaa ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa
    13201 gaaactagcg atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt
    13261 acgccgacaa acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt
    13321 gccaattgtc gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt
    13381 ggataaagaa gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag
    13441 aactcgaatt cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc
    13501 ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa
    13561 agacactggg tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac
    13621 aaaaaatagt cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg
    13681 ggcaaagcag caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt
    13741 ttgtggatca acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga
    13801 tatcttaaaa gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca
    13861 aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga
    13921 acaaaaaatc atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc
    13981 tttgaaaaag gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat
    14041 cgaacaatct ttagatagac gaaaaagcaa gatggccaat gcaagaatag agctggaaga
    14101 aataagacaa caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac
    14161 agaaaaaaat aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt
    14221 gcaagatacg gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga
    14281 agaagactcg aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct
    14341 tcatacgctt cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa
    14401 aatagaaaag gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac
    14461 aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac
    14521 agttacttct tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa
    14581 tatccagcta actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat
    14641 aagacgtatg atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag
    14701 taatcaaaaa atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga
    14761 tgagcttacg caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac
    14821 tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga
    14881 tcatctccaa cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag
    14941 ttatcgaggg aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg
    15001 aagtgcgcat accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc
    15061 tcttgccgat gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga
    15121 tgaaggattt gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac
    15181 gatcgaaagt gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg
    15241 agtattacaa caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca
    15301 acttggataa aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc
    15361 acgtggacgt caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga
    15421 aaacgtgtgg catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac
    15481 agcaaaagag atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca
    15541 tacagttgct gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga
    15601 ccagccaatg ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt
    15661 ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca
    15721 agtagaaacg attgagacta atcagtatca cccagagctt tctattttgg cactttactt
    15781 gcgtatcggt tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt
    15841 tttacaagct tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct
    15901 agataaacgg gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc
    15961 tcagacgcat caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag
    16021 atatctgctt ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac
    16081 acgtttgcat ctgcagataa atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa
    16141 caaaccttta tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa
    16201 tgattttgat tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc
    16261 tctgacgaaa tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga
    16321 gaaaccagaa gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc
    16381 tctgctgtca gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga
    16441 tatccccgtc gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt
    16501 cgatttcgtc tatggcgaag tcatctattc gactgacccg aagcatgagg tctcgacaga
    16561 acaggctttt caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag
    16621 aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga
    16681 actatatgcc tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact
    16741 tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt
    16801 aaccgagacc ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga
    16861 aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg
    16921 tcaggttctt tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt
    16981 acgggaatcg atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat
    17041 cattcaaaat caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt
    17101 acaagatctc attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac
    17161 gatgagagat tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt
    17221 tggcggtatt ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt
    17281 actttcggaa aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt
    17341 aatttacaac tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat
    17401 caatgggaat aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac
    17461 ttcttatgcc agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt
    17521 gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag
    17581 agaattagct gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga
    17641 agaattgtgg tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt
    17701 cagggaactt gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga
    17761 taaacaaact gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact
    17821 tacagaagaa caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac
    17881 atcaatggat tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag
    17941 actgcgacaa atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg
    18001 caagctcgaa caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt
    18061 gctgttttct caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg
    18121 gatctcgaca ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga
    18181 cgcatttaat gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg
    18241 attgaatctc acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat
    18301 tgaagaacaa gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg
    18361 gcgtatgatc tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg
    18421 cgaactgttc caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga
    18481 agacatccgc atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt
    18541 tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag
    18601 caaggaggct gggattccat tttatcccag cttccttgct atcatttttt tatttctatt
    18661 tttaaaggtt atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc
    18721 tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct
    18781 tgccagttag cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag
    18841 gtctcttctg gaagcatatg aaattctttc tcagctagct gaaaaatctc tttttctggt
    18901 ttttggaaac ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat
    18961 tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat
    19021 gggacattct tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata
    19081 ttatcaagtt cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga
    19141 gggtagtcta aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc
    19201 caatctcctg ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat
    19261 agtggatgca tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat
    19321 ggctgctgct gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt
    19381 ttacacgcct ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa
    19441 aattgtctaa aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca
    19501 gaataaggtt tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt
    19561 tgatgaacaa attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt
    19621 ggaattttcg aaagatttat ttttttaata cagtctcaat ataactattc aaacataaaa
    19681 taacgttatt tgatttattt gtttatagta aattttattt agtaatcatt gctttaaaat
    19741 actacgctta tataacagag aaaataagcg ttataataaa gaacagtatt atttaaaatg
    19801 acagaaaaca ttactattaa agaaataaaa aacataaaaa atcttaagaa atcggtttca
    19861 tttagctttt gagataaaat aatagtacag ttttatacgc tgtttcatat gtttagaaaa
    19921 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
    19981 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
    20041 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
    20101 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
    20161 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
    20221 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
    20281 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
    20341 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
    20401 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
    20461 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
    20521 attgatggct ggattataga aggataatac gaaacttgga aaagactatt acaaagatag
    20581 tttttccaag tttttttgtt tttacaaaaa gtattcttga ttagatgtat aaaaaggtag
    20641 tagaagatga tgattgcacg aaaaatagag agggaaaatt agctgaaaag tcggctatat
    20701 agtattcgtc tgccaaaaat gttatagtta aataagatag cttgaaaggt tgaaaaatcc
    20761 gctggtttta ttaaattcgc ttaacagcga gatttttttt ctttacattt aagctgattt
    20821 cgctacaatg aatctatatt aaacattgtg gcaaagtgag gtgtagcaaa atggaaatgg
    20881 aacatattaa tgaaaatacg atccgtgtac taattggtca tgaagattta gaggaacggg
    20941 gcgtttcatt tttggattta cttggcaatc acaaagaaat cgaaaatttc ttttatagta
    21001 ttttggaaga agttgatatc gaagaggagt tccgaggaag cgaagctgtc actttccaag
    21061 ttttgccaaa aggagacgga ttagaactgt ttatcagtaa aaatcttccg gatgaagaaa
    21121 tttctcaatc agaagattct actgttgatt cagaagatgt gagtgacttt cttcgtcagc
    21181 agattattgg cagcgatccg gaggaagaat tatctggaag cgccctagaa gaacaggaaa
    21241 gacaagcaac ttttgctttt gaagattttg aacaagtgat ccaacttgca acagatgtcg
    21301 aactagagtc tgcttggact gatttgtatt tattcgaaga gcgctattac ctagtcgttc
    21361 acttctggat ggaaaatctc aaccaagcgg atgtagagaa tcagattgct cgaattttgg
    21421 agtttgctga aaagagcgat cgtacaccag aaacattgaa tgaatatgga caatgtctga
    21481 tggcgcgtaa tgctatcgag cgaacgaaat tttattttgt ataaatcagc attatagtaa
    21541 gagactgtga caaaaattgt gtcgcagtct ttttttcgta taaatgtgtt caaaagcttg
    21601 catctgcggt aacaagccca accaaacgaa aaatattggg aactattttt gtttagctgt
    21661 tcttattact tgatgcagaa tagctttttc acgccctctt ttttcgtgta aataaattct
    21721 tagacgtaaa taagttcaga gggagaaaaa tgctgcttgc aatgaatgaa tataataagt
    21781 gctgttttgc taaacatgca gaaaaaggaa agaaatatta ttgtcgtggc tgtcgaaaaa
    21841 gagtgatttt gaaaaaggga aagaaaaaat gtgctcattt tgcccatcaa aaatcagata
    21901 actgttcggt ctttagtgaa agagaatcaa aagagcactt acagttaaag gaatgcttca
    21961 tggattggct gggacagtca gtagaaccag tatttttgga agcctatttg ccgaggttgc
    22021 ggcaaaggcc ggatattttg ttggcgaatc tagcaataga aatccaatgt tctagactgt
    22081 ctcaccagag atttattgaa agaacccaaa attatttgaa taacagttac caagtatggt
    22141 ggatacttgg taattctttt cttggacaat cgcaattctc attgatagaa aaaagttgct
    22201 gctactataa tagaaaaaga ggagtgcatt gctggaaagc tgatctcaag aaacaaaagc
    22261 tttatttgta tcatcatatt acagagactg tatcagggca tatttccttt tgcagtagct
    22321 gctggacttt ttctagtagg gaccttaaag aaatattcac tagtaacgaa attaaaatga
    22381 accaaatgaa aaaaatagaa aaagtgagtg aagatgggaa gaaatggctg acacgccaac
    22441 ttattcacaa gcaaaagaat acagtatcca tacaagaaca atgctacttg cgtcataaac
    22501 atcttttgca tctatcatcg tggatctatc aaaaaagtcg gtttttcttt tatttacaag
    22561 aacaggtttt tctttatcga atgttgtacg aagaagcctt ggaaaaccag aaagttcctg
    22621 atttcaactc atggctttgc caaataaaag aacataaaag ggaatggctt tttcctatga
    22681 ttgatgaaga gatggtctat cagcagtttt ttaacgaatg catacacttg tcttctttga
    22741 aaaaatagta agtataaaaa taggctagga atcggttttc tatgtaaaac aagatttagt
    22801 tatgctaaaa ttgatggaag cagacacatg aatgagtaga aaagttagat gctgtgacat
    22861 gtggatgtta gactatgatc tattttgata gatagagcta agggaagaaa aggagtggat
    22921 ttcatggaag taaagcagtt gccaaaacga gaagaattgc ctgaaaattt aacttgggac
    22981 ttgaccaaga tcttttcaag cgaccaagag tttgatgaga aatatttgga attatcagaa
    23041 gagttaaaac aatctgaaaa acacaaagga acacttgatc aaggcgcttc tcaattttta
    23101 aatgcgattg aattcgtatt gagggtttat cgccaaactg aagtcattta tgtatatgcg
    23161 caccttaaaa acgatcaaga cactggaaat acagattacc aagcgcttta tgcaagagca
    23221 agcagtctgt tttcgaaagt tagtgaagcc gtttcctggt ttgaaccaga aatattgcaa
    23281 ttgtcagatg accagatttg gcaatatttc aaagaagaac caaaattgga agtctatcgc
    23341 cattatatcc agcaaatagt agataatcga gcccatgtct tatctgctga gcaggaatct
    23401 cttcttgctg gagcaggtga aatctttgat gcttcaagtg atacatttgc tgttttgaat
    23461 aatgcagatc tagtttttcc aacgattgaa ggagaaaatg gtgaaatagt ccaattatct
    23521 catggcgtgt atggtcagtt gctagaaagc acggatcgaa gggtgcgcga agcagcattt
    23581 aaggggttgt acagtgttta cgaacaattt agaaatacat ttgcttctac tttaggcaca
    23641 catataaaag gacataattt taaagcgaaa gtccgtaatt acagctctgc cagagaagcg
    23701 tctttgagca ataatcatat tcctgaaagt gtatacgata ctttggtaga cgtggtaaac
    23761 aagcatttgc ctttgttaca tcgatacatg gaattacgga aacgtttatt agaagtggaa
    23821 aaactgcaca tgtatgatct ttatacaccg gtcttagggg aagctccaat tacctttacg
    23881 tacgaagaag caaaagaaaa agctttagaa gcactgaaac caatgggtga agaatacatg
    23941 gccatcgtag aaaaagcatt ctctgaacgt tggatcgatg ttgtcgaaaa taaagggaaa
    24001 cgaagcggtg cttattcttc gggaagctat gacacaaatc catatatttt attgaattgg
    24061 catgatacgc tggatcagct atttacgctt gtccacgaaa tgggacatag tgttcatagt
    24121 tatttcactc gttcgaacca gccttatgtg tacggcgact actccatctt tttagcagaa
    24181 attgcttcga caacgaatga aaatatccta acggagtatt tattggaaac agaaaaagac
    24241 cctcgtgtac gggcttatgt actcaaccat tacttggatg ggtttaaagg aacagttttc
    24301 cgccagacac aattcgctga attcgagcat ttcatgcata cggaagatga aaaaggtgtg
    24361 ccattgacta gtgaatacct aagtgatagt tatggtaaat tgaatgcaaa atattatggt
    24421 ccagcagtcg aagaagaccc agaaattaaa ttcgaatggt cacggatacc gcatttttat
    24481 tataattact atgttttcca atactcgact ggcttttctg ctgcttcagc actggcgaag
    24541 aaaatactaa accaggaacc agaggcacta gagaactact tggcttacct aaaagcaggt
    24601 aacagcgatt atcctgtgga agtaatgaaa aaagcaggag ttgatatgac acaggctgca
    24661 tatattgaag atgcaatgtc gatgtttgaa caacgtctaa atgaattaga agaattgatc
    24721 gatcgtttat aaggctttct atttttgatt ttctcgaata gaaacgaaaa aaatacgtcg
    24781 gctctcttgt aagggagtcg acgtattttt ttaaggctct atactttttg atgttggtga
    24841 acttcaccaa catctttttt
//
LOCUS       DACETN010000039        24665 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DACETN010000039 DACETN010000000
VERSION     DACETN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.040"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            236..1042
                     /locus_tag="IXF98_001419"
     CDS             236..1042
                     /locus_tag="IXF98_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555741.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="IXF98_001420"
     CDS             1054..2274
                     /locus_tag="IXF98_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555742.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="IXF98_001421"
     CDS             2264..3850
                     /locus_tag="IXF98_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1555743.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="IXF98_001422"
     CDS             3889..6027
                     /locus_tag="IXF98_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1555744.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="IXF98_001423"
     CDS             6315..7706
                     /locus_tag="IXF98_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1555745.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="IXF98_001424"
     CDS             7765..8574
                     /locus_tag="IXF98_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1555746.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="IXF98_001425"
     CDS             8546..9535
                     /locus_tag="IXF98_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1555747.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="IXF98_001426"
     CDS             9649..10644
                     /locus_tag="IXF98_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1555748.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="IXF98_001427"
     CDS             10648..11790
                     /locus_tag="IXF98_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1555749.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIIRVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="IXF98_001428"
     CDS             11787..13214
                     /locus_tag="IXF98_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1555750.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="IXF98_001429"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="IXF98_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="IXF98_001430"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="IXF98_001430"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ1555751.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="IXF98_001431"
     CDS             15286..16677
                     /locus_tag="IXF98_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1555752.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="IXF98_001432"
     CDS             16810..17757
                     /locus_tag="IXF98_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1555753.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17797..18957)
                     /locus_tag="IXF98_001433"
     CDS             complement(17797..18957)
                     /locus_tag="IXF98_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1555754.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19122..20297
                     /gene="mltG"
                     /locus_tag="IXF98_001434"
     CDS             19122..20297
                     /gene="mltG"
                     /locus_tag="IXF98_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1555755.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20433..20912
                     /gene="greA"
                     /locus_tag="IXF98_001435"
     CDS             20433..20912
                     /gene="greA"
                     /locus_tag="IXF98_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1555756.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21080..21811
                     /locus_tag="IXF98_001436"
     CDS             21080..21811
                     /locus_tag="IXF98_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1555757.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21808..22875
                     /locus_tag="IXF98_001437"
     CDS             21808..22875
                     /locus_tag="IXF98_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1555758.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22882..23514
                     /locus_tag="IXF98_001438"
     CDS             22882..23514
                     /locus_tag="IXF98_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555759.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23654..24319
                     /locus_tag="IXF98_001439"
     CDS             23654..24319
                     /locus_tag="IXF98_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1555760.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24347..24640
                     /locus_tag="IXF98_001440"
     CDS             24347..24640
                     /locus_tag="IXF98_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1555761.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8419 a         3662 c         4781 g         7803 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagagta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaaaaaccag catcctgctg gtttttttgt ctcgacttag ctttttaaat taacaccttc
    17821 catcagttcg taaatctgat ttcttaaatc agtggccggc aatttgatgt ttttatcaga
    17881 ttggatatta ctttccatga cgattgcatc ttttgtgtct tttttcatcg ccgctactgc
    17941 atggaatcga ttgatatttc cctgagcaag gatataatca tttccactgt aaacaccgcc
    18001 gctgtaagga tgtttatcac cattattttg ccacaatgag aaaagtagtt cttttggtat
    18061 aatatcgcca gacagaacac tggtaaagaa tttgttcaag tcataaacag acatacttat
    18121 atttcctgtg cctaattcat ttcggatgtc tgtttcaggc tcgaccaatg cttttgaata
    18181 atcgtcagtt gcagacattt ggtaagatac agcatgctga ggggaatttt ttacattttc
    18241 ataaaaatct gtatgttcta gatggagtgg ccgaatgatt tcttttttta aaagagtttc
    18301 ataaggctga ttcattagtt tgatcaatac ccctgtaagc aatgtataat ttaatggttc
    18361 ataatgatac gtatcataac cagtaaattt caaatgatga agggcaaact gaatgatctc
    18421 actatctgac ataggttttt ttggagaagc ggttcgatga agaccagagc gcatgtaaag
    18481 catatttttg attgagattt gctgacttcc aggaatctga ggataaaatt cagatagctt
    18541 tgtgtcaaaa gttatcttgc cagattgaat ttgttttagg ataagtaaag cagtcatcgc
    18601 tttttggatc gaaccgattt gatagtaaag agaaggagaa ttcgattctt ttatgcgtag
    18661 atttgcgtac ccatatcctt tttctaatat cactcgatta tttttgcgta cataaatcga
    18721 accgatataa tctgattttt gcagtaattg atcaattttg ctggcaagca tctgctcatt
    18781 agatgttaaa ttggaagcaa gttgtgcggg ttccgtttta taaatttgtg gttgaggttg
    18841 ttttgttaat tgccatcctg taatgatgcc tgttgaaaag aagctgacag ccagcaacaa
    18901 aactgcgaga aaagaaagag agatctgctg ttttttttga tgttttcctt ttgccatatt
    18961 aaatcctttc tatccatttt taactatttt tcattgtaat cctcaaaaag agcatttgcc
    19021 acttacaatt ttgacataat atttggtaaa ataattcacg gcagtagaaa tttcatgtat
    19081 aataaacaga gttacgaaga tgatagtaag gaggagttgt tttgtcagga aacaaacaac
    19141 acccaagcaa agacaaaaaa gaagccagta aggcagctac agcagatata atcgacaagc
    19201 ggagaaatct gcgtaaaaaa gaagataaga tcgtgaggaa gatcatttta gtgatcgctc
    19261 ttacattatt gattattggc ggatttctcg ggtttaccgt atatcgttat gtagatagcg
    19321 ggttgaagcc tttagataaa tcggacgacc aattagtcca agtggaaatc ccaaacggct
    19381 cttctaataa gcagattggc gaaattttgg agaaagacaa tattattaaa agtgggattg
    19441 ttttcaatta ttatacaaaa tttaaaaact taactggctt tcaagctggt tattatcagt
    19501 tagcgcctaa tatgactctt gatgagattg gcaagcagtt gcaagaaggc ggcacatctg
    19561 agccaacaaa agttgcagat gggaaaattg ccattccaga aggctacgac atcgatcaga
    19621 ttgctgagcg ggtagcgaag gtaacaggta aagacaagaa agaattttta gatttagtaa
    19681 atgatgagac tttctttaac gagttgtatc aaaaattccc agaattgttg gaaagtgcct
    19741 ctaaagctga gaacacaaaa taccgtttgg aaggctactt attcccagct acttatgatt
    19801 actacacgag tacttcgctt aaggatcttg tgattgagat ggtgaacaag acaaatacgg
    19861 tcatgcaaaa ctattattca gctatcaaac agaaaaattt gactgtacaa gaagttttaa
    19921 ctttggcttc tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aatattgcac
    19981 aagtcttctt taaccgaatc aaggcaaata tgccacttca gtcagatatc tctgttttat
    20041 atgcattagg agaacataaa gaattggtga cgtatgaaga tacagctgta gattcaccat
    20101 acaatttgta taccaatacc ggctatgggc ctggtccttt tgacaatcca agcgaaaagg
    20161 caatcaaggc agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga
    20221 gcactggaaa tgtttatttc gctaaaacgt atgaagaaca tatggaacta gtgcaaaaat
    20281 atgtaaataa ttcttagaaa aacaggggct agtcattgat catttttaga aaaacattta
    20341 cattttaaga aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg
    20401 cggtatttta tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc
    20461 tagaaggtaa agaaaaatta gaaaaagaat tagaagaact aaaaacagtt aaacgcggag
    20521 aaatcattga acgaatcaaa atcgctcgcg gtttcggaga tctttccgaa aactccgaat
    20581 acgaatcagc taaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata
    20641 tgatccgttt tgcacaaatc atcgataatg acggcgtaga tgcagatgag gtatcgatcg
    20701 gtaaaacagt tacgttcatc gagttgcctg atggagatga agaagagtac acgattgtcg
    20761 gaagtgctga agcagacccg ttttctggaa agatttccaa tgattctcct atcgcgcaag
    20821 cattgattgg caaacgtaaa aatgatgaag taaccattgc tacaccaggt ggcgatatgt
    20881 tagttaaaat cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc
    20941 ttactgatga cgcactctag atcttttctc tttgagtatt gcaggtttca ctgaacgatc
    21001 ttacatctaa agtcctatag ccaacagtca aaaaaatagg tatgatgaaa tgagaatgtg
    21061 tcaggaggga gaaaaagcga tgaatagttc ttggcgtttt tttgtggtag tcgaagcact
    21121 gctgctgtta tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtaat
    21181 attcggtatt ttcaatatat atctagctat gcgcaaatat cctcgtacta aatttcaaaa
    21241 ttttcaattg atcttaggga gcttggtcat tttttttagc ttagtcaaca gtcctgcttt
    21301 atggatgatg gttgtattgg ccgttttatt tatcgggctg aaaggtgtag agatttcagg
    21361 aatagatttg acaaaaaatg ctttttggcg aaaaaaacag atcatgatgg tccaaacaga
    21421 gcaattaaaa acacataata atgagcgtaa aagacagcaa ttatttggta accaacgtat
    21481 tggaaacgac gtctatgaat gggatgatat caacattgcc atcatttctg gtgatactat
    21541 catcgatttg ggtaatacac ttttgccaaa agatgataat attgtgattg taagaaaagg
    21601 gataggcaga acacggatct tagtacccct gggagtggct attcgtttag agcatgcgac
    21661 attagtagga aatgtcttgt ttgaagaaga gcagttttcc ttaaaaaacg aacagatcaa
    21721 aatctgcagc agtgattatg atgaaaatcc tcgcagattg aagatcatta ccaatacctt
    21781 ggttggagat gtcgaggtga tacgaatatg atgggaagaa tatccaaagc aatgctagct
    21841 gtttattcgg ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat
    21901 gccagtaacc aaagtcattg gtggggagaa ttgctgcgtg cgcgtttatt atacgttccg
    21961 cttatcttcc atttgatggc aatttcatta ggtgtaggat tgatcgtatt tctattgtta
    22021 tcacttattc aaaaggcaaa atacgggaaa atcgaagaaa agcttcgtgc actttcttct
    22081 ggcaattatg aatccaaact tttgcatcag ccgatcccaa gcgcgtcgga tgatctgtac
    22141 atcaaagata ttgatatgga aattgctaaa ataaaagaaa aaatgatcga agtatccagt
    22201 gaattacaga ttatgactag ccgtcctcaa tatgtagatg gacaaaccaa agaagagatt
    22261 ttagaattag aaaggcatcg attagctcgt gagcttcatg attcagtttc acagcaatta
    22321 tttgcagcga tgatgatgat gtcggcattg acggagcaag cagaaaaaag tgagacacca
    22381 gaaatgttcc gtaagcagtt gaaaatggtg gcagagatca tcaacgcttc ccagtctgag
    22441 atgcgtgcgc tgcttcttca tctgcgtcca gtcaatttag aagaaaaaag tttaaaacaa
    22501 ggcattgagc agttattgaa ggaattgcag aataaaatcc agatttcgct aaaatgggat
    22561 gtagaagatg taaaattaac tagttccatt gaggatcact tgttccgaat cgttcaagaa
    22621 ttgttatcaa acacattaag acatgctaag gctaatgaat tagaagtcta tttgcataaa
    22681 atagataaca atcttttatt acgtatcatt gacgacggga caggatttaa tatgaatgaa
    22741 accaaaacgg gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga
    22801 acagtaaaaa tcattagttt taaagggcaa ggtacgagcg tggagatcaa ggtccctttg
    22861 atgaaggagg catagataaa gatgataaaa gttttattag tagatgacca tgaaatggta
    22921 cgcttaggcg tctcttctta tttatctatc caagaagaca ttgaagtgat tggagaagct
    22981 gaaaatggac gtcaaggcta tgaaaaggcg atggcacttc gaccagatgt cattttaatg
    23041 gatctagtca tggaagaaat ggatggcatc gaatcaacaa aagcaatctt aaaagattgt
    23101 ccaaaagcca aaatcattat cgtcacaagt tttattgatg atgaaaaagt ttatcctgca
    23161 attgaagcag gagcagcagg atatttgttg aagacgtcta ccgcacatga aatagctgat
    23221 gctataaggg ctacgcaacg cggcgagcga gtattggaac ctgaagtgac aacaaaaatg
    23281 atggaaaaaa tgagtcggcg aaatgatcca gtattgcatg aagaattaac gaatcgagaa
    23341 aatgaaattt tgatgctgat ttctgaaggg aaaagtaatc aggaaatcgc ggatgaattg
    23401 tttatcacac tgaaaacagt taaaacgcat gtgtcgaata tattagctaa gttagaggta
    23461 gaagaccgaa ctcaagcagc tatctatgcg tttaaacatg gtttggtgaa ataaaaacga
    23521 tgataaagag gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca
    23581 ataatgatgc ttgcttttgt ttttggaaag gctttgttat actgagaaaa gaagatacta
    23641 aaggaggaaa atggtgaaac aaaattttgc aattgtaggc cttggacgat ttggcggaag
    23701 catctgccgt acactgatcg aagctggaca agaagtactg gctattgaca gtaatgaaga
    23761 tcgagtcaat gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga
    23821 gatgacactt cgttcattag gtataagaaa tttcgatcat gtaattgtcg ctattggaga
    23881 agatatccaa gccagtatct tagttactct gatggtaaag gaaatgggcg taccgaatat
    23941 tttagcaaaa gcccaaaatg agtaccatgc ccgtgtacta gagaagatcg gagcagatcg
    24001 tgtcgtacac cctgagcgag acatggggat acggattgca cataacttag tatcaaagaa
    24061 tattctagac tacttagagc tgtcagataa atattcttta gcagaaatcc gagtctccaa
    24121 tcctaaattt ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac
    24181 agtagtcgcc atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata
    24241 tgttcgcgaa aatgataatc tactggtgat tggagagaca ggcgatgtgg atattctaga
    24301 cgataagatg aatcaataga aagaaaagag aaagtaggtt gttgggatgg aattaacggt
    24361 aacgccaaaa gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat
    24421 acgttttttt ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat
    24481 gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa atggatggga cacttttttt
    24541 tatcgaggaa gcagatgaat ggttctttaa aggatatgat ttacttgttg attatgatcc
    24601 caacctggag gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa
    24661 gtttt
//
LOCUS       DACETN010000040        23953 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DACETN010000040 DACETN010000000
VERSION     DACETN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.041"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(290..595)
                     /locus_tag="IXF98_001441"
     CDS             complement(290..595)
                     /locus_tag="IXF98_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1555762.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(620..1051)
                     /gene="ruvX"
                     /locus_tag="IXF98_001442"
     CDS             complement(620..1051)
                     /gene="ruvX"
                     /locus_tag="IXF98_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1555763.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(1048..1314)
                     /locus_tag="IXF98_001443"
     CDS             complement(1048..1314)
                     /locus_tag="IXF98_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1555764.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1569..2294)
                     /locus_tag="IXF98_001444"
     CDS             complement(1569..2294)
                     /locus_tag="IXF98_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1555765.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2655..2725)
                     /locus_tag="IXF98_001445"
     tRNA            complement(2655..2725)
                     /locus_tag="IXF98_001445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2691..2693),aa:Gly,seq:ccc)
     gene            2842..3594
                     /locus_tag="IXF98_001446"
     CDS             2842..3594
                     /locus_tag="IXF98_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1555766.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3610..4089
                     /locus_tag="IXF98_001447"
     CDS             3610..4089
                     /locus_tag="IXF98_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1555767.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4340..5920)
                     /locus_tag="IXF98_001448"
     CDS             complement(4340..5920)
                     /locus_tag="IXF98_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1555768.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5913..6656)
                     /locus_tag="IXF98_001449"
     CDS             complement(5913..6656)
                     /locus_tag="IXF98_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555769.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6832..7608)
                     /locus_tag="IXF98_001450"
     CDS             complement(6832..7608)
                     /locus_tag="IXF98_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555770.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7856..8692)
                     /locus_tag="IXF98_001451"
     CDS             complement(7856..8692)
                     /locus_tag="IXF98_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1555771.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8703..9632)
                     /locus_tag="IXF98_001452"
     CDS             complement(8703..9632)
                     /locus_tag="IXF98_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1555772.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9824..10177
                     /locus_tag="IXF98_001453"
     CDS             9824..10177
                     /locus_tag="IXF98_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1555773.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10251..10907)
                     /locus_tag="IXF98_001454"
     CDS             complement(10251..10907)
                     /locus_tag="IXF98_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1555774.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11133..13067
                     /locus_tag="IXF98_001455"
     CDS             11133..13067
                     /locus_tag="IXF98_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1555775.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13153..14376)
                     /locus_tag="IXF98_001456"
     CDS             complement(13153..14376)
                     /locus_tag="IXF98_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1555776.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14370..16268)
                     /locus_tag="IXF98_001457"
     CDS             complement(14370..16268)
                     /locus_tag="IXF98_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1555777.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16262..17380)
                     /locus_tag="IXF98_001458"
     CDS             complement(16262..17380)
                     /locus_tag="IXF98_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1555778.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17463..18296)
                     /locus_tag="IXF98_001459"
     CDS             complement(17463..18296)
                     /locus_tag="IXF98_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555779.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18289..19206)
                     /locus_tag="IXF98_001460"
     CDS             complement(18289..19206)
                     /locus_tag="IXF98_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555780.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19433..20557)
                     /locus_tag="IXF98_001461"
     CDS             complement(19433..20557)
                     /locus_tag="IXF98_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1555781.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20740..21648
                     /locus_tag="IXF98_001462"
     CDS             20740..21648
                     /locus_tag="IXF98_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1555782.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21789..23402
                     /locus_tag="IXF98_001463"
     CDS             21789..23402
                     /locus_tag="IXF98_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1555783.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7461 a         4672 c         4015 g         7805 t
ORIGIN      
        1 ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt
       61 gtgaccgagg agttggcagg agaacaccgt ttaaggaaga gactgtgaag tcaacgtttt
      121 gacctgatta ttagagaaac aaatggcaaa atagttcttc atatttcgcc gtttttcgag
      181 cgaatgccga ggggcagctg attgaacatc gtttatttgg aaaaagggat tgtgacaaga
      241 cgtttgtcac aatccctttt tttatatctt attaattcat tcttttgttc tattcttctt
      301 cttccgccat gaatgtattg aatacttctt caatcatgtc ccattctgcg tctgtttcga
      361 tttgttctaa ctcgccttct gttccatctt cgttttcgat gtatgcataa gcttgtaatt
      421 ctacatcttc atcttctgaa acaccagcag gatataaaag aacatagtta cgtccaaatt
      481 cttcttgtcc gtcaactgtt agtaggattt catataatgt ttcatttcct tggtcatcca
      541 ccaatgtgat gtgttcgtgt ccttcatggt cgtgggaatg gttatgttct gtcataagat
      601 tacctctttt cttttattat taaaataatt gcttacctga gccgtctaaa taattttgta
      661 agatcatcac agctgctaat ttatcgatga ctttttttcg tttggctctt gaagtatttg
      721 cctgctctac tagcatacgt tctgcctgaa cagtggttaa tcgttcatct tgataatcaa
      781 ctggcaagga gaatagttct ttgatctttt ctccgtatga catggaagct tctgcccttg
      841 gtccgatgga attgttcata tttttaggta atccaactac aaaccggtct acttcatatt
      901 gtgctactaa ttccttcaac cgatcaaagc caaactcttc ttctgcttca ttgatccgaa
      961 tgatctctac tccttgagct gtccagccaa aggggtcact gatagcaaca cctactgtct
     1021 tagaaccgac atccaagccc attactctca taaattcact ccatgatttg agaggtagtc
     1081 cttgacaatt acttccatga tctcatcgcg ttcgtgacgg cgaatcaaat ttcgagcatc
     1141 acgataacga ggaatgtagg ccgggtctcc agaaagcaag tagccgacaa tctgattgat
     1201 tggattgtag cctttttctt ccaaagcccg ataaacaatc gctaatgttt cgctcacttc
     1261 ttttttgcga ctatcgtcaa aaccaaaacg tacggtttca tctgtaaaac ccatgattct
     1321 acacctcttt cccgcaaggt aattccagct taccttctac aaccttattt tactcgaaga
     1381 aagggaaaac tacaaacaaa tattccaaac ttccgaataa attaagaaac actatttgta
     1441 tgtttttttc cttttttctg tagctttatc aaaagtactc ttttcataaa gtatacgcta
     1501 tatttctttg aatgtatgtt tttacacaat tttttcataa tattcataaa aaattcgaat
     1561 agctgctact aattttttgt ttttgttgct tgtcctgtct cataattgat cgtgtagcca
     1621 tcttgaacat tgtagataaa gacattgaat tcaatttttt tgtcttcaat gctttctgct
     1681 tccaattgca ctcccctggc taccagttca ttcccctcaa aatgaggagt gacacggtag
     1741 cgtacatgat gatttgtttc tttgatatat gccgcaatat cattttcgtg agcaagcatg
     1801 tatggagcat tcaacgaacg tgtccctgtc atcaagtttt taatgttgtt attttgacca
     1861 gtcagctgga aaccaattaa atggctgcga ttatataacc attgcccgtc acttaatttc
     1921 ttttgtttcc aacctgtcgg gtcaatatac aaaggctctc tcttttcagt agggaacaaa
     1981 tcttttccaa gcatagcatt ggcttgtcct actctgttca atttatcaat atttgaatag
     2041 ctttcccatg ttcctttgga aaggctaaga tcctcttttg taaattccgg ctgattccca
     2101 ttgatttcta tttcttgatg ttttccatca tattctggca catccgctac ttgagaagct
     2161 tgtggtgtgg aatcaaaaat agttgtaatc tgctgaaaga tatctgaatc tttttgtgtg
     2221 gtggaaacac cgaatgcccc tgcaatcaca gcagcgacga tccccacgat cgccttggtt
     2281 gtttttttgc tcatttcttt actcctttac taacagctcc ctaaatctta acatatgttc
     2341 ccctataaat aaatactaga aagaaataga aacagcaaaa aataaaacct gttttaagat
     2401 ggcttaaaaa tacaaaaaag agacggattc ctcgtctcta atcagtcacc actctatctg
     2461 taaggtattt attattattc aaaaattttc ttcatactca agacaataca ctgatttcac
     2521 aaccgcttgg taaacggtgc gcaaaagctt gcatctgcgg taataagtcc atccaagtta
     2581 aaaacataag aagttgtttt tgcttggctg ttcttattac ttgttgcaga gcagcttttt
     2641 cacaacctat tttaagcggg tgacgggaat cgaacccgcg acatcagctt gggaagctgg
     2701 gattttacca ctaaactaca cccgcaatac aaaaacagta tacgcctctt ttattccttc
     2761 ctcaagtttt tttattcttt acgcttctgg tatgttttgt agtaaaataa aactaatcaa
     2821 tcaattattg gagaagatgc tatgacggga aaagaattag atctgttgct ggacacttgt
     2881 ttactcgctg ggaaaatcat gatggaaagc aatgcggaga tgtatcgggt agaagatacg
     2941 atgagtcgaa ttgctttagc ttctggaaat ttccgtttgg tcagctatgt cactcaaacc
     3001 ggtctcttta ttggtcttga tcgtacctcg accatccgaa tggaacaaat taccaaccgt
     3061 tcgattaatt tagaaaaagt agtaaaagta aatgatcttt cacgtaaata tgttgcttca
     3121 gagctaacat tagaagaact ttactgcgaa ctgcaagcaa tcgaccgaga ccgttctttc
     3181 tttcctgtct ggttgcaaat cgttagcgcg gcagtgatca gtggaacaat tatggtgtta
     3241 tttggcggaa agatgaccga tttacctgtg actttagtca ttggaggatt gggatacgca
     3301 gcttatctat atagtttgaa attttttaga atcaaatttt tatcggaatt tttatcttct
     3361 ttattgattg gcatagccgc tatctacagc gtacgttttg gcttaggaag caatcaggat
     3421 ctaatcatta tcggatgtgt gatgccttta gttcctggag ttcaaattac gaacgctatc
     3481 cgtgatttgc tagctggaca ttatgtctca ggagtttctc gcggaacaga agcgatgatg
     3541 actgctacga tgatcggttt tgcgattgct tttattttcc agctttttta ttaggaaagg
     3601 aacacaagta tggggacgat atttattcag ttttctttta gttttttagc gactgcggca
     3661 tttgccgtta ttacaaatgt acctaggcgt tcattgattt gttgtggtct ttctggtgct
     3721 tttggctgga tgatctactg gatctgtgtt caaataggcg gatctgctgc ttttggttca
     3781 ttgcttggtt ctttaggagt agcattcatc agcgatcttt tctctaaacg tttgaaaatg
     3841 cccgtaacga ttttcaatat tcccgggatg gttcctctag tccctggtgg tttagcttat
     3901 caagcggttc gaaatctcgt tactgaatcc tatcgagaag cggctcatta tgcggtccaa
     3961 gcaattatga ttgctggagc aatcgcttta ggtcttgtat tatcagaagt ctttaaccat
     4021 aacatccgta atttcaaagt aaagcgtgac gcaatcatga tgaaacgaaa aaaacggctt
     4081 tttaaataaa cgaagtgtaa caaagaaaag gttgtgaaag agacgttcag ccttgagcga
     4141 tgacaaaata tctcacaaac attgcgacaa gtaaccgcag gagcaataag aagtaattat
     4201 gagtattaaa aaaagttttg taaaaatagc ttctcacatt tttacataaa attgacttaa
     4261 tactgagaaa ctgacttttg aacaccctct atctgtaaaa aaggggctgc gacatgattt
     4321 ttgtcacggc ccctcttctt tattcaaatt ttttccaaaa cgttcgttga taataaacga
     4381 tccatagagc ggaaagtatt agaactaaac cgatcacagg aatattcact aaccaaaatg
     4441 gataaaacaa agccaacatt ccgtataaga cgataagaat aatactgatg atcaatgaac
     4501 ccatcatagt tgcgactaaa cccaaacttc ctgaacctct gttaaataat tggttaatat
     4561 tggtccagtt cagcagcagc aaccgataat ctctagcgaa ataacgtagt gacaataagt
     4621 agcagccgaa taaagaacca aatagcaagc tagggataaa aaacaaaggc agctgaaaag
     4681 acagtccgct taataatgcg atcccaccag ttaaaaaact ctgcaatatc catccgaatc
     4741 tgaacttttc ttttaaatat tggttcatag agattggcaa agaacggata aacagaaaat
     4801 tttcttgatc caaagaaata agattgctga taaaagaagt ctgattaact gttaagaaag
     4861 ccaatgcgat ccctaccagg aaaacaacac cgattaactt attatctaaa tcagttaatt
     4921 ttatttcgcc tcctagggca aaagcaagaa taaaaatgat tggagttaac aaggaagaag
     4981 aaagaacttg cataatcaaa ttaggttctt ttactaattg agcattgtaa ctaaataaaa
     5041 gctgatgcac attttggttc tttttatggg ttctttttat tttgaactca ctgtttgaat
     5101 cagtcagttg ctctgtaagc tttggtacta tcagctgttt tacagcaagg aaaaatccca
     5161 gagccgtgat agctagtatt ccccaaccca ataaaccttg ttgagaaagt ggctgactgg
     5221 cagcatcata gaatggcaat aatatcgaaa tcggatgccg atctgttaac tcattttcaa
     5281 tagtccccgt ttgatgattc atccacatga ttccagcaaa tgcgatcagc atcgagctgc
     5341 caagcaagat actagaaact gtttttctat gttttttaaa cactgctgtt cgagccaaac
     5401 caaaaacaat caaactagcc agacaaaaaa gaataatcaa aaatagagaa aacaaaagga
     5461 ttgagataat gatagtaagc aaaatcccta gatcagctcg ccatccggtt aaaataaaca
     5521 tgactagaag cggaaaaaca aaaggaatga tggtcaaagc aactaccaaa atctttgctg
     5581 aaaaaacctg ttcttgtcca aaaggcagag gcaaaaatac ttgcaggtca tttccttcaa
     5641 aaaagatatt ataaatgact gatatacttt gagttaaacc taacatacca aaaagtgcca
     5701 cgtaataagt aaagaaacct ggcaatttaa caaagttcat agcaaacata gcaaatccgt
     5761 aaatcagtaa aaaaatcagc cctgaaaaaa gattttgaga caaaagatag atagttaatc
     5821 gctttccctt ttttcctttc tctcttgctt tattcgtgag ctgtggatta acatataaaa
     5881 gattaactcg aattaattct aataattgtc gcttatacat acggtccatc tcctgtcgct
     5941 tgccttccag ccattttcaa ataaatcttt tctagcgttt catcaggtga gccattcaat
     6001 aattcactaa ctgccccctg ataaatcaat tcacctttct tcaaaatagc cagtttatcg
     6061 cataactgtt gtgcggtatc caatacatgt gtagaaaaaa tgactgtttt tccttttgcg
     6121 gcatgagcct tcatcatttc tttcaaatca aatgctgctt gaggatcaag tccttgcaaa
     6181 ggttcatcca atacccaaat atctggatct ggcagcaacg tgccgatcaa aatcgttttt
     6241 tgacgcattc catgtgaaaa acttgcgatt gtttcatttt gatgactata catatcaaaa
     6301 agcgctgtca attctgctaa acgcttttct tttttttgtg tgtctaattc ataggcagct
     6361 gaaatcaagt cccaatattc tccagccgtc aattgtaaaa aaatatctgg ggaatcaggc
     6421 acataaccga ttttttgttt gatactcaat cgattttccg tcaatttcat tccatctact
     6481 aaaatcgttc ccgatgatgg ttcaataata ctaactaaac ttttaatcgt agttgatttt
     6541 ccggcaccat tatgtcctaa aaatccaaaa atttcccctt gtcttatcga gagagacaag
     6601 tcttttaatg cttgtttttt cccgtaactt ttacttacat tgatgaattc aatcatttct
     6661 gtactccttt tgtttgttat tactttttat tgtacccttc gttattggtg gagtaaatgt
     6721 caaaaagagt gcataacaaa aaaggataga aacgactagg ctgctacttg ggttgttctc
     6781 ttctacagga aaacactctt ctaacaggta agatatgagt ctttgttttc tttaaaatga
     6841 ttcattttct atcttttctt gcataaaatt ctttagttta tcaatacttc tctttcccat
     6901 ctctagttgt tctccatttg caaatgttac tatttcgttt actctgctaa tagatgtaat
     6961 gttttcagta ttaatgatat aagcttttaa atctttacat aaatactcat attcgagtga
     7021 ttttttatat tttttaattg tgccgtttac gagaatctct tcatctttca atactaggcc
     7081 tattttattt ttataacctg gcatgctcat tagataaagg atttcatcgt aatggatata
     7141 gtgatcgcta ttaccataac ggacagttgt ccaccttttt ttcgtagcat gttgaacagg
     7201 atagttttct ttttctttca ataagtaact ttggatgctt tttttatttt tttcataagt
     7261 gaattctttt atcaaataac cactaggcgt aatctttcga ttaataacag ttattgcttt
     7321 attcggatga gacgttagat aaacaatttt acacttttca ttcttttctc taagcatttc
     7381 gcctagttcg ataccgctat aataggcgtt caaatcgatg tctaggaaaa aaatatctga
     7441 ggaatagatt tcattatttt taatgaattc aacaaattgt ataacgttca atccctgaac
     7501 gatctcaaat ttccaaggac ttaataaaat tctggattct tccaaaattt ctactagcat
     7561 tttttcaaaa accaaactat catccaaaat aaatatacgc gttaccataa aaagtacctc
     7621 cactcgatat tttactatca aaatcatttc atacaacttt tcaacataca tgttaggaat
     7681 aaattgtaat tctcttcaca atcttttcca acacctttga aaccgatatc aaatactttc
     7741 aggatgctgc ttctaaaata aatatcaaac aaatgaatac atcatgcaaa aaaagccaga
     7801 caaccgacgt ccttgcgtgt cagctgtctg agctatgttt atttattttg ttcttttaaa
     7861 aagaacgata cacatactga tttttcggag cagagacttt ctcagcgttt gtaacaacta
     7921 cagatgggat ttcacgcttg aatggttgat aatagtctga ttggatggtt ccttctattt
     7981 tataccattc atcattttta accgttactc cttccggcat atgcgtcaac aaaccaaaga
     8041 ctcctgaatc tgccacacaa tggataatgc cgaatcggaa caagaagata tctgtattgc
     8101 tatcgttcgg tgtttggtaa acaaatcctt catagctgat ttttttgcca atgaattcgc
     8161 tagggtaatt ataaattagc tccataactt ctaaatagtt ttcatctgtg atcttgatag
     8221 tttgcttatc cccatacttt tctttcaact cattcatttg gtctaagtag tctgatttgt
     8281 tgaaataaat actggtatcc ggctttaagt attgtgtctg catctccgga tcccctactg
     8341 attctttact tactgggaaa ttaaatcctt tggcttctac gatcgtagtg tcgagactaa
     8401 ctgttggaaa caacgtcccc acgattactg gcaaagctag cagtacataa gcgattccac
     8461 gttgataagg ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt
     8521 ttccgttttt gacccacaaa tacagctgaa ctaaagccaa aagagcagat aaaatcattg
     8581 aaagaatagc taagtagcga taatggacat tgatatattg atcgagtttg ccagaaactt
     8641 gcaagtacat catcaattcg aaatacccag ataaaatcaa aaaccgcagc atattttccc
     8701 tcctaaataa ataatgtgaa tccaataacg ctaagggcaa tcagtccgat cagcgataaa
     8761 ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat ttttgatatc gaccattggg
     8821 ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa agaagatcct
     8881 ataaacgcat ctgcttctga gcaaagagac atcgtaaagg ccaataacag catgatgata
     8941 atggctaaaa tctttgttga gccgagcgtt agcataattc tagttggtaa ataagtctgc
     9001 atcgcagctg cgattaatcc gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg
     9061 attgaatgtg tcaacacatg cccagtttga tggatcagct gattttttgg ttcattcttc
     9121 tcactttgtt cttcttcatg gacatgatga tggtgatttt ctgctacttt agacgtgatt
     9181 ttttctttca atacagcgtc tttttgaaaa taggcaagcc agacaccaat cacaagggca
     9241 acaaaaaagc tgcctatcac ccgccagctt gccatctgcc aagaattgcc aaatgcgata
     9301 aaagtggaaa aaatcacgat tggattgatg atcggagcag tcagcataaa agcaaacgct
     9361 gtgtgaactg gtacgccttt tttaacaaat tgatgaacga tcggtacgat gccgcattca
     9421 catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat acgattttca
     9481 ggaagccatc tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc tgagataaga
     9541 cagcctaata atacgaaagg taatgcttca ataacgatcg ataagaaaat cgtactcatc
     9601 tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc tcctttttat ttctagttca
     9661 ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag ttgctctgta aatttttaca
     9721 caactcttat tttttaccaa agcacttcaa ttgacttttt ctgttattcc cattaaatta
     9781 aggtctcata catatcactt ctatcataaa agggagaata aaaatgaaac aaaccactaa
     9841 cttgactgaa gttcaagaca tactgcaaca gtcatccgtt gccgtcattt atctttcgat
     9901 gcctaattgc agtgtttgtc acgctgttcg tccacgacta gaggaacttc tcacacatta
     9961 tgatattccg gcacttcatt tggatgcatt tgaaacacca gaagtagcta gtaaatttga
    10021 agtgctgact gcgccagttg tcctgatttt tcatcaagga aaagaagtct ttcggcaagc
    10081 acggttcatc gactttaaaa aaatccgtta tctactagat gaactgacag cagaagatga
    10141 ttccctttct tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc
    10201 gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg ctttttattt ttattctgct
    10261 gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca
    10321 ttcgtcaaat caacattttg gatcaataca tctgacggag cattcaatcg agcagcggta
    10381 aagttcaaga ttcctttgat tcctgcttcc gttaactgat tgactacttc ttgtgctctt
    10441 tcagcgggta tcgttaaaat tgctacttcg atttgttgga tcttgatttg ttctgacata
    10501 tcttccattg gataaacggg gataccgtct acgatgcgcc caacgatttc ctcattcaca
    10561 tcaaaagcac aactgatacg tatgctatta ctttgatgga atttgtattt tagtaaagca
    10621 cttcccaagt tccctacacc gatcagcgca acatttgtca attgatcgtc gtttaatgtc
    10681 tttgcaaaaa agtgcataag atcttctaca tcataaccat acccacgttt tcctaattcg
    10741 ccaaaataag aaaaatctcg acggatcgtt gcactatcta cttgaacggc ttcacttaat
    10801 tcagttgaag acactttttt ctttccagca ttatttaaaa tccttaaata gcgataatac
    10861 aaaggcagtc ttttagctgt cgcctttgga ataatatgat ctttcacgaa atgacctcca
    10921 cgtattgtct aattacatct ttgatttctc catcatagca gtatttatag gaaaaaatca
    10981 attccattgc tcagatattg tgaaatttta ttctttattc atttatttat catttctgca
    11041 aatctcgcag tataatggaa tttatggtag actactaatt gaaaaatagt tttttatttg
    11101 aggagaattc acaaagtaga aaagaggatt ccatgatatt attacaagcc aatcagattg
    11161 ctcgccattt tggtgcagaa gtcttatttg aaaacataca tttagaaatc agtacaggcg
    11221 ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc gactttatta aagatcattg
    11281 ctggaattga agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg actctcggat
    11341 acctcgctca ggacacagga cttgattcca atgaaacagt ttggaacgag atgctgaaag
    11401 cttttgaaca agttagacaa atggaagagc ggatgcgcga gctagagatt ttcattagcg
    11461 aaggtacgcc agacacacct gcttatagtt cttatttaaa agaatacgac cggctgcaac
    11521 acgatttttc agatcgaaac ggctatggtt atgaaaatga aattcgttcc gtattgcatg
    11581 ggtttaaatt cgattcttca ttttatgatc agaaaatcga tactttatca ggcggacagc
    11641 gcacacgttt agctttagct cgcatgcttt tgcaacaccc tgacattttg attctagatg
    11701 aaccaacgaa ccatttagat atcgagacac tttcctggct tgagaattat ctgcaaagct
    11761 atactggtgc attattgatt gtttcccatg accgttattt tttagataaa gtagtcaatg
    11821 aagtctatga attaagcaga agaaaaatgc gccattacaa aggtaactat tcgaaatatc
    11881 tagatttgaa agcagaacag ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag
    11941 aaatccataa attagaagac ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac
    12001 gagcgcaaag ccgcagaaag acattagaaa aaatggaccg gctggatcgt cctcaagggg
    12061 atgaaaaaca agcaaacttt ctattcagta tagaaaagcc ttctggaaat gtagtcttgc
    12121 aaatcgaaga tgccgctatc ggatatgata atgtcacctt atctgaacca atcaatctag
    12181 atatccgtcg tcaagaagcg attgctctag taggtccgaa tggtatcggg aaatcgacgt
    12241 tgctgaaatc aatcattcac cagatccctt ttatccgtgg aaaagaaacg ctagggacaa
    12301 atgtcaccat cggctattac gatcaaggtc aagctgatct gcatacaaac aagacgatcc
    12361 tagcagaatt gtgggatgag catcctacca ctccagaaaa agagatccga agtgctttag
    12421 gtgctttctt attttctggc gaagatgtag aaaaaccaat ccctctgcta agtggcggtg
    12481 aaaaagcacg ggttgcttta gccaagttag cgatgaacaa agaaaatttt ttgattttgg
    12541 atgaaccaac aaaccattta gatattgaca acaaagaagt attagaaaat gcattgatcg
    12601 attatgacgg cacactgctt tttgtttccc atgaccgata tttcatcaat cggatcgcga
    12661 caaaagtcgt cgaactctct gaaaatggca gcaaagtcta tctaggagac tatgattatt
    12721 atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa gatgagccag
    12781 aatctgctcc tgctcccacg aacaaatctg attattacca aagcaaagaa cagcaaaagc
    12841 ttatccgtag ccttcaacga aaaattactc aggttgaaga agaaatggcg cggatcgatg
    12901 aatggatcga tcagttggaa caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa
    12961 aactcaatga gctgaaccaa gatttagaag ctgctagaca agatcaagaa acaaaattga
    13021 cggaatggga agaactaagc ttggaattag aagaatatga aaattaacga aaaaaccaaa
    13081 acagaaacca ttcatgctgt tttggttttt ctttttttat tttatcaaac aaaaattcca
    13141 tatttacctt tttcaccaat aagaagacca attactagtc attctcacta aagcttctaa
    13201 ataatagtaa tcaccccaaa tacaaggtgt atttacccca ttatttttgg gcttattata
    13261 aacagactgt gttaaaaagc catcttcttc ttccgttgta gcatatcttt ctttcaattg
    13321 tcgcataatt ttccaaacag cagactggta taagttcttt tctgttttgt ctaatagacc
    13381 tgattctatt aattctaata atccacaaac catgatcacc atactagatg tatctttttc
    13441 ttctctgcta gaatcatcaa agatcaaatc ccaataacaa atggtatctt ctggcaaccg
    13501 attcataaaa taattggcta atattttgct taaatcaaaa tatttttgtt ctttcaaata
    13561 acgataactt aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc aacaggaatt
    13621 atcactataa ccttgatgcg tacttccata tcttggtttt ccatctttga catccatata
    13681 aaatgtatga taagttgaac catcttctct tactaaataa tctgctgcat tttgaatatg
    13741 agcctgagca gcttttttat atttttgatt tccagtttct tcactcgccc aatacaataa
    13801 tggaagattc atgcaacaat ccatgatcat tctaccttgt tgtgaaggat cactaggatc
    13861 tccccaagct tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc
    13921 atcaattgaa gcttccttca tttttcgatt tcccgttatt ttataatctg ctacggttga
    13981 taaagaatac aaaaaaccaa gatcgtgatg attagtgata atttgacagt caatcctgtg
    14041 gataaaatct atatctgctt tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc
    14101 aaataacaac caattaattc ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa
    14161 tggataacgt ccttttatag gacactcgct tgggaattga tactgactgt tactgatacg
    14221 tttgattaac ctctccgtgt gtctgcgaca acgctcgatc tcattattcc attctttttc
    14281 cattaattct ttataacatt catttatcca tttttctgga tgtattatct cttcaaacat
    14341 cactttatca agcatttccc caccagcctt tagcataatt ttagtctttt tttattgttt
    14401 ttattttgga tagttagccc ggttgtccca tcaaaatcaa ttttgggacg attgttccat
    14461 acttctaaac tataatcccg atcttgatga gcaaccactc ctgttccgag ccaatgggtt
    14521 ccttttccaa atgtaccctc taatccagga ataaaagtgt tttcccatgt catcaagttt
    14581 gtattgggca aacccataat agctgtggat tttcgtttaa tagtatcgtc acttaaacag
    14641 ataatacctg aaaagccagc ttcactcacc ctatagtttt ccttgtcttc ttctatgatt
    14701 attccttctt tctcaggaga acattcaata gcaaaccctg tctcatatag aacatattct
    14761 gtatctaatt ctactttgtg tacgcgaatc tgccagcctt caaaaggaat aagccaagtt
    14821 ctgatttttg cctgtaaagt aggttcccat gtagaggcaa gtacctcttt cttacataca
    14881 atgtctcgta cattatttcg aacgatgaac tgtccatagg ttttgatgct ctcattctta
    14941 aaactggtcg tgatatatgt gtccgctcgc tgaatcccta gcattgaatc cattgccatt
    15001 tctgctttca attgattcgc tcgcggtatc gaaaaaccaa attgactaga ataagcaaat
    15061 tttaaatatt tttcattggt caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa
    15121 aaaaatgtat gtccattatc atgttgtatg agcaaattgg cttctgaaag aagtttagtt
    15181 ccttcacgaa taggcatagg catttcttta gccgtccaat actcatggtc ttcaggtagc
    15241 gccaacaata aaaaaatctt attcagccat aatggagata acgtactatt atagggttca
    15301 gtcaatgtca attgagggta ggtataacca atattcagga tacctttttc atcaaatatt
    15361 tctttttgca tccaccatct tatattccga ttgactaacc ccttgatttc acctaattta
    15421 aaaggcaaaa gatcggccca caacatagct gaccaaaagc ttgaaactgc aaaacgataa
    15481 atcatgcttc gaccaaaagg cacatttgca ccatcttccg caaaaaaatg aatatagtct
    15541 ctagaaaaat caatagctcg gttcttatat atctggctaa tgggcttttc tggttctagt
    15601 acgctataaa tcaaaccata ataatgaaat gcaaaaggat tataataatc ttggcgacct
    15661 cttgaactgt ctcgatacca gccattttga agataacaat tttctatatt atcaaatgct
    15721 tctttcagtg ctgattcttt aactggataa ccaaattttt ggataattgt tccgacaatt
    15781 atcttaaaaa attgccaatt accatctgca cattcatatt caaaaatttg cgagaaccaa
    15841 tgaataatct ggcttttttc tcctgtatct aattttttcc ataataattg ttcatgatga
    15901 tataacgcta ataacattgc gggcatttct accatacgct ggtctctttc atgtatttct
    15961 ccccaataat cgggatggga aggatctgtt ccagctttta ttcctgatag aattaatttc
    16021 aaccatcgtt catctaattg gtcaattgcg ggccccgctc cccataaaac ccgagagaat
    16081 ccttccattc ctgcaatttt atttccatat cctacccctt gatgtatata atcaatttgt
    16141 gcgccacttg ctcgataaaa cggctggagt ggttgattta tctttttaaa taagtcaaag
    16201 taatcctgtc tggtccttaa tagtggtact ggatgtttca ttgtattttc tcctcttttt
    16261 actaccaaat agctaattcc gactgttcta atcgaagtaa gccttcaata aaaaaataat
    16321 cagaatagat cagtggcaca ttataagtca gttctctatg gtatgcttct gctccgcctc
    16381 caatgatccc atcttcatcc atattccaat ttgcatagtg cttttctgtc ttttggagta
    16441 tattgatagc agcttcatga tacatttttg atcgtacaga ggaaacatat cctgctaatt
    16501 ccagaagacc gcatgcagcg catactccag cactggtatc aatattcgaa tcttttacag
    16561 caaaatcaat gagaggaata taaccagttt tttcgatttt ctgaataaaa tttactgcta
    16621 tcttttctgc tatagctaga tagtacggat tttttgtgta acgataactt aacgcgaatc
    16681 catataaagc ccatgcattt cctctgctcc aacaagaatt ctcattgtat ccttgcccgc
    16741 caagtaaccg aatatatttc cctgattctg catcaaattc tgcgatatgc ccggttgatc
    16801 catctttcct cactagatac ttagctgttg tatctgcatg ctgccgagca attttagaga
    16861 acctcggatc accagtttcc ctcgaagccc aatacagtaa tggtaaattc atcatactat
    16921 caataatcat ccaaccaggt ttatggtcat tccatgctac taaatagttc cctgaaatat
    16981 tatagcgtcc agcaagtacc gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt
    17041 gagactgttg ataatgtgca atagccgatg gtaagaataa aaaacctaca tcatgatgaa
    17101 cgtctgaaaa atggtacagg gcagattcta ataattgttc ttgttgtatt gctgcctctt
    17161 tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa aataccactc caaaaaccat
    17221 tggtccacca gtcgattcct ctgtccttta ttacgtctgt atattttccg ttgtaagaaa
    17281 tatatggaat tttattcccc attctgtcta attccgcatc tatcttttca gaagctacat
    17341 tcaaaatttt tcttgaccat tccttcgtct gttcattcat ggatatcccg ccattctttt
    17401 aatttttatg aatgtggagt tgctcctaga acgaactaga tggttaacat tcgttatttt
    17461 ttttacttat ttgcagctcc aatgtcattc tccttaaatt tagactgcgc ccacacataa
    17521 atcaagacca ttgggatcac tgaagttgct aaaattagca taattgcgcc ataattaatt
    17581 ggctcacctg tactagaaac cgtcatattt ttgataatta agggaaccgt atatttatct
    17641 ttatctgaaa gcatgactaa tggaaataga tattgattcc aacttcccat aaagataagt
    17701 aaaccactag taataatcgt aggtgataaa atcggtacag cgattttcgt aaaaatcacc
    17761 cactcattgg caccatcgat tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta
    17821 tattgtcgca tcataaacac tgaagttgta ctggcaatcg aagggatgat cacacctgca
    17881 taagtattcg ataatcctaa acgactcata atgtaaaata aaggaatcaa tgtggtaaaa
    17941 aaaggcacca tcctagatag cataactgta ttgaacaaca gtttattccc cataaaacga
    18001 tactttgcta atgcataacc cgccattgta gaactggctg tagctaaaac tgtactgatt
    18061 acagcaataa caatcgaatt ccaaataatc aagggataat tatatctcgc catgatggct
    18121 tgccagtttt ttaatagcat atccccaaac ttgaaattga ttccgccact taaaatttct
    18181 tgattacttt gcgttgaagc tacaatcata tataaatatg gcatcaaaaa tattaaggca
    18241 ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta tcttttgttt agtcattttt
    18301 atcacctccg aatttaaatt gaatagccga taatataaca gatacaaaca ccatcgtcca
    18361 ggcaatagca gaggcttttc caaaatcaac catttcaaaa cttgttttat ataaattgac
    18421 tcctacgacc aaagctgaat tttctggtcc attggaagtc ccaaagaata tatttgggat
    18481 attgaaatta cccaagccat tgatagtggc taaaactgtg gccattaaaa tgattggctt
    18541 tagcatgggg atcgttaata aaaagaattg ccgaaatttc gatgctccat ctatttcagc
    18601 actttcgtat atctctttag aaatcccttg aagaccagct aaaaataaaa tagtgttata
    18661 tcctacacta agccaaatca tcgtgatgat cattgatatt cgtatccaaa aaggatttgt
    18721 taaccactgt acaccgttaa ttcctagcgc attcaaaaat ccattgaata acccagtcgg
    18781 attgaaaaaa gtcatgaata cggaagctac agccaccgtt gatgtaatgg ttggaatata
    18841 atagacagtt ctaaaaaatc cttttccttt taactgtgct gaattcaata aaacagcaaa
    18901 gtaaatagct aatagcatcg ttattggaat tgatcctgcc atgataatca aaacattcgt
    18961 gtaagattta ataaacaatg gatctcggaa gatagctaga taattgtcaa atccaacaaa
    19021 ttcaaatgag tagccaattt tcataaaact aaaagcaaac gagataatga tgggaatcaa
    19081 aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat ccataagcac tttttttatg
    19141 agacgtatct gttgcttttt tcttgctgtt ctttattgtc ttgcttggta acgaagattg
    19201 catcatcgtt aagtcctcct aatatttttt aatcaccagt taattttacg ccgtacttat
    19261 gagcaaaatt ttctgcttct tttagatatg aattttcaaa atcacctgtt tgccaatagt
    19321 tatatgcagc ggtagtcaaa taagacgtag catctgaata agtggctggt aaaacagtat
    19381 ttgatgtatt ttcattgatt ttattcaata gttcatagta tttttgtcct ccttagtagt
    19441 caaacttttt ctcagctgct tctgtttgat aggccaatga gttagatgta gccacgccca
    19501 gctccagtgc gttggctaag cattcttcat ctgtcagcat aaatgtaaga aattgttgtg
    19561 ccacttgtgg atttttggca tctttaggga cataaaaact tgatcctcct gatactgcac
    19621 cttttcctgg attttctttt gaaaaaggaa tcagtggagc catgcgccat ttgtcagatg
    19681 catcaggaaa attcaaagac atattcgtag cataccagcc gccagcaaca aacattgcag
    19741 attcttgaaa tgctttctca gcatcttgtg caccgtagaa atgtacaata tctgagtcga
    19801 tcatttcttt gattaacttt gcagcatgtt tcgcttcttt agtattcaga cggatatttc
    19861 cttcttcatc aagcaaagat tgattctgct gggctagcat gttgataaac aattcttggt
    19921 ccccagattc tggcaaggca attccaaaac taccagtttt ttcttttaat gcttttgcca
    19981 cctggatata ttcttcccaa gaactgattt cttcaaaatc tacacctatt tcagcaaatt
    20041 tttttggttg ataataacaa agtgctgggg taaaatcact agcccaagga ataatcgttt
    20101 cattactcgt ttgttgacgc aaaatattca atttttgatc ataaaactga tcgccatatt
    20161 tgtcataaaa tccatattca tcagcactat aaaaagcatc ttgaaatttt tccagcacac
    20221 ctgctgctga agtgtctgcc atggagataa tatccggcat atcttcgtgt gttaccaatt
    20281 ttggggtgat catttgtact gttgctgttc ccgaagctac attcacaatt tttaagtcaa
    20341 tatttgctcc agttttgcgg ttatagattt ttgcagcatt ttcaacatag tttgtcagtt
    20401 tgccgtttgc gacatatact tctaatgtag tggctttttt tatcattgga tctgcgatgt
    20461 ttgctgttac ttcttcgtta gtttttccac atgcacttaa gctgaataat agggtaatgt
    20521 ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc tatcccttct ttcttttcaa
    20581 gttcttatat aaaacgcttt cacttatgta tattaacgga attagtaaca caaatattta
    20641 tataaaaaaa tattttactt gtcgttttgg cttttcattt ttgttataat agtcattaat
    20701 tataaataga aagcttttta tataaaaaga gaaaggaaag tggttctttt gttaacaaac
    20761 aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt ccatagatac ccatcttcat
    20821 ttatctgctg ctccctattt tcgactacta ataaatgaaa gcgctaattt atatgctgaa
    20881 tttgcacata aatctataga actagataat ccttcccttt tattcattga taaacagcaa
    20941 gaagtccaaa tcttttctac tcaaaaggaa acacaaactt ttatcattat ccagttttat
    21001 tttgatgagc aattattagc acatattacg ggacaatctt tttctataga tcaagaaata
    21061 acatctatca ttacttttct gaaaaatgaa cagttcaaaa ttgattatct aaaacatagt
    21121 aagccagcta tttcctatga tcaggaaatc aattcttttt tcatactatg ccaacagatg
    21181 agcattcatt tgctttcctt attagttact tattgtctgc aaaaaattca attgtcttcc
    21241 ttaccaaggc attatcaaca ggaacttgct ttattgacaa taccttcaaa tgagaaagtc
    21301 attcctaaaa aaaagcatat tctttattca gatgaattag ttgatccaat catacgatat
    21361 ttagaagaac atctttcaga aaattttttg cttgaacaat tagcccaaat tttttttatc
    21421 agtccagcca gcttaaaaaa acattttaga aaagtgaatg gaaaaagtgt tattagctat
    21481 tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc tagaaaatga attaacctat
    21541 acagaaattg cagatatatt aggatttcat tcgatccatc atttttcttc agcttttaaa
    21601 aaatatacag ggttatcccc ttcaaagtat tatctatcct taaaatagaa aaaaattttt
    21661 aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa acaataattt
    21721 tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt agaaaaggag
    21781 atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca tttgccactc
    21841 gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga gaaaatattt
    21901 cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac cgtgactcgc
    21961 tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc tttactaaac
    22021 acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac ctatccacaa
    22081 atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct agaccagatt
    22141 atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt agtgtgacgg
    22201 atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc gtcattcttg
    22261 gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc ttagacgtga
    22321 ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt gctaatttag
    22381 tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga gttactgaga
    22441 tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct gctgatatgc
    22501 taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc ggcatacaat
    22561 tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg ccggacatct
    22621 atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg atccaaagta
    22681 tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta aatggtacgc
    22741 ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat catacagcaa
    22801 gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat tataaaactg
    22861 tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga ttaaatctaa
    22921 agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt ggagaaaaag
    22981 gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg acagtctttg
    23041 atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt gacccgttga
    23101 atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg atcaaagcaa
    23161 gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt gaacctaata
    23221 aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag ttacgagatc
    23281 gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga ggattgaata
    23341 cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg attgaagaat
    23401 aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg aggagtaaaa
    23461 atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat aaatgtgaag
    23521 aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt taaacattgc
    23581 gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac tctgagacaa
    23641 atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag gcatcatatt
    23701 aatctatcag cactagtacc taaactagca tccctttcga atattttttc ttatataata
    23761 aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt gtgatactag
    23821 gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca tcctcgttgt
    23881 cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa tttttttgtt
    23941 cctatttatt taa
//
LOCUS       DACETN010000041        23802 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DACETN010000041 DACETN010000000
VERSION     DACETN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.042"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            161..598
                     /locus_tag="IXF98_001464"
     CDS             161..598
                     /locus_tag="IXF98_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555784.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            693..1577
                     /locus_tag="IXF98_001465"
     CDS             693..1577
                     /locus_tag="IXF98_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1555785.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1801..4968
                     /locus_tag="IXF98_001466"
     CDS             1801..4968
                     /locus_tag="IXF98_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555786.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4988..5533
                     /locus_tag="IXF98_001467"
     CDS             4988..5533
                     /locus_tag="IXF98_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1555787.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5561..6016
                     /locus_tag="IXF98_001468"
     CDS             5561..6016
                     /locus_tag="IXF98_001468"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1555788.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6013..6771
                     /locus_tag="IXF98_001469"
     CDS             6013..6771
                     /locus_tag="IXF98_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1555789.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6857..7597)
                     /locus_tag="IXF98_001470"
     CDS             complement(6857..7597)
                     /locus_tag="IXF98_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555790.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7658..8911
                     /locus_tag="IXF98_001471"
     CDS             7658..8911
                     /locus_tag="IXF98_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1555791.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9233..9460
                     /locus_tag="IXF98_001472"
     CDS             9233..9460
                     /locus_tag="IXF98_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1555792.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9583..9909
                     /locus_tag="IXF98_001473"
     CDS             9583..9909
                     /locus_tag="IXF98_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1555793.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9977..10690
                     /locus_tag="IXF98_001474"
     CDS             9977..10690
                     /locus_tag="IXF98_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1555794.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            11000..11851
                     /locus_tag="IXF98_001475"
     CDS             11000..11851
                     /locus_tag="IXF98_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1555795.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11972..13837
                     /locus_tag="IXF98_001476"
     CDS             11972..13837
                     /locus_tag="IXF98_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1555796.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13885..15294
                     /locus_tag="IXF98_001477"
     CDS             13885..15294
                     /locus_tag="IXF98_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1555797.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15402..16103
                     /locus_tag="IXF98_001478"
     CDS             15402..16103
                     /locus_tag="IXF98_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1555798.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16262..16741
                     /locus_tag="IXF98_001479"
                     /pseudo
     CDS             16262..16741
                     /locus_tag="IXF98_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17589..18164
                     /locus_tag="IXF98_001480"
     CDS             17589..18164
                     /locus_tag="IXF98_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1555799.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18164..18604
                     /locus_tag="IXF98_001481"
     CDS             18164..18604
                     /locus_tag="IXF98_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1555800.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18620..19321
                     /locus_tag="IXF98_001482"
     CDS             18620..19321
                     /locus_tag="IXF98_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1555801.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19809..20234)
                     /locus_tag="IXF98_001483"
     CDS             complement(19809..20234)
                     /locus_tag="IXF98_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555802.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            20608..21579
                     /locus_tag="IXF98_001484"
     CDS             20608..21579
                     /locus_tag="IXF98_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1555803.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            21611..23008
                     /locus_tag="IXF98_001485"
     CDS             21611..23008
                     /locus_tag="IXF98_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1555804.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            23167..23775
                     /locus_tag="IXF98_001486"
     CDS             23167..23775
                     /locus_tag="IXF98_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555805.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8042 a         3935 c         5047 g         6778 t
ORIGIN      
        1 tttttattag aactaaatca acagtagttt aattactact tgatttagtt ctttttattt
       61 taaaaatttt ttttacttag aatccagcga ccctagatat ttttctgtat aatcttaaat
      121 aaagaaaaca atatcaaaaa atgcacgtag aagtagataa atggagtggt tttgcaaatt
      181 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
      241 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtgtt
      301 aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg acatattaac
      361 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      421 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      481 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      541 cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca aaaaataaat
      601 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      661 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      721 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      781 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      841 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      901 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      961 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
     1021 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
     1081 actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat gatgtagatg
     1141 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1201 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1261 gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca ttacttccaa
     1321 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1381 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1441 cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1501 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg ccctcacatt
     1561 tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga acaccgcttg
     1621 ttcgggataa gggactgtga caagactttt gtcacagcct tttttttgta caaaaaagaa
     1681 atttacacct aatagaaaaa tttttccata aaaatacata ttttaaatat gaaagtacaa
     1741 ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga
     1801 atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg
     1861 gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa
     1921 agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa
     1981 agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     2041 gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg
     2101 tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact
     2161 aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc
     2221 gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag
     2281 atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg
     2341 acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc
     2401 agatatcatt ttacgggaga aataaaattt accgggacac ttgatttgta tgatgggtcc
     2461 aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg
     2521 ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt
     2581 agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa
     2641 ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc
     2701 catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat
     2761 gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga
     2821 agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc
     2881 caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat
     2941 acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt
     3001 ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg
     3061 cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc
     3121 gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca
     3181 gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac
     3241 agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3301 ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat
     3361 tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa
     3421 acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat
     3481 gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga
     3541 agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag
     3601 atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa
     3661 aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc
     3721 aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3781 atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga
     3841 gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3901 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca
     3961 gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat
     4021 gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa
     4081 gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa
     4141 gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4201 cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca
     4261 ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa
     4321 acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca
     4381 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg
     4441 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     4501 gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc
     4561 tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa
     4621 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg
     4681 aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt
     4741 tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa
     4801 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat
     4861 ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     4921 tactcagcag agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg
     4981 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat
     5041 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     5101 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     5161 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     5221 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     5281 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     5341 aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa
     5401 agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa
     5461 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     5521 aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta
     5581 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     5641 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     5701 cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     5761 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     5821 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     5881 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     5941 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     6001 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     6061 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     6121 tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     6181 ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta
     6241 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     6301 acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag
     6361 aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa
     6421 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     6481 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt
     6541 tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact
     6601 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     6661 gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt
     6721 tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc
     6781 aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg
     6841 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     6901 tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat
     6961 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta
     7021 aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt
     7081 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     7141 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     7201 cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     7261 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     7321 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     7381 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     7441 aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag
     7501 tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt
     7561 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     7621 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     7681 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     7741 agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc
     7801 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     7861 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     7921 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     7981 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     8041 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     8101 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     8161 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     8221 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     8281 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     8341 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     8401 aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     8461 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     8521 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     8581 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     8641 tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc
     8701 gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     8761 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     8821 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     8881 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa
     8941 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     9001 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     9061 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     9121 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     9181 tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac
     9241 ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt
     9301 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     9361 agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga
     9421 cacgctactg catacacatg gtggctacct aaattattga ttttctatga catttatttt
     9481 aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg
     9541 tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa
     9601 gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct
     9661 atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa
     9721 gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat
     9781 tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg
     9841 atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca
     9901 gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag
     9961 tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct
    10021 cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta
    10081 ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa
    10141 aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga
    10201 aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt
    10261 cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta
    10321 cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag
    10381 ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc
    10441 caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa
    10501 agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact
    10561 aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag
    10621 tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa
    10681 aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa
    10741 tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat
    10801 ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat gaaaaggctt
    10861 gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg ttactgtacg
    10921 gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt ttttattttt
    10981 cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg tcgtcatttc
    11041 gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa
    11101 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgttttaa gagattcttt
    11161 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
    11221 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
    11281 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
    11341 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
    11401 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
    11461 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
    11521 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
    11581 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
    11641 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
    11701 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
    11761 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
    11821 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
    11881 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
    11941 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
    12001 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    12061 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    12121 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    12181 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    12241 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    12301 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    12361 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgaag cagatggtgt
    12421 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    12481 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    12541 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    12601 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    12661 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    12721 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    12781 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    12841 aagtttatgg caagtattcg tgatgttcgg catgcatcgg ggattcgtac cgatcatgtt
    12901 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    12961 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    13021 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    13081 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    13141 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    13201 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    13261 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    13321 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    13381 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    13441 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    13501 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    13561 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    13621 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    13681 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    13741 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    13801 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    13861 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    13921 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    13981 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    14041 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    14101 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    14161 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    14221 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    14281 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    14341 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    14401 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    14461 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    14521 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    14581 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    14641 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    14701 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    14761 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    14821 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    14881 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    14941 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    15001 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    15061 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    15121 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    15181 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    15241 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    15301 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    15361 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    15421 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    15481 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    15541 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    15601 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    15661 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    15721 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    15781 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    15841 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    15901 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    15961 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    16021 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    16081 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    16141 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    16201 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    16261 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    16321 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    16381 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    16441 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    16501 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    16561 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    16621 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    16681 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    16741 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    16801 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    16861 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    16921 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    16981 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    17041 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    17101 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    17161 cagctattta tcgttaacaa aaagattttt aggagaagct gatgaacaaa aatgatcaaa
    17221 ttttgatcgt ttagttcgtc ggctttttta tttagagaaa catcatttat cagtgaaaca
    17281 taacacgaga acaaaatgat tttttcgtta ataaaagaaa caagtatttg ttgtttaaaa
    17341 taatacacgt tattggcata taggacgaaa tcccagtatc ttctataagt aagtcatttc
    17401 atatcgatga aaacacgtta tttatttttg ttttatctat aaaatggctg aaaaatgatt
    17461 tttttctaaa tactatatat agtggcgtct atcctatata ccactaaaaa actacaaaat
    17521 ctagtctttt attttaaaaa aacacgtgta gaatgaagat agacttttga gttagggagt
    17581 gtttttttat gaaacttact gttttttgcg gttcccgttt tggtaataaa gaaagttaca
    17641 aatttattgc gcaaacgtta ggaaaataca tggcacaaga agaaatcgaa ctggtttacg
    17701 gtggttcata ctccgggatt atgggagtga ttagtaaaac agtactggaa aataacggaa
    17761 aagtcacagg tatctatccg aatgggcttt ttgaaactga attgcctgga aaagatgtta
    17821 ctacatttat tcctacagaa acgatcgatg aaagaaaggt attattattt gaaaagggag
    17881 atgctgtcct cgttttccct ggaggtctag gtacattgga ggaattctca caacttcttt
    17941 cttggatagc aatcggtctg acgcccgaca agccaatcgg aatattgaat attggcggtt
    18001 attacagcgg cttgcaacaa ttattagaaa cttttgcaaa agaggatttt atggataaaa
    18061 aatggttgga tcaagtaatc ttttccaaca atccattgga actggtcaat gaattacgag
    18121 cagtatctat taaaaatcag ttattattga aggaggcaaa ataatgagtc aaatatattt
    18181 agcaggacca tttttttcag aggagcaaat cgatcgagta agtcgtatcg aaaaagcgtt
    18241 ggaggaaaac aaaacagtca caagtttcta ctctccacgt catcaccaag aaagcaatta
    18301 tgaactattc agcgctggat gggcgcagga agtatacgaa aaagatatgg aagaactgac
    18361 aaatgcagaa tttgttgttg ctatccttga ctttgagcat caaacaatcg atccaggaac
    18421 ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg atgatcattg tacaagaaga
    18481 gacggtacct acgaacttga tgattacaca aagtctccat acgtatttaa aatcagacca
    18541 agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa acttacgtag gcgagtactt
    18601 atagaagagg aagggttgta tgtcagtgat tttgatttca ggaacgattg gtgcaggtaa
    18661 gagcagtttg acggatatgc ttgcaaaaga gattgattct aaaccatttt atgaaaatgt
    18721 cgaagataac gaagtattgc cgctctttta ttcaaatcct gagcagtacg cttttttatt
    18781 gcaaatcttc tttttgaaca agcgattttt agcaatgaaa aatgctttag tgaacgatga
    18841 caatgtgcta gaccgatcca tctatgaaga tagcctgttg ttccatctaa acgctgattt
    18901 aggtcgggta acggatatcg aagtacagca gtacgatagc ttattagata cgatgctaaa
    18961 cgagttagat gatgttgctc caaaaaaacg gcctgatttg atggtccata tcaaagtttc
    19021 tttggacacg atgctagaac gtatcaaaaa acgtgggcgt gactatgaac agctagaaag
    19081 cgatgagacg ttatatacct attatgaaac gttgaacacc cgctataatc aatggtatga
    19141 agattttgat gtatgtccta aaatacaagt ggatggggat aaattcgatt ttgtagaaaa
    19201 tccagaagat gcaaaaaagg tgatccgtca gatccaagaa aaattagctg aattggaagg
    19261 aaagcctgct ggaaaatatt ttacggataa ccagcatgca aaaggtcaag aattaggcta
    19321 aaaataaaaa accaaacgac gggaaagaaa aattactacg tgatgtagaa taagcctttc
    19381 tatcgtttgg ttattttata ttcaaatatt tgaaataaat aaatctttac tctttgattt
    19441 gtaaactgaa atgatccact gtttgtttta ctgatgaagc ttgatcaaag gaagaagctg
    19501 agtttgaagt tcataaaaat cagccatagt gagttttgct tggatttgtt tatcttcttc
    19561 tactttttac ataattttga acaggataag attcaaatgt tttaaatgaa tctttgacta
    19621 gtttgtgaca gttgtcgcat atgctcaagc ttccttggtc attaaattct gtatggatat
    19681 ttttgttgtt attacatgta caataatcag gactatcgat catccatttc gtctccttta
    19741 caatcatctt gcttctttac tatagcagaa aagaagggga gaaacaaaag aagatccaac
    19801 agacggcact atagactgta gatgttgcca tcctcatcct tcaaacggta attaccgatt
    19861 tttgtaaata tgactgttgt ttctttttta ttttcttttc ctgaaataat atattcgtca
    19921 tctttggtag tgatcttgat atctgtcagc actgtttttt cttcagatga ttcagaagaa
    19981 tcagttcctt gtgtctgata cgtgcgtgtc cattccattt ttttatctgt aaatgtcaac
    20041 tgtcccgcgg ataaatccat atctttacgt acttttagcg tatatacact gttggtggta
    20101 tctgcttttt ccgcttttaa gacagaagaa tcggttgttt cgtccgagga ttggtcagag
    20161 ccgccaaaca aatcacagcc agtcaatgtc aaaagtcccg cagacaaaat aaatagtacg
    20221 attaatttct tcaaaatact tcccccattt gataggataa agaaattata ccattggaat
    20281 gtaaaacttc aaacggatgg aagagaaaaa cgcacaaaac acccacaaat agagtaaatt
    20341 ttagaataaa attttaaaaa ggcaaaaaat ccgccacttt cagaaagcgt tttaaaaaag
    20401 tgttgcaatc ttttaaaaaa atgctatatt aaaaatgaac cggttcaaat acttgtttaa
    20461 gtataattaa tagtgagagg aagttgctct actgaagact ttagttatat ggatattgat
    20521 gacaagcaac actatgcaaa gaatgcggtc atcataatcg aacaacaaga aagagaaatc
    20581 aaatgtaact ggcaggagtg agaatgtatg gcatcgatcc gtgatgtggc taagatggct
    20641 ggtgtttcag tgggaacagt ttcccgttac ttgaatggtc agcaactgaa ggaaaagaac
    20701 atgaaaaaaa tagcagaagc gatttcagcg ttggactata aagaaaacat cattgctaaa
    20761 gggttgaaga ataatcgaag cttctctatt ggacttttga taaacgggat ttccagccgt
    20821 tttgggtcgg aagttgtctc agggatcgaa agagtagctg aagaaaatgg gtatagctta
    20881 ttattaagcg ggtttgccga tcagccagaa aaaattgaac aaaaaattga atacctgatg
    20941 aagcatgtga tcgatgggct gattgtcttc ttatccgaag aagagtggtc tggatttgaa
    21001 atgctcacaa aaatgagtat tccagttatc gcattgaatt gtccagaagg accagcaggt
    21061 attgatacaa tattagtgaa tgatcgagaa agtgtagcaa aagtgatcgc tcatcatggt
    21121 gaacaaggac ataaaaaaat cggatttatt gctgccactc aaacagacta cgtagcaaga
    21181 gaacgtttag caggagccaa agaagcggtc caaaatgatc catcgatcca attagaggtc
    21241 tttacaggag actattctag aaaaagcggc tattacgggg caaaagcatt attagaaaaa
    21301 ggggcaactg ctatttttgt cagcaattac aatatgtcga ttggtgccat cgaatgtttc
    21361 aatcaagaag gtgtcagaat cgggaaggat attgcgttca gtcactatga ttatttagat
    21421 aaaaacgatc tgagtatcct tccgaaaatc acgatcacgc caccaacaga taaaatcggg
    21481 gaacgagcag cagaacagct gttaaaacga atcaaaaaag aagaggaaga aaatgaaaaa
    21541 gtgattgtga tggaaaatat catacatgga atcaactaaa acgaactaaa aaagaaaaag
    21601 aggtgctttg atgaaaaaat atcaatttcc agcaaatttt tggtggggtt ctgcagcaag
    21661 tggcccacaa acagaaggaa ggatttcagg cgatggaaaa ggagagaaca tttttgatta
    21721 ttggtacaaa aaagaaccgc aaaagttctt tgatcaagta ggacctgaaa aaacctcgca
    21781 agtttatacg aagtatcaag aggatgtaca gttgatgaaa cagactgggc ataattcatt
    21841 tcgaacatct atccaatgga gccgtttgat acctgatgaa tctggaacaa tcaacgaaga
    21901 agccgttcgt ttctatcatc gttattttga tgagttgatt gaaaacggta tcgaaccctt
    21961 tgtgaatctg tatcatttcg atatgccgat gtatctgcaa gaaaaaggcg gatggttgaa
    22021 ccgagaaacg gttgaagcct atgaaaaata tgccaaaagt tgttttgaat tgtttggttc
    22081 gaaagtaaaa aaatggttta ctcataatga accaatcgtt ccagtcgaag caggttattt
    22141 atatcgatgg cattatccag aagagtcaga tatgaaaaaa gcgatccaag ttggctatca
    22201 tgaagcgttg gcttcagcgt tagccatcaa atcttaccat gaaatggagc aaggtggtgt
    22261 tattggtatc atcttgaatc tgacaccaag ctatccaaga gacgaatcaa accctgaaga
    22321 tgttaaagca gcgcaaatag cagatgcgtt cttcaatcgt tcattccttg atccagctgt
    22381 aaaaggcgag tttccaagtg aattgattga gatagtaaaa gaacttgatc tcatgcctgt
    22441 gatccatgca gaggatttga cggttatcaa agaaaataca gtagatcttt taggtatcaa
    22501 ttattaccag ccacgccggg taaaagcaaa agaaacacca atcgatacta aaaacggtcc
    22561 aatgcctgag gattatttcg acaattatga aatgcctggt cgtaaaatga atccttatcg
    22621 cggatgggag atctatgaac aaggaatcta tgatattttg atcaatgtac gagatcatta
    22681 tggaaatatc gactgcttta tttctgaaaa cggtatgggt gtagaaaacg aagcacgatt
    22741 tatcaaagaa gatggcatga tccacgatga ttatcggatc gaatttgtcc aatctcatct
    22801 gaaatatgtc catcaagcca ttcaagaagg tgccaattgt ttaggctatc atatgtggac
    22861 atgtatggat aactggtcgt ggaccaatgc atataagaat cgatatggct ttatttcagt
    22921 agacttagca caagatggaa aaagaacaat caaaaaatcg ggtcattggt tcaaagacgt
    22981 atcagataaa aacggatttg aggcttaaga aagaatagtt gagagcaaat gcgtgaaatt
    23041 ctccattctt accattttgt gaaaaagaag ttcaattatt tacgatcttt ttcaactttt
    23101 ttgtctttct atttcatttt ttgtatataa tagaatagtc aaaattaaat aggagtggag
    23161 gaaagcatga ggaaaaaaag accgtttctt ctcttttggt tattcggttt tttcttactg
    23221 cttattttat ccgcagcatt ttcaggaaaa atgacagcag atcttgctca gaaagaaaca
    23281 gaaaaaatcg tagcaagcaa agaatcagta gataatttca aaaaagaatt ctcaacttat
    23341 tggtgggcgg agtataaaat aactgccaat tacgaaaaag gcgaattgga catttatttg
    23401 ccaaaaggaa aagaagaaaa tcaatttatt aatatcgatg acgtacttag tgcgatttct
    23461 ctcagacaat atcaatttaa agacaagcat ttgaccgttt tgcttttgac tcatgatggc
    23521 gacaaagtag cccgtttgat caccgcttat tctgaaaaag tagacaaaaa agtggtcgcc
    23581 gatcgtttag cggaatggaa aaagacaaat gaacaagtca agaaaacaac tgtctcttct
    23641 caaaaaaacg agtcgacagg aacaagtcct tcagattctg gcagtggaca aacgataacg
    23701 ggaagtgaag aaaatcaagc ctcaattatt acgaatgagt catcacaagg acaatatcaa
    23761 tcctatggac aatgaaaaac agaggttgtg aaatcagcgt tt
//
LOCUS       DACETN010000042        23168 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DACETN010000042 DACETN010000000
VERSION     DACETN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.043"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            363..731
                     /locus_tag="IXF98_001487"
     CDS             363..731
                     /locus_tag="IXF98_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1555806.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IXF98_001488"
     CDS             911..1852
                     /locus_tag="IXF98_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1555807.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IXF98_001489"
     CDS             1921..2652
                     /locus_tag="IXF98_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1555808.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IXF98_001490"
     CDS             2796..4976
                     /locus_tag="IXF98_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1555809.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IXF98_001491"
     CDS             4996..5478
                     /locus_tag="IXF98_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1555810.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IXF98_001492"
     CDS             5494..6255
                     /locus_tag="IXF98_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1555811.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IXF98_001493"
     CDS             6386..7720
                     /locus_tag="IXF98_001493"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1555812.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IXF98_001494"
     CDS             8027..8512
                     /locus_tag="IXF98_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1555813.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IXF98_001495"
     CDS             8505..9188
                     /locus_tag="IXF98_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1555814.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IXF98_001496"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IXF98_001496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1555815.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IXF98_001497"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IXF98_001497"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1555816.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IXF98_001498"
     CDS             12804..13433
                     /locus_tag="IXF98_001498"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1555817.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IXF98_001499"
     CDS             13485..13817
                     /locus_tag="IXF98_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1555818.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IXF98_001500"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IXF98_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1555819.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IXF98_001501"
     CDS             14643..16016
                     /locus_tag="IXF98_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1555820.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IXF98_001502"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IXF98_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1555821.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IXF98_001503"
     CDS             17071..17511
                     /locus_tag="IXF98_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1555822.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IXF98_001504"
     CDS             17655..18356
                     /locus_tag="IXF98_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1555823.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IXF98_001505"
     CDS             18353..19474
                     /locus_tag="IXF98_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1555824.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IXF98_001506"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IXF98_001506"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1555825.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IXF98_001507"
     CDS             20853..21761
                     /locus_tag="IXF98_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1555826.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IXF98_001508"
     CDS             21796..22068
                     /locus_tag="IXF98_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1555827.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IXF98_001509"
     CDS             22079..23125
                     /locus_tag="IXF98_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1555828.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7807 a         4039 c         5077 g         6245 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaac
//
LOCUS       DACETN010000043        21659 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DACETN010000043 DACETN010000000
VERSION     DACETN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.044"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(408..1904)
                     /gene="lysS"
                     /locus_tag="IXF98_001510"
     CDS             complement(408..1904)
                     /gene="lysS"
                     /locus_tag="IXF98_001510"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1555829.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2015..3019)
                     /gene="dusB"
                     /locus_tag="IXF98_001511"
     CDS             complement(2015..3019)
                     /gene="dusB"
                     /locus_tag="IXF98_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1555830.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3020..3907)
                     /gene="hslO"
                     /locus_tag="IXF98_001512"
     CDS             complement(3020..3907)
                     /gene="hslO"
                     /locus_tag="IXF98_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1555831.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4124..6235)
                     /gene="hflB"
                     /locus_tag="IXF98_001513"
     CDS             complement(4124..6235)
                     /gene="hflB"
                     /locus_tag="IXF98_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1555832.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6325..6870)
                     /gene="hpt"
                     /locus_tag="IXF98_001514"
     CDS             complement(6325..6870)
                     /gene="hpt"
                     /locus_tag="IXF98_001514"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1555833.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6870..8315)
                     /gene="tilS"
                     /locus_tag="IXF98_001515"
     CDS             complement(6870..8315)
                     /gene="tilS"
                     /locus_tag="IXF98_001515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1555834.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8435..8905)
                     /locus_tag="IXF98_001516"
     CDS             complement(8435..8905)
                     /locus_tag="IXF98_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1555835.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8951..9376)
                     /locus_tag="IXF98_001517"
     CDS             complement(8951..9376)
                     /locus_tag="IXF98_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1555836.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9443..9712)
                     /locus_tag="IXF98_001518"
     CDS             complement(9443..9712)
                     /locus_tag="IXF98_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1555837.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9723..11318)
                     /locus_tag="IXF98_001519"
     CDS             complement(9723..11318)
                     /locus_tag="IXF98_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1555838.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11333..14854)
                     /gene="mfd"
                     /locus_tag="IXF98_001520"
     CDS             complement(11333..14854)
                     /gene="mfd"
                     /locus_tag="IXF98_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1555839.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14871..15431)
                     /locus_tag="IXF98_001521"
     CDS             complement(14871..15431)
                     /locus_tag="IXF98_001521"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1555840.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15784..16758
                     /locus_tag="IXF98_001522"
     CDS             15784..16758
                     /locus_tag="IXF98_001522"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1555841.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16928..18328
                     /locus_tag="IXF98_001523"
     CDS             16928..18328
                     /locus_tag="IXF98_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1555842.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18406..19128)
                     /locus_tag="IXF98_001524"
     CDS             complement(18406..19128)
                     /locus_tag="IXF98_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1555843.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19207..19713)
                     /locus_tag="IXF98_001525"
     CDS             complement(19207..19713)
                     /locus_tag="IXF98_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1555844.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19836..21635
                     /locus_tag="IXF98_001526"
     CDS             19836..21635
                     /locus_tag="IXF98_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1555845.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6014 a         4725 c         3883 g         7037 t
ORIGIN      
        1 ctcatttatc ataacttatc gtttgttatt tgtcaagaac ttttttgaag tttttttcga
       61 tagaatcaag cttcgcaagt ggttcttttt taacgactca gttatcttat catgtcgtta
      121 gtaacatgtc aataactttt ttgttatgtt tttgaataaa tattttcttc tgtcttttgg
      181 acaagtaata atataccaac taaatttagc ttggtcaacg aattttttgt attttttcaa
      241 gtttttttca ataaacgatt attcataact tttctttctt tatcgttcgt tttcgtcttc
      301 tttaatgtct agaatagtcg gcattagctt ttgaactttc gttattattt attcctttct
      361 acacagcaaa agctgcctaa ggaattcctt agacagcttt tattcgatta tctcattgtt
      421 gggaatagca aaacatcccg aattgattgt gcatcagtta gcaacattac taatctgtcg
      481 atcccgatgc ctagaccacc agttggcggc ataccgtatt ctaaggcttc caagaagtct
      541 tcgtctacac cgtgagcttc gtcatttcct agttcacgtt ctttttcttg cgcttcgaat
      601 ctttctcttt gatcaattgg atcgttcagt tctgtaaagg cattagcaaa ttcgcgcccc
      661 actatgaata gttcgaatcg atctgtgaat cgaggatctt ctggattttt cttcgctaaa
      721 ggcgaaactt cgactggatg tccgtataca aacgtcggtt gtgtcaatgt ttcttcaaca
      781 aatgtttcaa agaattcatt gataacatgt ccgacaccca tagcttcggt tacttctaca
      841 ttgtgttttt cggctaaagc taatgcttct tccaatgtca tttcttgcca gaagtctaca
      901 ccagtttgtt ctttgattgc atctaccata tggatgcgtt taaagtcact ttctaagtcg
      961 acagcttgtc catcatatgt gatttttgct gttcctagta ctttttcagc tgcgttacgg
     1021 ataatacctt ctgtcagatt cattacatca ttgaaatcag tatatgctgt gtatgcttct
     1081 aacatcgtaa attctggatt atgtgtagta tcaattcctt cattacggaa aacgcggccg
     1141 atttcataaa ctttttccat tccgccaaca attagtcgtt ttaaatgaag ttctagcgca
     1201 atacgcaaat ataaatcaat gtccagtgca ttatgatgcg tgataaatgg gcgtgcggca
     1261 gctccacctg cctcattgtg caatactggt gtttctactt caatatagcc attgccatct
     1321 aaatagcgac ggatttcgct gataatttgg cttcgtttca taaatcggtc aaagctttcg
     1381 cgattactga ttaaatccaa gtagcgttgt ctgtagcgtt gttcaatatt cgttaagcca
     1441 tggtatttat ctggcagtgg acgaagtgct tttgacaaaa tcgtgatttc tgaagctttg
     1501 atagaaactt cgcctgtgtc tgttttcatg atttgtccag ttacaccaaa gaaatctcca
     1561 aggtctgcat gtttgaatac ttcataggcc tcatcaccta cttggtcttt tcttacataa
     1621 atttgaattt ggccttcacg atcttgtaga tgcgcaaatc cagctttccc ttttcctcgg
     1681 cgagtcatca tacgaccagc tacactagcg gtcaatccca tttctgctag ttcttctttt
     1741 gttcttggat caaatagttc atgcaattct tctgagttgt gcgtgcgttc aaatcgtttt
     1801 ccaaatggat cgattccatc ttcacgcaat tgttccattt tttgacgacg tacaagcatt
     1861 tgatcattta aatcttctac ttgatgttgt tgttcctgtg tcacaaaagt tcctccattc
     1921 tcatgttaat ctctttccta taatgccaat gaaacacaaa aaaagcaagc cgaacttgga
     1981 atttcggctt gaaaattcac gaaaaactta gaaatcacaa cgtttgtttc aatagtactt
     2041 tttccgcttt ttctacaaat tgatccagca ggtcattgat ttcttgctgt ttttctgtct
     2101 gattgattgc tactttcact ttcgctgctc gaggaatccc ttttaaataa taagccgcat
     2161 gtgttctgaa ttctctacaa gctacttttt ctcctttgag atctgctaag cgttgtaaat
     2221 gaagtttagc tgtagcaatt ttttcggctg gtgttggttc aggaatcagt tctcctgttt
     2281 cgagataatg ctgtgtgcgg tgaatcatcc atggattgcc cagcgctgca cgaccaatca
     2341 tcacaccgtc tgcaccgaca tagtcaagca tccattttgc atcttctggc gttttgacat
     2401 ccccgttccc cataaatggg atcgtcaaat gttttttcac ttctttcaat acttcccagt
     2461 ccgcttttcc ttcatacatc tgaacccgtg tacgtccatg catagcgatg gcacttgctc
     2521 cagctgattg tgctgctaat gcattttcta cagcaaagac gtgctcttca tcccaaccaa
     2581 tacgcatctt gattgttact gggatattta ctgctgaaga aacagcttgg accatttcgt
     2641 atactttctc aggatctaaa agccattttg ctcctgcttc tgctttgatt actttattta
     2701 ctggacagcc catattgata tctattatat cggcagctgt gttttcttcg acaaattgag
     2761 cagcttcaac tagactatct ttatttccgc caaatatctg taaacttaat ggatgttctg
     2821 tttcttcaat gtggagcatc tctaaagttt ttttgtttct gaaatgaata ccttggtcac
     2881 tgatcatttc acatacgact aagcccgcac caaattcttt gacagtgact cgaaatgctg
     2941 aatttgttat tccagccatc ggtgcaacaa cgacacgatt agggatttcc acattgccaa
     3001 ttttccatgt gtgttccact taaatccctc ctaaggcttc tttcttcaat ccatctaatt
     3061 cttcttcgct atacttgtat ttgttattac agaaagaaca aaccgcttct gcaccgtgat
     3121 cttcgtctat cattgcttgg atttcttctg cacctaacgc gataattgct tctgcaaatt
     3181 tttcttttga acagtcacat ttgaattgaa caggcatttt ttccaaaatg ttcaattctt
     3241 ctgtaccaag caatcctttc aatatttctt ctggtgtctg tccttcatct aagagggtag
     3301 agattcttgg tgtcgttttt aattgttctt cgatcttgct gattgtttct tcatcggcac
     3361 ctggcatgat ttggatcatg aatcctcccg ccgtcttgat gctgtcgtca gtattgacca
     3421 atacactcag tccaacagct gaagggattt gttctgagat agcaagatag taagtaaaat
     3481 cttcagcgat ttctccagaa acaatcggcg tttgcccaga aaaaggttct tttaatccta
     3541 agtcttttgt gacagtaaac attccttgtg ttccaactgc tccccgcaca tcgattttac
     3601 cttcactatt tagaggaaga ctgagatgcg gatttttgat gtaacctttc acatctccct
     3661 ttccatttgc atctactaca attcctgttg ctggaccatc gccttggatt cgaacagtta
     3721 gtttgtcttc tcctttgagc gtcgcaccaa gtagcagact accgaccaat gtccgtccca
     3781 aagcagctga ggaggtgtgc caagtatcat gacgtctttg tgcctccgct acggtatttg
     3841 ttgcttgaac ggcgtatgct cggacgaatc cgtcgtgagc aagtgcctta accaaataat
     3901 ctttcatttg ttatgtactc ctttttccgt ttttaaactc atagtgaatc atactagcaa
     3961 ctactgcttt tttcaacttt ggaatgcttc ttttaaaaat aattcagaat catctagaaa
     4021 acaggacatc accctattta caacttaaat aaagaaatct cccgaaaacc ggtttttgtt
     4081 ccggttttcg ggagtcatat cagatcaatt gacctatatt tttttatttg tattgatcgt
     4141 ctgaatgatt atcttctgat tgttgagaat ctgaatcatg ttctgcttta ttttcctcat
     4201 tcagttcatg ttttgcttct tcaaaatcac gtttttcttc cgcttgtttt tcagcgtctt
     4261 tttcttccaa agcacgtttt gcttcttcaa aagttttcgc ttctttttcg cttggataat
     4321 catttgactc agtaccttca ggcatcacac cgtgttcaaa caatgacttg attgctttgg
     4381 catccaatgt ttcgaattcc aaaagttttt cagcaatcag tttatgctgt gcacgatgag
     4441 cttcgatgat ttctcgagct ttatcatgag cttccatcaa gatttttcgt acttcttgat
     4501 caatctcgaa agcgacttgt tccgagtaag cttttgtttg accatagtcg cggccaacaa
     4561 acacttgatg gtttccttca tattgaacag gacctaaacg atcactcatt ccatattctg
     4621 ttaccatgct tcgtgctaga gctgtcgctt gctcaaagtc attggaagca ccagttgatt
     4681 ggacattgaa gatgatttct tcagctgtac gtccaccaag taagcctacg atttgttcaa
     4741 acatatcttc tttcgtcatc aagaactgat cttcttttgg taaagcgatc atatatccgc
     4801 cggcgcgtcc gcgagggatg atcgttactt tgtggacgac ccgagcgcga ctcaatacta
     4861 atccgacaat cgtatgacca gcttcgtgat aagcaaccat ttcgcgttct tttttgctga
     4921 ttacgcgatc ttttttagct ggacctgcaa tcacgcgatc ttccgcttca tcgatatcag
     4981 aagcatcgat tttcttttta tttcttctag cagctactaa cgctgcttca ttcaagacat
     5041 tttctaaatc tgctccggca aaacctggtg tctgttgagc cacaactttc aagtcaacgt
     5101 catctgccat cggtttattc cgtgcatgga ctcgcaagat tgcttcacga ccttttacat
     5161 ctggacgtcc aactaaaatc tggcggtcaa aacggcctgg acgtagtaac gctgggtcta
     5221 gtacatcaga acggttggtt gcagcaatga cgattactcc ttcatttccg tcgaagccat
     5281 ccatttctac aagcaattgg ttcaatgttt gttcgcgttc atcatgtccg ccgcccatac
     5341 cggcaccacg ttgacgacct accgcatcaa tttcatcaat gaaaatgata gctggtgcgt
     5401 ttttcttcgc tgtttcaaac aaatcacgaa cacgacttgc tccaacaccg acaaacattt
     5461 ctacgaaatc agaacccgag atcgagtaaa acggtactcc agcttctccg gcaacagctt
     5521 ttgctaataa ggttttacct gttcctggag gtccttctaa aagtacacct gccgggatac
     5581 gggcacctaa ttcaacaaat cggcgaggat ctttcagaaa ttcaacaact tcaactagtt
     5641 cttgtttttc ttcttccgca cccgctacat cagaaaaacg tacgcgatta gcttttttgt
     5701 ctgcttcttt ggcttttgat ttaccaaagt tcatgacacg gccgccaccg ccaccgccgc
     5761 cgccttgttg tcccatcatc atgtaaaaga aaaagatgat gattacgatt ggcaagaagc
     5821 taagcaatag ggaaacccat ataccgctgc ttgattcttc ttttactgtt gtagcaactt
     5881 tgttttcttt tgccagttct gttacttggc tcaatgttgt atcatttggc aaaatgattg
     5941 ttgtgaaatg ggtagtagtc gtatctgttg aacctaagat agctaaccca ccgctgttag
     6001 cgacggtctg ctgttctttg tattcaccag tgatcttata tacaccattt gcaggctgca
     6061 cttcaaattc tttgatcttg cctgcttgta gttgttctgt gaattttgag tactcgatat
     6121 ctgacgatcg ctggccgtta ttaccaaaaa tgaaataaac gaccattacc atcgctaaga
     6181 ttacgaggac ataatacaag gcatttttca tcatgccgtt gttctttttg ttcatagttg
     6241 ccctccctaa gcgtcaattt aatttaacag ttttttatca atcctgacca ttctattgta
     6301 ccataattta attgagatac agcattaatt tgattgataa acactaggtt tcaatacacc
     6361 gatgtacgga aggttacgat atgcttctgc ataatccaag ccataaccga ccacaaattc
     6421 atttggtacg ttgaagccaa cataatctgc ttcgatattt actactcttc cttcaggttt
     6481 gtcaagtagt gtaacaattt tgactgattt tgctttgcgg tatttgaaaa gatccactaa
     6541 ataagcaagt gttcttccgc tgtcaatgat atcttcaacg atcaacagat cgcgaccttc
     6601 tacattcgta tctaagtctt tgacgatttt tacttcccct gacgagactg ttgcattccc
     6661 gtagcttgaa acatccatga aatccaattc caaatacgta tcgatagaac gaacgatatc
     6721 tgccatgaat ggtaccgctc cttttaagac acctactact aggggattgg tgttttggta
     6781 aatttctgtt aactctttgc ctagttcttt gcaacgaact tgtatctctt cttgtgagat
     6841 caaaattctc tcaatatcct tttccaacat caggttctcc ttccaattgc ttctttttga
     6901 tatttgtaaa gaagtatgta gtgtatttta tcagtttctt cacgaatact caaatatgaa
     6961 aaggcaaaag gtatcaagct atatatattt cttttggcat ctgtaatgat ccaagcatgc
     7021 tgcctttgtg aattaggtac tttctgatcg ataaaatatc tagatacttt ttttgtgaga
     7081 ttctctgtta atttaatgcg gtctcctgct tttctttttt cgacatacag ttgcacatca
     7141 gcaggaatcc aaatgtcatg agaaaataga gaccatgaat ctgttgcttt gatgtcccct
     7201 gcattttcag gtttgaccag accaatccat tctgtttcat tcaaatatat tccttgttct
     7261 ggaatcagca acaagcgctg gtcttctgtc tggctagttg cttgtttttc ttttttagag
     7321 agtacaacaa aatgatactc tcttttcagt atccaattat tgccaatact gagttgccat
     7381 tggctttttt tctgttgcca ctggtctaaa atcgactcca tttgttgttc tttcaaaata
     7441 attcctgttt ttgagaatgc tttgtcgaaa aaggagtaaa aaaaatagta tcgttcagca
     7501 tctgtcattt cttcaatcat ttctgcagaa aatcgaaatt gttcctccca ctgttcgact
     7561 tccttttcga tcaactgtcc catatatttt tgaatgactt ggtctgccca ttggatctgt
     7621 tgcgaaaatt gctggaaatg tctcaacgcc tgtgtatttt cttgtttcaa ttgaggaact
     7681 accaaatgac gaagccgatt tctctggata tctaacaatt ggtttgtctg atcctcaaag
     7741 taaacaagct gcgattccgc tgcataagta tataggtctt ctttgctaaa actcagcaac
     7801 ggacgaatca gtctgcccgt agcaaatggt tgtacttctt tgattccaga aaaagtattt
     7861 aactgtcccc cacgaacgat tttcattaac accgtctcga tttgatcatc tccgtgatga
     7921 gcagtcatca acgtgtcata ttgtccttct tccatcacgc gagtgaattc ttgataacga
     7981 aagttacgag cttctgtttc tatggcggat ttggctggat cttcccaaat acttacatat
     8041 aaagggagat tcttctcttc acaatatgag cgaagatagg cagcttcttc tgctgattct
     8101 tctcttaatt ggtgattgat gtggacgata cctaaagaaa aaccgatttg gcgttgcact
     8161 tgtaaaagta aatggagcaa aaccatcgaa tcaacaccac ctgataccgc tgctaaaatt
     8221 ctcgatcctt gttgccagaa tccttgttca ttcccgtgcc gaataaatga ctgctgtaat
     8281 agcggtgctt ttttcccgcc tttttcaacg ttcatccttt ccacaccctt tcttcctaaa
     8341 aaaaagagag cgggaaaacg tctaaaacga caattaacct gccctctgtt gatactatga
     8401 cccaaatagt cgcactatca ttatttgcgg ctttttagct gcgacgtccg ccacggccac
     8461 cgcgctttcc ttctgtgttt cgcttcaatg aagataagcg gtcatcgcta tctttcaaaa
     8521 atgaactcat caatgaatcg aagtcttctt tttgagctga aggttgactg cgagaaaaag
     8581 attttttctg tgctggacga tttcggttgt cacggggatc tttttgttga tattttttag
     8641 gacggctttc ttctccagat tcagcaggtt gttcctgcgc tttccggata gatagtccga
     8701 tttttccatc atcatttact gaaataactt taactgttac ttcatctcct acacttaaaa
     8761 catcatggat gtcttttaca tagccatttg acacttcgct aatatgaact agacccgttt
     8821 ttccagcacc tagatcaata aacgcaccaa aatttgtgat tcctgatact tttccttgca
     8881 gttttgctcc tacctcgatt gacataaaaa aaatgttcct cctaattatt tccctttttt
     8941 atattcctgc ttattttgca gttgtagaac tagttgattg atctgtttca tttgtcgagc
     9001 tgtctgtatt tgattctaat ataggataaa ctttttcacc gtcttttgag taaaagaatt
     9061 tgcttctggc aagtttagct acatactcat catctttcag caatgaaaca tcttggtgca
     9121 gctgatcaac gtctgcgttg acttctttca tttcagtatt tgcttcgact ctcaattcgt
     9181 tcaattgatg catcctttgc atgtcatgga aaatctgcac acctattatc gcgaaaatga
     9241 ccaaagcacc aacaaaaatt gcagccagtc tgcgtcttcg aaaaatcagt tgacgttgtt
     9301 gttgctgaaa ttcagcatac tttttctttg catattccgt atctagcgca gcgattttat
     9361 tgcttttttt cgtcacttgg attcgctcca atctttttga ttgtcttttt cattatacca
     9421 atatcgtgaa agcttgtcta atctatttct ctagtgtttc tgtgcgattt tcagaaataa
     9481 tttcgtacat tttttgtgca tcttcttttt tggttgattc atgtaattcc gtaattttca
     9541 cttccaacgt tttgttgcca aactggactt tcaaaatatc gcctactttg acatcactgg
     9601 aagatttcgc caatttaccg ttgatttgaa ttcttccttt atcagctact tcttttgcta
     9661 ctgatcgtct tttgatgatt cgggaaattt ttaaaaattt atctaatcgc atatcttatt
     9721 cctcactttt taattttttt atttaatcgc aggattttct taccaaaagg caacattagc
     9781 cattctcgta ctgtaaacag acgaagacga atcactgcat aaatgaacac agaagccccg
     9841 atcccaactc ctatcacgct gacaaaaaaa gcagtggaac gatgagttac aggaccaaat
     9901 actatattca atacaccata atacagcagc agtactactg tcatacaaag aaggctgaac
     9961 atcaacttcc gtccaaaatg ccgttctttc caaaaagcat ttagctcttt tgtttcaatt
    10021 cgaacaaaat accaaagcgt cacacctaat cctaaaaggg tagacaggct tgcacctgct
    10081 gttcctaata aaccggtcag aaaagaagta gtcaatcctt ttaccaatag tccccatcct
    10141 gcgcctttta aagaagggcg aaacgagttc ttgctttgag caatactttg ataagcttgg
    10201 atgactgcag tgaaagcaat ggaaaaaaca aataatacaa gcgctgcatt tcccgcataa
    10261 tctttaaaca acgcataatt gatataaggg agcagcaagg ctaatcccaa agacgcagct
    10321 aatgctaaag cggtcgtcaa tctaagatag attttggctg tctgtaaaaa ctgacgatag
    10381 atccggttgg tcaaatgacg agtcaacgca ggcaggaatg tcgcacttaa tgcagtggca
    10441 atcactaaac ctaattgtac taaaggctga cctcgatcat agaccccttt tgctatcttc
    10501 gcaccgtatt ctgacagtcc gtaaacttcc aaagcatttt ttacaaagaa agagtcgatc
    10561 agctggaaaa agataagcaa tccactataa atcgaaacca gtccgccttc taccagaaaa
    10621 cgccggatca agggctttga cggctgtttg aacaatggaa accgtcggaa gcttaacgct
    10681 cctccatgaa tcttttgttc atagtagtga aggatcccat atgcgcataa accgccaaaa
    10741 acagctccac tcattgctgc cgtccctgtt tggtacaccg tccatccaag ctttctaaaa
    10801 gcaaacgcac tacagacaat cacaaaaaca cgaacgaact gctcaactac ttgagaaaca
    10861 gcagttggtt ccatcaagaa tcttccttgg aaattccctc gataaaaaga tagaccaggc
    10921 atcagcaaaa aggtaaagga aactacttgg atcagcggtt tcaattgtat gtctcccatc
    10981 atccaagcaa tccagctgct aaatgcaaag acaaacaacc acaaaaagac acctgtccaa
    11041 aatactaagg gataaagatt ttgcaaagct tgctttcgct cgaaaggatt ctttctttct
    11101 gcaacgtact ttgatatgaa ctgcggaaga ccggataaag ctaaagtcat tgctatccca
    11161 taaattggat aaacttgctg ataaacataa aaaccttcgt cgccgactaa gttttggagg
    11221 ggaattcggt aaagggcact taatactttt gctatgaaag aagcaatcgt caatacaaat
    11281 gctccttgca tgacgcgccg catttcttta tgatccaaat cattgctcct tcctacgctg
    11341 acagatattt ttgctcacgc aacgctttga caaacttttg gatttcctga agccatactg
    11401 cttcttccat ttggttgggg atcgtcaagc gtatcaccat ctgttctttt tcaacgccta
    11461 gactggcttt tagttttgtc gctgtcagtg cttcgaataa ctgttccaca ctatagcgtt
    11521 ttgttccaac tttacttaga gtaaagacta tatgcgcctc ctgtttcctt atcgtttcta
    11581 ggagcgctcg atcgccatcc attttgatct gcccaattgt caagagatgt gctacttctt
    11641 ctggatattc tccaaaacgg tcgagcagat catcttctag ttcatctagc atttcttgcg
    11701 aatcaagttc acgaattcgt ttatagatct cgattttctg ccgttgatct gccacgtatg
    11761 tttcaggtag atacgcatcg acaccgaggt cgatctcaac agtggtcttc tccacttgac
    11821 tattctttcc ttgtttacga gaaacagctt cgcttagcat ttgagaatac atatcgaaac
    11881 cgactgcatc aataaatcca tgctgctgag cacctaagag attacctgca ccgcgaatgg
    11941 aaagatcccg catcgcaatc ttgaatccag atcccagttc tgtaaaatct ttgattgcct
    12001 gaagacgttt ttcactgact tcgttaagga tcttctgctg ctcatacata aagtaagcat
    12061 atgcgacacg gctgcttctt cctacccgcc ctcgcaattg gtataacgtg gataatccca
    12121 tataatcagc gttctcaacg aacaacgtat ttgcgttcgg gatatctact cctgtttcaa
    12181 tgatggttgt agtgacaaga acatcatatt gaccttcaat aaattcaaaa agcgtatttt
    12241 ctaattgcgc ctctgtcatt tgcccatgag cataaccgat ccgtgcttcc ggaacgagtt
    12301 cttggatttc ttctactttt tgttcgatcg tttctacccg attatataaa tagaatactt
    12361 gtccacctcg tcccatttct cgatggattg cttcgcgaat agccccagga tttttttcca
    12421 taacatacgt ttggatcgga taacgattcg caggcggtgt ttcgattact gaaagatccc
    12481 gcacgcctaa catcgacata tgcaaagttc tcggaatcgg tgttgcagtc agagtcaaaa
    12541 catccacttg tgcacgtaac tgtttcaatc gttccttatg ttttacacca aaacgttgtt
    12601 cttcgtcaat gatcagtaga cccaaatcgc taaattcaat atcttttgac aaaattcggt
    12661 gggttcctac aacaatatca acttgacctg tacgcagttg ttcgatggtt tctttttgtt
    12721 gctttcttgt tctgaaacga cttaatagcc cgatatttac ggggaaacct tcaaatcttt
    12781 ccagcatcgt ttcatagtgc tgctgtgcca agatcgttgt tggaactaaa aaggctactt
    12841 gtttgctttc ctttatcgct ttaaaggccg ctcgaagcgc tacttctgtt ttcccataac
    12901 caacatcacc tacgagtagt cgatccattg gtttttcttt ttccatatcc cgtttgatct
    12961 ccgctgcact tctcaactgg tcatcagttt cagagtaggg gaaggcattt tcgaattctt
    13021 tttggtaggc gtcatcaggt ccaaaagcat atcctttttc tgactctctt gcggcatata
    13081 atttgattag atcgtcagcg atatcctcga tttttgaaga gacttttcgc ttggttttcg
    13141 tccattcact tccgcctagc ttattgatgc gaggcgcttt ggcttcagag gcgacatatt
    13201 tttggatcag attcagttga gtaacaggaa taaacagttt gtcgtcgttt tgatacaaaa
    13261 tagtcatata gtcttggtga acgccatcta cttctagcgt ttccatccca atgtatttcc
    13321 cgataccatg attggcatgg acaacgtaat ctcctgcttt taactcatta tagcttttca
    13381 atcgctctgc gtttgttact gtttgacgtc tcgtttgttt tttcgtcgtt ttttggaaaa
    13441 tttctttttc tgtgatagct actatttttt cttgaggaag ttcaaaacca gcctgcaaag
    13501 ctccttcaac taattgtacc tgtcctctga tcaatgcatt tttttctgtt tctactacta
    13561 atatgtcttc atctcgaagc atttgttctg ctttgcgtat tctgtccttt gtcggaataa
    13621 agatcacaac agtctgtttt tgcttgcgcc aacggtccat ttctgttttc agcaatggca
    13681 tttgtccgaa gaactgttgc atcgtccgat attgaaaatt atggattgct tggaaacgaa
    13741 gatttcccat ccctttttgg aagagtgaga aaaaggttgt ggtgaacttt tccttctgca
    13801 cgattttttg tatatctgct gaaaatgtct gctcagaaaa cacccgcaat tcgctgattt
    13861 tttgagtctg ccattctgcc gcttcccttt cgatttcccg atttgtttcc atgattcgct
    13921 gataatcatc aacgactagt aaactatttg aagggaaata atccaagatc gtcacagatt
    13981 cttggtaaag aagatctgag tagaattttg cttcttctgt cgggacacct gcttgccagc
    14041 ttgaagtcag ctgaccgaag taatcttgca aaaactcgcg gtctgcttct tctgtggtcg
    14101 cagcaagccg tttttctagc attttttgca tttgctctat tccatgttct agatcctctg
    14161 gtccgtagac ttgttcactc gttggggaaa cccagatttc ttccgttttt tcgattgagc
    14221 gttgtgtttc tgcttcaaaa taccgcatag aatctatctc tacatcaaat agttctacac
    14281 gaacaggata ggttgtcgtt aacgggtaaa tatcgataat gcttcctcga atactgaatt
    14341 ctcccggttt tgcgaccatc tcttctcgta catagcccat caaagtcaat tggcgaggaa
    14401 gattttccac gtctatctca tcaccaatat gccaattcaa ttgcgcctgt agccaagttt
    14461 tcttattcgg cagtcgtttg tgcaaagatg cgactggcaa caaatagata cctgcttttt
    14521 tttcttgaag tgcgttaagc gttgcaactc tctcgcctcg cgcttctgga gaagaaaagg
    14581 ccatttcagc agaaagcact tcatccactg gaaataaatg gatctgatct tgcggaatca
    14641 catgttgcag atcttctgct aactgattcg cataatacaa atttggcatc agaataatca
    14701 tgtttttatt tgttttctta aacgctcgag caaatagaag tgttttcgct gagcccgcta
    14761 atcctgtgat tagttgtcta ggcatttttt cttctaaatt gctgacccat tcttttactt
    14821 gcggatcttt tccgactaat tgaataatat ccattccttt tcctccttcc tcaattgaat
    14881 cgattcatcg catccacgaa cgaatgccct tctatgagat attcgactgc tttaacagat
    14941 tgacacatgc tttcttctat caaaggctga ttttctttgc tgaaaggact aagcacatgc
    15001 tggactactg tttttttccc ttctggcctg ccgattccca ccttgattcg atcaaagaca
    15061 ttcgtattca aatgggaaat aatacttttg ataccgttat gcccgccggc acttcccttt
    15121 tgacggagcc ggatttttcc cactgccaga tccaaatcat cgtagattac gactaattcc
    15181 tctggataaa tcccaaaata agtcattagt gggccgactg cacgaccaga ttcattcatg
    15241 aatgtttgtg gtttgactaa aaggatcttt tctccattat gaaaaaattc ggcaacttct
    15301 gcttcgaacg gatttttttt gaaagtagct tgatgctcgc gtgcaatttt atccacgacc
    15361 ataaagccga tattgtgttt tgtatattgg tatttggtac ctgggtttcc taacccgacg
    15421 atcattttca tttttgtttt ccacctaatt ctaaaaagca gcacaaaagg ctggactgct
    15481 aattagcggt ccagtccatc gagctttttt cttttttata cttgtcaatg catagtataa
    15541 cacaaaacaa ggcgtttcgg ctaaaagaga atcgttaata aatgataaat aaattaaaaa
    15601 tttcttcaaa gtttaatgaa ataattgata tataaaaata gtgaaataac gcacatgtaa
    15661 gcttttacat ataatacaac cttgttatct gttatatttt atagccaaat agcgtgacat
    15721 ggggcttgag aaatgttagc ataagcatgg gaaaaaacaa atcaatggaa ggattgatac
    15781 taaatgactg cagctgcaga aaaaaaagat catcaaaaag ttattcttgt tggagacggc
    15841 gccgtaggtt ctagctatgc ctttgctcta gttactcaaa atatcgctca agaagtaggt
    15901 attgtggata tcaatactgc taaaactgaa ggagacgcaa ttgacttgtc tcacgctttg
    15961 gcatttactt cacctaaaaa aatctattct gctacttacg cagacgcaca tgatgctgac
    16021 ttagttgtta ttacagctgg cgcaccacaa aaaccaggtg aaactcgttt agacttagta
    16081 aacaaaaacc taaaaatcaa ccgcgatgtt gtcacacaaa tcgttgaatc aggatttaac
    16141 ggtatctttt tagttgccgc taacccagta gatattttga cttactcaac ttggaaattc
    16201 tcaggtttcc ctaaagaacg cgtaatcggt tcaggtacct ctcttgactc agctcgtttc
    16261 cgtcaagcac ttgcggaatt agttgacgtt gatgcacgta acgtccatgc ttacatctta
    16321 ggtgaacacg gagattcaga attcccagtt tggtcacatg caaatgttgc cggcttgcaa
    16381 atctatgaat gggtaaaaaa caacccagaa atcgatgaag aagctatggt taacctattc
    16441 tttggcgtac gtgacgctgc ttacacaatt atcgagaaaa aaggcgctac tttctatggt
    16501 atcgcagtag cattagcacg tatcactcgt gccatcttag atgatgaaaa tgctgttctt
    16561 cctttatcag tattcatgaa tggcgaatat ggattgaacg acatctacat tggtgcacct
    16621 gctgttatca accgtcaagg tatccaaaaa gtaatcgaaa ttcctttaag tgacagcgag
    16681 caagaccgta tggctgcttc tgctaagcaa ttaaaagaaa ttctagatga agcttttgct
    16741 aaactagacg ctgaataatc tttgaacatg acggaaaaca tctattcggt gttttccgtt
    16801 tttttatttt atcaccctta tggtttctaa attttttcat aaagtagaga taaccttcgt
    16861 cttttcctct aaattatgct atcatgagac cgttccaccc taaactttta cttaaggagt
    16921 tgtcgatatg acaagatctt cgcatcgaaa aaaatcgaaa catgttgctt tgctttcagt
    16981 tcttggtgtt ctcttagtgc tgatcagctt ttatacctat cgcagtacat actacacaaa
    17041 gcattttttg ccgaatacag aaataaatgg aataaatgtc agcaatctaa cagtcgaaaa
    17101 agcaaatgaa aaattaaaag aagcttattc tgataaaaaa ctggctatta aagaagatgg
    17161 aaaagtctgg caggaagtac caaaatccga gctcggctac aaagatgatt tcacttcgga
    17221 gctttctccg atcctaaatg aacaaaacgg atggacttgg ggaatgactt atgtctcagc
    17281 ggcagaaaaa caagaaatag atcctgtcag tcaaaaccac caaaaactag acactaccgt
    17341 tcagacattg acaactaagc tgactgattt gaacaaagac cggacaccta ctactgatgc
    17401 aacgatcgaa aaagcaggtg actctttcgt tatcaagcct gaagtcaatg gaaatacgat
    17461 cgacgtcgat gctgcggtca aacaattaaa aagtgcggta aactctggta aagatacaat
    17521 tgaattgact gaattcaaag aaaaaccaaa agtaacatct gaagacagct cactgaaaga
    17581 acaactcgca tccatgaatg ctatcgcaaa tgtaaaagct acttacagta taaacggtga
    17641 gactttccag atcccatcaa gtgatattat gagttggctg acctacaatg atggaaaagt
    17701 cgatttagat acagaacaag tacgtcaata tgtaacagat ttaggcacaa agtataacac
    17761 aagtacgaat gacacaaaat ttaaaagtac aaaaagagga gaagttacgg tcccagttgg
    17821 cacttacagt tggacgatcc aaacagacag tgagacagaa gcattgaaaa aagcgattct
    17881 ggcaggccaa gattttactc gttcacccat tgtgcaaggt ggcacaaccg ctgatcatcc
    17941 attgatcgaa gatacgtata tcgaagttga tctggagaac cagcatatgt ggtattacaa
    18001 agacggaaaa gtggcccttg aaacagatat cgtttccgga aagccaacta cacctacacc
    18061 tgctggagtc ttctatgttt ggaataaaga agaagatgca actttaaaag gaacaaatga
    18121 cgatggaaca ccttacgaga gtccggtaaa ctactggatg ccgattgact ggacaggtgt
    18181 tggtatccac gattcagact ggcaacctga atacggtgga gacttatgga aaacccgcgg
    18241 ttcacacggc tgcatcaaca ccccaccaag tgtaatgaag gaattattcg ggatggtcga
    18301 aaaaggaacg cctgtattgg tcttctaaca aaaataatat ataaaaagaa agcacccgta
    18361 cgattggtac aggtgctttc tttttattat ctaacaatgt gctacttaca tttcagcagc
    18421 agtaaagact tctgaaacat catcatcgtc ttctagctta tctactagac gttccaattg
    18481 ctctttttgt tcctcattta aatcaatcgt cgtttgtgga atcatcgtca attctgcttg
    18541 cgccaaagtg aactcttttt ccaactcatc tcgtacagca gcaaaatctt ctggtgcagt
    18601 ataaatctcg aatacttcag gagaagtttg cagatcttct gcccccgcct ctaatacatc
    18661 ctcaaacatc gtctcttcat caacagtcaa cccatcgcgt tcaatagcga tatatccttt
    18721 gcggtcaaac aagtaactga ctgaacccgt ttctcccagt gatccaccgt ttcgagtaaa
    18781 agctactcgg acatttgtcg ctgtccgatt acggttatct gtcaatgcat ggactaagat
    18841 tcctactcca cccggaccat atccttcata cgtgatttca tcgtaattcg tttcatctac
    18901 ggcactcgta gcctttttaa tcgcccgttc cacattgtcg ttaggcatat ttgcagcttt
    18961 tgctttgtcc acaacaagcc gcaaagctgg attcatagat ggatcaggac cgcctgcttt
    19021 agctgccata taaatttcac gagaaagttt ctgaaagact ttccctcgtt ttgcatcttg
    19081 ggcatttttt cttccttgga tattatgcca tttactatgt cctgacatga tttttcctcc
    19141 cttgttcttt tgagaataca cttctattct agtactattt cacaaaaaaa caaagccaca
    19201 gccaatctat aaacgcttct tgaatgccgc aaaaagcaca cataatatca cagcagtcag
    19261 agcaccgatc gaatccaaca tgacatcttg gaacataggt gttcgtccac cggtcatcat
    19321 ctgatggaat tcgtccaaac ctgcgtaccc tgttgctgaa agccaagcaa ggatcgctgt
    19381 cagccaccaa atttttagct tggggaaaac acctaaaaac aagctgcctc ccaaaataaa
    19441 ataggtaccg aaatgtgcgc ccttccttaa aaagaactct aaaaatttgg cgtagcccag
    19501 atggtcgata ctcaccatac ttccaccata attaaaacgt atacccgcta atgtgtcttt
    19561 aaatggctga ttaggcagcc agctttctaa tcgcgaaact tgtgattgct gtccgtaagt
    19621 ttgagaagaa ctgtaaaata agattataac gactatcagt gcgattacta aataaatatt
    19681 tccgtttttc atttgttttt ttgaagaatt cataagtcac ctctgtattt tatcttagac
    19741 tttttttgaa gaaacaacaa tcattttcat agagacttct tccataaaaa agtgctatac
    19801 ttaattttgt attacaatat gaagggagtt ttttcatgag ttattcatta aattggaatc
    19861 tagattctat tttttccgga ggcagtcatt ctgatgcttt gaaccaacgg atgaagcaat
    19921 tggaagacca aatgaacgag tattatcacc gcgtaacaaa atggtcacct agttctgaca
    19981 atgcagagca gttaaacgct attcttcaat tgcaagaaac gattacgaat ggttttaccc
    20041 aatgcagcag ctatattact gcattgcttt ctgccaatgt caatgattca gatgcaaagg
    20101 tcttatcagg caagctttac gcaatgctgc ctcgtctgca atcagccgaa actgtattgt
    20161 caaagaaatt tgcagaaata tctgacaatg attggaatca gatgctttct caatcttcgt
    20221 ttgaaacaat tgcttttcgt ttaaacgaga ttcgtcgtga tggctcacaa ttattatcag
    20281 aagctgaaga aaacattata aatacgcttt ctttagatgg tctaaatgct tggagcagtc
    20341 attatgatac gattgtagcc agcatttcga ttcccttcga acaagatgga caagtagtcg
    20401 aattatctgc tggacaagcc ttcaataaga tgatgggcga tccagatcca aaagtcagag
    20461 aaacactttt cgcagcatgg gaagaagcct gggaagaaaa ggcacccctc ttcacggata
    20521 ctttgaatca tttagatggt tttagacttt ctgactatga acttcatggt gtcacagatt
    20581 ttctacaaaa accattagag tacaatcggt tgaagaaaga aacattgtct gtcatgtggg
    20641 atacaatcca gaaaaacaaa caaccccttg ttaattactt gactagaaaa gctaatcttt
    20701 tcggtaaaga aaaaatggaa tggcaagatc aagatgcacc tattatttta ggcgatttga
    20761 aagaaaaaac ctttagtttt gacgaagcag cagcatttat tatcgaaaac tttaacaaat
    20821 tcagtccaaa aatggctaaa tttgcccaat ctgcttttga aaagagctgg atcgaagcag
    20881 aagatcgtcc tgggaaacgt cctggtggtt attgtactga gttaccagaa acaaaagaat
    20941 caaggatctt tatgacctac agtaattctg ttaatgaggt tgcgacactt gctcatgaac
    21001 taggacatgc tttccatagc agcacgatgt gggatttacc ttctttaaat cgcgaatatg
    21061 ccatgaatgt cgcagaaaca gcaagtacat tcgcagaact gatcgtagcc gatgctacat
    21121 taaaagtcgc aaaaacaaaa gaagaaaaaa tcaatttgct agatacaaaa ctgcaaaatg
    21181 ctttagctat gttcatgaat atccattccc gattcatttt tgaaaatcga ttctatgaag
    21241 cacggcaaga aggattagtc tcagaagata agatcacaga gatgatggtt gaggcgcaaa
    21301 aagaaggcta tcaaggagct ttagctactt accatccata tttctgggct gcaaaattgc
    21361 atttctttat tgatgacgta ccgttttata acttcccata cacatttggc tacctcttta
    21421 gtttgggcat ctatgcttac gccaaccaaa agggagcaaa ttttgaagaa gaatatatcc
    21481 atttactacg cgatactgcc tcaatgacga cggaagatct tgcgaagaaa catttgaatg
    21541 tcgatcttac ccaaccagat ttttggcaag caggtatcga catggtgact gaagatatcc
    21601 aaacgttcat ggaattgaca gaagaattta tctgatttct attttatcac aataaaaaa
//
LOCUS       DACETN010000044        20801 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DACETN010000044 DACETN010000000
VERSION     DACETN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.046"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..300
                     /locus_tag="IXF98_001527"
     CDS             <1..300
                     /locus_tag="IXF98_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1555846.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IXF98_001528"
     CDS             complement(724..894)
                     /locus_tag="IXF98_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1555847.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IXF98_001529"
     CDS             complement(1088..1267)
                     /locus_tag="IXF98_001529"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555848.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1654..2766
                     /locus_tag="IXF98_001530"
     CDS             1654..2766
                     /locus_tag="IXF98_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1555849.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IXF98_001531"
     CDS             complement(2844..3137)
                     /locus_tag="IXF98_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1555850.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IXF98_001532"
     CDS             3305..3619
                     /locus_tag="IXF98_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1555851.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IXF98_001533"
     CDS             3640..5619
                     /locus_tag="IXF98_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1555852.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IXF98_001534"
     CDS             5632..5958
                     /locus_tag="IXF98_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1555853.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IXF98_001535"
     CDS             5974..6492
                     /locus_tag="IXF98_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555854.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IXF98_001536"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IXF98_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1555855.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IXF98_001537"
     CDS             complement(7928..9433)
                     /locus_tag="IXF98_001537"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1555856.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IXF98_001538"
     CDS             complement(9525..10115)
                     /locus_tag="IXF98_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1555857.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IXF98_001539"
     CDS             10294..11940
                     /locus_tag="IXF98_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1555858.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IXF98_001540"
     CDS             11989..12708
                     /locus_tag="IXF98_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1555859.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IXF98_001541"
     CDS             13187..14134
                     /locus_tag="IXF98_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1555860.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IXF98_001542"
     CDS             14146..15066
                     /locus_tag="IXF98_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555861.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IXF98_001543"
     CDS             15107..16564
                     /locus_tag="IXF98_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1555862.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IXF98_001544"
     CDS             16909..17523
                     /locus_tag="IXF98_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1555863.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IXF98_001545"
     CDS             17516..19240
                     /locus_tag="IXF98_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1555864.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IXF98_001546"
     CDS             19237..20688
                     /locus_tag="IXF98_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555865.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3511 c         4166 g         6415 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atattcatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACETN010000045        20222 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DACETN010000045 DACETN010000000
VERSION     DACETN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.047"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(50..1234)
                     /locus_tag="IXF98_001547"
     CDS             complement(50..1234)
                     /locus_tag="IXF98_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1555866.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1259..2266)
                     /locus_tag="IXF98_001548"
     CDS             complement(1259..2266)
                     /locus_tag="IXF98_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1555867.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2438..2797)
                     /locus_tag="IXF98_001549"
     CDS             complement(2438..2797)
                     /locus_tag="IXF98_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555868.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2769..3140)
                     /locus_tag="IXF98_001550"
     CDS             complement(2769..3140)
                     /locus_tag="IXF98_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1555869.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3157..3459)
                     /locus_tag="IXF98_001551"
     CDS             complement(3157..3459)
                     /locus_tag="IXF98_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555870.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3476..3946)
                     /locus_tag="IXF98_001552"
     CDS             complement(3476..3946)
                     /locus_tag="IXF98_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1555871.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3921..4199)
                     /locus_tag="IXF98_001553"
     CDS             complement(3921..4199)
                     /locus_tag="IXF98_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1555872.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4196..5245)
                     /locus_tag="IXF98_001554"
     CDS             complement(4196..5245)
                     /locus_tag="IXF98_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1555873.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IXF98_001555"
     CDS             complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IXF98_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1555874.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6263..8017
                     /gene="ade"
                     /locus_tag="IXF98_001556"
     CDS             6263..8017
                     /gene="ade"
                     /locus_tag="IXF98_001556"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1555875.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IXF98_001557"
     CDS             complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IXF98_001557"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1555876.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8847..9626)
                     /locus_tag="IXF98_001558"
     CDS             complement(8847..9626)
                     /locus_tag="IXF98_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1555877.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9833..11044)
                     /locus_tag="IXF98_001559"
     CDS             complement(9833..11044)
                     /locus_tag="IXF98_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1555878.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11037..11774)
                     /locus_tag="IXF98_001560"
     CDS             complement(11037..11774)
                     /locus_tag="IXF98_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555879.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11912..12346
                     /locus_tag="IXF98_001561"
     CDS             11912..12346
                     /locus_tag="IXF98_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1555880.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12339..12683
                     /locus_tag="IXF98_001562"
     CDS             12339..12683
                     /locus_tag="IXF98_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1555881.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12863..13873
                     /locus_tag="IXF98_001563"
     CDS             12863..13873
                     /locus_tag="IXF98_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1555882.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13942..14886)
                     /locus_tag="IXF98_001564"
     CDS             complement(13942..14886)
                     /locus_tag="IXF98_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1555883.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14870..17590)
                     /locus_tag="IXF98_001565"
     CDS             complement(14870..17590)
                     /locus_tag="IXF98_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1555884.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17587..18807)
                     /locus_tag="IXF98_001566"
     CDS             complement(17587..18807)
                     /locus_tag="IXF98_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1555885.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19088..19933)
                     /locus_tag="IXF98_001567"
     CDS             complement(19088..19933)
                     /locus_tag="IXF98_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1555886.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         6174 a         4068 c         3324 g         6656 t
ORIGIN      
        1 aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca
       61 gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta
      121 cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat
      181 cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat
      241 tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac
      301 cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt
      361 tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac
      421 cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta
      481 aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta
      541 atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata
      601 caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt
      661 ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt
      721 tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt ggcattgttg
      781 agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg
      841 ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa
      901 tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa
      961 caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca
     1021 actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga
     1081 atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga
     1141 cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg
     1201 aactcccagc attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt
     1261 acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt
     1321 cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt
     1381 ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca
     1441 attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt
     1501 gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat
     1561 atttcattga ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg
     1621 ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg
     1681 caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat
     1741 ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca
     1801 caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca
     1861 agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata
     1921 gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg
     1981 cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt
     2041 cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg
     2101 tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg
     2161 cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag
     2221 cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt
     2281 ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt
     2341 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
     2401 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
     2461 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
     2521 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
     2581 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
     2641 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
     2701 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
     2761 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
     2821 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
     2881 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
     2941 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
     3001 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
     3061 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
     3121 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
     3181 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
     3241 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
     3301 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
     3361 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
     3421 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
     3481 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
     3541 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
     3601 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
     3661 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
     3721 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
     3781 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
     3841 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
     3901 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
     3961 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
     4021 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
     4081 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
     4141 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
     4201 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
     4261 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
     4321 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
     4381 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
     4441 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
     4501 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
     4561 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
     4621 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
     4681 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
     4741 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
     4801 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
     4861 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
     4921 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
     4981 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
     5041 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
     5101 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
     5161 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
     5221 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
     5281 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
     5341 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
     5401 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
     5461 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
     5521 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
     5581 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
     5641 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
     5701 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
     5761 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
     5821 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
     5881 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
     5941 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
     6001 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
     6061 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
     6121 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
     6181 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
     6241 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
     6301 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
     6361 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
     6421 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
     6481 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
     6541 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
     6601 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
     6661 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
     6721 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
     6781 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
     6841 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
     6901 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
     6961 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
     7021 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
     7081 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
     7141 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
     7201 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
     7261 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
     7321 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
     7381 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
     7441 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
     7501 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
     7561 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
     7621 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
     7681 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
     7741 tgtcaacaaa cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
     7801 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
     7861 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
     7921 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
     7981 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
     8041 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
     8101 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
     8161 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
     8221 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
     8281 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
     8341 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
     8401 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
     8461 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
     8521 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
     8581 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
     8641 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
     8701 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
     8761 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
     8821 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
     8881 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
     8941 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
     9001 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
     9061 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
     9121 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
     9181 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
     9241 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
     9301 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
     9361 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
     9421 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
     9481 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
     9541 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
     9601 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
     9661 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
     9721 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
     9781 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
     9841 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
     9901 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
     9961 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
    10021 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
    10081 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
    10141 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
    10201 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
    10261 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
    10321 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
    10381 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
    10441 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
    10501 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
    10561 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
    10621 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
    10681 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
    10741 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
    10801 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
    10861 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
    10921 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
    10981 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
    11041 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
    11101 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
    11161 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
    11221 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
    11281 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
    11341 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
    11401 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
    11461 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
    11521 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
    11581 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
    11641 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
    11701 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
    11761 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
    11821 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
    11881 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
    11941 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
    12001 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
    12061 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
    12121 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
    12181 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
    12241 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
    12301 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    12361 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    12421 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    12481 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    12541 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    12601 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    12661 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    12721 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    12781 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    12841 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    12901 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    12961 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    13021 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    13081 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    13141 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    13201 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    13261 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    13321 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    13381 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    13441 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    13501 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    13561 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    13621 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    13681 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    13741 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    13801 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    13861 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
    13921 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
    13981 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
    14041 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
    14101 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
    14161 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
    14221 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
    14281 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
    14341 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
    14401 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
    14461 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
    14521 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
    14581 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
    14641 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
    14701 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
    14761 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
    14821 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
    14881 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
    14941 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
    15001 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
    15061 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
    15121 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
    15181 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
    15241 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
    15301 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
    15361 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
    15421 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
    15481 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
    15541 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
    15601 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
    15661 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
    15721 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
    15781 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
    15841 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
    15901 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
    15961 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
    16021 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
    16081 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
    16141 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
    16201 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
    16261 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgatgaga
    16321 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
    16381 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
    16441 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
    16501 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
    16561 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
    16621 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
    16681 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
    16741 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
    16801 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
    16861 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
    16921 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
    16981 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
    17041 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
    17101 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
    17161 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
    17221 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
    17281 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
    17341 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
    17401 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
    17461 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
    17521 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
    17581 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
    17641 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
    17701 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
    17761 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
    17821 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
    17881 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
    17941 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
    18001 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
    18061 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
    18121 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
    18181 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
    18241 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
    18301 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
    18361 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
    18421 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
    18481 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
    18541 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
    18601 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
    18661 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
    18721 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
    18781 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
    18841 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
    18901 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
    18961 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
    19021 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
    19081 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
    19141 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
    19201 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
    19261 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
    19321 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
    19381 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa ataaaaaatt
    19441 tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa gggattgaac
    19501 aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat tcactcccta
    19561 cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa cttccgagat
    19621 ttgtatatat tttggttgga aatttcgata ggacagaatc aatttttttt acatcatttt
    19681 tatcgaagga agactcaaat atcttttttt ctccatcaaa tccaacagaa ccatttaacc
    19741 ctactgctgg tatccttaaa ccagattttt tgagaatatt attaatgtct tctacttctc
    19801 ttccagaaca aatgaatgga aaacagtctt ccggtaaatg tttcattgtt cttgcatttt
    19861 tttgactaat gctctggtca gaattcaaaa gagtaccgtc taaatctata ccaattagtt
    19921 tcatttcatc catgtctcct atacgttgat tataaattca ctgcttattg taagtctaaa
    19981 tttctcacaa aacatatact aattatacct aatttaagta agtacaaaca tcattttaag
    20041 aataggaaag gacatttata tactattctc tgtatttttg atttataaat aaatatgaat
    20101 tgaatccaaa tcagctatat tactatttta taataaccaa ctataaaaaa cgaagttagg
    20161 tgaaaaacta acttcgatat tcataaaaga ctctattagt tcaacttagc aaaagattta
    20221 aa
//
LOCUS       DACETN010000046        19448 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DACETN010000046 DACETN010000000
VERSION     DACETN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.048"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            129..3656
                     /locus_tag="IXF98_001568"
     CDS             129..3656
                     /locus_tag="IXF98_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1555887.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3653..7375
                     /gene="addA"
                     /locus_tag="IXF98_001569"
     CDS             3653..7375
                     /gene="addA"
                     /locus_tag="IXF98_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1555888.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7444..7785
                     /locus_tag="IXF98_001570"
     CDS             7444..7785
                     /locus_tag="IXF98_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1555889.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8094..9137
                     /gene="pheS"
                     /locus_tag="IXF98_001571"
     CDS             8094..9137
                     /gene="pheS"
                     /locus_tag="IXF98_001571"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1555890.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9142..11562
                     /locus_tag="IXF98_001572"
     CDS             9142..11562
                     /locus_tag="IXF98_001572"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1555891.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IXF98_001573"
     CDS             complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IXF98_001573"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1555892.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12996..13733
                     /locus_tag="IXF98_001574"
     CDS             12996..13733
                     /locus_tag="IXF98_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555893.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13747..14562
                     /locus_tag="IXF98_001575"
     CDS             13747..14562
                     /locus_tag="IXF98_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1555894.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14575..15222
                     /locus_tag="IXF98_001576"
     CDS             14575..15222
                     /locus_tag="IXF98_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1555895.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15238..15894
                     /locus_tag="IXF98_001577"
     CDS             15238..15894
                     /locus_tag="IXF98_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1555896.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16056..16877
                     /gene="racE"
                     /locus_tag="IXF98_001578"
     CDS             16056..16877
                     /gene="racE"
                     /locus_tag="IXF98_001578"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1555897.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16880..18235
                     /gene="rph"
                     /locus_tag="IXF98_001579"
     CDS             16880..18235
                     /gene="rph"
                     /locus_tag="IXF98_001579"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1555898.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18236..18754
                     /locus_tag="IXF98_001580"
     CDS             18236..18754
                     /locus_tag="IXF98_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1555899.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18861..19361
                     /locus_tag="IXF98_001581"
     CDS             18861..19361
                     /locus_tag="IXF98_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1555900.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5393 t
ORIGIN      
        1 tgcgtatact taaaaaagta gaaatatcag aaccttaaaa ggatataatt tgaagaatat
       61 agaacaaatg ttcagtattt tttgatataa taaaggagca ccgtatcatg tgttgaagga
      121 ggaaaagcat gtctttacaa tttattcttg gaaacgggaa aaaagatcac cgtcaagtct
      181 tgatcgacga agcatccgct tggctcgacc aggatgcaag taaccaagta tttttccttg
      241 ttcccaacta cagcaaattc gaacaggaac aagaaatttt agcggagatg cgaagaagaa
      301 gcggaaaaaa aactttcagc acaacaaacg ttcaagtatt tagtttttat cgtctggctt
      361 ggtatttgct gcaacagaca actcttctta caggaaatga attatcagaa tcaggatctg
      421 caatgatctt gcgacagatt ttagaaaaaa atgaggaaga attaacaatc tttcgcggag
      481 aaatcaataa aagagggttt attcgtcaat tacaagaact ctacagtgaa ttgcgtacag
      541 gaaatattca accagaagac ctgatgcttt tatttggtga ttcacctaat gcagaagacc
      601 agcaacttaa aatgaaagac ttgaagctga ttttttcagc ttatgacagc gaattgactc
      661 aacgtgcact aacaaatgaa gatgcactat ctatactagc aaattatatg cagacacaag
      721 atttcaacaa cgtcaaattt attgtcagtg gtttttctcg aatcaatggt caagagtatc
      781 aattactgca ggcaatgatg gaaaaggggc aattggttgt cgatttattg ctggatcgtc
      841 cttatacagc ggatcttcca gaagtactta cgctctttca tgagactgga aagctttatt
      901 tccgtctttt tcaaagtgca agagaacgga aagtccctat attagtggac cgatttgcac
      961 cagagcagac tcgtccgaca gagcttcaac tgctgcaaac aatgtgggaa gagacatcta
     1021 gccaaaagaa agcggcagta cacaaaatag ggggacaaga gcatctccat ttgtgggaag
     1081 ctgaaaatcc aactgaagaa gtccgacaat tagctgttga aatcagacga ctagtcagtg
     1141 aagaaaacta ccgttatcgt gatatccaag tgctgacaaa aaatatgtct ttatatggaa
     1201 atatccttcg gccgatcttt caagaaatgg caattccttt ttatatcgat gaagaacaaa
     1261 tgatggaaaa tcatccgctg atcgaatgga tcaacagtct gttcgctctt gaccgctatt
     1321 cttatcggct aagtgatatt atgcgttttt tcaaaactga attatttctg tcgacggaag
     1381 cagaaaccga acttgatgaa tggaaacaag agcgagatga attcaggaaa aaactggaca
     1441 tcactgaaaa tgtagcactt gcttacaatt atcaaggaac ttattggact cgtgaaaaag
     1501 actggcagtt tgtggcgtat gattttgaag cagaacagat tgaagacgta cagtatttgg
     1561 aagaacagtc aaatacgatt agacgattat ttcagaaagc tgtaccagta tttttgaaaa
     1621 aaataaagaa aagcaaaaca ggacaagagg ccgcaaatgt tttttatcaa tttttgatcc
     1681 aaagtggcgt acagcaacaa ttattgtttt ggcgtaatca ggaagtagac agaggaaact
     1741 tagaagcggc aagaaaccat gagcagacat ggggagcgct gattgatctg ttggatgaat
     1801 atgtagtaat ttatggtgaa tcggcttttg actggtcact gttccaagaa atcattacaa
     1861 gtggtctaga aaatctaagt tatggtaaaa tccctaccgc aatcgaccaa gtgcgaatca
     1921 atcgtttgga attagttcga ccaaatcaag ctgccgtcac ttttgcatta gggttgaatg
     1981 atcaagtgtt tccagaccga aaagagacaa aaggattgtt gtccagtgaa gaacgagaag
     2041 tattaaatca ggccttgcca gatggacagt ttttattcga cccatcaaag gaaagtatct
     2101 cttttgagcc tttccaagcc tatcttgttt ttctttccgg aacagatcga ttatatctta
     2161 gttatgcaca aagctatgac acagatggtt cgctgaagat gtctccttat ttgcgtagaa
     2221 tcacagaata tttatctgtt cccgtcgaga gaaaagagca tttgacgatc gaaagcgacc
     2281 ctaattgtta tgtagggact tacagaagcg gaatcaatac agtcaatagg atttatcgat
     2341 tagctttaga tgaaaaaaaa cctgtaccaa agcactggca aaagctcaaa gaattagtac
     2401 tgaattcgtc atggcgaaat tttgctttac agatttttga aagtcaggaa catttaaatg
     2461 ttcctgtttc gatgccagaa aaaatggcgg aagagcttta tggaaaagag atttatactt
     2521 caatttcacg tatggagaat ttttataatt gtgaatacaa atacttcatg caatttggtt
     2581 tgaaattgaa agaacgaact gtttatggtc taactccagc agcaactggt gatttttatc
     2641 atgaatcatt ggatcgtttc ttcaagctac tgttttcaaa ccaattatcc ttagtgaata
     2701 tgtccgatgc gcagcgtaga gactttactg aaaaagtgct gcaggaagta ttcggagaac
     2761 ttcggtttga gatattggat agttctgcaa gaatgaacta tatccgctac cagttaggac
     2821 agacgattca aaaagtgaca tgggctttgc aaaaacagag caaaaataca ggcatgcatc
     2881 ctcttcagac agagattcta tttggtcaga ttgctggagc aaaaggtatc acaggtctgg
     2941 aattaccgtt gagtaatggt ggaaaactcc atgttcgagg gaaaatcgat cgaatcgatg
     3001 tagcttctga acaagaagat acttggctaa gcgtcgttga ttacaaatct agcggacgtt
     3061 cattcgatgt cacagaagct tattatggca tggctatgca attattgacg tatctggacg
     3121 tagcattgat ggatgcggta cgtttgactg gaaaggcggc tgttaaacca gcaggatcat
     3181 attatttaca tgttcataat cctgtattga cggagacaga tgatgtagag aagaatacat
     3241 tgaaaaaatt tagttatgat ggcgtttttg tcgatgatcc cgctctttta gaagtactag
     3301 atcattcact ggaagcaaaa gaacaatcac tgatctatcc agtgaaaaaa aatgcgaaag
     3361 atgaatacca aaaaggtagt ttcagtaaag acaaattttt cacggaagaa gaactgcaac
     3421 tattcatggc gcataatcgg gaaaatatgc ggttagctgg agatgcgatt acttccggag
     3481 aaatcaaact aaatccagct tataaagata aacagcggat tgcctgcgaa ttttgtccat
     3541 tcagaagcat ctgtacgttt gatgtgatgc tgaaagaaaa taattaccac cgtctggaga
     3601 aaatagataa ggaagaagca ctgcgtagaa tattgaaaag gagtgaagaa gagtgaatca
     3661 aattccttta aaaccgcaaa atgaacgatt cacagatgat cagtggcaag cgatttttga
     3721 tcaaggagat aatctattag tctctgcgtc tgccggatca ggtaaaacta cagtgcttgt
     3781 tcgaagagtg attgaaaaac taaaaatggg ttttgatatc gatgaattat tgattgtgac
     3841 atttactgaa gctgccgcaa gagaaatgaa agaaagaatt caagaggcat tacaagaatc
     3901 cgtcaatagc gaaagtgacc ctgtacgtag acagcatttt actaagcagt tagttttatt
     3961 gcccacagcg aatatctcaa ccttacatgc tttttgcctg acagttattc gacgttatta
     4021 ttacttaatt gatattgatc cagtctttcg catgttaaca gatgaaacag aaacgatctt
     4081 gatgaaggag gatgtgtggg acgagctgag agaagcactt tatgcagaaa atgatgaacg
     4141 atttttccaa ttaacaatga atttttctaa tgatcgttca gatgacggct tgacgaatct
     4201 ggtgttttct ctttatgagt ttgcccgtgc caaccctgac ccccaaaaat ggctagaaca
     4261 gttaagcgat aattatcgtt tgcctgaggg gctagcgaaa tctcgattat atcaaaaaca
     4321 aatccgtcct ttagttttgg cagatatata ccaatgtgta cagttatatg aacagatgac
     4381 tcagttagca caaggtgaag gattagaaaa aatgcatgag caagtagctg gagaacaaca
     4441 gcagatcaag aatatctatg aagcgttctc gcaagatcgg ttagaagaag cttatgctgg
     4501 tttggaacaa ctaacttttt caacttttaa gagcagtcgg aaagcagaat tgaaagaaat
     4561 ttccaatgaa gtcaagggca tgagggataa agcaaaaaaa ctgattcagc agataagtaa
     4621 aagttatttt cctgtttccc caagccaaat ggaagaatta acggacaaag cactaccgtt
     4681 agtcgaagaa atgacaaaag tcacgcaaag cttcatggac ggtttcagta tgcgtaaacg
     4741 ggaaaaaggt gtgctggatt tcaatgatct ggaacattta gcgttgcaga ttctcacaga
     4801 aaaaacgaaa gatgtatggc tgccaagtga agcttcaaaa cattatcgaa aaaaattcaa
     4861 agaagtaatg gtagatgaat atcaagacgt gaaccaatta caagaagcca ttttatactg
     4921 gctaagagaa cccgatgata caaaaggaaa tatgttcatg gttggggatg tcaaacaatc
     4981 catctattct ttccgtttag cagatcctag tttatttatc gggaaatacg agaatttctc
     5041 aaagaaagaa ggcggacgtc gaatcgtttt ggctgagaac ttccgctcta gaaaagaagt
     5101 cctttctttt acgaatctta tttttgaaca actaatggat ccagcagtgg gtcaaatcaa
     5161 ttatgacgaa gcagccaaac tgatccaagg attttcagac tttccagaga atgaacagtt
     5221 tgagccggaa atcatgatct atgaaaaaga acaggaagaa tcagagattg agattccgac
     5281 ggatgatata ctggaagaca aaacagaagg cgaacttttt atgacaggac taaaaattcg
     5341 tcaattaatt gattcctcct ttatgattta tgataaaaaa tcaaaaaaaa gtcgtccgat
     5401 agagtacaaa gacattgttc tcttgacacc tacaaagaag aacaacttaa cgattttaga
     5461 gattttcaaa acattggata ttccattaga gatgaatgat gctcaaaact attttcaagc
     5521 gacagaaatt cgcacgatga tctcgctgct acaattaatc gataatccct atcaagatat
     5581 tccgcttgcg gctgtattgc gctcaccgat cgtgggtctg attgaaccag aattagccag
     5641 cattcgatta gctaaccgtg cacatactta ctatgatgct gtgcttgctt atcaagcatc
     5701 aaacgaagat gaattagcag ctaagctgga acattttggc aagcaactag agcattggcg
     5761 agaacttgct cgccgttctt cgatcactga cttgttatgg gatatctatt atgagacggg
     5821 atatctagag tatgtggtcg gtctgccagc aggggcacag agacaagcca atctttatgc
     5881 attggttgat cgtgccaaag cttacgagca aagttctttc agaggattat accagttcgt
     5941 gcgtttcatt gaaaaaatgc aagaaaaaga taaagactta gcagaaccag tcatttcaat
     6001 agaagataat gctgttcgcg ttatgacgat ccatgcaagt aaaggcttgg aattcccagt
     6061 tgtatttttg ttagatatga caaaggagtt caacttacaa gacttgcgca atcggtatgc
     6121 ttttgaagaa aaacttggag caggaattcg ctatatggat cctgaaacac gagtgttata
     6181 tgatacgtta ccgtttcaag ccatcaaatt ggcaaagcaa aacaaattgt tgtcggaaga
     6241 aatgcgtaaa ttgtatgtag gattgactcg ggcagaacaa aagcttttca ttgtcggttc
     6301 ctataaaaat aaagagcaaa tgatccaaac gtggtctgaa gcagctgatc atgaggagtt
     6361 agtttttgat cctgcgctac gtttgaaggg cagaagcagc ttgatgaatt ggattggtta
     6421 cggattgatc cgtcatcctg aaatgcagaa atacttagag gaagaaattt cgacttctct
     6481 tcttcaacat tcaaatgcac agttttctat ttcatggatg aatcagcaat cgatcattga
     6541 acaaagacag ttgcttgctg aaaaagaact ggttaactta gatcagcaga tgaaagaaga
     6601 tgaaacttta ttagcagatt cgttacaaaa acgtctggca tatgaatatc cttatcaagc
     6661 ttcttcacaa accacaagtt atcaatctgt atctgaaatc aaacgattat tcgaagatcc
     6721 agatgataca caagaatcac gtttgactct ggaaagcagt cagaacaaag cagcatcccg
     6781 tcaatttcgt tatacgcaag aacaactagc agaaccaaaa tttttgcaaa aagatcggca
     6841 agtgtcagca gctgctgttg gaactgctac gcatgctttg ttacaattac tgccattaga
     6901 gatgccaact acagagagta tccatcaaaa attacaggaa ttggtaagga aacggttagt
     6961 agacgaaaaa gtcgcgaaaa aagtagatgt ttcttctatt atatggttct ttcaaacaga
     7021 acttggtcaa cagctgattg ccaacaaaga aaacgtaaaa cgagaacaac ctttttccat
     7081 gctgcttcct gctgacgaag tatttcaaga ctatccaaat caagaagatg aactattaat
     7141 ccacggaatc gtcgatggat acctagaaga aaaagatcat ctcaatatct atgattttaa
     7201 aacagatttt attttacatc cggatgatcc agcagaaata gatgcaatcg tccaaaaata
     7261 tcaaggacag ctaagacttt accaacaagc aatgtcagaa gctttgaata agccagtaga
     7321 aaatgttttc ctaattttgc ttcgagtgaa acaaattata aacattaaca aataattata
     7381 attgagcaaa cttgacttat gttttgtaag gattatgcta tactctggga agaggtgagt
     7441 atgatggagc taacacagac agaattcgtt ttatgcccta aatttgaaaa aacattctct
     7501 attttaggaa aaaaatggaa cggattgatt atcgatgtac tactcgaaaa cggtccgcaa
     7561 cgctttgttg agcttgcggc aaaaatcccg atggtcagtg atcgagtatt agtcgaacgc
     7621 ttgaaggaat tagaaaaaga aggaatagtc actcgaaact ttgcctgtag cgaaaagagc
     7681 cgttcagaat atctcttgac tgaaaaagga gcagacttgc aaaaagcgat gcgtcaaata
     7741 caagtttgga gcgaaaaatg gatcacagag gaagaatgcg gttgattttg taaaaaaaat
     7801 cttgtaaact atagtccata cgaataagta aagaacgatg acgaaaaaga gtagtttggc
     7861 taaaccagtt cagggagatc tgtcgcagac tgaaagcaga tcctgtgttt gaaaaatgaa
     7921 gttcactttc cgagttcgtc tgataaaatc agaccggcaa ttgtcgttaa cgaagtaaga
     7981 gtgcggtgta agtcatttat ccgcaaatta ggatggtaac gcgaagacct cgttccttaa
     8041 caggaatgag gtctttttgt tttgtatttg gaatttaaag gaggaacaag cacatgtctc
     8101 tacaagcaca attagagagc ttgaaagaag caacgctcgt caaaatcaca gcggcacaag
     8161 atttaaatga attaaatcaa atacgtgttg aaacgttagg aaaaaaagga ccgatcacag
     8221 aagtattacg tgggatgaaa gatctaagtc cagaagagcg accaaaagtc ggaagtttcg
     8281 caaatgagat ccgagatctt ttgacagaag agatcgagcg aaaaaaacaa gtattagaag
     8341 aagaagcgtt aaatgcagct ttggcaaaag aaacaataga cgtgacacta cctggtagaa
     8401 aagtaaaaca tggtacacgt catatcgtga cacaaatcat ggaagaaatt gaagatattt
     8461 ttgtgggtat gggctatcag atcgtagaag gatatgaagt agaatctgat cattacaatt
     8521 tcgaacggat gaatctgcca aaagatcatc cagcaaggga catgcaagat actttctata
     8581 tttcagacga gatattgatt cggacacata cttcaccagt ccaagcacgg acaatggaaa
     8641 aacatgattt ctctaagggt gctctacgaa tgatctcgcc agggaaagtc ttccgcagag
     8701 atacagatga tgcgacccac agtcatcagt tccatcaaat tgaagggcta gttgttgata
     8761 aaaacatcac aatgggcgac cttaaaggaa cattagaagt cgtaatgaaa aaaatgtttg
     8821 gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc atttacggaa ccatcagtag
     8881 aagtagatgt cagctgtttc aaatgtggtg gtgccggttg taacgtatgt aaatacactg
     8941 gatggatcga aattttagga gctggcatgg tacacccgaa tgtgttgaag atgtcaggaa
     9001 tcgatccaga agaatattca ggctttgctt tcggccttgg accagaccgc gtagctatgt
     9061 tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa tgacttacgt ttcttaagtc
     9121 agttcaaggg ggagtaagaa aatgttagtt tcatataaat ggctcaatcg ttatgtagat
     9181 ttatcgaatg taacaccaaa agaattagcg gataagatgt ctgtgacagg aatcgaagta
     9241 gagggagtaa cggttccaga agaaggcttg aaaaaaatcg ttgtcggtga agtcaaggaa
     9301 tgcgtaccac atccagattc tgatcacctt tctatttgtc aagtcgatat tggcgaagac
     9361 gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg ttggaatcaa agtgattgtt
     9421 gcacttccta actcacgtat cgctggtaat gtaaaaatca aaaaaggaaa aatgcgtggc
     9481 caagtatcaa atggcatgat ttgctcatta caagaaattg gctattcaga tagtgttata
     9541 ccgaaagaat acgctgaagg tatctattat atgccacaag aagctgtaaa tggcgatcca
     9601 gtttttcctt atttggatat ggatgattcg attattgaat tatctatcac accgaatcgt
     9661 gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg gggcaattta tcgtcagact
     9721 cctacattcg atgacgaaaa gctggtagaa tcctcacgtc ctgctagcga taaaattcaa
     9781 gtggaagttg aagataaaga agcagttcct gcttatcaaa tcagaatcat tgaaggagta
     9841 agaatccagc caagtcctca atggctgcaa aatcttttga tgaacgaagg cattcgtcca
     9901 atcaacaatg tagtagatgt gacgaactat attttgcttt tattcggaca accactacat
     9961 gcatttgact acgacaaatt gaaatcaaaa gagatctttg tccgccgtgc aaaagaaaat
    10021 gaaacattag taacactaga tggagaatct cgggaattga caactgacaa tctcgtcatt
    10081 actaacggca aagaaccagt tgctttagct ggtgtcatgg gagggttaga ctcagagatc
    10141 acgaatgaaa caacaacagt tgcactagaa tctgctttat ttgatccaat ttcgattcgt
    10201 cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg cacgttttga aaaaggaatc
    10261 aaccatgcga ctgttggaga agccagtgag gttgctgcag caatgatcgt ttctcttgcg
    10321 ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga tcaaagcaga agacgtatca
    10381 gtgaagattt cacttgaacg catcaatcaa tatttaggaa catctcttgt cgttaaagaa
    10441 gtaaatgaga tttttgaagc acttggattt agctatgaag aaaaggagta tgtttattcc
    10501 gtagtagtac caccaagacg ctgggatatc caaatcgaag cagatattat cgaagaagtt
    10561 gctcgtattt atggatacga ccagttgcca tctacacttc caagtggaga aacggttgct
    10621 ggaagcttgt caaaagaaca agaaactact cgtaaaattc gtacaatctt tgaaggaagc
    10681 ggattgagcg aagcaatcag ttatgcattg acaacagaag aaaaatcaag acagtttgtt
    10741 ataaatgaaa gtaatattac acgtttggac tggccaatga gcgaagaacg ttctgtatta
    10801 cgaatgaatt tgatcagcgg actattagat gatatcagtt ataacactgc ccgaaaaaat
    10861 acagagattg ctttatatga aattggtcgg gtgttcttcc aagaaaatga tccacgtaca
    10921 catctgccgc aagaagtaaa acatgcagca atcgctattt ctggtatttg ggaagaaaaa
    10981 gattggcaga cgaaagctca aaatgtagac tttttcacca tcaagggtct tttagaaaac
    11041 ttatttgaac aactaggcat cactgaagat gtccattatc aaacagtgac agaaatgaag
    11101 gaaatgcatc ctggaagaac tgctgcgatt taccttggcg aaaaatttat tggttttgtc
    11161 ggtcaagtgc atccaacgat tacaaaaagt tatgatattc cagaaacata tgttgctgag
    11221 tttgatttat ctgctatagt ggaagctgct caaaaaggga tcgttttcga agcagtgaca
    11281 aaattcccag cagtcagccg ggacatcgct ttgcttgtaa aagaaactgt gacaaatcag
    11341 gaattaacag aagttatccg ttctgctgcc ggaagatttt taacagatat ccaattattt
    11401 gatgtctatc aaggagaaaa cattgaaaaa ggacataaat ccatggcgta tagcctgaca
    11461 ttcgtcaacc cagaagcaac attgacagat gaagaaatca ataaagcaat ggaaaaagtc
    11521 gaaaaagcgt tggtagaaaa cctagaggca agtattcgct aagaaggaaa atatagtaag
    11581 taacgatcaa gtagcttttg aagaccatac atttgaaaaa aagatgtgac acaacttcgt
    11641 cacatctctt tttttgattt taaaaaagga atagcacaga gacgagataa aaaaacaggc
    11701 ctttacagcc tgctttttta gagtaaatga attaatggaa gtaattgatt cccattgctg
    11761 attttacttc agaaagtgtt tgtgcagcta ctttagcagc ttcttcacta cctttacgta
    11821 agatttccat aacagctgaa ggatcttttt ccaattcttc tcggcgcgca cggatcggtg
    11881 caaattcagc ttccaataca tcaatgaggt atcgtttgat ctttacatca ccaagtccgc
    11941 cgcggcggta atgagctttc atttcttcaa ttgcttcttt atctgttccg aatacatcca
    12001 agtaggtgaa gaccatattc ccttctactt gtccaggatc ttgtacatgg atgtggtttg
    12061 gatcagtata catactcatg acttttttct gtaagacatc ggcaggatcg gagatataaa
    12121 taccgtttcc taacgattta ctcattttgc catttccatc gattccaggt aatctaccca
    12181 ttccttttgg tgggaatacg ccttttggtt ccaccaagac ttcaccatat gtatgattga
    12241 agctttgtac gatttcctgt gtctgctcta gcattggttt ttgatcctca ccaacaggta
    12301 caagattcgc tttgaatgca gtgatatctg ctgcttgaga aacaggatag ataaagaatc
    12361 ctgtaggcac gctttcaccg aatttttttt gttcgatttc tgttttgaca gtcggattgc
    12421 gccgaacgcg tcctacgctg actaagttca aataatacat ggttaattca gctaattccg
    12481 gaatttgtga ttggataaat aaagtagatt ttgcaggatc taatcccacg gctaaatagt
    12541 ctaaagcaac ttgcaagaca tttgatgaaa ccttctcagg atttttagca ttatctgtca
    12601 gtgcttgcat gtcagcaatc atgacaaaca gttggttgtc ttcatctgcc tgcatagcca
    12661 ctcgggtttt tagagaaccg acgtaatgcc ctaaatgcag cttcccagtt ggtcgatctc
    12721 ccgttaaaat aatatttttc aaaaaagaca cctctttacg atttgtaagt ctgagcactt
    12781 aatatcttac cgtttttcct gaaaagaaac aacagttcct aaggaaaaaa caaggaaaga
    12841 ggataaaata actaatagat tagaaatgga agaaatattt atgggagaat aaacgttcta
    12901 agaaacttta caaattacta aactatcaga tataatgtat tttattatac atctttaaaa
    12961 gaaatcttac taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg
    13021 tggataaata ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag
    13081 gagaagttgt cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta
    13141 tcaatggatt agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg
    13201 ataaaaagac aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca
    13261 atttgtaccc aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga
    13321 agcaagacca ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt
    13381 tagataagaa agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa
    13441 tcgccagagg tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt
    13501 tggatccgga aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta
    13561 tgtcaatgat tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta
    13621 tcttcatggc tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa
    13681 atccaaatga agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg
    13741 aaaaagatgc gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt
    13801 gcactagccg gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa
    13861 tctaccaatg aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat
    13921 attgagagca gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag
    13981 atccttggtg acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca
    14041 ttattgaaaa acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga
    14101 aaaaaacagg ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag
    14161 aaaggaagcc aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc
    14221 aaaggttcta catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa
    14281 ttagaaaact atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact
    14341 acggataatg cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt
    14401 ggaacgttta ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg
    14461 aaagctgtta accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat
    14521 caaaaatggt ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc
    14581 gaattgatac aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg
    14641 agtgtcttag cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta
    14701 tcaaagaata aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt
    14761 ccattattaa tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta
    14821 gatggattcc aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa
    14881 acagtacgtt cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct
    14941 ggttttacct attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt
    15001 atcccgccat taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc
    15061 gtagctggtt tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc
    15121 ttcgatacgt atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac
    15181 ttaatggttt atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg
    15241 gattttagcg gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt
    15301 actgttgaag tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt
    15361 gtgattcgtt ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt
    15421 atacggaatc ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc
    15481 ggcattcaga tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct
    15541 atgttgtcgg aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca
    15601 ggattatcaa cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca
    15661 tttaaatcaa tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca
    15721 ttagcagtaa tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag
    15781 aacaccaatt atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt
    15841 tacgcgcttt ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg
    15901 attcttttag cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa
    15961 aaaagagatt tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta
    16021 cacaataggg aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc
    16081 ctatcggatt tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac
    16141 aattaccgaa tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta
    16201 gacccgcgga acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag
    16261 gaatcaagat gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca
    16321 aagctgctct ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa
    16381 aaaaaacaca aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag
    16441 cttatgaaaa agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc
    16501 ccaaatttgt ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg
    16561 tggcagaaac attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca
    16621 cccattatcc attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga
    16681 tcgattctgg agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga
    16741 gcaattcacc gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac
    16801 ttttcgagga aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg
    16861 aattgggagg aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact
    16921 ggagcttcaa acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga
    16981 tacaacagtt atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac
    17041 aggaacagga tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa
    17101 tcgtagagaa agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat
    17161 tggtcgttcc ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt
    17221 agactgcgat gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt
    17281 tgtggcattg aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc
    17341 aataaaagaa catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac
    17401 agatttggat taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga
    17461 atctggaaaa ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca
    17521 gttgaatgaa atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa
    17581 acatgctttg ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat
    17641 catcattgcg acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc
    17701 gggatatcat gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg
    17761 cagcacattt gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca
    17821 gccagtctta gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat
    17881 ctattctgca cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt
    17941 atatgaattg acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt
    18001 attcgcggca cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc
    18061 ccgtatacca tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa
    18121 gaaacaaaca gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca
    18181 agcaatgaaa aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg
    18241 gtatttagta gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg
    18301 ttatcgttct gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga
    18361 cccattatgg gatacattcc aagtggttca aggtaattgt gattatggac caggattcga
    18421 acagaaaaaa gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc
    18481 gaatgtccga tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat
    18541 ggtgtttttt ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt
    18601 gaatcctggt agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat
    18661 cgaaagttta gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga
    18721 agagttggcc ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa
    18781 aataagcttt attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa
    18841 atagaaatgg aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc
    18901 aggaaacgtt tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt
    18961 cccatggttt acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag
    19021 atgatcactt tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc
    19081 aaacaatcag catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg
    19141 ttccgacaat tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc
    19201 cttttttacc ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc
    19261 tgcttaaagc cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga
    19321 aaatcgaaga attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga
    19381 taatcaaaaa ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg
    19441 ctgggaca
//
LOCUS       DACETN010000047        19047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DACETN010000047 DACETN010000000
VERSION     DACETN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.049"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            104..298
                     /locus_tag="IXF98_001582"
     CDS             104..298
                     /locus_tag="IXF98_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555901.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            567..866
                     /locus_tag="IXF98_001583"
     CDS             567..866
                     /locus_tag="IXF98_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555902.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(963..1397)
                     /locus_tag="IXF98_001584"
     CDS             complement(963..1397)
                     /locus_tag="IXF98_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1555903.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1629..2339)
                     /locus_tag="IXF98_001585"
     CDS             complement(1629..2339)
                     /locus_tag="IXF98_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1555904.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2329..3189)
                     /locus_tag="IXF98_001586"
     CDS             complement(2329..3189)
                     /locus_tag="IXF98_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1555905.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3194..3832)
                     /locus_tag="IXF98_001587"
     CDS             complement(3194..3832)
                     /locus_tag="IXF98_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1555906.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3847..4146)
                     /locus_tag="IXF98_001588"
     CDS             complement(3847..4146)
                     /locus_tag="IXF98_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1555907.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4189..5673)
                     /locus_tag="IXF98_001589"
     CDS             complement(4189..5673)
                     /locus_tag="IXF98_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1555908.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5693..6154)
                     /locus_tag="IXF98_001590"
     CDS             complement(5693..6154)
                     /locus_tag="IXF98_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1555909.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6172..7239)
                     /gene="ulaG"
                     /locus_tag="IXF98_001591"
     CDS             complement(6172..7239)
                     /gene="ulaG"
                     /locus_tag="IXF98_001591"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1555910.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7457..8227
                     /locus_tag="IXF98_001592"
     CDS             7457..8227
                     /locus_tag="IXF98_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1555911.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8500..9327)
                     /locus_tag="IXF98_001593"
     CDS             complement(8500..9327)
                     /locus_tag="IXF98_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1555912.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9895..11130
                     /locus_tag="IXF98_001594"
     CDS             9895..11130
                     /locus_tag="IXF98_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1555913.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12129..12389)
                     /locus_tag="IXF98_001595"
     CDS             complement(12129..12389)
                     /locus_tag="IXF98_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1555914.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12416..13717)
                     /locus_tag="IXF98_001596"
     CDS             complement(12416..13717)
                     /locus_tag="IXF98_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1555915.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14009..14467)
                     /locus_tag="IXF98_001597"
     CDS             complement(14009..14467)
                     /locus_tag="IXF98_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1555916.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14480..15805)
                     /locus_tag="IXF98_001598"
     CDS             complement(14480..15805)
                     /locus_tag="IXF98_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1555917.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15822..17234)
                     /locus_tag="IXF98_001599"
     CDS             complement(15822..17234)
                     /locus_tag="IXF98_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1555918.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17251..18015)
                     /locus_tag="IXF98_001600"
     CDS             complement(17251..18015)
                     /locus_tag="IXF98_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1555919.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18119..18994)
                     /locus_tag="IXF98_001601"
     CDS             complement(18119..18994)
                     /locus_tag="IXF98_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1555920.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6049 a         3926 c         2929 g         6143 t
ORIGIN      
        1 caaattgatt ctttttattt atcataaaga tatagataca taatcatttt cacttacata
       61 tatttgaata ttaactaaaa acagcaagaa agaagaggat caaatgtatt atgtagaagt
      121 aaaaacaaaa ggcgtcaaaa acaaacaata tgtgaaaggt atcagtaacg aatatccttt
      181 acttggttca tggaaagaag ctgctccttt ttccaaacca tgtgccataa aaataaagaa
      241 cgagctagaa aaagaactga cttgtggtaa agctgttgta gatatcatcg aaaagtaaat
      301 ccaatgtata gaaaggaaga tcacacatga aaagcgacgt acaaattgct aagattatag
      361 aattagaaga agaagacgca gtgttgacaa ttgaaaaaaa ataaatgttt gtataccata
      421 tgattatttc aatatgtatc ctggaattgg agatctcgta gagctatatc aagatgaatc
      481 aaatatactt gtcattcatc atgaaatata gagacttata aaattgttta tttttataat
      541 gtatcagtat agaaaggaaa taaaacatgg attatactgt tgaatttgac agtgaactac
      601 ttaagcaatt tccacaagaa gaacatttca tgtataaaga agcaatttcc cacaaagtga
      661 cagagtattt ggagcataat ttttttcgta taaagccagt gcgtactcgt tcggcaaaag
      721 gaatatacga gatgaaagtc aaaattaata aaaaaaatta tcgcatagca tttgctttaa
      781 aaaaaaatca tgtgttcatt ttttatatta gttcgaattt gcaaaaaatt gtatttgata
      841 aagaggtgag taaaaaaata aaatagctga gcattggagg atagattgag tgatactaag
      901 tctatcctct tgtttttcta aagaactaag ctagaatttt ttcttttgta cgttattata
      961 tgttatttgt cattaccaaa aagcaccttt tttcttctag tatctatgac ataagaaata
     1021 ccacccattt cctttacttg aacaaatcct actgattcat aaaatttttt tgttttctca
     1081 gtatcatctg tcaataattc gatttggact ggtccataac tagtgatctt ctccaaagct
     1141 tcattcaaaa gcactttgcc tattccttgg cgctgatacc tcggatgaat gaggatatct
     1201 tgaataaatg cgatcgtcgc aaaatcagtg atccaacgaa cgattccgat aatcttccct
     1261 tccccattac gctttatcag agattcagat tgttcaaatg ctctagctaa ccttgcagtg
     1321 tcttttgtgt aattcgtcca accaacgctc ttatataatt cagaaatctc atcgatcata
     1381 agggaatcaa tattcatttt tttcttcctt tcttatacaa ttctccctct aataatacag
     1441 ttctttgact atttaatcta tcacatataa ctctcctgta aagattctct cgattcctta
     1501 aaattcctct ttccttttag aatgcatact ttttgataaa taaaaaggtt gtgataaatt
     1561 ttcttcacaa cctctgtttc tctctatcaa gtgttatctt ttaattttct acccttaccc
     1621 tagcatctct attgcccgta gtatgcatac tttccatgtt tgcgaaaata atgtttatct
     1681 agtaaatatt gcgggatttc acatatattg gggttgatca gctcgttttc tttggccatc
     1741 agacaaattt catctaagat caaactgttt tctactgctt tcataggtga atccccccat
     1801 gtgaacggtc catgtccata gacaagtact ccagggactt cgtttggatc taattttctt
     1861 tccttaaacg tctcgacaat cacattccct gtatgaactt cgtaagcttc tcttacttct
     1921 tctttcgtta attggcgtgt acaaggaacc ttaccataaa aagcatcagc atgagtcgtg
     1981 ccataagctg gcaaatcgcg tcctgcttgc gcccacatga cggagtgcgt ggaatgagta
     2041 tgagtaatag cagttatatc ttcaaaagtt tgatacaaaa caacatgtgt ggggagatct
     2101 gaagatggtt ttaagctatc ttcttctatt atatttcccg acaaatctgt taccaccatt
     2161 tgatctgctt gcatgcattc atatctgaca ccgcttggct taatgacgat gactcctagc
     2221 tcacggtcca cttcactcac attgccccaa gtcaatttga ctaatccgta tcgcggcaat
     2281 gccagatttg cttcaaggac acgttgcttc attgctgcaa ttttttcttc acaagacatc
     2341 atagccagcc tccttcaatt tagaacttaa gtagtctttt gcctgctgga tttctcttcg
     2401 aaagtcttgg cttttttcac tccacatttc aatcagaaat gttccatcat aatctagacg
     2461 tttcaaatgt cttaataatc caagaaaatc aacacaaccg ctaccaaaag gaacatcacg
     2521 aaactggcct gaatattctt ttgttacagg gtaagtatct tttaagtgga tagcagtgat
     2581 ataatcgatc cctttctcaa gttctcgagc cggattattt tccggccatg cggacaaatt
     2641 gcctaggtct ggatatactt gtagaaaagg tgaatgaatc tgttctttga tttctaaaaa
     2701 ttttgaaatg gagttcataa atggatcatc catgatttcc acggaaagaa tgatgccata
     2761 tttagaagct tcctttacgc tttcttttat tccttcgata aagtcttctc tggaattcat
     2821 tgatttctct tcataataaa catcgtatcc agctatttgg attactttga cgcttagatt
     2881 atgcgccaaa tggattgctt ttttcattag ttgtttcgct gcttttttct tttctggatc
     2941 agcagaacca aacggaaatc gccgatgcgc acttaaacaa atcgaatgga tacgtacacc
     3001 ggagctgaac atctcttctc tcagctcagc tatcttttct tcagaccaat ctaatcgtgc
     3061 taaccgctca tccgtctcat caatcgacat ctcgataaaa tcgaagtcca tttcttttac
     3121 aagggaaaaa cgttctttcc atgaaatatc tttgggtaac gctttttcat aaatcccaat
     3181 ttgagccatt tccttacccc cagatacgaa tgatctcatc tttgaagttt tgtgcggcct
     3241 tcatcggatc atttgctgct gtaataccac gaccagtgat aaaggtatac acatcgatcc
     3301 cttcaaataa gtgtaatgtg tgtggattta atccaccagt tacagaaaca ttaaagccta
     3361 gttcaatcag ttttttgatc ttcgataaat ctttttcacc ccaagtttca ccagataaca
     3421 gcgcatcacg gctttgatgg tagattacct gccggatacc gatgttatgc caatccatgg
     3481 cttgttcaaa agtccaattg ccgtagagtt ctacttgcaa ttcacctatt tctttttgtg
     3541 ctgcctccat agttggaatc gttgcacaac aaatgactgt catccagtct gctcctgctt
     3601 ttgcaacatt actcgccact gttcctccag cgtctgcaca tttcgtatca gcgactagtt
     3661 ttttatctgg gaacatactg cgaatacagc gaatcgcttt ttgtccttcc tgtaagcaaa
     3721 gaattgtgcc cacttcaata atatctacta catcaccaac tttcataaca tctcctaatg
     3781 catgttctaa gtcattgtga tctaacgcaa tttgcaaatt cggcctgctc atcttagttc
     3841 cttcctttac ttgttttttt ggtaccactt tgtttcagca aatttttcac tgatttcttt
     3901 gactgacatc acatttttga taccgataac tgtaactcct tttttctctg cctgagtaaa
     3961 catattcaaa aaattgactg gcgtaaaaac tacatcgtat tggccagctg aactttttcc
     4021 ctctgaaatg gcacaatgat ggattttggt aattggtacg tttagttctt tcattgcttt
     4081 ttccacactt ctcatcatca tcaagcttgt tcctgatcca tttgcacacg atactaaaat
     4141 tctcatctta atccctccat tatgagtgtt gtctaatcag attttctttt atactctctg
     4201 cttttctgca tatgtgtcat agtcttctac aatcaaaaaa taaccatcag gatttttccg
     4261 atattgtaac tgcggaatag ccaataaacc aatgacaact acggcaattc cagcataacc
     4321 taggactttc atgattactg taaacaatgg ccaagctgtt gcccagtcga acatcccgat
     4381 ataaccaccg tatgttgaca tcccaatcca tgtagcgaat aatgctgaac cgcctacttg
     4441 tatgattcct gaaagaaacg ggaacaggca agcggctttg aatccacctc ggttattggc
     4501 ataaactgct ataacggcat tatcaaagaa caacggaatg aaaccggcta tgacgatcgt
     4561 tggtgatttc aataaaatca gtaaaccaat catcaaaaac tgtcccaaag caccaaacaa
     4621 gaacccaatc gttaccgcat ttggcgaacc aaagccaaaa gtagcagcta cgtcaattcc
     4681 tggaacagct cctggtaaaa tcgtgtttga gattccttgg aaagattgag ttagttcatc
     4741 gacgaatgtc cgaacaccta attgcaagat cgccagatat accgcaaaat ttaaagaagt
     4801 cgtcataatg tagaataaga aactctgccc ttcttgcatg aattgcgctt gaatcaaata
     4861 atctttgcct agtacaatca gaatgatccc aaagaaaaat agcatgagta acgaagtagc
     4921 aaccatattc tcattgaaaa tcgacaagaa tcctggtaat ttgatatctt caattttttt
     4981 gtcttgcttg attgatctat ttttcttttc atcccgcttt ttcatccact cagctaattt
     5041 tgcaaatata aagattccga acatttgctg atgtgccact gcaaatccgg caccttctgt
     5101 caattcctgc gtgattccta ctgttaagtt agaagctact gcccaataac aacctaaaat
     5161 cagtcccata aaaatcaata cttgtatcct acctagatca ggaaagcaaa acagtaaaat
     5221 ccaaaaagcg gtagctgctt gttgaacctg aacgtttccg gtagtgaaaa cggaacgcag
     5281 tttcgtatat ttctgaaaac ggactaataa gatatttaaa acaaatgcaa tcagtaataa
     5341 aatcatcaca tctccaaagg tacggccaaa tgtctccatt aatccagcat ccacagcatt
     5401 ttgtccaaaa taaggatctg taaccatggc atccaaattg aaccgttcct ttagtccaac
     5461 caagattggc cgaaaattat tgaccaaacc accagagcca acagtcaaaa taaagtaacc
     5521 gaccgttgcc tttaagaatc cagcgatgca ttcatgaagc gggcgtttca gcaaaatata
     5581 gcctaataac acgataaaac caatcaggta tgctggctgc gtcaagatat ttgttgcaaa
     5641 atacgtccaa atgcttaaca aaaattcccc catctttttt ctcctctatc aattaattat
     5701 attctgccat cacaacttgg taatcatcga tggagttcgt agagatgaga cgctcaacca
     5761 atccatctgt cattaataat tcggataatt tttggatatt ttctagatgt tcttctggat
     5821 cttttgctgc gagtgtaaaa aacaaactag ccgttttctc ctcattctcc ccttcacttt
     5881 caaaacggat tggctgtttc aacttggtaa atgaaatagc tgttccaaaa acaccatcac
     5941 ttttttctga agaatgcggc attgctactt gaggtgcgat cacaatataa ggaccgtatt
     6001 tttctacaca atggatgatc tcctccacat acgtttcatc aataatttcc ttatcgatca
     6061 atacaagaca gctttcggcg atagcttcac gccaatccgt gatttcttta tctgggtaat
     6121 gaatcaattg atggtcaaaa aagtatttca gcaagaaact ccccttcttt cttataaaaa
     6181 cgacggaaac ggtgtatcat tttcaatcga gaagacatct tcaaatcctc gataaaaatt
     6241 aaactcaaga tcatctttgt ttgtcggata gatgaatttc ccgcctactt gccagatata
     6301 aggtttaaat ccatacttca ggcgatcctt tttgaatttc caaatttcag tgatctcttt
     6361 tggatcagcc ataaaattag accaaatatc gtagtggaca ggaatcacta cttttgcatt
     6421 caatgactcc gccatgcgca gcatatcaac agaagtaact ttgtctgtta tccctcgtgg
     6481 gttttcacca taggccccta aacagacatc gattttgtgt tcattaccat gtttggcaaa
     6541 taaattggag taatgtgaat ctcctgcatg ataaagattc cctcctgatg tttcgaataa
     6601 atagtttact gcaatttgat ccatctcctg aggcattttt ccttttaata cctcttctgg
     6661 atcttcacaa gtgaccaacg ctgtccgatc aaacgcttct aaagctacta cattgatgtc
     6721 tttgattttg actgcatcac ctggctttac tgtaatcgtt ttttcttcag gaattcccca
     6781 tttttgccac gtgcgaacca cttcttttgg cccaatgaat gttgcttcag gacaattttg
     6841 atgaactgct gcggctgtat ggatatctag atgatcggaa tggatatgtg taacgattaa
     6901 agcgtctaca ttttttatcg caaaaggatc gattacgaaa ggctgagtac gtaaattagg
     6961 ctgcatattt tgaacgccac tcatacgcat catctgatgg ccttttttca tcatgccatt
     7021 cccatgacta cgttttcccg tcccacacca tagatcacat aaaatattcg ttccttcatg
     7081 acttttcagc caaattcccg tacagcccag ccaccacata gcaaaagttc caggatccac
     7141 tacttctgtt tcgatttctt cattcaagta tgtgccccac tcaggaaatg tttctactaa
     7201 ccacttttct tttgttactt cattgacagt tttactcatt tgcttgctcc ccccatatcg
     7261 ttgttccaac ttacaagtaa atattaatgt gtatcagcat ccatgaaaac ccttttatat
     7321 gaccgtttgt tttttatatg attttctttc agtaatcaat gattgtttca gtataataaa
     7381 gacagatatg aatgtttaaa tgaacaacta gtcaaataaa tccatggatc aagatgtccc
     7441 tcaggaggca aaagtcatga aaaactcact tgtcaatatt gaaaaacgtc aaaaagaaat
     7501 tttggaactg cttcagcaga ctgaacaact atcaactttc gaattagctg aatcccttga
     7561 tgtatctatt agtacgatac gaagagattt aaatttatta gaagaaaaaa acgagatcat
     7621 acgcaattac ggttattgtt cttataatta caaaaaccag actgattttg atcaatctgg
     7681 tcctgaaagg attaaacaag cgattgccag agaagctagt aaatacatta acgattatga
     7741 tacgctgttt atcaattcta gctcgactgc gttgaaaaca ctggattttc ttcaatctaa
     7801 ccatttgaca gtcattacaa acaacgtgaa aatcaattac tctccgcaca aagataattg
     7861 tagttatatt ttaactggtg gagagatgcg ctttccaaaa gaagcactag tgggcgacat
     7921 cgcattaaat acgatttctt cagtcaatgc agacatttgt atcattggct gtagcggagt
     7981 cgatgtagta catggggtca caacaaagat attaaatgaa tcaacgatca acgaaatgat
     8041 ggtcaacaag acaatcaaat gtaagatttt agtcgctgat catcgaaaaa tcggactgac
     8101 atcaaagttc aagattgctg atatttcagt ctttgattat ttgatcactg ataaattttg
     8161 ttctgaaaaa gtattagagg agatcagaaa tacggggata aaaaccattc aagtagattc
     8221 attttaagaa ccaggacgta aatatgtaaa taggattatc aaaaaaatga ataacaaatt
     8281 tatcgataaa tgaaaaacac gctacgctca aattgataaa ggaatcccaa gatgtatcca
     8341 tccaatcgat accatcataa tctattgctt cttttataac cgttatatgt tgcctaaata
     8401 ggaatcaaac aatatcgaaa aaaggaggta tgacaaaagt cacacctcct tttttcgaat
     8461 aaacggtatt caaatcagaa cactgatttc acaaccactt tatttcaggt cgttttcaat
     8521 cacctttacc gcgtaatcat ttccgttgaa acgcgctatt ttttttgcgt ccatatatct
     8581 ttctaaataa tcatcttctc ctaattgctt atggcaagaa gcaatttcat aaagcaagta
     8641 ttccaatgca taagtcgatt tatgtttctt attatactca atacccttct cacataattg
     8701 gattgcttta tcataatcct cgattttacg gtaataggtg gcgatatcat aatatacaat
     8761 gcagaccatc agattatcaa aatcagtctc actcaatatt ttaagtgtac gctccacata
     8821 aactttcgca aaatctaatt ctccttttga caagtagatc attgccacgt ttgcagctgc
     8881 ttgtaagtga tggatatctg tttcggtcgt ttgttccaac acttgattaa aataaaataa
     8941 tgctgcatct ggttttttcg cataaagatg atcaagcagt ccttgataaa ataaaatttg
     9001 atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa ttttataaga
     9061 ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt tcctgatttt
     9121 gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt tgcaaagcgc
     9181 acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga cagttgcttg
     9241 ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc ggtattcttt
     9301 tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa attatttctc
     9361 ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa cagaataaaa
     9421 attttaattt taaaataata ttcaaattaa acaactggcg tatctgctcc ttgatttctt
     9481 tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa ttgtcatggc
     9541 gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg tataatgacg
     9601 acagcacacc tatcaattaa tataattatt ttattttttt atgttctcat gtttattttt
     9661 aatgacacaa cttctcgtat ttatctggca caacatataa atcgatcaaa aaattgttgc
     9721 gtgttttttt gaaaattgga gtttcaactt gattacaaaa tttaaattta gaagttgtga
     9781 aaacagcgag agacagttta atattcaaaa tatacatagg ctactcgaac gctgtgtttt
     9841 ttagtatact agtaagaaac gaagggatag actcattcca ataaggagga tttcatgaag
     9901 acaattcttt ttgctcctgc agtattcaat ttagctgaga caacacgaat gattgaggtt
     9961 gccaaagcta tgaaagaccg ttattactgt gtattctttg gcttttctaa aacttttgct
    10021 cctttgatca cagaaagtgg ctttgagtat catttactag cgccgatcct tacgaaaaaa
    10081 caagaagaac aaatcataaa atttgaccag ttgcgtacac ttaaaaaccc ttttaccaaa
    10141 gaaatggttg cctctcgtat cactagtgaa cagagtctga ttgctaagtt tgagcctcga
    10201 ctgattgtga caggttcgaa tgtgacaatt tttttatcgg cccgcaataa aaagattcct
    10261 ttagtatatg ttaaacccta tgctttgagt gaaccttttt ttcaatcaga gcatgcgctg
    10321 atgccccacg agttacgttt cttcggccct ttaagcaatc ccctttggca ctggacaaaa
    10381 aagcgtatag taaatacccg atggatcccc aatgccttca aacacgctgc acaaaaagaa
    10441 atgcttcctt taccaaaaac aagtttacaa atgatggatg ctgatctaaa tctcatcaca
    10501 acgcctaaac tatttttgga agaagaacag ctgccggaaa attatcgaat cgttggtcca
    10561 atttttgcta agctcagtac ttccttgcct gaagaagtgc taacttttat acagcgaaag
    10621 caacaagaaa gaaaacgtat tatctatttc gcaatgggta gttcaggcaa tccgcgtatg
    10681 ttacgcaaaa ttttgttctt cttacaaaaa caaccggatc tagcggttat tgcacctatt
    10741 gactatctgc ttgatgacat tcaatctgac tcagaagatc ttttaataac agaatatctg
    10801 tctagtcatc ttttgcatga attcattgat ttttctttta tccacggcgg ggaagggacc
    10861 gtccaaactg cctgtgaatc cggcaaaccg tttatcggtg tggggatgca atttgagcaa
    10921 agctgtaata ttgactattg tgtaaaatac ggaaacgcaa tcgccctcac aaaaccagtg
    10981 actgaaagaa aactgcagat tgctctgcgc gaagtttctt cacccaagat tcgtcagcag
    11041 gcacgcacgc tgcaagccgt ttttccaata aatggtccac aacaagcaat tcaagaaatc
    11101 gaaaccttcc tgagcaacca cccaatatag tttcaagtag aaaaagcttt tttaatagag
    11161 actcatcagt aatttcgcta ttcgagaccc agacagtgaa aatacaaaaa aagtaaatag
    11221 tggattgaaa agttagagtt acttaaatta cggtgtctaa aaaacacaaa atcatcctta
    11281 aaataaaaga tctgacgcta tccaaatgag cataggtagc gtctgccatt tattcttagt
    11341 caaattcttt gacacctttc tctggttgga tcatctcaac gtttcttcaa aagaaagacc
    11401 ataataatta ggcaaataca ttttcttaaa atgtgtcata tagatctaga agcatcgctt
    11461 tgtagagatt tgtgccgcta gtcccctcaa caattactaa atcaaatgta ggatttacca
    11521 ctttatcttt gataatcaac ctccctcttc tgttgagaca atgatctcgc aatccgatgc
    11581 atactcctat ataaactgcc agcaatcccc acatattgag tagatcatgt cagaatgttc
    11641 tatgtcgtca gcaacgatac actaaaagtc acgttcacca tgactgattg ctattcccag
    11701 cacaattatt tctatacacg tttcatgatt tgaataaaca ctcacatcat acatattacc
    11761 attcatacac catactgcag cccctactat atggttgtaa gctcccaaat ctatatttga
    11821 cttgatcact ttggtagtag atctactaat tctaaatcca ttgctgataa aatttccatt
    11881 ttttcctcct tctttctgcc aaaacagtta aaacaataat aatctttatg taaaaaaggg
    11941 ctgtgacaaa agccttgtca cagtcccata ttccgaataa acggtggtca aaagcttgca
    12001 tctgcggtaa taagttcaac caaacgaaaa ataagccaac atttcacaaa aatttaagaa
    12061 gcaattttcg taaatttctt cttatttctt gaagctaacc acttctgtta caaccacttt
    12121 tagaacattt aaagcttcga tgtgacaaac catctatgct gctcgatcat aaaattgata
    12181 gcaaaaattc cacaaagtac atatgtcccc catgaaaaca aaacattcat atgcggtaca
    12241 atgatctttg gaagaatcac gatcaaaaat gatgccagtg cccaaatcca tcgattgatt
    12301 tgaatatgat atgtagctag catattcaat ataaggtgac tagccgttaa taaaaagata
    12361 aaaacaaaaa ataaaatcag ccaatacatt gtttcccacc ttctaaaaac caaacttaga
    12421 cagtctggac ttcgttttct ccacgacttt gttcatctga cagcatcttt ttatcataag
    12481 cttttacaaa tggataatag ataataaatg ctatcaatgt gttgatcatg atcaagataa
    12541 caacacgcca gtcatttcct gctgaaagat aaccgccgat tggagctggt aatgtccatg
    12601 ttggcaaaac agtaaatcta ttgaccattt ctaatttgac tgcaaaataa ctgataatag
    12661 tcaaaactaa aggaactaag ttgaacggga tgaacatgta aggattcaac atcataggag
    12721 ctccaaaaat tatgggctca ttgatattga agataccagg aataatagaa aaacgaccaa
    12781 cctctttcaa gaattttgat ttacagaaga atataaacaa aacagatacc acgatcgttg
    12841 ctcctgcacc accaattgtt accgtccatt gataaaactg ttcaggagcg atatagggta
    12901 attgggtggc agctgtacca tctgccaatg cctttgcatt ctcatccaac atgatcagcc
    12961 acattggacg aacaacagcc cctacaatac tcatgccatg gataccaaat gaccacaaca
    13021 aatggatcaa aaacattggt agtaaaacac caagcaaatt attccctaaa atattggtta
    13081 aaggctcgaa taacgataat aaggcagcgt ttacatcaaa gcccgcgacg cttcgaatca
    13141 gccaagtaat tgttaaaatg actgcacctg gaatcaatgc ctcaaaacta cgggcaacag
    13201 caggtggcac ttgttctggc attttgatag taatatttcg atttttaaag aaacgatata
    13261 cttccactgc aaaaatcata gataagatcc ccgcaaacat cccagatgct cccaaagaac
    13321 tcataggtaa gacccagcca agaggtgcct cttcaggagt taaaacgttt acaggaactt
    13381 gtaacataaa gaacgttcct aaagataata taccacctgt cacactatct aatccatatg
    13441 atttagctag atccgaaccc ataacaaaag ttgcataaat agccatcaat cccatcgtca
    13501 tcctaaaggg taaaacaatc gttgccgaat aaggggcaat aaattcattc catccaggaa
    13561 acggtaaatt gataaagacc ataaaaaatg tcccaatcaa aatcaatgac aatgttgaaa
    13621 caaccccatt acgaattgct aataaatgac gctgtgtacc aatttttgcc attggtggga
    13681 caagtttctc ttcgacccat ttcatcagct tttccactaa aaccactcct tttcaataaa
    13741 atatgaacca ttcattgcat gacaacgcaa agaatgttca taactaatta tataattaag
    13801 aaattttatt tcaatatatt ctcttaaata atcaaattta tttttaagaa tcctgaaaat
    13861 aagttttcta aatcatagga gcaacaaaaa aattcgataa ttaacaaaaa tcaaaatgat
    13921 taaatctagt gattgaaaca tatcatttca aaaaaatttt gtaaattcac ccataaatat
    13981 caccctattt aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta
    14041 cttcttggtt ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca
    14101 ccgtatcttt ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt
    14161 cttttacctc ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg
    14221 tatctaaacc catatggatc agtatttttc tgccagattc actttcgatc gttatggcat
    14281 gtcgggtagg aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat
    14341 gattcgttat agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg
    14401 ctgttaatgg aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga
    14461 aatgcacttt ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca
    14521 tttcatctag tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg
    14581 cttgtaaagc tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg
    14641 caacttgtgc ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg
    14701 gctccacacc agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat
    14761 tcggaatcgc cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca
    14821 aggaaatagc tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt
    14881 cttccacaat accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat
    14941 ccatgaccat attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat
    15001 ataagtaata ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg
    15061 acatcatttg aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca
    15121 tttcttttgt tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat
    15181 cataaatcga tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct
    15241 tacgatcata accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg
    15301 cattaatcat ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg
    15361 gatttgtata attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt
    15421 ccaataaacc tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt
    15481 gtcctactaa tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc
    15541 caacacgaat ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg
    15601 ttgcacgaat gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt
    15661 acatatccat atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc
    15721 gtttttcatt gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag
    15781 ctatagtgat aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta
    15841 ttgaattctg ccataacata agtcacttct aagccgatga taatttgata atttttttca
    15901 gttggtttaa tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg
    15961 ggatccacta cctctacccg taggcgtgta ttacaactat agacatccag gatattggct
    16021 tttcctccaa acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac
    16081 cctaactctg tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct
    16141 aatgtctgaa tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa
    16201 aagaaaattc cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga
    16261 taatcaacaa tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca
    16321 atccctgcta aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg
    16381 aaactaaatt caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc
    16441 atgattcctt tgaccatctc tttattttct ttataagctg ctttatagat agctagacat
    16501 atagctggta taccaaacat cattactaca aaaaaacctg ataaaaacag tccagcctga
    16561 ggatctccgt tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta
    16621 tagcttccta aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc
    16681 agcaaacgat tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt
    16741 tttgcaaaat tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct
    16801 gcgattgttt gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa
    16861 aaaactactg gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg
    16921 gaaataattc caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt
    16981 ttcaaggtag caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg
    17041 acaaaaccga ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt
    17101 ccagctgcac caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat
    17161 cccgctaatg gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt
    17221 gatttaacat tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat
    17281 caaaggatca tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg
    17341 ggtacgagga tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg
    17401 acacatgact tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc
    17461 ttccacactc gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca
    17521 aggctcaaaa taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg
    17581 caaatcatat ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt
    17641 atgatgatga ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg
    17701 agcattccat tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga
    17761 taaggatctg aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc
    17821 acacgtcaat gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac
    17881 agcatgctcg aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg
    17941 ataagcatcc ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa
    18001 aatcactttt cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa
    18061 tcatagtcca tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt
    18121 aatagattct tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt
    18181 gaaattgctg ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt
    18241 taaatgtttc agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc
    18301 gtttaaccag gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat
    18361 aaacgtttga ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt
    18421 ttaagctctt taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga
    18481 atcctacttc atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt
    18541 ctactgctat atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg
    18601 tgacatctct atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa
    18661 tatagatatt tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt
    18721 ccgcaaaatc cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct
    18781 tttctttaga atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca
    18841 ctttagcaga aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga
    18901 tcaatacagc attatgattc agctttttca tcacatacac cgttaattca cctcctctat
    18961 atgataaaac aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt
    19021 taaagacaaa aaacgaccta agaaaga
//
LOCUS       DACETN010000048        18956 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DACETN010000048 DACETN010000000
VERSION     DACETN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.050"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(22..252)
                     /locus_tag="IXF98_001602"
     CDS             complement(22..252)
                     /locus_tag="IXF98_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555921.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQIDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IXF98_001603"
     CDS             complement(688..1254)
                     /locus_tag="IXF98_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1555922.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IXF98_001604"
     CDS             complement(1254..2210)
                     /locus_tag="IXF98_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1555923.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IXF98_001605"
     CDS             complement(2210..2866)
                     /locus_tag="IXF98_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1555924.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IXF98_001606"
     CDS             3236..5254
                     /locus_tag="IXF98_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1555925.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IXF98_001607"
     CDS             5681..8095
                     /locus_tag="IXF98_001607"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1555926.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IXF98_001608"
     CDS             8443..9996
                     /locus_tag="IXF98_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1555927.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IXF98_001609"
     CDS             complement(10377..12161)
                     /locus_tag="IXF98_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555928.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IXF98_001610"
     CDS             complement(12176..13897)
                     /locus_tag="IXF98_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1555929.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IXF98_001611"
                     /pseudo
     CDS             13997..14758
                     /locus_tag="IXF98_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            15725..15993
                     /locus_tag="IXF98_001612"
                     /pseudo
     CDS             15725..15993
                     /locus_tag="IXF98_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16665..>18956
                     /locus_tag="IXF98_001613"
     CDS             16665..>18956
                     /locus_tag="IXF98_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555930.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQ"
BASE COUNT         6231 a         3359 c         3802 g         5564 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatcaatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat gaagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatagg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    15361 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    15421 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg
    15481 gaaaaattca caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta
    15541 tgactaatat aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg
    15601 gaatcaatga aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat
    15661 agcaatacat cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac
    15721 aacaaaagga ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt
    15781 tattctatac aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa
    15841 tatatggtac agaatataga aattattgac attacagaaa atgatgaaga tttaatacag
    15901 attgtcacta tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg
    15961 attaaggagt tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga
    16021 gcaaaaagag taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt
    16081 ttattagtaa tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg
    16141 tttagttgaa ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc
    16201 caagtattct aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt
    16261 gatgaaggtt aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc
    16321 aacgatttag tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa
    16381 taactattag ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca
    16441 taattatggt taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac
    16501 agaagacaat cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa
    16561 ccgaaaatac gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta
    16621 tcgggtacct tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc
    16681 ataaactgtt aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa
    16741 cggttctagc tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc
    16801 aagaggaggc tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc
    16861 taaatgcatt ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca
    16921 atgattacga acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg
    16981 caagaagcgg tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa
    17041 gcacaagtga gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag
    17101 aagaaagctt aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg
    17161 aatctcagat tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc
    17221 gccccacttt gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg
    17281 gcgatattct tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct
    17341 taattgagtt cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt
    17401 accaagaagg cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag
    17461 ccgctcagtc gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta
    17521 ctgagccaaa taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg
    17581 aattttcaat tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct
    17641 ctagagaaat cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga
    17701 ccaacagtaa taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag
    17761 tggttaccga tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt
    17821 ccactggaaa tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag
    17881 gtgaagcaaa cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta
    17941 ataaggaaac gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt
    18001 ttattgaata tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg
    18061 atgggttttc tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact
    18121 accgcttgag aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca
    18181 aaaatgttat caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt
    18241 ctggaacaag aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt
    18301 catttctatc agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt
    18361 tcagtgcgaa taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg
    18421 tagaggcgta tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca
    18481 atcgattgaa tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta
    18541 aaactgatga gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt
    18601 cacaagtgat acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc
    18661 ctgtaggtag ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga
    18721 cggtacatga tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa
    18781 acatccttga tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc
    18841 ttcaaggagc tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtttaa
    18901 ctacaggaga agatggaaaa ctggcaattg acggtttagc accaggagca tatcag
//
LOCUS       DACETN010000049        18534 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DACETN010000049 DACETN010000000
VERSION     DACETN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.051"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            129..776
                     /locus_tag="IXF98_001614"
     CDS             129..776
                     /locus_tag="IXF98_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1555931.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1169..3814
                     /locus_tag="IXF98_001615"
     CDS             1169..3814
                     /locus_tag="IXF98_001615"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1555932.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4124..5437
                     /locus_tag="IXF98_001616"
     CDS             4124..5437
                     /locus_tag="IXF98_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1555933.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5427..6080
                     /locus_tag="IXF98_001617"
     CDS             5427..6080
                     /locus_tag="IXF98_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1555934.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6135..6827
                     /locus_tag="IXF98_001618"
     CDS             6135..6827
                     /locus_tag="IXF98_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1555935.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6977..7177
                     /locus_tag="IXF98_001619"
     CDS             6977..7177
                     /locus_tag="IXF98_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1555936.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7431..9110)
                     /locus_tag="IXF98_001620"
     CDS             complement(7431..9110)
                     /locus_tag="IXF98_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1555937.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9112..9324)
                     /locus_tag="IXF98_001621"
     CDS             complement(9112..9324)
                     /locus_tag="IXF98_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1555938.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9717..11447
                     /gene="efrA"
                     /locus_tag="IXF98_001622"
     CDS             9717..11447
                     /gene="efrA"
                     /locus_tag="IXF98_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1555939.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11444..13204
                     /gene="efrB"
                     /locus_tag="IXF98_001623"
     CDS             11444..13204
                     /gene="efrB"
                     /locus_tag="IXF98_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1555940.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13299..13496
                     /locus_tag="IXF98_001624"
     CDS             13299..13496
                     /locus_tag="IXF98_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555941.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13649..14134
                     /locus_tag="IXF98_001625"
     CDS             13649..14134
                     /locus_tag="IXF98_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1555942.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14238..14831)
                     /gene="clpP"
                     /locus_tag="IXF98_001626"
     CDS             complement(14238..14831)
                     /gene="clpP"
                     /locus_tag="IXF98_001626"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1555943.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15011..15538
                     /locus_tag="IXF98_001627"
     CDS             15011..15538
                     /locus_tag="IXF98_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1555944.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15629..16366
                     /locus_tag="IXF98_001628"
     CDS             15629..16366
                     /locus_tag="IXF98_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1555945.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16370..17287
                     /gene="miaA"
                     /locus_tag="IXF98_001629"
     CDS             16370..17287
                     /gene="miaA"
                     /locus_tag="IXF98_001629"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1555946.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17289..18518
                     /gene="hflX"
                     /locus_tag="IXF98_001630"
     CDS             17289..18518
                     /gene="hflX"
                     /locus_tag="IXF98_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1555947.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5991 a         3208 c         4028 g         5307 t
ORIGIN      
        1 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
       61 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
      121 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
      181 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
      241 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
      301 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
      361 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
      421 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
      481 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
      541 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
      601 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
      661 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
      721 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
      781 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
      841 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
      901 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
      961 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     1021 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     1081 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     1141 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     1201 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
     1261 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
     1321 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
     1381 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
     1441 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
     1501 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
     1561 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
     1621 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
     1681 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
     1741 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
     1801 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
     1861 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
     1921 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
     1981 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
     2041 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
     2101 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
     2161 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
     2221 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
     2281 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
     2341 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
     2401 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
     2461 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
     2521 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
     2581 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
     2641 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
     2701 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
     2761 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
     2821 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
     2881 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
     2941 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
     3001 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
     3061 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
     3121 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
     3181 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
     3241 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
     3301 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
     3361 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
     3421 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
     3481 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
     3541 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
     3601 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
     3661 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
     3721 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
     3781 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
     3841 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
     3901 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
     3961 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
     4021 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
     4081 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
     4141 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
     4201 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
     4261 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
     4321 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
     4381 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
     4441 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
     4501 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
     4561 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
     4621 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
     4681 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
     4741 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
     4801 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
     4861 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
     4921 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
     4981 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
     5041 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
     5101 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
     5161 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
     5221 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
     5281 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
     5341 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
     5401 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
     5461 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
     5521 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
     5581 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
     5641 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
     5701 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
     5761 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
     5821 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
     5881 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
     5941 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
     6001 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
     6061 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
     6121 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
     6181 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
     6241 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
     6301 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
     6361 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
     6421 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
     6481 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
     6541 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
     6601 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
     6661 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
     6721 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
     6781 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
     6841 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
     6901 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
     6961 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
     7021 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
     7081 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
     7141 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
     7201 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
     7261 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
     7321 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
     7381 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
     7441 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
     7501 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
     7561 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
     7621 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
     7681 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
     7741 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
     7801 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
     7861 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
     7921 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
     7981 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
     8041 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
     8101 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
     8161 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
     8221 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
     8281 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
     8341 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
     8401 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
     8461 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
     8521 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
     8581 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
     8641 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
     8701 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
     8761 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
     8821 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
     8881 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
     8941 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
     9001 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
     9061 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
     9121 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
     9181 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
     9241 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
     9301 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
     9361 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
     9421 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
     9481 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
     9541 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
     9601 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
     9661 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
     9721 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
     9781 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
     9841 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
     9901 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
     9961 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    10021 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    10081 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    10141 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    10201 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    10261 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    10321 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    10381 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    10441 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    10501 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    10561 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    10621 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    10681 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    10741 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    10801 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    10861 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    10921 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    10981 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    11041 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    11101 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    11161 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    11221 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    11281 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    11341 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    11401 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    11461 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    11521 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    11581 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    11641 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    11701 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    11761 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    11821 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    11881 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    11941 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    12001 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    12061 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    12121 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    12181 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    12241 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    12301 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    12361 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    12421 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    12481 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    12541 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    12601 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    12661 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    12721 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    12781 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    12841 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    12901 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    12961 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    13021 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    13081 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    13141 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    13201 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    13261 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    13321 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    13381 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    13441 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    13501 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    13561 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    13621 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    13681 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    13741 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    13801 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    13861 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    13921 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    13981 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    14041 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    14101 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    14161 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    14221 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    14281 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    14341 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    14401 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    14461 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    14521 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    14581 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    14641 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    14701 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    14761 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    14821 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    14881 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    14941 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    15001 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    15061 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    15121 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    15181 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    15241 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    15301 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    15361 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    15421 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    15481 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    15541 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    15601 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    15661 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    15721 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    15781 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    15841 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    15901 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    15961 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    16021 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    16081 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    16141 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    16201 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    16261 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    16321 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    16381 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    16441 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    16501 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    16561 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    16621 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    16681 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    16741 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    16801 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    16861 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    16921 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    16981 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    17041 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    17101 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    17161 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    17221 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    17281 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    17341 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    17401 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    17461 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    17521 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    17581 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    17641 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    17701 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    17761 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    17821 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    17881 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    17941 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    18001 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    18061 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    18121 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    18181 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    18241 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    18301 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    18361 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    18421 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    18481 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DACETN010000050        17687 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DACETN010000050 DACETN010000000
VERSION     DACETN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.052"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(136..1716)
                     /locus_tag="IXF98_001631"
     CDS             complement(136..1716)
                     /locus_tag="IXF98_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1555948.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IXF98_001632"
     CDS             complement(2117..3493)
                     /locus_tag="IXF98_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1555949.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IXF98_001633"
     CDS             complement(3621..4739)
                     /locus_tag="IXF98_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1555950.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IXF98_001634"
     CDS             4881..5315
                     /locus_tag="IXF98_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1555951.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IXF98_001635"
     CDS             complement(5393..5926)
                     /locus_tag="IXF98_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1555952.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IXF98_001636"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IXF98_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1555953.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IXF98_001637"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IXF98_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1555954.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IXF98_001638"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IXF98_001638"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1555955.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IXF98_001639"
     CDS             complement(9651..10229)
                     /locus_tag="IXF98_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1555956.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IXF98_001640"
     CDS             10551..11021
                     /locus_tag="IXF98_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1555957.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IXF98_001641"
     CDS             complement(11129..12355)
                     /locus_tag="IXF98_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1555958.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="IXF98_001642"
     CDS             complement(13109..13309)
                     /locus_tag="IXF98_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1555959.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="IXF98_001643"
     CDS             complement(13443..14051)
                     /locus_tag="IXF98_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1555960.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="IXF98_001644"
     CDS             complement(14136..14606)
                     /locus_tag="IXF98_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1555961.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="IXF98_001645"
     CDS             complement(14654..15568)
                     /locus_tag="IXF98_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1555962.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="IXF98_001646"
     CDS             15714..16517
                     /locus_tag="IXF98_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1555963.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="IXF98_001647"
     CDS             16695..17642
                     /locus_tag="IXF98_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1555964.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5363 a         3543 c         2910 g         5871 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DACETN010000051        17517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DACETN010000051 DACETN010000000
VERSION     DACETN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.053"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            100..2427
                     /locus_tag="IXF98_001648"
     CDS             100..2427
                     /locus_tag="IXF98_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1555965.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IXF98_001649"
     CDS             2553..2882
                     /locus_tag="IXF98_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1555966.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IXF98_001650"
     CDS             2922..3926
                     /locus_tag="IXF98_001650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1555967.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IXF98_001651"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IXF98_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IXF98_001652"
     CDS             5558..6730
                     /locus_tag="IXF98_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1555968.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IXF98_001653"
     CDS             complement(6892..7347)
                     /locus_tag="IXF98_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555969.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IXF98_001654"
     CDS             7936..8358
                     /locus_tag="IXF98_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1555970.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IXF98_001655"
     CDS             complement(8524..9717)
                     /locus_tag="IXF98_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1555971.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IXF98_001656"
     CDS             9941..10318
                     /locus_tag="IXF98_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1555972.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IXF98_001657"
     CDS             10306..10644
                     /locus_tag="IXF98_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1555973.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IXF98_001658"
     CDS             10778..11221
                     /locus_tag="IXF98_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1555974.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IXF98_001659"
     CDS             11483..12298
                     /locus_tag="IXF98_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1555975.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IXF98_001660"
     CDS             12308..12970
                     /locus_tag="IXF98_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1555976.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IXF98_001661"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IXF98_001661"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1555977.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IXF98_001662"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IXF98_001662"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1555978.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IXF98_001663"
     CDS             15692..16189
                     /locus_tag="IXF98_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1555979.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IXF98_001664"
     CDS             16374..16634
                     /locus_tag="IXF98_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555980.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IXF98_001665"
     CDS             complement(16996..17235)
                     /locus_tag="IXF98_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1555981.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3474 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggatag
//
LOCUS       DACETN010000052        17180 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DACETN010000052 DACETN010000000
VERSION     DACETN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.054"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(543..2048)
                     /locus_tag="IXF98_001666"
     CDS             complement(543..2048)
                     /locus_tag="IXF98_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1555982.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IXF98_001667"
     CDS             complement(2045..2731)
                     /locus_tag="IXF98_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1555983.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXF98_001668"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXF98_001668"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1555984.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IXF98_001669"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IXF98_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1555985.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IXF98_001670"
     CDS             complement(4752..5321)
                     /locus_tag="IXF98_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1555986.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IXF98_001671"
     CDS             5507..6409
                     /locus_tag="IXF98_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1555987.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IXF98_001672"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IXF98_001672"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1555988.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IXF98_001673"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IXF98_001673"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1555989.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IXF98_001674"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IXF98_001674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1555990.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IXF98_001675"
     CDS             12977..13162
                     /locus_tag="IXF98_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1555991.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IXF98_001676"
     CDS             complement(13224..14420)
                     /locus_tag="IXF98_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1555992.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IXF98_001677"
     CDS             complement(14590..16026)
                     /locus_tag="IXF98_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1555993.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IXF98_001678"
     CDS             16319..17128
                     /locus_tag="IXF98_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1555994.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5042 a         3595 c         2983 g         5560 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct
//
LOCUS       DACETN010000053        17136 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DACETN010000053 DACETN010000000
VERSION     DACETN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.055"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(151..2058)
                     /locus_tag="IXF98_001679"
     CDS             complement(151..2058)
                     /locus_tag="IXF98_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1555995.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IXF98_001680"
     CDS             2188..3063
                     /locus_tag="IXF98_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1555996.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IXF98_001681"
     CDS             3143..4117
                     /locus_tag="IXF98_001681"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1555997.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IXF98_001682"
     CDS             4306..5442
                     /locus_tag="IXF98_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1555998.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IXF98_001683"
     CDS             5579..5923
                     /locus_tag="IXF98_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1555999.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IXF98_001684"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IXF98_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1556000.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IXF98_001685"
     CDS             complement(7618..8217)
                     /locus_tag="IXF98_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1556001.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IXF98_001686"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IXF98_001686"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1556002.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IXF98_001687"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IXF98_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1556003.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IXF98_001688"
     CDS             11423..12541
                     /locus_tag="IXF98_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1556004.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IXF98_001689"
     CDS             complement(12601..13893)
                     /locus_tag="IXF98_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1556005.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="IXF98_001690"
     CDS             14043..14603
                     /locus_tag="IXF98_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1556006.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IXF98_001691"
     CDS             14732..15109
                     /locus_tag="IXF98_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1556007.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IXF98_001692"
     CDS             15042..17114
                     /locus_tag="IXF98_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1556008.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5472 a         3105 c         3652 g         4907 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagcgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caatgataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc acgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DACETN010000054        17108 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DACETN010000054 DACETN010000000
VERSION     DACETN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.056"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..379
                     /locus_tag="IXF98_001693"
     CDS             <1..379
                     /locus_tag="IXF98_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1556009.1"
                     /translation="EVDPEEKPDVTPEPDTDARDQGIPEKINKKTIQEDGKKESKKSN
                     LAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQLPKSGESQNKVILW
                     SGIILLSIATMLSAKRFKQNRSL"
     gene            complement(505..1584)
                     /locus_tag="IXF98_001694"
     CDS             complement(505..1584)
                     /locus_tag="IXF98_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1556010.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1693..2133)
                     /locus_tag="IXF98_001695"
     CDS             complement(1693..2133)
                     /locus_tag="IXF98_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1556011.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2190..2600)
                     /locus_tag="IXF98_001696"
     CDS             complement(2190..2600)
                     /locus_tag="IXF98_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1556012.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2781..3215)
                     /locus_tag="IXF98_001697"
     CDS             complement(2781..3215)
                     /locus_tag="IXF98_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1556013.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(3315..4163)
                     /locus_tag="IXF98_001698"
     CDS             complement(3315..4163)
                     /locus_tag="IXF98_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1556014.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4552..6318
                     /locus_tag="IXF98_001699"
     CDS             4552..6318
                     /locus_tag="IXF98_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1556015.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6420..7679
                     /locus_tag="IXF98_001700"
     CDS             6420..7679
                     /locus_tag="IXF98_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1556016.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7826..9127
                     /locus_tag="IXF98_001701"
     CDS             7826..9127
                     /locus_tag="IXF98_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1556017.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9130..9981
                     /locus_tag="IXF98_001702"
     CDS             9130..9981
                     /locus_tag="IXF98_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1556018.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9994..10809
                     /locus_tag="IXF98_001703"
     CDS             9994..10809
                     /locus_tag="IXF98_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1556019.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10960..12126
                     /locus_tag="IXF98_001704"
     CDS             10960..12126
                     /locus_tag="IXF98_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1556020.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12356..13003
                     /locus_tag="IXF98_001705"
     CDS             12356..13003
                     /locus_tag="IXF98_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1556021.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13087..14031
                     /locus_tag="IXF98_001706"
     CDS             13087..14031
                     /locus_tag="IXF98_001706"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1556022.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14166..15617
                     /locus_tag="IXF98_001707"
     CDS             14166..15617
                     /locus_tag="IXF98_001707"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1556023.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15638..16474
                     /locus_tag="IXF98_001708"
     CDS             15638..16474
                     /locus_tag="IXF98_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1556024.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16474..>17108
                     /locus_tag="IXF98_001709"
     CDS             16474..>17108
                     /locus_tag="IXF98_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1556025.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLE"
BASE COUNT         5708 a         3029 c         3330 g         5041 t
ORIGIN      
        1 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
       61 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
      121 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
      181 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
      241 tagtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat
      301 actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa
      361 acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag
      421 aaatatccag aaaacgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat
      481 actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg
      541 gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa
      601 gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg
      661 cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac
      721 aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat
      781 gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa
      841 aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt
      901 gagcaacgtg tttaaaagag cgataacaaa ttgagcttct aataccaaac caaatgtatt
      961 gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa
     1021 aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat
     1081 gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat
     1141 cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata
     1201 ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaatcaataa cggactggat
     1261 catatcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag
     1321 gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg
     1381 ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt
     1441 tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg
     1501 gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata
     1561 acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa agagaaagag
     1621 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     1681 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     1741 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     1801 tggaatacct tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg taacatttac
     1861 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     1921 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     1981 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     2041 cggcttgagg agggaagtgt agtgtctaat gatttcctta gaggcggcat ataaaggaaa
     2101 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     2161 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     2221 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     2281 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     2341 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     2401 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     2461 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     2521 tcgaatgtac gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt gatcattttc
     2581 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     2641 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     2701 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat cagagacaaa
     2761 aactaatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa
     2821 cttacttcac gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt
     2881 gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt
     2941 cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta
     3001 tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg
     3061 aatctcgtgc catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc
     3121 tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca
     3181 tttaatacaa aatctatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg
     3241 aacagttact agattctttt attataccag ttaaagaaaa cgcattcaaa aaaatagcgg
     3301 atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct
     3361 acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg
     3421 gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg
     3481 ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg
     3541 tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg
     3601 atataagtca tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct
     3661 attccaaaaa caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc
     3721 gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta
     3781 gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg
     3841 acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga
     3901 aaaaacaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct
     3961 agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa tcctaaaaca
     4021 gcttccagta atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa
     4081 aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat
     4141 ataaattgtc tcgtttcttt catttatcca cttccttaat tcattaataa tactcttatc
     4201 ttattataac ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc
     4261 taaattatta tgtttcattc ttctagaata tacttctacg ttaaaaactg ttaaaaaact
     4321 tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta
     4381 caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa
     4441 taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt
     4501 tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca
     4561 gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt
     4621 cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat
     4681 ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta
     4741 agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag
     4801 tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt
     4861 tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt
     4921 catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt
     4981 tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg
     5041 ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat
     5101 catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa
     5161 gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc
     5221 gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg
     5281 ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa
     5341 aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct
     5401 tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt
     5461 actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat
     5521 atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat
     5581 gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac
     5641 atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc
     5701 catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa
     5761 atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag
     5821 gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt
     5881 tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg
     5941 tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc
     6001 cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa
     6061 gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg
     6121 ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga gggacagatc
     6181 atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg
     6241 attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga
     6301 tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag
     6361 atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga
     6421 tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg
     6481 gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa
     6541 cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt
     6601 tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg
     6661 aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg
     6721 accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag
     6781 cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac
     6841 ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat
     6901 caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag
     6961 caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa
     7021 ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt
     7081 tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt
     7141 ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt
     7201 ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca
     7261 atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt
     7321 ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa
     7381 aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg
     7441 aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa
     7501 cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg
     7561 aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa
     7621 aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat
     7681 taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca
     7741 agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt
     7801 tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa
     7861 aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt
     7921 taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg
     7981 atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt
     8041 atttattttg attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta
     8101 cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat
     8161 caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt
     8221 gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt
     8281 gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg
     8341 ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt
     8401 tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct
     8461 tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg
     8521 tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa
     8581 tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct
     8641 tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc
     8701 aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat
     8761 cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg
     8821 gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat
     8881 tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta acaatggtgg
     8941 tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa
     9001 acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc
     9061 cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg
     9121 ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt
     9181 tttacttact tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca
     9241 gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac
     9301 tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa
     9361 aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg
     9421 ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta
     9481 gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta
     9541 ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca
     9601 atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct
     9661 gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc
     9721 aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg
     9781 ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca
     9841 tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt
     9901 gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca
     9961 tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt
    10021 tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa
    10081 tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat
    10141 gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat
    10201 gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg
    10261 caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa
    10321 caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag
    10381 gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca accagattct
    10441 gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt
    10501 ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt
    10561 ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc
    10621 attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg
    10681 gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca
    10741 acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt
    10801 gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa
    10861 gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag
    10921 tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac
    10981 aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca
    11041 acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa
    11101 atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat
    11161 catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca
    11221 aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg
    11281 aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa
    11341 ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg
    11401 ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa
    11461 ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa
    11521 gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc
    11581 aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc
    11641 gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt
    11701 tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca
    11761 gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa
    11821 tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa
    11881 aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa
    11941 cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc
    12001 ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat
    12061 atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt
    12121 gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat
    12181 gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt
    12241 ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt
    12301 tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa
    12361 aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa
    12421 taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt
    12481 atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga
    12541 agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga
    12601 taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt
    12661 ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa
    12721 tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa
    12781 gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat
    12841 cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg
    12901 tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa
    12961 tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac
    13021 aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg
    13081 atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat
    13141 gcatctggtg tccactgcat gactacccag gaattagatg aactggctca ttcagaagca
    13201 ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc
    13261 tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct
    13321 tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta
    13381 tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa
    13441 aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa
    13501 ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc
    13561 ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag
    13621 atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc
    13681 aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc
    13741 ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat
    13801 acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat
    13861 gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa
    13921 gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa
    13981 ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac
    14041 gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg
    14101 caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg
    14161 ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc
    14221 aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt
    14281 ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa
    14341 aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat
    14401 ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt
    14461 atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt
    14521 ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc
    14581 ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg
    14641 ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg
    14701 gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag
    14761 gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt
    14821 tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt
    14881 ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc
    14941 actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa
    15001 aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt
    15061 cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca
    15121 tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg
    15181 gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa
    15241 ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta
    15301 ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca
    15361 tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct
    15421 tgaagatcac accttataca cagttggcta atctgactgg acaaccagca atcagcttgc
    15481 ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa
    15541 gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac
    15601 caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc
    15661 tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga
    15721 agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac
    15781 actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt
    15841 ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga
    15901 tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat
    15961 cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag
    16021 tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa
    16081 aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa
    16141 attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg
    16201 aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga
    16261 tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt
    16321 gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga
    16381 agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc
    16441 gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc
    16501 ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc
    16561 ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg
    16621 gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc
    16681 gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg
    16741 gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga
    16801 tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc
    16861 tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc
    16921 agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca
    16981 gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat
    17041 attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat
    17101 ttagaaaa
//
LOCUS       DACETN010000055        17074 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DACETN010000055 DACETN010000000
VERSION     DACETN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.057"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(9..530)
                     /locus_tag="IXF98_001710"
     CDS             complement(9..530)
                     /locus_tag="IXF98_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1556026.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IXF98_001711"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IXF98_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1556027.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IXF98_001712"
     CDS             4610..5497
                     /locus_tag="IXF98_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1556028.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IXF98_001713"
     CDS             5494..6663
                     /locus_tag="IXF98_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556029.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IXF98_001714"
     CDS             6687..8042
                     /locus_tag="IXF98_001714"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1556030.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IXF98_001715"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IXF98_001715"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1556031.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="IXF98_001716"
     CDS             10328..10618
                     /locus_tag="IXF98_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556032.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="IXF98_001717"
                     /pseudo
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="IXF98_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
     gene            11659..12318
                     /locus_tag="IXF98_001718"
     CDS             11659..12318
                     /locus_tag="IXF98_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1556033.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="IXF98_001719"
     CDS             12475..13407
                     /locus_tag="IXF98_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556034.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /locus_tag="IXF98_001720"
     CDS             13561..14850
                     /locus_tag="IXF98_001720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1556035.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="IXF98_001721"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="IXF98_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1556036.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /locus_tag="IXF98_001722"
     CDS             16430..17017
                     /locus_tag="IXF98_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1556037.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5585 a         3061 c         3739 g         4689 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtataat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agtc
//
LOCUS       DACETN010000056        17008 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DACETN010000056 DACETN010000000
VERSION     DACETN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.058"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..126)
                     /locus_tag="IXF98_001723"
     CDS             complement(<1..126)
                     /locus_tag="IXF98_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1556038.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="IXF98_001724"
     CDS             complement(230..541)
                     /locus_tag="IXF98_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1556039.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="IXF98_001725"
     CDS             complement(543..740)
                     /locus_tag="IXF98_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1556040.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="IXF98_001726"
     CDS             complement(1134..2861)
                     /locus_tag="IXF98_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1556041.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="IXF98_001727"
     CDS             complement(2854..4047)
                     /locus_tag="IXF98_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1556042.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IXF98_001728"
     CDS             complement(4235..4879)
                     /locus_tag="IXF98_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1556043.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IXF98_001729"
     CDS             complement(4973..5107)
                     /locus_tag="IXF98_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1556044.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IXF98_001730"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IXF98_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1556045.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IXF98_001731"
     CDS             complement(7238..7711)
                     /locus_tag="IXF98_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1556046.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IXF98_001732"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IXF98_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1556047.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IXF98_001733"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IXF98_001733"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1556048.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IXF98_001734"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IXF98_001734"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1556049.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IXF98_001735"
     CDS             complement(11758..12897)
                     /locus_tag="IXF98_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1556050.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IXF98_001736"
     CDS             complement(13206..13919)
                     /locus_tag="IXF98_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1556051.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IXF98_001737"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IXF98_001737"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1556052.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IXF98_001738"
     CDS             15108..16640
                     /locus_tag="IXF98_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1556053.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="IXF98_001739"
     CDS             complement(16831..>17008)
                     /locus_tag="IXF98_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1556054.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3496 c         2909 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtcgtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DACETN010000057        16631 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DACETN010000057 DACETN010000000
VERSION     DACETN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.059"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(45..779)
                     /locus_tag="IXF98_001740"
     CDS             complement(45..779)
                     /locus_tag="IXF98_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1556055.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IXF98_001741"
     CDS             1105..1515
                     /locus_tag="IXF98_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1556056.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IXF98_001742"
     CDS             1508..2209
                     /locus_tag="IXF98_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1556057.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IXF98_001743"
     CDS             2209..3822
                     /locus_tag="IXF98_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1556058.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IXF98_001744"
     CDS             3812..4033
                     /locus_tag="IXF98_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1556059.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IXF98_001745"
     CDS             4030..4791
                     /locus_tag="IXF98_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1556060.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IXF98_001746"
     CDS             5172..5681
                     /locus_tag="IXF98_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1556061.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IXF98_001747"
     CDS             complement(5751..6290)
                     /locus_tag="IXF98_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1556062.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IXF98_001748"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IXF98_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1556063.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IXF98_001749"
     CDS             7357..8154
                     /locus_tag="IXF98_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1556064.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IXF98_001750"
     CDS             8182..8817
                     /locus_tag="IXF98_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1556065.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IXF98_001751"
     CDS             8837..9610
                     /locus_tag="IXF98_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1556066.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IXF98_001752"
     CDS             10018..10815
                     /locus_tag="IXF98_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1556067.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IXF98_001753"
     CDS             10793..11206
                     /locus_tag="IXF98_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556068.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IXF98_001754"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IXF98_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1556069.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IXF98_001755"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IXF98_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1556070.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IXF98_001756"
     CDS             15983..16543
                     /locus_tag="IXF98_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1556071.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5399 a         2969 c         3602 g         4661 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    16621 actctgacta a
//
LOCUS       DACETN010000058        16611 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DACETN010000058 DACETN010000000
VERSION     DACETN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.060"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            248..1123
                     /locus_tag="IXF98_001757"
     CDS             248..1123
                     /locus_tag="IXF98_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1556072.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(1275..2117)
                     /locus_tag="IXF98_001758"
     CDS             complement(1275..2117)
                     /locus_tag="IXF98_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1556073.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(2209..4086)
                     /locus_tag="IXF98_001759"
     CDS             complement(2209..4086)
                     /locus_tag="IXF98_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1556074.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(4083..5504)
                     /locus_tag="IXF98_001760"
     CDS             complement(4083..5504)
                     /locus_tag="IXF98_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1556075.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(5507..8896)
                     /locus_tag="IXF98_001761"
     CDS             complement(5507..8896)
                     /locus_tag="IXF98_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1556076.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            9153..10544
                     /locus_tag="IXF98_001762"
     CDS             9153..10544
                     /locus_tag="IXF98_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1556077.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(10617..11264)
                     /locus_tag="IXF98_001763"
     CDS             complement(10617..11264)
                     /locus_tag="IXF98_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1556078.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(11272..11958)
                     /locus_tag="IXF98_001764"
     CDS             complement(11272..11958)
                     /locus_tag="IXF98_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556079.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(11964..13037)
                     /locus_tag="IXF98_001765"
     CDS             complement(11964..13037)
                     /locus_tag="IXF98_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1556080.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            13259..13933
                     /locus_tag="IXF98_001766"
     CDS             13259..13933
                     /locus_tag="IXF98_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1556081.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            13938..15278
                     /locus_tag="IXF98_001767"
     CDS             13938..15278
                     /locus_tag="IXF98_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1556082.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(15427..16413)
                     /locus_tag="IXF98_001768"
     CDS             complement(15427..16413)
                     /locus_tag="IXF98_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1556083.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         4751 a         3099 c         3044 g         5717 t
ORIGIN      
        1 tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat
       61 tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa
      121 aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg
      181 ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg
      241 tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa
      301 acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga
      361 attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg
      421 attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc
      481 tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca
      541 aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat
      601 tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct
      661 ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt
      721 gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt
      781 gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg
      841 aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca
      901 ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt
      961 tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt
     1021 ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga
     1081 attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta
     1141 gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa
     1201 gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata
     1261 gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata
     1321 agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc
     1381 tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct
     1441 ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa
     1501 tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga
     1561 tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc
     1621 agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg
     1681 gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt
     1741 tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc
     1801 gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt
     1861 ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc
     1921 tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc
     1981 tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga
     2041 taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc
     2101 cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag
     2161 gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact
     2221 gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat
     2281 atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat
     2341 tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc
     2401 tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt
     2461 gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc
     2521 tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt
     2581 cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga
     2641 tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg
     2701 tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt
     2761 tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt
     2821 taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac
     2881 agaataattt actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc
     2941 tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt
     3001 gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc
     3061 atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc
     3121 aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat
     3181 ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc
     3241 ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga
     3301 tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc
     3361 tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt
     3421 gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt
     3481 ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg
     3541 gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt
     3601 gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt
     3661 tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa
     3721 agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga
     3781 ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc
     3841 ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc
     3901 gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt
     3961 atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt
     4021 tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt
     4081 tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat
     4141 accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga
     4201 cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa
     4261 agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct
     4321 tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt
     4381 ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct
     4441 gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt
     4501 ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact
     4561 ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc
     4621 gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat
     4681 ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca
     4741 aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt
     4801 aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga
     4861 attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct
     4921 tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa
     4981 tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc
     5041 tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct
     5101 cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga
     5161 gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct
     5221 gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca
     5281 tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct
     5341 tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa
     5401 gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat
     5461 agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc
     5521 tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat
     5581 agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga
     5641 tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc
     5701 catccacttg aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt
     5761 ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga
     5821 atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct
     5881 gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta
     5941 gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg
     6001 tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg
     6061 gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga
     6121 gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag
     6181 taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt
     6241 ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa
     6301 tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac
     6361 catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag
     6421 cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag
     6481 aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga
     6541 aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta
     6601 acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc
     6661 ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt
     6721 gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt
     6781 ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt
     6841 ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg
     6901 gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc
     6961 cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct
     7021 ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg
     7081 tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat
     7141 tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc
     7201 ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat
     7261 ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag
     7321 agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa
     7381 ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt
     7441 gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc
     7501 cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat
     7561 catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag
     7621 ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg
     7681 gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc
     7741 ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa
     7801 tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt
     7861 agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc
     7921 tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat
     7981 tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt
     8041 tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct
     8101 ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg
     8161 aatgggtccg atcattagga tctccattcc aagaatttat accatcccat tgatttgttc
     8221 cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt
     8281 taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca
     8341 cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt
     8401 cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct
     8461 tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct
     8521 ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg
     8581 atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt
     8641 ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag
     8701 cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa
     8761 tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag
     8821 ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct
     8881 tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg
     8941 aaaattatta aaataaacag gcgttacata attttattta ttttaatatg ataattatta
     9001 tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag
     9061 ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata
     9121 tgatcaggtt gttggaatta aactatagaa agatggaaaa aatcattcga attctgtcca
     9181 tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta
     9241 catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat
     9301 cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc
     9361 tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc
     9421 cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac
     9481 gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc
     9541 aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt
     9601 tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca
     9661 aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg
     9721 agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc
     9781 catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact
     9841 tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga
     9901 actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg
     9961 agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa
    10021 acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat
    10081 acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc
    10141 agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc
    10201 agaatctgat ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa
    10261 ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata
    10321 gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga
    10381 caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg
    10441 ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt
    10501 cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc
    10561 tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat
    10621 tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt
    10681 gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat
    10741 aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc
    10801 agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga
    10861 tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg
    10921 cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg
    10981 aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca
    11041 tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg
    11101 atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca
    11161 tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc
    11221 tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt
    11281 ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg
    11341 aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt
    11401 aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc
    11461 tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc
    11521 tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag
    11581 caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa
    11641 ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc
    11701 tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat
    11761 cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt
    11821 acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat
    11881 tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat
    11941 cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt
    12001 atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat
    12061 agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc
    12121 aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct
    12181 actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata
    12241 gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag
    12301 aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt
    12361 tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt
    12421 ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc
    12481 tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact
    12541 gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt
    12601 ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca
    12661 ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt
    12721 gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg
    12781 attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct
    12841 gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt
    12901 gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac
    12961 gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct
    13021 ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca
    13081 cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc
    13141 aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt
    13201 aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat
    13261 gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact
    13321 cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga
    13381 aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga
    13441 agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa
    13501 agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa
    13561 acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg
    13621 tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga
    13681 agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta
    13741 ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg
    13801 atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca
    13861 tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact
    13921 gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg
    13981 cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt actttacaga
    14041 tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt
    14101 ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga
    14161 ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc
    14221 gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta
    14281 ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca
    14341 tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta
    14401 cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa
    14461 atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt
    14521 tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta
    14581 ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac
    14641 cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat catactatta
    14701 ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga
    14761 cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa
    14821 tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca
    14881 tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa
    14941 aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag
    15001 aagataagat cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga
    15061 atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg
    15121 aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac
    15181 aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg
    15241 attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct
    15301 gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc
    15361 ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc
    15421 ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc
    15481 caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg
    15541 aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat
    15601 tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt
    15661 tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt
    15721 atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac
    15781 atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat
    15841 gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt
    15901 tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc
    15961 cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac
    16021 agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt
    16081 gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg
    16141 tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa
    16201 agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa
    16261 tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg
    16321 ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata
    16381 gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta
    16441 tgattggtat attgttactt ataaatttat tctataggag acgattgttt tctacaaata
    16501 tttagattgg aatttgatta aaacaattta attgaaattt gaaaacattt atgttaatga
    16561 aagtcatatt ctaataaaat gtcatttgtt tctatatgaa tttttatgaa a
//
LOCUS       DACETN010000059        16536 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DACETN010000059 DACETN010000000
VERSION     DACETN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.061"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            746..1786
                     /locus_tag="IXF98_001769"
     CDS             746..1786
                     /locus_tag="IXF98_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1556084.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2610..2942
                     /locus_tag="IXF98_001770"
     CDS             2610..2942
                     /locus_tag="IXF98_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1556085.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3561..3902
                     /locus_tag="IXF98_001771"
     CDS             3561..3902
                     /locus_tag="IXF98_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1556086.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3921..4211
                     /locus_tag="IXF98_001772"
     CDS             3921..4211
                     /locus_tag="IXF98_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556087.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            4403..4636
                     /locus_tag="IXF98_001773"
     CDS             4403..4636
                     /locus_tag="IXF98_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556088.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            5056..5727
                     /locus_tag="IXF98_001774"
     CDS             5056..5727
                     /locus_tag="IXF98_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1556089.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5780..7408
                     /locus_tag="IXF98_001775"
     CDS             5780..7408
                     /locus_tag="IXF98_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1556090.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAEKANIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAAKNAEGKYYSFCGR"
     gene            7917..8318
                     /gene="tnpA"
                     /locus_tag="IXF98_001776"
     CDS             7917..8318
                     /gene="tnpA"
                     /locus_tag="IXF98_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1556091.1"
                     /translation="MDNEYRYSKTTVSLVNYHFVFCPRYRRKIFVNSEVDARFKALVQ
                     EIAKENEWNIIAIETDKDHCHLLINVQPTYSPSKIMKIIKGKTSSIIRQEFEHLSKMP
                     SVWTRSYFVSTAGNVSSEIIKRYVENQRTRY"
     gene            8342..9460
                     /gene="tnpB"
                     /locus_tag="IXF98_001777"
     CDS             8342..9460
                     /gene="tnpB"
                     /locus_tag="IXF98_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1556092.1"
                     /translation="MKKAHKIRIYPNCRQEIQFQKTFGCVRFYWNFLLDQRITNYKKK
                     KENADYKEDKTTYASLKKMEEYAWLKEVEAQPLSQVAMDLNKAFKNTFNSTFGFPKFK
                     SKKYSKKSYRTAMGIKVNRNYFYVSKVGWVKMAETLRFNGKLMNVTISQSKTEKYFAT
                     FLVDIENFQKDPVTESIGLDLGLTHFCITSAGEKIGNERFYRSLEKRLVVEQRKLSRR
                     LEVAKKHNRKLEECKNYQKQRVKVARIHEKIFNQRNDFLHKLSSRITDENQIICIEDL
                     NVTGMVRNHKLAKSIADVSWSEFVRQLTYKCEWKGRTLVKIDRFYPSSQICSSCGHND
                     GKKELDIREWTCSNCGTNHDRDINASINIRTEGLSGIA"
     gene            9526..9891
                     /locus_tag="IXF98_001778"
                     /pseudo
     CDS             9526..9891
                     /locus_tag="IXF98_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            9904..10659
                     /locus_tag="IXF98_001779"
     CDS             9904..10659
                     /locus_tag="IXF98_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1556093.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQKQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSEKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFLLFLFIKKIRRRSE"
     gene            10656..11483
                     /locus_tag="IXF98_001780"
     CDS             10656..11483
                     /locus_tag="IXF98_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556094.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVAHQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETKTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIIL
                     LTPISDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            11493..11741
                     /locus_tag="IXF98_001781"
     CDS             11493..11741
                     /locus_tag="IXF98_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556095.1"
                     /translation="MKIIKMLAFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINMLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            11772..13883
                     /locus_tag="IXF98_001782"
     CDS             11772..13883
                     /locus_tag="IXF98_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1556096.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IRENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYSGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LDNAANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGIQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGTLTYDSNLRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLQSEKNIDNQAVAAVENVSNKTNVVTIGVDPNLPQVIVPRTGSTHLVTI
                     LVVSLVLLVLVTLSYVALKFN"
     gene            complement(13919..15970)
                     /locus_tag="IXF98_001783"
     CDS             complement(13919..15970)
                     /locus_tag="IXF98_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1556097.1"
                     /translation="MKLLNPKTVLAIGTFAATLGFAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNANKVLNDSKAT
                     QKQVDSAKNNLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDFTEESWNDFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPYMVTINHIDNQTGQVIATDQ
                     AQVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSQTITGNTTITFNYNQVHQINL
                     LCNNNYKNEGRVDILKEQIINVTHGESVTLTAPAIQGYVLDDRVEPTNSVTLNNVTES
                     QNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRNDD
                     PSISVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELVWLNNYRQQNGAAPM
                     QFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVDEI
                     LSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANPIF
                     K"
     gene            complement(16090..16299)
                     /locus_tag="IXF98_001784"
     CDS             complement(16090..16299)
                     /locus_tag="IXF98_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556098.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAIKLNKLAEETIDTNASTK"
BASE COUNT         5905 a         2480 c         3197 g         4954 t
ORIGIN      
        1 tatgacttgt acaaatttat ctgatattct cttattaaga actacctaat ttgggtagtt
       61 cttatttttt acattttaag tagtatttaa ttgcaatttg gtagtatttt tttattatct
      121 tattcctaga acggtagtac ttaatataaa tataggtagt atctaattta agtattaatg
      181 gtagtatcta atttaacagg tagtatgtaa ctactttaaa gtaatttttt tcagattaac
      241 acagtagtat cttattaaat aagttaattc taaaggtagt acataattat tatatatatc
      301 taacattggt agtacttatt gtatttttta gtagtatata atctattgtt ttctagccta
      361 aagccttgtc ttataagaat aatataatat atactactga aataaggtag tatatattac
      421 tagtagtggt agtatgtttt atatgcataa ggtagttctt aatttatttt ttgttttgat
      481 actggtagta cttaattatc cttaatttta aaaccaaatt ttattcatac gttcttctgg
      541 tagtatttaa ttataaataa tatttattta aagtagttct taatgacttt aaaggtagta
      601 cataattcat tttatttctt attttaggta gtatttaatt aaaaaacagt tttaatgcgt
      661 ttgcattttt tatttatctt tgctattttc gaaaagagta ggtaatttta cataaataaa
      721 acaacgtgaa agtagaggat ctacaatgac tgattttaat ttttttaagg cggatagagt
      781 ttacaacgaa ttattttatc aatttccaaa agtctttatt gtttctgacg aatacaaaaa
      841 aatgaaagat tcaactaaga ttgcctatat gcttttaaaa gcaagattag agatcgcaat
      901 cagcaaacgg caaatcgatg aagaaggtaa tgtttatttt acttatacga caaatgaact
      961 atgtagcgta ttaaactgcc aaaaacaaaa agcgatagca atcaaaaaag agttggaatc
     1021 ctttggttta ttattacaaa agcagatggg atttaacaaa cagttaggga aaaataatcc
     1081 taatagacta tatctagcag aattaaaagt ctcagaaaat gatatctact tactcgaaaa
     1141 atttgataga gagaataggg aaaatgttga taaatcagag ggtatgaaaa tcatacccac
     1201 cctcgacgaa aaatcagacg ctgaatccct tggggctcaa gagggtatga aaatcatacc
     1261 gtgccaaaac gttgataaat cagagggtat gaaaatcata ccagaactta ataataatat
     1321 attagacact aatagacaca atatagacac tgaaaaagac cgcctacaag atcaattgtt
     1381 gttagacaat tttgagacaa ttatgacaaa cgacagcatt gctacgtttg tccctgaacg
     1441 agtattaaat ttgataaaaa cattttcttc aagttacagt gaagctcaaa aaaccgtcca
     1501 gactattcat aatgcaaaga aaaaagctga aatagaaagt ggtatttcga tagtttttga
     1561 agaactcgat agttattatg tcaatgcaga acaagaatta tacacgacac tgttaaaagc
     1621 ctatcaaaaa ttaaaaaccg aaaaagtcga aaatatccag aacctgattt ttgtctatgt
     1681 aaaaaattgg tttatcgaaa aaccaatagc tgctaaagta tcaagtgaaa aacgtttgaa
     1741 ttatgaaagc tccccaagca ctattacgaa agactggtta gagtgaaact ttactgacaa
     1801 agtgattgtt gcttcatgga gtaacattaa aattaaaaat tttttattat tctaattata
     1861 ttacgaatat tacaaaataa tagctatttt aatagaattt ataagttaaa atattatgat
     1921 aaaaatcaca aacgtagcct tttacgtcat aagtgttaaa agaaataatt aatttcaaat
     1981 atagaaaagg gaacgtatgt ttgcatactc cttttttttg agttataata atatgcgaag
     2041 agagataatt tcattattta aacaagctaa aaatcttgat attacttctt ttcaggcaat
     2101 acgaatatta ttcgcttaca ttttttgtat aaatttaatt gtctgctatg cttgtgatga
     2161 attgattttc gttctgcact tttggatgat aaatttggac aattgaaaat acaaaaaaaa
     2221 gaaaggcggt atgaactcgg aaggctggta accttcgtca gcgttcatac acggcattaa
     2281 gatttggccc ttaatgcgtt aacctttcag tgttacggta ttacccgcaa tggatttagt
     2341 ataacagata ttcaaaagtg ataccacttt ttttggctgt gttaaggtta aaaatacgtg
     2401 atattacgat gaatctatgc ctaagcataa tgttataaaa atgtccgtgt ggctctttgc
     2461 cgtatgacga acgctgaaat tagagggaac gcatgtttgg atgcgttccc tcttttttgt
     2521 ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa cttgttaaaa
     2581 atatttaaag aaattgggtg gtttatttta tgtcatatac aggaattttg agtctcaaag
     2641 atatttgtca ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag aaactatcaa
     2701 ctggccaaag taaaaccgtt gtgcagtgta agaaatatat aatccagaaa gacaaagtct
     2761 cagaggaaat gatttattac attgggaaac aaaagcaaat aattttaaaa gatcctatcc
     2821 ccttaaaaga gctatatcca acaatcaaac atgtttatga ccaaaacggt gtactgattg
     2881 gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt ttaatcagct
     2941 gaattgatca ttctcataac ttgtatttta tcgtttttcg cttgcttgaa aatattgtcg
     3001 agacttctaa aagaacttag aatcattaga aaaataagga gtagacttga tctatcaaca
     3061 attcccttcg gggagtgacg gggtaggttt taacgattgg taacgtaaaa aattggtcaa
     3121 tcgagtaaat gcgcttgttt ggatagtggt gaaatgaatt tgtgtaaata ataagcgcag
     3181 agtaaacaag agggaattta gaagttagac aagttagaat agcgtctaga ttaatcatga
     3241 gcagctacta agggatctag gctttgacgc aaactcatgg aataaggagt aggtcttctc
     3301 gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta gatacttgcg
     3361 aggacgttgg tcattggatc aatcgagcat ttaccgctgt ctactagggt ttctcgtttc
     3421 ggacgccata aacgagtcga tattttttat gtatttttaa gtaaacgaaa aacgataaaa
     3481 tatgaacgag tcctgaataa agtgaaaaaa ttgatataaa acatagagaa aatgaaattt
     3541 acgtaatgga ggaatgacaa atgaaaaatt atgatccgaa tatacgattg ggcacacata
     3601 ctattaaggt ttctttccaa cgttgggatt acaaaggttt tgtgacgttt agaagaggcg
     3661 gaaattgtaa aggattagat gttttagcat taaacgagga tgatttatac gatcaaacat
     3721 tgactgataa tccaattgga ttcggcttgc tacctgaaga tgatgaaggt aatgaatggt
     3781 ttaaaatgac gcttatgaat gataatggag acgaattatc tgttgaagat acttggtcat
     3841 atttgagtga ttatattgtc agcgttgaaa tcattgaatt tgtagcggat aaagaagaat
     3901 aaaaactgga ggaataaatt atgaaatttt tcgaagtaaa atcaccatac tattatgcgt
     3961 taatcgcagc aaagaatgaa gaagaatgtg cagaattgta tgaacaagta gtaacagata
     4021 tcgaaaatcg tgaagaattt attcgatgtt tgcatgagtt aaatcgaaat gaagcaattc
     4081 aagaggatgc gaaaaaaatt agtgaagaaa cgttacaacc aatcggtatg gaggaagcta
     4141 caaaagacat ttttacaatt attgatgaac aaaaatcttg tgtattaagc attgattcta
     4201 cattggttta atagatccaa gattctataa tttttttcaa agtaaaaaga aattaggtaa
     4261 tgattctcag accaattact taggaaaata accatacaaa ttagatttta cagataaagg
     4321 attagaagga atcacggcga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
     4381 atttatttag gaggaaaatt agatggcgcg accagtttta gatgcgatgg atatggatac
     4441 tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta aagatgtatt
     4501 caaagcccta tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag aattcgacta
     4561 ttggagagag cataacggaa taaaagattt aaaaccagca cgtccagtta ttagacgaaa
     4621 aaaattgaag aagtaagacc aaagcgtatg gcgtacatag gcttttttat tttgataaaa
     4681 ataatgaaag gaaggaaaac tcatgaattg taaagaaatc gaaaatagaa aaaaagtgtc
     4741 gaaagaaatg gaagaaaagt tactaaaaac aatgaaacaa aaacaatgaa acaaaaacaa
     4801 tgaaacaaaa acaccttaaa agattatctg tcgcgcaata tatcaatgat atgcagttaa
     4861 caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa ctttgaacag ttgagacgaa
     4921 cttatgtgaa aaaaagttct aattgtcaac tattgttaga agtctcatga cattcattaa
     4981 tagaattcat ttttaaaact ttttctatgg aaatgagaaa aagttttttt gttttgtctg
     5041 aaaggaggca gatggatgaa aaaatggtta tttggttttt taggagtcgc tttaatcgtc
     5101 ttttgcagtg tgtttggcta tgttagctac caaaaacatg aaggagatgt gtttaagcaa
     5161 aatatagaaa aaaagatgcc cgtagatcaa atcaatgcac acgcaaagag ttataaggag
     5221 gatgcaacga acgtcaataa tgatatgtct ttggggcaaa tgttgtccat acaaaaagaa
     5281 gcaatcgaaa tgggcgtcaa taaacaagta tttgcacaaa ttcagattcc tgcacttggt
     5341 ttagcattgc cgatttttaa aggggcaaat caatacactt tgagtcttgg agcggctact
     5401 tatttttatg aggatgccga aatgggcaaa ggaaactatg ttctagcagg acacaacatg
     5461 gaaatgccag gtgttttatt ttcagatatt caaaagttgt cattagggga ggtgatggac
     5521 ttagttagta acgatggcgt ctatcgttac aaggtaacgt gtaaatttat cgtatctgag
     5581 tactttaaat taattgatgg cgttcctgaa gagaactctt tcttgtcttt accaaagaaa
     5641 ggagagaaac ccttattaac gttgtttact tgtgtttata cgtcacaagg aaaagaaaga
     5701 tacgtggtac agggagagct acaataacaa ggttgtaatc aacgcgagtc gttgaaatat
     5761 gaaatgagga gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg gaatgcttgt
     5821 tggaatgtta ggaatcattg caggctgctt tcagggatat cgagcatatg cagaagatgt
     5881 cacgcaaaaa acgccaccag agaaggtcaa tatcacggtt cataaattaa tgtacgatca
     5941 aggaacgcaa ttgaatgttg atattgacgg gatcaaaaat gatggttata cgcacgatca
     6001 atatcctaca ggtgtaacga aatacaacaa ggcagattat ggtgatgttg agtttacgtt
     6061 aggaaatatt acggatcaag tattaccgag ggaagattct gatctaacca acgcgaaagt
     6121 ggacgaaatc gttaaagatg tagaagacaa gggatctaac agtgagtacg ttaagaatgc
     6181 aaccaataaa attacttcag ctgtagatga aaatggtgag attacttttg ctgatcaacc
     6241 agcctatgtc aattccaaag ggaatgtata tgtggtttat gaaagtaaat ccgctgctgg
     6301 attagtcaca caaaaagcaa aaccaatggt tgtgattgcg ccaatgactg ataatacagg
     6361 ttcaggtttt ttaaaagaca ttcacttata tcctaaaaat attgtttcta agttgagttt
     6421 tgaattaacg aaatttggtg acgatggcac agctcaaagc aaacaaacac ctttaaaagg
     6481 ggcaaaattt gagttataca agggcgaacc tggaaaagga acaaaattag gagatcttgt
     6541 atcggatgat caaggaaagc tgactgctac tgatttgacg ttaggaaaat actattttgt
     6601 ggaagttcct tcagaagttg tagtgggatc agataaagaa ccaacagcag atcaatattt
     6661 attaggcgca gacgctcgca acgatgcgca caataaatta acctttgaaa ttacgaatga
     6721 tggcgtgaca agtgacttaa aagcttcata cgtaaactat aaagcaccag tgatcgataa
     6781 aacagtaaca aatggaacag gacaagaaca ttctttccaa attggtgacg cagtaaatta
     6841 tcaaggaacg attcatatcc caacagacat tgcaggcgga gcagacggta tcacagtaaa
     6901 cggagtgaaa agcgaaactt ctccttacag tgtatttaaa tggggggata cagcaggaca
     6961 aggactttct tatgtcgctg aaaaagcaaa tatcaaagtc acaaataatg atggttcagt
     7021 cgtattaaaa gaaaatactg actataaaat cgaaaattct gatagtggtt ttgtgatcga
     7081 ttttatcgta aataatggac aagtaagtga tacagttgcg aatttacatg gtcaagatct
     7141 aaaaatgact tataacatgt ttgtgaatga tagtgctgaa atcgcaaatc cattaacaaa
     7201 tagcgtggat ttcgtctata gcaacaatcc attcaatcag gaagaacacc atgaaaaaac
     7261 gaaagccgac gctgtaactt atggcgcaaa attcttaaaa gctgatggcg gactatttgg
     7321 aacaggaatc aaagcaacac cgcttgaagg cgcagaattt gctgctaaaa acgcagaagg
     7381 aaaatattat agcttttgcg ggcgataaaa cccacggttt taaccgtgtg gatgatagcc
     7441 cgcactgtac tgtaagagca tagggaaact tgtgcttagt ggaatatata aaatctcgta
     7501 tatcccaaca aagaaactga attgcgggga acccctaaag cgagtaaaac gacaacgtag
     7561 tcttgaaata ggcaaacgtg accgtggcga aagcagaaaa aatttactcg atagcatatg
     7621 gttaaatcct aagtgttgcg acaatgggca atctgcagcg aagactcgaa cagagtaacg
     7681 ttcaacgact attcctcttg agggaagtac acacaagcgt gtggaagtgg tttcgccgta
     7741 ctgggtaaag ccaacggata agatatagtc tgtgctggca cgaaagtgtc agaagttcat
     7801 cagagaactg cataggaagt agcgaaccta tgtgaacaaa ctccacataa aaaattcttc
     7861 attcttttgt gtatattaac attgatttag tacatggctt aacctaaaat taaggtatgg
     7921 ataacgaata cagatactca aagacaactg tatcgttagt caattaccac tttgtttttt
     7981 gcccaaggta tcgtcggaaa atattcgtta attctgaagt agatgctaga tttaaagcac
     8041 tcgttcaaga aatagcaaag gaaaacgagt ggaacatcat tgcaatagaa acagataaag
     8101 atcattgtca tttattaata aatgttcagc ctacgtattc tccttctaaa atcatgaaga
     8161 taataaaagg taagacatct agcataatcc ggcaagagtt tgaacactta agtaaaatgc
     8221 caagtgtttg gacacgttca tactttgttt caaccgcagg aaatgtatct agcgaaatta
     8281 tcaagcgata tgttgagaac caaagaacaa gatattgatt gcgttaagga ggtgtgcaga
     8341 aatgaaaaag gctcataaaa tcaggattta ccccaactgt cggcaagaaa ttcaatttca
     8401 aaaaacattc ggatgcgttc gtttctattg gaactttcta ctagatcaac gaataacgaa
     8461 ttacaagaag aaaaaagaaa atgccgatta caaagaagat aagacaacgt atgcttcatt
     8521 gaaaaaaatg gaagaatacg cttggctaaa agaagttgaa gctcaaccac ttagccaagt
     8581 agccatggat ctaaataaag catttaaaaa tacgttcaac tctacttttg gatttcctaa
     8641 attcaaaagt aagaagtatt ccaaaaagtc ctatcgaaca gctatgggta taaaggtgaa
     8701 tagaaactac ttttatgtat ccaaggttgg ctgggtaaaa atggctgaaa cgttgcgttt
     8761 caatggtaaa ttaatgaatg tgacaatcag ccagtcaaaa acagaaaaat attttgcaac
     8821 ttttttagtc gatattgaaa acttccaaaa agatcctgtg acggaatcca ttggtttaga
     8881 tttgggtctt actcatttct gcataacttc cgcaggtgag aagataggaa acgagagatt
     8941 ctatcgatca ttagaaaaac gcttagtggt agagcaacgt aaactttcaa gacgcttaga
     9001 agtagcaaag aagcataatc gtaaattaga agagtgcaaa aactaccaaa agcaaagagt
     9061 gaaagtggca cgtatccatg aaaagatttt taaccaacga aatgactttt tacacaaact
     9121 gtcttctcgt ataactgatg agaaccaaat catttgtatt gaggacttga atgtaacagg
     9181 catggttaga aatcataaat tagctaaatc aatagcagac gtatcttgga gtgaatttgt
     9241 aaggcaatta acatacaagt gtgaatggaa aggtcgaacg ttagtaaaaa tcgaccgttt
     9301 ttaccctagc tcgcaaatat gttcttcttg tggtcataat gacggtaaga aagagttaga
     9361 tattagagaa tggacttgta gcaattgcgg aacaaatcat gacagagata tcaacgctag
     9421 tataaacatt agaaccgaag gactttcggg gatagcttag taaatttgcg cccgtttggg
     9481 tgtattacct aagaacccca tgactttagt cgtgtggagt gtcagggtgg attagttgat
     9541 acggataaag acggtgtcaa agaagcagta tgggtagaca atgtagcaaa tgcagcgatc
     9601 ctaaaatcag ataaagaagg gcgctttgaa atcactggtt taactgaagg tgaatattca
     9661 ttggaagaaa caaaagcacc aactggttac caaaaattaa cagaagatat tgcatttaaa
     9721 gtaaacaaaa actcatttaa agaggaaaat cgaatcactg ttaaaaataa tcaaaaagca
     9781 gctattccat taacaggtag taatggtttc caaacgtatg tattgatctc ttgcttgttg
     9841 ctaggagcag gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag
     9901 ctaatgatta ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt
     9961 tggttgctgt cggcagtagc agtctattta attccagttg tggaaacaaa tgtagctaaa
    10021 tttcaacaaa agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg
    10081 gatgttgcga aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct
    10141 ttaaaactac caatttatga cggaacttcc gaaaaaattt tagagaatgg tgtagggatt
    10201 acggaaggaa ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcagggcac
    10261 agtggacttt ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa
    10321 ttctttatta aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt
    10381 aaagcagaag aattacaaaa gaattattta gattatctca tgcctgaaaa agaccaagat
    10441 aggattacgt taatgacctg cacgccaaaa ggtgtcaact cacaccgctt tttagtgtat
    10501 ggtgaacgag tttcattcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg
    10561 aaatcaattt cattggattg gcgattagca ctgctattcg ctttttttgc gttcctatta
    10621 tttttattta taaaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg
    10681 tttgtgtagc agctctcttt gcaactgcag cttactcaca gatatctttt gctgatagca
    10741 gtaccactcc aactaccagt cagacaacag atagttcaac aactgcaaca agcgacacgg
    10801 acagttcgaa gaacactgca gaagaaacgg cagattcaag tacgagttct actgaatcag
    10861 cggaatcaac gtcagaaagt tcttcagaga cttcagaaaa actgcaagaa gtagaagata
    10921 cgcaagtaat caagcaaaac attcatattc aaaaaattat ttcttcgaat cgaatcaaca
    10981 atcaaggaag gcaagtagct catcaagaag gtgtcaatgg cgcaaagttt attgtctacg
    11041 atatcacgaa tatcttagaa gaaatgacac aagatgataa tgagaaaaat caatcggtag
    11101 aaataattga gtcaaaattg aaagatcgag caaaaaaact ttcatacgac cagttgaaaa
    11161 aagtgactga aggagaaacc aaaacaattg atgatcagga aggtgtgatt gatttttcaa
    11221 ttgaagtacc tgcaaacaaa aaacaagcat actatatcgt caatgaatca agccctgaaa
    11281 acatttcaaa tagcgaagat attattttat tgacacctat ttcagatgaa aagggtgaat
    11341 ttttgacgga tgtttggtta tatcctaaat caaagaaatc agagccgaaa gaagaaatta
    11401 aaaaagttgt atcaactggt gtaaagaaaa acttttttga taattgttgg gattttttca
    11461 ctcatttatt ttttaacgat taggatgaaa tgatgaaaat aataaaaatg ttagctttta
    11521 atctatcttt tttagccgca acaatattgt gttgcggttt agttttttct caattatcaa
    11581 tttatccaag atatcaacga ttgttcgttc attttctaaa tatccagcat gcttccatgc
    11641 cactagaagt gattaacatg ctcttaatca atccaatttt tatggctagt ttgtttcttc
    11701 ttgcaggatt ggtaacgtgg gtattaggaa aaaaagaatg agaagcttac aatcgtttaa
    11761 caaaataata gatggagggc attatggaaa ataaaattca ttgcactacg ggaagagtat
    11821 ttactttagt gttgtttttg ttccttcttc tttttgcaag gggaactgca gtccaggcct
    11881 tagaaaaagt tgatccgagt caaatcagag aaaataataa tctggtactt tatcatgata
    11941 gtgattcatt gagaaatatc gaagtaactg ggatcaatgg atttaagata ctagatgagt
    12001 attcaactaa aaaacaaaaa gtacttgctt ggccaaaagt caaagatttg aaaagcacgt
    12061 caaaaattgt tgcccattat caaaaggtgg gcacttatct aggaaaagaa attgattgcg
    12121 acgtaacttt ttctaatttt gtagcgcacg ctggggttaa ccaagacgga ttagctaaaa
    12181 caattaatgg gaaaaagtat tataattatt tgctctttga taaaaacatt tatcgaggct
    12241 ttttcatgca gtcaatcaac gttatgaatg taaaactaga ttttaagtat tcaggatcaa
    12301 aaacaaaagt agctttgagt gaaccacagc cagaaattaa ttcaaaaatg agatcgatga
    12361 gagcagctgg tattgagttg aatgatacat ttctatctat ttacagttta aatggacagt
    12421 atcagcaacg tgtgtcttgg tataatgaca ataatgaatc tgtcggtgaa cgtaatattg
    12481 atatgagaga atttgttggg tatgaaaaaa tgggtagctt accttatttt gtcacaacag
    12541 atacagcatg tgcagaatac aaagctcctg cgttatcaac aaacaattta acttcaaaag
    12601 tagtgggagg acgtgcagaa aaggcttata gctcgaatga tcatttcacc gatgttgtag
    12661 gagctgatac ttatcacaga agtggtgtaa cgtatacgct tcaaggcgct tccccaacat
    12721 tcatgattgg cgcaaatacg aatagtatga tgtttagctt tgatactgca ttgctatgga
    12781 caccacaacc atcgaagcct acaaaagaag tgtttaacaa agctaatact gaagaggcag
    12841 cacacaatat tgacaaaaaa gtgattccac aaggatcaga tgtttactat catattcacc
    12901 aaaagtttga tgcattaaca gtcaacacaa tgaacaaata caaatcattt aaaatcactg
    12961 atacctttga cagcaaaaat tttgatatgg tatcggatgg gaaaaactat gatggcgcat
    13021 tgtggtatgt tgaagcgaat gggacttgga aaaaagctga tacaggagga aaattaactg
    13081 tatcaggtaa tacagtcact tatactgctt ctgacagctt tttagataac gctgcaaatt
    13141 acggtaggga ttatattttg cagctccata tgaaagcaaa aaatgatgca aaaggaattg
    13201 caccaaatgt ggctactgta acgtttaaca atgatccgaa caaaggaata caaaaaacaa
    13261 atacagtaga aaactatttc tctcttaaac caaataaaaa agtggaacaa gatgggaaag
    13321 atgttgatgg tcgaaatgat ggtaaaaaag gaacgcctac agctccttta aatgcaggat
    13381 ctgaagtaca atatctagtc acacaaaaat ggcatacaaa aggtgtagac attgcgtcag
    13441 atcattacaa gcaatttagt attcaagatc cagtggaagc tcgtttgacg tataaagaag
    13501 gttctgcgcg agtgatagat atgagtacag gaaaagatat tacttcagaa ggtacactaa
    13561 cctatgatag caatttaaga acgctgaaat gggaagcttc cgctgatttc ttaagtaaaa
    13621 atcttttaga tggacgagaa attcaactga tatttacagc taaaactcca ctacagtcag
    13681 aaaaaaatat tgataaccaa gccgtagcag cagtagagaa tgtttctaat aaaacgaatg
    13741 ttgtaacaat tggtgttgat cctaacttgc cacaagtcat tgttcctaga acaggttcta
    13801 cacatttagt aacaatttta gtggttagct tagtattact tgtgttagtt actcttagtt
    13861 atgtggctct gaagttcaat taaacaaaaa aacaaggctg aaaatcagcc ttgtttttct
    13921 atttgaaaat tggattggca ctaataaata cgttgtctga agaaagtgat ccatctgcag
    13981 aaacttcata agcatgagat aacccaacga tgctttgatt gttgtaaagt aagtttgcgt
    14041 tatgagcagc gctatctttc cacataatca tggcattcac tgctcccgta tctgaaagga
    14101 tctcgtcaac gtaaaggtag aaactattga tattttctcc tttagcgccg tagcagccta
    14161 aactttctaa gagatcttgg aaagtaccgc cgccaggtcg ataatgactg aagcttactt
    14221 gtaattcttt tgcacgtata tcagcagctt gttgaacgat atcattaaat tgcattggag
    14281 ctgcaccgtt ttgttgacga taattattta accatacaag ttctttttga cgcacatagt
    14341 tgtttaaatc acttaatgag atatttttgt atttgaacgt gatcgttcga ttttggtcaa
    14401 cattattgat tactcgttgg ccgtttttat ctacgctaat ggaaggatcg tcatttctac
    14461 acaagaaata attttgatca gtcatatttt tccaaggagt agagaagcta tccccttcgt
    14521 atactgtttt tgattcacta gagagaacag cgttggtatc agcatttaca tgattaacag
    14581 tcacattaaa tttacgagtg tagttgaaaa tataattttg actttcagtc acattgttta
    14641 aggttactga atttgttggt tctacccgat cgtctaaaac atatccttga atagcaggag
    14701 cagttaaagt gacactttca ccgtgagtga cgttgattat ctgttctttc aaaatatcaa
    14761 ctcgcccctc atttttgtag ttgttattgc acaacaaatt gatttgatgc acttgattat
    14821 aattgaaggt gattgttgta ttacctgtga tcgtttggct ttgagtatca ttaccaatca
    14881 ctttgtagct aaaattatct ttagcgcctt cgctatattt aaatgctttt gccttagcgg
    14941 tgtaagtaga accagaagtc acttgtgctt gatctgtagc aatcacttga cctgtttgat
    15001 tgtcaatatg gttaatggta accatatatg gcgtttcagg tttgtgttgc aaccctttaa
    15061 atgcagcatc tagactattt ttagcattat ttacttgggc ttgagaagcc ttagaatcgt
    15121 ctagtacttt tttagcattg gataaagcag tctgaaaatc attccaagat tcttctgtaa
    15181 aatctccttt agcagttgat tttactcggt tgtataaagc ttgtaattca gctttattca
    15241 cagtaggtgc tggttttttc tttaatccct tgtaagcaga atctaaatta tttttagcac
    15301 tatccacttg tttttgagtc gcttttgagt catttaacac tttattagca ttatttaaag
    15361 cagtttgaaa tgcgttccag gtattgtcgg tatagtcacc ttttttcgtt cccttaacgc
    15421 tcgtataaag agcttctaaa gctgatttat tcactttggg tttagaggtg tcagcgccat
    15481 accaaccgat accttcgtat ttccagccat agtctttggt taaccattgt acttctttcc
    15541 aacttgcagt gtagtgatgt gagccagatt ttacaccagg aaggaaaagg cggtagatcg
    15601 gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccag ccgtaatttt
    15661 tagtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga agacctttat
    15721 tgtataaacg atagacaaga tgaccaccgc tagtcggtgc ttcccaagcg gggccttcat
    15781 aattccccca cttaatcttc actaggtgat cacgttcttt tgtgctagag gtgtaaaaat
    15841 gttcatggtt atttggatta taaaggcgat acatgacgtt agttgaagca gcatcagcag
    15901 atccagcgaa accaagtgtg gcagcaaatg ttccaatagc taaaacagtt tttgggttta
    15961 ataatttcat attttcaact cctaaaaatt agttaaaatc gattacaaaa tcattatata
    16021 tcggtaaacc gatatggtca ataaaaaaat taagaaaagc ctcatgtatt tctggctttt
    16081 cttaatttgt tacttagttg aagcgttagt atcaatggtt tcttcagcta atttatttaa
    16141 ttttatagca ttgatgaatc tcatattgtc tatactagag cgcttatttg cgagatcact
    16201 gaccgtagtt aaaggtacgc cagattctat agatatagaa cgaactgatt taccactatc
    16261 aaacagcaac cattcgattt tctttttatc agcgtacatt gcaatgctcc ctcttgattt
    16321 tttctttatt atagctcttt tttgtaataa atcacaatat caagagagaa agtttttata
    16381 gaaaggtggg aaattatgag tatccgttcc ccgtgtatca aaatgaggtt tgttgctact
    16441 ttcgttattt gatatactaa aataaaacaa cgaaagaaga aataaaatga agaaaaagca
    16501 actatggttt gtctctatgt ttgcaatatt ttttat
//
LOCUS       DACETN010000060        15887 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DACETN010000060 DACETN010000000
VERSION     DACETN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.062"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(58..360)
                     /locus_tag="IXF98_001785"
     CDS             complement(58..360)
                     /locus_tag="IXF98_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556099.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IXF98_001786"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IXF98_001786"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="IXF98_001787"
     CDS             complement(1117..1890)
                     /locus_tag="IXF98_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1556100.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="IXF98_001788"
     CDS             2034..2384
                     /locus_tag="IXF98_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1556101.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="IXF98_001789"
     CDS             2365..3081
                     /locus_tag="IXF98_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1556102.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="IXF98_001790"
     CDS             3081..4529
                     /locus_tag="IXF98_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1556103.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="IXF98_001791"
     CDS             complement(4599..5108)
                     /locus_tag="IXF98_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556104.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="IXF98_001792"
     CDS             complement(5098..5823)
                     /locus_tag="IXF98_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556105.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6046..8166)
                     /locus_tag="IXF98_001793"
     CDS             complement(6046..8166)
                     /locus_tag="IXF98_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1556106.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8396..9574
                     /locus_tag="IXF98_001794"
     CDS             8396..9574
                     /locus_tag="IXF98_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1556107.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9590..10348
                     /gene="aroD"
                     /locus_tag="IXF98_001795"
     CDS             9590..10348
                     /gene="aroD"
                     /locus_tag="IXF98_001795"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1556108.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10371..10856
                     /gene="ybaK"
                     /locus_tag="IXF98_001796"
     CDS             10371..10856
                     /gene="ybaK"
                     /locus_tag="IXF98_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1556109.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10927..12180)
                     /locus_tag="IXF98_001797"
     CDS             complement(10927..12180)
                     /locus_tag="IXF98_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1556110.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12297..13646
                     /gene="gdhA"
                     /locus_tag="IXF98_001798"
     CDS             12297..13646
                     /gene="gdhA"
                     /locus_tag="IXF98_001798"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1556111.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13758..15104
                     /locus_tag="IXF98_001799"
     CDS             13758..15104
                     /locus_tag="IXF98_001799"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1556112.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15412..15798)
                     /locus_tag="IXF98_001800"
     CDS             complement(15412..15798)
                     /locus_tag="IXF98_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1556113.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5193 a         2843 c         3188 g         4663 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg
     6001 agcagcgaca ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact
     6061 tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg
     6121 tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt
     6181 taatttctct aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact
     6241 attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt
     6301 atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat
     6361 agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac
     6421 aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc
     6481 taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc
     6541 aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat
     6601 ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc
     6661 aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag
     6721 gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac
     6781 ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt
     6841 ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac
     6901 agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg
     6961 gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata
     7021 agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa
     7081 tgtaccagcg ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc
     7141 aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa
     7201 gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg
     7261 atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg
     7321 tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg
     7381 gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc
     7441 atttggtgct gcataatgtt cttttacata attttctact tctttcatat cattgggact
     7501 tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc
     7561 attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg
     7621 acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat
     7681 cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag
     7741 cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg
     7801 aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt
     7861 aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag
     7921 aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat
     7981 tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc
     8041 actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa
     8101 aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca
     8161 tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta
     8221 cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca
     8281 gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga
     8341 caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa
     8401 agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa
     8461 agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca
     8521 aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt
     8581 gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa
     8641 agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg
     8701 agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc
     8761 ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat
     8821 ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga
     8881 gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt
     8941 aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga
     9001 agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag
     9061 ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt
     9121 cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga
     9181 tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag
     9241 aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa
     9301 aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt
     9361 agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt
     9421 ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt
     9481 atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa
     9541 agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt
     9601 aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt
     9661 ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga
     9721 atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt
     9781 tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga
     9841 aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca
     9901 cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag
     9961 actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa
    10021 agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc
    10081 cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga
    10141 gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa
    10201 tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc
    10261 cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat
    10321 tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga
    10381 aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag
    10441 agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc
    10501 cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc
    10561 ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag
    10621 ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac
    10681 gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg
    10741 cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag
    10801 cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca
    10861 tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt
    10921 tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc
    10981 tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag
    11041 accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc
    11101 gccagttaca aatgatttct caataggaaa agttttgttg attgatagct taaactcttt
    11161 gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc
    11221 catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc
    11281 attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg
    11341 tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa
    11401 ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt
    11461 gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa
    11521 agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga
    11581 tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat
    11641 cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat
    11701 aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt
    11761 gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg
    11821 attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg
    11881 atcctcttct tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt
    11941 ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc
    12001 atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt
    12061 tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat
    12121 cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat
    12181 tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg
    12241 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    12301 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    12361 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    12421 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    12481 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    12541 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    12601 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    12661 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    12721 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    12781 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    12841 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    12901 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    12961 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    13021 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    13081 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    13141 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    13201 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    13261 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    13321 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    13381 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    13441 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    13501 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    13561 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    13621 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    13681 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    13741 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    13801 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    13861 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    13921 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    13981 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    14041 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    14101 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    14161 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    14221 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    14281 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    14341 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    14401 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    14461 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    14521 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    14581 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    14641 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    14701 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    14761 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    14821 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    14881 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    14941 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    15001 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    15061 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    15121 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    15181 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
    15241 cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga
    15301 ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca
    15361 gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag
    15421 gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat
    15481 gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt
    15541 ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc
    15601 ataagcatct acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat
    15661 atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt
    15721 agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa
    15781 ccctaaaatt tttttcatga aagatctcct tttttatttt tattataccc gtggtgctag
    15841 ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtc
//
LOCUS       DACETN010000061        15860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DACETN010000061 DACETN010000000
VERSION     DACETN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.063"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(12..482)
                     /locus_tag="IXF98_001801"
     CDS             complement(12..482)
                     /locus_tag="IXF98_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1556114.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IXF98_001802"
     CDS             complement(641..1099)
                     /locus_tag="IXF98_001802"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1556115.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IXF98_001803"
     CDS             complement(1270..2415)
                     /locus_tag="IXF98_001803"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1556116.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IXF98_001804"
     CDS             complement(2428..3345)
                     /locus_tag="IXF98_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1556117.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IXF98_001805"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IXF98_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1556118.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IXF98_001806"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IXF98_001806"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1556119.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IXF98_001807"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IXF98_001807"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1556120.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IXF98_001808"
     CDS             6871..7752
                     /locus_tag="IXF98_001808"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1556121.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IXF98_001809"
     CDS             7749..8465
                     /locus_tag="IXF98_001809"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1556122.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IXF98_001810"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IXF98_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1556123.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IXF98_001811"
     CDS             9182..10183
                     /locus_tag="IXF98_001811"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1556124.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IXF98_001812"
     CDS             10180..11541
                     /locus_tag="IXF98_001812"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1556125.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IXF98_001813"
     CDS             11501..12652
                     /locus_tag="IXF98_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1556126.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IXF98_001814"
     CDS             12627..13796
                     /locus_tag="IXF98_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1556127.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IXF98_001815"
     CDS             13971..15350
                     /locus_tag="IXF98_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1556128.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="IXF98_001816"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="IXF98_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5186 a         2798 c         3378 g         4498 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatttg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DACETN010000062        15825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DACETN010000062 DACETN010000000
VERSION     DACETN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.064"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(266..466)
                     /locus_tag="IXF98_001817"
     CDS             complement(266..466)
                     /locus_tag="IXF98_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1556129.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IXF98_001818"
     CDS             complement(771..2003)
                     /locus_tag="IXF98_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1556130.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2260..2829
                     /locus_tag="IXF98_001819"
     CDS             2260..2829
                     /locus_tag="IXF98_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1556131.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3007..3447)
                     /locus_tag="IXF98_001820"
     CDS             complement(3007..3447)
                     /locus_tag="IXF98_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1556132.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3605..4369
                     /gene="map"
                     /locus_tag="IXF98_001821"
     CDS             3605..4369
                     /gene="map"
                     /locus_tag="IXF98_001821"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1556133.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4401..5324
                     /locus_tag="IXF98_001822"
     CDS             4401..5324
                     /locus_tag="IXF98_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1556134.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5400..6539
                     /locus_tag="IXF98_001823"
     CDS             5400..6539
                     /locus_tag="IXF98_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1556135.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFTAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6532..7332
                     /locus_tag="IXF98_001824"
     CDS             6532..7332
                     /locus_tag="IXF98_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1556136.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7332..8159
                     /locus_tag="IXF98_001825"
     CDS             7332..8159
                     /locus_tag="IXF98_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1556137.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8137..8871
                     /locus_tag="IXF98_001826"
     CDS             8137..8871
                     /locus_tag="IXF98_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1556138.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8971..9837
                     /gene="rfbA"
                     /locus_tag="IXF98_001827"
     CDS             8971..9837
                     /gene="rfbA"
                     /locus_tag="IXF98_001827"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1556139.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9851..10423
                     /gene="rfbC"
                     /locus_tag="IXF98_001828"
     CDS             9851..10423
                     /gene="rfbC"
                     /locus_tag="IXF98_001828"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1556140.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10445..11473
                     /gene="rfbB"
                     /locus_tag="IXF98_001829"
     CDS             10445..11473
                     /gene="rfbB"
                     /locus_tag="IXF98_001829"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1556141.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11571..12422
                     /gene="rfbD"
                     /locus_tag="IXF98_001830"
     CDS             11571..12422
                     /gene="rfbD"
                     /locus_tag="IXF98_001830"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1556142.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12456..14489
                     /locus_tag="IXF98_001831"
     CDS             12456..14489
                     /locus_tag="IXF98_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556143.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14533..15813
                     /locus_tag="IXF98_001832"
     CDS             14533..15813
                     /locus_tag="IXF98_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1556144.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4861 a         2732 c         3281 g         4951 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aatgactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg cagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccag ccgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg
     2101 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
     2161 gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg
     2221 gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt
     2281 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     2341 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     2401 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     2461 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     2521 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     2581 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     2641 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     2701 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     2761 attgaaaaga tgatacaaga cagtatagat gctatcttga aattcaaaga accaatagaa
     2821 atcgattgaa tatactcaaa agttccacaa atcagtagat aacaagagat gtgtcaaaat
     2881 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     2941 gaatgcaatg aataaaagag ccgtacagat tgaataaatc tggatggctc ttttattgca
     3001 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     3061 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     3121 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     3181 tccgcagaca ccgtaaattt ttcccgatag atccaattct tgcaagtctt cataaaagtc
     3241 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     3301 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     3361 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     3421 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     3481 tatgaattat accaaaacat cattgaaaag aaaataaaac ctagagacac ctggggaatg
     3541 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     3601 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     3661 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     3721 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     3781 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     3841 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     3901 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     3961 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     4021 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     4081 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     4141 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     4201 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     4261 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     4321 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     4381 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     4441 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     4501 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     4561 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     4621 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     4681 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     4741 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     4801 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     4861 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     4921 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     4981 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     5041 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     5101 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     5161 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     5221 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     5281 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     5341 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     5401 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     5461 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     5521 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     5581 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     5641 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     5701 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     5761 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     5821 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     5881 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     5941 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     6001 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     6061 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     6121 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     6181 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     6241 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     6301 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     6361 cgatcatatt gattattgcc atgttgttta ctgcaatttt attatttgaa ttgattggtc
     6421 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     6481 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     6541 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     6601 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     6661 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     6721 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     6781 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     6841 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     6901 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     6961 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     7021 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     7081 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     7141 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     7201 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     7261 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     7321 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     7381 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     7441 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     7501 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     7561 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     7621 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     7681 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     7741 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     7801 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     7861 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     7921 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     7981 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     8041 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     8101 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     8161 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     8221 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     8281 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     8341 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     8401 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     8461 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     8521 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     8581 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     8641 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     8701 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     8761 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     8821 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     8881 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     8941 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     9001 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     9061 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     9121 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     9181 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     9241 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     9301 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     9361 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     9421 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     9481 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     9541 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     9601 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     9661 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     9721 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     9781 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     9841 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     9901 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     9961 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    10021 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    10081 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    10141 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    10201 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    10261 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    10321 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    10381 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    10441 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    10501 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    10561 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    10621 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    10681 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    10741 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    10801 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    10861 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    10921 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    10981 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    11041 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    11101 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    11161 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    11221 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
    11281 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
    11341 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    11401 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    11461 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
    11521 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
    11581 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
    11641 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
    11701 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
    11761 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
    11821 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
    11881 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    11941 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    12001 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    12061 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    12121 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    12181 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
    12241 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    12301 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
    12361 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    12421 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    12481 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
    12541 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    12601 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    12661 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
    12721 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
    12781 acggacaaaa catgatcatt gcgtataatt ctcctgcaaa ggatattacg gttatcggaa
    12841 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    12901 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    12961 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    13021 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
    13081 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    13141 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    13201 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    13261 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    13321 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    13381 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    13441 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    13501 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    13561 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    13621 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    13681 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    13741 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    13801 catggtctta tttctatgcg attacgcatt cgaaaataga taattatttt tcggattttg
    13861 aaaccatcgc agttgctgtg ttgggaacag tgattttagc atttgcatta tttggctgga
    13921 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
    13981 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    14041 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    14101 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    14161 aggaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    14221 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    14281 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    14341 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    14401 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    14461 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    14521 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    14581 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc agggttaggt
    14641 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    14701 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    14761 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    14821 ggagatttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    14881 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    14941 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    15001 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    15061 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
    15121 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
    15181 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    15241 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    15301 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    15361 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccaa
    15421 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    15481 ttgataggat ttgggattgc tactttgctg cttatccttt ttattggtcg cttaacgttg
    15541 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    15601 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    15661 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    15721 gaagaggcat atgacgatat gacttatgat gatacatctt atggggaaca accagatagc
    15781 gctcattctt ccaggatcca acgctatcat tgataggcac aaaat
//
LOCUS       DACETN010000063        15456 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DACETN010000063 DACETN010000000
VERSION     DACETN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.065"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            361..1524
                     /locus_tag="IXF98_001833"
     CDS             361..1524
                     /locus_tag="IXF98_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1556145.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            1616..2371
                     /locus_tag="IXF98_001834"
     CDS             1616..2371
                     /locus_tag="IXF98_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1556146.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2429..4468
                     /locus_tag="IXF98_001835"
     CDS             2429..4468
                     /locus_tag="IXF98_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1556147.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEQPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFANNGTLVY
                     PKLIADKETKDKKNVIGETALQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGKENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4577..5728)
                     /locus_tag="IXF98_001836"
     CDS             complement(4577..5728)
                     /locus_tag="IXF98_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1556148.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5882..6412)
                     /locus_tag="IXF98_001837"
     CDS             complement(5882..6412)
                     /locus_tag="IXF98_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556149.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6511..6861)
                     /locus_tag="IXF98_001838"
     CDS             complement(6511..6861)
                     /locus_tag="IXF98_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1556150.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7018..8547)
                     /gene="ahpF"
                     /locus_tag="IXF98_001839"
     CDS             complement(7018..8547)
                     /gene="ahpF"
                     /locus_tag="IXF98_001839"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1556151.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8557..9120)
                     /gene="ahpC"
                     /locus_tag="IXF98_001840"
     CDS             complement(8557..9120)
                     /gene="ahpC"
                     /locus_tag="IXF98_001840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1556152.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9264..9926)
                     /locus_tag="IXF98_001841"
     CDS             complement(9264..9926)
                     /locus_tag="IXF98_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1556153.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10110..11756
                     /locus_tag="IXF98_001842"
     CDS             10110..11756
                     /locus_tag="IXF98_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1556154.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11747..12829
                     /gene="uxuA"
                     /locus_tag="IXF98_001843"
     CDS             11747..12829
                     /gene="uxuA"
                     /locus_tag="IXF98_001843"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1556155.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12937..14490)
                     /locus_tag="IXF98_001844"
     CDS             complement(12937..14490)
                     /locus_tag="IXF98_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1556156.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            14638..>15456
                     /locus_tag="IXF98_001845"
     CDS             14638..>15456
                     /locus_tag="IXF98_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1556157.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPD"
BASE COUNT         5154 a         2830 c         2905 g         4567 t
ORIGIN      
        1 tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat
       61 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
      121 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgtcgg attaaggctt tttccaatag
      181 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
      241 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
      301 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
      361 gtgaaaaaaa gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg
      421 atcggtcttt tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat
      481 acaaaaagtc tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc
      541 ttagctcgtt cagtcaaact acactattta cttcacccta aaatagcagg atacggttta
      601 gccttatcga ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac
      661 ggcgcacaac gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat
      721 ctttttttga ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat
      781 ctaaaaaaac cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag
      841 attgctggag cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt
      901 ccatttaaaa aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca
      961 ggcggggtat tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat
     1021 gaaaggatcg cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg
     1081 acccattcgt tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt
     1141 atcacaaaaa aagggtatct tccagagaca gaaacagact ttattttttc gattattaca
     1201 gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg
     1261 agaatctttt gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga
     1321 ttcggaactt tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg
     1381 ccaatgactg gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt
     1441 tctttaggaa tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttgccg
     1501 ctctatcgac ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata
     1561 aagtacaatc ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa
     1621 agtatctcaa aaaatcatat tatctatttt gttactgctt acattagtag ttggatattt
     1681 ttcgattgag tttatccatg gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt
     1741 agagccgaaa agtgtcccta ccacactaaa tgtggctttg attggttcag atgctcgttc
     1801 gaaagacgaa aacggtcgct cggattcact tctggttgca caatacaacc agaaaacaca
     1861 acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag gttacggaat
     1921 gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc aaacattaaa
     1981 ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag attttatcga
     2041 ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat ctctagattt
     2101 agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt tactccagta
     2161 tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc agcagcaagt
     2221 gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc tacccaaagc
     2281 ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc ttctggattg
     2341 tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag tgaaataaag
     2401 gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg
     2461 aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt
     2521 ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca
     2581 atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa
     2641 ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata
     2701 cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga
     2761 ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga
     2821 tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc
     2881 caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc
     2941 agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa
     3001 acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa
     3061 agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag
     3121 ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact
     3181 tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc
     3241 tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata agaatccaga
     3301 attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct
     3361 tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt
     3421 tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc
     3481 acaaaaaaca gcttttgaca gtctaggagg aaaagctgga tcaactgttg cgacaacgcc
     3541 aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac
     3601 aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac agccattcat
     3661 cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat
     3721 cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg
     3781 gcaaaaagac agttcttggg gatcttatca agtaacgcgt gtcagtagtg atgtatcaca
     3841 agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt
     3901 gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct
     3961 tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga
     4021 tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattaatc ctatccagca
     4081 agcagcaatg tattctgttt ttgccaacaa tggcacactt gtctatccta aattgattgc
     4141 agataaagag acaaaagata agaagaatgt catcggcgaa acagcattac aaacgatcgt
     4201 gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt
     4261 agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa
     4321 ggggaaagag aacagtttct tgtttgcttt caatcctgat aaccaaggtt atatgatggt
     4381 cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt
     4441 gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt aaaaggactg
     4501 tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc tctttcacaa
     4561 cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg agaacaagtg
     4621 gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac tgtggttcga
     4681 tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca cgcgaaatca
     4741 taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat
     4801 aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata aagagtagtt
     4861 gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat actggtataa
     4921 atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc tttgtctgac
     4981 tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca agatccgcta
     5041 cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa tttgcgtaca
     5101 ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag aaaataaaaa
     5161 caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta gaagcttccc
     5221 ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca tctaccacag
     5281 aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct tttaatactt
     5341 ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc gcttcatttc
     5401 ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag atgatcccaa
     5461 gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa tcactttcca
     5521 gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg actagaatat
     5581 caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt aattgtccaa
     5641 tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata
     5701 tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa atatagcccg
     5761 aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa aaaaccaaca
     5821 atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata actaaaaatg
     5881 attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga
     5941 aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca
     6001 ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt
     6061 ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa
     6121 gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca
     6181 ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt
     6241 aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga
     6301 taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag
     6361 cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa
     6421 acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat
     6481 gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt
     6541 gccttttgct agctcatcga tcattttatc taaataacga atttctttca tcaagttatc
     6601 ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt
     6661 ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc
     6721 tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat
     6781 acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt
     6841 aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta tttactactt
     6901 atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa aggattgtga
     6961 caaaattctt gtcaccatcc ttttttccgc tctctgactt tttacaatct tcatagctca
     7021 agtgcgcatc agataatcga atgctcctaa agctgcagta gctcctgaac ccattgaaac
     7081 aatgatttgt ttgtatggac tatctgtaca atcaccagct gcgaaaacgc cggggatatt
     7141 cgttgcgcca tttttctcgg tcactatttc atttcttgga ttcatttcga tcgttctatc
     7201 tagccaatcc gtattaggga gtaagccgac tagaatgaat actcctgaaa cagctagtgt
     7261 atgcatctcc atcgtacggc gatcgatata ggagagagat tctacctgat tcgtaccgct
     7321 gatttctttc gtcgctgcat gtttgataac agtgacattc gcaagagaga ctagtttatc
     7381 ctgtaagatt tgatctgctt ttaattctgg taagaattct agaacatata catgttttgc
     7441 taacccagcc aaatcgattg ctgcttcaac accagaatta ccaccaccga taacagcaat
     7501 ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc ctttattttt
     7561 aaactctttt tcgcctggta cattgataga acgccagtgc gcacctgtcg cgatgatggc
     7621 ggtttttgct ttaagtgata taccgttagc taagccaata ttaataaaat gttgctcttt
     7681 atttaaggaa acggcctgtt gattcatcat aatgtcgata gggtaactac gcacatgact
     7741 ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca tattttcaat
     7801 ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg acaagtcctg tacggattcc
     7861 tttacgtgct gcatagatag ctgcactact agctgctggt ccaccgccaa tcactaatac
     7921 atcataaggt tctttttcaa acaactcttc ttcaatcaaa gggccagtga ttttttcaac
     7981 aatttgttcg atcgtcatgc gtccgctact aaattcttct ccatttaaaa acacagttgg
     8041 cactgccatg atttttttat catcgatttc tttttggaac atcccacctt caatcatcgt
     8101 atgagtaata tttggattta atacagctaa tatattcaat gcctgtacaa cgtcggggca
     8161 attatgacaa gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta tttttttgat
     8221 tttaagactc aaagattcac tgattttcgg tgcccgtcca ctaatttgta acatagccag
     8281 taaaaaagag gaaaattcat gaccgagggg gataccagca aaaacgattc cactttcttt
     8341 gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt cccaagagat
     8401 tttaggagac atttctgcta tttccttcgt aaattcagct agtttttgac tattttcact
     8461 attatccaaa ctaacagaaa aaacaatagg agattcaatc agtgctaaat attgttcgag
     8521 attttgttta gtttctttgt ctaacatatc caccacctaa atctttccaa caagatcaaa
     8581 acttggtttc aatgtttcgc cactttcttt ccattttgcc ggacaaactt cacctgggtg
     8641 ggtacgtaca tattgagcag cgcgaatctt atcaatcagt gcactagcgt cacgaccgat
     8701 tccatctgca ttgatttcca tagcttgaac gattccgtct ggatcaataa taaatgttcc
     8761 tcgctgagct aatccttgct cttcatccaa aacatcaaaa atacgagaaa tctgatgaga
     8821 cggatcaccg atcatcacgt attcgatttt accgatagca tctgatgtat catgccatgc
     8881 tttgtgagta aagtgggtat cggtagagca agaaaagact tcaacaccta aagatttcaa
     8941 tgtactgtat tgttcttgta agtcttctaa ttcagtagga caaacaaaag taaaatcagc
     9001 tggatagaag caaagaatac tccagttccc cataagatct ttttcactga cttctaaaaa
     9061 ttctccttga tgataagcat gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat
     9121 agtaaaacct ccaataataa ttatttattt ataattattg taatttagtt ggagttgcaa
     9181 agtcaatgga tattttgtta tttaataaag attactcaaa aacgaaggtt aattataaaa
     9241 atagaagagg tcgcaagaaa atgctactgc tggaaatact cgggataacg ttgagtaaca
     9301 gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg agcttcttct
     9361 ggttttttat caactaaagc cttgagtaaa tcataatgtt gttccatgat cgtttcccat
     9421 ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt attatggtca
     9481 tctaaccagt tccaaacttc ttttcttcct gcaatttcga aacaagcttc atgaaataaa
     9541 ttatctgcac ggaaaaaagc gcgttcgtca tttattttga ctgctttttc ttgttcttct
     9601 aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg
     9661 atttgctctt ccaactgttc tcggacaaag cgagcatttt ctgcagaaac aagattaatt
     9721 tttgaaacat acgtaccact ttgaggtatc gtatacacga gttcttgctg acgtagttga
     9781 atcaatgctt cgcgaatggg agtccggccg atagcaagat gttcctccag tgccttttca
     9841 gatattttcc ttccaggttc taagtcagca tagataattt gtttacgaat cgcttgataa
     9901 gcttgagatt gcaagctgct tttcatcttt attcctccaa aatcatttct atgaacgtag
     9961 tataacagaa gaggtctgaa aaacaatctg gtatgtcaca ttaactagta aatttgtata
    10021 aatagtaaaa ttttggttga catagcgatt tttattgcat aaaattcact tgaatacaaa
    10081 aactaatatg ttagtttagg aggggaaaaa tgattgattt aaacaactgg aggaacttta
    10141 tccctgaatt atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca tcattaagag
    10201 aaaatggaca aaaaaatcct gtctggatcc atttcggtgg cggaaatctt tatcgaggat
    10261 ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga attgtccact ggtgtcattg
    10321 tttgtgaaac attcgatgaa gaaataattg aaagggctta ccaagatttt gatgatttga
    10381 ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa acgtatatta gctgcgacag
    10441 ctgacagtct gtattgtcat ccagattgtt ctgaacactt tgataagatg attcgacact
    10501 ttgaaaatcc tagtttacaa ttagttactt ttgctattac tgaaaaaggt tatcgattga
    10561 aagatacaaa taataaatat ataggaccga ttgcagtcga tataaaaaat ggtccaaaga
    10621 agccatcaca tacaatgagt attcttgctg cgctacttta taaacgtttt ttatctggag
    10681 cttggccgat tgcacttgtc tctacagata atttttcaca aaacggtagt caattacgta
    10741 cttctttgct aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa aatacattta
    10801 tccaatactt atcgaatgaa gaaattgttg cgtttccatg gacgatgatt gatcgtatca
    10861 ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt
    10921 cgattttcca aaccgcaaaa ggaaataata ttgctccatt tgtcaataca gaggacatcc
    10981 attatttaat catggaagat cattttccca atggacgtcc gaaattcgaa aaagctggag
    11041 tattcttgac tgatcgtgca acggtagata aagcagatac gatgaaggtg acgacttgcc
    11101 ttaatccttt gcatacgtct ttagctatta tgggttgttt actggggtat aggagcatag
    11161 ctgaggagat gaaagatcca gatttatcgc tgctagtttc tgaaatcggg tacaatgaag
    11221 gcctcccagt tgtggagtct ccaggtatca ttgatccaaa gatctttctg gaagaagtaa
    11281 ttactgtgcg cttttctaat ccaaatattc ctgatatgcc tcagcgaatc gccacggata
    11341 cctctcaaaa ggtggcgatt cgttatggtg agacgattaa aaaatatatg gaaatagaag
    11401 ttaaagaccc gcagacactt gtattcatac cattgactct cgctgcttgg cttcgatacc
    11461 ttattggcat tgatgatcaa ggaaaaccat tcatcccgag tccagaccca ctattgacag
    11521 actttcagca taagctttcc gatattcaac tgggtacgca agatacatca catattcatg
    11581 agaaaatggc tcctattttg tcaaataaag aaatttttgg tgtagatctt taccaagcag
    11641 agcttggcag aaaaatagaa gaaatgttca tcaagatggt agcaggaccg aatgccgtac
    11701 gaaaaacatt acaggattac ttacaagaac atggaggaaa tttatcatgg aaatgacttt
    11761 tagatggtac ggatcacagg aagaaaagat taccttagat gaaatcagac agattcctgc
    11821 ggtcaaaggg attgtaggca cattgatgga tataccagta ggagaggtat ggcctaggga
    11881 gaggatcaaa gaactaaaag aagaaattga gtctcgtggg ttaacactaa aagtaattga
    11941 aagcgtcaat gtgcatgatg ccattaaaat tggattacct tcccgtgagc agtatatcga
    12001 aaactataag cagacaatcc gaaacttggc agaatatgga atagaagtca tctgctataa
    12061 ttttatgcct gtatttgact gggtgaaatc tgaccttgac taccgcttag aagacggctc
    12121 atccacgtta gcttttatat ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg
    12181 catcgaacat tccagcaatg aatttgaatt gccgggatgg gaacctgagc gcttagctca
    12241 tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc gagaaaattt
    12301 tgcctatttt ctccaatcaa tcatcccaac atgtgaagag gtaggggtca aaatggctgt
    12361 acatccagat gatccgccat actcgatttt tggattacct cgaatcgtaa agaatcgaga
    12421 agatttagat tggttgtgcc gcatagtaga cagcccttat aacggaatca ctctttgtac
    12481 agggagtatt gcagaagatc cagaaaatga tgtatatggg attttagcag aattttgtca
    12541 aagagatcga atcccatttg cacatgttcg aaatattaaa tttttagcta atcgagattt
    12601 ctatgaggca ccgcataaat ccgaatacgg ttcattagac atgtaccgca tactaaaaac
    12661 aatgtatgac aacggattta acggatacat ccgtcctgat catggaagaa tgatctggaa
    12721 tgagacagga agaccaggat acggcttata tgatcgagca ttaggtgctg tttatctaaa
    12781 tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact
    12841 gtaattttat tatgtaaata agaaggatgt tcaaattctt gatacataaa tccggaggtt
    12901 gttaataagc attcggattt atgtatctta tttggtctag gaagtaactt gattttcttt
    12961 tttcgattga tccatgagtg cttcttcttt taattcaaag atatattctg agatcctcat
    13021 aatcactttt ttccagtctt ctggtgtgat gattcctttt aaaatataga tttttttgat
    13081 atgagaaggc aattgtttca aagggcagtc gctgataacg atgttgcagt tctccataaa
    13141 atcattttgt attttgatcg attcatttga aaaaatttgt tctatcttaa cgatcagatc
    13201 tttactgata taaaaatcac gcgtatagtt cacaccaatc acaattcgcg gctgtttttt
    13261 gtagcgatca tacaaatgta ctaggtcttc aaccatccat tgcacatttt ccttttctaa
    13321 aataactggt aacaagtgtt gattttgttc tctgaaatcc atataaaatt gttcaatggc
    13381 ggtttggact tctttcaaca cgctatgttc ttgataaagc atgaaagtag gaaggggcgt
    13441 atttggttga ttgaagttaa gaaatccatt ataaagttta tttcgtgcga atgccaatga
    13501 gtaaagaatc ttttcttttt caggtataaa caaatcaaat acagtacaaa aggactccac
    13561 catttctctt gaataaagaa catgaggaac gtttgctttt tttaaaagtt tgtattgctc
    13621 caagcttgtt tcttccgatt cgattttcgt gatagaaagt cgagctaaaa tattcactaa
    13681 gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag agggatcaat
    13741 aaaaaagtca atatattcat actctgtgaa ttctttttgt acgtctttca cagaagttgc
    13801 aggaaaattc tctttacata tttttaatag aaaatataat ttatctatca taccgctatt
    13861 tagtcggtca agtacttctt ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg
    13921 atcttcatca taaaattctg tagcagtact tgaaaagatg ttccaataaa tatctaatgc
    13981 tgcatattga atttgtacct cttttccaga aaaagacttt ctgccattat tagaaaagga
    14041 gatagacaaa ccgtacaatg ctaaaaatgc attaatcttt ttcattttcg tatacaaata
    14101 agaggcacta tagtttgttt gctgacttaa actgataatc gaatttgtct gacagctgaa
    14161 caaagaagtc aagatctgat aactgactga ttcgttgcag tatcttccaa atagacgata
    14221 atagcagtaa gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc
    14281 aatatgcagt tctgttagat tgagacgctc aaagtcttgt tctaattctt tgatgtatag
    14341 taaaaaattt gattcagaca tatggaaatg atctgctaaa tattgtgtag taataaagaa
    14401 acgatgttca gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagag
    14461 aaacgtttta atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta
    14521 aaacttgaaa atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag
    14581 gtagaatctc tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg
    14641 agatattcaa ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat
    14701 cagacagtcc acacagagga gatagaaaca gccaaatgga cagaaaccaa gtgggccaac
    14761 gtcaatcgaa ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta
    14821 agaaaaggac aaacagcgac catcaaagca aaaattgact attcaggaga cttagcacct
    14881 atactacaat cagctgttac atttgagaat gttgatcccg ctttgtcgat tggctatgac
    14941 gagaacactt tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat
    15001 caggacttgt taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat
    15061 catcatcatt taacgccgta tagtcagcgg cagacaattg agcaagattc agctatcgac
    15121 tcaggaggag atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa
    15181 gtaccgccta cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa
    15241 cccgatacag aaactcaacc agagccagac aatgaatcaa aacctgaaat aacccctgga
    15301 gaaaaaccag atgtagaccc agaagaaaaa ccggatgtga ctccagaacc agatacggat
    15361 tctggaaacc aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca
    15421 gaaaaaccag aggtagcccc agaagaaaaa ccagat
//
LOCUS       DACETN010000064        15315 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DACETN010000064 DACETN010000000
VERSION     DACETN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.066"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="IXF98_001846"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="IXF98_001846"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1556158.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IXF98_001847"
     CDS             complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IXF98_001847"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1556159.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IXF98_001848"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IXF98_001848"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1556160.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="IXF98_001849"
     CDS             complement(3374..4681)
                     /locus_tag="IXF98_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1556161.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="IXF98_001850"
     CDS             complement(4684..5265)
                     /locus_tag="IXF98_001850"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1556162.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="IXF98_001851"
     CDS             complement(5837..7129)
                     /locus_tag="IXF98_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1556163.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="IXF98_001852"
     CDS             complement(7126..7749)
                     /locus_tag="IXF98_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556164.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="IXF98_001853"
     CDS             complement(7752..8588)
                     /locus_tag="IXF98_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1556165.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="IXF98_001854"
     CDS             complement(8605..9492)
                     /locus_tag="IXF98_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1556166.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="IXF98_001855"
     CDS             complement(9508..10791)
                     /locus_tag="IXF98_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1556167.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="IXF98_001856"
     CDS             complement(11474..12157)
                     /locus_tag="IXF98_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1556168.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="IXF98_001857"
     CDS             complement(12175..12792)
                     /locus_tag="IXF98_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1556169.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="IXF98_001858"
     CDS             complement(12802..13626)
                     /locus_tag="IXF98_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1556170.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="IXF98_001859"
     CDS             complement(13619..14179)
                     /locus_tag="IXF98_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556171.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="IXF98_001860"
     CDS             14353..15309
                     /locus_tag="IXF98_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1556172.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4495 a         3417 c         2622 g         4781 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaa
//
LOCUS       DACETN010000065        15009 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DACETN010000065 DACETN010000000
VERSION     DACETN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.067"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXF98_001861"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXF98_001861"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1556173.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IXF98_001862"
     CDS             complement(1197..2216)
                     /locus_tag="IXF98_001862"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1556174.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IXF98_001863"
     CDS             complement(2849..3853)
                     /locus_tag="IXF98_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1556175.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IXF98_001864"
     CDS             complement(3866..4915)
                     /locus_tag="IXF98_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1556176.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IXF98_001865"
     CDS             complement(4932..5753)
                     /locus_tag="IXF98_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1556177.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IXF98_001866"
     CDS             complement(5740..6519)
                     /locus_tag="IXF98_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1556178.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IXF98_001867"
     CDS             complement(6536..7006)
                     /locus_tag="IXF98_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1556179.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IXF98_001868"
     CDS             complement(7018..7437)
                     /locus_tag="IXF98_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1556180.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IXF98_001869"
     CDS             complement(7518..10286)
                     /locus_tag="IXF98_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1556181.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IXF98_001870"
     CDS             10486..10710
                     /locus_tag="IXF98_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556182.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IXF98_001871"
     CDS             11489..13204
                     /locus_tag="IXF98_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556183.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="IXF98_001872"
     CDS             13207..14970
                     /locus_tag="IXF98_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556184.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4966 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgtttt
//
LOCUS       DACETN010000066        14873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DACETN010000066 DACETN010000000
VERSION     DACETN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.068"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            149..838
                     /locus_tag="IXF98_001873"
     CDS             149..838
                     /locus_tag="IXF98_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556185.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            844..1800
                     /locus_tag="IXF98_001874"
     CDS             844..1800
                     /locus_tag="IXF98_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1556186.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            1844..2356
                     /locus_tag="IXF98_001875"
     CDS             1844..2356
                     /locus_tag="IXF98_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556187.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(2612..3160)
                     /locus_tag="IXF98_001876"
     CDS             complement(2612..3160)
                     /locus_tag="IXF98_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1556188.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(3249..4430)
                     /locus_tag="IXF98_001877"
     CDS             complement(3249..4430)
                     /locus_tag="IXF98_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1556189.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(4666..6174)
                     /locus_tag="IXF98_001878"
     CDS             complement(4666..6174)
                     /locus_tag="IXF98_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1556190.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            6333..6524
                     /locus_tag="IXF98_001879"
     CDS             6333..6524
                     /locus_tag="IXF98_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556191.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            6727..7644
                     /locus_tag="IXF98_001880"
     CDS             6727..7644
                     /locus_tag="IXF98_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1556192.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            7637..8710
                     /locus_tag="IXF98_001881"
     CDS             7637..8710
                     /locus_tag="IXF98_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556193.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            8858..9376
                     /locus_tag="IXF98_001882"
     CDS             8858..9376
                     /locus_tag="IXF98_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556194.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            9357..9749
                     /locus_tag="IXF98_001883"
     CDS             9357..9749
                     /locus_tag="IXF98_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1556195.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            9825..10760
                     /locus_tag="IXF98_001884"
     CDS             9825..10760
                     /locus_tag="IXF98_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1556196.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            10893..11672
                     /locus_tag="IXF98_001885"
     CDS             10893..11672
                     /locus_tag="IXF98_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1556197.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            11665..11982
                     /locus_tag="IXF98_001886"
     CDS             11665..11982
                     /locus_tag="IXF98_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1556198.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            12226..13836
                     /locus_tag="IXF98_001887"
     CDS             12226..13836
                     /locus_tag="IXF98_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1556199.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            13993..14841
                     /locus_tag="IXF98_001888"
     CDS             13993..14841
                     /locus_tag="IXF98_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1556200.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         4803 a         2663 c         2926 g         4481 t
ORIGIN      
        1 gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
       61 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
      121 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
      181 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
      241 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
      301 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
      361 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
      421 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
      481 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
      541 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
      601 gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag aattgaaaag
      661 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
      721 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
      781 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
      841 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
      901 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
      961 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc
     1021 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
     1081 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
     1141 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
     1201 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
     1261 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
     1321 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
     1381 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
     1441 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
     1501 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
     1561 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
     1621 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
     1681 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
     1741 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
     1801 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
     1861 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
     1921 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
     1981 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
     2041 tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
     2101 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
     2161 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
     2221 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
     2281 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
     2341 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
     2401 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
     2461 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
     2521 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct
     2581 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
     2641 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
     2701 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
     2761 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
     2821 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
     2881 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
     2941 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
     3001 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
     3061 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
     3121 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
     3181 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
     3241 tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
     3301 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
     3361 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
     3421 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
     3481 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
     3541 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
     3601 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
     3661 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
     3721 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
     3781 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
     3841 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
     3901 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
     3961 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
     4021 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
     4081 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
     4141 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
     4201 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
     4261 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
     4321 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
     4381 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
     4441 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
     4501 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
     4561 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
     4621 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
     4681 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
     4741 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
     4801 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
     4861 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
     4921 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
     4981 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
     5041 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
     5101 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
     5161 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
     5221 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
     5281 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
     5341 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
     5401 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
     5461 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
     5521 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
     5581 atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct ttccacttcc
     5641 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
     5701 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
     5761 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
     5821 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
     5881 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
     5941 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
     6001 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
     6061 aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
     6121 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
     6181 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
     6241 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
     6301 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
     6361 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
     6421 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
     6481 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
     6541 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
     6601 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
     6661 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
     6721 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
     6781 aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
     6841 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
     6901 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
     6961 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
     7021 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
     7081 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
     7141 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
     7201 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
     7261 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
     7321 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
     7381 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
     7441 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
     7501 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
     7561 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
     7621 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
     7681 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
     7741 tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag aaaaagtaac
     7801 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
     7861 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
     7921 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
     7981 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
     8041 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
     8101 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
     8161 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
     8221 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
     8281 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
     8341 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
     8401 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
     8461 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
     8521 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
     8581 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
     8641 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
     8701 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
     8761 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
     8821 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
     8881 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
     8941 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
     9001 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
     9061 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
     9121 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
     9181 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
     9241 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
     9301 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
     9361 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
     9421 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
     9481 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa
     9541 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
     9601 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
     9661 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
     9721 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
     9781 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
     9841 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
     9901 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
     9961 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    10021 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    10081 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    10141 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    10201 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    10261 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    10321 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    10381 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    10441 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    10501 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    10561 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    10621 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    10681 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    10741 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    10801 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    10861 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    10921 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    10981 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    11041 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    11101 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    11161 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    11221 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    11281 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    11341 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    11401 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    11461 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    11521 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    11581 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    11641 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    11701 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    11761 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    11821 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    11881 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    11941 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    12001 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    12061 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    12121 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    12181 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    12241 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    12301 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    12361 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    12421 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    12481 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    12541 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    12601 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    12661 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    12721 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    12781 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    12841 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    12901 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    12961 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    13021 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    13081 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    13141 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    13201 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    13261 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    13321 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    13381 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    13441 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    13501 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    13561 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    13621 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    13681 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    13741 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    13801 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    13861 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    13921 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    13981 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    14041 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    14101 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    14161 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    14221 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    14281 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    14341 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    14401 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat
    14461 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    14521 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    14581 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    14641 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    14701 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    14761 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    14821 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tat
//
LOCUS       DACETN010000067        14293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DACETN010000067 DACETN010000000
VERSION     DACETN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.069"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            133..2604
                     /locus_tag="IXF98_001889"
     CDS             133..2604
                     /locus_tag="IXF98_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1556201.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="IXF98_001890"
     CDS             complement(2707..4443)
                     /locus_tag="IXF98_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1556202.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="IXF98_001891"
     CDS             complement(4440..5144)
                     /locus_tag="IXF98_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1556203.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="IXF98_001892"
     CDS             5275..5778
                     /locus_tag="IXF98_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1556204.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="IXF98_001893"
     CDS             5738..6076
                     /locus_tag="IXF98_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1556205.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="IXF98_001894"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="IXF98_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1556206.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="IXF98_001895"
     CDS             7627..8205
                     /locus_tag="IXF98_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1556207.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="IXF98_001896"
     CDS             8209..8730
                     /locus_tag="IXF98_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1556208.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="IXF98_001897"
     CDS             complement(8809..9363)
                     /locus_tag="IXF98_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1556209.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="IXF98_001898"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="IXF98_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1556210.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="IXF98_001899"
     CDS             9903..10154
                     /locus_tag="IXF98_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1556211.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="IXF98_001900"
     CDS             10279..11070
                     /locus_tag="IXF98_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556212.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..11761
                     /gene="rimM"
                     /locus_tag="IXF98_001901"
     CDS             11240..11761
                     /gene="rimM"
                     /locus_tag="IXF98_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1556213.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11751..12512
                     /gene="trmD"
                     /locus_tag="IXF98_001902"
     CDS             11751..12512
                     /gene="trmD"
                     /locus_tag="IXF98_001902"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1556214.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12648..12995
                     /gene="rplS"
                     /locus_tag="IXF98_001903"
     CDS             12648..12995
                     /gene="rplS"
                     /locus_tag="IXF98_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1556215.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13253..13546
                     /locus_tag="IXF98_001904"
     CDS             13253..13546
                     /locus_tag="IXF98_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1556216.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4794 a         2567 c         2905 g         4027 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    11521 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    11581 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    11641 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    11701 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    11761 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    11821 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    11881 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    11941 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    12001 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    12061 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    12121 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    12181 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    12241 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    12301 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    12361 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    12421 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    12481 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    12541 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    12601 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    12661 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    12721 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    12781 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    12841 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    12901 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    12961 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    13021 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    13081 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    13141 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    13201 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    13261 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    13321 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    13381 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    13441 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    13501 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    13561 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    13621 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    13681 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    13741 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    13801 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    13861 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    13921 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    13981 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    14041 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    14101 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    14161 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    14221 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    14281 aaaaagagaa aaa
//
LOCUS       DACETN010000068        14233 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DACETN010000068 DACETN010000000
VERSION     DACETN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.070"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(150..806)
                     /locus_tag="IXF98_001905"
     CDS             complement(150..806)
                     /locus_tag="IXF98_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1556217.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            999..1277
                     /locus_tag="IXF98_001906"
     CDS             999..1277
                     /locus_tag="IXF98_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556218.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1296..2504
                     /locus_tag="IXF98_001907"
     CDS             1296..2504
                     /locus_tag="IXF98_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1556219.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2652..3017)
                     /locus_tag="IXF98_001908"
     CDS             complement(2652..3017)
                     /locus_tag="IXF98_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1556220.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3164..3937)
                     /locus_tag="IXF98_001909"
     CDS             complement(3164..3937)
                     /locus_tag="IXF98_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1556221.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4111..4971)
                     /locus_tag="IXF98_001910"
     CDS             complement(4111..4971)
                     /locus_tag="IXF98_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1556222.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4971..6389)
                     /locus_tag="IXF98_001911"
     CDS             complement(4971..6389)
                     /locus_tag="IXF98_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1556223.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6446..6895)
                     /locus_tag="IXF98_001912"
     CDS             complement(6446..6895)
                     /locus_tag="IXF98_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1556224.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7340..7603)
                     /locus_tag="IXF98_001913"
     CDS             complement(7340..7603)
                     /locus_tag="IXF98_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1556225.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7702..8997)
                     /locus_tag="IXF98_001914"
     CDS             complement(7702..8997)
                     /locus_tag="IXF98_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1556226.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8994..10265)
                     /locus_tag="IXF98_001915"
     CDS             complement(8994..10265)
                     /locus_tag="IXF98_001915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1556227.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10430..11299)
                     /locus_tag="IXF98_001916"
     CDS             complement(10430..11299)
                     /locus_tag="IXF98_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1556228.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11591..13201)
                     /locus_tag="IXF98_001917"
     CDS             complement(11591..13201)
                     /locus_tag="IXF98_001917"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1556229.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         4061 a         2995 c         2428 g         4749 t
ORIGIN      
        1 aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
       61 tcaaaaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca
      121 aaacgctgat ttcacaacct ctttgttcat caaaagataa tattgtcttc taacaagtcc
      181 atattcaaaa taacgatatg atggtcttta acggtgataa taccatcttt tttcagttgt
      241 tttaaaattc tgtttacact gctagcagtt gagatgccac aaaatttggc gatttcttca
      301 ttcgtgatcg aaaaatcgat cagcatccct tcttctgtcc gcacaccgaa taaatcatat
      361 aattcgtaaa gttgcgtaca aacagctccc agctttccgt ttaatagcat ttgctgcatt
      421 tttttcatcg aataaagcag acgatagcgg taatactctt ttacatagtg ttgtagcttt
      481 gggtcttggt tgatattttg ccaaaagcgc acacgattga ttcgataaaa ctctgcatat
      541 tctgattcaa ctcgaatatt atacggcgaa tcgatgaatt gtgaatattc gtcttttaaa
      601 agagagacga tttcaagatc tttgatatag cgaaggttaa attctctccc atcatttgaa
      661 ataacacttg cttttaccgt tccctctttc aagatatacg tatgggaatc ttccatccct
      721 tcgaatgtca aatatttatg atatcctttg cgtataatcg gaaaatcatt tttatccaaa
      781 tattccttta agactctact atccaatgat gctcccccat ccaaactttc atctaaaaag
      841 atcatacagt actttaaaac tttttaaagt ccaaaggtat tttactaatg atattaacat
      901 ttgttaagta cctttttcca tcacttatgc gatttttttc ctttttttta ttcgttatac
      961 tgcagaagta aaatttttta agaatagagg gaaaaaacat gaattactgg atgaacacaa
     1021 tcattaatcg attagaaaca gcatatcaaa caagatttga tatgaaagct tctttagtct
     1081 ttttaaatga tgcgtatcaa aactcgatcg aattgatcaa agcagtggat gagaatccaa
     1141 caaacgaatg cgaagaattt ttaaatctgt ttatgtctac aagagattta tttattcgcc
     1201 aacttgttga tcgctatcca tccaattatc acgatgtaga agttcaaata caaaaattaa
     1261 aagcatattc agcttaaggg gaatgtataa catacatgga aaagaaatta tttaataaat
     1321 attttattgg aatcacattg atcaatttta ttgtctacct gatttattat ctgttgatgg
     1381 tcatcatggc agtcattgcg caagacacct tacacgccag cttgagccaa gcaggttttg
     1441 cttctgggat ctatatcatc ggaacattgc tggcacgctt attcatgggg aaacagttag
     1501 aactatttgg tcggaagttt accttaagag gcggcgcgat cttctatctc ctttcaacat
     1561 tagcatatct ttatacgcca actatcgggg cattgtattt agttcgattc ttgaatggat
     1621 tcggctacgg tacagtttct acagctacaa atgctatcgt tactgcatat atccctgcat
     1681 ctaaaaaagg ggaaggcatc aattattatg gattaagtac tagtttagct gccgctgttg
     1741 ggccattttt aggaatgatt ttgctgaata tgactgattt tcgtttcatt atctggtttt
     1801 caattgtact ggtcttcttt gttacgattg cctgccttgt ctttcctgta aaaaatattc
     1861 cattatctga agaacaaaaa atatctcttc gctcatggaa tatcaatagt tttattgaaa
     1921 agaaagcact tttcattaca ggaattgcct ttttgatggg tctttcttat tcaagtgttt
     1981 tatcattttt atcttcttat acaaaagtaa tccacttagt tgctgctggg tcatttttct
     2041 tcgtcgtata tgctttggtc atcacattta cacgtccatt gactggaagg attttcgatg
     2101 tcaaaggaga acaatacgtg atgtatccta gctatttatt cttaacagca ggattatttt
     2161 tactgagtgt aacgacgaat agtgtgacat tgcttatctc tggagcatta gtcggcctag
     2221 gttatggtac gtttatgtca aatggacagg ctgtttgttt aaaaatcgtc cgcgaacatc
     2281 gaatcagtat tgctttatct acctacttta ttggtttaga cctaggctta ggcgttggtc
     2341 cttatttgat ggggatgcta aaatctctta cttcatttag aggattgtat gtgattgccg
     2401 gagtcattcc attgatatgt acagcactct atcttgttaa tggccgaaaa catagtcatg
     2461 aagcagaggg cgaattgatt gaatcattga aaaatgaaat ttgatcttga tgaatagata
     2521 aaaaaaaccg ttcagatctg aaaataatct gagcggtttt ttgagtaaaa atagtttatg
     2581 attattctta attaaacatc tatttatttt taaagctttc atttatcgat aagaatagtt
     2641 taactgttga tttattccat tttccgttca tctggtacga tgccttgact tagcaaccac
     2701 ttgtggaaga accattctgc cacaccgatc aatagagcag gaatcaatac tttcatccaa
     2761 atatcgactg tatcattcat aaaatataga tagagccaca agataactgc agctgaacca
     2821 gcatctgcga tcgtcgctgc gatgttccct actttacgta agatcaacag atcccctagc
     2881 acatagatca tgactacaat aattacccca acaatcgttg aatccataaa gctaacaccg
     2941 tataaacctg tcagcacgac ccacagaatc gcccagatca tgattgcctt cactaccaac
     3001 gccttcacat gtttcatttt ctgcactcct ttctcctgga taaaaaagta agattatcta
     3061 acaagataac cttttcatta ttataccatc ggctgagaga agcgagcaaa gataaggtcc
     3121 tatctaaaaa tgaggaagcc acttagaaca ttcacgtaat aatttatccg tacagacatt
     3181 cgatatggtg ttcctttact ttcgcatact gttcttttgt cagttcttta cttgtcacca
     3241 gataatcgat ttcatgaaaa gcaatggctt gatgcatgaa tttatgtgca attttttgct
     3301 gtgtaactaa aacggcaagc cggtctgatt gttttctaaa agcttttttg accattgcct
     3361 ctccttcatg agtttctgtt actccttggc tgaaatctag cccgcgacaa ctcattatcg
     3421 ctaaatccgc ataatatgac aatgcatcgt tataggcttt tgaaccgttg attgttgctc
     3481 gtttcggaac aattgcccct cccaatacag atacttttgc cgttgtatat tcactcaaca
     3541 tagcagctgt gacgatgcca ttcgtaatga tctgcaaacc atcaaatctt gctaggtttc
     3601 tgactaagtg taaagaggtt gtactggaat ccaagaataa gaccatatca tctcgaatca
     3661 aagaagaagc tgtatctgca atcttacgtt tttctgtttc ttcttctaat ttggtgatct
     3721 tcatcaggta aggcacgtca atttttcgat tgtcaataag catggcgcca ccgcgaactc
     3781 tacggatcaa tccacttttt tccagaaaaa caaggtcacg tttaatcgtg gcttcgctat
     3841 aatttactaa ctcgatcaaa tcggtgactc ttgcaaattg gttgtttttt agataattca
     3901 caatctcctg ctgtctattc tccacttcat tcaacataaa atcaccccat ttactttata
     3961 tagtaacagg aaaaaagagg ttgtgacaga agtgattggc ttcgagaaat aagaagaaat
     4021 ttttcgaaaa aactacttct gaacatcgtg tgtggttaag agggtatgac aagactattg
     4081 tcataccctc ttgaccacac atttttattt ttagtataaa ctagccttgc ctactgtgtt
     4141 caaaatttcc attttatgtt tgaccacttc ttgtaccttt ttattagctg ctggataaat
     4201 tgcattcggc tcgtacatcg atggattttc actcaatatt tctctcaaag cattgaaaaa
     4261 gacgctttta agatctgagc tcaaattcac tttggcaact ccgtatttta ctgcttcgct
     4321 gacttctgcg tccggattac ctgaaccgcc atgtaaaaca aatggaagat cgatttgttg
     4381 gttcaattct ttcagtaagt cgatatttaa ttttggagtt ctgtttttcg gatacaagcc
     4441 atgcgctgtt ccaattgcta cagcaagtgt atcgattcct gtttctttca caaaaatctt
     4501 cgcttgatca ggatctgtat aaatgattgt ttcagcacct ccttcagcag aaccattatt
     4561 cccaatcgtt ccaagctcag cctccactga gacatttacc gcatgagcta acgcaaccac
     4621 ttttttcgta atctgtacat tgtcgtcgaa tgatttcaaa gacgcatcga tcatcacgga
     4681 agaatagccg tttcgaatcg cccgtaagac atcttctatc gtaccgccat gatccaagtg
     4741 gataacgatc ggcagtttac ttgtataagc ataggctctt actgccgcaa caaactcatc
     4801 acttaaatag gcgatttcat caggatgaat ttctaagatt actggtgctt ctaatctctc
     4861 tgcttcttcg atgattgctt tcatcatatc atagctgcaa atattgaagg ctggtactgc
     4921 aaattgattc tctttagcca ctttcaataa ctctttcatt gtatataaca ttacgcttcc
     4981 acctcatttg ttagattttc atttttcgtc cacttgtctt gttttttctc tttcactgtt
     5041 tttttcaata gtccaagcat aaaggctgta gtgactgtac ctgatgcaat tgccaagaca
     5101 tacataggta aattgttcat aacatttgga attagcatca cccaaatccc tccatgaggt
     5161 gccattgaag taactcctac tgccatggaa atccctgcag cgactgctga acctgtcatc
     5221 aaactaggta tcacgcggat cgggtcagat accgcaaacg gtatagcacc ttctgtaata
     5281 tacgaagcgc ctaaaatcca gcatgattga cctgaagtcc gttcttcttt ggtaaattta
     5341 tgtttgaata ataaagtggc taaagcaata cctaggggag gtgtcattcc tgcagccata
     5401 caagcagcaa tcggtgcatt tactccagct gacatcaaac cgatcgagaa agtcgaagtc
     5461 gatttattga tcggcccacc catatcagct gccatcatga tcccgataag taatccgaac
     5521 aatagcccgg atgtatttcc caagctattc agccaatttg tcaaagcatc caacacccat
     5581 gtgatcggtt gttcaatcac aaataccatc aaaagcccaa taattaagat cgataaaaag
     5641 gggacgacaa tcagctgata aatagcagaa aattcttttt ttacgctaat tttatttgcc
     5701 agaaaatcac agatataccc tgctagcacg ccaccaatca ttccgccaag aaaacctgaa
     5761 ccacctgtag aggccagtaa tccggcaaca attcctgggg caaaacctgc ttttccagca
     5821 atcgatgtag caatccccgc gcctaacgca ggaaccatca tggcaaacgc tgtatctcct
     5881 ccgatccttg aaaatgcagc tgctaacaca ttatagtctg ctgagttggg atcagccgct
     5941 gtaatcccaa aggcaaaact cagtgcgatg atgattccgc ctgcgatcac aaatggaagc
     6001 atgtaattca caccattcat gaaatgatta tagattgtcg gctttttctc atttgcagct
     6061 ggttcttttt cattagacac aacgaatacg ccaatatctc tcaatgcttc gtctattact
     6121 acttgcggtt ctttgattgc tcggcttgta gagacacgct ttactttttt cccggcaaat
     6181 cggtccaatt cgatttcttt atctactgca agaatgattg catctgcctc tttgatttct
     6241 tgttcagata actgattttc tgtttttaca ccttgtgttt cgaattttgc ttgatggttt
     6301 agatttcttg ctgttacttc taatttttct gccgacatat aagtatgagc aattccagtc
     6361 gggcaagcag ttacaccaac gattttcatg attattttcc tcctttttct tcactatcga
     6421 tactcggaca aagactctac tattttcatt cttgactaat ctgtctgatg atatctgctg
     6481 gcgtagtggc ttcttttatt ccctttctaa aatctgcatt catcaatcgt ctggagagtg
     6541 tgctgagaat ctctagatgc atatctgctg atttttcagg aactacgatg gcaaaaatat
     6601 attcaattgg catttcatct atcccgctat agtccgttaa tttggctgta tgaaaaaaac
     6661 acagtgctgg ttttgtcaca tatttgcttt ttgcatgggg aatcccaaat ccatcttcta
     6721 ttcctgtgga gatctcctcc tctcgtttga atagtgcctt aacgactttt tgaacatttc
     6781 ttaccttatt tgtttttttc aacacatccc ctattcgctc aaataatgat ccttttgaat
     6841 ctatcgatgg atcgaaaaca atcagttctt ctgcgatcaa ttgatcaaac ctcataaaaa
     6901 acctctcccc cacaaaaaat gttaacgttt tcttggaact gaggtcagta tagcatcgct
     6961 agctgtaccg ttcaatatta tgaatgaaca gaggaaaaag acccatcatc cgcgtgtata
     7021 agtcattaaa atgaacgatc tagctcacaa acaatcgcaa gtctataaca aaacaaaaag
     7081 ccttccttga aaggaaagct tagcttctaa ttttaggtaa atggtttttt acttcgaaat
     7141 aacccctctt catttcgtta atataaggtg tttttatcaa gtggataatt aaaactatat
     7201 actagttttt cattgttttg tgcattatga tcctacgact ttttcagcat caaacgaagt
     7261 tttaaattgg caaaaaacat aaaaaaacag actatcccta tcagatagtc tgctaaacgt
     7321 tgagttatca gcgtttttat tattccgcag cgtttgcgtc tttgagaccg tattttttgt
     7381 tgaaacggtc cacacgtccg tctgcttgtg tgaatttttg acgtccagtg tagaatggat
     7441 gtgagtcaga agttacttcg acacggatga ctgggtatgt attaccgtct tcccattcaa
     7501 ctgtttcttg tgaagttttt gttgaacctg acaagaattt gaaaccagtt gttgaatcca
     7561 tgaatactac tgggtggtaa tctggatgga tgttttcttt catgattttt tcgctccttt
     7621 gccctgctcc atttgctgaa ccagagttga tttgtcgatt tacaacatta acatcttaac
     7681 atgtttcagt caaggttgca attatctttt cagatttctg gtcggatttt ttttaccaaa
     7741 ggagacatct tgaaattctt tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg
     7801 gatcagttgt tccgtatatt ctaatgaatc tccagtcata ttttttcgaa gtttccaaat
     7861 ttcttctaat tcttcacttg gcatcaataa ttcttctttt cgggtacctg attttttgat
     7921 atcgatggct gggaagatcc gacgttcaga taattcacgt gaaagttgta attctagatt
     7981 tccagttcct ttgaattctt catagatcac gtcatccata cggcttcctg tatcgactaa
     8041 agcggtcgct aaaatcgtca aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa
     8101 tttcttaggt ttatacaatg cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg
     8161 tggaacgact aagttatagg cccgagcaag tctagtaata ctatccatca agataacgac
     8221 atctcgttta tcttcgacta aacgcattgc gcgttccaat actaattcag aaactcgagt
     8281 atggttttgc ggctgcagat caaatgtaga agataccacg tcgcctttca cgcttcgttc
     8341 tagatccgtt acttcttccg gtcgttcatc aatcagtaga acgattaatt ctacttccgg
     8401 atagttttcg gtgataccat ttgcgatttc ttttaataca gaagttttcc ctgcttttgg
     8461 cggtgcgacg atcaatccac gctgaccaaa accgattgga gaaaagatat cgatcattcg
     8521 ggtagatagt ctttgcggtg ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt
     8581 taaggctgga aaatgcggac gttcttttgc ttcttccgga tcttttccat taacgctttc
     8641 cacgtgcatc aaaccataat aacgttccga ttctttaggt ggacgggctt ttcctgccac
     8701 tttgtcccca tttctcaaac tgaatcgtct gatttgtgag gcagaaatat agatatcttc
     8761 tgcacttggt ccataattga ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc
     8821 caatattcct tccatataat aaaatccttg tttctctgct tgtgcacgaa tcacagctag
     8881 agataattcc tttttattca tcttactgta ataaggaatt ttaaattctt tggcataact
     8941 ataaacttct tttaacgttt tgttctctag ttctgccatt gtcaaataat cgctcattca
     9001 gctcccactt cctctatcat ttcaattgat gcccctaagg ctgttagttt ctcgataata
     9061 tgatcataac cgcgtaagat ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat
     9121 agtccagcaa tcactaaaca agcacctgcc cgaagatcag aagccacgac ttctgtgccg
     9181 tgaagtttat ttggaccatt cacgatgatc atatttcctt ccacggtaat atcagcgccc
     9241 attcgtgcta gttccggcac atggttcaca cgtttcgcat agatcgtatc gatgatttct
     9301 gaagtgcctg tagtcatcaa tagtaacgca gtaagtggct gctgcagatc tgtggcaaat
     9361 cctggataag gataggtcat gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg
     9421 atttcatctt cgctgatttt catatggaca ccaatttcct gcaattttgc tataaagctt
     9481 tccaaatgtt caaagatcac atttttcact ttgataccat ttcctactgc agctgctaat
     9541 gctagatagg ttcctgcttc gatgcggtca ggaatcatga aatgccgaca gccatgtaat
     9601 gtttcgactc cttcgatacg gatcacatct gtacccgctc ctcgaacttt tgcccccata
     9661 ttattcagta aagttgccac atctatgatt tccggttctc gtgcagcgtt ttcaattact
     9721 gtctgtcctt ttgcttttac agcagctaac atcacattga tcgttgctcc gactgaaacc
     9781 acgtccatga aaatccgatt gccacgcaat cctttatttt ctgtccgtaa atacatggca
     9841 ccatgttcat ttgtcacttc agcacctaga gcttcaaatc ctttgacgtg taggtcaatc
     9901 ggtctagggc ctaaataaca gccacctggc aggcctacta ccgcttcgcc aaatttacca
     9961 agcagtgttc ccataaaata ataagaagca cgcaaactat tgatttttcc gtgcggcatc
    10021 ggaatcgata cgacttctcg aggatcgatt tctagtatgt tgttggaaaa atggacagtt
    10081 gcgcccatga tctctaaaat ttcaattaat gaatgaacat cttgtatgtc cggcacacct
    10141 tccaagatca ctggggaatc agctaagatt gcagctggaa tcaatgcgac tgcactattt
    10201 tttgcaccgc tgatcgttac ttcaccagct aaaggtcggt ttcctttgat tacgattttt
    10261 ttcatctgtt tccaccttgt ttattaatct tttttcatgg atatgcttgt attttatcat
    10321 agataaaaaa aagaaacgag catggtctgc cgttttaagt aaaaaagaat ggagagcgac
    10381 ttcaaaagtc gttctccatt cctatatatc gtatctgtta tgtggatgct tacgctttgt
    10441 tagctgaacc gaaccagtcg atatgagatt tagcatcttc aacgattgct gctttacctg
    10501 gagctaataa tttacgaggg tcgaatcctt taccttcttt atctttacca gcttcgatat
    10561 atttacgagt agctgcagca aatgataatt ggaattcagt gttaacattt acttttgaga
    10621 tacccattga gatagctttt tcgatttgat cttgaggaat acctgatccg ccatgtaata
    10681 ctaaaggcat gtctggacct acagcatcag caattgcttg taagtggtca aaagctaatc
    10741 ctttccagtt ttctggatat tgaccatgga tgttaccgat accgcaagct aagtagtcga
    10801 tacctgtagc aaccatttgt ttgcattctt caatatcagc taattcgccg ttaccgatga
    10861 ttccgtcttc ttctccgccg attgaaccaa cttcacattc aactgagata ccttttgcat
    10921 gtgctttttc aacaacgtct ttagctaatt tcaagttttc ttcaaatggt agatgtgaac
    10981 catcgaacat tactgaagta tagcctaatt cgatacattc caaagcagct tcgtaatcac
    11041 catggtctaa gtggatagca actggaacag tgatgttcat tgcttcaact aaatcagtga
    11101 ttaaatcttt acagattttg taaccaccca tgtatttagc tgcacccatt gatgtttgga
    11161 taagtactgg tgcttgtttt gcttcagccg cttcaaggat tgcttgagtc cattccaagt
    11221 tgtttgtgtt gaatccgccg acagcgtaac cgcctttacg agcagctttt aaaaattcag
    11281 ctcctgatac tactggcata ataaaattcc tcctaagttt tgtaaaacta aatttgttaa
    11341 gacaattctt aaccaacgac cctattttac caaacatttc ggtagttttc tactaaaatc
    11401 aatgaaaatg aaaattttca agaagttttt tgcaaaaaat aaaatatcca atagcgcttt
    11461 aatcgaaaaa agtcttttgc tttctatctt ttttcctaca aataataaca aatcagaaaa
    11521 agagaaaagt gacaagaaaa ttgcttatcc ccttctcttg ctacttttct ctttctatca
    11581 agttcaaaaa ttattttgtt tcgtgatctg ctaatgatgc tccgacaaat gctttgatta
    11641 atttttgcgg gcgatttgga cgagaaatca attctgggtg gaattggcat cccacaaaga
    11701 attttttctc tggcagctct acgatttcta ctaaacggtt atctggtgag acacctgaaa
    11761 agactagccc ttgttcttca aacaattgac gatatttgtt attgaactca tagcggtgac
    11821 ggtgacgttc ttgcacaaca tcggctccat catatgcttc agccgtcttc gttccttttt
    11881 tgattttaca tgggtataat ccaaggcgta aagttccacc caaattctcg atgttttcct
    11941 gatcagccat taaatcaata atattgtttg ctgtatctgg attcgtttca gctgatgacg
    12001 catcttctaa tccaacgacg ttacgtgcaa attcgacaca agccatctgc ataccaagac
    12061 aaatacctaa aaacggaacg tcattttcgc gagcataacg aatcgcttcg attttccctt
    12121 ctactccgcg atcaccaaat ccaccaggaa ccaagattcc atctgcgtct ttcaaacgtt
    12181 cttctacatt ttctctcgtt aattcttgtg ctttgatcca atcgatctcg atgtctgaat
    12241 tataggcgaa accagaatgt ttcaatgctt caactactga gatatatgca tctggtaact
    12301 cgacatattt accgacaagc gcaatccgta cttttttctt caagttcaag acacgttctt
    12361 ctaattcttt ccattctgtc atgtctgctt ctggtgtatc tagttttaaa tggtcgcaaa
    12421 caatttgatc cattccttgt gcttgtaaaa ctaatgggat ggagtaaagt gtatctacat
    12481 ctggcgactc gataactgct tctggttcga catcacaaaa ttgtgccaat ttgtttttca
    12541 cgctttgaga aacaggttgc tctgtacgta ccaccaaaat attcggttgg atacctaaac
    12601 cacgtagctc ttttacactg tgctgcgttg gtttagtttt catttctcca gcagctttca
    12661 agtaaggaat caatgttgta tggatataca taacattatc tgtaccgaca tctgctttca
    12721 tttgacgaag tgcttcaagg aatggcaaag attctatatc ccctactgtt ccccctactt
    12781 ctgtaatgat cacatctgaa tcagtcattt tagctgctcg catgattttt tctttgatct
    12841 catttgtaat atgaggaata acttgaacag ttgctcccaa gtattcgccc ttacgttctt
    12901 tacgcaaaac ttcagaatag atttttcctg tcgtcacgtt ggagtatttg ttcaaattga
    12961 tgtcaataaa acgttcgtag tggcccagat ccaaatctgt ttcagcacca tcatctgtta
    13021 cgaatacttc tccgtgctga tatgggctca tcgtccccgg atccacgttg atgtatggat
    13081 caaatttttg gatagttact tttaagccgc ggtttttcaa taagcgtcct aaagaagctg
    13141 caacaatccc ttttccgatt gatgaaacga caccgccggt cacaaaaata tattttgtca
    13201 taagttcaaa aactcctttt taatttatgt gcaggaaaag tctcagacaa taaacaaagc
    13261 tccctattct tcaataagaa tagggagccg aaatttctgt aaacttctga ccctctaaaa
    13321 gggtgcccaa ggtgtataat acaagctgac taagggaaag tcaagtactc aaatgaatac
    13381 tatccgctta caattttttc tttttcgata tcctttgaga aattatttct taagtccttt
    13441 atgagtgcaa aaaaaattta acccacataa taagcttgat ataaacatag ctaacgttaa
    13501 acctaagcaa atcagaaaat cgttactgaa aaattttaag tgaaataata gataaaaaaa
    13561 taaaacaaac attatgatag ataatatccc acctaacaaa gaactacttt ttcctacttg
    13621 cagcaacagt ttatatagct tatcgtagaa acaatctttt tctttcaccc ttatttacct
    13681 cctttacgta aaaaacgtag tccacagttc tcggattttt ggcatatctt ttctattaaa
    13741 taatatcaaa aacaaaacaa ttccactaat tttttcaaag atgacctttt tttcaacaaa
    13801 agtgccttgt agggattcat tgaaggtaac gaatcacata agtaaaaaat aaggagatgc
    13861 gacaaaagtc gtgcctcctt atttctaaat aaacggtgtt ctcctgccaa ctcctcggtc
    13921 acaagtcaca atatccatca aacatgagaa gctgtttttg aaattatttc ttgtgattcg
    13981 gagttaaatg gtaggctcac aacctctgga taaacggtat tcaaaagcca gtttctcggt
    14041 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttaatact
    14101 cgaaactaaa cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc
    14161 ggtaataagt tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt
    14221 acttgttgca ggg
//
LOCUS       DACETN010000069        14211 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DACETN010000069 DACETN010000000
VERSION     DACETN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.071"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..407
                     /locus_tag="IXF98_001918"
     CDS             <1..407
                     /locus_tag="IXF98_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556230.1"
                     /translation="LSGCSSNKKSEAKEDNSLLQPGTVWKEEVGGLVNNLKIIDDTTW
                     EYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGIDLFIGKTALIVPYNKDGLETFK
                     FIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            404..646
                     /locus_tag="IXF98_001919"
     CDS             404..646
                     /locus_tag="IXF98_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556231.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            664..966
                     /locus_tag="IXF98_001920"
     CDS             664..966
                     /locus_tag="IXF98_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556232.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1036..3090
                     /locus_tag="IXF98_001921"
     CDS             1036..3090
                     /locus_tag="IXF98_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556233.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLALHDTEGKIEKTGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWTKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYLGLRTDKLKISKELNEQLNKLYQTT
                     TKVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRKKEKEHEQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFDGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESNMENSKESDSSKTKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3090..5699
                     /locus_tag="IXF98_001922"
     CDS             3090..5699
                     /locus_tag="IXF98_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1556234.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYSYAPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDAKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDQVRLKYDHQRKEYVAWYEFSQRELRDEEGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            5696..8092
                     /locus_tag="IXF98_001923"
     CDS             5696..8092
                     /locus_tag="IXF98_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556235.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            8144..8476
                     /locus_tag="IXF98_001924"
     CDS             8144..8476
                     /locus_tag="IXF98_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556236.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            8477..9070
                     /locus_tag="IXF98_001925"
     CDS             8477..9070
                     /locus_tag="IXF98_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556237.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9233..11257
                     /locus_tag="IXF98_001926"
     CDS             9233..11257
                     /locus_tag="IXF98_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1556238.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            11257..12462
                     /locus_tag="IXF98_001927"
     CDS             11257..12462
                     /locus_tag="IXF98_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1556239.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            12478..13122
                     /locus_tag="IXF98_001928"
     CDS             12478..13122
                     /locus_tag="IXF98_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556240.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            13133..13939
                     /locus_tag="IXF98_001929"
     CDS             13133..13939
                     /locus_tag="IXF98_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556241.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            13962..14192
                     /locus_tag="IXF98_001930"
     CDS             13962..14192
                     /locus_tag="IXF98_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556242.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
BASE COUNT         5219 a         2214 c         2971 g         3807 t
ORIGIN      
        1 ttttaagtgg atgttcaagt aataagaaat cggaagctaa agaagataat agtttattgc
       61 aaccaggaac tgtttggaaa gaagaagtag gtggtcttgt taataatttg aaaattatag
      121 acgatacaac atgggaatat tccgaaagtg tttggcatcc tgaacaagta caaataacag
      181 ttgagaagct aaaagactat aaaggattag aaagatataa ggtaatcgat tcaggtggaa
      241 tagatttatt cataggtaaa acagccttaa tagttcccta taacaaagac ggtttagaaa
      301 cgtttaaatt tattccaaca aacgaaaaag aacatcgaga taaaagtgaa atattaaaag
      361 aatacagtac taaatctgga tacaagttac aaaagactag cgagtgattg ctagtctttt
      421 tatttatcct gaaggaggaa tcacagtggc aaaagctaaa acgccatttg aaaaagccgc
      481 gtctaaaatc ttagaacgcc gcggtgtatc tgaaacacag tggaaacaag aagtccttga
      541 aaaagcacaa tttgacctat tacggggaaa agacaaggac ttagaaggat atatctatca
      601 acgtgaacgt gaaaagcttg ttttcgatga aattattaaa cactaaaagg agcgaacaat
      661 tttatgtttg aatcaattac aatgaatttt ttaacacaac cgaaagtagt agctgcggtt
      721 gatattttta gagcattaca aactgcagca actagtttaa aaaacaattt gatgattttt
      781 gctgctgctg cggccgttgc ttccattgtt attgggggtc ttttcttcct atttggacga
      841 agaggagctg aaaatgggaa gatgcacgta ggctatgtat tgatgggggt aggagttatt
      901 ttagcagcag cttctattgt ggtttcgtta tttggattgt ttggacaaac acctcaagga
      961 ttataagaaa gtaaaaaagt aggagtaaga agtagtcgat actttttact ccttttttat
     1021 gaaaggagaa tactcatgta ctttacgatt gcaggcaata ccaaaacaga taaagagtta
     1081 ataaaaaaag caacgaagga agtaggaaag cgtacttctt ttttaccgaa aacaattaaa
     1141 tattctgtca ttgaaacgga agaacagcta catgtgttag ggaaagtact ggaacacgaa
     1201 agtcgatctt ctgttaagtt gactctcgcg ttacatgaca cagaaggaaa aatagagaaa
     1261 actggtacag aatacgtttt tggcccactg gattatcaaa tttcagaagg tttctatcct
     1321 acattgatgg atttaatccg ccttgatgtg tttaaagaaa cggatgaaac tatgagtgtg
     1381 gaagagtctt atcgaaacga aaaactttta caagttttag aagaagagtt agagagcgac
     1441 caaagaaaag tgaatcaagg gagtgaagtc gaagtaaatg aagaagtacc atgggaacga
     1501 ccaatatttc caaagaagga gtcaaaagaa aaagagacag tctatgttga cgaacaagct
     1561 gatattccga ctcagaaatc gatatttgag gaagagaacg atgtgatggg tcaattggag
     1621 gtagatccct ttgaagaaac aatggaggat gaagagacgg ccgaagtaga agttgatcca
     1681 gttgaagcta atgaagagtc aacatggact gaaggggatt ctcaagaata tattgaacca
     1741 cttatcagtg aagaagtaga aaaaaacgaa atagagatac aaaaggcaac tgtatttgat
     1801 tatattttgg atcaatatcc gccagagcat gaatggacga aagaaaaaaa caaattattt
     1861 ttaaatgagc tttattcgaa atatcaatta ccccaaacct atcaagcatt tttagagagt
     1921 aaagaagaat taatctcaca gggtaaaggc acacttttag aacagttaga gtctgtccaa
     1981 tcaacaaatt ggcatgagct tgcacaagaa gatttattag ccacctttga agaacgtcaa
     2041 caagaaacag atcaagaaat taatgagtat gcgacggaac agtcgtctaa ttgggagaaa
     2101 gcaaaagagg aactaaataa agaagaacag cgagtgattg aagaagaagt acaaaaaatt
     2161 aaagagcgat acgaaaagaa acgcgacaat gcgtattcct tgtgtgtaga aaagatcaat
     2221 aattttactc agacgaatca agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa
     2281 cgtattaaag aacgaaaaga agaattttac ctaggtttgc gtacagataa gctaaagatt
     2341 agcaaagaat taaatgaaca actgaataag ctatatcaaa caacgactaa agttcttatg
     2401 gaaaaacacg aacagatcca gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag
     2461 tatgaaattc aacaacaaga attgaaagaa caagaagaaa aacaacggaa aaaagaaaaa
     2521 gagcatgaac aattagaaat aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg
     2581 atagaaaaac aacggttaga gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt
     2641 caagaacaaa tggatacatt gcgtcaagca cagttgaatt atctagcaca acctcaacca
     2701 gtagctgctg ttcaaacgcc agtgaacaac caagcaaatt taactccttc accacagcgt
     2761 tcctttgacg gatggatggt gggatgtatt gcaagtttaa gcttgcttct aggtggaggg
     2821 acagtatttg cttttaatca tatcaatcaa tcttcacagg ctgattctgt agccacaacg
     2881 gcgtcaatca aggaagaagc ccaggaacaa gcaagaaaag agtatgaaca acaattagaa
     2941 gaattgttga atcgtaccaa tcagtcaaca acagagtcaa atatggaaaa cagtaaagag
     3001 agtgattcta gtaagaccaa agaaggatca agcgattcaa ctgtacaatc aaacacttcc
     3061 gaaagttcag aatcaaatga ggtgaaataa tgcggattcg agaaacaacg aaacgaaaag
     3121 acaagccttt taatttagaa aaaaagaaat atctatcaca tttttatagc tatgcaccag
     3181 caggtgaaag attaccaggt aagcatgata agcgagtcaa acaaaaacta atgtatagcg
     3241 gtttagtcta tctcccttta gattatttag tgacaagaat acctgaagct ttcagtcgat
     3301 ttgaaatcat tcagggaagc ttacaaggag tgaatctgtt tcaattacag gaaatgccac
     3361 aggtctttca ttttgattca gctggttttt ggaatccttt gaccccagca acgagtttag
     3421 gtacacgctt agttgtttct acgatttgtg ttgttccagc tgtacttatg ggtttgaaaa
     3481 tggacatgtg gtttcgtccg ctagcggatg gacaaaaagg ggataatcgg ttaatgacac
     3541 taaaagagat tcaacaaatc tatcctcaaa taccagacgc gaaaggttct ttccctggat
     3601 atggtggaat tcctatcact cactataaaa aatattggta tatccaaaca gataccgtta
     3661 atacttgtat tgttggtact tctcgatcag ggaaaggaca gattgaagta ttagcaacta
     3721 ttgataattt gtctcgtgca gaaaaacaaa gttcaatggt tgtcaatgat cctaaaaatg
     3781 agttgtatgt agccagcaaa gacgaattag aagccagagg ctatgatgtt tatgcttata
     3841 atctaactga accgttacag tcaatgagtg ataatccttt ggctttaatt gtgaaatatt
     3901 ggcaacgagg agacgtggat accgctacgc aactagcaaa tacatttaca aatacgattt
     3961 attatgatgc caatgcttca gataacaaat actttaacga aaacgcgcag aaagctgtca
     4021 atgcgcttat ttttgcgttg ttagaacaag ccgatcaaac aggggattac tccaaattaa
     4081 cacccaataa tcttgttgag cttctttctg aaattggcgg ttttaattat caagatccta
     4141 ctaatgaatt taagcaactc aatgctttag atgaatttat gaatcagtta ccacctggaa
     4201 atctagcgaa aaaacaatat ggggcaacaa aaattggttc tgataaagca aaaggcaata
     4261 ttctatcaac agcgatcaca ggattgaatc catttaccct aacaaaaatt gcgaaaatga
     4321 cttctcaatc aaccattgat tataaatcgg ttggttttcc caaatatttg gatttgaaat
     4381 tacatgaaag tcttttaaat caacgaatcg tagtggaatt ttatcatcaa ggaaagaaat
     4441 ttcatgaaga acgagtaaaa gttggttata gaggtttttg tgaaatcaac tttgactgtc
     4501 atttagagca aggggatcaa gttcgtttaa agtatgatca ccaaagaaaa gaatatgttg
     4561 cttggtatga attttctcaa agagaattga gggacgaaga gggtaatatc gtttatcgaa
     4621 aaaaaagggg agaaacccat ttgccagaat gggaaaaaga agcggttctc acactagatg
     4681 aaagtaaaag taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg
     4741 ctatttttat gctcacacct gattatgatc catctaacca tatcattgtt agtatttttc
     4801 ttagtcagtt gtataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc
     4861 accgtaggat tcattttatt ttagatgaat ttgggaatat gccaccaatg gataatatgg
     4921 aaggaatcat gacagtcaca gctggtagaa acatgttgtg ggatctgttt attcaatcct
     4981 atcagcaact ttatgcgaag tatggggaac aaaacgcagc gattatcaaa gaaaattgtc
     5041 agacacatat ttatattatg tcgacagacg ataataccat cgaagaattt tccaaaaaag
     5101 tggggaataa gacggttgaa caagagaaca gtaccaccaa ttcattaggt atgaatactc
     5161 atatcaatcg caatgttgat tcagatcgta tcttaactcc tgaaagaatt tctacttttt
     5221 tagaaggtga aacgattgta ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa
     5281 tacggccgta tcctattttt aatcatgggg aaacgaatct tccttatgct tatcaatata
     5341 ttgaagattt caatccatcg tgggatatca atgatataga catacattgt acacatgcgg
     5401 acttggatct taaggcttta caaattgatt acgattattt tctacctgat atctatgcac
     5461 gtcgacttta tcacaatatg cacgatcaaa tagaagagga agctaaagaa aacattttta
     5521 ttaaaatcgt tcaatcttta gacaactcaa cggcacaaaa gttacttatt caattttatg
     5581 aagagaagca aatagatcgt ttgcaagaat accttgaaaa attgcatcaa aatgaagaaa
     5641 ttacagaggt tataaggaaa cgacttttgc tagaagctga aaaggcggtg aaagaatgat
     5701 cattggagga atattttccg gcattgccaa ctggtggtca ggcttatttg gggatgggga
     5761 tgcagatccc aaaaccgtaa cgaaagtctt acaagattat ggtaattatc tgcagtatca
     5821 agatgtattc agttggattt gtaatacttt tttatggggt ttgattaaag ggttatataa
     5881 tctcaatact ttactagaaa aaacaattta tcaaagcttt gatttaaaag acttattgaa
     5941 cgctgctggt gttaatgaac tgtttcaaag tttgattagt aaggtattgg ctcttttttg
     6001 tgtcgtcaca ttaatttatt taggcttaaa attttttatg agtaaacatc caccaaaaat
     6061 caaaaatatc ttggttaatc tatttatggc catgattttg atttttggtg gtagttcttt
     6121 aatcgatgaa gggctaaata ttagtaaaaa cttttacgga gacgtgactg cagcaaataa
     6181 agcagataaa actggatcac ctgcttttca aattattcaa aataatgtga tcgatgtgag
     6241 tacgctacta gaaactgatc cgaataaagt agaatcccta ccaacagata aaagaaatgc
     6301 tctcacggcg aaaaacttta agacagcaaa tattaattca gtgattattc ctaaggaagc
     6361 cgctaagatg gcggatgaag ataagagcgg tctatcagat atacaaaagg atcggctaag
     6421 agcgttacaa taccgactgg aactggatga taatggaaaa gaaatccctg tagagattag
     6481 tgatgagggg ttagcaaaat atgtgtatac ttcaggctat cgccgataca ttagtagtcc
     6541 agggattatt ttagctggtg aatcttcttt gtcggttgcc tatttattta tcttgtttac
     6601 gattgtaact tgcattattg agctggtatt taaaaaattc tatttagtaa ttgccgcgtc
     6661 aacggacttt gagacaggac aacggatgaa aagtgctata gaagatattt ctcaatcctt
     6721 cttgttattg gcgtttacga ttttagaatt acgaatctat acattaatgc tgtcaggtat
     6781 cggtgatttg cacgcagcag gcaagatcaa cggatttttg tatgttgtcg ctttaattgt
     6841 actaaccatt gcgctattca aaggctctca atctgttacg aagattttcg gtgttgatac
     6901 aagtctgaaa aacggcagca attctcttct tagcatgttt gccttaggaa atattgcgaa
     6961 aaatgtgggt agtggggcta aaaaccttgc aagtgctggt aagagtggtt taagcaattt
     7021 aaatgatatt cgtaaaaatg gatttggcaa tcgaaaagat gttattcctg ataacgggga
     7081 agaacaacca gacgataaag ctacaattgt acctaatagt aagaaaacag tttccgaaat
     7141 gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga
     7201 acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca ccttagaaga
     7261 gaaattagac ggcacgagag ctaaagaatt ggctgaagct gtgaagaatc catccgaagc
     7321 actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa ggactaaaag atgcgtatgg
     7381 tgaaggacaa gtcaatgcag cagtgaaggc aaatacaaca agtgaaaaag aaaatcaaga
     7441 aggtgcagcg attccttcag ccaaagatca ccaagatgag ttacattcta acgaaaattc
     7501 gatcggagaa aaagattcag aagaaggaca acatggacag ttaccaaaaa ccgatccaga
     7561 acaatatcgt tataaatcta acattaattc tgaaaatgaa aagcgcattt cagatttacc
     7621 aaaacgaaat caagaagtac aaacggatga ggattccatc gatttgccaa atgataatgg
     7681 aatcgaagtt ggaactgaaa aatcaatgaa aattggtttg cctaagacac aaaatttacc
     7741 aatgaaagat agtcgtttga atcaattcaa ggagaatgat caaagctttg ttcaccaatc
     7801 aactgcaggt actgttcaaa tgaaaccaag tcgtttgcct gaaaatggaa atctgacaac
     7861 tgtttcttac aatttatctg aaagtggtga gacagggaat gtgtccattc aaaatgttgt
     7921 gacaagagaa gctgcaccag aacctattca aaaagttcac acaggttaca aaccagcaaa
     7981 tgtggcattt actgcaccaa caaaaataaa tgtaacaaga gaaactaaaa acaggaacaa
     8041 tgaattgaaa gaacaactat tgagggatat caaacaggat atcaaaccat aaaaggccta
     8101 cgagttaggc cttttttcta tgtatgaaaa ggagaaatca tttatggata aatatattaa
     8161 catgaaaaat cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga
     8221 tctattaacg cttggtggtt gctttttttg ggcgaaaata gtcaataatc ttgtccactt
     8281 accactcttt tttgcactct tgaattatgg cttatggatt gcgatagctc tagtgattgt
     8341 ttggcgacct tataataacc caggtcaacg acaaattttt gcgattatcg aatctatttt
     8401 ctttgcggat aagaaccact atcatgcgtt agatcctaac cgaaattatc atggtacgta
     8461 taaggaggat gaataaatgc gagtcaaagg acaaaattca gttgataaga aatccatttt
     8521 gcagtttgga aaaccagaag aacgatctat cttggattta tttccttata aatcctgtac
     8581 agaagaggga gtcttagtta ctaaagaaga tcattttcaa cgttattatc gtgtagtttc
     8641 tacagacgtg gaaggattaa acgaacaaga gaaactagaa cgaatgaatc agttgaccac
     8701 cgtcatgcgt acgtatgtgc aaacaattaa gttagttagt ttgaccacag agacggatct
     8761 aacggaacaa atcgtacaaa agcggcaatt attagataag aatcgtatgg aacaatcctt
     8821 aggaagaaat ctacagcgat taagaaaata tgaaaagaaa ttagtagagg aaatccagga
     8881 attgaaacga gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa
     8941 gacaatcaag gatttacaaa ccagaaatcg tcagttaatg cgatctagtg gcattttagg
     9001 gttaaaacca ctctacaaaa aagaattgat caagattctt tatcgaatga acaatatgaa
     9061 tgatgagtag gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaatgatg
     9121 ttccgaagga agagatcact agaaaatttt ctcacacttt aagcctgggt aagttgggaa
     9181 aagggcaatc ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga
     9241 agcgattaaa acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca
     9301 actaggaaat cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa
     9361 acaagtgatg ccacaaggtg gattaaattt cgatgaagag tatgtgacct atggaaatgg
     9421 ttattcacga tgtgtgacag tctatcacta tacaaaaaat cctactcttt tttggttggc
     9481 caatctcatg aacaatcctc atacagtggc cacactagat gttagcacag atgaaaagca
     9541 aaaagtgatt gatcaaatca accgcgcatt agatgaatta aatgatcgat cagataatga
     9601 acgaaaagcg actgaccgaa ataaaagtgc ggaagaatac ctggaactag cggaatatgc
     9661 ccgaaaattg acttctaatg gggaaatttc taaacaagtc aaagtgagag tacatgttta
     9721 cggaaatacg atacaagaag tggatgaacg ggcgcaggaa ctgatcgaaa atctaaaagg
     9781 gatggattat cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt
     9841 taccatgttc gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc
     9901 gttaaacatt ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggaggtac
     9961 acaatatggg gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag
    10021 tgtacggttg tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt
    10081 tcttaaaatg atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa
    10141 gggaaaagac tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg
    10201 atctgacggg atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc
    10261 caggttatat gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca
    10321 aacgcactta gacaaagtag tctatcaggt acagttagtg agtcctcagc taaaaggaac
    10381 gatgaaagca gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc
    10441 acgtggcttt acttcctcaa attcgcgttc aaatactgat acgcaagtca caggtaaaga
    10501 tccagaagct tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc
    10561 aggtgcttcc caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt
    10621 atgtgagacg tatggcatga tttttgatgg tcacagtacg attcgacacc ttgaccaaca
    10681 acagttggtc ttttttgaca ttgactctat tacaggttta gatcgagggg tgcaacaatg
    10741 tcaaatgtac atggctatga cgttgatttg gaatcaggca ttgatccagg gaagaagaca
    10801 acgacgttta attaaagaaa aacaaatgga agaacaagac ttcaaacgct tcttggcgat
    10861 gattgatgag tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac
    10921 aaactttcaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc
    10981 acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt
    11041 tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga
    11101 aggactagtg ggtaaatcaa tgacgaactc agaattagaa tccattcctc aacaaaatca
    11161 aggagacgct gtgatcaact ttggatcaaa agaaaccatt cgcgttcatt ttagtccgaa
    11221 caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaatcaaga tgaaagttta
    11281 atcaagcagt atgttaagcg tcgcgcaaag cggcgcttat ttttgtggtt gtttggtact
    11341 tctgcaggtt tgattactat tttgatcact gtgtttgtta ccctatttct aatacttgcg
    11401 gcgggttcga ttgataattc ggattcagat tcaagcagtg gtggcgaagc ttttacagga
    11461 gaatattcgg aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat
    11521 gagattgcgc aatatgccgt aggtgcagca gtgaaatata aattattacc aagtgtgatt
    11581 ctttcgcaat acggttatga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc
    11641 aactattttg ggattacttg gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga
    11701 attggtggca ttgaaggtgg ctggtacatg aagtttccca actcaaaagc tgcattctcc
    11761 tattacggct ttatggtagc cacacaatca aattttaatg catgtgtagg aaataaaagc
    11821 cctggcgcaa gcttattgat cttaggacga ggtggttatg ccgctgcagg aattactgaa
    11881 gatagtccgt attatacagg ttgtatgtcg attattacct caaataaatt aacagaatat
    11941 gatgaatttg cgatcaaaca ttggggagaa ggcggaaatg acaacggtac gattactggt
    12001 gaatggacga atcctttccc tggcagcagt ttagataaaa gttccttcag cggtggtcaa
    12061 ttatttggaa cgaatccagg cggtgaattc cgtccgaatg gtttccatga tggattagac
    12121 tttggttcag ttgatcatcc aggaagtgag attcatgcgg ttcatggagg aaaagtcgtg
    12181 tacgttggaa atcctggtat ttcaggtttg ggcgcgtgtg tgatcgtgat taattacgac
    12241 ggcttgaata tggtatatca agaatttgca aatagtacag gaaattcccg tgtgaaagtg
    12301 ggagatcaag tgaaagttgg tcaagtaatc gggatacgag atacagcaca cttgcattta
    12361 ggcttcactc gaatggattg gcgtcaagca caaggacacg cattcacaga tgatggtaca
    12421 tggatcgatc cattaccatt tttgaatagc agcaaaaaat agatcaggag aaaaaatatg
    12481 gaaaataaaa cgttaaaaat cgtcttaaca gctgcactca gcattagctt aattggcaat
    12541 atcgcgctct tttggcagct gaaagaggaa caagcgacaa atacctataa aattgaagaa
    12601 gtggctaaaa acgcgtctga gaagctcaaa aaaaatttct ctgaggcaca ggcgaagatc
    12661 gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa
    12721 gacggtataa aagcaaaaga agaagtcaca gatgcccaaa gaaagacggc agaagagttc
    12781 ggtcaattgg ccattgataa atcactaagc agaaatgaat taacggaaaa actaaaagat
    12841 gtcgcaaccc aagaagtgat cgacaaatta agtcctgcag atgatgatca ccaacatgat
    12901 gattatggaa gttactcctt tacattaggt gacgtgcaaa cttacgctga aacaagcagt
    12961 gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa tcctcaattc
    13021 aaagatgtca aaccgcaaaa aataaaaggt ggattaactg tcactgaaaa acaagtagat
    13081 ggtcaatgga aagtcacaga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa
    13141 gaaaaaagga gcaagacaac gttttaaaaa acggaaaagt tatcttagat ggctgggaat
    13201 ctttacgatt ctcggctttt tgattcttgc tagtccgatt tatacgcata agaaggtaaa
    13261 cataccagat gaggtaagca cgaacgcttt tttaaacacg aaagaactga caatggtatc
    13321 aaaagaaaaa ggagcgaatc agcaattagt ttttgaattt gcgattgacg atgaggatca
    13381 aggcttatta aaagaactgg ctaatcttga ttatcgcgta gaagtaaaga cagctaaagg
    13441 tgattatcaa gcaataaaag ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa
    13501 attagcacat gtaccagaag aatatatcgc catgcgttta accattgttc ctgaaaaaat
    13561 tgatccaaaa gtggatatgc aagaaccaca agatctgatt tattacgttc acgaagataa
    13621 ggttaaggat caagtgaaag atgataatta tgaacaacat gcgatcaatt acaaagtgaa
    13681 aggatacaag aaagaacaaa aagcagcaca aaaacagatt gaatccttgc aagcaaccat
    13741 tgatttgaat gaagaattag tgaataaact aaaagaccaa ttgccttatc aagtcgcaga
    13801 tgatcaggaa aatactgaaa ataagattaa tgattaccag caagaaattg aaacatcgaa
    13861 aacgcaaatg aaagatcaag aagagatcat tcaaaaaatt caagaaaaaa ttgatcgtat
    13921 gacaaaagaa aatatttagt agaacaggtg gagtgaaaga aatgaaggga gcgaacttgg
    13981 tcggcaaaga tattttcgct gagcagaaag tattgattaa agcctatctc aaagttgtaa
    14041 aagctaaaga aatatgtcaa gaagtgaatt tatctaacac aacaatatct gagttgaaga
    14101 atggtagaaa gagtttagat accttaaaat tcagatcatt tgaagctttg tatttagctg
    14161 ctaaaaaata ttttacggct gaaagaaaat aaaaacagga ggattttatg t
//
LOCUS       DACETN010000070        14176 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DACETN010000070 DACETN010000000
VERSION     DACETN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.072"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(221..1177)
                     /locus_tag="IXF98_001931"
     CDS             complement(221..1177)
                     /locus_tag="IXF98_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1556243.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IXF98_001932"
     CDS             1281..1883
                     /locus_tag="IXF98_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1556244.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IXF98_001933"
     CDS             complement(1971..3470)
                     /locus_tag="IXF98_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1556245.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IXF98_001934"
     CDS             complement(3486..4010)
                     /locus_tag="IXF98_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556246.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IXF98_001935"
     CDS             4308..5057
                     /locus_tag="IXF98_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1556247.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IXF98_001936"
     CDS             5048..5833
                     /locus_tag="IXF98_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1556248.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IXF98_001937"
     CDS             5802..6518
                     /locus_tag="IXF98_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1556249.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IXF98_001938"
     CDS             complement(6620..7639)
                     /locus_tag="IXF98_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1556250.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IXF98_001939"
     CDS             complement(7658..8716)
                     /locus_tag="IXF98_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1556251.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IXF98_001940"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IXF98_001940"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1556252.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IXF98_001941"
     CDS             complement(10161..10997)
                     /locus_tag="IXF98_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556253.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IXF98_001942"
     CDS             complement(11181..12398)
                     /locus_tag="IXF98_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1556254.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IXF98_001943"
     tRNA            12781..12864
                     /locus_tag="IXF98_001943"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..14102)
                     /locus_tag="IXF98_001944"
     CDS             complement(13104..14102)
                     /locus_tag="IXF98_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556255.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4320 a         2872 c         2427 g         4557 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag
    13741 ttgaaatttt tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc
    13801 cctggagttg tcaaagagaa aattaattct gctaagctta ttattgaaaa tactgataac
    13861 ttataattat atcgtccatt gacaatcaaa tataaactaa ctatacttgt tagaacaaaa
    13921 aaacaggcgt tgacttgttc ttgattagtt aaaaatatca ataaaggaat acttacccaa
    13981 tatatttttc tatttatttc ttctcctctt aagagttgaa aaaacggata aaaagttagt
    14041 aaactaaatg ctactggcca ctgatagttc aaggtggttg ctctccaacc ggtttccccc
    14101 attactgtca atggtattaa acagtatata gagcaaacca ataataaatt tttaacattt
    14161 attttctcaa aaacat
//
LOCUS       DACETN010000071        14106 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DACETN010000071 DACETN010000000
VERSION     DACETN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.073"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            140..460
                     /locus_tag="IXF98_001945"
     CDS             140..460
                     /locus_tag="IXF98_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556256.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IXF98_001946"
     CDS             912..1112
                     /locus_tag="IXF98_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1556257.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IXF98_001947"
     CDS             1259..4048
                     /locus_tag="IXF98_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1556258.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IXF98_001948"
     CDS             4095..4622
                     /locus_tag="IXF98_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1556259.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IXF98_001949"
     CDS             4643..5833
                     /locus_tag="IXF98_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1556260.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IXF98_001950"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IXF98_001950"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1556261.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IXF98_001951"
     CDS             8224..8898
                     /locus_tag="IXF98_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1556262.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IXF98_001952"
     CDS             8895..9236
                     /locus_tag="IXF98_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556263.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IXF98_001953"
     CDS             9266..9625
                     /locus_tag="IXF98_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1556264.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IXF98_001954"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IXF98_001954"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1556265.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IXF98_001955"
     CDS             12541..14007
                     /locus_tag="IXF98_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1556266.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4677 a         2437 c         3017 g         3975 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagttt
//
LOCUS       DACETN010000072        13917 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DACETN010000072 DACETN010000000
VERSION     DACETN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.074"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            131..505
                     /locus_tag="IXF98_001956"
     CDS             131..505
                     /locus_tag="IXF98_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1556267.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXF98_001957"
     CDS             complement(619..1248)
                     /locus_tag="IXF98_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1556268.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXF98_001958"
     tRNA            complement(1382..1454)
                     /locus_tag="IXF98_001958"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IXF98_001959"
     tRNA            1668..1741
                     /locus_tag="IXF98_001959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IXF98_001960"
     CDS             1696..1845
                     /locus_tag="IXF98_001960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556269.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IXF98_001961"
     CDS             2338..3666
                     /locus_tag="IXF98_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1556270.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IXF98_001962"
     CDS             3868..4701
                     /locus_tag="IXF98_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1556271.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IXF98_001963"
     CDS             4717..5454
                     /locus_tag="IXF98_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1556272.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IXF98_001964"
     CDS             complement(5609..6577)
                     /locus_tag="IXF98_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1556273.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IXF98_001965"
     CDS             6809..7642
                     /locus_tag="IXF98_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1556274.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IXF98_001966"
     CDS             7676..8515
                     /locus_tag="IXF98_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1556275.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IXF98_001967"
     CDS             8541..9101
                     /locus_tag="IXF98_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1556276.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IXF98_001968"
     CDS             9300..11021
                     /locus_tag="IXF98_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1556277.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IXF98_001969"
     CDS             11186..12328
                     /locus_tag="IXF98_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1556278.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IXF98_001970"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IXF98_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1556279.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2298 c         2833 g         4034 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACETN010000073        13807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DACETN010000073 DACETN010000000
VERSION     DACETN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.075"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXF98_001971"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXF98_001971"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1556280.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IXF98_001972"
     CDS             complement(2260..2511)
                     /locus_tag="IXF98_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1556281.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IXF98_001973"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IXF98_001973"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1556282.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IXF98_001974"
     CDS             3461..3679
                     /locus_tag="IXF98_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556283.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IXF98_001975"
     CDS             complement(3968..4480)
                     /locus_tag="IXF98_001975"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1556284.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXF98_001976"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXF98_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1556285.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IXF98_001977"
     CDS             complement(6933..7367)
                     /locus_tag="IXF98_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1556286.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IXF98_001978"
     CDS             complement(7377..8165)
                     /locus_tag="IXF98_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1556287.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IXF98_001979"
     CDS             complement(8162..9103)
                     /locus_tag="IXF98_001979"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1556288.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IXF98_001980"
     CDS             complement(9280..9867)
                     /locus_tag="IXF98_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1556289.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXF98_001981"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXF98_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1556290.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IXF98_001982"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IXF98_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1556291.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IXF98_001983"
     CDS             complement(12784..13719)
                     /locus_tag="IXF98_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1556292.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3807 a         3095 c         2436 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cgtaatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACETN010000074        13538 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DACETN010000074 DACETN010000000
VERSION     DACETN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.076"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(62..1009)
                     /locus_tag="IXF98_001984"
     CDS             complement(62..1009)
                     /locus_tag="IXF98_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1556293.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1199..1468
                     /gene="rpsN"
                     /locus_tag="IXF98_001985"
     CDS             1199..1468
                     /gene="rpsN"
                     /locus_tag="IXF98_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1556294.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1644..2006)
                     /locus_tag="IXF98_001986"
     CDS             complement(1644..2006)
                     /locus_tag="IXF98_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1556295.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2017..3138)
                     /locus_tag="IXF98_001987"
     CDS             complement(2017..3138)
                     /locus_tag="IXF98_001987"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1556296.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3154..3504)
                     /locus_tag="IXF98_001988"
     CDS             complement(3154..3504)
                     /locus_tag="IXF98_001988"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1556297.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3634..5145)
                     /locus_tag="IXF98_001989"
     CDS             complement(3634..5145)
                     /locus_tag="IXF98_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1556298.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5467..6819)
                     /locus_tag="IXF98_001990"
     CDS             complement(5467..6819)
                     /locus_tag="IXF98_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1556299.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6972..8048)
                     /locus_tag="IXF98_001991"
     CDS             complement(6972..8048)
                     /locus_tag="IXF98_001991"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1556300.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8244..9989
                     /locus_tag="IXF98_001992"
     CDS             8244..9989
                     /locus_tag="IXF98_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1556301.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10238..10705)
                     /locus_tag="IXF98_001993"
     CDS             complement(10238..10705)
                     /locus_tag="IXF98_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1556302.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10689..12062)
                     /locus_tag="IXF98_001994"
     CDS             complement(10689..12062)
                     /locus_tag="IXF98_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556303.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12383..12871)
                     /locus_tag="IXF98_001995"
     CDS             complement(12383..12871)
                     /locus_tag="IXF98_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556304.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12893..13495)
                     /locus_tag="IXF98_001996"
     CDS             complement(12893..13495)
                     /locus_tag="IXF98_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1556305.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4279 t
ORIGIN      
        1 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
       61 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
      121 gtgcttggat gacatgctta agggaagcag acagattcat tgaaataaaa acatgaaagg
      181 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
      241 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
      301 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
      361 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
      421 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
      481 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
      541 acccgaccgc acccgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
      601 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
      661 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
      721 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
      781 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
      841 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
      901 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
      961 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
     1021 ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt
     1081 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
     1141 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
     1201 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
     1261 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgca aacttccttt
     1321 agatgctcgt ccagaacgat tgaaaaatcg tgatttgatc gatggacgcc ctcgcggata
     1381 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
     1441 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
     1501 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
     1561 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
     1621 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
     1681 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
     1741 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
     1801 tctattcctg ttccagtagc tttgatccca atatgagtcg gaagcttagg tttagccatt
     1861 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
     1921 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
     1981 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
     2041 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
     2101 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
     2161 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
     2221 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
     2281 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
     2341 gattccttca cctgctggga gttttttgac ttgaattaat tcagaaacca gctcaagtgc
     2401 tggctgcagt ggataaactt ccggtagtgc gtgcccagag ggattcagtc cgtacattgc
     2461 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
     2521 gctgacatgt atatatcgag gaagttcggg aagttttttt agcacttcta taaagcgttc
     2581 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
     2641 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
     2701 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
     2761 cgtctctagc ttacttttct ctaaaacagc agctgctgcc tgcaaccatt cccgtgtacc
     2821 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
     2881 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
     2941 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
     3001 atgaccgtat ccgtttgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
     3061 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
     3121 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
     3181 agctgtttca tttgtatgag taatactgac ccatactttg ccttcatgag gtgaacgcgt
     3241 cacaacaggc tgaccgtttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
     3301 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
     3361 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
     3421 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
     3481 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
     3541 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
     3601 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
     3661 tccgcgtttt ttatcgttgt gacgtttacc gccatctttt tggctgttat tatttttgct
     3721 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
     3781 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
     3841 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
     3901 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtactt
     3961 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
     4021 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
     4081 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
     4141 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
     4201 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
     4261 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
     4321 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
     4381 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttagttcag gtgtttgaac
     4441 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
     4501 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
     4561 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
     4621 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
     4681 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
     4741 gttgatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
     4801 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
     4861 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaactcac gtgttggtgc
     4921 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
     4981 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
     5041 tgctagtgga atcgtttctg attggatagg tgttgcttct tcaaaaccag cgcgttcgac
     5101 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
     5161 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
     5221 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
     5281 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
     5341 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
     5401 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
     5461 agatttttat ttcatttgct gaagctgttc taccacttca aataagccca tgctgttgct
     5521 gccttttagc ataatactat ctgttgcttc aagattttgt tttacagcat caatcaattt
     5581 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
     5641 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
     5701 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
     5761 taacacagcg attctgcgtc cttttgttgg tagattttta aaagtatcta ataccagtcc
     5821 catggctgtt ggatttgcat tatagacatc gctcaaaata gctgcaccat ttcctgccgt
     5881 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
     5941 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
     6001 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
     6061 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
     6121 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
     6181 tccgtctggt acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
     6241 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
     6301 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca agacgatctt
     6361 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
     6421 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
     6481 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcacctactt tttccaagta
     6541 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatc
     6601 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
     6661 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
     6721 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
     6781 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
     6841 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
     6901 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcttcttc
     6961 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
     7021 cctaattgaa tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
     7081 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
     7141 tcgtttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
     7201 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttccggcac ttcggctgga
     7261 atctgcattt cgattttatt attgatatat ttggcttcat aatcatagaa tgctacgtct
     7321 tttacgactt cgccaggcaa agtcgtccga acatcttcat ttcctaatac agcaacttcg
     7381 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
     7441 gctaaagcat tttgcagctc ttctcggttt tctgcctttg taatgccgac actagaaccc
     7501 atattcgcag gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
     7561 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
     7621 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
     7681 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
     7741 tccccgtttg gcccgtgtaa aactggaaaa acgatggctc cttcttcttt gatttctccc
     7801 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
     7861 tgcaagacat ctttgctagc aggtttttct gttagtaatg gacctttgac ccattgtcct
     7921 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
     7981 gaaaatgcgg aaagaatgga aacttcatgc tctgcgctgc gtccgccata tagtaaagta
     8041 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
     8101 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagttggt ttctaaaaga
     8161 acaaacgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
     8221 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgtagccgc ttggcagaaa
     8281 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
     8341 aacaggtaaa aaaagccttt gaagaactag gccccacctt cattaagata ggacagatgc
     8401 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
     8461 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
     8521 tttcattatc agatatattc gatgattttt ccaaatcgcc ttttgcctct gcttctatgg
     8581 ggcaggctca tcgcgccaag ctgaagaacg gtgactcggt cgtcgttaag atccaacacc
     8641 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
     8701 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
     8761 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
     8821 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
     8881 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaaccattta atgaacgccg
     8941 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
     9001 caaagctttt agttgagaac tttatgaaac aggtatttga tgatggtttt ttccatgctg
     9061 atcctcatcc agggaatctg ctattccatg tactaaaaaa agaagaacag acacaagcta
     9121 gccgaaaaac tgaaatagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
     9181 cttctacaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
     9241 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
     9301 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
     9361 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
     9421 ttgatgaaat caacttgcaa gaagtattca cacacgtcgt gacgatttgt catcaaaata
     9481 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
     9541 tgatccgcgt ccttgaccct gaagtttccc tcatggaagt cgcttcgcct tttgcccaac
     9601 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
     9661 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
     9721 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
     9781 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
     9841 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
     9901 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
     9961 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    10021 agaaataaga agtgattttg cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    10081 ttttcgaaga ggctacttct gaagcaccga gaataaaaaa tgtgaaaaag ctgttctgca
    10141 cccagtaata agaacaatca aaaaggaaca tggagcacat ttatcctgtt tccctgttcc
    10201 ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg
    10261 gtaaaccttg gtcaattgcc ttttttaacg cttgacaaaa agacatatga tctttctccg
    10321 taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac
    10381 gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca
    10441 ctcggacgcc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt
    10501 cattcttctt aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt
    10561 agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga
    10621 taaccacgtc aagaccatag gattctccta attttatgac atcttctttt actggagaac
    10681 catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt
    10741 actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca
    10801 ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc
    10861 atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct
    10921 ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc tgcatcagtt
    10981 tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag
    11041 acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc
    11101 aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt
    11161 gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg
    11221 tacattatgc caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt
    11281 agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa
    11341 acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt
    11401 tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat
    11461 aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa
    11521 cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag
    11581 cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag
    11641 atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga
    11701 aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt
    11761 gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca
    11821 ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg
    11881 actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc
    11941 caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta
    12001 cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac
    12061 atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag
    12121 gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaatacagaa agaagcataa
    12181 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    12241 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    12301 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    12361 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    12421 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    12481 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    12541 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    12601 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    12661 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    12721 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    12781 caatgatcgg cacaaaagct tccggccata cttcttgtat gactgccatc agctcatcca
    12841 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    12901 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg
    12961 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    13021 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    13081 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    13141 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    13201 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    13261 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    13321 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    13381 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    13441 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    13501 ttccccacat cttttttcat ttattaaaac tgcctcct
//
LOCUS       DACETN010000075        13050 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DACETN010000075 DACETN010000000
VERSION     DACETN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.077"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(79..151)
                     /locus_tag="IXF98_001997"
     tRNA            complement(79..151)
                     /locus_tag="IXF98_001997"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="IXF98_001998"
     CDS             532..1101
                     /locus_tag="IXF98_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1556306.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="IXF98_001999"
     CDS             1088..1948
                     /locus_tag="IXF98_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1556307.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="IXF98_002000"
     CDS             2138..2842
                     /locus_tag="IXF98_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1556308.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="IXF98_002001"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="IXF98_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1556309.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="IXF98_002002"
     CDS             4679..5995
                     /locus_tag="IXF98_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556310.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="IXF98_002003"
     CDS             5996..6865
                     /locus_tag="IXF98_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556311.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="IXF98_002004"
     CDS             6920..7729
                     /locus_tag="IXF98_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1556312.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="IXF98_002005"
     CDS             complement(7832..8461)
                     /locus_tag="IXF98_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1556313.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="IXF98_002006"
     CDS             8631..9923
                     /locus_tag="IXF98_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1556314.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="IXF98_002007"
     CDS             complement(9999..10661)
                     /locus_tag="IXF98_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1556315.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /locus_tag="IXF98_002008"
     CDS             10886..11170
                     /locus_tag="IXF98_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1556316.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="IXF98_002009"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="IXF98_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1556317.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4339 a         2254 c         2824 g         3633 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggtttttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct
//
LOCUS       DACETN010000076        12859 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DACETN010000076 DACETN010000000
VERSION     DACETN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.078"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            6..2378
                     /locus_tag="IXF98_002010"
     CDS             6..2378
                     /locus_tag="IXF98_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556318.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3899
                     /locus_tag="IXF98_002011"
     CDS             2439..3899
                     /locus_tag="IXF98_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1556319.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3910..5919
                     /gene="topB"
                     /locus_tag="IXF98_002012"
                     /pseudo
     CDS             3910..5919
                     /gene="topB"
                     /locus_tag="IXF98_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6265..6861
                     /locus_tag="IXF98_002013"
     CDS             6265..6861
                     /locus_tag="IXF98_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1556320.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6874..7773
                     /locus_tag="IXF98_002014"
     CDS             6874..7773
                     /locus_tag="IXF98_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1556321.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            7776..8260
                     /gene="ssb"
                     /locus_tag="IXF98_002015"
                     /pseudo
     CDS             7776..8260
                     /gene="ssb"
                     /locus_tag="IXF98_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8282..8539
                     /locus_tag="IXF98_002016"
     CDS             8282..8539
                     /locus_tag="IXF98_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556322.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8698..8832)
                     /locus_tag="IXF98_002017"
     CDS             complement(8698..8832)
                     /locus_tag="IXF98_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1556323.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            8975..9235
                     /locus_tag="IXF98_002018"
     CDS             8975..9235
                     /locus_tag="IXF98_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556324.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9684..9890
                     /locus_tag="IXF98_002019"
     CDS             9684..9890
                     /locus_tag="IXF98_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1556325.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            9890..10141
                     /locus_tag="IXF98_002020"
     CDS             9890..10141
                     /locus_tag="IXF98_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556326.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10157..10594
                     /locus_tag="IXF98_002021"
     CDS             10157..10594
                     /locus_tag="IXF98_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556327.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11275..11670
                     /locus_tag="IXF98_002022"
     CDS             11275..11670
                     /locus_tag="IXF98_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556328.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            11652..11918
                     /locus_tag="IXF98_002023"
     CDS             11652..11918
                     /locus_tag="IXF98_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556329.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            11928..12161
                     /locus_tag="IXF98_002024"
     CDS             11928..12161
                     /locus_tag="IXF98_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAQ1556330.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            12504..12686
                     /locus_tag="IXF98_002025"
     CDS             12504..12686
                     /locus_tag="IXF98_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1556331.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            12676..>12859
                     /locus_tag="IXF98_002026"
     CDS             12676..>12859
                     /locus_tag="IXF98_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556332.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEE"
BASE COUNT         4756 a         2107 c         2701 g         3295 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaatg atcaaatcga aaagatgttt gaacaccaaa
      121 gaaagaccaa cggtcaacca atgaacgata agagaaacgg gcattcttgg tttaggcaac
      181 aagaacgaat cgaaaataaa gtccaaagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggttataac aagtacggtg
      301 gcctggatat gacgatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gctgccacga tccgaaaata ccaacgaaaa ttggaaacat
      421 tagaacagct gaaagaaaaa tcagagaaag gaaaagaaaa tattcttcct gaagtacaag
      481 cgatcattga cagcggacga gtgacgcaat ggaaaaagaa tccaactatc tattttctga
      541 aaggctatcg aaaagtcgct ttagaactca atgaaaacgg gggatttgag gaatctaaaa
      601 aataccaagc cacgtcggaa gaagaaaagg cagctgttcg tgaattactt gaagaagcta
      661 cacctactga ttctgtgaag gagaaagaaa ccgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agcaaaaggg ttgaaaagtt atggtgggga gatcgtaaca ctagaattga
      781 tcgatcaact caaaacactg gataaccaat acgccgcaga agcaggctat tataaattct
      841 attttgaaga agtgaagaat ggagaagtag tagatcagat acgaatggat ctaggggatg
      901 gcgtcaatag taatcacccc atctatttag agttagctag attgtgcgaa caagtacctg
      961 agaaggaaca aaaacaaaca gtcaaaaatg aacctatttc aaaagtacaa aatgagatta
     1021 aagagacaaa aacaaaacaa aagggttatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc actttcccaa caggcgcttg atgcggtgaa aaagcatact cattcacctg
     1141 aagacgtcaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat cgaggaacaa tttccaggcg caacggcttg tttaggtcgt gccgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagag gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac aattgaagcc tgaagactat ccgaaaatct
     1501 tcccgaaccg cgtctttgat tttcaattgg atcaaacagg gcatacagaa cttcaagttg
     1561 ggattcaagc ggtagccaaa aatattggaa ttcaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacgtg gggcgtacgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagcc acaagtattc
     1741 acgaattagc ccatgctaga atgcacaaat tttcagaatt agatactgca acaaaagaac
     1801 tacaggcaga aatgacctcc tatattgtgt gcaagcattt tgggatggac acctcggaga
     1861 aagctattcc ttatattgcg acatggacga agaatggtca gacgttggat gataaggaag
     1921 ctgcggaacg tgggaaaatc atgaatgatg tcagtcgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccag ccagcagaag
     2041 tgaagcagga tgagaaaaat gttgtagaaa cagcagaaca aaagaatatt gtgctcgttg
     2101 gctcactttg gatcgacaag aaagacggga aagtcctgga agaagacacg ggccgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atacgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgagaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagattcaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatt
     2821 tgaaggaaga acgaaaactg cccgattggt tgattgatta tgcagaaaaa gaaggattga
     2881 tcgccgaact aaagccaaaa catgaacggc aaaatttctt agttcaagac gatcgtttgg
     2941 atcatgcggt cgccttttta tggaaagatc cacagacaaa agagacagta ggggcgagtt
     3001 atcaaggaac attcattgat tatgaacgtt ttggtgagag aggcacatat aagcatatag
     3061 ataaaaattc gacagcgaat catggattta acctaaagat cggtgaccct aaacagttaa
     3121 aattttttga gagtagtatt gatttactta gctatgctgc actgaatcgt gatcagttaa
     3181 acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt cattattttg
     3241 gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatt gagatttgtg
     3301 tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg ggcgcagttg
     3361 atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc
     3421 caaaagaata caaagtcatt tatgaagaag ttgcaaaaga aatgaaagtt gaacctgaag
     3481 caattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa ctggtttccg
     3541 ctcacaaagt aaatgcgtct tttggtcagc agctttctgt taacgattca atagaagcta
     3601 ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaaggttgt gaacgcgtgg
     3661 atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat gctcagattc
     3721 ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa catgaatttg
     3781 tacctgaagt aaaaaaggat tggaatgatc aattgaagca tgaaatccac caacaagaga
     3841 tcagaaagca aaaacgtgcg atgttgtttc aacaaggcaa acaacaagaa agggagtgaa
     3901 atcgtcatga tgaaaaccgt tattttagca gaaaagccgt cacaagcgaa agcttatgcc
     3961 gatagcttct ctaaagccac ccgaaaagac gggtattttg aaatccaaga tagattgttt
     4021 cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag tcgagttaga ttcgcccgat
     4081 atgtacgatg aaaattggaa acaatggtca ttagagcatt tgcctatttt ccctacacat
     4141 tatcactatc atgttcctaa agataagaaa aagcaattca aggtggtgaa acagcaactt
     4201 caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga actgatagct
     4261 tggacgatca ttcaacaagc aggcgccgat catggaaaga tatttaaaag actttggatc
     4321 aactccttag aaaaagaagc catttatcag tgattccagc aattacgaga cgcagaagag
     4381 acctatccca aatttgaaga agcacaagca cgccagatcg ctgattggtt gatcggcatg
     4441 aacggcagcc cactgtatag tttactcttg cagcaaaaag gcataccagg aagtttttcc
     4501 ttaggcagag tccaaacccc aacgctttac atgatttatc agctgcagga aaagattaga
     4561 aacttccaaa aagaaccata ttttgaagga aaagcccaag taatcgctca aaatggcgca
     4621 tttgatgcga agcttgatcc aaatgaaacc caagcgacac aagaagcatt tgaagcctat
     4681 ttaaaagaaa aaggtgttca attaggaaaa cagccaggta cgatccttca agtcgaaaca
     4741 gagaaaaaaa gtgcggctag cccacgtctt ttttctttat cgagccttca gtctaaaatg
     4801 aatcaactca tgaaagctag tgccaaagat actttggaag ccatgcaagg gttgtatgaa
     4861 ggaaaatatc taagctatcc gcgaacggat accccttata ttacggaagg ggaatatgcg
     4921 tatctgttgg atcacttaga cgaatacaag cattttttaa aagccgaagc gattccgact
     4981 cctattcata caccgaatag tcggtatgtg aataataaga aagtccagga acattacgcg
     5041 atcattccta caaaaacggt tatgacagca gcggcttttg aacaactaag tccactgcag
     5101 caggctattt atgaacaagt gttaaaaaca acggtggcca tgtttgcgga aaagtatacc
     5161 tatgaagaaa caacgatctt gacgcaagtt cagcagcttc agttaaaggc aatcgaaaag
     5221 gtgccgctag atctaggttg gaagaagtta tttggaaaag agagcgacgg aaaagagaag
     5281 gaagaagagc cgctacttcc taaagtgaca aaaggagaaa tggtgacggt ggatctacaa
     5341 gttcttgaaa aagaaaccaa gccaccacaa ccttataccg aaggtacgtt gatcactgca
     5401 atgaaaacag ctgggaaaac cgtggatagt gaagaagcgc aatccatatt gaaagaagtg
     5461 gaaggaatcg gtacagaagc gacacgggcg aatattattg aaaccttgaa acaaaaggaa
     5521 tatatcaagg tagagaaaaa taagctcgtg gtgacgaata aagggatttt gctttgccaa
     5581 gccgtggaaa aagagccgct gcttacgagc gccgaaatga ccgccaaatg ggaaagctac
     5641 ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt ttctagccaa tatccaaaaa
     5701 tttgtctctc acttattgga agtcgtacca gggcaaatcc aatccactga ttttggatcg
     5761 acgttacaag aagtgaaagc agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt
     5821 tgtccaaaat gccaggaaca agaagtctta ctctatcaaa aggtggcagc ttgtacgtct
     5881 gaagcctgtg attttaaaag gtcagaaggg ttcatttgaa gcaacgattg ttttaaaaga
     5941 agacttcact acaggctttg aattttctga aaagaaaaaa acgaacttca agaaaagaac
     6001 acgccgcact gcaaaataat ggagggttaa tcttatgcgt aataccacac acaaacaaca
     6061 agaaatcgaa aatcaaaaag ccaaaattcg ttcagaacag gaaaaaatgc gtgccagcaa
     6121 acgaagactg cagcaattga agaaccagca gaggattcaa aaagaaatcg aattagatcg
     6181 ataaaatctt cctaactatt gtatacgtat aaacttatgt tatactgttt ttataagttt
     6241 atacgtatac aaaggaggga gaaaatgaca aaagcaatgg tatacaagct gctagaagaa
     6301 tttaatggaa ctttggcttt gaaggatgcc aaaaaagcgg gcatttctcc tgtaacgatc
     6361 aaacgtatgg tagatcgagg ggagctggat cgagagtatc caggtttttt caccttacct
     6421 ggtcaatttc ctgatgaatt gtttatggcg caaaaaaaat atgaacgcgg cattatttca
     6481 catattaccg cgttagattt gtatgattta acagatatga tccctagaca gatcgatctg
     6541 actgtacctt acggttatca cgtttctgaa aaaggattga aggaattcgc agttgagcta
     6601 cactatacaa aagcggaatg gtatgaatta ggtaagattg aaataaaaag tcgctatggg
     6661 aatcctatta tagcttatga tccagaaaga actttatgtg acatttggaa tccctggtat
     6721 accgttgagg atgaaatcaa agtaaaagca atcaaaaact atatggagtc ggacagaaaa
     6781 aatcttagaa aattgaatga gtatcgacga attttaccaa cagacaaaac gatgcgttct
     6841 tatattatgg ctttaaatta aaggagaaga tacatgacac ctacgcagtt aaaagcaaaa
     6901 gtgaagaata tttctagaga aaaagaagtt gatccgcagc ttgttttaag acattttatg
     6961 atggaaaaat tcttggaaaa aatctcagag tctccttatc gtgaaaattt tgtgttaaaa
     7021 ggcggcttct tgattggctc aaagtatggc attgaaaatc gcacaaccaa agatattgat
     7081 acgactttac gggaaatgaa agtaaccaaa gaaacattaa caacggttct aaacgaaatt
     7141 ttttcaacgc cgacgaaaga agacattcaa tttgagattc aaggaatgaa agaaacgaga
     7201 gaagccgatt actatccagg atttagctta cgtgtgctgg cacatttgga aaacatgcgg
     7261 ccagatttta aggtagatgt gactacaggt gactcgatct atcctgcaac gatcactcac
     7321 agtcataaat tgatgtttga ggatcgaacg attcctttgg aatcctatcc tacagaacaa
     7381 attattgctg aaaaattaag tgcaaccttt gattttttaa cagataattc gagaggaaaa
     7441 gatttttatg atctctatac tattcctaaa atggagaaga tcgatgagaa gaccttgaaa
     7501 gatagtgtga gaaatacatt tacacgtcga ggaaaaactg atccgttaaa gatatactat
     7561 gaaaaggata tggatgtact gaaaaatagt cacgagctaa aagatacctg gaagaaatat
     7621 caaaaaagca atccttatgc tgcatcgatc acgtacgatg agacaatgga ttcgattagt
     7681 aatttgatgg aaaaagtgat tcacgtcgaa gaaaaagagc aaaaactagc ttatcaaaga
     7741 caccaacaga tgaatagaga aatggagaga taaagatgat taataatgtg acattagtag
     7801 gacgcttaac caaagatcct gatttacgtt ataccgcaag tggtactgca gtagcgactt
     7861 ttaccctagc cgtcaaccga aatttcacga atcaaaacgg caatcgagaa gccgatttta
     7921 ttaactgcgt gatttggcgc aaaccagcag aaacaatggc gacattagct aaaaaaggta
     7981 gcttgattgg tgtagttggg cggattcaaa cgcggactta cgacaaccag caaggacaac
     8041 gtgtatatgt gactgaagtt gtagcagata actttcaact attagaaaat aaagcagcga
     8101 cagagagccg tgcgcacgct gatcaatcgt ccaccagtcc aagcaccgcc acctttgaac
     8161 aacggataca gcgacgccta acaacaatgg attgaacgct tcacagaatc catttggtgg
     8221 acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag aaggagtaag
     8281 catggcaaaa aaaattagtc tgacgatgga tctattgata cccgtaattg catttataga
     8341 cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga aaggatcact
     8401 atttcttgta ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag gaaaggataa
     8461 ttccttttgg cgattgacta aagtcacagt agcagcgaca gctgttatac ttacggctat
     8521 tctaccgctt tatatgtaga gttaacggga aatcgttgaa cttcttgcga aatattgcta
     8581 taatgaacat gaaaaagagg agttgcgcta acaacccctc attaacagca ccgattaaag
     8641 aacggtggca gaatattagt tatttggaaa taaccgctga cctgctaaag tagacggtta
     8701 tttctttttg tcatttttaa gcatatttac aatcaaacta atcaaagcaa tggtaaacat
     8761 accaaaacca agaatggttt gaattgtctc atatgcggac aaaaggctta ctccttttat
     8821 agattttagc acttacattc ataagcacca ccacctttcc agaagtcgcc accgtcttca
     8881 aactttactg tcttgactat tatagcatat ctaaaatcga ataaaaattc tcaattttaa
     8941 ctgtttttgc tataatttta gcgaggtgat cgtgatgaac aaatatgttg gtttgcttga
     9001 taaaatccga gtaataaaaa cacaaccatt attggttcgc tttactttac aaacaattca
     9061 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgt taattatgga
     9121 tgatggaaaa tacaatattg ccgtcacagg tcatttcaat aaacggaatc aattagtcat
     9181 tgaatcgatg caaattagaa accctgatca ttttacgcga tcaatgggca tataaataaa
     9241 agaagagctg caatcctaat gtaggactgc agctcttttt gttttacata gaaacaggaa
     9301 gaaagggaaa ggcctgttta ggaaggcaca agtgccttct caattgaaaa acctttctct
     9361 cactccaacc gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga
     9421 taaggggtct tgctttgtaa gacaaaatag gaaaagcttc ctctcaccaa aaggttcgtt
     9481 cctgttcgct atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa
     9541 aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt
     9601 tgattcattc tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt
     9661 cctaagagga ggcagaatca atcatgcgaa agaaatttga ttatttgggt gttcccttca
     9721 gcgaattatt cccaaactat catgcaccac atactgtcga atgcgattgt ggagaaagag
     9781 cgaaatgtat caaaagctat tgtttgtatc aatgtccaac atgcgggaaa aaatatacct
     9841 tgtcttatgg cgattatatt ttgattgagg agaaaggaaa gaaacgctga tgaaaacaca
     9901 acacaaatta ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc
     9961 tgaaaaagta gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt
    10021 agaggatgca ccgactatgc tatttgaaga cctagtgaag attgcaggat gtgaactgca
    10081 ttatcaagaa aacttggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta
    10141 aaaaggagaa aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga
    10201 taaaatggca caaaaaatgg aggatatgac gttcctatac aacgaaacac gtgttccaaa
    10261 aaaacattat aaagaaaaat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga
    10321 aatgaatctt attgcgacgt actatcgaac actggaagaa ctgaaaaagc aaaatgcaaa
    10381 atggtttttc caagccttac tatgctttga aataggtgtc aaaccaagca ctattaaacc
    10441 ttctgaatac caggctttag aactaacgta tgcgaaattt atcgaaacga aaaaagccaa
    10501 gactgtttca agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcatatta
    10561 caccatgaaa aaggaagaca acgaaaatga ataaggtgat ttatcaacta acaacggatt
    10621 ccatatcaac gatttgaaag aaaaaaaggt caataatcat ttataccgaa aagtgagaat
    10681 gtgtggaata aggacaaaaa aagcctagta actaagggaa tggttcttat ttcgcaccaa
    10741 ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa gcccgataac ttaagagaaa
    10801 gaaggtgatc catttgaaaa agattattcc cttattgtta gtactgtttg ttggcgaaat
    10861 acttatgcga gatagtgcag ctgtacagtt aaacctcgct gtatttttgg taatactggt
    10921 gttttgtttg atcttttggc ggtttgtatt agtcctcgtg agtctactat tcaagaagat
    10981 tgcagccata tttttcatgg ggttatttcg atgaaaggag agagtaccaa tgccaattgc
    11041 aataggaaat aaacggttac ccgtgacatt agatgaaaag agacaaaaag aattgcagca
    11101 actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11161 gattgccaag aaaaagcagg atttgaggta ccagcactaa gaaagtgacg tcacttttta
    11221 tactaaaacc taaaagtgat atcactttta gacaatggaa aaggtggaaa taagatgaag
    11281 aaaaaacaaa ctaaactatc gttagcagat attttaacac agttaaccgc aactgaagat
    11341 ggtgttgtag aatttgaacg atctgaaatt tcggtagtag atgatcgtta ctttaaaatg
    11401 ccttactttt ttgaccaagc aaaggtcatt tgcctttgtg gctacgatgg ggtacgtgat
    11461 tactttggaa ttagaattac tgaagaaaaa gtcgtttggg taaacaatca tacagaatta
    11521 ggtgcgctag cctttgaagg aactgtattg gacaatatct ctattgtatt cgaggaagaa
    11581 agctttacgc ttgaatgcga taagttaacc cgatatatcg atccaaaatt ctatgaggat
    11641 aaaaacctgg catgggaact ggctttatag acagagtgct agtgcaagta aaggaagaaa
    11701 ggaagatcac catgaaatac aatttattag accataagcg aatgtgggta gagaaattag
    11761 aggaagactt agaacagttg gaattgttag gtcataataa gacttcaacg gtgtataaag
    11821 aaggactaaa acgaacgaaa aaagcacaaa aagaactagc aacactggaa agtagggttc
    11881 agaaaaaaga agtagactta acacacactt acctttagga gtagaaaatg gcagacaaga
    11941 gaaaagaaaa gattcaaaaa ttactggcgc ttgcggcgga tgtaaatgat gaggaatcag
    12001 tgagcgcatt agccaaagcc cagcaattga tgatggacta tgatcttact gaagatgaga
    12061 ttttcaatta tcatgagcag caacggatag aagcagtaga aacgcacgtg atctatcgtg
    12121 gacgtccgca aaaatggctg tatcgtttat ttagaaatta agataaaaaa ggctagttaa
    12181 caggtttgtt gactggcctt ttttgatatg tttgtacatg ttttattgtg ttttaatttt
    12241 tctctttttt atagcaagct ccttttataa taaatattcc gaaaagtgaa gaccattttt
    12301 tatttattaa gactattatt attagtgaga taaaatttga ttgatcttac agaatattgg
    12361 tgacgattat gacactaaag atactattta tgaaaatttt gcaaaaatgt tctgaaaatt
    12421 acatataatt gttttataaa aaagggaggc aattatatga agaaaaaatt tgttagtatt
    12481 ttttgatttt aggaattgtt ttattgagtg tatctacttt aggaattaca gtagatgctg
    12541 caacttatta tggaaatggt gtttattgta atactcaaaa atgttgggta gattggagta
    12601 gagctcgctc tgagattgtt gatagaggag taaaagctta cgttaatggt tttacaaaag
    12661 tgctaggtgg agtcggtgga agataaaaaa gaaaaacttt ttgacttaat tgcgaatgca
    12721 tataattcaa agtcatataa taatgtagaa gtaaaagaag gatttcttca agatgctaag
    12781 aaaatacgag aggataaacc atatcctgaa attattatat cagtgtacaa acatttatct
    12841 aaattaaaag ctgaggaac
//
LOCUS       DACETN010000077        12674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DACETN010000077 DACETN010000000
VERSION     DACETN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.079"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="IXF98_002027"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="IXF98_002027"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1556333.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGKMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="IXF98_002028"
     CDS             1371..2546
                     /locus_tag="IXF98_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1556334.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="IXF98_002029"
     CDS             2550..3191
                     /locus_tag="IXF98_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556335.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="IXF98_002030"
     CDS             3188..4105
                     /locus_tag="IXF98_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1556336.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="IXF98_002031"
     CDS             4102..4773
                     /locus_tag="IXF98_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556337.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="IXF98_002032"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="IXF98_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="IXF98_002033"
     CDS             complement(6004..6270)
                     /locus_tag="IXF98_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1556338.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="IXF98_002034"
     CDS             complement(6270..6533)
                     /locus_tag="IXF98_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1556339.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="IXF98_002035"
     CDS             7008..7193
                     /locus_tag="IXF98_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1556340.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="IXF98_002036"
     CDS             complement(7335..9158)
                     /locus_tag="IXF98_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1556341.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="IXF98_002037"
     CDS             complement(9755..12460)
                     /locus_tag="IXF98_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1556342.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2636 c         2389 g         3998 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttttcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atct
//
LOCUS       DACETN010000078        12672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DACETN010000078 DACETN010000000
VERSION     DACETN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.080"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..157
                     /locus_tag="IXF98_002038"
     CDS             <1..157
                     /locus_tag="IXF98_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1556343.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
     gene            692..1264
                     /locus_tag="IXF98_002039"
     CDS             692..1264
                     /locus_tag="IXF98_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1556344.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDAFDEDED"
     gene            1539..2156
                     /locus_tag="IXF98_002040"
     CDS             1539..2156
                     /locus_tag="IXF98_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1556345.1"
                     /translation="MDRIAGKAHTKHYQKGEFLFRSQEKDDALYIVNRGKVRIYRLSN
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTSVCMIQQHDVQEFLKEYHAI
                     SLKLLGEMSQRLESSGRQTAQVAVESVITRLVLFLAENVGPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2308..3162
                     /locus_tag="IXF98_002041"
     CDS             2308..3162
                     /locus_tag="IXF98_002041"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1556346.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAAPQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILTIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWVNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIIVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3309..3542
                     /locus_tag="IXF98_002042"
     CDS             3309..3542
                     /locus_tag="IXF98_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1556347.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3740..5644
                     /locus_tag="IXF98_002043"
     CDS             3740..5644
                     /locus_tag="IXF98_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1556348.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAVPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDIKRFEQHGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5954..6316
                     /locus_tag="IXF98_002044"
     CDS             5954..6316
                     /locus_tag="IXF98_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556349.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6333..6944
                     /locus_tag="IXF98_002045"
     CDS             6333..6944
                     /locus_tag="IXF98_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1556350.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(7046..7954)
                     /locus_tag="IXF98_002046"
     CDS             complement(7046..7954)
                     /locus_tag="IXF98_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1556351.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7996..8310)
                     /locus_tag="IXF98_002047"
     CDS             complement(7996..8310)
                     /locus_tag="IXF98_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1556352.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8609..10540
                     /locus_tag="IXF98_002048"
     CDS             8609..10540
                     /locus_tag="IXF98_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1556353.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10833..11384
                     /locus_tag="IXF98_002049"
     CDS             10833..11384
                     /locus_tag="IXF98_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1556354.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11697..12239
                     /locus_tag="IXF98_002050"
     CDS             11697..12239
                     /locus_tag="IXF98_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556355.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         4112 a         2131 c         2634 g         3795 t
ORIGIN      
        1 ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt ttcttagtct catcttttga
       61 tacttacaac caaaaattcc taggtcgcag tcataaaggc ttccaacagg ccgaaggcga
      121 gctcgaacaa atgctgagtc aaccgatgga gaattagaaa caatctacag tagggaagaa
      181 ccatttacac aaaattattg acgctcccaa gctaaaactc ctattaatcg atctactaaa
      241 aataaataaa aaaattcttt tcaattttta tttatggtgt gacataaata aagtaaagtt
      301 attcttctag gttaatctta ttaatgctct ttctcactag tgttgcatgc aacactagtg
      361 agaaagagta ttaaatatac tgcaaggtaa gacatttggt aattgaagaa taacaagcaa
      421 aaaaatagca gtcaggtctc ttgacaaagt atagttgcta ttctttttta ggccctactt
      481 taatgaaatt tttgtttgga gggaacgact tcattccttt gattatttca gttgaattta
      541 atagagaagc ctgtcgtcta ttaagaaata cttttcttga tgagcgtcaa tttttttaac
      601 ggaaatctat cctatactaa attcagtaaa gaaaaaggag gaatcaaaga aagctatttt
      661 aaacaaacca aaattatagg aggaataaat catgactgaa aataaagcta ctcaagaaag
      721 aacaccccaa gaaagattgc aagaagaaca agaacacaag gaacacgttc atcatacgaa
      781 aattaatgca gcggccattg cggatcatct tttaggcaac atgcatacat tacatgtgaa
      841 actgcaccaa tatcactggt atgtaaaagg agccaatttc tttacgttgc atgaaaaatt
      901 tgaagagttg tataacgaaa atgaaaaatg gttcgacaaa cttgcggagc gtctaattac
      961 ttctgggcat aaacctgctt cgacaacggt tgaatttgaa aaatattcga tgctttcgga
     1021 agatgcagtt aataaatacg ctaaagcaga agacatggtt gaaaatatta tcgaggactt
     1081 cagaagcact cgtgacctaa ctattcgcgc gattcgttta gctcaagacg agggtgacga
     1141 tgctttagaa gatacattga ttgcttttaa aaatgactta gacaagaaca tttggatgct
     1201 gcaagcgttc attggcaaag aggcattgga agacgacgat gcgtttgatg aggatgaaga
     1261 ttaggttttt cgtaaacata catttaaaaa ttaaagattt tggggttggg actctttcca
     1321 gctcccattt ttttgccgtt aaaaagactt ttcttgatga ttgtcaattt ttttgaggta
     1381 gggggccttt atactaaata taggaaagtt aaaagaagga caataaactg ataaagggga
     1441 agaacgaatt gactcaatac acccaaccac atgcagcagg agaccactca gcttgtatcc
     1501 gcttagtccc gatattcaat catttggagg aaagctcgat ggatcgcatc gctggaaaag
     1561 ctcacacgaa gcactatcaa aaaggggagt ttctgtttcg ctcgcaagaa aaagatgatg
     1621 ccttgtatat tgtgaatcgc ggaaaagtcc gcatctatcg cttgtctaat tctggcaaag
     1681 agcaactagt tcgtattttg aatccgggtg actttacggg agaatggacg ttgttcaatc
     1741 ctgatgctgt gcacgaggaa tacgcagaag ctactcgaga tacgtctgtc tgcatgattc
     1801 aacaacatga tgtacaagag tttctaaaag agtatcatgc aatttcgtta aagctgctag
     1861 gagagatgtc acagcggtta gaaagctccg gacgtcaaac tgcacaagta gccgtggaga
     1921 gtgtcattac ccgtttagtt ttgtttttgg cagaaaatgt gggacctgaa atgggtaact
     1981 ctcccaccat caccctgccg atggcaaaaa aggacattgc ttcctattta ggaacgacac
     2041 ctgagaccat cagtcgcaaa tttggagaat tggaggacga gggattgatt caacagctgt
     2101 ctgggaaaag gatcaagatt caggatttag atgatttatt gctttacagc gaataatcgg
     2161 acacggaaaa gtggcgtatt acgaagcgag tgaccaggga gctaaaagtg aattcactgt
     2221 ttggtttccc tggaggttag ccggaatctt gttcagcatc accatgacta gaatgacgcg
     2281 aaaaattgaa gaaatggagc aaatagaatg ggattcgtat taggcgtttt gaatccccat
     2341 gtggtggaga ttggttctgt tgtgattcat tggtatggag tcattattgc agcgggcata
     2401 ttagcggctc cccagttgag caccaaggaa gcaaagaaaa aaggactgga agaggatacc
     2461 attatcgata tggccttatg ggcaattccg attggactcc tcggcgctcg gctttattat
     2521 gtgttatttg aacttggtta ttatctgcaa aatccaggac aaatccttac gatttggaat
     2581 ggcggtattg cgatttatgg gggattgatt gcaggagggg gtacgctata ttggtatgca
     2641 aagaaaaaag gtacttcttt ggcactcgtt cttgatattt tagcgcccaa tgtattgtta
     2701 gcccagtcca ttggtcgttg ggtcaatttt atgaatcagg aagctcatgg tgggccggtt
     2761 actcgtcaat tcttggaaaa tctttattta cctgaattta tcattgaaca aatgaatatg
     2821 aatggtacct actaccatcc gactttttta tacgagtcat tgtggagttt gttgggcttt
     2881 gtcctcatcg tcactatacg gaaccgaaaa cagcttttgc gtcaaggaga agtcgctttg
     2941 agttacgttt tgtggtactc agtgggtcgg ttcttcattg aaggtatgcg aacggacagt
     3001 ttatggattg gcgagtggat ccgcgtttcg caagccttgt ctctggccct gtttattggc
     3061 gcgattatag tatggtttat acgcagacaa gattatccac caatttctta ttattctgat
     3121 ggcaataagg ggcagaaaaa gactattaag atggtgaatt gagcaaaaag ataaaaagaa
     3181 gtgcaaaact tgatatgcgt caattttcgt ttgctaaaag ggagctatac tacagttgta
     3241 aagaggaaaa ggcagtgttc attaatctgc tgccgataaa ataaaaacac ataaaggaga
     3301 taggaattat ggaaaaagct atattgcaat tagaaacgtt gtcttgtcca agttgtatgc
     3361 aaaaaattga aggtgccgta aaatcagtta acggtgttga taaagacagc attaaagtat
     3421 tattcaactc cagcaaagtc aaagccaatt ttgattcagc tgtcacttcg attgaagaga
     3481 ttcaaaaagc tatcgaaaac gtaggctatc cggtccttaa agcaaaagtc aaggccgctt
     3541 aacagattaa atgaactagg caattctgga agaaaagcta tttttccagg attgcttttt
     3601 ttgttgcaat ccgattcgag gtgcttagag acgataaaag agaactttct aaacttgatg
     3661 tcagtcaatt aattacttag gcaattatct tatactgtgt ttatcaataa aagagaaatt
     3721 aaatttggag ggaaaaagca tgcaacaata tatattaagc aagaaaaatg ttatcaccgt
     3781 catcagtgga ttgttaatcg tactcggttt cttcagtcac tttgttttag aaaacgtagg
     3841 actttcagag tggtctttga tcatcgcctc tgtctttggg attacgccaa ttgccattca
     3901 agcgtttcaa gcaatgaaag tcaaagtcat cagtattgat gtcttagtca gtattgcagc
     3961 gattggtgcg ctttttattc aaaattatga agaatcggct attgtcacat tcttattctt
     4021 attcggacac tacttggaac aacgaacatt gaaccaaacg cgatctgcta tcaaagaatt
     4081 gactgaaatg gcacccgaaa gcgccttgaa acaaatggat aatggcgaat ttgaagaagt
     4141 agaagtggat gatatagatg aaggggatat cttgctcgtt aaaacgggtg cgaaagttcc
     4201 agtagatggc atagtcctta cgggtgaagg gcatatcaat gaagctagca ttacaggtga
     4261 agctgttcca gtcaacaagc tagctgatgc agaagttttt gcaggaacca ttttagaaaa
     4321 cggaacgatt caaatcagag ctgaccgagt tggtgaggac accacatttg gaaaaattat
     4381 tgaactcgtg gaagaagcac aagattctaa atccgaagcg gaacggttca ttgatcgctt
     4441 ttctaaatac tacacaccag ctgtcttggt tctggcaatt gttgtatggg cgttcagtca
     4501 aaatattgag ttagcaatca ccatcttggt tctaggttgc ccaggcgcat tagttatcgg
     4561 agtgcctgtc tcaaacgttg ccggtattgg gaatggtgct cgtaacggtg ttcttttaaa
     4621 agggagtgaa gtcattcaag acttcagcaa tgtggataca attgtatttg ataagacagg
     4681 tactttaact gtcggaaacc caaccgttgc agcgactgaa ctgtatgaaa aagatcctgc
     4741 tgaaacgctt ggctatctgg ccagtgtgga acgggaatca gatcatccat tagcaaaagc
     4801 ggtcttaaat caaattggag aaacaaactt ttatcctgtt gaaggaactg aagtcgtgaa
     4861 aggcggcgga atcgtttcaa gtgtagctgg acatagagtg gctgttggga atgtggcctt
     4921 gatggaaaaa gaaaatgtca ctctcagcaa aaaggtgcaa aaagatatca aacggtttga
     4981 acaacatggg aactctctcg ttttgacagc ggtcgacggt gaattaaaag tactgatggg
     5041 cattcgcgat caagtccgtc cgggtgtgaa agctaatttg caggaattaa aagacttggg
     5101 cgtgaaaaat ctagtcgttc tttcaggaga taaccaagga accgttgatg tggtcgccgg
     5161 cgaacttggt ttgaccgaag ctcacggcca catgttgccg gaagacaaat cggcttatat
     5221 cggaaaactt caacaacgtg gtcaaattgt agcctttgtt ggagatggcg ttaacgatag
     5281 tccgtcctta gctttagcag acattggaat cgcaatggga agcggaacgg acgtagcgat
     5341 tgaaacatcc gatgtcgttt taatgaactc gaacttcagc aacttgcctc acgcattagg
     5401 attagtaaaa gccaccgcaa ataatatgaa acaaaatatc gtgatttcaa ttggagtagt
     5461 gttggtcttg ttgaccagcg tcttcttcag cgaatggatg aatatgtcta tcggaatgtt
     5521 ggttcatgaa ggtagtatct tggtggtaat tttcaatggc atgagattaa tgaagtataa
     5581 gttgaaaggt cgaaaagaac aaaaagaagt atcagcacct gcagagaaag taaaaatatc
     5641 ttaagaataa attagaagag gctgagacaa tagtccatcc ttgaaatgac aaaagcggaa
     5701 actcagattt ttaaatctgg gattttccgc ttttttgagt tcttgaaaaa aaataagagt
     5761 atagagtatg ttctttataa aattaatttt cgtgtaaatg attaaaaaat ttaataataa
     5821 aatttaaatt cctattaagt tgtaacacat gaaattgctt ataatagtgt attgaaatta
     5881 aggtagattg acaaacgagt gatcaaatag atatactacc ttcaaacgac cgtttgaatg
     5941 tgaggtgaga tgaatggaaa aagtttgcca agtgactatc gtacataaag aaaaagtaaa
     6001 tgtaataaaa aaaaaattgg atcaaaaaga tttttcaaac ttacttgtgt tagggaaatg
     6061 tttcagcgat tcttctagga ttaaaatttt ctatgcttta gaaacctata aggaaatgtg
     6121 tgtctgtgat ttagcagaaa tacttactgc tagtgtcgct acaacgtcac accatttacg
     6181 ttttttaaaa aaacatggaa tggcaaaatc acgtcaagat ggaaaagtag tttattattc
     6241 tttagcgaat gaagacatcc tttccgctat tcgtgttttt ttaaagttat cagaaaatct
     6301 atctatgaaa tcttagtttg gaggaagtta ttttgttaca aagtattttt tcagctatcg
     6361 ctgtttatat ttctaccagt attgattatt tgtttatctt gctgattatc ttttctcaaa
     6421 gccacacaaa aaaaggtatt cgtcacattt tttggggtca atatcttgga acaggtattt
     6481 tggtagccgt aagtttgttt gctgcttatg tgctaaattt tattccgcaa gattggatca
     6541 ttggtcttct cggtctaatt ccgattttct taggaatcag agtcgctttt gttggcgaag
     6601 aggaggaaga agaagaggaa gtcgttgaaa aactcgaatc tagaggaact aatcgcttat
     6661 tctggacagt tgccttaata acgattgctt ctggaggcga taatttaggt atttatattc
     6721 catactttac ctcgttagct ttttcagaaa tcattattgc tttaatcgta ttcgctatct
     6781 ctgttgcggt tctctgctat atcagttaca aattagccaa gatttctttt gtttccgaaa
     6841 cgttagaaaa atacgaacga atcattgtcc ctataatgtt tattgcgtta ggaattttta
     6901 ttatgataga gaacgggacg atacaaacgc tactaggttt ttaaaaatca taatcacgaa
     6961 agataaaaag agaggcagcg actaatcaaa atcgctgcct tttttgcttt tcaaataatg
     7021 aagtattttt tagcgtagaa ctatcttacg tgttgtggga gtatcccttt tcaagctctt
     7081 ctttgcttgt agttatttct attgtgatat gattctctac gcttgatttg tgtgcgactt
     7141 ctgcaatttc ttcttttatt tccctagcct tgtctatgtc tgaagggtct ataagaaccg
     7201 tgatgataag ggcgttttct tccccgtcca gtgaccaaat gtgaaaatgt gagaggtctt
     7261 ttacagcagg aacattttta atggagtccg ccaagctctt tacgtttaca ttttcaggcg
     7321 aaccattcaa taaaattttc attgtggtga aaaattcagg aacggttttg tagaggatat
     7381 aaagtgcaat caaaatcgat aagataggat ccagacgata tgcctcggta aagttcatta
     7441 gaatactcac aatcaagact ccaatccacc ctaatacgtc ttccagcata tggaggttca
     7501 acatgctctc gtttttggat gtgcctttgc ttaaaatcca cgcagcatat ccattcaatg
     7561 caatagctac gagagaaagc cagaacatgc cactataatt tacgggttgt gggtcaatca
     7621 aacgtggcac actacgatag atcataaaaa acgagccact aattagcaca acgccagtta
     7681 tcagcgcgcc cagtaaagaa aagcggttat agccaaaagt gaaccgttca tcttgttttt
     7741 tctctgaata accttgaaaa aaccaagcga atccaattga aatagagtcc cctaaatcat
     7801 gaactgcgtc ggacataatg gacacactat tgaaaagaaa gccaaagaaa aattcgctta
     7861 tagagaaaat aagatttatg aagaaagcaa ttttaatatt ctttttcggt tgattctgat
     7921 tcgagtgatt atggtcttta tctgattttg acatttaaaa acctcctaag tttgtatgct
     7981 acatactaat tggttttatt tttctaattc attaacatga gttaagactt gttcgagaat
     8041 actaaaaaca tgaaggtcat ctaaactgta aagcatactt tttccttctc ttcttgattt
     8101 cactaagcgt gcgtctttca acgtttttaa ttgatgagaa atagcggact gttccatgtc
     8161 cagagaaata gcgatatttc caacactaca ctcttttttc tgtaaaagaa agagaaccga
     8221 caatcgtgta ggatcactaa tcaccttaaa tagtttgctt attgacagaa tagatccttt
     8281 atcgattgga gaagttgttt ttatatccat agtgttttta ttcctttcat atgaccttcc
     8341 gttcatatct atattaccat atgagcgaaa caatgctaat cttttgttta taaaagagaa
     8401 gtggcgttct ctttatcttg acaaaatgta tatagcgata taaaatgagt atacaaacat
     8461 atgattaatt agtcatatgt aaggtggatt cattacgatt tacttttgga ataaacgaaa
     8521 ggagtcagaa atagcagaaa tttatgtgat tcaagaagta aatgatggaa aacaaaggaa
     8581 ttataaatta tatagggagg caatagaagt ggaaactatc caagaacaac agtctcaaga
     8641 aaagcataat cataatcaca accacgatca cgaccatgat catgggaaaa tgccagtcgt
     8701 tttgtacttt gttggtttag cattagcagt gattgctcta tttttgaatg aagattatca
     8761 gttggtacaa aatgttttgt tctcaatcgc tacaatcagt gccgggtatc acgttattct
     8821 tcttgaaggc cttggagaaa caattgagaa ttcaaaagca caaaaaaagt tcatacctaa
     8881 ttctcatatt ttaatgggat tagcggcaat tggagcttct ttgatgggga atttttggga
     8941 aggaacatta ttgattctta ttttttctgg cgcacatttt ttggaagatt atgcagaagg
     9001 aagaagcaaa cgagaaataa cgaaattact cgaaatgaat ccaacgactg cccggttaat
     9061 cctgcctgat ggtagtacaa aaaatgtgga tgtcagtgaa ttaaaagtgg gagatcaact
     9121 tcaagtgttg aatggcgatc aagtacctat tgatggcgtc attttgtctg gatctacatc
     9181 cattgatgag tcgtctatta ctggagaaag tatcccgaaa gagaaatcca aaggagatgg
     9241 cgtcttcggg agtacaatca atggaacagg taccttcacg atgaaagtca ccaaagaaaa
     9301 caaagataca gtattttcaa aaattttaca attagtgaat cagaaccaag ataaccaaac
     9361 aaaagctgcg agtattatcc aaaaatttga accgaaatat gtcacagttg ttttaattgc
     9421 cattcctcta ttcatgttat tggctccttt tctacttgat tggacatggt cacaaagtgt
     9481 ctatagagga ttggtccttt tagttgcagc ttcaccgtgt gctttagcag cagctactgt
     9541 ctcggcaacg ctatcgacga cttctaattt agccaaaaaa ggagtccttt caaaaggtag
     9601 ttcttacctg tctcaattag ccgatattaa agcaattgca tttgataaga cgggcacctt
     9661 gaccaaagga aaacctgaag taaccaatta ttattttgct gattccgtga acgaagaaaa
     9721 catgattgat atcgtagtag ctcttgaaaa agaatccaat caccccttag cagatgctat
     9781 tctaagaaag tttgagcaaa aaaacaaact aactattgaa gtagaaaacc aaattggtaa
     9841 aggattgacg ggagattaca aagggagaaa ttatcgtata ggaaaaccga cttcgtttga
     9901 tgatgtttca gatgaatata gtcgtttaaa taatgaatgg gcgtcagaag gtaagacagt
     9961 cgtatacgta gcagtagatg aagaggttat aggccttatt gcccttatgg acgttccgag
    10021 tgaatatgct aaagaaacca ttgaatattt taggaaacaa ggtatacaca ccacattaat
    10081 tactggtgac tcagaaatga cgggaaaagc ggttgctaaa caattaggaa tagatgaagt
    10141 gattgcgaat gtcatgccgg aagacaaatc cagaattata gacgaacaaa aagaaaaata
    10201 cggcgtgacc gccatggttg gagacggtgt aaatgatgct cctgctcttg ttaatgcaga
    10261 tgtgggaatc gctatgggag atgggactga tgtggcagta gaggtatctg atttagtttt
    10321 aatgcaaaac aatttatcta aattggtaca atctcataat atttcttcga aaatgaaccg
    10381 tgttatttgg cagaatatta ttttttcaat ggccgttgtt gcttttttaa ttgtcgttag
    10441 tttcttaggc ttaacggata ttgcaatcag tgtcattatt catgaaggaa gtactttggt
    10501 tgttattttg aatggacttc gattgctaaa ggcagtctaa agaatgattg cacaacttca
    10561 aattaatcga gtaatagaga taattaagga ttacactaca ttttttatct ctattacttg
    10621 tttatattta tctagatgtt caaagtcaaa atgatatatc aaccatactc ttgtggcgcg
    10681 ggttgaatat aagatacatg aaattggata aaatcatgta tcaagaatcc tatccttata
    10741 gggttctttt tttttactcc ccctaaacac acgatttcgt gttgtaataa ctataatcat
    10801 gtattcgaac agactaaaaa ggagggattg tcatgggcta taatgttcag ccactacggt
    10861 cgcaacagga gattaatgat tttttatttt gcctgcgacg gaataaaaat gcggaacgtg
    10921 acgttttctt gtttctaatt ggaattaaca gcggtctgcg catgtccgac atcgttaaac
    10981 tcaagaaaaa agacgtgatt acctcaaaaa acccgcgcat cgtggagcaa aagacaggga
    11041 aaacacgcat tttatactta agcagcttac aggacttgat tcacggctat accgcaccct
    11101 tagatccgga ggcttacttg tttcccagta ctaagggcgg ccatttagaa gtcaatacgg
    11161 tctaccagat gtttcaaaag gtcgcgaagc tgttaggaag ggacgatatc ggcacccaca
    11221 ccctacgaaa aacctttggt taccactatt acaagaaaac caaagacgtg gccaccttaa
    11281 tggaaatctt tggtcacagc agcgagaaga ttaccaaacg ctacatcgga attaatgaag
    11341 atgaaatcag tgaaacgtta ttaaattttc ggttaggttt ttgattgtat tatataatag
    11401 ataagcacag taagacggtt ataatcaccc acagtattgg acaaatcaac ctttttttgg
    11461 tatattatat atacaaatga gagcaggctc atagtcaact tgatataaat ccggaacata
    11521 agggtccggc gggtggcgag caccaaaact aaactcgata caaatccgga acgtaagggt
    11581 ccggcgggtg gcaagcacca aaactaaacc tgtacaaaag gctgctcttt tgagcagctt
    11641 tttttattgg gaaaaaggag cattcttcgc acgcggcaaa aaaagaatac aaacgcatgt
    11701 ctataaaaga gacaacccaa attaccagac agttaaacgc gatttataag gctgctcatc
    11761 tcttacaaga acattttgta gataaaaaag tatcctttgt cggagaagtc tcaacggtag
    11821 cgattatttt ttcgacaact aattttatgc acctctgtgg cattgattat cgcagaggca
    11881 cacacttgtt ttttcaagat gccttagaca ggaaaataaa tttacaggat attcaaatca
    11941 aaacggatgg gacgaccttt cagaaattac aagtaatagg tagccttgat ttattattgg
    12001 ggaagcatat ttctattgtt ggcagaggtg tttactcttc tcttcgctat gatgcagcta
    12061 ttcgaacaag gaaaaaaata ttggctctgt ctttgaaaca aaacggatta atctatatcc
    12121 ctatctcttt gttaaaccta tcatcaaagg aaattggacc tggacaaaaa gttactggca
    12181 tttttagcga ggatttaaca tccggggaac ttaaaatgat tatggaagtc atcgattaat
    12241 cattaatagt gatatgcgac gtcattttga agtaattatt ctattttggg ttataaaata
    12301 aagcatttta agacgatttc aaatcattca tgtgggttat ttatcacgga tgagcttgaa
    12361 atcgtctttt atacgtatca aattgaaatg ttcggcatac aacctatcgt tgttactaca
    12421 ttactaattg tcacttgtta aggaacttgg atcctttcta aacattccct agagataaat
    12481 tttactttat ccctccgtta atatccaatt taagcacctt tccgattcag ggttaatttg
    12541 catatcaagt gcagcttgtg ttctagtaag attagccctt ttagtccggc tgagttgact
    12601 tatggatata agatatggtc atagcaaata gatactacgt atagtctgtt aatttagtgt
    12661 cattaatctt aa
//
LOCUS       DACETN010000079        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DACETN010000079 DACETN010000000
VERSION     DACETN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.081"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            6..305
                     /locus_tag="IXF98_002051"
     CDS             6..305
                     /locus_tag="IXF98_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1556356.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXF98_002052"
     CDS             378..1739
                     /locus_tag="IXF98_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1556357.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXF98_002053"
     CDS             1761..2021
                     /locus_tag="IXF98_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556358.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXF98_002054"
     CDS             2035..2787
                     /locus_tag="IXF98_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1556359.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXF98_002055"
     CDS             complement(2881..3486)
                     /locus_tag="IXF98_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1556360.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXF98_002056"
     CDS             complement(3539..4513)
                     /locus_tag="IXF98_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1556361.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXF98_002057"
     CDS             complement(4891..5670)
                     /locus_tag="IXF98_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1556362.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXF98_002058"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXF98_002058"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1556363.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXF98_002059"
     CDS             7161..8558
                     /locus_tag="IXF98_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1556364.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXF98_002060"
     CDS             8639..9079
                     /locus_tag="IXF98_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1556365.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXF98_002061"
     CDS             9227..9553
                     /locus_tag="IXF98_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1556366.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXF98_002062"
     CDS             9554..10303
                     /locus_tag="IXF98_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556367.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXF98_002063"
     CDS             complement(10383..10838)
                     /locus_tag="IXF98_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1556368.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXF98_002064"
     CDS             complement(11265..11540)
                     /locus_tag="IXF98_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556369.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXF98_002065"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXF98_002065"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1556370.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACETN010000080        12267 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DACETN010000080 DACETN010000000
VERSION     DACETN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.082"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IXF98_002066"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IXF98_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1556371.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IXF98_002067"
     CDS             complement(740..1462)
                     /locus_tag="IXF98_002067"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1556372.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IXF98_002068"
     CDS             complement(1598..2479)
                     /locus_tag="IXF98_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1556373.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IXF98_002069"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IXF98_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1556374.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IXF98_002070"
     CDS             3719..4198
                     /locus_tag="IXF98_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1556375.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IXF98_002071"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IXF98_002071"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1556376.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IXF98_002072"
     CDS             5499..5627
                     /locus_tag="IXF98_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1556377.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IXF98_002073"
     CDS             5631..6176
                     /locus_tag="IXF98_002073"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1556378.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IXF98_002074"
     CDS             complement(6629..8320)
                     /locus_tag="IXF98_002074"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1556379.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IXF98_002075"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IXF98_002075"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1556380.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IXF98_002076"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IXF98_002076"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1556381.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IXF98_002077"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IXF98_002077"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1556382.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3368 a         2539 c         2207 g         4153 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaa
//
LOCUS       DACETN010000081        11932 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DACETN010000081 DACETN010000000
VERSION     DACETN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.083"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            51..260
                     /locus_tag="IXF98_002078"
     CDS             51..260
                     /locus_tag="IXF98_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556383.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IXF98_002079"
                     /pseudo
     CDS             467..667
                     /locus_tag="IXF98_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1154..1333
                     /locus_tag="IXF98_002080"
     CDS             1154..1333
                     /locus_tag="IXF98_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556384.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1379..1984)
                     /locus_tag="IXF98_002081"
     CDS             complement(1379..1984)
                     /locus_tag="IXF98_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1556385.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IXF98_002082"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IXF98_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IXF98_002083"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IXF98_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IXF98_002084"
     CDS             3019..3990
                     /locus_tag="IXF98_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1556386.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IXF98_002085"
     CDS             4130..5443
                     /locus_tag="IXF98_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1556387.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IXF98_002086"
     CDS             5486..6352
                     /locus_tag="IXF98_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1556388.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IXF98_002087"
     CDS             6349..7197
                     /locus_tag="IXF98_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1556389.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IXF98_002088"
     CDS             7211..8683
                     /locus_tag="IXF98_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1556390.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="IXF98_002089"
     CDS             8700..9572
                     /locus_tag="IXF98_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1556391.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="IXF98_002090"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="IXF98_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..>11932
                     /locus_tag="IXF98_002091"
     CDS             11652..>11932
                     /locus_tag="IXF98_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556392.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2038 c         2402 g         3792 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgcaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat tt
//
LOCUS       DACETN010000082        11906 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DACETN010000082 DACETN010000000
VERSION     DACETN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.084"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(77..283)
                     /locus_tag="IXF98_002092"
     CDS             complement(77..283)
                     /locus_tag="IXF98_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556393.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(299..913)
                     /locus_tag="IXF98_002093"
     CDS             complement(299..913)
                     /locus_tag="IXF98_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1556394.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1060..1743)
                     /locus_tag="IXF98_002094"
     CDS             complement(1060..1743)
                     /locus_tag="IXF98_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1556395.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1852..2310)
                     /locus_tag="IXF98_002095"
     CDS             complement(1852..2310)
                     /locus_tag="IXF98_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1556396.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2394..4157)
                     /locus_tag="IXF98_002096"
     CDS             complement(2394..4157)
                     /locus_tag="IXF98_002096"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1556397.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4378..5838)
                     /locus_tag="IXF98_002097"
     CDS             complement(4378..5838)
                     /locus_tag="IXF98_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556398.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6046..6972)
                     /gene="glsA"
                     /locus_tag="IXF98_002098"
     CDS             complement(6046..6972)
                     /gene="glsA"
                     /locus_tag="IXF98_002098"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1556399.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6995..8422)
                     /locus_tag="IXF98_002099"
     CDS             complement(6995..8422)
                     /locus_tag="IXF98_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1556400.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8453..9667)
                     /locus_tag="IXF98_002100"
     CDS             complement(8453..9667)
                     /locus_tag="IXF98_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1556401.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(10008..10250)
                     /locus_tag="IXF98_002101"
     CDS             complement(10008..10250)
                     /locus_tag="IXF98_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1556402.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10282..10839)
                     /locus_tag="IXF98_002102"
     CDS             complement(10282..10839)
                     /locus_tag="IXF98_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1556403.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10852..11040)
                     /locus_tag="IXF98_002103"
     CDS             complement(10852..11040)
                     /locus_tag="IXF98_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1556404.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11053..11619)
                     /gene="amaP"
                     /locus_tag="IXF98_002104"
     CDS             complement(11053..11619)
                     /gene="amaP"
                     /locus_tag="IXF98_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1556405.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3486 a         2455 c         1945 g         4020 t
ORIGIN      
        1 tgcaccagga ttctttgata atttcttagt tttatgtgtt tttttatatg tttttttcac
       61 ttttcggaat aaatatttat ttgttaaagt aattaaacaa tccgctaatc gtttgatctg
      121 gcatttctat ttccttacca tctaaagtaa cagtagtaac gacaaaatgg tcttgcaatg
      181 gcggaacttc taagtactgc ccttgcagaa aatcattcac tgtttcatat tcttttttca
      241 tttcttttga atctgtcgat ggtttatagc ttatttttaa cataactagt gctccttttt
      301 attcgggacg tgcaatcgtg aaaatttctc ctattgctgc ataatcattt cctgccaaac
      361 ggcttacagc attcaatttt cttggatcga tccttcctgt cttatcatca tagactgttt
      421 catttaaatg atatttctgt acttctagca aaaataaatc agctgttact ttatttttat
      481 tccgtatctc gacatgttca tataaggatg tttcaaaacg aacttttgct tctgtcaatg
      541 ctggtactga aatgattact gaatcagtta aagtcaagtc agtcaggtac agttcgcttt
      601 cctcattagg cagattggct gctgttttat ttacttcttc tacgttgtcc tcatcaagga
      661 tatggataac agcttctctt gtctcaagaa tattctttgc tgtgtctttt actgcatctt
      721 gttttctctg gatcgccaag gataagatgg gtcgattcga actgacgata ttgaagtaac
      781 tgaagggtgc gatattcact atatcttttg ctgatttagt cgttactact gctactggtc
      841 tagggatgac tgaaccaatc agcaacttgt aattttcacg ttctgtcaat tctgaaggac
      901 tccgttcaaa catggtttcg ccttctttct ataaaattcg ttaaaaagca gatcttgaag
      961 acaaaatgaa aagtaatgct tttttcatct tcttagaatc tgctttaaac tatctatcaa
     1021 tttttctttt ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg ctttacttgc
     1081 ggcttcgaca tatcttttag tgatcaattg cggacgggtg ataatagatc caactacgac
     1141 tgaaaaagca cctaattcat atgctttctc tacttgttca ggagagtcag tatgtccttc
     1201 cataattact gggatatcga cttgttctat gatttgattc aataaatcaa aatcaggtgt
     1261 ctgcgtcttt cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa gatcaaaacc
     1321 atcttcttgc gcttgaagcg cttcctcaaa agtcgaaata tctgccatag ctaaccgacc
     1381 gtgttcgtga atcatttgga caagttcagc agttgttttt tctgtcggac gtttacgaga
     1441 agttgcatcc agtgcaatga tttctgcttg cgtctccaaa agttcttcca cttctttttc
     1501 tgtcgcagta ataaacactt ctgacccatc gtaatctctt ttgataatcc ctattaccgg
     1561 taaatcaacg attcgcttga tttcattgat atcttctgtc gtatttgatc gaataccaac
     1621 tgctccgcct tcttttgcag ccaaagccat tctccccata ataaaagaac tgtgtagcgg
     1681 ctcgttttcc aaagcttgac aagaaacgac gactccgcct ttgatttgtt ccagtgtatc
     1741 cacaatatct cctccagtaa tgttatataa aaatacagct tcatataaaa atgtaaccct
     1801 tttctattca ggagtcaata atgagctatt tctttcagca gacctttctt actaaaagaa
     1861 aattacttca aattcttcgt atacgaccag cttctcttct gtaaattcta tgttcaacgc
     1921 ttctttataa gcatgtttaa aaaactcagc atggactttc cgccaataag ctaatgtccg
     1981 atccccttct ccttctttcc aagcatgctc tttcgtgact tcactaaaag gcaagatggt
     2041 cacatttata tttttgatga tacaaactgg ttcatttttt ccattcagga ccacgtctaa
     2101 ttgaccaact tttggtaaag gctcattctc ttgtttatac agatcgtaac cacttgatgt
     2161 accagtcttt atctttttta caactagttc gctcaactcg tctgccattt tttctgtatc
     2221 cccaaatgaa aagaaaccta atttttctga ttgataacta ggatattttt cttggaactt
     2281 tttgacatat tcttctactt gctgatacat aacatccacc cctttgcttt ttataatgaa
     2341 aaaaccatcc agatactagt ataaacaatt atctggatgg tgagcataat gaattattga
     2401 atattcaaga cacgcagact atcttcaacg atttgttttg gtgttaaatg ataacgttca
     2461 taaagctgcc ctacaggaac gctatccgtg aattctttct ctgcaccaaa atttaatacg
     2521 ttcattgaag aatcaccata ataacgactg atattttcgc caaatcctcc tgacaaacta
     2581 ccatcttcta atgtgatgac taattcgtgt gtttctttta atttatctaa tgtttctacg
     2641 tcattttctg tgatcgttcc tggattgatc aacgttgctt cgattccttt ttttctaaaa
     2701 gcttttactg tttcttttcc taattcaaga aaaccgccta aagctaaaat tgctactttt
     2761 cctccattat ttactgtttg ataggctgga atactataat catttgcgat agctgtacca
     2821 tgttctacac catgtgtagg aattcggatt gctacaggct gcgctgtttg ttcgaacgcc
     2881 caatctagca tagagatcat atcctctttt gttgctggag caagatattt gatgttcggg
     2941 atacttgata agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact agctgagatc
     3001 gttccgccac cgactaaaat aatcactgga ttctcgttga tcgctaaatc atgtgataat
     3061 tgatcatatg cccgttgcaa gaaagtactt gattggaaga cgactggacg tactccctga
     3121 gaagccaaag cagaagcgaa agtgatagag tgctgttcag cgatccccac gtcaaaataa
     3181 tgatctggat gtttttcttg gaatgttttc agtccaaagg cgcctggtat cgctgcattg
     3241 actgcggcaa taggagctgt acttgtttgc attttcttat ccaaataatc catgatgatc
     3301 atattaaatg tttctccaga agttacttgc tttgcctccc cggtggacag atcaaatggt
     3361 acatgccaat gatatttcat tttattatcg attgccggct gataaccgcg acctttttcc
     3421 gtatggacgt gaagtacaat cggatgatca atatctttta cttcttcaaa taaacggatc
     3481 accgtttcca gatcgtttcc attatccacg taacgataat caaatcccat tgctttaaat
     3541 aggttattgc tgcttttacc gttggtttga cgaagctcag ctaagttttt atataaactg
     3601 ccatgattct catcaataga catctcatta tcgtttacta atacgattag attggattgt
     3661 aaaacaccac cattgttcaa tccttcaaaa gctaagccac cacttaggga gccgtcaccg
     3721 ataaatgcta ccacatttcc tgattctttc ttcagatctc tcgctttagc taaacctgtt
     3781 gctaagctga tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt actttcatgt
     3841 tggttggaat atccgctcac ttcacggtaa tgtccttttt caaaaccatt ttttcgacca
     3901 gttaaaagct tatgaccata agactgatgg gaaacatccc aaacgatttt atcctttggt
     3961 gaatcaaata cataatgaaa cgcaatcgtt aattctacta cacctagatt cggaccaacg
     4021 tgcccaccta catcgctgtc cttttgcaag attttttggc gcatctcttc tgccaactgt
     4081 ttcatttcct caacagataa ttttttgaca tctgcggggc tattgattgc atccaataca
     4141 tgtaatactg tactcataat acattctctc ctccgtacta tcttctttta atgaaaatag
     4201 atttgttaca ctttatctat tctactactt agagttgact ctaagtcaat agcattttaa
     4261 aaaaatttca gagaaagctt gttatataag tagatcttcg cacgaatcag gactcaaata
     4321 aaaaccttct acagacaatt aaaaagcttt gtagaaggtc tcctaattaa ggtctcatca
     4381 agaaatatgt ttttttaata ggtgaagttc ctgagcaata tgatacaaat ggatgtttgc
     4441 accttttaat gagattggat agatctttgt cttttcagtt tctaatccat tttgatagct
     4501 agcagcatcg cacaaaacca ctcttttttt cgcacataga cattcatcag gcaattcttc
     4561 taatgaacga aataaaatag gatttatttc agcgatgcct tcttccgcat acaaatgaaa
     4621 ttgggttcga taaaaaaatt gtttgactga actttctgaa acaacaaaaa tttctatttc
     4681 ctgtctcccc gcttgttcac tgttgatcaa agaaagactt ttgacgaatt ttcgaatgaa
     4741 attttcattc catcttagtt ggatttgatt tttctttttc cactcttcca atatttctac
     4801 aatttttccg tacagaagtt gttctttttc tgatagaaga tagatccttt ccggtaaaaa
     4861 tacttgtcca tctgcccaca acgtacggac aaattgtaaa aaggattgct tgaaaataaa
     4921 attttctgca tcgtcgattt cagcgatact aataatctgt aaaatcaatt cattcagtga
     4981 taggtcattg agaacaaaat ttttataaac aacttcgaaa tcttttctta acagctccat
     5041 gttctcattg gtataatttc tactgttgaa caaagaaaaa atataataga catcttcttc
     5101 ttgtaagtct agaattcctt tttgctcttc caatccttga cagactaatt gaaataacgg
     5161 cttcgtttct gcgcttttct tatcttcttc tttcagtcca aggggaaatg cctttcttcc
     5221 gattgcgatc tctattcctc gtatgataaa ggacttttca tcttccgaat atcttctttt
     5281 aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa gcttcttcta catacggatt
     5341 tttattttca tagccttccc aattgacata acgtactaaa gctagcaaga tgttccgcga
     5401 gtcaaattct gggataatga tttctttatt tttcaaataa tcattttctt taaaaaaatc
     5461 caatacgatt tttttgattg tatatacttt gctcaaagaa ataaattcaa gatcagccag
     5521 ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa aataagattg
     5581 accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata cgagtttata
     5641 cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg ctagactttc
     5701 tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt gttcgatgtc
     5761 tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata tatttcgctc
     5821 gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat tttatttaat
     5881 catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt caaaaaccaa
     5941 taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc attgtaggta
     6001 gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt caaagatgtc
     6061 agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc tgttaccaaa
     6121 tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag ctgccatcaa
     6181 cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact ctcctgattc
     6241 atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt cttcagtaat
     6301 gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag ctaattttgc
     6361 tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga gtatctccgg
     6421 tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc gattgatatc
     6481 acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag gatcatcaca
     6541 gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt taccggcaat
     6601 caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat gtttgcgatg
     6661 attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg tgttgattgt
     6721 ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg gaacttttga
     6781 catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt gttttgtatc
     6841 caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg ctggaatgta
     6901 atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt gttctaattc
     6961 ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc tggaatttat
     7021 gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa acaatcagaa
     7081 tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca aatgagatac
     7141 aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt acaacttttc
     7201 cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc ttcaaatagc
     7261 ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac atcgctgcaa
     7321 gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag attgttgcaa
     7381 cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct tttgcaaaat
     7441 attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga gaggttaccc
     7501 aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt ttcgcaatcc
     7561 atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc acccctgtat
     7621 taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca aaagtatcta
     7681 aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt ttagggtttt
     7741 ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa ataaaggaaa
     7801 caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc atagacagta
     7861 tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac aagactaatg
     7921 ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg tatttggttc
     7981 cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc aatttcagat
     8041 atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat
     8101 acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac
     8161 cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt tcccatcctt
     8221 tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc aaaccgccta
     8281 ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat tcatataatg
     8341 aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt tttgcaggaa
     8401 ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg tttcaattga
     8461 gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt gtagtagtga
     8521 tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag gcatacacgg
     8581 cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt ggattcgtta
     8641 gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat ccgcccatgc
     8701 catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga aaatcaacag
     8761 cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt gcttctacat
     8821 aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt acgtcgacaa
     8881 aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc gcaaatgcaa
     8941 gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga agtgcaccac
     9001 cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca gctgccataa
     9061 gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt gtgaggatac
     9121 atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg aaatctctaa
     9181 atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg aaataaatca
     9241 gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc cctgcaaaag
     9301 ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga aagaacatta
     9361 aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt ctcatcaaaa
     9421 agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc aatccgaatc
     9481 caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga gacatgatcg
     9541 ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca ggagtcatca
     9601 ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg ataggtaatg
     9661 tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg tagttgtttt
     9721 tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat taataaaagc
     9781 ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa gacaaaaaat aaacggtctt
     9841 ctttttatag aaaaccgttt attttcgtct ttttttattt ttaaagatat tttttcataa
     9901 ggaataaaga atacttcata taaaatatat aaaaaagaac cgcagcgagt gtcgctacgg
     9961 ttctcaaatt tgttcttcat ttcaacataa gtttttcttc aatttgctta taatttacgt
    10021 gcaaagaatg aaacgactaa tactaaaatg attgcaccga tcaatgatgg aatgatagcc
    10081 attcctgcaa ttgttggtcc ccaagttcct agtaaagctt gtccaattgc tgaacctact
    10141 aaaccagcaa tgatgtttgc gatgatcccc attgatgatc ctttattcgt gattgctcct
    10201 gcaattgctc caatgatacc gccgacgatt aatgcccaaa taaatcccat tttaaattcc
    10261 tcctaactga cttcttactt cttattgtac acgtggttcg ttattttctt tgactttttg
    10321 tgcacctttg cctacagcag attttgcttt atctgtttgg cgtgaagcaa attgtcctgt
    10381 ggattttgct gcttctgtta ctttgtcttg tactgtttct tgatcttttt ggtattcttc
    10441 ttgtgttttg atgtctgcaa cattcacatt cacttcgata acatctaaat gtgtcatatt
    10501 gtttacttca gtactgatca attctttgat ttggttgtaa atatcttcga tgtctttacc
    10561 gtattctacg atcacatcta gatccacagc tacttgtttc ttacctactt ctgtattgat
    10621 acctgctgtt acgttatctg tgttgactaa tttttcagcc atatttgaga aaaatccgcc
    10681 atctaatgtc aatagaccat ctacatcttt caatgcataa ccgatgattt tttggatgac
    10741 tttgtcttca taagtcaatt caccattcat catgcggttt tctccttttt catttgccaa
    10801 aactgatcca tttgttgtat tcatgttttt gttttccatc tttgtttcct cctatgattt
    10861 aaaaaattga tcaactattc ctgactgttt caaataaaaa ccgattcctg cgccaattgc
    10921 tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc gttaaccata aaacagctag
    10981 cagtagtcca agacccccaa agataatagg gagcttgtat gctgaaaata gttctttcat
    11041 tatttggccc tcctattcca ctcgagagtg cttgtagtct cttgtatctt cttgttcttc
    11101 ataaccgctg tatgtgactg ccactttgac cgtttctttt gttccgagta ctttagttac
    11161 ttcttcttcg atatcttgca tcaatacacc tgttttccct actaatgaag aagttcgttt
    11221 caagtctccg ttgacatgga tatggattcg atttttcgtt gaacgtatcc gtactttagg
    11281 tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc aacccttcga tcgctttttt
    11341 gtcgattgtt agtttgccat cttcccgttt cataacgaaa gttcctcttg actttggata
    11401 gaaaagaacg actagcaaga tgataatagc aagaacggca aatactacag ctgcccagaa
    11461 aatatattgt tgcatgtagt aattcgttaa agtaaagaag cgaaatcgtt ccaattgttc
    11521 aggcataata tacggcgcat tgatcaacgc aatcgctaga aatactgaaa gtagcagtaa
    11581 actcatgata acaccgatta tttttaaccc cttattcatt ttccacctcc tataaactta
    11641 tttttaccct gtaaataact ctaccgacaa tcacagtata gatagcgaac agttagcata
    11701 gcatcagtaa ataaagcgct accaaacatt gctggatcat aacggcgttt tattgccagt
    11761 ctgatactga aataacaatt gcttacttat tctctattta cttttgattg ctggctatac
    11821 ttaaaggata ttattcaaga ttaaaaattg caaagattat gctcagttat tattacaaga
    11881 ttctatttat ttattaaatt ttttcg
//
LOCUS       DACETN010000083        11346 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DACETN010000083 DACETN010000000
VERSION     DACETN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.086"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..936
                     /locus_tag="IXF98_002105"
     CDS             <1..936
                     /locus_tag="IXF98_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1556406.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IXF98_002106"
     CDS             939..3122
                     /locus_tag="IXF98_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1556407.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IXF98_002107"
     CDS             3119..4120
                     /locus_tag="IXF98_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1556408.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IXF98_002108"
     CDS             4135..5049
                     /locus_tag="IXF98_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1556409.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IXF98_002109"
     CDS             5294..5410
                     /locus_tag="IXF98_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1556410.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IXF98_002110"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IXF98_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1556411.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IXF98_002111"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IXF98_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1556412.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IXF98_002112"
     CDS             9380..10642
                     /locus_tag="IXF98_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1556413.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11346
                     /locus_tag="IXF98_002113"
     CDS             10887..>11346
                     /locus_tag="IXF98_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1556414.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3740 a         1897 c         2376 g         3333 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaaa
//
LOCUS       DACETN010000084        11268 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DACETN010000084 DACETN010000000
VERSION     DACETN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.087"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(808..1122)
                     /locus_tag="IXF98_002114"
     CDS             complement(808..1122)
                     /locus_tag="IXF98_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556415.1"
                     /translation="MKHPPKTTVEQLAESSGLSDKTIQRMRNGEAVVIQSIVAMCIGL
                     HLHPDISTEMLQKLGYTLGPAVEIHMIYKTLLCNCKTMTIEECNGILTNADFEPLTKA
                     EV"
     gene            complement(1289..4279)
                     /locus_tag="IXF98_002115"
     CDS             complement(1289..4279)
                     /locus_tag="IXF98_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1556416.1"
                     /translation="MKIQFDTLDYQTDAVNSAVRVFEGQTIKESNFTITDDYSRGSMF
                     TEDGIGVGNRVIINEEQMLKNVNKTQILDGIVPSDNLFGNNKAFPQFNIEMETGTGKT
                     FVYLKTILELNKQYGFLKFVIVVPSIAIKEGVLKSFEITKDYFKNQYNGVVYDLFMFD
                     SSKLDRVRSFASANTIDIMVTTIQSFNKDTNVMNRENDQLSGARPIDLIAETRPIVII
                     DEPQSVDNTDLAKDALKSLNPSAGFRYSATHRDNTYPKIYQLGAVDAYDNQLVKQIEV
                     AGIEMDEDGNRAHLKLIDVKTGKNGISAKIEVYKKTRNDADKITMNFKAGDDMFSKTK
                     LQAYDEVGFIQDIDATPGFESVTFSGNPEKITLESATLEDEAIKRAQISKTIEEHLNK
                     ELKFKQSGQRIKVLSLFFLDKVENYRVYDEEGNPSLGRYARIFEEEYERLIHLSKYKE
                     LNDVNVPVSEVHDGYFSADNKGKLKNTKGDTQADESTYEMIMKDKEGLLTFYDEEKHH
                     TAKANKIRFIFSHSALKEGWDNPNVFQIATLIETKDTINKRQKIGRGLRIAVNEDGER
                     VPGFAVNTLTVMANESYKDFAEGLQKEYEEDGVIFGVFNKDSFATIITHYDEVTEEME
                     ILGKEKSEQLFNYLKQEEFINSSGRATDKLKQAITDQEIEVPEEFIDVVPQILVAAKS
                     KVKSLDIKDAKDKVEVKVVKEALADDFLELWNKIKYKTTYKIHFDSNKLIEFAAKGNE
                     SFDGVDSIQTRKGTYLYKKGMVEMSQAGVKADESGESSVRATTSGMYKLPDIITFLQN
                     ETSLTRKTIVAILQSVNNLDSFKNNPLSYMNQAAKIINAIKNQLIVDGIEYTRTNDEY
                     EQTLFSTETLNAYLGEKGNSVKVDESKQKTVYDYVVTDSNIEKDFARKAEMDDNVKFY
                     IKLPDWFKIRTPLGPYNPDWALLYEEDDEQHLYFIVETKGDTSFEQLRPHERAKILAG
                     EKHFKAVDTDIKFKRVSDEAEIRG"
     gene            complement(4300..6144)
                     /locus_tag="IXF98_002116"
     CDS             complement(4300..6144)
                     /locus_tag="IXF98_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAQ1556417.1"
                     /translation="MINENNQVPSSSLDITEQNIEKLKELFPEVLTEKKIDFDKLRLI
                     LGDEVETAPERYSFTWNGKKQAMQLAQQPTVATLKPNKAKSKNWDETQNLYIEGDNLE
                     VLKILQKSYANKVKLIYLDPPYNTGSDFVYKDSFSDSLENYLEVTCQVDDEGKKFSTN
                     SDTDGRYHTNWLNMMYPRLKLARNLLRQDGVIAISIDDNEVAKLRNLLDETFGENNFI
                     QEIAWRRSDNQANIGLAARVKEYILIYAKNKKELVYGRLPLSESAIREYRYSDSYAGK
                     FRRGILLDKTRGRYSYPVTTKSGNVLNGPWMVAEEKFKELDENNGIYWTSGGEEQPYS
                     KIYLSESKGAIPNDFWGIEYGTNQRASLEVEVLFGERYFDFPKPVSLIKSLISLGAPD
                     KDDIVLDFFSGSGTTAQAVMESNIDDDGKRKFILVQLPEDIDAKHKAYKAGMRKIPEI
                     AEKRISLAGDKIIAENPELAGKLDVGFKVFELSKSNIKKWNAEPKDLNEQFELLANNF
                     EEGSKPIDVVYEIMLKQGLDLTYPISETKVGEAVVYDIAFGAMFVVLGDKITSEVASH
                     IIKQIADEEAENSVVVLQDEKFINDSEKLNTIEQLNASGIQYNDILSI"
     gene            complement(6172..6894)
                     /locus_tag="IXF98_002117"
     CDS             complement(6172..6894)
                     /locus_tag="IXF98_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HAQ1556418.1"
                     /translation="MSELETKDIIKWWKFPSATIINRNLPKTQIYPHMKNAADKQFLQ
                     DSVQSIYMLASLKTDNTHIAVYEDDKELYQEIQFLYVKTKDKGDSTKIYKMLAHLIPY
                     PLVILTDEPDSFTIYTGRFEKLSTGFLKLMNVYPSPVYQDENLEEVLQQISLIDLPRQ
                     NLKTFYDGLRDEIVTATAKSQYGEAVANITGEEKDQLDALKKQIEDLRGQIKKERQLN
                     RKIDMQMKLKKLKDELSSKINQ"
     gene            complement(6895..10152)
                     /locus_tag="IXF98_002118"
     CDS             complement(6895..10152)
                     /locus_tag="IXF98_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1556419.1"
                     /translation="MELKLIDNKGDNTLENLISEKVTKHSKISIQTAAFSIFAFHALQ
                     KEIKRSKGLRLVLTKSFFEKEETSFLKRYEIAQSEEDISGNKYEIKLRNKMNTAFIAR
                     ETARLIEEKVKVHQLGPNQNALDELLIENEADIEGNVVVPSMFKFTSDGLGLTESNNV
                     SSMTGIIGEPNHLAGLKADFDAVWNDPKKSKEVTDKVLEKVRTIYQENPPEWIYFVSL
                     YHIFHDKLSELDEQDIAPEGSGFKDSVIWNKLYQFQKDGVMGLIRKLEKYNGAILADS
                     VGLGKTFSALAVIKYFELQRKRVLVLAPKRLRDNWTLYTRPDVRNVLVDDYFNYTVLN
                     HTDLSRYKGESDGIKLDTFLWSDFDLVVIDESHNFRNNDTSVNRETKTRYQRLMEDII
                     QKGRKTKVLMLSATPINNRMNDLKNQIGFITEGKTDALAKYDVDDIDTTLRLAQQAFN
                     QWMELDESERTTQSFLDKVNPDYFKLLDMLTIARSRKHIEKYYDTTSLGDFPERLKPM
                     TVKPNIDSSGQFPNLHDVYDSIGYLNMSMYQPLQYVLSTKRKRYEELYDTKVKEGKSS
                     FRQVDREQSLAKLIRANLFKRLESSIYSFGSTLDRMVGGIEDILEKIKEHQAGNVEMP
                     SITDFDDEDLESAFDEQAVGTKTKILIGDMDLVKWSQALNEDLEKLKELQTVTHRVTP
                     DKDDKLMDLEKILKHKYEHPINGNNKKVIIFTAFADTANYLYENLADKVKRLYGLNSA
                     LVTGGSTPNKTTMKGVAVNDMSDILTNFSPISKGRDQINPNATEEIDLLIATDTISEG
                     QNLQDADYLINYDIHWNPVRVIQRFGRIDRIGSRNKQIQLVNFWPNVDLDTYLNLEQR
                     VKGRMVMLNTAATGEDDLLNIKSNKEMNDLKYRKKQLEQLQTQVIDLEDVNGAISITD
                     MTFNDFKADLQNALKEHEKLLNEAPLGMYAITDTTNFPEAEPGVILTLKQQTNDLGQE
                     NSILPYIVVYMRMDETVKINYMYAKQVLDFFKKLTVGKKTVNQPLVDEFYEETNGGKD
                     MSAYSEILSKAIEAIRGKQDEVGMGSLFSPGGTSVQTELLDDLDDVELISFLIIR"
     gene            complement(10201..10401)
                     /locus_tag="IXF98_002119"
     CDS             complement(10201..10401)
                     /locus_tag="IXF98_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556420.1"
                     /translation="MMSYKKLWKLLIDRDMSKKDLAELADLSQYTINKLNNSGTVTTE
                     TLTKICIALNCDVGDICEVVAA"
BASE COUNT         3252 a         2363 c         1776 g         3877 t
ORIGIN      
        1 aagtaaatct atttttttaa acgtagattc acaatttcat taaatttttg taatgtttcc
       61 tcatcaggta aaataacttc tagttcgatt atcatccctt tcgttaaata cttaataatt
      121 gaccctgaag atctagaagt aattttattt aaatattagt aatcaaacat tttatttgag
      181 ttcgattcta ttatataatt tcgatgataa gcttcaaatt taccctcgaa tctattaaac
      241 caacactctc catttccaac cagtatcatt gcctttgtat caaaagagta tgatggatat
      301 tttagatgtt ctgagctaaa aatgtaacat ggatattgac catcttaggt agcgtgatta
      361 gcatcttttt tgccggttgt tattgttgtt aaatccccta atttttttcg attccattct
      421 ttcggtagga cttgtttatt tttagaagct agttgtccat cttcactagt taaattggaa
      481 tctacaaacc atttatgaaa tgtcgaaagg tatagaaaat aagcattttt actggggtta
      541 accagcagta atgttctatt tctattcctt gcccttattg caaatcgggc tctgaatatt
      601 aatttttatc gtgactaatt ccttagtact cccttatcat atctagattg agcgctgtaa
      661 accatgacac ttactgtccg ttaatttcgt ctaaaacacg caaaaaaaga cctgattcaa
      721 agatataaaa tctctaaatc aggtcttcat tgagtttaca aaccggacag taagtgtctg
      781 attctcataa aaataaattt aaaatcttta aacttcagct ttggttaacg gttcgaagtc
      841 agcattagtc aaaattccgt tgcattcttc aattgtcatc gttttacaat tgcaaagcag
      901 cgttttataa atcatatgaa tttctaccgc tggccccaac gtatatccga gcttttgaag
      961 catttctgta ctaatatcag gatgaaggtg caaaccaata cacattgcga caattgattg
     1021 aataactaca gcttctccat ttcgcattcg ctgtattgtt ttatcagaaa ggccagatga
     1081 ttctgccagc tgttcaacgg ttgtctttgg tggatgcttc atcaacacct taagcgcatc
     1141 accgaagcta agtggcgcaa tttcattcta tacttgtttt aaaccttttt gatctttctt
     1201 caattaaact gatgcatatt aacatagttt ttaaattaac gttgctgtac cattgattta
     1261 cttctccctg ttttatttca aatatagatt acccacgaat ctccgcttca tcagaaaccc
     1321 gcttaaattt aatatctgta tcaactgctt taaagtgttt ctcaccagcc agaatcttag
     1381 cccgttcatg aggacgtaac tgttcaaaac ttgtatcacc tttcgtttca acgatgaagt
     1441 ataggtgttg ctcatcatct tcttcatata gcaatgccca gtctggattg taaggcccaa
     1501 gcggtgtgcg aatcttgaac caatctggca gcttaatgta gaacttaaca ttgtcatcca
     1561 tttcagcctt acgagcaaaa tccttttcaa tgtttgaatc agttacaaca tagtcataaa
     1621 cagtcttttg tttactttca tcaactttaa ccgaattccc tttttcaccg aggtaagcat
     1681 tcaaggtttc agtgctaaac agcgtctgtt catattcatc atttgtacga gtatattcaa
     1741 taccatccac aatcaattga ttcttgattg cgttgataat cttcgcagcc tgattcatat
     1801 atgacaatgg attgttctta aaactatcca gattgttgac tgactgcaaa attgcgacga
     1861 ttgtcttgcg agtcaaactt gtttcatttt gcaagaaagt aatgatatct ggcaatttgt
     1921 acataccact cgttgttgca cgaacactgc tttcgccaga ttcatcagcc tttacaccgg
     1981 cttgactcat ctcaaccatc cccttcttgt aaaggtaggt ccccttgcga gtctgaatgg
     2041 aatcaactcc gtcaaatgat tcatttcctt ttgcagcaaa ttcgattaat ttgtttgaat
     2101 caaaatgaat cttataagtc gtcttgtatt tgattttgtt ccaaagttct aaaaagtcat
     2161 ctgctaaggc ttcttttaca accttgactt cgactttatc cttggcatct ttgatatcaa
     2221 gactcttaac cttagattta gcagcaacca aaatctgtgg cacaacatca ataaattctt
     2281 ccggtacttc aatttcctga tcagtaatcg cttgtttcaa cttatcggtt gctcgcccag
     2341 atgaattaat aaattcttcc tgtttgaggt agttgaacaa ttgctcagat ttttctttgc
     2401 caagaatttc catttcttcc gtaacttcat cgtaatgcgt aataatcgtc gcaaagctat
     2461 ctttattaaa tacaccaaag ataacaccat cttcttcgta ttctttttga agaccttcag
     2521 caaaatcctt atagctttca tttgccataa cagtcagcgt gttaacagca aatccaggga
     2581 cacgttcacc atcttcgtta accgcaatac gcaggccacg accaattttc tgacgtttgt
     2641 taattgtatc ctttgtttca atcaaagtgg caatctggaa tacattcggg ttgtcccaac
     2701 cttccttcaa agctgaatga gagaaaatga agcgaatttt attagccttc gctgtatgat
     2761 gcttctcttc atcatagaaa gtcagtaagc cttccttgtc cttcataatc atctcgtagg
     2821 tagattcatc ggcctgagta tcacccttgg tattttttaa cttaccttta ttatctgctg
     2881 agaaataacc atcatggact tcggatactg gcacattcac atcgttcaat tctttgtact
     2941 tggacaaatg aatcaagcgt tcatattctt cttcaaaaat acgagcataa cggccaagac
     3001 taggattacc ctcttcatca taaacgcggt agttttcaac cttatccaag aagaacaaac
     3061 tcaacacctt gattcgttgc cccgattgtt tgaacttcaa ctccttgttc aagtgttctt
     3121 caatcgtctt agaaatctga gcacgtttta ttgcttcatc ttctaatgta gctgactcaa
     3181 gcgtgatttt ttcaggatta cccgagaaag taactgattc aaatccaggt gtagcatcaa
     3241 tgtcttgaat gaacccaact tcatcatacg cctgaagctt ggttttacta aacatatcgt
     3301 caccggcttt aaagttcata gttattttat cagcgtcatt acgtgtcttt ttataaactt
     3361 caatcttggc actgataccg ttcttaccag tttttacgtc aatcaatttc aaatgagcac
     3421 ggttaccatc ttcgtccatc tcgatgccgg ctacctcaat ctgcttaacg agttgattgt
     3481 cgtaagcatc aaccgcacca agttgataaa tcttcggata agtattatct cggtgagtgg
     3541 ctgaataacg gaagcccgct gacggattta aactcttcaa agcatcttta gccaagtcag
     3601 tattatcaac agactgtggt tcatcaataa taactatcgg ccgtgtttca gcaatcaggt
     3661 caattggtcg tgcaccagaa agttggtcat tttcacggtt catgacgttt gtatctttgt
     3721 tgaaagattg aatggtcgta accatgatat caatcgtatt tgccgatgca aatgaacgaa
     3781 cacggtcaag tttactgcta tcaaacatga ataaatcata aaccacaccg ttgtattgat
     3841 ttttgaaata gtcttttgta atctcgaagg atttaagtac cccttcctta atcgcaatac
     3901 ttggcactac aatcacgaat ttcaaaaatc cgtattgttt gtttaattca agaatcgttt
     3961 tcaagtaaac aaaagtctta ccagtccctg tttccatctc aatattgaac tgcgggaaag
     4021 ctttgttatt tccaaacaga ttgtcacttg gcacgattcc atccagaatt tgagttttgt
     4081 tgacgttctt caacatctgt tcttcattaa taatcacacg gttaccaaca ccgattccat
     4141 cctcagtgaa catcgaacct cgtgaataat catctgtaat cgtaaaattt gattctttaa
     4201 ttgtctgacc ttcaaacacg cgcaccgcac tgttaacggc atctgtttga tagtcgagtg
     4261 tatcaaattg tattttcaag agtctttacc tcctttgctt taaatactta aaatgtcatt
     4321 gtactggatg ccacttgcat tgagctgctc aatcgtattt aacttctcac tatcattgat
     4381 gaatttctca tcctgtaaaa caactactga gttttcagct tcttcatcag caatttgctt
     4441 aataatatga cttgccactt cgctcgtaat cttgtcacca agtactacaa acattgcacc
     4501 aaaggcaata tcataaacaa ccgcttcccc aacttttgtt tcactaattg gataagtcaa
     4561 atcaagacct tgcttcaaca tgatttcata aacaacatcg attggttttg aaccttcttc
     4621 aaagttgtta gccagtaatt caaactgctc attcaagtct ttgggttctg cgttccattt
     4681 cttgatattt gatttagaga gttcaaaaac tttgaaacca acgtcaagtt ttcctgctag
     4741 ttcaggattt tcagcaatta tcttgtcccc ggctaaagag atccgttttt ctgcaatttc
     4801 tggtattttc cgcattcctg ccttgtatgc tttatgtttt gcgtcaatat cttccggcaa
     4861 ttgaacgaga ataaactttc tttttccatc atcatcaata ttggattcca ttaccgcttg
     4921 tgctgtagtt ccagagccag aaaagaagtc taggacgata tcatctttat ctggtgcacc
     4981 aagtgaaatc aaacttttga ttaatgaaac aggctttgga aagtcaaagt atctttctcc
     5041 aaataaaacc tctacttcta aactagctcg ttgattcgtt ccatattcaa ttccccaaaa
     5101 gtcattcggt attgcacctt tactttcaga caagtaaatt ttgctatatg gttgttcttc
     5161 accaccgctt gtccagtaaa tgccattatt ttcatctagt tctttaaatt tttcttccgc
     5221 aaccatccaa ggaccattta aaacatttcc agattttgtt gttactggat aagagtagcg
     5281 tccgcgagtt ttatccagta aaattcctcg tcggaacttt cctgcataag aatcagagta
     5341 gcgatactcc cgaatcgcac tttctgataa aggcaatcgt ccataaacta attccttctt
     5401 attttttgca tagattaaaa tatactcttt cactcgtgca gctaaaccaa tattagcctg
     5461 attatcagag cgtcgccatg caatctcttg aataaagtta ttttcaccaa aagtttcgtc
     5521 aagtaaattt ctaagctttg caacttcatt atcatcaata gaaattgcaa taactccatc
     5581 ttgtcttaaa agatttctag ctaacttaag acgtggatac atcatattca accagttagt
     5641 atgatatctt ccatctgtat ctgaattagt tgaaaacttt ttcccttcgt catcaacctg
     5701 acaagtaact tctaaataat tctcaagcga atcagaaaaa ctatccttat acacaaaatc
     5761 actacccgtg ttgtagggtg gatccaaata tatgagtttc actttattgg cataagactt
     5821 ttgcaaaatc ttcaacacct caagattatc tccttcgata taaagatttt gtgtttcatc
     5881 ccagttcttt gactttgcct tatttggctt caatgttgcc accgttggct gttgagccag
     5941 ctgcatcgct tgtttcttgc cgttccaagt aaaactgtag cgttcgggtg ctgtttcaac
     6001 ttcatcccca agaatcaaac gtaacttatc aaagtcgatt ttcttttcag ttaatacctc
     6061 aggaaataat tccttcagct tttcaatatt ttgttcagtg atatcaagag aacttgaagg
     6121 cacttggtta ttctcattta tcataaatag gatttccttt cacactttca cttactgatt
     6181 aatcttgctt gaaagctcat cttttaactt tttcagcttc atctgcatat caattttgcg
     6241 attcagttga cgttcctttt taatttggcc acgcaaatct tcaatctgtt tctttaatgc
     6301 atcaagctgg tctttttctt caccagtgat attagcaact gcttcaccat attgtgactt
     6361 agccgtagct gtcacaatct catcccgaag tccatcataa aaagttttca gattctgccg
     6421 tggtaaatca atcagtgata tttgctgcaa cacttcttcc aagttttcat cttgataaac
     6481 cggggatgga tacacattca tcaacttcaa aaagcctgtt gataacttct caaagcgacc
     6541 cgtgtagatc gtaaaactat ccggttcatc ggtgaggata actaatggat atggaatcag
     6601 gtgtgccaac attttataga ttttagtcga gtcaccttta tccttggttt tcacataaag
     6661 aaactgaatt tcttgataaa gttccttgtc gtcctcatac acagcaatat gtgtattgtc
     6721 agttttcaga cttgccaaca tataaatgct ttggacggaa tcttgtaaga attgcttgtc
     6781 cgccgcattt ttcatatgtg gatagatttg cgtttttggt agatttcgat tgatgattgt
     6841 agcacttgga aatttccacc atttgattat gtctttcgtt tccaattcgc tcacctacct
     6901 aataatcaaa aatgaaatta actcaacatc atctaaatca tccaatagtt cagtttggac
     6961 gctagtacca ccaggtgaaa acaatgaacc catccctact tcatcttgct tacctcgaat
     7021 tgcttcaatc gctttactca agatttcaga ataggcactc atgtccttac caccattcgt
     7081 ttcctcataa aactcatcta caagtggttg gttgaccgtc tttttaccga ccgtcagctt
     7141 tttaaagaag tccaacactt gcttggcgta catgtagttt attttgactg tttcatccat
     7201 tcgcatataa acaacaatgt acggcaagat actgttttct tgtcccaagt cattcgtctg
     7261 ctgcttcaag gtcaatatga cacccggttc agcttcaggg aagttggttg tatccgtaat
     7321 tgcatacatg cctagcggcg cttcattcaa caacttctca tgctctttca aagcattctg
     7381 caagtctgct ttgaagtcat tgaaggtcat gtcggtaatg gaaattgccc cattgacgtc
     7441 ttccaaatca atgacttgag tttggagttg ttccaactgt ttcttacggt atttcaagtc
     7501 gttcatttct ttattagatt taatattcaa taagtcatct tcaccggttg ctgccgtatt
     7561 taacatgacc atacgtccct tgacacgttg ttccagattc agataagtat ccaagtccac
     7621 attcggccag aagtttacta gttgaatctg tttattacgt gagccaatac ggtcaatacg
     7681 cccgaaacgt tgaatgacac gcactggatt ccagtgaata tcgtagttaa tcaagtaatc
     7741 tgcatcctgc aagttttgcc cttcagagat ggtatccgtt gcaatcaaca aatcaatttc
     7801 ttcggttgcg ttcggattga tctggtcacg cccttttgat atcggtgaga agttcgtcaa
     7861 gatatcactc atatcattga cggccacacc tttcatggtt gttttgttcg gtgtagaacc
     7921 acccgtcacc aatgcagaat tcagaccata aagtcttttt actttgtcag ctaagttctc
     7981 atagagatag ttagccgtat ctgcaaaggc agtaaagatg attactttct tgttgttgcc
     8041 attaattgga tgctcatact tatgcttgag aattttctcc aagtccatca acttatcatc
     8101 tttgtccggt gttactcgat gcgtgacggt ttgcaattct ttcaactttt caagatcttc
     8161 attaagagct tgggaccact tcaccaagtc catatcacca atcaatattt tagtttttgt
     8221 ccccactgcc tgttcgtcaa aggcactttc caaatcctca tcatcaaaat cagtgatact
     8281 tggcatttcc acgttacccg cttgatgctc tttgattttt tcaaggatat cttcaatccc
     8341 gccaaccatt cggtcaaggg ttgatccaaa cgaataaatg gaactttcca atcgtttgaa
     8401 caaattggca cgaatcaatt tagccaagga ttgttcacgg tcaacttgcc tgaatgacga
     8461 cttcccttct tttaccttgg tgtcatacaa ttcttcgtag cgctttcttt tagtcgagag
     8521 cacatactga agtggttggt acattgacat gttcaagtaa ccgattgaat cataaacatc
     8581 atgtaagtta gggaactgcc ctgatgaatc aatatttggt ttcactgtca ttggcttcag
     8641 gcgttcaggg aaatctccca atgaagtcgt atcataatat ttttcaatgt gtttccgact
     8701 acgagcaatc gtcaacatat ccagtaactt gaaataatcc ggattgacct tatcaagaaa
     8761 gctttgagtc gtccgttcag attcatccag ttccatccat tgattaaaag cttgttgtgc
     8821 taaacgcagt gtcgtgtcaa tatcatcaac atcatacttc gccaacgcgt ccgttttgcc
     8881 ttcagtgatg aaaccaatct gatttttcaa gtcattcatt cggttgttga ttggtgtagc
     8941 tgaaagcatc aatacttttg tcttacgtcc cttttgaatg atatcttcca tcaagcgttg
     9001 ataacgtgtt ttggtttcac gattgacgct ggtatcatta ttacggaagt tgtgggattc
     9061 atcaatgaca accaaatcaa aatcagacca cagaaaagta tccagcttga ttccgtcact
     9121 ttcaccttta taacggctaa ggtcagtgtg gttcaaaaca gtgtagttaa agtagtcatc
     9181 cactaaaaca tttcttacat caggtcgtgt ataaagtgtc cagttatccc gtagtcgttt
     9241 tggagcaaga acgagtactc gtttacgttg caactcaaaa tatttgataa cggccaaggc
     9301 tgagaaagtt ttacctaaac caactgaatc tgctagaatc gcaccgttgt atttttcaag
     9361 cttacgaatt aagcccatca caccatcttt ttgaaattgg taaagcttat tccaaattac
     9421 tgaatccttg aacccagaac cttctggcgc aatatcttgc tcatccaatt cactcaactt
     9481 gtcatggaaa atatggtaaa gcgatacaaa ataaatccat tcaggtggat tttcttgata
     9541 aatggttctg actttttcaa gcaccttgtc tgttacttct tttgactttt tagggtcatt
     9601 ccagacagcg tcaaagtcag cttttaaacc agctaagtga ttaggttcac caataattcc
     9661 cgtcattgaa gaaacgttat ttgactctgt aagtcctaag ccatctgaag taaatttgaa
     9721 cattgaagga actaccacat taccttcaat atctgcttca ttttcaatca gtagttcatc
     9781 caaagcattt tgatttggtc ccagctgatg aacctttact ttttcctcaa tcaaccttgc
     9841 cgtttcacga gcaataaaag ccgtattcat tttatttctg agcttaattt catacttatt
     9901 tccagaaata tcttcttcac tttgcgcaat ttcataccgc tttaaaaaac tagtttcttc
     9961 cttctcaaag aaagatttcg tcagaaccaa acgcaacccc tttgaacgct tgatttcctt
    10021 ttgtaatgcg tgaaaagcaa aaattgaaaa tgcagcagtt tgaatactga ttttcgaatg
    10081 ttttgtcacc ttttcactga ttaaattttc tagggtgtta tcccccttgt tatcaattaa
    10141 ttttaattcc atgagcgtct cccacaaggc tataatttta attctttatt ctgtgctgta
    10201 ttaagcagct accacttcac aaatatcccc aacatcacaa ttcagtgcga tgcaaatttt
    10261 agttagtgtc tcagttgtca ctgtgccact attatttaat ttgttgatgg tgtattgact
    10321 aagatcagcc agttcagcta aatctttctt tgacatgtct cggtcaatta ataacttcca
    10381 caatttttta tagctcatca tcttatcacc tattttctct acaatctgat atgccgataa
    10441 taaatatatt atatcacgat tgatatgaaa ttttacataa aaatatagcg aacacttaat
    10501 tattttacga ttgattagat tttgataaat atttatagga acgtaaattt tagggggtgt
    10561 ttaggggtgt catagggtgt taattcaaaa cctaggggtg ttatattttt atccccttgg
    10621 aaattatgcc tgtatctcat tgatgaaatt catcgtaaaa taaaaaagtt tctcaagcca
    10681 caagctcaag aactcccgta ttatcgcctt ttctagcagt ttgtatttgt atcgattgcg
    10741 tggttttaat atcaatgtaa attagcttta cctttggaaa gcattaatag aaatgactga
    10801 agctagtaag gaactggcag ctaagttaac tgaaaatagt tatttagctt atattaatga
    10861 aaaatccaca cgagaattat ggacactcga tgactaacaa tatccttatt agtatctatc
    10921 cacgatacat ggaaaaaatt ataaacaaac aaaaaaactt tgaatttaaa atttctatct
    10981 tacctcagct ccttataata tcttttggat ttacgaaaca atcccaccaa agctttaaag
    11041 tataaattga ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta
    11101 ggagacgatg agtttgaaca attagtttaa aagggaaaat atgcttacga aattatccac
    11161 ctagaagaaa tacttttacc aataagtctg tctaatttaa aaaatttagg agttactgca
    11221 ccacaaggat atgcatatat aaaaaaatat cctagtcttg tcgatatt
//
LOCUS       DACETN010000085        11253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DACETN010000085 DACETN010000000
VERSION     DACETN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.088"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(40..1419)
                     /locus_tag="IXF98_002120"
     CDS             complement(40..1419)
                     /locus_tag="IXF98_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1556421.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IXF98_002121"
     CDS             complement(1440..2987)
                     /locus_tag="IXF98_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1556422.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IXF98_002122"
     CDS             complement(3236..4015)
                     /locus_tag="IXF98_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1556423.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IXF98_002123"
     CDS             complement(4090..4548)
                     /locus_tag="IXF98_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1556424.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IXF98_002124"
     CDS             complement(4560..5819)
                     /locus_tag="IXF98_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ1556425.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IXF98_002125"
     CDS             complement(5935..6249)
                     /locus_tag="IXF98_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1556426.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IXF98_002126"
     CDS             complement(6395..7891)
                     /locus_tag="IXF98_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1556427.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IXF98_002127"
     CDS             complement(8069..8548)
                     /locus_tag="IXF98_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ1556428.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IXF98_002128"
     CDS             complement(8924..9169)
                     /locus_tag="IXF98_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556429.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IXF98_002129"
     CDS             9274..10047
                     /locus_tag="IXF98_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556430.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IXF98_002130"
     CDS             complement(10276..10524)
                     /locus_tag="IXF98_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1556431.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IXF98_002131"
     CDS             complement(10623..10937)
                     /locus_tag="IXF98_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556432.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DACETN010000086        11091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DACETN010000086 DACETN010000000
VERSION     DACETN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.089"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            30..653
                     /locus_tag="IXF98_002132"
     CDS             30..653
                     /locus_tag="IXF98_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556433.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1103..2152
                     /locus_tag="IXF98_002133"
     CDS             1103..2152
                     /locus_tag="IXF98_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1556434.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2149..3030
                     /locus_tag="IXF98_002134"
     CDS             2149..3030
                     /locus_tag="IXF98_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556435.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3033..3803
                     /locus_tag="IXF98_002135"
     CDS             3033..3803
                     /locus_tag="IXF98_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1556436.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4082..4936
                     /gene="aroE"
                     /locus_tag="IXF98_002136"
     CDS             4082..4936
                     /gene="aroE"
                     /locus_tag="IXF98_002136"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1556437.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5114..6277
                     /locus_tag="IXF98_002137"
     CDS             5114..6277
                     /locus_tag="IXF98_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1556438.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6394..6804
                     /locus_tag="IXF98_002138"
     CDS             6394..6804
                     /locus_tag="IXF98_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1556439.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6978..9206
                     /gene="topB"
                     /locus_tag="IXF98_002139"
     CDS             6978..9206
                     /gene="topB"
                     /locus_tag="IXF98_002139"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1556440.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9344..9661
                     /locus_tag="IXF98_002140"
     CDS             9344..9661
                     /locus_tag="IXF98_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556441.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9821..10057
                     /locus_tag="IXF98_002141"
     CDS             9821..10057
                     /locus_tag="IXF98_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1556442.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10054..10452
                     /locus_tag="IXF98_002142"
     CDS             10054..10452
                     /locus_tag="IXF98_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1556443.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3874 a         1834 c         2304 g         3079 t
ORIGIN      
        1 aaaaaatagg agaaaaagta gaaaagaagg tgtttgttat ttgggaagta gttaaatcga
       61 gagtacaaga aatgaatgaa catttctcag tgtcattcgc agaaaatcca agtaagtatt
      121 ttcgacgtct tgtttttctg ctcatcttat cattgctgtt aacagttgca atgtttttat
      181 tgccggaaaa aatccgagaa gagcaagtaa atagtctcgt ttattcgtcc ataccaacca
      241 gcaaattgaa accttttgat tacagcaaag ctgatcagaa aatcaaacaa agccgggcga
      301 tatcggtgat gttcagcaaa ccgaacggga aaacctatca ggacatgctt gatgttttta
      361 atgatcctga gcttatggaa gaattgaatc gaccgatctt ttattatccg atcgtttatg
      421 atgtacatga actggaacaa aaatataaca tacgaacgga tgaagtgaca tttattttct
      481 ttgatggagg aaaagaagca aatcggataa ctgcgggtaa gggaggaata actgattttg
      541 ataaggagtt gatccctgaa ctgaatcgac ttccgttagc caatatcaaa caattagaag
      601 aagaattgga tagaacggga gtaacttccc aaaattccga gattgctaat tgagacattg
      661 gcgcgatgaa acggtgggtc tatatagcgc tatttttaat tatcagaaca agtgatgttt
      721 gatcttcaaa ataacaaata aaaaattaaa aaatatagtt gacgaaattc aaaaattcga
      781 ttagacttaa atcaattctt aaggaaaggg tgacctgtaa tgaacttatc aaaacgtatt
      841 tcagcagaaa acaaaaaaaa ttggcgcagc tggcgtagat aagttgtatt aatggtgaga
      901 ctttcccata gatacaactt tttagagtgc atttctaaag cttgtatcta tgctagacat
      961 gaacattgac tgccgcatag attagaatct atgcggtttt ttgtgtaaag acaatgaccg
     1021 ctttggccat tgtctttttt tgtagactgg atacttgcct gattataata aataaaaaga
     1081 ttgactttgc aggaggaaaa aaatgaaaaa caaacgatta ataacagttg tagtaattat
     1141 tttagtttat ttagcaggtg catttattta tggaaaagcc tcaggcgaaa aagaaagtac
     1201 ttcgacgaac cagacaaaaa aaacaacagt tgttggggta ttgcaatacg tgagccatcc
     1261 ggcattagat gagatttatc gaggaatcaa agatggtctg aaagaagaag gattaaaaga
     1321 aggagaaaat cttgagatca aatttcaaaa tggacaagcc gatcagagca agctagccac
     1381 aatgagtcaa cagctagtac aatctgatcc agatgccttg attggaatcg caacacctgc
     1441 cgctcaatca ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg ctgtaacgga
     1501 tcctgtgggg gctggattag tcgcttcttt agagaaacct ggtggtaatg taacaggtgt
     1561 ttctgatcag ccgcctgtta cttcacaaat caagcttgct tctgatttgt tgccaaatgc
     1621 aaaaaaagtc ggaatcttgt atgcttcttc agaggataat tcaaaatacc aagtagcaca
     1681 agcagaagaa gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg tgccttccac
     1741 taatgagatt gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta tctatgttcc
     1801 attagataat accattgcca atgcgatgca aacagtggta aaagaagcaa acaaagcaaa
     1861 tatccccgtc attccatctg tggatacaat ggtggaacaa ggtggactag caacaatcgg
     1921 gatcaatcaa tatcaattag ggcttcaaag cggtaagatg gcggcagaac ttgcgagtgg
     1981 aaaagaaaaa ccggaaacga cgcctgttta tacatttgat caaggggata cagtgatcaa
     2041 tcaatctcaa gctgatcgct taggaatcat tgttccccaa tcgatgaagg aaaaagccaa
     2101 aatcattaca gatgactcgc agcaagaaac atctaaggag gatgacaaat gatcgtatca
     2161 gctatttcac aaggcatgct ttgggcaata ttaggattag ggattttcat gacttatcgg
     2221 atcttagact ttcctgatat gacgacagaa gggtccttcc cattaggtgc tgctgtctgt
     2281 gtgacagcta ttactcatgg ggtagcgccg attgtagcta cattattagg cgtaggtgct
     2341 ggcatgatag caggactcgt tacggggtta ctttatacaa aaggaaagat accggttatt
     2401 ttagcaggga ttttagtgat gtcaggattg aactcagtta ttttgtttgt gatgcgaaca
     2461 ccaaatcttt ctttattgaa tcatccgatt ctacaagaca gcttccatcg ctttgggtta
     2521 cctgattact ttgacacagt ccttcttggc ttcatctctt tgggcatctt gattgctgta
     2581 ctgctattct tcttcaatac agatttgggt caaggctata ttgcgacagg agacaacgaa
     2641 acgatggctc ggtcgttagg gataaaaacc gatcgtatga aaattttagg tttaactttg
     2701 tcgaacggtg tcattgcatt atccggtgcg ctgattgccc agaacgacgg atatgcagat
     2761 gtaaataaag ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat cggtgaagtg
     2821 atcttccaag aattgacgct agcagaaaga ttgattgcta ttgttgtcgg cagtatcatt
     2881 tatcagctat tgattttagt agtaatcaaa ctagggtttg acacgactta tctgaagttg
     2941 ttctccgcta tcatcttagc tatttgtctg atgatcccgc aattgaagaa agcactgaac
     3001 ttaaaaacgg gttttgagaa ggaggcatag taatgactga tatattgaca atcaaaaatg
     3061 gcagaaaagt cgtaaacaat ggaatgaatg aagagaaagt actattggat gaaattaacc
     3121 ttacaatcag agaaggggac ttcatcactg tccttggcgg gaatggtgct ggaaaaagca
     3181 cactgttcaa tacgatcgct ggtacgcttc aactcagtca aggatcgatc tcttttaaag
     3241 atcagctgat cacaaaagat tcagaagaaa aaagagcacg attcctttct cgagttttcc
     3301 aagaccccaa gatgggaacg gctcctcgga tgacagtagc agaaaatttg ctacttgcac
     3361 aaaaaagagg tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa aaagaagatt
     3421 ttttcagact atgtcaagaa gtcggtaatg gcctagagca acacttggat actccagcgg
     3481 gtgaattatc tggtggtcaa cgccaagcat taagcttgct gatggcgacg atcacagctc
     3541 ctgagttact attattagat gaacatactg cagctttaga tcctaaaaca gctaaagcat
     3601 taatgcgatt aacaaatcag cggatcaatg aacaaaaatt gacttgtctc atgatcacgc
     3661 atcgaatgga agacgccttg aaatacggaa atcgtctgat cgtactccaa aaagggaaaa
     3721 tcgttcaaga tatttctgaa aaagaaaagc aacatttgac aatgggcgac cttttgcagt
     3781 tctttgaaga agtagtggat tgatttcgac agaaaaattc ctttcagtag aaggacttga
     3841 aaactcattg acatcttcct ttgattcatc ctatacttaa aacaataaac aattaaacat
     3901 atttgggtgt tttgtaggat cacgtagtca atgattttaa caattaaata gaatattctt
     3961 tgctctgaca agtaaacgat gagaaaagaa tttgctactt tgccacctag acgtttcgta
     4021 ctttttcgta tcaaacgcta agtggctttt tttattgaag taagaacaaa ggaggaataa
     4081 gatgatttct ggacaaacca aattagctgg gctctttgct gatccggctt ctcacagttt
     4141 atccccattg atgcacaata ctgcttttga agcaaacaac atcgatgcgg tgtatttagc
     4201 tttccaagta aatccaatga atttgaagca agcagtcgaa agtattcgaa cttttgatat
     4261 gttaggtgta aatctttcga tgccgaataa aacagcggtt attccttatc tggatgaggt
     4321 aagccaggaa gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa agggtcgttt
     4381 gattggctat aataccgatg gtgcgggctt catgcaatcc gtgcaagaag ctgggatttc
     4441 gatcaaaaag aaaaaaatga ctctactggg agctggaggt gcagcaaagg cagtcactgt
     4501 tcaagctgcg ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca accaaacatt
     4561 cgctcaagta aaagaaacat tcgaacaact cagctatcga acttcttgta agataaaagt
     4621 tcgtgacttt gccgatacgc atcaattaag aaaagacttg aaagaaagct gtcttctagt
     4681 caatgcaaca gatatcggga tgggaaagaa aatcaatcag tcaccgctct tagatataag
     4741 catgatgcat gagaatctag cggtttttga tttgatttat gcacctagag aaacacgttt
     4801 attaaaagaa gcaaaaaaag taggagcaaa aggatacaat ggacttggca tgttgatcta
     4861 ccaaggagca atcgcgtttg aattatggac aaaggaaaag atgccgatcc atgtgattga
     4921 aaagctgttt gattaaaaaa ggataataga taaagatctt ttattctcgc ataaacggtc
     4981 tcaagaaaag taatccttta tgtatcgaag catttatttc gttatttttt attaaattat
     5041 caaaaaatga taggcttttg tcgattggtt atcgtatcct ttatttatca gtaagaaacg
     5101 aggaggaacc acaatgagtc ggatggatcg ctataaggac atccataaga aggcgaaacc
     5161 tctgaagcgg gatacgggga gtatttttgc tagaagagaa cgaaaacgaa cgcgtatgga
     5221 aaatgaggaa gatgctaccc gtatttatga aagagagcag cattcatttg aacatttgaa
     5281 ccaatacgag caacagatat acagagatgc accaagagca gaagaacaac aaaaaaataa
     5341 aaaatttttc aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa agcgatcgtg
     5401 gaaaaaaatc attctatcga ttttgcttgt tttgatcctt tattcaggta tctcattttt
     5461 tattggtaaa tatgtagctg aacatgacac aagcttagag gcagcgactt cagaaacctt
     5521 tcatgggcaa aaagcgacga atggtgcgca taatattttg atccttggaa gtgatacacg
     5581 cggagaagat gccgggcgag ccgatacgat catggtctta cagttggatg gtccttccca
     5641 taagccgaaa ctgatttctt ttatgcgtga cagcttcgtt acgatcccag gggtaggaca
     5701 aaataagatc aattcagctt atgcatacgg aggagccgat ctagtgcgtc aaactttagt
     5761 agaaaatttt ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat cgtttgaaaa
     5821 agtgatcgat gccttattca tgaacggagt gaagatcgat gcagaaaaag atctgaatct
     5881 ggatggtgta gatattaaaa aaggggtcca gaagatggat ggacatgttc ttttgcagta
     5941 cgcacgtttc cgaatggatg aacaaggaga ctttggccgt gtcagacgcc aacagcaagt
     6001 aatgaacgct attttcagcc aattgaagaa tccattgaat ctgatccgtg caccttatgc
     6061 agctggtaaa gctttaggat atacatccac agatgtcact gcttggttct tgacgaaaaa
     6121 cctcttttcg attgcacgag gagctggtgg cattgatcga ataagtgtgc cagtagacgg
     6181 ttcgtggaat tatgggcaaa gtgattatgc tggcagtatt cttgaaattg ataatcaaat
     6241 gaatcaatct gctattacag attttttgaa taaatgatca taaaaaagat caagaagaag
     6301 ctgcgcaagg cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa cgctttctta
     6361 tggtagattt aaccaagaga ggagctgttt acattggata tctatctgaa cttcaaaaat
     6421 gagtcgcgtg aagccatcgc tttttatgaa aaagtttttc agactgaagc atcaggtatc
     6481 accacatacg gtgaaatgcc tggaatagaa caaatgaact tgccagaaga agcaaaaaat
     6541 ctaatcacga atgcacggct gaacatttat gatacattag ttatgtttgc agatacgcca
     6601 gaaggaattg gtcctgaatt agttgtggga aataatgttt ctttagtcat ccagacaaga
     6661 aatgaagaag aaacgaagcg cctatttgaa agcttagctg agggtggtaa gattgagatg
     6721 ccactggatg ctgttccgtg ggctcagttg tatgggatgg taactgatcg atttggcatc
     6781 aactggcaga ttaaccaaga gtgacagcta aagatgaata ttttctggaa acatcaagca
     6841 gcaaacggat catcgtttgt ttgcttgatg tttttgtgtc tatttcgact atacggtatc
     6901 ttttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc aaaaaattaa
     6961 gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca caagcaaaag
     7021 cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa atcagcgatc
     7081 cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta gatatggttg
     7141 ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg cctattttcc
     7201 cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg atcgtaaaac
     7261 gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga gaaggtgaag
     7321 ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag aactatttac
     7381 gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag aatctccaac
     7441 ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac gctgattggc
     7501 tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa gggattgatg
     7561 gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat cagctgcaag
     7621 aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta attacgacaa
     7681 acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc caagaagagt
     7741 taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga cggattcttg
     7801 aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta tctagtttgc
     7861 agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat gctgctcaag
     7921 gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac attacagaaa
     7981 atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta ggaatagaag
     8041 aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa aaagttcaag
     8101 aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt gcgaagcttt
     8161 ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct atgtttgctg
     8221 cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta aagctaaaaa
     8281 gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc aagtcaaaaa
     8341 agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa gtagaactga
     8401 aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg ttgatcacag
     8461 caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt ctcaaagaag
     8521 tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt aagcagaagc
     8581 attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc actctttgta
     8641 aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc tgggaagggt
     8701 acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag aatatcaaaa
     8761 aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta gacttttcag
     8821 atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat caattaggaa
     8881 gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct acatgcttga
     8941 atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg actaaaacga
     9001 atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt tttactggta
     9061 aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc ggtttttctt
     9121 ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa acatcaagaa
     9181 aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat atttattccg
     9241 aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa gcaaaaacgt
     9301 tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa aagtgcattc
     9361 atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag catttctaaa
     9421 gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag catattcacc
     9481 aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt tttttcataa
     9541 aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg tggtagcggc
     9601 tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat attttggtta
     9661 agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt cattttttcg
     9721 taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg gatataatgt
     9781 acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta
     9841 taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt
     9901 catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg
     9961 aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca
    10021 atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt
    10081 atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat cggtgtaaaa
    10141 gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt caatcaagaa
    10201 ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc taaaaaacat
    10261 ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt tcttcaattg
    10321 aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat aatgattacc
    10381 actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt acgacaaacc
    10441 gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg tcattaagga
    10501 atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa atatggaaaa
    10561 tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa tagtaggaaa
    10621 tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata atatatatta
    10681 tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc aaaaatcgtc
    10741 atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt ttttcttact
    10801 ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa tcaaatatga
    10861 acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa ttaaccacag
    10921 ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat atgttcatat
    10981 gtacggatac attttcggac cgattaattt taaccattct atttatttgc ttacctatta
    11041 gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt a
//
LOCUS       DACETN010000087        11028 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DACETN010000087 DACETN010000000
VERSION     DACETN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.090"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..997
                     /locus_tag="IXF98_002143"
     CDS             <1..997
                     /locus_tag="IXF98_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1556444.1"
                     /translation="TNRYHLPFSRKLTNKINIKKIFSPSEPVWKTFEDIEYTDQLSDL
                     WDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAEELFALAKEKGLF
                     IQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENTHEFSTANSYLYG
                     HACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMKISIKSSYFRIKE
                     RPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGVLTYIDEQGSYHE
                     EKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIKSIQESEGKI"
     gene            1086..1391
                     /locus_tag="IXF98_002144"
     CDS             1086..1391
                     /locus_tag="IXF98_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1556445.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            1570..3585
                     /locus_tag="IXF98_002145"
     CDS             1570..3585
                     /locus_tag="IXF98_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1556446.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            3587..3871
                     /locus_tag="IXF98_002146"
     CDS             3587..3871
                     /locus_tag="IXF98_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1556447.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            3897..5270
                     /locus_tag="IXF98_002147"
     CDS             3897..5270
                     /locus_tag="IXF98_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1556448.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5353..7368)
                     /locus_tag="IXF98_002148"
     CDS             complement(5353..7368)
                     /locus_tag="IXF98_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1556449.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7390..8604)
                     /locus_tag="IXF98_002149"
     CDS             complement(7390..8604)
                     /locus_tag="IXF98_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1556450.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            8825..9769
                     /locus_tag="IXF98_002150"
     CDS             8825..9769
                     /locus_tag="IXF98_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1556451.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            10065..11009
                     /locus_tag="IXF98_002151"
     CDS             10065..11009
                     /locus_tag="IXF98_002151"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1556452.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         3626 a         1975 c         2120 g         3307 t
ORIGIN      
        1 tacaaatcgc tatcatttgc ctttttcaag aaaacttacg aacaaaatca atatcaaaaa
       61 aattttttcc ccatctgaac cagtgtggaa aacattcgaa gacatcgaat atactgatca
      121 gctgtctgat ctttgggatg atcctgagat ccaattagtt gtcatcacta cacctagtca
      181 atttcattat gaatatggaa aaatggcact cgaacatggt aagaatgtac tagtagagaa
      241 accttttgca gaaacgtcaa aggaagcaga agagttattt gcattagcaa aagaaaaagg
      301 tctatttatc caatgttacc aaaacagacg gttcgattct gacttcttga cagtccaaaa
      361 agtaatcgaa agcggaaaat taggggatat tttagaagta gaaatgcatt atgattattt
      421 cagaccggaa gtaccagaaa atacgcatga attttctact gcgaacagct atttatatgg
      481 acatgcttgt catacagtag atcaagttct ttcttatttc ggcgaaccag atgatatcag
      541 ctatgatgta cgtcaacttt tagggaaagg acgaatgaat gattacttcg atctggattt
      601 tttctatgga aatatgaaaa tttcgataaa atccagttat ttccgcatca aagaacgtcc
      661 aagtttcgtc gtttacggaa aaaaagggat gttcgtgaaa caaacaaaag atcgacaaga
      721 agaacatctg aaaatgtttt acatgcctga tcatgaagac tttggtatcg atttgcccga
      781 acattatggt gtattgacct atatagatga acaagggagt tatcatgaag aaaaagtaat
      841 ttccgaggtt ggtgattaca gccgtgtcta cgaagggatt tacgagacat tagttaatgg
      901 cgcagaaaaa gtcatcaaag atgaagaaac aatcttacaa atgaaaattt tggaagaagg
      961 aatcaaaagc attcaagaat cagaaggaaa aatatgacat atttgtaaga atcgggtttg
     1021 ttttttgacg cataaactgt cttattgtac gatgaaaaag tatagaacta ctaggaggaa
     1081 ccaacatgac aaattatgac ggtgaaaaac acaaacgatt aagaggttca atggccccta
     1141 aaacagaaga agaagtggct ttagcaggaa ctaattctgg tgaaacagaa gacaaagaag
     1201 aatcattagc tggcacaaac tctggtgaaa cagaaaataa agaaacagct ttatctggtt
     1261 caaattctgt agaagaggaa acaaagccta atgagcccaa tcactttgaa aaagatatcg
     1321 aaaaagcggt tgaagatgtc aaagaaaaat ttgaagaact gacctcaaaa aataaacatg
     1381 atcatcattg atcggacgaa atcaattcat aaaagcagga gacatttggt cacctgcttt
     1441 ttttctgtat ccattcgata gaaatcgttc ggtacagtcg taaaaaaaat ccaactgtaa
     1501 gttgtaccaa acacccctcg atttaaaagc gctaccatac tagaatataa gtaggaggtg
     1561 aacagcgctg tgaactacaa aattagttat ctcctgaaaa caccaagaga aatcagtcta
     1621 gaaaaagcag cagagatcat cgaagtaaat acaagcgagg cgatcaaaca attaactcct
     1681 tttgccgaat atgtggatga tacccgtatc cgtataaaga aattccgagc aatcgactgg
     1741 gcagaaaaat ttttggatat tactcgattt gaagctgtgt ataccgaaca agagcgaaga
     1801 gatcttcttt atttgatggt ttattcgact gcaacgtctg tttctgtttt tcattttcaa
     1861 gattttcttc aggtttctaa agggacagtc ctgtcagata tcaaaaagat aagaaatgaa
     1921 ttagaagtgg aagattgtca gctgtcgtat actaggaaaa ctggttttag gctgctaggg
     1981 tcagaagcaa tattaagaag agaagcaaaa aatcgcactg ctcgtttgat gcagtcactt
     2041 gctggaagat ttgggctcac gtatttaacc gcaacccttt ccattgatta ttacgccaaa
     2101 gttagagatt tgctagataa aacgatttca ctatcagatc ttcgattcgc accaaatcga
     2161 atggatgaat tagcttatta tttgagtttg acaaccattc gaattggaaa aacatccatg
     2221 gaactggtaa aagatgctga actgttggct tctcttgcag tcactgacat tagtcgacaa
     2281 gtattgactg gccttggcta ttccgaacat caaaattcgg agattatatt tttctctctt
     2341 tgcttagcat cgatcgttga aggagatgtc caggagcctg cacttgattt tttgctggaa
     2401 tgttcttcag aagtcatcca tcgcatggaa ttcctgatgg ccgtagaatt tgcttcgttt
     2461 cgtgaattat tgatgaatgt cttttatcac ttggtccctg cctattttcg tatttcctat
     2521 tctttttatt tacctaatgt gatgatcgat caaattaagc atcaatatgc ttccatctac
     2581 gaaatgacaa gaaaagcatt acttccgtta gaaagacgaa tagggaagag catccctgaa
     2641 gaagagattg gttttttcac tatactattt ggcggagaga tcagaaaagc ggacgcagaa
     2701 gaaagaaata gaaaaataag agccgtgatc gtctgtccaa gtggcattag ctcttcattg
     2761 atcttaaaaa gtgaacttca gcaattattc ccgatgatct tttttacaga gactaattca
     2821 gctgaccgct tacacgaggt tgacgaaact tcttacgata ttattttttc tactgtacct
     2881 ctcacactta ctgataagaa aaagtctctc tatgttctgc atccaatcat gactgacttg
     2941 gaaaaaaatc agctgatcaa tcaagtacaa agagattggc tgataccagg attttcaatg
     3001 ccttctgccc aagaattgtt gtcggcgcta ctgccttata tcgaattaaa agaaggcgta
     3061 gacgaagaaa agctctatca agtattaaat cgtaaaatga acaaactttt agaaaagaag
     3121 gaggatcaac gtcctatgct atctgaatta ttaacagaag aaatgatcca attatcggat
     3181 caaccaaaaa actggcaaga agcaatcaca tttgccgcac agccgctatt atcagaaaat
     3241 aaaatagaag aaagttatat caaagctatg atcgagcgcg tggagcagta tggtgcgttt
     3301 atccatattg gcgatcacat cgcattacct catgctcgtc cagaagatgg cgtaaacgaa
     3361 gtagggatgt ctttgctgaa acttcaacaa cctgtggatc tgcttgataa tccagcccat
     3421 cccattagtt tgttcatctg tttagctgct atcgataatg aggcacatct tcgggctttg
     3481 gcaaatttaa caaagctact ttctaataaa caaaatcttc aagatttatt acacgcatca
     3541 acaaaagaag aaatattagc gatcataaga aaaggagacg aatgatatga aaatttttgc
     3601 agtatgtcaa agtggattag gaacaagttt tatggtagag atgaacatca aacaagcact
     3661 tgcagatctt ggagcagatg tggacgagtt tgaagtaagt catacggatg ttggaagtgt
     3721 atctagcgat atggctgatt atttcttcat tgagcattct ttgaaagata cgctaacttc
     3781 tatacctgat gaaaagctcg tacctttgca atcgatcatt gacagcgaag aagtaaaaga
     3841 aaaagtaacg aagatcttgt caaaagaata aaaatgattc aagggaggaa ctatcaatgg
     3901 ataaattgtt gaatttgttt gtgtcaatcg tcagtcagcc ctccatcctc gtttctttga
     3961 ttgcactgct tggtctcgtt cttcagaaga agaaattttc agaagtgatc caaggaacta
     4021 tcaaaacatt tgtcggattc ctagtactga tcggcggtgc tggtttaatc agtaattcac
     4081 taaccccttt tgcttcgatg ttccaaacag ctctgcatac tcaaggagta gttcctagca
     4141 atgaagcagt agtggcgatt gctttgcaac aatacggtac acctactgct ttgatcatgc
     4201 tagtagggat gatcgtgaat attttacttg ctagattcac aagatataaa ttcatttttc
     4261 ttacaggtca agcaatgctt tatgtttctt gtctaacagc agtcatttta gtaagcgcgg
     4321 ggtttactgc aaatttgtgg atgatcttat taggcggggt cttcgaagga acactattaa
     4381 ctattacgcc tgctctttgt caaccgttta tgaagaacat cactggaaac aatcaagttg
     4441 cgttagggca taccggaaat atcggttatg cttctagtgg atggatcgga aagttttttg
     4501 gaaacaaaga ccgttctaca gaggacataa agattcctaa aagtttcagc tttttgcgag
     4561 attctactgt atcgatcatg ctcttgatgt cgatcgtata tgtcgcactt gctttgttag
     4621 ctggtcctgg atttgttgaa agagaactaa gcaatggaga caacgctgta atctttgcac
     4681 tgatccaagc tggaacattc acagctgggt ttgttatcgt ccttcaaggg gttcgaatgg
     4741 tgttggcaga aatcgttcca gcattccaag ggattgctaa aaaactcgta cccgattcta
     4801 aaccagcatt agatgttcct atcgtttttc catatgcacc taatgctgta ttaattggct
     4861 tttttgttag cttctttgta ggaacactat ccatgtttgc tatgataggc ttgcaaacta
     4921 cagttatcat tcctggggta gtaggacatt tcttctgcgg agctgcttct ggtgtcttcg
     4981 gcaactcgac tggcggtaga agaggggcgg tattaggttc tgctttcaat agcttattga
     5041 ttagttggct gccattattt atccttccag tattaggaga tcttaaaatc gcttcttcta
     5101 catttgccga tactgattat ctagtaccag gacttgtact tgggaaacta ggagaatacg
     5161 gtcaaatagc cttgacagtt ggaatcatcg ctttcttttt acttgtcgta ttatctagct
     5221 ttctttttgc cggaaaaaga acaaacattg tttctaaaga acagaattaa gcaaacttca
     5281 aaataaaaat tgcttggaca atatcagaag aagatttaaa gtcttctgat atgtcgcaag
     5341 cagttttttg ttttatgcat ttatttttga atagcttgtt aagacaagtg gctgttttgc
     5401 tagaagagga taacttttac cattcatttt cactgttccg cctgttctat cagaagtaat
     5461 cacgatctta tcttttgttt tttcaagcga aatctttcca tctttgatag gtagcgtaca
     5521 attcagtgtt tcaaatgaat ctagattagg ttgtatcata aattcttcat aacctggctt
     5581 tgttggatac aaaccgataa aatagcgtcc taaaagatag attgggcttg ctccccatgc
     5641 atggcataag ctttttccat aaggatctcc atacatactg tacttttcat ttcctgtttg
     5701 agtagggtcg tattcttccc agaaagttac cgcatcatga gataacatcc ctccccaata
     5761 atcaagaatc gtttggtaga cgatattcgt ctgtccgagt ttgcataaag catcttgttc
     5821 atagaatttg aaatatggag tagtaatctg tgtgatagtg tcattcaata agacattctt
     5881 taagatcgat tgctgttggt ctttgttagc aaaatcgaat aaaacagcga aaatattcgc
     5941 atgacgcgtc acatttcttc tcccagattc atagcaatcg ataaaagcgc caagttcttc
     6001 atcccaaaag tagtcaaata ggttttgttt caatagttga tatttttctt gatagagatg
     6061 agtatgataa ttttcgttct ctccatatgt ttcagctaag atatctccgc aagtgatcat
     6121 ggaatgatag gctttcagta ataagacttg ttctgcagca agcgttcctt ctttatccat
     6181 ttcagaccaa tcaataaata tccaatcttt tgctctccca taaataaacc ctaattcatt
     6241 cgtttgctcc atcaagtatc tcattaatga ttgcatctta gggtagacga tgcgtaaaaa
     6301 ttcatagtct ttcgtcatca tatagtggtt ttcgatacca atcaaccaca agatcgaata
     6361 gtcaacgata gtattcatat gctgcttgat ttcattttgt cctcttaatg ctaatatcgt
     6421 tcgtttattg atttcctcgt caaaaaaaag atattgattg atcatgaatg attgatacgc
     6481 atctccagac caaatccagc gatctcgttt gataccgtcg ataaaaaaca aatcactaca
     6541 taacttgaag gcttcttctg atactgacca gatctgattc agtaaagaag aatcgcttga
     6601 aaaagcagct tgtttcgaaa gagataaaaa ttcatgaaaa acttgaagtt gaaccattcc
     6661 agtagaaacc tctggtataa acaaatagcg aaaagcgcgt ttacgagctt tcatttttct
     6721 tgtcattcct atttgcttat agtagcaata atcgatatcc atagcttcta catcggactc
     6781 tccataacat agagtgatag attcgatatg gtcatccaga gatattagtt ctacacagcc
     6841 attcactgct cgcccaaaat cgaataagat tcctccgcca atctccgttt cagtgactgg
     6901 atggacgcat tctttttgat aatagatttg atttggatct tttgcgatat cgacataaag
     6961 cggactccag ccagctggat attcttcaat aaaattactt gctgtccagc caatatctga
     7021 ttttacttcg tctccctcga taaacatcgc tggtagccct gatgtatgtc ctacaaagat
     7081 acgaattttt gctttaccag ctggacaagt cagccatttt ccacatggat gtttttgtcc
     7141 attaatttct acatacccaa ctccttggat cgtcacttta aacatggaag gttgatcaag
     7201 aaagtaatat ctcttaaact tgacgttccg atggcaatca tccatgtacc agtacgctgg
     7261 ccagtcaaaa cctcgttctt cccgttggaa attttgtagc agtccatggc ggatctcaaa
     7321 gtctccagga taccaaagcc atctatcttt atgtttttca tttgtcattt tacacgacct
     7381 ccatcaaatt tattttgttg tcaattgttc ttttatttct tcatctactc ttatcactgt
     7441 ctttctattc gtcctgtgta ctactagaag gctaataagc gcgctaatca acagcaagat
     7501 actgccattg acgaaaacag caagtgtttt actcccaaga gaagcaaaag agagaaagag
     7561 caaaccatat ggtgcaatga aagtacctaa attagcgcca accaataaga ttgaagtatt
     7621 gaaactctcc gcgtacttgg gcgcttggat acttacttgg ttgaataagt aaggaatgaa
     7681 taacgaaaaa gaaattcctg ttaggatcgc accaaaccca atcacataca ggtttatcac
     7741 agtggcaatc aatacaaagc cgattcccat cagtagtaaa gaaagtgtaa gcatatagcg
     7801 atgtgctttt gtctgaagcc agccaaaaca aactccggca gccatacttg ccaattgcat
     7861 gattgttaag atattcgctg cattagtttc gtttcctaga gaataagtcg tcaggactgt
     7921 ggctaattta atcgatacag cattatatac cacgatgacc agcaagagaa aaataccata
     7981 actaatcatt ttaggattcg ttttgattac ttgtcttgtt ggggctgtgc catgcgattg
     8041 aagttcagtt gtttcaggtg taggaacaaa caaaacaaac aagacaagaa taggtaagct
     8101 gatggcgtag actaaaaagg tatattgcca accgaactgt actagctgtc ctgcaacaaa
     8161 tgtcattaaa gctgcaccaa atccttgaaa tgcactttga aaaccaatca tagaggctcg
     8221 ttcatctccg tgataaaagg cagcaatcat gctgactgcc aaagaattga tcaatccaaa
     8281 gccaatacct agtccagctc ttgaagctaa gacaatactg taactttgtg agaagagggg
     8341 gaggatacca caaatcgatg caatcactaa tccgatgatc acagtttgtt tttgtccgat
     8401 tttcctagca ataaaactac taatcaaaac aaaaatagtg accataaaag agggagcagt
     8461 cgttaataat tcaacagcag ataaagaacg cgtggcataa gtagctgaca tactgggaat
     8521 tgcagacgtg actgcaccag cagaagtcaa aataagagag actgacagca gagaaaattt
     8581 catcatcttt ttattcataa gcatgttcct ttcatttttg taagcgctta tcaaaaatat
     8641 agtatgaagt ggttattctc tcaatagcgg aatcacagga agttcatgtc ccaaccatag
     8701 aaagtcttta tgaaaccaaa aatagctttg catataatag cagtacagcg tgaactaata
     8761 agcgaaagaa accgcttaca aaaaggaggg agtcaattgg aactagctga attagacatt
     8821 ttttttgcgt aacgaagaat caatcgaaac tctgcagcgt gaacaaggaa tgaatatcaa
     8881 tgatttaaac ttagcttacc atcttcagac accgccaaaa atccaccgag gggatctatt
     8941 tcaacaggga aatattcata tcagtaaaca ccgtcgatat gcggaagttc ccgagcatac
     9001 acatgatttt gttgaaatca attatatgta tagcggtaca tgtttacaaa ttattaatgg
     9061 agaaaggata gagatggaag aaagtgacgt ggtgatcatt gataaagaag ctactcaaag
     9121 aatcgaatac acagggcaag aggatatttt aatcaacatc ttattaaaag aagaaacctt
     9181 atctacagag atattgaatc atttggcaag cacaaataat ctactttctt cttttttgat
     9241 aaatgcatca aaagaaaaag gggaacatac acaattcatt cattttcgcg gcggaaaaac
     9301 aagtcgtttg acgcatttga ttgaaaatat gctgttgatt tattttgata catacagtca
     9361 taagatccgt tcgttaaatc tctatacatc actattactg atcgaattga ctcatttatt
     9421 agaacaacaa agcttttcat cagcagatgg atgcgcagaa aatgagatgc ttagagtatt
     9481 agattatatc gataagcatt ttacttcttg tacgttaaaa gaaacagcag ctcattttgg
     9541 ctacaatccg gtctatctta gtaataaatt gaaaatgtat tttgatgctt cctttcaaga
     9601 actggttcta aagaaaaaat tcgactttgc gttggaactg attcaagaaa cgagtttttc
     9661 gttggaagaa atcgctaatc gtatcggata taaacaaacg acttctttat tcaaattatt
     9721 caagaagtat tcaacatgta ctcccaatga atggagaaat aaacattaag aagaaaatct
     9781 ttcactaaag catatgaagg aaaattgttt taggtgcgat tgctgtctta ttatatcgtt
     9841 gcagcgaatt tttttggatt tattttcagt tctaaacttt tggttgtatt atcaggggat
     9901 ataggtgtaa acatttgtca tttatctgtt atgcgtattc aggaattcgt ttccatcaaa
     9961 aattataata acaactatta taattaacat gttgataaaa tgaataaaca agactatact
    10021 tttcatgaaa gtaacaaata gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc
    10081 gtaaagtagt aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca
    10141 accaaggaat ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg
    10201 aagcattgga ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag
    10261 gtggctatga agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa
    10321 aacctggtca atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg
    10381 taaaagaaat catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag
    10441 atattttgac atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta
    10501 ctggaacaac attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg
    10561 acccacgcag cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat
    10621 ggtcacatac aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta
    10681 ttgcccaaga tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca
    10741 ttgatcgtaa aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag
    10801 ctatcttgaa taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg
    10861 acgaaaaaga tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag
    10921 ttgttgaatt atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt
    10981 taagagaagt attgaacact gtattataag aaacaatgaa tatgaact
//
LOCUS       DACETN010000088        10767 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DACETN010000088 DACETN010000000
VERSION     DACETN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.091"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            79..1125
                     /locus_tag="IXF98_002152"
     CDS             79..1125
                     /locus_tag="IXF98_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1556453.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IXF98_002153"
     CDS             1474..2106
                     /locus_tag="IXF98_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1556454.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IXF98_002154"
     CDS             2694..4133
                     /locus_tag="IXF98_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1556455.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IXF98_002155"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IXF98_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IXF98_002156"
     CDS             4895..5110
                     /locus_tag="IXF98_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556456.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IXF98_002157"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IXF98_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IXF98_002158"
     CDS             complement(5770..6681)
                     /locus_tag="IXF98_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1556457.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IXF98_002159"
     CDS             complement(6700..7704)
                     /locus_tag="IXF98_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1556458.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IXF98_002160"
     CDS             complement(7701..9824)
                     /locus_tag="IXF98_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1556459.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IXF98_002161"
     CDS             complement(9829..10749)
                     /locus_tag="IXF98_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1556460.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACETN010000089        10705 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DACETN010000089 DACETN010000000
VERSION     DACETN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.092"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(85..801)
                     /locus_tag="IXF98_002162"
     CDS             complement(85..801)
                     /locus_tag="IXF98_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1556461.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(925..1110)
                     /locus_tag="IXF98_002163"
     CDS             complement(925..1110)
                     /locus_tag="IXF98_002163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556462.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1337..2610
                     /locus_tag="IXF98_002164"
                     /pseudo
     CDS             1337..2610
                     /locus_tag="IXF98_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2717..3748)
                     /locus_tag="IXF98_002165"
     CDS             complement(2717..3748)
                     /locus_tag="IXF98_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556463.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3755..4585)
                     /locus_tag="IXF98_002166"
     CDS             complement(3755..4585)
                     /locus_tag="IXF98_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556464.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4582..5388)
                     /locus_tag="IXF98_002167"
     CDS             complement(4582..5388)
                     /locus_tag="IXF98_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1556465.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5394..6218)
                     /locus_tag="IXF98_002168"
     CDS             complement(5394..6218)
                     /locus_tag="IXF98_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1556466.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6208..7308)
                     /locus_tag="IXF98_002169"
     CDS             complement(6208..7308)
                     /locus_tag="IXF98_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1556467.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7486..8271
                     /locus_tag="IXF98_002170"
     CDS             7486..8271
                     /locus_tag="IXF98_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1556468.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8304..8687
                     /locus_tag="IXF98_002171"
     CDS             8304..8687
                     /locus_tag="IXF98_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1556469.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8763..8894
                     /locus_tag="IXF98_002172"
     CDS             8763..8894
                     /locus_tag="IXF98_002172"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1556470.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            8863..9099
                     /locus_tag="IXF98_002173"
     CDS             8863..9099
                     /locus_tag="IXF98_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1556471.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9177..10148)
                     /locus_tag="IXF98_002174"
     CDS             complement(9177..10148)
                     /locus_tag="IXF98_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1556472.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3170 a         2500 c         2045 g         2990 t
ORIGIN      
        1 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
       61 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      121 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      181 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      241 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      301 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      361 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      421 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      481 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      541 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      601 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      661 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      721 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
      781 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
      841 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
      901 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
      961 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1021 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1081 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1141 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1201 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1261 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1321 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1381 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1441 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1501 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1561 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1621 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1681 ttataaaata ttggaggaat tggctgaaaa acaagcaatg acgacgattt ctaaacattg
     1741 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     1801 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     1861 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     1921 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     1981 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2041 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2101 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2161 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2221 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2281 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2341 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2401 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2461 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2521 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2581 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2641 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2701 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     2761 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     2821 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     2881 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     2941 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3001 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3061 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3121 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3181 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3241 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3301 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3361 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3421 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3481 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3541 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3601 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3661 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3721 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     3781 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     3841 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     3901 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     3961 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4021 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4081 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4141 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4201 acactacaaa cattgggata ccagttttag catacgttcg aagcaaagcc gttaccaaaa
     4261 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4321 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4381 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4441 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4501 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4561 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4621 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4681 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4741 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     4801 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     4861 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     4921 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     4981 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5041 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5101 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5161 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5221 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5281 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5341 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5401 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5461 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5521 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5581 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5641 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5701 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     5761 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     5821 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     5881 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     5941 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6001 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6061 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6121 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6181 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6241 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6301 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6361 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6421 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6481 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6541 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6601 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6661 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6721 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     6781 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     6841 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     6901 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     6961 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7021 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7081 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7141 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7201 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7261 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7321 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7381 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7441 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7501 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7561 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7621 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7681 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7741 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     7801 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     7861 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     7921 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     7981 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8041 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8101 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8161 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8221 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8281 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8341 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8401 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8461 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8521 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8581 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8641 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8701 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     8761 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     8821 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     8881 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     8941 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9001 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9061 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9121 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9181 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9241 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9301 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
     9361 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
     9421 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
     9481 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
     9541 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
     9601 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
     9661 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
     9721 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
     9781 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
     9841 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
     9901 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
     9961 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    10021 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    10081 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    10141 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    10201 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    10261 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    10321 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    10381 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    10441 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    10501 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    10561 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    10621 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    10681 tgtcattaca gcaaaaaatc acgtt
//
LOCUS       DACETN010000090        10408 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DACETN010000090 DACETN010000000
VERSION     DACETN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.093"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..71)
                     /locus_tag="IXF98_002175"
     CDS             complement(<1..71)
                     /locus_tag="IXF98_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1556473.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEA"
     gene            295..1086
                     /locus_tag="IXF98_002176"
     CDS             295..1086
                     /locus_tag="IXF98_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1556474.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1052..2005
                     /locus_tag="IXF98_002177"
     CDS             1052..2005
                     /locus_tag="IXF98_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ1556475.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2403..3377)
                     /locus_tag="IXF98_002178"
     CDS             complement(2403..3377)
                     /locus_tag="IXF98_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1556476.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3492..3926
                     /locus_tag="IXF98_002179"
     CDS             3492..3926
                     /locus_tag="IXF98_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1556477.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4051..5071)
                     /locus_tag="IXF98_002180"
                     /pseudo
     CDS             complement(4051..5071)
                     /locus_tag="IXF98_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            5463..5885
                     /gene="arsC"
                     /locus_tag="IXF98_002181"
     CDS             5463..5885
                     /gene="arsC"
                     /locus_tag="IXF98_002181"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ1556478.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6165..8087)
                     /locus_tag="IXF98_002182"
     CDS             complement(6165..8087)
                     /locus_tag="IXF98_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ1556479.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8174..10114)
                     /locus_tag="IXF98_002183"
     CDS             complement(8174..10114)
                     /locus_tag="IXF98_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1556480.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
BASE COUNT         3194 a         2031 c         1783 g         3400 t
ORIGIN      
        1 gcttcttctt tattttttac ttctaccgct aattgaatca aactaggaac gaacatctct
       61 ttatatttca ttccaacctc ctcctttttg attaagtttg ttaatttatt gttctactaa
      121 gttcatcttt gttttacaag aacattatat ttctgttagt ttgttttgtc aacgctttca
      181 aaagaaattt attttgttaa atttgatcta cttgaaaata aaatcgttta tctatataat
      241 taagaagaac aaagtcgaac aaaaattgac ttttatgtta aaggagtaat tcatatgctg
      301 aaaaaagaaa gacaagaaca aatcattaca ttattagaga aagattttta tttaactaat
      361 tcagaaatcg ctaacaaatt aggcgtatca gaaatgacca tacgccgaga tgtcactgaa
      421 ttagcaaaaa aaaataaagt aaaaaagcta tatggtggcg ttgaacgaaa ggaaccttat
      481 aatcgagaac tatcgactca agaaaagatt gatacgaatg tagccgaaaa aaaatatatt
      541 ggtcaagtca tgaacagtat gattcccgat aattctacta tttatgtcgg tgctggaaca
      601 acgatcctat atgcacttcc tttcattcaa aagaaaaatc tatttgtaat tacaaacagt
      661 ttgattgctt tcaactattt aaagaatcat acaagttatc ggattttact tactggtggc
      721 gaattttctc ctattacaga agagttcatt ggggaagttg cagaaagatc ttttgaaagt
      781 ttgaatgtcg atatctcttt cgctgcaaca aacggcatat ttgataacaa tgtgacaacc
      841 gctcaattta ttgagggaaa tattcagaac gcagcgctca atgcagcaaa aatcaaatgc
      901 attgttgcag attattcaaa attaaataaa tcagacgtct atacttttag aaaattaacc
      961 gattttgatt atctaataac cgataatcat ttatcagaac aggatttcaa tcattattca
     1021 aaatacttaa caatattaaa ggagcccaaa aatgatactg acaatcacga tgaacccatc
     1081 cattgattcc atttatttta caaaacactt tgaattaggt acgatgacac gctttgattc
     1141 tccggtacgc tatgtaggcg gcaagggaat caattgcgga cgagcttctg ctctactagg
     1201 ttcatcagtt attttaacag ggtttttggg cggttatttt ggcagtatga ttcaagatat
     1261 gctgaaaaat gaaaatttgt ttgatttgca ttttttacct attgcagaag aatcaagagg
     1321 tgccgtcacg atcatgcatg atgatgaaac gcagacagag ctagtggaag ctggtcctga
     1381 aatagcagat actactatca aaaaattaat gaatgatatc aaactaatca gtaaaaataa
     1441 ttacctttca gtcattacaa taaatggttc tgttaacaat ccagatccct ttttgtatgc
     1501 taaattatta caatatatca aagaacaaat agatccttcc acctatatat taatggatat
     1561 atctggacaa catctccgtg aagtcttagc aagtaagtat aaaccgatgt tcatcaaacc
     1621 taatatccat gagttttcag atatcatcca tcaaaaaatt actcaaaaaa gcgacataaa
     1681 acaccatcta aaaacaaatc cactatttaa agatatcgat gttatcatga tttcttgcgg
     1741 ctcgtatggt gcagtagtca aagtaaaaga agaattttgg aatgttgaga tccctaagat
     1801 tcctatcatt aatacaacag gctctgggga tgccactgtt ggaggttttg ctcattcatt
     1861 agaacagaat tatcctttaa aagaatgtgt aaggtacgca atggcttgcg gcatgtcgaa
     1921 tgcacagcaa tcagaagtcg ggaaagttat tccagctgat gtaaaaaatt tagcaaaaag
     1981 cattaaaatc acagaaatga aataaatagt acaaagtaaa taattgttta tatgaaatga
     2041 gacacaaaga gactgtgaca aaaaacgtgt gtaccgaccc ccaaaagtta gattttttgg
     2101 tctaactttt gggagtcgat acattgtcac agtccttttt tcgaataaac ggtgttctcc
     2161 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagcta ttttttgaaa
     2221 ttgctccttg tgactcagag ttaaacggca ggctcacgac ctcttggtat aaacggtgtt
     2281 caaaagcttg catctacgct aatgagtcca accaagtcaa aaatgaggag cttttttggc
     2341 tgtccttatt acttgatgca gggcagtttt ttcataatct cttttagatt aaatcctttt
     2401 ggttacacaa ggttctcttc aaagaatgtt gccatttttt caaatggaat gatgtctgtc
     2461 tggtcgtata gatctgtatg ggatgctcct ggaatgatca tgagttcctt gttgtcacct
     2521 tttagttctt tgaaagcgtc ctccccaaag taacgtgaat gagctttttc cccgtgaatg
     2581 actaagactg catgacggat ttctggaata taatgcaata gttttgcatt cataagtgac
     2641 aaccctgatg tcctattcca accatcattg gagtttaaag aacggacatg atatccgcgg
     2701 tctgttttat aatacgcatg atagtctttg acaaattgag gagcatcttc aggcaacgga
     2761 tcaaccacac ctccagcacg tgcgtagtcc ccttgtttga aatcaatgat gcgttgtgca
     2821 tttaattctt cacgcatttc atatcgggca ttttcatcca taacatcgaa gtagccattt
     2881 gctgttactc gagtcatgtc atacattgtg gaaacaactg ttgctttgac ccgcgtatcg
     2941 atagctgctg cattcaatgc catacctccc catccacaaa taccaatgat tccgattctc
     3001 tcagggttta catttttttg tacagataaa aagtctactg ccgcttggaa atcttccgta
     3061 ttgatatcag gcgaagcaac atatcgaggt actcctccgc tttctcctgt aaacgatgga
     3121 tcaaacgcaa tcgttaaaaa gcctctttca gccatcgtct gcgcatatag accagacgat
     3181 tgttccttca ctgcaccaaa tggcccgctc acagcgattg ccggcaattt cttttcactt
     3241 acttcttttg gttcatataa atcagcgact aatgtgatac caaaacgatt cgggaagctc
     3301 acctttcgat gattgacttg attgcttttt ggaaatactt tgtcccattc ttgtgtgatg
     3361 tttaattctt tattcatatt ctagactctc ctttgatcaa aattgacatt tgtttttgaa
     3421 tgtattatta tgataaaact taaacttgac tttaggtcaa ggtaaaaatt ttattttagg
     3481 aggatttttt tatgtattcc attggacaag tgtctgaaat gtttggattg cctgtttcta
     3541 ctttacgtta ttacgacaaa gagggattat ttcctgatat ccagcgtgtt tctggtatta
     3601 gaaaattttc agagaatgag ttagaggctt taagagtcat tgactgtttg aaaaaatctg
     3661 gtttagaaat aaaagacatt aagcaattca tggaatggag caaagaaggc agtaagacat
     3721 ttgaggttcg taaagaacta tttgaaaaac aaaaagaagt agtagaaaaa gaaatagcaa
     3781 aattagaaag agtactcgct atgctaaact acaaaagctg gtactatgaa gaagcaataa
     3841 aagccggcaa tgaagaagcc gtattgacaa tgattcctga tgatttaccc caacatgtaa
     3901 aagaagctta tgtacattct cattgaacaa acggataact cactctcctt ctctatgaac
     3961 ttcatgaaaa catgaataaa cacctctaac tgattgttag aagtgtttat tttttcactc
     4021 aagtatcctg cacttacaaa aatatccttt ttaccgccat cctaattctg gagcgatatc
     4081 ttttacgatt gtttcaagca agtgcatgtt atattctacc cccaaagtat taggaatcgt
     4141 gacaagtact gtgtctgctt ctttgattgc ttcatcttca gctagctcct tgattaattg
     4201 atcaggttca ccagcaaaag tttttccaaa aatcgttggg tttcggtgat aagcaagata
     4261 gccgacagca tcttgttgcg gactaccgtc ttgtccaaat aagcgtcgat ccaaatcagt
     4321 ggtgattggc tgaatcgagc gagtaactaa tgttcttggt tcaaaatcat gacctgcttg
     4381 cttccacgct tctttatacg cacgtaattg gtttgcttgt tggatatgga atggctcgtg
     4441 ggattcgtaa ttgactaatg tagatgattg cagattcatt ccatgttgtg ctgcccagat
     4501 tgccgtttgc tgcgaaccag ctccccacca gattctttgt cgtaatcctt ctgaataagg
     4561 ttctatccgt aattttcctg atccttgtgg gaacattggt tgaggatttg gttcttcaaa
     4621 tggttttcct tcaagtaatt ccaagaattc aagggtgcgt tcccgtgtta cctctttgat
     4681 ttcttcttca gtaatcataa ccaaagtatt tccagccatc tagaacttgt tcaggtgaac
     4741 cacgccccac tcctagttgc actcttcctt gcgtgatgat atctgtcaat ccggcatttt
     4801 ctgccatata ataagggttt tcatatcgca tatcaataag tcctgtaccg atttcaatct
     4861 tacttgtctt tgcgccgatt gccgctagca aaggaaacgg cgacccaatc tgaggggcaa
     4921 aatgatgtac gcggaaaaac gctccgtcca ctccgatttt ttctgcttcc accgctaaat
     4981 caatcgattg caaataagca tcttgagctg atttcacaag cgatccttgc tccatccaat
     5041 gtccaaagct taaaaagccg atttttttca aggtaatgac ctcctttaaa cctacaaaaa
     5101 tggaaggttt tttattttta atatgaactg attctactac tttttttctt aaaagacgaa
     5161 taattgttct cgcctcacaa tcaagaaatg tatcggctta ctacacccct actttcttca
     5221 tcatctgccg tgtcttaatc tcttttaaca gtctgttaca tcgttgattt ttgactcaaa
     5281 atgattgttt ctaacatgta tttatggtaa aaaagacaag attagttctt tgtacacatc
     5341 gatttatacg ctgaacaaaa agaaatatat aaactaagaa acccattgtt ttttcgctcc
     5401 atcacataga gtttacttta gataaacagt gacatttact aatccataac ggaggaaatc
     5461 aaatgaaaaa aatctatttc ttatgtacgg gcaattcatg ccgcagtcaa atagcagaag
     5521 gctacgcgcg taaatacttg tctccttcaa tgtttgagat aagaagtgcg gggatcgaga
     5581 cacatggctt gaatccaaga gctataaaaa tcatggcaga agacggcatc gatatttcca
     5641 aacagacgtc tgatttgatt gatagagatt actttactca ggcagacttg atcattacac
     5701 tttgtggtga tgccaaagac aagtgccctg ccgttccaaa caatcacgaa caccttcact
     5761 gggacctaaa agatcctgca caagctgtcg ggagcgaaga agaaatatta acggaattca
     5821 gaaaagtcag agaaggaatc aaaagaaaag ttttagactt aaatcaacgt acatcgatcg
     5881 tataaagaag tattcaaaat gatttttcaa gcactaagaa acaatgttaa aaaaatagct
     5941 ttccatattt tacaaaattg tgccttattg ccgacaaatt gtctcttgaa caccgtttac
     6001 cagtagtcaa gacatcgtaa gcaactataa aaaattataa taaactcaaa ataaaaggat
     6061 gtgccacaac tttggccaca tcctcttttt ttgtatttag ttttctactt ccaaataaat
     6121 agatagaatt attataaata taaaggcagt tttttaatcg ctacttattc ataactagat
     6181 aacaacgtta agatttctag cggatgattg gattgcatca actgagccat ttgttctggc
     6241 tgcttttcta gatagtgaat aacagaaaat atttttttga actctgcatg tttcaaatca
     6301 tttttcttta ttgctaatag cagtacgata cgtatctgtt ccccgtgcca taggactggt
     6361 tctttcattg tagcaataga aatcatcgaa tgcttcacat acagcggatt accgtgaggg
     6421 attgttacaa atggtgctac tctagtactg gagtgactgt ctctttccaa gacagaatcg
     6481 atgaattccg gctccacata atgatgttcc actaagacgt ttcccataaa gtttaaaata
     6541 tcggctgggt ctgtccgttc aagttgagga aagatcagaa atggatgagt aaagcttgct
     6601 agatcaaatg tttttttctt cttgataggc acatgctcca gcatatattt tgtcaaatag
     6661 gctagatcag tttctgtgat cattggtgag atctcgatta ctggaatgct tatatgttta
     6721 aatggtaacg tagccaagat aaaatcaaca gagggaaaat cttgtctctt taattctggc
     6781 ttatattcaa ctagatccat aatcgttaat tctggaagtt ctctgttcag ccgtgcttcg
     6841 agaaaagtag ccatagcttt tgaatattca ctaactaaaa ccgtttgata tcttctactt
     6901 ttctgatgtt tgtgtcgctc gatcgctgct tggaaatgga tcgttaaata agctgtctcc
     6961 tcttgtggaa cataaaaatt ttccttttca aaataatctt ctaaaattaa ctgtaaaact
     7021 aagaaaagtt gtgtgtacgt actaaacaca tttttcgtca atggattaga aatctgcaaa
     7081 ccataatgga gtcgaaaata agttgaagat aaatgagacc ttaaattttg ttttagtagc
     7141 tgatcttctt ttaatgaata gcctagaaac tgttccactt gttcgatcaa atgatcagca
     7201 agtgcgctag attccttttg aaaacgtgtg gctgcttgtg aatttgtaaa aagattggcg
     7261 atacgcaaag ccagataatt gatttcatca ggagaaaatt caattggata gatggtggct
     7321 agttttgcta atatttgact acttaacttt tgtgcttggg tgtgttccac gacttcgttt
     7381 tcgtctttac ttaatttcac tttctggtac tgtttcattc gctcgagcat aaagtagatg
     7441 tgaatctcca cactagccag tccattcggc tgttctaaat ggttttcata aaaaacatct
     7501 tgtaatgtat gttggataac ttttaatgta tctttagcga agaattcttt caacgacgga
     7561 ctttgtggat cttctttctg catatttcgt aatgtcttaa caagcaactg tcttttttct
     7621 tgttcatcgc ctttaagaaa agttcctagt tttgttttgg atactagctg taaatggtga
     7681 tactcaaaaa agctttgcaa ctctgtcaat tctttacgaa tcgtacgttt tccaacatat
     7741 agtttttctg aaagttcttc aagactaaat ccgtcttttg ctactagcag atggaaaatc
     7801 aataactgac gaagctgttt tttatccatt tctgtttgag tctcttcatt aagcgctcgt
     7861 ttcagttgtt ccctttcgtg aggcagacca ttcaacaaga tacctgtacc tttttttctt
     7921 tcaaacatta aaggcagatg tttgtctctc aaccaagttt caatggtatt caaatcgcta
     7981 tgaatcgttc tttctgacac atggaaatgc tctgctagct tttttgttgt tatataatta
     8041 ttgttctcac ttaattgata gatcaaagat tgctgacgct gattcataat gtgcagaaac
     8101 tcccttctaa ataaaaaatc gatcatgata acacatctat tatgtctcat cacggtcgat
     8161 ttttcatctt atgttacatt tcgcttaata attggtgaac tgttgtttta tctggcgctt
     8221 ccatcagtgc ttgacggaag tcatcatcca tcaatttacg tgatagacgt tgcagtattt
     8281 tcaagtgcat atcaccttgg ctgttttcag gtacagcaat cataaagatc atatgtgcag
     8341 gttttccatc taaagaatgc caatctaccc cagattgact tctggcaaat gccactgttg
     8401 gtttaagaac ataagctgat tttccatgtg gaatagcaat ccccataccg attcccgttg
     8461 tactttcatg ttctctatca tagatgactt ctttaaatct ggttttatct gtgatcgccg
     8521 ctgttttttc taaacgttgg actaaatctt cgattactcc gtcttttgtt gttgctgttt
     8581 cacctaaaac gattgcttca tctgacataa tatccgtgat agaatcaaaa gtttcagttt
     8641 tcgtataaac atcacttgtt tcagtctctg atagatctgc tactgtcaca ccatttgctg
     8701 cagcaaccgc agtttgttgt aatgtcggtt taaggattct taacattagc acggttacag
     8761 caataccagc aatcactgct acaaaaaaca tggctacgtg atccactgct cctaaaaccg
     8821 ctacgatcgg accgccatga gctacatgat ctgtcactcc tgaaagcatc gcgatcacac
     8881 ttcctgtcat tgccccgatt acaatgcttg gaatcacccg tagtggatct gttgaagcaa
     8941 acggaatagc gccttctgta atcccaaaca gtcccattgc taaggacgcc ttaccagctt
     9001 ctcgttctgt tttcgtaaat ttggttttta aaatcaatga tgccagcccc aatgctaatg
     9061 gagggataca gatagctaca gcaattggcc ccataatgga ataattgcct tctgcaatca
     9121 agccagatcc gaacaaaaag gctactttat tgaacggccc ccccatatca aaagcaatca
     9181 tcgctcctaa gatcatagct agaacaattt cacttcctcc ttgcatccct gctaaaaagt
     9241 gagtcaatga actaaataga agagctactg gttctccaat aacatagata aacacaaaac
     9301 caactgccaa tgatcctaaa attgggataa caataattgg catgatcgat tgtaaagctt
     9361 tcggtactcg cactttcttg attcctaaag caatgaatcc tgctaaaaat ccagtgatga
     9421 tcccgcctaa gaatcccgca ttcgcatcac taccatataa tgctccgttt gccgctaaca
     9481 tcccaccaac aaatccagga acaagtcctg gacgatctgc aatactttgc gcaataaatg
     9541 cagatagaat tggaaccatc aaccccatag cgatacttcc gatactattc atcgttgccc
     9601 aaatcgttcc ttctggtatc ttgatccctt caggtgtagc tgttcctcct aatgttaaag
     9661 aaatcgcgat caataaccct cctgtaacta cgaaaggtac catgtacgaa acgccattca
     9721 tcaaactctt atagattttt ttacctaaat tttctgattc acttttattt tcggaagaaa
     9781 taattgcctc cgtactttta tagactggcg cttttcctgc taaaatatct ctcaccaact
     9841 catctgcatg gtggattcct tcttgcacac ctacggttat caaaggttta ccgccgaatc
     9901 gtgatttatc tattttagta tctgctgcga tgattacacc agctgcttct tcgatatctc
     9961 ttgcagtcaa ttcattttca ataccgatgg aaccatgtgt ttcgactttt acctcggcgc
    10021 caactgcttt ccccgctttc tctaagtttt ctgctgccat gtatgtatgg gcaataccaa
    10081 ctggacagga tgtgattgca ataatttttt tcattttcct accgcctttc tacatttata
    10141 gtatataaaa acggtttctt tttattaagc acaaaatgat tccgtaataa ctgaaattct
    10201 tggaataaat agaaatttcg ctaaaaaaat tgatatacga tttattatgt tgaataaaat
    10261 aatattggaa aatcttacat ttctgtactt ctgcttacgt acatctaaat tattatctac
    10321 attaacaatt tccgtaatta attataaaaa gcttattcca tttaatttca ttctggaata
    10381 agcttcttat tttctaatga gttagtga
//
LOCUS       DACETN010000091        10154 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DACETN010000091 DACETN010000000
VERSION     DACETN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.094"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(560..1600)
                     /locus_tag="IXF98_002184"
     CDS             complement(560..1600)
                     /locus_tag="IXF98_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1556481.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IXF98_002185"
     CDS             complement(1949..2275)
                     /locus_tag="IXF98_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556482.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IXF98_002186"
     CDS             complement(2262..3050)
                     /locus_tag="IXF98_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1556483.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="IXF98_002187"
     CDS             complement(3418..3708)
                     /locus_tag="IXF98_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1556484.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="IXF98_002188"
     CDS             complement(3844..4023)
                     /locus_tag="IXF98_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556485.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="IXF98_002189"
     CDS             complement(4020..4370)
                     /locus_tag="IXF98_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556486.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="IXF98_002190"
     CDS             complement(4363..5688)
                     /locus_tag="IXF98_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1556487.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="IXF98_002191"
     CDS             6138..6752
                     /locus_tag="IXF98_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1556488.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7741)
                     /locus_tag="IXF98_002192"
     CDS             complement(6917..7741)
                     /locus_tag="IXF98_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556489.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(8379..8636)
                     /locus_tag="IXF98_002193"
     CDS             complement(8379..8636)
                     /locus_tag="IXF98_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1556490.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8629..8898)
                     /locus_tag="IXF98_002194"
     CDS             complement(8629..8898)
                     /locus_tag="IXF98_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1556491.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9029..9208)
                     /locus_tag="IXF98_002195"
     CDS             complement(9029..9208)
                     /locus_tag="IXF98_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556492.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9589..>10154)
                     /locus_tag="IXF98_002196"
     CDS             complement(9589..>10154)
                     /locus_tag="IXF98_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556493.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3101 a         1854 c         1458 g         3741 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca
     7201 tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt
     7261 tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat
     7321 taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct
     7381 caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac
     7441 aggctgtaat tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa
     7501 ggagtataag agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag
     7561 cttcagaagt tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta
     7621 atgaaatttc tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt
     7681 cgtcagcaag catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca
     7741 tttcagcaat attgctaaaa tcagctaatt tttctttaga ataaaaaaaa acatttatat
     7801 cagaatcaat aaattctatt tttttattac tatatcgtca tcattatctt gaccatttat
     7861 gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat
     7921 tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa
     7981 tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact
     8041 aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca
     8101 tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata
     8161 tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta
     8221 gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca
     8281 atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa
     8341 acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa
     8401 taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt
     8461 ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag
     8521 ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct
     8581 tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga
     8641 ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg
     8701 acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc
     8761 ataatctctt tttgataata caacaactgt atcttctaca tctttacttg ttacaataag
     8821 tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga
     8881 ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt
     8941 atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca
     9001 cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg
     9061 ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc
     9121 cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta
     9181 accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga aataacccta
     9241 tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc
     9301 aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg
     9361 cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat
     9421 tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac
     9481 ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact
     9541 aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt
     9601 tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc
     9661 tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc
     9721 tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata
     9781 atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag
     9841 gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa
     9901 ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat
     9961 cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta
    10021 tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt
    10081 atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa
    10141 atacaattgt tctt
//
LOCUS       DACETN010000092        10082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DACETN010000092 DACETN010000000
VERSION     DACETN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.095"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(137..1147)
                     /locus_tag="IXF98_002197"
     CDS             complement(137..1147)
                     /locus_tag="IXF98_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556494.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(1159..2112)
                     /locus_tag="IXF98_002198"
     CDS             complement(1159..2112)
                     /locus_tag="IXF98_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1556495.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(2102..3172)
                     /locus_tag="IXF98_002199"
     CDS             complement(2102..3172)
                     /locus_tag="IXF98_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556496.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(3178..4221)
                     /locus_tag="IXF98_002200"
     CDS             complement(3178..4221)
                     /locus_tag="IXF98_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556497.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(4222..5163)
                     /locus_tag="IXF98_002201"
     CDS             complement(4222..5163)
                     /locus_tag="IXF98_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556498.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            5247..5594
                     /locus_tag="IXF98_002202"
     CDS             5247..5594
                     /locus_tag="IXF98_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1556499.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5664..7325)
                     /locus_tag="IXF98_002203"
     CDS             complement(5664..7325)
                     /locus_tag="IXF98_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1556500.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(7871..8419)
                     /gene="aac(6')"
                     /locus_tag="IXF98_002204"
     CDS             complement(7871..8419)
                     /gene="aac(6')"
                     /locus_tag="IXF98_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1556501.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            8972..10075
                     /locus_tag="IXF98_002205"
     CDS             8972..10075
                     /locus_tag="IXF98_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1556502.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         2902 a         2051 c         1810 g         3319 t
ORIGIN      
        1 tttttctgct attgttttat tggtttgtca taattagtga gcttttcttc aaaagaaaaa
       61 ttcctagaaa caaaagtcat taagactttt tattaacaag gctaacccta acgctcctat
      121 cagtttcatt cattttttat cccttgataa ccgaatgtct gcccattttt tctactactt
      181 ggctctttct tacggaattg ctttcttctg ctccgtcggt attgatcaaa agaaattttt
      241 tattatacca ttgggactta taacgcaatt ttgttattaa tttatgccag tcgtgaatag
      301 aaaaatcagc atgtgcctct tcttttggaa aagattctac aaataagtag tcatattgtt
      361 tgaccaattt cccagataat tgttcaagat aatcttctcg ctggttcttt tgtttcagat
      421 aaagatccat tacttttctc ttttgtttct gataattgct tgcctgatct aacctgatct
      481 ttctatggtg agcggagcgg actttcacat tcaatttccg ctgtgcatgt tgcagctttt
      541 cttctgtctg gtgaaatttt ggtaaagtta cttctagagg tcgagatgtt tggatcaatg
      601 tcttcggatg ataggcgatc ccgatccatt tagctaaaga ttcttttttt actggcgaaa
      661 catcacaaag tagtgcgaca taaaattctt cattatatcg agcagaaatc gtagcggagc
      721 gaatcgatcc ttcaactggc cgatgctgat ttatcttgat tagttctttt tgttttggta
      781 actttaagta gccattttcc agatagatcg tacgggtttg gttgttggtc gtataagatt
      841 gccacatact ttttttcgtt ttaagctttg ggaagctggc acgtcccttg aaataattgc
      901 gaaaagcccg atccaaatta agctgtgcat ttgctaaagc aagactgtct acttctttta
      961 agaatgggta ttcttttttt aatgtagcgg gtgtgagcga aaaattggct actcctgttt
     1021 gttgccaact tttctgccgc tttttcagca aatggttata ggtaaatcga gcacatccaa
     1081 aagtacgaat caattgctgt ttttgttctt ctgtcggata catacggaat ttaaaagctt
     1141 ttagcatcta tgtccctcct agttagcaac cagaagagat tccttttgtc gttttagttt
     1201 ttttgcgtaa tatgggactt cctgctcagt tgcatagaca aaatgttctg gtgcgatttc
     1261 acgaagttga cgtacttgtc cgtcatctgg ttcaggttga tatttgattc ttctgcgttt
     1321 tctctcatgg tctggatctg gtagaggaat agcagataac aaactttctg tataaggatg
     1381 aacaccgtga ttatagattt catcacttgt tcccatctct aataacaagc cgttatgcat
     1441 tacaccgatt cggtcactaa tatgtttaac catcgacaaa tcatgtgcaa taaatagata
     1501 ggtcaattga tgttcttttt ggagatcttg caacaaattg accacttggg cttggatcga
     1561 cacatctagt gcagaaattg gttcatcaca aatgataaaa cgcggtttta ctgccaatgc
     1621 tcgtgcaata ccaatacgct ggcgttgccc acctgaaaat tcatgaggat aacgagtacc
     1681 gtggcttgga ttcaatccaa cagttctcaa tagatcatcg actttttttg cacgctcttc
     1741 agatgattta gctagtccat taacatcaat tccttcggca atgatatctc tgactttcat
     1801 acgaggattc aacgaagcgt atggatcttg aaagatcatc tgaacatcat gccggaattt
     1861 cgtcaaacct tttttatctt tgatcttcgt tatatcttga ccgtcgaata atatctcacc
     1921 atcagtaggt tcattcaagc gaatgatcgt acggccagta gtggattttc cgcttccaga
     1981 ttctccaact aaaccgaatg tttctccttc ataaatgtga aagctgatgt catttacagc
     2041 atgtacttca ttcttttttc ctacattgaa atactgtttc aatcctttaa cttcaagtag
     2101 tttatttggc atcagcaatt cctccttgac cgtaagtatg tttttgtttc tctttaaaga
     2161 tagcccagcg acgttggatt tcttctggcg gtgtcacttt gggtgcctgc ggagcaagta
     2221 gccaagttgc tgccttgtgt gtttttgata gttcaaaaaa tggtggttct tgttctgcat
     2281 cgattttcat cgcgaattca tttcttggat agaatgcgtc tccttttggc gggttcaata
     2341 aatccggtgg agaaccagga atagcataca atcgatcgtt cgcactatct aatgtcggca
     2401 ttgaaccaag cagtccccaa gtatagggat gttgcgggtt gtagaaaatt tcttccgatg
     2461 ttccaacttc aacgattctt cctccataca tgacagcgac acgatcagcg acattcgcta
     2521 ctacaccaag gtcatgagtg ataaagataa ttgatgtatc gatttttgtc tgaatttctt
     2581 tcagcagctc caaaatctgt gcttgaatag tcacatcaag cgctgttgta ggttcatcag
     2641 caatcaggat ttccggataa cataccagtg caatcgcgat cacgatccgt tgacgctgac
     2701 ctcctgagaa ctgatgcgga taactgttga tacgtttctc agcttcgggt attccaacta
     2761 atttaagcaa ttctaatgca gctttttgtc cctctttttt tgaaacttta ttatgctttc
     2821 taagggactc agcgacttgc ttcccaatgg gcatcgtcgg atctaaagag gtcattggat
     2881 cttggaaaat catcgctatt tctttaccac ggattttttg catctctctt tctgattttc
     2941 caacgatatc gttaccttta aagaggattt gaccagcatc tatattggcg ttgcttgaca
     3001 gcaagcgcat gatgcttctg gtcgtcactg attttccact tccagattcc ccaacgatcg
     3061 ccaatgtttc tcctttattc aaatgaaaat tgacaccgcg aatcgctcgt acctttccag
     3121 catacgtatc aaaagagatt tctaagtctt tcacttctaa aattttcgtc atcgtcttca
     3181 ctctttcatt ttaggatcaa aggcatcacg caatccatca gcaagtaagt taaagccaat
     3241 catgataacc gataaagtta ctgccggaat ccataataga taaggcagat acaggaatgt
     3301 cttgtaccct tcgctcaaca tcgttcctaa tgaagctgac ggaggcgtaa gacctaaacc
     3361 gataaagctt aaaaaggctt cgaagaaaat agcagatgga atactaaaca tcatttgaat
     3421 aataatgaca ctagagatat tcggcaaaat atgtttgaat gcaattttca ttcttgattc
     3481 acctaatgtc aatccagcta aaacatattc ttgatctttt aatttcattg tttgtgcacg
     3541 aacgatacgt gccattggta tccagtttgt gatggccata gcagccacga ttgaaaaaat
     3601 tcctggttca aaaacgacca acatcaaaat catgacaacg aggtttggaa ttcctgataa
     3661 gatttccaaa aaacgttgca tcacgttatc gacacgtccg cctaacaaac cagaaaccaa
     3721 cccgtaagct acaccaatcg ttacatctag taatgcagca ataaatgcga tcagcaacga
     3781 gattctcatt cccataaata agcggcttaa tacatcacgt cccaaaccgt ctgttcctaa
     3841 aaagaaattt acgtttgaag ggacgtttgc ttgagcatat ttatcaacaa gttcaccacc
     3901 aaccattgct tttccattca atccatcgat attgattccc ggaatacgag gaggcaaatt
     3961 gatataagcc acgttttggg cagttggatc atgaggagaa acaaagattg tgatcacaga
     4021 gatcaaaatg atgaccaata gtaaaaccat tgaaataacg gcagctttgt ttttctttaa
     4081 acggcgccat gaatcttgga taaaattcaa agaaggtgca gtaatctgtt cacgttcttc
     4141 ttcggtattt ttttgtaatg gaagaaattc atccgcaggg atatctgcaa ctgtttcata
     4201 acgctttggt atcatttcca tttatcctcg gctcccttct gaaacacgaa ttcttggatc
     4261 aactagaccg tacaatagat ccacgatcaa aataacaaaa atcagcataa atgagtaaag
     4321 gatcgttaca gccatgatcg taggataatc atttgttgtg attgacttaa cgaattgttc
     4381 cccgatacca ggaatagcaa agatattttc taccaccagt gatccggtca tcaatgctac
     4441 agctaacggt cctaataaag tcatcaatgg aatcaaacta tttcgaagac catgtttaaa
     4501 ggcgatttcc caacggctca atccttttgc ttttgctaat tccacataat cactatgcag
     4561 tacttccacc atttccgtac ggataaaccg agcagagtca gcaagcggcg acatggccaa
     4621 cgcaattgtt ggtaagattg tgtacacaaa gccatcccat tttgcgattg gcaaaacttg
     4681 taatttgatt gcaaagatat attgtaataa aacagcaaat acaaagtttg gaatggaacg
     4741 acctaatatc gctacgagtg tagaagatgt atctgcccat gtattttgct tcatggcaga
     4801 gatcgttcct aaaatcgtcc ctaggactgt tccaaagata attgcctgta accctaactg
     4861 caaggatggt ccgattcttc cagctaacaa atgtgcgact ggctggtttt taaattggaa
     4921 ggaaattcca aaatctcctt gaagcaaatt agaaagataa atcccatatt gtacgataac
     4981 tggtttatcc aaaccgactt gtttatttag catctcgatt gtttcaggac tcaaacgttc
     5041 ctgattcgta tatggcgtgc ccggcagcaa ttgcatcaag aaaaaggtga ttgttgcaat
     5101 taaccaaagc gtgatgatca tgaaaaagac acgttttaat acatatttga tataactatt
     5161 cactaatgtt tccccctagc ttttgacttc tgacagatgt ttcgctacat tattagataa
     5221 tgaaccaaaa ataaaggagc aatcaaatga aaaaaaaatc tatcccctac gcagtagctt
     5281 ttctactgat tctggttatt ttaataaaaa atctcatcaa ccattccttt acactcattc
     5341 aattatctaa tgatctattc ttatggtcat tgccgtttct gattatcggc ggttttttat
     5401 gggtcttttc ttcgggtttc ttcgatcatt ttcagcgatc ggtccatctt gcacgaacaa
     5461 ggaaccgaaa gaaaaaacca gaattttcct cgctctcgtc tgccagctac ggtatgtaca
     5521 gcttttggct gatcattgcc ggaatactaa ttgctctttc tgctattttc atgctttttt
     5581 ctttgttagg ataagtgaat atgagaacga ggttgcgaca tttgccgcag cctcgttttt
     5641 tctcatgctc acggtattaa cgattattct tcaacgtatg tccatttgta atcccatgaa
     5701 gcgcctgccg catggtgaac gatacctttg attttttcat tcactaggtg accttctgct
     5761 ttttggtaca ccggaacgac acccatatca tcagagatga tcttgattgc tttttggaaa
     5821 tcatcccaac gggcttgttc atcgccagcg tctgttgatg aagctgcttt aactgcttcg
     5881 tcatattctg cattgctcca gcgaccacgg ttgtatgaat tacctgtgac aaacaaatca
     5941 gtaaagctgc ttgggtctgc atagtcagca gcccatccgc ctagtactgc gtcaaaatca
     6001 cccgaagttg agcggtcaag acgtacagag aatggaacag gtgttggttt gactgttacg
     6061 ccatccaagt tttcttggat tgagttttga acatattcga ttactttttt cgatgaatca
     6121 tcatcagaag ccatcaaatc aaattctaaa gaatcaacgc ccaattcttt tttcgctttt
     6181 tcccaatatt cttttgcttt atctttgtca taagaaacta attttccagc ttcttttgca
     6241 aagtcttcgt tcgttgttgg actttttacc atatctgatg ggatcaatcc tgtagagaca
     6301 actgatccgt cacctagtac ttgttttact aaagcttcac gatcgataga ataagaaata
     6361 gcttttctta ggttcacatt gttgaatggt gaattctttt cacgttgatt gaattcgata
     6421 tagcttgtcc gcgcttcttt aacagaagta taagctggat cgtttgcgtt ttgctgtgct
     6481 aactcaccag acaagatcac gtcatctgct tggccatctt tgaataagtt caatgcagta
     6541 gaagattctt ttactacact gactttgatt tcagaaagtt ttactgtatc tttgtcccag
     6601 taatgatcat ttttcttata agtccattct gtatctgttc ctggtccatc aaaatcttct
     6661 aatgtgaatg gtccattata aacagcttta tcacttgcag atgcataggc atccccattt
     6721 tcttccacta cggattgttt ttgtgggaaa aatgatggga aagcaagcag atattggaaa
     6781 tatggtgttt gtttcgtcaa tttgatttct aattcatagt caccaactgc tttgatccct
     6841 agttctgatt tatctttctt tccagctgta atgtcttctg cattttcaac tggcgcatac
     6901 aggtaagcat attcagaagc tgtagctggg tctactgttc gttgccagcc ataaacgtaa
     6961 tccgctgctg taacaggatc accatttgac cattttgcgt cttctcgtaa tttgattttg
     7021 taagttaatc cgtcttcact gacttctgcc aactcgctag ctcctgccgg ctgtggtttg
     7081 ctgtcttcat caagacggta aattccttca taaacgttat tcaatgctgt aaaactgatt
     7141 gtatctgttg caactgataa gtcagctgta ggcatttctt gaggcacgac taagttaaat
     7201 acgcctcctc catctgctga accgcttttt gctgaactac tgcttgtggc tgaactgccg
     7261 ccataacatc cagcaagtac aaggccgcat accgcaacca aaccaaatgt aaatttcttt
     7321 ttcattttac gtaccccttt attggaattt tcagaataat taatattttc tgaagttgtt
     7381 taagagttta atgaataatt attaaaaaat caacactttt ttttgtcttt ttcataaaat
     7441 aatgattttt tcgtgaggtt tcaaggaatt tttcagaaaa aatagctttg tacaaggttt
     7501 tccatggtct tccaaaagcc attaaataat tttcgttata ttgtctatta ttgccataca
     7561 aagataaaaa agaataaaca aaaagaaact gtgacaaaag tcttgtcaca gtcccttatc
     7621 tgaataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
     7681 agaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc
     7741 tctggtacac ggtgttccaa aagtagcttc ttcggaaata agttggaatt tcacaaaaat
     7801 tcaagaagta attttcgtaa attccttctt atttcttgaa gctgattact tctgtcacaa
     7861 cctcttttta ttattgagaa tttggtcgag gaataatcgt ttttgccatc caaatatccg
     7921 gtttgtccca gccatttgca tttggtaaga cacctacgat tttataacct aatttttcat
     7981 agaattcata cggatgttca cgaaggttct ggatggaagc cactttatca aatgtatgct
     8041 catacaggtc cgtttgactt aacgttgttc catggtctaa atcatccgta cctaaataaa
     8101 tcgtgattcc tcctctggaa gctacttctt tttctaagta attgactaat cgagtaccta
     8161 tttggttctt tcgtcgggag ctttctacaa ctaatggatg caattcccaa cctgtgatac
     8221 cgtattgagg gattgcacca ataaatccta ctaactcatc ttggtctacc gctgctaccg
     8281 cgattcgttc tggattcatc atttcttcta cttcttctgc cgagctgtct ccatattctt
     8341 ccggccaagt cagtctcagt aaatcagaaa gctgatcttt caatactgga ttattacggt
     8401 caaattcact gattatcata cgtcttcccc cttgttcttg cttattctaa cagtcctttt
     8461 gaaaaaacga aatgttctcg ctcaaaaatc tttagctacc aatcagtatc tctattcttt
     8521 cattatttat cttacttata aaataaaaaa attatacaga aacactttat tcaaaatagg
     8581 aaagatcagg aaagccatca attggaggaa aaaactctgt ttgttcatca tgatatagat
     8641 tatcttttgc tttaaaaaaa accaattatg aattgacaaa taaaacctgt tttcatataa
     8701 taatcttaat ttttagaatt tttagataat tacaaaaaag caaagggaat cggtagaaat
     8761 actaagagga acaaaaattc attattgtcg ttttcaatgt ttttgtactt gttcgttttt
     8821 aggatactta ttgatgattt caaggtgttc atgccacctt attcatcact tatcctgtct
     8881 taggttcacc tctactccat ctacagccgc agtcagatcg atcactcttt aaaattttaa
     8941 cccgtttcaa cgaagaaaga aggaaatgaa tatgagtcaa agtgttttct taccaaatta
     9001 taccattgga acagatgcgt atgaaaaagc aaatggaatc gtcaaagaat atgggacaaa
     9061 agtagtgctg attggaggga agactgcact ttcaaaggca gaaaacaaaa taaaagtagc
     9121 tttgacacag gaaatagagg ttgtcgccag tctctggtac gggggtaatt caacttatgg
     9181 aaatattgaa cagttgatcc agcatgaaag tgtccaaaaa gcagacgtcc tgtttgcagt
     9241 cggtggtggc cgtgcttgcg atactgtaaa agtcattggt gaaaaactag ctaaaccagt
     9301 cttcaccttt cccactattg gttcgaactg ttcgcccgtc actgcggtat gtgtcatcta
     9361 taacgaagaa ggcgtaatgg acggactctt ctttccttca aaaccacctg tacacacctt
     9421 cattgataca gcaattattg ccgaagcacc tgataaatac ttatgggcag gtattggcga
     9481 tgctttatcc aaagaagtcg aatctacttt ttctgctcgt ggcgatgatc taggctacac
     9541 ggatagatta ggaatccaaa tcggaaaaat gtgcattgat cgtctcttga cttatggtac
     9601 gcaagcattg aaagatagtg ccgacgatcg tccctcatat gcaattgaac aagtcgtttc
     9661 ggatatcatt gttactaccg gattggtttc tgttttagtt aacaataact ataattctgc
     9721 tatggcacac tcattctatt acggtacgac tgttctagaa aaaatggagg gataccttca
     9781 cggagcatct gtcagttatg gtgtactgac cttattgatg atggatcagc agttcgattt
     9841 gtttgataaa atctatgtgt ttatgaaaaa gaaccaactt ccagtatcgt taaccgacat
     9901 gaatctcaat gtcacgaccg atttagaagc aatcttagat aaagccatgg caacaaatga
     9961 tattaaacat gtgccttatg aaatcacccg agaaaaattt aaacaggcca ttcttgacgt
    10021 agaaacttat catgctacac aagaaagcca gcaaggatat aagaaaatca gttaaataat
    10081 tg
//
LOCUS       DACETN010000093         9935 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DACETN010000093 DACETN010000000
VERSION     DACETN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.096"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            201..680
                     /locus_tag="IXF98_002206"
     CDS             201..680
                     /locus_tag="IXF98_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556503.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IXF98_002207"
     CDS             713..2941
                     /locus_tag="IXF98_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1556504.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="IXF98_002208"
     CDS             3070..4713
                     /locus_tag="IXF98_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1556505.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="IXF98_002209"
     CDS             complement(4786..5331)
                     /locus_tag="IXF98_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1556506.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="IXF98_002210"
     CDS             complement(5354..5839)
                     /locus_tag="IXF98_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1556507.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="IXF98_002211"
     CDS             complement(5829..6641)
                     /locus_tag="IXF98_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1556508.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="IXF98_002212"
     CDS             complement(6780..8312)
                     /locus_tag="IXF98_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1556509.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="IXF98_002213"
     CDS             complement(8421..9452)
                     /locus_tag="IXF98_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1556510.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3299 a         1837 c         1913 g         2886 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aagttgtttg cttattgatg gattg
//
LOCUS       DACETN010000094         9873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DACETN010000094 DACETN010000000
VERSION     DACETN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.097"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..266)
                     /locus_tag="IXF98_002214"
     CDS             complement(21..266)
                     /locus_tag="IXF98_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556511.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IXF98_002215"
     CDS             complement(381..959)
                     /locus_tag="IXF98_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1556512.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IXF98_002216"
     CDS             complement(1036..1566)
                     /locus_tag="IXF98_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1556513.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IXF98_002217"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IXF98_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1556514.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IXF98_002218"
     CDS             complement(2423..2818)
                     /locus_tag="IXF98_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1556515.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IXF98_002219"
     CDS             complement(2815..4227)
                     /locus_tag="IXF98_002219"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1556516.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IXF98_002220"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IXF98_002220"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1556517.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IXF98_002221"
     CDS             complement(5082..6542)
                     /locus_tag="IXF98_002221"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1556518.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IXF98_002222"
     CDS             complement(6712..7869)
                     /locus_tag="IXF98_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1556519.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IXF98_002223"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IXF98_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1556520.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IXF98_002224"
     CDS             complement(9336..9863)
                     /locus_tag="IXF98_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1556521.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
       61 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
      121 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
      181 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
      241 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
      301 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACETN010000095         9820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DACETN010000095 DACETN010000000
VERSION     DACETN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.098"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            55..507
                     /locus_tag="IXF98_002225"
     CDS             55..507
                     /locus_tag="IXF98_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556522.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            521..1210
                     /locus_tag="IXF98_002226"
     CDS             521..1210
                     /locus_tag="IXF98_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556523.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1335..3686
                     /locus_tag="IXF98_002227"
     CDS             1335..3686
                     /locus_tag="IXF98_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1556524.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3766..4161
                     /locus_tag="IXF98_002228"
     CDS             3766..4161
                     /locus_tag="IXF98_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556525.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4486..5103
                     /locus_tag="IXF98_002229"
     CDS             4486..5103
                     /locus_tag="IXF98_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1556526.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5144..5443
                     /locus_tag="IXF98_002230"
     CDS             5144..5443
                     /locus_tag="IXF98_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556527.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5477..5644
                     /locus_tag="IXF98_002231"
     CDS             5477..5644
                     /locus_tag="IXF98_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1556528.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5689..6294)
                     /locus_tag="IXF98_002232"
     CDS             complement(5689..6294)
                     /locus_tag="IXF98_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1556529.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6419..9406
                     /locus_tag="IXF98_002233"
     CDS             6419..9406
                     /locus_tag="IXF98_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ1556530.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9555..9764
                     /locus_tag="IXF98_002234"
                     /pseudo
     CDS             9555..9764
                     /locus_tag="IXF98_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3359 a         1479 c         1879 g         3103 t
ORIGIN      
        1 ttatcttatc gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa
       61 aaaacgatac taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg
      121 aatttagatt ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat
      181 aataaaaaaa tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa
      241 gaatttggtg atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca
      301 gataaaattg ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta
      361 gttagttata aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt
      421 tcgattaagg atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa
      481 gaaactaatt cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt
      541 ttctgttggg ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga
      601 agttatttca gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga
      661 taaagtactt gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat
      721 ggataatcca gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc
      781 agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc
      841 aattatgcct cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact
      901 agcaggagga ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg
      961 tatgggtgta aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga
     1021 cgatggaaga gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg
     1081 aatggcattg tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac
     1141 aatgaatata ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca
     1201 aaatatttaa taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt
     1261 tgacaaacac taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa
     1321 agcaggggat ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat
     1381 acagtaatgt tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa
     1441 tctatggact tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt
     1501 ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc
     1561 tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag
     1621 gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg
     1681 atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt
     1741 tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc
     1801 agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt
     1861 atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc
     1921 ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg
     1981 aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt
     2041 ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat
     2101 tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag
     2161 gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc
     2221 aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat
     2281 atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg
     2341 aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg
     2401 cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg
     2461 cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca
     2521 tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa
     2581 tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa
     2641 tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga
     2701 atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag
     2761 gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat
     2821 ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg
     2881 ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt
     2941 ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca
     3001 tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa
     3061 ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt
     3121 tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata
     3181 gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga
     3241 aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa
     3301 catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa
     3361 aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg
     3421 ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt
     3481 cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa
     3541 tttcattttt cattgataaa atagttaata attcacttgg gacagatttt acttatcttg
     3601 attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc
     3661 aagaaaatag aagtaaactt aattaagaat cgtttactat ttaaacagtg tccttttcct
     3721 ttttaaatat atacgataaa ataagtatat gaattgtaaa aggacatgat gattatggaa
     3781 gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc
     3841 tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat
     3901 tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta
     3961 ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat
     4021 gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac
     4081 aattatattg taaaggcaaa agtatcaaaa agatttattt ttaaccctaa attagaaata
     4141 tatagtgttg taatagaata atagtgttaa aaaaattgta aaagttaaag aagtcaaatt
     4201 ctgatgtgaa aaataaaata taccatggaa gacataaaag aaaaactgga taactagttt
     4261 atttaatggc agaccgttaa ggcctgccat ttttatgtat tttagcggaa taaatataat
     4321 catgcgggag tgtaaactta ctgtttcaaa aagtatactt tttgacatgc cttgattttc
     4381 tttgtttatg tatggcataa taaccacata atacaatttg aaacgtttct gatttaatct
     4441 gattgttttg aaacaatttt agtgaaaaaa gggtagataa aaacgatgag aaaatttttt
     4501 tatatccggg tcagtagtta tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa
     4561 ttaggaatcc ctgaagaata tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc
     4621 cctgaatatc aattaatgaa acggatgttt cgaaaaggag atttactcta tctacaatca
     4681 ttagatagac taggcagaaa taaacaaatg attttagaag aatggcgaga attaactaag
     4741 gaaaagcaga ttgatatttc tgtgttagac atgcctctgt tagatactgc tagttacaag
     4801 caactagatg ggttagagac gcttatctct gacttaattc tgcaattact cagctatatg
     4861 gccgaagacg agcggaaacg aattaatagc cggcaaagag aaggcgtaga agctgcgaaa
     4921 caaaagggtg taaaattcgg cagaaagaaa attgctttac ctcctgaatt tcctgcgatc
     4981 tattctgact ggaaagcagg aaagataact gcagtctatg ctatggatca attgggcatg
     5041 aaacgaaata ccttttatcg acgggtgaaa gaatatgaac ataccttgca ccacttttct
     5101 tgatagatat aacagctatt agacgggaag ggagagtttg aaaatggact taacgaatca
     5161 aagggtcgtt tttactggaa cgctgcagca aatgacacga acccaagcca tcggtttggt
     5221 tcactcttta aacggccatt gtcaatcaag tgttacttca aaaacaaatt tcctggtttg
     5281 tggtcagtat tctaagtcgt tgtttgatga ttctctctat acaaaaaagg aacaaaccgc
     5341 gcgggacttg attgcgttag gtcatgagat taccatttta tcagaagtcg aattttatgc
     5401 gttaattagt caacagttga aagaatggca acggtattta taatcgttag taaaatttac
     5461 aaggaggaaa atttttatga agcgaaaatc ttatgatcgt tggggcattg aaataactag
     5521 ttttaatcga aatattgctt ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt
     5581 ttatcaaaaa aattactttt actgtcctaa atgccatcgc cgatacgggg tacatggaac
     5641 atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct acaaatcatt
     5701 cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg caatctctgc
     5761 aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc gtttttgcgg
     5821 atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg ctttttgagc
     5881 caataatact ccttgccgct gacgttcacg aattttctca cgctcgtttt gtgcaatata
     5941 acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat ttccagtatt
     6001 aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt tcataaaagc
     6061 aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg taacgatgac
     6121 gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct cagtatcttt
     6181 accactggct ttttcttgga ataatttatc aatgttaagt ccttccatca ttgccaattg
     6241 tcgatccaaa ttttgatctt gtgaagatac tcgaatatag ccaatttttc ccataaaaaa
     6301 ctccttttta aaagtgatat aatttacatg taaaatttac ttgtagtttt actgctatca
     6361 tagcattttt aaaaataaat tttatgtaga cttaaaattt acttcatgga gggtttaaat
     6421 ggatgaaatc aaaagtaaca aaaaagcatt tggcatttat ttaggagaac ctagtacaga
     6481 acaattaaac caattttttt atttgcatga aaaagattta gaattgatgg agactatacg
     6541 acgaccagct actagattag gttttgcact acagttagtc tcactacgtt ttttaggaac
     6601 ttttatatta gatcttacca atattcccca aaatgtattg agttatgtag caactcaact
     6661 aaatattgat cctggagaaa ttatctacta tagcaggaga cagaccaggt atcagcacac
     6721 aaaattgata aaatcacatt atggatatca agatttcaac caaccggaaa tagaaacata
     6781 cctagctaac tggcttttaa atagagcgct gtatacaaca gaatcttcag atatgttgtt
     6841 tgatatgctt ctaaaaaaat gtctggatga aaagattgtt ttacctggaa ttactacttt
     6901 ttctcgtttt gttgctagtg caattgaaaa agctgaagag catttatacc gacaattgtc
     6961 tttggttcca actgaagatg agagaaagcg gcttctcaat catttagaat taattggaac
     7021 tccagtttat ggggcaataa tcaaaatgga cattttgaga accccgttag ctgacgatag
     7081 tcataaagaa atagccagag gttttgaccg actaaagcaa tttcaagctt tctctactga
     7141 aaaatggaag atggaatcta ttcctgaagg gaaaataaaa gttcttgcaa attatgcatt
     7201 taaagctaaa gctcaattaa ttcaacgaat gagtgaacag aaaaaactgg cgttgttagt
     7261 ggcatttatt ttcatataca aacgtaaggc tatggatgaa caaattttag cactatctaa
     7321 tttttttgaa actgttttta gacgcgccaa aaataaggaa tctaaagaac gtcttcgtac
     7381 cataaaagat ctagatcgag ccgcatcgac actttcagat attgtagatt tagtaatgtc
     7441 cagtcattct gatactaatg aactatccct tcgcgatcgt atcttagaaa aatattctga
     7501 ggaaaatgtt accgaagctg tatcgcaagt tcggctattg gttaaaaatg atcgtgaacc
     7561 aatagccatt tctgaacttc tgaacactta tcgaaagttc agaaaattta ttccggatat
     7621 tttaactaca ctaacatttg acagcaatga ctacggtaac aactgtaagg aactctggac
     7681 cttcattaat aatagatttc ctaagccgat tacttacaaa atgtttggag ctattaaaca
     7741 tactcttcca aagaaatgga tttattacat tcatgaatac cctgaacaaa ctaataaatg
     7801 tgtattgatt gcagctgtcg aattgttaat tcaatcttta aaacgccatg atatttatat
     7861 tcctcagagt gtcttatata acaatcctat ggattgcttg attgatgaga aaacttgggt
     7921 tcaacaaaaa tacgttttat tagaacaact agatcttcct tcttcagcaa atgaaatgtc
     7981 tttatggtta aagaacgatt tggaactgtc ttataaagaa gcgctaaatc gttggcctac
     8041 ttctaaaatg gccagaatag aaaatgcaga tcgcctggtt gtctctagac ttaaaaagac
     8101 atatgaaaaa aaggaagata agtcttttag acgtcgcatt cagcagttac ttcctaaaat
     8161 tgatttgtca gatttgttat tggaagtaaa ccaacagtta aatcttactc aatcatttaa
     8221 ccacttaagt gacaaggaca ccaaaatgaa agacttggat attagtttat tagcagttct
     8281 tttatcagag tcctgcaata ttggtttttc cccggtttca aaagaaggat ttgattcttt
     8341 aaagtatgat cgtcttgtct atgtcaatca tcattatatt cgagtggata cactttcagg
     8401 agctaatcaa aaaataatac atgcacacag gaaacttgcc acctctaaga tatggggtga
     8461 tgggcatatg gcttcagctg acggtattcg ttatatcaca ccacaacgtt ccctatattc
     8521 tagaagtaat ccaaaatatt ttggccgtgg tcgtgggatc acattctaca attttgtctc
     8581 tgatcaatat atagggttcc acggaatggt cgtttcaggg acactgcgag attcgcttta
     8641 ccttctagaa ggattcctca atcaacccag cgaattaact cctaatcaga tcatgactga
     8701 tacagctggt tatagcgata tgatttttgg tctctttggc ttattagggt ttcaatttag
     8761 tccaagaatt gccaatgata aaggaaccaa actttggcga atcgacgcta acgctcacta
     8821 cggggaactt gatacgttgt ctcaaaattc aattaatatg gaactgattt atacccattg
     8881 ggaggacatt cttagggttg caggttcgtt gaaatctgga aaagtgaatg caacagaatt
     8941 aacgaaatct cttcaacgga atgggcaacc cacgccttta ggaaaagcaa taactgagta
     9001 tggaaaagtt tataagacga aacatcagct tcgttatatt tctgacgaaa tatatgcacg
     9061 gcaaatttta gaacaattga ataaggggga agctcggcat gccttatgcc gtaatatttt
     9121 ttatggacga aaaggaaaac tctaccagac ttattatgat gggatggaag agcagctcaa
     9181 tgcattagga ttagttacta atgccatcat atattggaac tcactgtatt tagaaaaagt
     9241 atttgaacaa atgaaagctg aaggattcga ctgttccgaa gaaatgatac gcaggcaatc
     9301 tccactaatt agtgatcaca tcaattttgt tggtaagtat acgtttagat acgaagctga
     9361 attatccaac ggtaagttac gtccattgaa tgtagaagaa ttctagcaat gttcgggttt
     9421 tgcatgctac cgcactattt cgtatgactg ttccagcgaa tcacttcgaa tagaaaaaga
     9481 gaaaaacacg agaacagcgt taaatcaacg tttcttgtgt ttttactttt gtgctaccgc
     9541 actatttcgt ttctatgttc catgtacccc tttatcaaga agcgcaaaca cgccaaattg
     9601 ccgattggtt gatcggcatg aacgcaagcc ctttatatac cttgaattta caacaaaaag
     9661 gcgtacaagg tacattttca ttaggacgtg tacaaacacc gactttatat ctcatttatc
     9721 aacgccagga agcaatagaa aactttaaaa aagaaccttt tttcctgaat aattcatagt
     9781 tttactaata gtctttccta acattgattt aatagtcttt
//
LOCUS       DACETN010000096         8780 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DACETN010000096 DACETN010000000
VERSION     DACETN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.099"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(310..807)
                     /locus_tag="IXF98_002235"
     CDS             complement(310..807)
                     /locus_tag="IXF98_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1556531.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IXF98_002236"
     CDS             complement(847..1656)
                     /locus_tag="IXF98_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1556532.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IXF98_002237"
     CDS             1990..2847
                     /locus_tag="IXF98_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1556533.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IXF98_002238"
     CDS             2840..3742
                     /locus_tag="IXF98_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1556534.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IXF98_002239"
     CDS             3775..4125
                     /locus_tag="IXF98_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1556535.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IXF98_002240"
     CDS             4159..5097
                     /locus_tag="IXF98_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1556536.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IXF98_002241"
     CDS             5175..6068
                     /locus_tag="IXF98_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1556537.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IXF98_002242"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IXF98_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1556538.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IXF98_002243"
     CDS             6683..6871
                     /locus_tag="IXF98_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1556539.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IXF98_002244"
     CDS             6884..7786
                     /locus_tag="IXF98_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1556540.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IXF98_002245"
     CDS             7818..8060
                     /locus_tag="IXF98_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1556541.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IXF98_002246"
     CDS             complement(8316..8687)
                     /locus_tag="IXF98_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556542.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3056 a         1520 c         1705 g         2499 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaaga tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat
//
LOCUS       DACETN010000097         8736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DACETN010000097 DACETN010000000
VERSION     DACETN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.100"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(479..1066)
                     /locus_tag="IXF98_002247"
     CDS             complement(479..1066)
                     /locus_tag="IXF98_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556543.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1241..2563
                     /locus_tag="IXF98_002248"
     CDS             1241..2563
                     /locus_tag="IXF98_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1556544.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2718..3494
                     /locus_tag="IXF98_002249"
     CDS             2718..3494
                     /locus_tag="IXF98_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1556545.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3487..4056
                     /gene="rnmV"
                     /locus_tag="IXF98_002250"
     CDS             3487..4056
                     /gene="rnmV"
                     /locus_tag="IXF98_002250"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1556546.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4049..4933
                     /gene="rsmA"
                     /locus_tag="IXF98_002251"
     CDS             4049..4933
                     /gene="rsmA"
                     /locus_tag="IXF98_002251"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1556547.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5384..6013
                     /locus_tag="IXF98_002252"
     CDS             5384..6013
                     /locus_tag="IXF98_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556548.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6137..7246)
                     /gene="ugpC"
                     /locus_tag="IXF98_002253"
     CDS             complement(6137..7246)
                     /gene="ugpC"
                     /locus_tag="IXF98_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1556549.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7455..7877
                     /gene="mgsA"
                     /locus_tag="IXF98_002254"
     CDS             7455..7877
                     /gene="mgsA"
                     /locus_tag="IXF98_002254"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1556550.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8200..8736
                     /locus_tag="IXF98_002255"
     CDS             8200..8736
                     /locus_tag="IXF98_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1556551.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2765 a         1543 c         1790 g         2638 t
ORIGIN      
        1 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
       61 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
      121 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
      181 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
      241 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
      301 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
      361 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
      421 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
      481 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
      541 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
      601 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
      661 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
      721 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
      781 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
      841 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
      901 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
      961 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
     1021 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
     1081 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
     1141 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
     1201 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
     1261 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
     1321 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
     1381 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
     1441 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
     1501 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
     1561 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
     1621 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
     1681 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
     1741 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
     1801 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
     1861 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
     1921 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
     1981 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
     2041 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
     2101 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
     2161 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
     2221 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
     2281 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
     2341 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
     2401 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
     2461 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
     2521 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
     2581 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
     2641 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
     2701 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
     2761 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
     2821 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
     2881 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
     2941 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
     3001 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
     3061 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
     3121 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
     3181 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
     3241 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
     3301 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
     3361 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
     3421 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
     3481 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
     3541 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
     3601 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
     3661 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
     3721 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
     3781 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
     3841 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
     3901 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
     3961 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
     4021 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
     4081 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
     4141 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
     4201 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
     4261 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
     4321 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
     4381 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
     4441 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
     4501 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
     4561 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
     4621 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
     4681 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
     4741 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
     4801 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
     4861 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
     4921 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
     4981 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac
     5041 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
     5101 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
     5161 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
     5221 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
     5281 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
     5341 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
     5401 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
     5461 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
     5521 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
     5581 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
     5641 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
     5701 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
     5761 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
     5821 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
     5881 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
     5941 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
     6001 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
     6061 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
     6121 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
     6181 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
     6241 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
     6301 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
     6361 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
     6421 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
     6481 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
     6541 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
     6601 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
     6661 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
     6721 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
     6781 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
     6841 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
     6901 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
     6961 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
     7021 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
     7081 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
     7141 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
     7201 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
     7261 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
     7321 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
     7381 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
     7441 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
     7501 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
     7561 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
     7621 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
     7681 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
     7741 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
     7801 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
     7861 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
     7921 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
     7981 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
     8041 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
     8101 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
     8161 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
     8221 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
     8281 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
     8341 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
     8401 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
     8461 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
     8521 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
     8581 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
     8641 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
     8701 aaaattttaa gaagtttagc gatccacgct aaataa
//
LOCUS       DACETN010000098         8287 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DACETN010000098 DACETN010000000
VERSION     DACETN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.101"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            216..1409
                     /locus_tag="IXF98_002256"
     CDS             216..1409
                     /locus_tag="IXF98_002256"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1556552.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1539..3011
                     /locus_tag="IXF98_002257"
     CDS             1539..3011
                     /locus_tag="IXF98_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1556553.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3094..3882
                     /locus_tag="IXF98_002258"
     CDS             3094..3882
                     /locus_tag="IXF98_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1556554.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3949..4617)
                     /locus_tag="IXF98_002259"
     CDS             complement(3949..4617)
                     /locus_tag="IXF98_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1556555.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4732..6444
                     /locus_tag="IXF98_002260"
     CDS             4732..6444
                     /locus_tag="IXF98_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556556.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6447..8219
                     /locus_tag="IXF98_002261"
     CDS             6447..8219
                     /locus_tag="IXF98_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556557.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2538 a         1387 c         1821 g         2541 t
ORIGIN      
        1 ttaaaaagct tagcagagtt atctttatgg atgattgtgc taagcttttt tggacagttc
       61 taatgtaaaa tatttttata tttttaagca aaaaaatgaa aaaatacgag cagaaaactg
      121 ttttatctgc tatacttggt tcgttgcaag ttcccgaaag gatccgcagg acggagatgc
      181 cttgtaaccg aatttaacta gggggaatca ttataatggt agaaagacac ttatttacat
      241 cagaatctgt ttctgaagga catccagata aaattgctga ccaaatcagt gatgcaatct
      301 tggatgcaat tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa
      361 ctggtcttgt tttagtattt ggagaaatat ctacgacggc atatgtagat attcaaaaag
      421 tcgtacggga aacaataaaa gaaattggtt atacacgtgc aaaattcgga tttgatggag
      481 atacagcagc tgtattggtt gcgattgatg aacaatctcc tgatattgct caaggagttg
      541 atgaagcact tgagatccgg gatgaagata aaaaagacgt attagatgag attggtgctg
      601 gtgaccaagg tttaatgttt gggtttgctg tagatgaaac accagaattg atgcctttac
      661 caatcgcttt gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat
      721 taacgtattt acgtccagat gcaaaatctc aagtaacggt tgaatatgat gatcaaggac
      781 aaccggaacg cgtagataca atcgttattt caacacagca tgatgatgca gtagataatg
      841 aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga agttattcca gctgaattat
      901 tagatgatca aacgaaatat tatatcaatc cgactggccg atttgtcatt ggtggtcctc
      961 aaggggatgc cggattaaca ggaagaaaaa tcattgttga tacgtatggc ggttatgctc
     1021 gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa agttgaccgt tctgccagct
     1081 atgctgcacg ttatattgct aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag
     1141 tacagctagc ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc aatactttcg
     1201 gtactggaac ggttccggaa gaagaactga ttgctgcagt gagagaaaac ttcgatcttc
     1261 ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg
     1321 cttacggtca ttttggtcgt acagatgtag atttgccttg ggaacatacg gacaaagtag
     1381 atgcgttgaa agcaagttta gcaaaataag ttatattcag ctggaacgat cgccaagtgg
     1441 tggttgttcc agcttttttt atggcagtta acctcttgaa aaagatagta attatttatc
     1501 tatttttaga tcctgtcata atgaaaggga ggaataagat gaaaaaagaa acaaatgtta
     1561 ctttggtcac agtgagtatt ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg
     1621 taacaaccgc tatgccaacg attgtcggtt cattacatgg aatggaaatc atgaactggg
     1681 tattttcgat ttatttgctg acaaacgcta tgcttactcc gatttacgga aaacttgcgg
     1741 ataaagtggg acggaaacca atctttattt ttgggacatt attgtttatt atcggttcgt
     1801 tattttgcgg tttatctcaa agcatgattg ggttgattgc tgctcgtgcg attcaaggga
     1861 tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat cggcgatctt tattctattg
     1921 aaaagcgagc aaaagtttta ggtttgaata gttcagcctg ggggattgcc agtgtttttg
     1981 gaccgcttgc gggaggattc atcgttgaaa cgatcagctg gcactggatc ttttttatca
     2041 atgtgccgat cggaattcta ttaattattt tgatttggct atatttaaat gaaccaaaaa
     2101 tcatacatga gtctaaacca atggatattt ttggtagttt tagtttgatg gccatgcttg
     2161 tctcattgct gttagggttc caattattag gagacgaagg aatgagtccg cttgtttctg
     2221 ctttgctgct aggtagtatc gtaatgttag gtatttttgt ttttgccgaa aaaagagcta
     2281 aagatcctgt catcatgatg catttattca aaaatccact atttgttgtg gtcaacttgg
     2341 ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt ttatatccct atgtggatgc
     2401 aaggtgtatt agggctgaat gcagcaattg gtgggctggt attagcacca ttatcactag
     2461 tctggatggt cggttctttc cttgcaagca agtggatgac tcaaatgaaa actcattggg
     2521 tcttgagaat tggactacta atcgcagcta ttgggggcgc ttggttgtat cttatgcctt
     2581 tcagaagtaa ctatttatgg ttttttgcta tatcaacggt catcggtata ggcttaggtg
     2641 tgaccatgac tacctcgact gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg
     2701 tagcaacttc ttttaatacg ttagttcgaa caattggtca aacagtcatg gtttctattt
     2761 ttggtttatt gataaatggg gtaactaaac gagaacttgc agcagcaaat ttaacaaatg
     2821 atgcagatat catgaataaa ctggtgaatc cacaaacggc taaatatatt gatgcggata
     2881 ttttgttacc attaagaaaa attctttatg atgggttaca ttccgtttat ctagttggct
     2941 tgttactcgt gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa
     3001 taaaacagta gtttcggtta agatatgtac gtagccgaat cattcaaatt taaagttatt
     3061 caaaaataac ttcttactaa ggaaggcgaa aatatggcat ttattcaagc aaatatttat
     3121 tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac taccgcaaac aacaaaaaag
     3181 gtgattggca ccagttcaga gcaagagaca acggatatac ctgttttata tttacttcat
     3241 ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt cgattgaacg ttatgtagca
     3301 aactacggat tagctgtcat catgccatct actgatcttg gctggtacac agatacagtt
     3361 tatgacatga agtattggac attcattgca gaagagctac ctgagatttg tcatgaatta
     3421 ttcccgcaat taacaagaaa aagagaaaaa acatatgccg ctggattatc gatgggcgga
     3481 tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt
     3541 tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt tgatggttcg taaagcatct
     3601 ttttgggaag ggatctttgg accattggat caaatcgaag gttcaaaaaa tgatccgcaa
     3661 tacttattga aagaactagt aaaaagcgga aaaccagttc caagatttta tatggcttgt
     3721 ggggaatctg attttcttta tcaggcaaat caggaaatgg tatctgctat gcaggaacaa
     3781 ggcttggcgg tcacctttga acaaggacct ggcgatcata actggatctt ctgggaccaa
     3841 tggattcaac gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag
     3901 atagctccta tagagatcac aaacatagtg atctctatag gagctttttt actgctcgtt
     3961 ttctcttctt ttattttgtt ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa
     4021 gagtgctttt tcattcaatt ctgcatggga ctgcatactt tctttaaatc cctctaattg
     4081 attttcaact acataattac gcataaatag aactgtttct ttcaaaaatt catttccgtc
     4141 cataattttt tgtcgaagct gctgttcatg ctgctgatcc attaactgtc caatcaataa
     4201 attgtggaga gaggcaatgg cctgatgact ttgggtgact gaaaatcctt cacgaacaag
     4261 taattggatc atttgctcca aatgttctaa tcgactcgga aaagcgggca cagtattgat
     4321 ttctaattct gccccacaag ggaaacgtgt ataaaggtca tagtaatttt ccatatactg
     4381 cagcaactgt tcattccaag gcaaagttgg atctggaagc gctaattctt cttccattgt
     4441 ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa taccaatata aagcaggtgc
     4501 ttgaacatga attctgtttg cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc
     4561 agccaggata tcaaaggcgg cctgaatgat gattgcttga gaaagcttta tttccatttt
     4621 atcaacctcg tgtattgcat tatcttttta aacatggtaa attgttagtc ggtctaattt
     4681 tacagtgtta aattagtcct gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta
     4741 atcttacgac atgcgaaaaa atataaagta gctgttttta tttccttgct gtccgtagca
     4801 gtcatggttg cagcagcatt atggcaacct aagttactac agcaagtatt ggaagctatt
     4861 attacagaag ataatgatga aatgaaaaca attggtattt acctgatcag tctggctttg
     4921 ctaggattag ccgcaggtgt aacgaacacg attttctctg caaaagtagc tcaaggcgta
     4981 agtgcagata tccgagaaga agcatttcga aagatccaga cattttcatt tggaaatatc
     5041 gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa
     5101 aatttagtga tgatttcttt gcaatcactt tttcgaattc cattcttatt tattggtgcc
     5161 ttcattttag ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt
     5221 acggtatttc tgattacggc actttcattt acaaggatgg gcaagcactt catgatcatt
     5281 caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg gattcgtgta
     5341 gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac
     5401 ttaacaaaac acaacatcat agtcggtaca ttattttctg tgatgatccc gtccttcatg
     5461 ctggctgcca atttggctgt ggttggggcg atcttttttg tgagcgatct tgttaaagat
     5521 gatcctacgt taattggtgg aattgcttca ttcatgaact acttgatgca gattatgatg
     5581 gcaatcatta ttggcggaat gatgatgatg atgacttcaa gagcagccgt atcgctgaag
     5641 cggattgcgg aaattttaga tgctgaacca gatcttactt atttagacgt tccagaacaa
     5701 gagttgacgg gttctgtcac ttttgaccat gtttcttttc gttatcctgg tgaggataca
     5761 gatacgctta aagatatcag cttttctatc aaacctggag aaatggttgg tatcgtcggg
     5821 gcgactggtg ccgggaagtc aacactagca caattgatcc ctcgtctatt tgatccgaca
     5881 gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga
     5941 caaacggttt cctttgtttt gcaaaaagca attttattct caggaacgat tgctcaaaac
     6001 ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc ttctattgct
     6061 caagcaaaag aattcatcga aaagttatct gatcgttacg aagcaccaat agaagaacgt
     6121 agcagtaact tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt
     6181 aatccaaaga tcttgatcct agatgacagt acgagtgcac tggatgctcg ctctgaaaag
     6241 ctggtccgag aagcactcga tcgagatttg aagggaacaa cgacgattgt catcgcacaa
     6301 aaaattgctt ctgttgtcaa agcagaccgc atcttagtat tggatgaagg cagattagtc
     6361 ggcgaaggaa cccatgagga attggtagct ggaaaccgtg tctatcagga aatctttgaa
     6421 actcaaaaag gaacggagga gtaagtatga ctgatctaat aaaagcaagt aaatttttct
     6481 atcactattt gaagcggtac aaactctcct ttttctttat ctttatcaca gtgatcattg
     6541 ctacctatct acaagtaaag gcacctcaat acatcgggga agcttttcaa gaattagcta
     6601 attacgtagg aggcttgatg caaggcgtag acgataaaag caaatttgtt tctgtaatat
     6661 ggaaactctt gctgttttat gtactagcta gtgcagccaa ctttatctat agtattttgt
     6721 tcacacaagt agtagggaag tcaacgaacc gaatgcgtat cggtctattc aacaaattgg
     6781 aaaaattgac gatccgtttc tttgattctc atcaagacgg tgaaatttta agtcgtttta
     6841 ccagtgactt agataatatt caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga
     6901 atgctgtttt acttgtcggg atcttgatca tgatgttccg ccaaaatgta gagttagctt
     6961 gggctacaat tgcttccact ccagtagccg ttttgatagc tgtcttgatt atccgcaaag
     7021 cacggaaata tgtggatctt cagcaagatg aagtggggaa actaaatggt tatatggatg
     7081 aaaaaatcag cggacaacgt gtcatcatca caaatggctt acaagaagaa acgattgaag
     7141 gattcttaga gcataatcaa agtgttcgcg aagcaacctt caaaggacaa gcttattctg
     7201 gtctactatt tccgatgatg caaggaatgt cactggtcaa tacagcgata gttatctttt
     7261 tcggcggctg gttagcatta aatggtgatt tggaacgttc ggtggcatta ggcttagttg
     7321 tgacattcgt ccagtattca caacaatatt accaaccact gatgcagatt tcatctggat
     7381 acagcatgat ccaattagcg attacagggg caagacgatt gaatgaaata tttgatgaaa
     7441 aagatgaagt caaaccagaa aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt
     7501 tgcagcacgt agattttggt tattctccag aaaaactagt cttgaaagat gtctcgatca
     7561 atgtggacaa aggagaaatg gttgctcttg ttggtccaac aggatcgggg aaaacgacga
     7621 tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa
     7681 tagatatccg agattatgat cttgatgcat tacgtagaca tgtaggaatc gttttgcaag
     7741 aatctgtatt gttctccgga acgatcaaag acaatatcac attcggcaaa ccggacgctt
     7801 cagatgaaga agtcatcaat gcagcaaaac aagcgaatat tcatgacttc atcatgacgt
     7861 tagaaaaagg ttatgataca gagatatccg aagaaaataa cttattcagt acaggtcaaa
     7921 agcagttgat cagtattgct cgaacgatca tcacaaatcc atctttattg attttggatg
     7981 aagcaacaag taatgtagac acggtaacag aagcaaggat ccaaaaagca atggatgaag
     8041 caatcaaagg aagaaccagc tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag
     8101 atagaatcgt tgtcttgcgt gatggggaag tcattgaaga aggcagtcat catgagttat
     8161 tagcagaaaa tggattttat gcggaacttt atcataatca atttgtattt gaaagttaaa
     8221 ttaaaagaac ctcgttataa cggggttctt tttatatatt ttatgttgtt tgtttttata
     8281 aggggca
//
LOCUS       DACETN010000099         8130 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DACETN010000099 DACETN010000000
VERSION     DACETN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.103"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..475
                     /locus_tag="IXF98_002262"
     CDS             <1..475
                     /locus_tag="IXF98_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1556558.1"
                     /translation="LIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQT
                     VQYNFELAKKFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKP
                     FDILFADEPTGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1168..1548
                     /locus_tag="IXF98_002263"
     CDS             1168..1548
                     /locus_tag="IXF98_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1556559.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1530..2444
                     /locus_tag="IXF98_002264"
     CDS             1530..2444
                     /locus_tag="IXF98_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1556560.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            2684..3373
                     /locus_tag="IXF98_002265"
     CDS             2684..3373
                     /locus_tag="IXF98_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556561.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3494..3580)
                     /locus_tag="IXF98_002266"
     CDS             complement(3494..3580)
                     /locus_tag="IXF98_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1556562.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            4226..4963
                     /locus_tag="IXF98_002267"
     CDS             4226..4963
                     /locus_tag="IXF98_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1556563.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            4991..5530
                     /locus_tag="IXF98_002268"
     CDS             4991..5530
                     /locus_tag="IXF98_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1556564.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(6028..6489)
                     /locus_tag="IXF98_002269"
     CDS             complement(6028..6489)
                     /locus_tag="IXF98_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556565.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            6622..6996
                     /locus_tag="IXF98_002270"
     CDS             6622..6996
                     /locus_tag="IXF98_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1556566.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            7050..>8130
                     /locus_tag="IXF98_002271"
     CDS             7050..>8130
                     /locus_tag="IXF98_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556567.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEEN"
BASE COUNT         3108 a         1275 c         1327 g         2420 t
ORIGIN      
        1 cctaatagat aaacaatata ccggtaactt attctatttt gaaaagaaag tttctaaaca
       61 tgatatacaa cgtctacgaa gaaatcatat tagctattta tttcaaaact atgctcttct
      121 tgaaaatcaa acagtacaat acaatttcga attagcaaaa aagtttaaca gtaatatttc
      181 caatgatcat atttataatt tattaacaga tttttctttg ccaaaagata tcttaaaaga
      241 aaaaatcttt ctcttaagcg gtggagaaca acaaagaata gcattaatta gaaatatgtt
      301 aaaaccattt gatatattat ttgcagatga acctacagga aatttagata aagaaaactc
      361 aacttttata atgaactttc tgaaaaattt aacagaaaaa gaaaaaaaag ccgttgtact
      421 agtaacacat gacttaaatg ttctaaacta tggaactaat attatagaat tataattttt
      481 acccgtgatg ctagctgatt taaaactagc accacgggta tttattttat attccatctc
      541 tcctatccca aaatttaaat aatcacttaa taaaaaggag gcaccctagt gattttattg
      601 aaaaaagagt atgaaaaatt ttatgataga cttatttatt ctttgcaatt gccaaaaaaa
      661 ttaaaccgtc aattacaaaa gacttatgaa actaaatcaa ctgaagaatg ggagagttat
      721 tgctagattt ttggtagaag taacaaaaag tggacatatt atcataatga ctcgccgaaa
      781 gtctgataaa aaattggcta aaaatattgc tgaatgttta ataaaactat cagttttagc
      841 taaatagtta gtagtttaca caaaaaagca tttatgtttt ttgttgcaaa gtaacaattt
      901 catcttcggt gaaataagcg aaactttaca aaaagtggct agcatttgct agcttttttt
      961 gtattgtgga gcgtggcttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1021 cggttatttg cactaaatac gtaggtattt ggctagcaaa tatagagctt gccaaaccct
     1081 tttagtgagc tttactcatt gtaccttttt aggtacaatg gtattgcgaa cgatacgtcc
     1141 gtaggacgta ctgaaagggt gggacttatg agcgaacaaa atcaaaattt ggctagcaat
     1201 tcttctaaaa aatataccta tcgatccgag ccaaaacaaa tcagttttcg agtgagcgaa
     1261 tccgaatttg caaagctgaa gcaatcggct gaagctttgc aaatgagtgt gccggctttc
     1321 gtgaaagcca aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc agacattgcc
     1381 caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgctaagtgg
     1441 tgcaatacac accaacatga cgttgcttct ggtgacgctc aacgtttatc cgaaaaccta
     1501 aaaatcatgc aaaaggagct acaaaagata tggcaacaat taaggtaagc cgaaccactt
     1561 cttgtagtcg tgcaattaat tacgcagaac cacgagcaac cattaaaaca ggaataaact
     1621 gtgacgtcga ttatgccaaa agtgaaatga aacaaattcg catgctgtat ggaaaagacg
     1681 accgtgtaca ggcacactta ttgattcaat cttttcgacc aggagaaatt accgctgaaa
     1741 aagctaatca actaggggca gagtatgcaa aaaaaatagc ccccgatcac caaacagcta
     1801 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
     1861 tggagactgg aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa tgttatgacc
     1921 taaatgacga gatttgtctt gcacataact taccaatcac tgagcgagga aagaaaccag
     1981 aaaaacgaac catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt tggaaagacg
     2041 aaattcggat tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc tatgaccaat
     2101 tttgtaattc cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa aatttcactt
     2161 acgaattgat ccacaaaaag aaaaaagtac gatccaacag actaggaaag gactacgaaa
     2221 aggagacgat tttacatgag ctggacagac gagaacgaac ctataaccaa cagcgaacca
     2281 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
     2341 aacgaactct tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac catgaacaac
     2401 acgttgaaag aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacata
     2461 gaaaacgaaa taaagaaaca acgtgctttt ctcgttcctt atacgcaaga aaggatacaa
     2521 actacgattg atttacaagt taaaaaactc catcaagagc tattagacca gcgaaataag
     2581 gaacagaaaa aggtgcttat tcaacgatca ttggattttc ttgccccctt atgttgctta
     2641 gtcctgacac tgttcgcatt ttggttagta tggggaggct aaaatggcat tttatttacc
     2701 ctatctactc atttttgtta gtatttcagg aagtatttgg ctcatctata aaatttttca
     2761 aacaagatat tcattaaaag gctctaaaat tcgatttaag cgatttttct ttcttggctg
     2821 tatattttcc ttgataatcg tttcaagcgg cttattcggc gttttagagg gaaataagcg
     2881 cgtttctaga agtattttat taggaaatgt cactcaaaag tatgaatcag cgcgtgataa
     2941 aaagaaaaaa gagcaagcat tagcacaaaa aatggaagag tttaccactt gttatgagga
     3001 aatgaacgat atttttgtga atcaagaaaa acgtttgacg gataaaaaca tggaaaaact
     3061 cactcgtctt tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag acaactatga
     3121 gcgaaccaaa aaagacgtgc aatatgtaaa agatacaaag atagaagaag cctgtagtga
     3181 tctatttgga gacacaaacc cttggtttgc cagtgaagaa gaaaaaaaag aaaaacaaca
     3241 atctgtcaca tatgaacgct atgaaatctt acttcaacaa gcaactaaca tgcaaaatcc
     3301 gacaaaaaaa gaaaccgcct taaactatct aaaaagtgtc aaggaatggt tagatcagca
     3361 acaacaaaat tgaatttgat ttaaaatcat gttaaactga atgtgcaaat gatcaaacat
     3421 tagatacacg aaagccccag agaggtcgga ttctctgggg ctttttttca ggttgatgtc
     3481 gcctttattg attttacttc ttgtttcggt tattcaacca ataactgaat aaagagacac
     3541 aaactcctac aagaaggggg caaatgaaca gttcaaacat tagatacacc tccttccagt
     3601 cgcaagactg tcggataggc gacaagttat tatatcttaa aaaattaaat ttgtgtggtg
     3661 tttttaagta tcacttgttt tagggttgtt agggcttgcc ctgaccgcct gtaaggcgct
     3721 tctcgactag attaagggaa atttaatcaa gaattgtctt agaacggctt agacaagctt
     3781 tcagagtctc acacctcctc tttgaacaac ggttttagca aaggagccgg aggcgactgc
     3841 gcagtataat atgagagcat agcgatcaca cctttttagc gaccgtaggg agcaagtttt
     3901 ccagtttaga aaaattcgcg aacctttttg gaggaaaaag taggagtttt ttaggcggga
     3961 aattcaatgt gctcttaaga tatactgtta gatatactgt tagatatact gttaataata
     4021 actgttaggg attagcaacc ttatgggaga gtaagtattg acataggtag tgggggataa
     4081 attgtcacac tatgggggat aaattgtcac actatggggg ataaattgtc acactatggg
     4141 ggataaattg tcacactatg ggggataaat tgtgttttac atcctcttat gtctatgata
     4201 acataattta ttgaggtgat ttttattgag taacgatata gttcgatata ataatggatt
     4261 aaattctgtc cctttaagaa aatttacccc tatagatatg gatttatttt ggggaatatg
     4321 ttctaaaatg aaaagaaaag gtacaaaaga agaaattttt acatttaaac aaataaaaga
     4381 attaattgat tacaatataa atgcacgtgg cgaccgtttt gctaatgatt taataaaaat
     4441 gtcaaaaaaa atcattactt tgtcttttat ttatgaagac gaaaaaacat atgaacaatt
     4501 aaatctgttt caaaaattta aaatcgataa agtgaatgaa acattattaa taaaggtttc
     4561 tgaagaattt gaatatatac ttaattcaat cggaactaat tttactcgtt tcgaattaga
     4621 aaatatgaca aacttaaaga gtagctacgt taaagagtta tatcgccaac ttatggctca
     4681 taaagacaga agatctaaaa aaggtcactg gtttgtaaaa atagaagatt ttagagctct
     4741 attagcaata ccagaaagtt atcgcatgag agatattgat aaacaaattt ttgcccaagc
     4801 gaaaaaagaa tttttaaacg ttaattcaac tcaccctgct atttttaaaa cttttgaaat
     4861 caaaaaaatt aaagcctaca aaggaaataa gataggatct ctgcaaattt attttgaaga
     4921 aaatatacct tcaattacct tagataattg gttagagaaa taatacctaa caatttagga
     4981 ggcaattatc atgcaaccca aagaaacctt tacaataaag gaattatctg aattatttga
     5041 aataagtagg caagcaatct ctaaacatgt tgataaatta gacccgtcct tattagctaa
     5101 aaatgagcgt ggttataagg ttgtattacg ttctggagta ttgcaacttg ctagcaactt
     5161 aggtaaccaa aggttactat ataggttgca tgagtatgaa caagaaaacg ataaaaacgt
     5221 ttatatatca agcaaaaacg tgattgtaaa cgagttgcta gataagttgc gtgaagaaaa
     5281 tgaattttta cgagaacaac tttccctcaa agagcaaaat aattctaata tgcaaaaatt
     5341 actcgaccag caacaacagc tcaccctaca ggcaaatcaa cagatcgaaa aactgcaaga
     5401 acagttacaa ttagcttata cggaagaaac tccagaagat gaatcaacta ctattctttc
     5461 tgaagaaaca gaatcgatag aaaaacacac aaaaaagaag aagcaaaaat ggtggcaact
     5521 ttggaaataa ctggagggaa cattcatgac aagaaaaaat cttctattcg gttctatttt
     5581 actaggaatt ctaggcttca gtccgatgtt attgtcagta atcaataaag ggaataaaca
     5641 tggtgaattg aaattgaatc caaattatta caacagtcct aacgaagaat tgtttgtaaa
     5701 agagacggat aaataataaa aaagactgct cttccgagca gccagttaaa aaataatgag
     5761 tgtttattct tgaaaaagca cgcgcttatg aagcgctacc aaaaatttac catattttac
     5821 aaaaaataac aagtcttaaa aaaatgacag ataaaaatgc acaaagaatt agatatagat
     5881 aaccgcgaaa aatcaagctt tagaaagttg cttttagcta gaccaattaa aaatcacaaa
     5941 gtgagcgtct ctatccattg gtagaggggc tacttggcat agtgtacatt atgtcaacta
     6001 tgcaattaaa taactaacta cttcatatta gcttaataaa ataatcaaaa taactagggg
     6061 gaaaaacatt gtataaaaaa atttttcatt ttttaaataa gaatagtaaa tataaaaata
     6121 atttaagata tctattacta ttaatccaga tagagcaaac caagtaaata ttccatgtgt
     6181 aaagctaaaa aaattcattt gaatcaattc aaataaacct aatatagtaa caaatatatt
     6241 aaaaataata gctgtcaata ccagtaaatt atttttttta ttttttgaat tatcttcatt
     6301 taaaatagaa tttatgttag tactataaaa ttcacaaatt ttttttagtg tctctatatc
     6361 tggtgttgtt ttgttatttt cccactttga aatagtttga cgtgtaacgt taaattttct
     6421 tgccacatat tcttgagtat accctttttt cactcttaat tctcttagct tagatcctaa
     6481 atcctccatt ttttctccta tgtaaacgat tatttacata aaattttatc ataaaaatac
     6541 taacgcgcca aaaaagttct ttattttata aaacacacta aaataaaata attctagtaa
     6601 aaaagatagg agaaataagt tatgaaaaaa tttttatgtc tttctgcttt aatgggtgtc
     6661 ttattaacta gtggaggaat agctagtgcc acagaagctt taaatctaga tgtacccgaa
     6721 gaccataccg ccatttacgg aggaggaatg gaagctacag aagaaggatt ttcttctagt
     6781 aaattgagat atgctgctgg cggcggggat ttcaactgtg gggttaatgg atttaaagtt
     6841 tatgcaaatt actaccatgc tagagctaaa catagtgcaa cagctaaaaa tggacgaggc
     6901 ggtcaagtac gatcagttca gaaagctgga gtaagagctt atgcaacatg caatgccaca
     6961 cttactggaa atactggttg gtggaatgtt tattaaaaaa gagagtttaa actctctttt
     7021 ttatttacat ttgaaaggaa attcaaaata tgttaaaaaa aataaacgct gttttatcac
     7081 tttttgtctg cattacaata ctattttttt ctactaatgt tataaatggt ataaagactt
     7141 ctgaagcgct atataaaaat accgctgaaa ttcatatgca atataaagat gatatggact
     7201 ttaacagtta tataaacgct ttgtatgaaa tatctacaca agaaaatatt aatatttctc
     7261 aatatagttt tactgctgtt aaccaactaa ctattcttgc tacaaaccct aacgctaaca
     7321 aacactggaa attttctaaa aaaaatatat tagcaagaaa cgattctaaa gtccattata
     7381 ctaataacaa aaattctaag taccaattaa aacttccaaa taatttttta aatataaata
     7441 tttatccttt ttctcatgtg gaaaatattg gattaagtga aatattatat gtacaaggaa
     7501 actcaaacaa attaattcct gtactgaaaa aatatggaac acctactatt tccaatatca
     7561 atcaatcaga tacttttcaa attaatacta atattgttct tgttatctgc tatcttagta
     7621 tatttattgt aattactaca attatatttg ctttttcaaa aatgaaagaa atcactctaa
     7681 aaaaaatgtt aggctataac tcttttgata ctataaaaga ttctattaaa gagttaaacc
     7741 tcttattttg ttctttgatt ataactttca ttttatctat ttttttgagc atatattaca
     7801 gtaacgaaaa ttttttccta tcatttcttc tatgtagtat ttttcatttt ataattagtt
     7861 ttttatttat catagttact ctaagtatct atcatttatc tagtataaaa aattatctaa
     7921 aaaattcacg accacttaag ttaaatcttt gtatattaaa tatctgtcta ttcttaagca
     7981 tgatccttct gttaatagct tctacaaaag taataaatac ccataatgaa gccgaaaata
     8041 attctcttaa atattgggaa agaactacta atttatacaa aacaaatatt actaatcaat
     8101 taaatagaaa caacaccgta gaagaaaata
//
LOCUS       DACETN010000100         7655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DACETN010000100 DACETN010000000
VERSION     DACETN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.104"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXF98_002272"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXF98_002272"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1556568.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IXF98_002273"
     CDS             complement(2032..3273)
                     /locus_tag="IXF98_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1556569.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IXF98_002274"
     CDS             complement(3291..4214)
                     /locus_tag="IXF98_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1556570.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IXF98_002275"
     CDS             complement(4435..6927)
                     /locus_tag="IXF98_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1556571.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IXF98_002276"
     CDS             complement(6934..7401)
                     /locus_tag="IXF98_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1556572.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IXF98_002277"
     tRNA            complement(7537..7610)
                     /locus_tag="IXF98_002277"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
BASE COUNT         2120 a         1665 c         1228 g         2642 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagca
//
LOCUS       DACETN010000101         7623 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DACETN010000101 DACETN010000000
VERSION     DACETN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.105"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(14..706)
                     /locus_tag="IXF98_002278"
     CDS             complement(14..706)
                     /locus_tag="IXF98_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1556573.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IXF98_002279"
                     /pseudo
     CDS             791..967
                     /locus_tag="IXF98_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IXF98_002280"
     CDS             complement(1143..1712)
                     /locus_tag="IXF98_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1556574.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IXF98_002281"
     CDS             complement(1786..2109)
                     /locus_tag="IXF98_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556575.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IXF98_002282"
     CDS             complement(2263..3075)
                     /locus_tag="IXF98_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556576.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IXF98_002283"
     CDS             3429..4277
                     /locus_tag="IXF98_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1556577.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IXF98_002284"
     CDS             4422..5363
                     /locus_tag="IXF98_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1556578.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IXF98_002285"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IXF98_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DACETN010000102         7598 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DACETN010000102 DACETN010000000
VERSION     DACETN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.106"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(136..426)
                     /locus_tag="IXF98_002286"
     CDS             complement(136..426)
                     /locus_tag="IXF98_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556579.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IXF98_002287"
     CDS             complement(544..804)
                     /locus_tag="IXF98_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556580.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IXF98_002288"
     CDS             complement(941..1354)
                     /locus_tag="IXF98_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1556581.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IXF98_002289"
     CDS             complement(1810..2295)
                     /locus_tag="IXF98_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556582.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IXF98_002290"
     CDS             complement(2434..2649)
                     /locus_tag="IXF98_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1556583.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IXF98_002291"
     CDS             2873..3673
                     /locus_tag="IXF98_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1556584.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IXF98_002292"
     CDS             3692..5560
                     /locus_tag="IXF98_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1556585.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLKLAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IXF98_002293"
     CDS             5553..6044
                     /locus_tag="IXF98_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1556586.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IXF98_002294"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IXF98_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1556587.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IXF98_002295"
     CDS             6604..>7598
                     /locus_tag="IXF98_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1556588.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgaa
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACETN010000103         7550 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DACETN010000103 DACETN010000000
VERSION     DACETN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.107"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(93..1196)
                     /locus_tag="IXF98_002296"
     CDS             complement(93..1196)
                     /locus_tag="IXF98_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1556589.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1286..2221)
                     /locus_tag="IXF98_002297"
     CDS             complement(1286..2221)
                     /locus_tag="IXF98_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1556590.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2523..4457
                     /locus_tag="IXF98_002298"
     CDS             2523..4457
                     /locus_tag="IXF98_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1556591.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4914..5330
                     /locus_tag="IXF98_002299"
     CDS             4914..5330
                     /locus_tag="IXF98_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1556592.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5344..5910
                     /locus_tag="IXF98_002300"
     CDS             5344..5910
                     /locus_tag="IXF98_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1556593.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            5933..6919
                     /locus_tag="IXF98_002301"
     CDS             5933..6919
                     /locus_tag="IXF98_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1556594.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7074..>7550)
                     /gene="scm"
                     /locus_tag="IXF98_002302"
     CDS             complement(7074..>7550)
                     /gene="scm"
                     /locus_tag="IXF98_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1556595.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2148 a         1452 c         1562 g         2388 t
ORIGIN      
        1 aataccccta ccgcaaaagg aaatacacta gccacctttg ttcgtatttc aactacttct
       61 aaaaagattt ttagtgtcat ggttcgtact ccttatttta gttcagaacg gtcacccatc
      121 gtgtaatggg agtcctccga taatttgaag gtgtctttga ttggatcggt cacatatact
      181 ttatcgtctt ttgtcacgaa gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat
      241 tccagtcctt ttttcacacc cattgaaaag actgctgtag acaacccgtc accatcaatt
      301 gatttatctg ttatgatcgt gacacttgcg atatcattgt cgaacggata acccgttttt
      361 ggatcaaaca aatgatggta ttttttgcca tctacttcta aataacgctc gtaaattcca
      421 gaggtaacta atgttttgtt ccgttcttta atagaaccta aaacagatcc gcgagccatg
      481 ttcggatctt ggatgccgac tgtccagtct tggttttcgc ctcttgggct gtggcctaag
      541 acatagacat taccgcccaa gtcaacgatg gcagtggtca ctccttgttt tttcaagact
      601 ttcactactt cgtccgtaat gtaacctttg gcgattgcac ctaaatcgat gatcattcct
      661 ttttctttta gataaacagt tttttctttg tcatttagtt cgatcttatg ataatccact
      721 aatttcaatg cctgatcgat ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt
      781 cgccataatt gtgtgattgc accaatcgcc atatcgaatc cgccttgaga atcttggcta
      841 tattcgtagg cccgcttgac taacgtgtag acgtcatcag aaacttttac tggtttgatc
      901 ccagcttgct cattcacttc atcgatctca gaaccttttt gattgatcgt gattttatca
      961 ccaagttctt ttactcgatc gaaggcaggt ttcaatgctg attctttgcc ttcatcatac
     1021 acacggatac gaacgtaagt ccctaacaag aactgttcat cagaatatgg atctttcaat
     1081 atcttggtag ctgctgaact ttcagtggtt gttttatttg ctgagttgct gcagccagtt
     1141 agcaccataa aagagatgcc cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt
     1201 ttcctctctt cattttgaaa ggtgagacga ctgcctaatc tggctatcgt cccaccctcc
     1261 aacttttatt agtatgatta tgtgattatt tttctgttac gatattatct acttcgattg
     1321 tttgagtgtc gcctttttca gctgcattca ctaattgttg tgcgtacatg atgaatgaat
     1381 gtgagctgtg tgtagcacct gtaacaactt ctacattagc aggtgagcca tcttcttcgc
     1441 ccattgcttt gacgaattcg tcgttcagag tagggatgta agtttttggt tctgtaccgc
     1501 tttttgcttt catattttca ttatattctg tatcatcttg ttttgattta ccgtctttgt
     1561 cgatataatc aaagtttgat tcagttactt tgccgccagc aactgtcatt tcgaattgca
     1621 cacggtagcc gttagagtag ttcttttctt caagcttgta agtgccgtct ttcaaatcag
     1681 caccattgtc gatttcgatt gtatctgtgt taccagcttg agcagcttgg atcaattgtt
     1741 gtgcgtagtt ttggaagctt tcagatgagt gagtagctcc agttacgact tccacaccgc
     1801 ttgcgctttg tgcttttaag aattggtcat tcaactcagg aatgtatttt tctgggtttg
     1861 tgccagattt tgcttccatg tttttgttgt attctgcatc ttttgttttt gattcgccgt
     1921 tttcattgac gttatcatat tttgattcag tgattttgcc gtcttttaca accatttcaa
     1981 agactgcacg gtaaccattt gaataatttt tttcttctaa tttatatgtg ccgtcttgca
     2041 aatctccgcc agctacaact tttgcagatg attctgttgt gctttttgca gtcgaagatt
     2101 gtgctgtaga tgattcagaa gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc
     2161 ctaacactag agctgaaaat gctaatacgg caaatccagt tacaaaacgt tttttcttca
     2221 ttttaatacc ccaccattcc atttctagtc cacaattttt ctttgtgatt cattatacat
     2281 tctaatcttt ttagcctata agtcaactat ttttgataaa taacccatgt aatatttcaa
     2341 acgttttctt tcagcaaatt tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc
     2401 aaaaggagtt agacaaaaga acagaacatc tatataatat atggagattg tgtaataagt
     2461 atcaaactaa aagaattatc atatattcat tatatagata gtctataagg agcggatgat
     2521 caatggcaaa gcaaaatatt gtcgttgtag gagcaggata tgccggtgtt tcagctacaa
     2581 aatacttggc aaagaaactg aaaaaagaag atgtagcaat tactctgatc gatcgtcatt
     2641 cgtaccatac tatgatgacc gagttgcatg aagtagctgg cggacgtgta gaaccagaag
     2701 caatccaata cgatctgcag catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag
     2761 acactgtaat cggtatcgat aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc
     2821 cttttgatca gctgatcctt ggaatgggcg gagagccgaa tgactttggt acacctggtg
     2881 tcaaagcaca cggattcacc ctttggtcat ttgaagattc tgtaaggatt cgggaacata
     2941 ttgagaagac agtagcaaaa gctgcattgg aaccagatga tgaactgcgt aaagccatgc
     3001 tgactttcgt tgtctgtggt tctggattta ccggtattga aatgattggg gaactgatcg
     3061 actggaaaaa tcgcctagcc aaagattata aattagatcc aaacgaaatc acactgatgg
     3121 tcgtagaggc aatgccgacg atcttgaata tgttggatcg aaatgatgct gcaaaagctg
     3181 aacgctattt gcgtaagcac aacgtggaat tattattgaa tgcaccaatc gtagaagtgg
     3241 ctgctgatca tatcaaattg aaagacggac gcaacattcc gactcacaca ctgatttgga
     3301 cagctggtgt caaaggaact tctgacgctg ctgactttgg attagaatca gcacgcggac
     3361 agcggttgat cgccaatgaa tatatgcagg caaaaggcta cgaagataaa aacatctata
     3421 tcattggtga tttagtcttt tatgaagaat tcccaggaac accgacaccg cagatcgttc
     3481 aagctgctga acaaacagga cacacagccg cagcgaatat cgtggcttcg atcaaaggcg
     3541 gagaaaagca taaatttaaa ggaaactatc aaggatttat ggtttctatt ggtgccaaat
     3601 ggggggtagc taacctattt gataagatcc atttaagcgg atttttagct atcgtaatga
     3661 aacatatcgt gaatttgaaa tacttctttg atattcgttc tggttattat atgttccaat
     3721 atttaatgca tgagtttttc catatcaaag atgatcgcaa cgtgactcga ggacattctt
     3781 ctcgttacgg caatgtctta tggagtgtgc cacttcgtat tttctatggt ttagtttggt
     3841 tcgttgaagc ttccaaaaaa atcattggga atggtgaagt gttcaaacca agtacttggt
     3901 ttggtgaagg atcatggttc actggaaaca gtgtgttccc ttggacatgg gaatacaatg
     3961 taacagatgt tgccagtggt gcttctgctg ctggtgatgc aaccacagct gccagcggag
     4021 caggggcaag tgccggagaa gcagcgactc aagctgcaca ttttggtttg agttatgcat
     4081 acggtgaaga accaatggct gtgattgaga agtcaccaga ctggttcaca aagatcatgc
     4141 aagtgatcat gccaaacaat gatgtagcaa tgttcttcca aaaattcatg gtattctttg
     4201 aaattgcctt ggcattagca ttgattgctg gattattcac atggctatgc agtgcgacaa
     4261 cgattgtatt ggtgatcacg ttctgcttat caggcatgtt ttactgggta aacatttggt
     4321 tcatttttgt tgctttcgca ttgatgaacg gttccggacg tgcgattggg ttggatcgct
     4381 gggtaattcc atggctacaa cgtacactag gtaattggtg gtacggtaga ccaagttccc
     4441 agtacggcaa gaaataaata gataaagaac gttatttgaa tgcaagattt caatagaaag
     4501 gggctgagac aaaccgtcac agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg
     4561 agataagcct ccatgagcaa gacacaggtg tcacaacctc tgttacaacc tcttgttata
     4621 aacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaacttgaaa
     4681 agtaattttc gtaaattcct tcttatttct tgaagttgcc cacttctgtc acaacctctt
     4741 gttataaacg gtgttccacc tgctgcttct cggtaataag cccaaagaat tagaaaacat
     4801 ggaaagctgt tttctattct tcgttcttat tactcaaagc aagacacagg tgtcacaacc
     4861 tcttgttttt atgctaaaat tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc
     4921 ttaaagaatt tgctgaaaaa agtctgctca aaccttggga tgtttttatt attttgcttt
     4981 tgactgttct ttctttttta ccagtcatga tttttggtct tcagcaaaac accgcaacag
     5041 cagaaaaaga agcggtactg cgtgtggatg gcacagaaat caaaacattc cctttgatgg
     5101 ctggagaaga gagcaacaca tatacttaca aagatgaaca cggagattat aacttgatcg
     5161 agatagatgg cgatcgagta cggataaaag aagcagattg cgatgaccag atctgcgtac
     5221 gtcgaggctg ggcaacgaaa aatggcgaaa ccatcgtctg tttgccacat aaattagtga
     5281 tcgaagtccg ttcaacagat ggaggtgacg aagacagtct gatttattag accttcacac
     5341 accatgagta aattacaaaa aatgatttat atttcgctac ttgttgcaca aggagtgatc
     5401 attggtttat tggaaaatat gatcccttat cctttttctt ttgcgcctgg tgcaaaacta
     5461 ggcttagcca atctaatcac gatcgttgcc atttttacca tgaaaaaaca agacagcttc
     5521 ttactacttt ggctacgact attcctgact actttgctag gcggaacgat ttccacattc
     5581 ctttatagta tgagtggtgc tttgcttagt tattttggaa tgttattggt caagcaatta
     5641 ggaccaaaac gtgtcagtat catcggaatt agtgcggccg gcggtttcat gcataatgtc
     5701 ggacagcttt tgatggcttc tttcattgca caatcatgga cagtcatgtt gtacttgcca
     5761 gtcctttcag ttttagggat tttatcaggg attgcaatcg ggatcgcagc gaattatctt
     5821 ttacgacatg tgcagacact tcaacgattc caaaaagatt atgagcaggg aaccaaacaa
     5881 aaatggagcc caatgtttac caaaaaataa tcgtcacaca aggagttttt ttatgaagct
     5941 acatccaatg tgggaaaact atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg
     6001 gatggaagaa actgtccgcc tgaaaaacaa acctgtcgaa caagcaatca aagaaacgat
     6061 ccatgccggc ggaaagctgt tgcgtcctgc ctatcagcta ttgttctcac aatttggacc
     6121 agagcaagat cgagacaaag ccatcgcttt ggcagcttct atcgaaatgc tccatacggc
     6181 cacactgatc catgatgata tcgttgatga agcggatctg cgacgtggtc agcctaatat
     6241 ccgtagtcag tttggtaatg tcgtttctgt ctatgcagga gactatttat ttgtctgctg
     6301 ttttaaacta ttgtctgatt actccacttc cttgaaaagt ctccaattga attctcgcag
     6361 tatggaaaaa gtattgaatg gcgaattagg gcagatggat gatcgctata attatgaact
     6421 ttctgtaaaa gaatatttgg aaaatatttc aggtaagaca gctgaactgt tccaattaag
     6481 ttgttctgtt ggcgcttttg aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga
     6541 tatcggactt tctatcggga tggcctttca gattatcgat gatattttag attatacaaa
     6601 agagagccag gaaatcggca aacctgtact agaagacatg cgtcaagggg tctattcttt
     6661 accgttgatc tactctttgc aaaaaaacaa accaaagcta ctaccttatt tagagaagaa
     6721 agcggcaatg acagaagaag atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg
     6781 cgtggaagaa gctaggaagt tagcggcctc ttacacagaa aaggctttga aggaaataaa
     6841 gaaattacct gccacctctc ttcgaacaaa agagaatctc ttttccctta cccaactgat
     6901 tttggatcgc aaagactgaa tagggaaagg ttcgatacgt aaataaattt ctcagaagga
     6961 aatctacgtg ttttttgaca aattgaattg tagttcttgt atttatgata aaaagcaaaa
     7021 ataaagagca acgtccgtta gcgacgttgc tcttttttat cttgaatatt tttttacctt
     7081 ttcgatttct tataactcat aaaagtgatt cttaaaccga ttacaagtaa tataacgcct
     7141 gcgagtgtta tccaattatt cgattgactt ccagttttag gaagtgctct ttttgatttg
     7201 tttccattgt cattgcttgt tttattagtt aagttaaagt tcgtcctgtt atccgaactt
     7261 gtttttgaga aggagacact tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta
     7321 ctatcgcttg aagttgatga atctgagcta tcacttgtgg tagaggatgt atctgtactg
     7381 tcacttgaag ctgacgtgtc cgtgctatca cttgtagtgg tagatgaatc tgagctatca
     7441 cttgtggtag aggtagtatc tgtactgtca cttgaagctg acgtgtccgt gctatcactt
     7501 gttgtggtag atgtatccgt actgtcgctt gaagctgacg tgtccgtgct
//
LOCUS       DACETN010000104         7396 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DACETN010000104 DACETN010000000
VERSION     DACETN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.108"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            161..391
                     /locus_tag="IXF98_002303"
     CDS             161..391
                     /locus_tag="IXF98_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1556596.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IXF98_002304"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IXF98_002304"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1556597.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IXF98_002305"
     CDS             1802..1978
                     /locus_tag="IXF98_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1556598.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IXF98_002306"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IXF98_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1556599.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IXF98_002307"
     CDS             2593..3060
                     /locus_tag="IXF98_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556600.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IXF98_002308"
     CDS             3145..4029
                     /locus_tag="IXF98_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1556601.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IXF98_002309"
     CDS             4146..4379
                     /locus_tag="IXF98_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1556602.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IXF98_002310"
     tRNA            4467..4547
                     /locus_tag="IXF98_002310"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IXF98_002311"
     tRNA            4554..4625
                     /locus_tag="IXF98_002311"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IXF98_002312"
     CDS             5140..5847
                     /locus_tag="IXF98_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1556603.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IXF98_002313"
     CDS             complement(5907..6551)
                     /locus_tag="IXF98_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1556604.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IXF98_002314"
     CDS             6678..6965
                     /locus_tag="IXF98_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556605.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IXF98_002315"
     CDS             7073..7393
                     /locus_tag="IXF98_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1556606.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
BASE COUNT         2450 a         1188 c         1593 g         2165 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaata
//
LOCUS       DACETN010000105         7337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DACETN010000105 DACETN010000000
VERSION     DACETN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.109"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            156..1634
                     /locus_tag="IXF98_002316"
     CDS             156..1634
                     /locus_tag="IXF98_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1556607.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1911..3971
                     /locus_tag="IXF98_002317"
     CDS             1911..3971
                     /locus_tag="IXF98_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556608.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4054..4623
                     /locus_tag="IXF98_002318"
     CDS             4054..4623
                     /locus_tag="IXF98_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556609.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4798..5604)
                     /locus_tag="IXF98_002319"
     CDS             complement(4798..5604)
                     /locus_tag="IXF98_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556610.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5742..7256
                     /locus_tag="IXF98_002320"
     CDS             5742..7256
                     /locus_tag="IXF98_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1556611.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2635 a         1206 c         1432 g         2064 t
ORIGIN      
        1 tccatttttt gcaatattct caaaaaaaag ataaaaaatg agaataactg atgaaaaaac
       61 aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa
      121 gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag
      181 aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg
      241 tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata
      301 tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag
      361 aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa
      421 tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa
      481 cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa
      541 gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg
      601 gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt
      661 tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg
      721 aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca
      781 ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg
      841 aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc
      901 ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca
      961 cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata
     1021 tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg
     1081 aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg
     1141 tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc
     1201 tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag
     1261 agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt
     1321 atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct
     1381 gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat
     1441 ttaaaaatta caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat
     1501 ctgattgttt acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc
     1561 cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga catttaactg
     1621 aaaaaatcag ctagtattta tggatggcag ctttgtctca catatttatt agctgattta
     1681 tgtttttttc aaagaaaagg taactagata taactagata taactagata tattacttaa
     1741 caaaattaaa aagtacataa gttattattc cgattttctt gaaacaataa aaaatcaaaa
     1801 aaaatactct cctaatagtc catcttcttt tgattttatt tatataatga aatcatctta
     1861 tgaaaattac tacttcatgt ttagacaaga gtagggagga ctaagaataa atgtgttcaa
     1921 aaagacgttc aaaacaacta aataaacacg cttgtcgttt agataaggaa cggtcgtgtt
     1981 ctgaaaaaca ctacgttaag tttatgatgc gcacgatgct gatcagttta tctgtaggtg
     2041 tctatatgca tgtacccgtt caggcaacga cacaaaccat caaggtcgta cgtacctctg
     2101 tttctgagac cactgattcc cctatcacta ttactcatac tttcactgat gcaaatggaa
     2161 aacagctact tcctgcattg acttcctata ataaagtcag ttatcctcca acgataaacc
     2221 actactggtt aattcctaac aaaacattat ggacatcgga aaccggaagc agtgtgccgc
     2281 tttctatctt ttccgatatg actgatccta caaaaggtga tcagttaaaa cagatcactg
     2341 atattttgaa tcatgctttg acagatataa aggacggtgg aagcgcacaa gtttcttatg
     2401 tatatgaaaa agattattca cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa
     2461 aagtttcgat atctgatctg gtagaaaggc tgaagaacgc agatggaaca caaggagata
     2521 tgtccaaagt aatttcaaac aatgggaata tcgtggatac gactaataca ggaacatcta
     2581 ctatttcctt gtcctatttt gatccaattg ctttaaaaac aatgactgca tctgctgtga
     2641 tccatgttcg tgctgatcaa acagctatca aaggaaaaga tcttcactta agaacaggag
     2701 agagttacgc ccctaaagat cttgttgaat atgtaacaaa aagcgatgga acttcaggaa
     2761 ataaagaaga aatcaaaatg aacgggacag tagatactac aactccggga gaatatcccg
     2821 ttgtattaac ttatatagac tcgaaaacat caaatgtggc agaaacaaca gctgtagttt
     2881 atgtagaagc atctgctgct tcatcgactg tcatcactca ttttatagat agtcaaacaa
     2941 ataaagaact tcaagcagaa acacatatga gtagtgtaaa tcgtattcct gatttgcgcc
     3001 tacctgctga attttctgat cgagtgccaa atatagattt atcgacttat gaacaaaatg
     3061 ggaaagtcac ttctatttct gattggatgc aagcaaacgg attagacaga aaaagtcact
     3121 ggtcggagat ccttgaagta cttcactcag aactgatgaa taataggaag caatcaggag
     3181 gggaaattac ctttacttat gtttatatac cagacaatcg ttcattagaa ggtcatgatc
     3241 ttattgttca tccaaacgaa aaaataaaca aagaagagtt gatccaatca gcaacaaatt
     3301 ccttaggtaa acccgtaccg ttaaaggatg tatcggttac aatcaatgaa gtaccattgg
     3361 aggatggtta tttgaccaaa caaacaggtg attttacgat tatgtatagt tatcatgacc
     3421 cgtattcact ccaagaactc catgctcagt cactattaca tgttttacca ttaaacaaac
     3481 agatagatca aaaagaacaa aaaagacaag aagaaagctt gtcagaaaaa aaacaacaaa
     3541 tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta gagttagcaa
     3601 gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct catttggccc
     3661 cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa caatcagaaa
     3721 aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag aaggaaacga
     3781 agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg caagtaccac
     3841 ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct ttaggagatt
     3901 tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt gacgcagatg
     3961 actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag tcatatgtta
     4021 tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt accttacgca
     4081 gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt agcggaaggt
     4141 tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat tgagacttcc
     4201 gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa ctttgctcct
     4261 tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg agaaaaagta
     4321 gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt ttttcattgg
     4381 ttagcagtga aaccagatta ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc
     4441 ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac acagacatgg
     4501 agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc tgaaaatcta
     4561 gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa attagataaa
     4621 taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag tcattcctca
     4681 tatcttactt ttttgagtta atactatcgg ataactaatg gcatgccata cattcaaaaa
     4741 agaaggggca gtgacaaaag tattgtcact gccccttctc ttgtttttta gcatgtttta
     4801 tcgtttgatt tttttaggaa taaaattctt gatgaacgta aatttttggt aatcgtttcg
     4861 actatagagg taaccaaata cactgaaaac aactgctcca ataaaattaa caaataaatc
     4921 cttcatagta tccatgattc ccacgtctaa gtaaccattt tggatgattg ttttatctgt
     4981 tttaccatct gcattttgac tttcaatgat tgtccgagtg atatctggaa catgatagac
     5041 agtgtttccg tctttactta actccacact attgatttgc tggatgatcg tatctttttg
     5101 catatcgaat tgcagtaatt tatctgctga aaattccaca aactcccaca taacaccaac
     5161 tgtcatcgaa aaacagaagg aaacaactgc tagtaataaa ggagtcaagt tcatggtttt
     5221 tgtattttta ttgagcaaat aaacaaggga aaagccgaca cccccagcta aaaatccgct
     5281 gaatgcatgt agaaaagtat cccacattga aaaatagcca taaaaatcac ttaactctcc
     5341 taaaactgtt gaactaaaaa taaaagataa caaaatgatt tctagcatat ttggaatctg
     5401 tatctttaat atcttttcca gaaaataagg gatttcaaat aaaattaatg tcagtaataa
     5461 aagcattgta tttccccagt gttggttcca gacctgatcc actagcataa taacgacgat
     5521 gatacgtaat aaaatataga ttccgtttgt gattctacct gcagtttttg tactgttcgt
     5581 aaaaaaggtt ttcaaatgat tcataaaata actccttcat gtatatcctt aattatacta
     5641 agaagcgtgt gtttaccaaa gctatatgac ttaaagaaaa acagagttga tattgtgaaa
     5701 aaaatcactt aatatagtat aaggagaaaa gggggataca aatgaaaaaa ttagttgctg
     5761 gtttattaac agccgttgca cttcttgcta tgagcgcatg cggcgcagat cagaaaaata
     5821 cagcgcagac aagttctagt acgaaggaat ctgccgaagt atcatcaggc gcttctgaac
     5881 aagtatatac agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt attggagctg
     5941 gtggtgcagg gatggcagca gctatcgaag caaaagatgc tgggttgaat ccagtaattt
     6001 tggaaaagat gcctgttgcc ggaggaaaca catctaaatc atcaagcggt atgaatgctt
     6061 cagaaacaaa atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag
     6121 aaacgctaaa aggtggtgga ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc
     6181 actctgctga tgcggtcgat tggctagatc aaaacggtat caaactggac aacttaacga
     6241 tcactggagg gatgagtgaa aagaggacac atcgtccgtc tgatggctcg gctattggtg
     6301 gatacttagt ggaggggtta ttgaataacg tccatgagcg ggaaatccct atttttgtca
     6361 acgcagatgt gacagacatt aagaaaaatg gggaaacggt caatgaagtg actgtacatg
     6421 ttcaaggaga agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt
     6481 ttggtgccag caaagaattt attgaaaaat atcgtcccga tttagctgat tatattacga
     6541 caaatcaaga aggatcaact ggagatggta tcaccatgat tgaaaaatta ggtggacaaa
     6601 ccgttgatat ggataaaata caaattcatc caactgtgca gcaggatgag ggggtcttga
     6661 ttggtgaggt tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg aaaagatttg
     6721 tcaatgaaat ggatacaagg gataaagtat ctgcagcaat tacagcattg cctgaaaaat
     6781 cagcttattt gatttttgac caaggcgtgc gtgatcgtgc cacagctatt gaattttatg
     6841 accaaaaagg atatgtcaca actggaaaga caatggaaga attagcagaa aaaatcgatt
     6901 taccaaaaga aacactagca aaaacagttg atggatggaa tacggatgtt gcggagaaaa
     6961 aagatgctca attcaaccgt gccacagcca tggaacatga cttatcgtct ccgaattatt
     7021 atgcgatcaa aatcgcacca ggaatccatt acacgatggg cggtgtgaaa atcaatacga
     7081 acactgaagt attagataaa gaaaaccagc caattagggg gttatatgct gctggtgaag
     7141 tcacaggtgg cttacatgga aacaaccgga tcggtggaaa ttcagttgcg gagatcatta
     7201 tttttgggcg acaagcgggc atacaagcag ctcgatttgc ttcggaaaat gaatgaaata
     7261 gaacgtaaat aaaaagaggt tgtgaaatca gcgttttgag ctaataccga gggtcagctg
     7321 attgaacact gtttagt
//
LOCUS       DACETN010000106         7187 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DACETN010000106 DACETN010000000
VERSION     DACETN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.110"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXF98_002321"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXF98_002321"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1556612.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IXF98_002322"
     CDS             complement(1172..1867)
                     /locus_tag="IXF98_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1556613.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IXF98_002323"
     CDS             complement(1880..2596)
                     /locus_tag="IXF98_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1556614.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IXF98_002324"
     CDS             2926..3534
                     /locus_tag="IXF98_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1556615.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IXF98_002325"
     CDS             3859..4182
                     /locus_tag="IXF98_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1556616.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IXF98_002326"
     CDS             4364..5734
                     /locus_tag="IXF98_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1556617.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IXF98_002327"
     CDS             6081..>7187
                     /locus_tag="IXF98_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1556618.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACETN010000107         6892 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DACETN010000107 DACETN010000000
VERSION     DACETN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.111"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..227
                     /locus_tag="IXF98_002328"
     CDS             <1..227
                     /locus_tag="IXF98_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAQ1556619.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="IXF98_002329"
     CDS             227..574
                     /locus_tag="IXF98_002329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556620.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="IXF98_002330"
     CDS             571..1620
                     /locus_tag="IXF98_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1556621.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="IXF98_002331"
     CDS             1635..1841
                     /locus_tag="IXF98_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556622.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="IXF98_002332"
     CDS             1862..2458
                     /locus_tag="IXF98_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAQ1556623.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="IXF98_002333"
     CDS             3410..4009
                     /locus_tag="IXF98_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1556624.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="IXF98_002334"
     CDS             4058..4774
                     /locus_tag="IXF98_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAQ1556625.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="IXF98_002335"
     CDS             4788..5567
                     /locus_tag="IXF98_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1556626.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="IXF98_002336"
     CDS             5650..6006
                     /locus_tag="IXF98_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1556627.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="IXF98_002337"
     CDS             5996..6262
                     /locus_tag="IXF98_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556628.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="IXF98_002338"
     CDS             6421..6699
                     /locus_tag="IXF98_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556629.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6892)
                     /locus_tag="IXF98_002339"
     CDS             complement(6696..>6892)
                     /locus_tag="IXF98_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1556630.1"
                     /translation="NNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKE
                     GTLFGFSVCTEIRILLGIPA"
BASE COUNT         2534 a          959 c         1335 g         2064 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatga tcttgttcta ttaaattgtt aa
//
LOCUS       DACETN010000108         6812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DACETN010000108 DACETN010000000
VERSION     DACETN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.112"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..152)
                     /locus_tag="IXF98_002340"
     CDS             complement(<1..152)
                     /locus_tag="IXF98_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1556631.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEIL"
     gene            complement(165..653)
                     /locus_tag="IXF98_002341"
     CDS             complement(165..653)
                     /locus_tag="IXF98_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1556632.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(619..1290)
                     /locus_tag="IXF98_002342"
     CDS             complement(619..1290)
                     /locus_tag="IXF98_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1556633.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            1428..2714
                     /locus_tag="IXF98_002343"
     CDS             1428..2714
                     /locus_tag="IXF98_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1556634.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2786..2980
                     /gene="ssrS"
                     /locus_tag="IXF98_002344"
     ncRNA           2786..2980
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXF98_002344"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3058..3342
                     /locus_tag="IXF98_002345"
     CDS             3058..3342
                     /locus_tag="IXF98_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556635.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            3415..3894
                     /locus_tag="IXF98_002346"
     CDS             3415..3894
                     /locus_tag="IXF98_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1556636.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            4117..4260
                     /locus_tag="IXF98_002347"
     CDS             4117..4260
                     /locus_tag="IXF98_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1556637.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            4838..6421
                     /locus_tag="IXF98_002348"
     CDS             4838..6421
                     /locus_tag="IXF98_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1556638.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
BASE COUNT         2091 a         1404 c         1357 g         1960 t
ORIGIN      
        1 tctaaaatct caccatattg atccgactga aaatgttcaa catctaaaga atcttcaatc
       61 gcgacaccac tcgcccctgc ctccatcaaa atattagcaa ctgcttcgac tgcttcactt
      121 tcagtttcta cttttacttc attccatttc atatctgtca ctccttaata acttgggtac
      181 ggtaaataag tatctgcttc attttgctgt tttacttcta ctaattctgc tagttgctga
      241 ttatcccagt gaagctcgaa tacagcttcg tcatcgtctt catccaaaaa atcaagcaaa
      301 tcgctttgcc cttgagctaa tacttcattt agatattcca ctaaagaatc agctactgat
      361 tttgccaacc ctttttttcc ttcataaggg attactgcta gataattgcg ttcatcaaaa
      421 acagatttct gaggattaaa aagaagtacg ccgtcttcaa attcgataat tggttcttgc
      481 gaaacgatcc cttctgcatc atcaatctca aattgtgctt tattttctgc aaacaagcga
      541 acaacgatct cgatcgtatg attttttgta tcccaatcta aagctacatc atagtctgtc
      601 acttgttttt caattatttc atctaaatat gttaatagat ccttcttttt cattttcttt
      661 cctccaaccc caatctttct cgatttggtt ttttctttgt aaagagatat aaggatttcc
      721 cgacacaaca aaacgcaacg gcttttctgt ccatacacct ttgtttggga taccgattct
      781 aggcaaagca ttgatctttt ttggtgtctt ttttttctca aaaactaagc gtaacgaact
      841 atctgctata gactgaccat aaagttcttg aggaattgct aatgcttcca ctaattttcc
      901 cggaccgttg ctgatatctg gaccagtttt tccgccacga tttttactca tttgatcaat
      961 cattgcggcg ggttcgattg cacgtatcat tactccttga ggaatccctt ctggctgagt
     1021 aatgatgttc aaaatccgat gagtatgcat cgtgtaaagg tagatcgtac ctggtttttc
     1081 atacattgcc cgcacacgcg gtgttcttct taaaccatag ctatgggcgg cctcgtcttc
     1141 tggacctagg tatgcttccg cgtctacaat atatccagct aatcttcctt ctggtgtgtc
     1201 atgctccaaa tacatcccca ataaatcccg tgccacttct ggcgtcgttt tattccaaaa
     1261 aaattcatct atcttttgtt tatccgtcat ttttctcacc tactttatca tacacaatat
     1321 tagttggata agctatgaaa acaaaacaac agaaggctgt tctttctagg catcttgcta
     1381 aatatgatat aattaaggga cgtaaaaaca agaaggagga aaatcctatg caacagccat
     1441 tggcttatcg catgagacct cgaacgatcg atgaagtcgt tggtcagcag catctagtcg
     1501 gggaaggaaa aatcatacgc cgcatggtcg atgctcgcat gctctcttcc atgattctat
     1561 atggacctcc tggtactgga aaaacgagta tcgcaagtgc gatcgccggc tctactaatt
     1621 atgcatttcg catgctaaac gccgctacag attcaaaaaa ggatctacaa gttgtagcgg
     1681 aagaagcaaa aatgagtggt acagtgatcc tcttattgga tgaagtacac cgattagata
     1741 aaacaaaaca ggattttctg cttcctcatt tagaaagtgg ccgaatcatc ttgatcggag
     1801 cgactactga gaatccttat attaccatca atccagcgat ccgcagccgt acacagatct
     1861 ttgaagtcaa accattaaca gaacaagaca tccagttggc agtcgaacat gctttaaggg
     1921 ataaagaacg cggccttgga caacaagcaa tccagttaga cgaagatgct cttcttcacc
     1981 tttcgcgtgc aacgaacggc gatttgcgga gtgcattgaa tgggctggaa ctggctacac
     2041 tatcgactcc ttcagataaa gaaggaagga ttcatcttac actgtcgatc attgaagaat
     2101 gtgtccaaag aaaagctctt acccatgaca aaaatggaga tgctcattat gacgttattt
     2161 cggcttttca aaaatcgatt cgtggaagtg atgtggatgc tgcacttcat tatctcgccc
     2221 gtttagttga ggcaggtgat ctggcaagta tttgccggcg tctgatggtt atcggttatg
     2281 aagatatagg tttaggtaat cctgctgctg cggctcgtac agtgaacgct gtattagctg
     2341 ctgaaaaatt gggattgcct gaggcacgta tcccactagc agacgtcgtc gtcgatcttt
     2401 gcttgtcgcc aaaatccaat tctgcctaca tggcattaga tgcagcactt gctgatattc
     2461 gagagggcaa agctggagat gttcctgatc atttgagaga tagccattac aaaggtgcga
     2521 aatcactcaa ccgtggcgtg ggttatcagt atcctcatca ttttgatcag gcttgggtca
     2581 atcagcaata tttaccagat aaactgaaga atgctcagta ttatcagcca aaagatacag
     2641 ggaaatatga acaggctctg ggtcaacagt attaccgtat aaaagagtgg aaaaagcatc
     2701 ctccaaaaaa ctagacaaga aaacggttct gtttctgcga gctctctcat tgctcttttt
     2761 aaattcatat gctactatag aggtgggaaa tctgtgatgt tcgaattgtc ctctttgtgt
     2821 gttgaccgaa catttttatt gatatcttgg gacccgtctt gtgtcactgc tggtaacatg
     2881 ccttatcatt tggtagttcg aagctacgac catgggaccc acctgcttga attcgcggga
     2941 tcaatactac aggacaaaca acggcattaa cggagattac catcttttaa agaataaagc
     3001 aatttgctct tggcaaattg ctttttctat ttgttgctta ttttggagga tttgttcatg
     3061 attaggattt taatgattat cgccacttta gtattattat tcgttagcta ttatttattt
     3121 aataaacagg atatcttttt tgtattgatc aacaaaaatg ataaaaatca aggattccta
     3181 caattttacg gagctgcata tgctgtttta ggcgtaatgg gtattttaac agcttttttc
     3241 aatcaacgct ttattgcctt ggtcttttta ctgatcgtaa ttattgtttc tgccaccttc
     3301 agtatccgat ttgcaaaaaa aattgctgaa ccaaagcagt aaataattct ctttttccct
     3361 aaagtgttct acaataaaag agtacaatac gattgacttg aggagtgaat gcttatgtta
     3421 caacaataca aaaaaatcat ggttgccgta gatggttctg atgaagctga attagctttt
     3481 aaaaaggcgg tcaatgtagc tatccgaaat aacggagaac ttctattagc acatgtcatt
     3541 gacacacgtt ctttccaaac cgtttcatcc tttgatggaa tgcttgcaga acaagctact
     3601 gaaatggcaa agcaaacatt ggcagattat gaaagcaatg cgaagaaagc tggcttgaat
     3661 aatgtaacct ctgtcgtaga atatggctca ccaaaacaaa ttattgccag agaaatccca
     3721 gaagataatc aagtagattt gattatgtta ggagccacag gattgaatgc tgtagaacgt
     3781 ttatttattg gttctgtttc tgaatatgtt attagaaacg cagcttgcga tgtgcttgtt
     3841 gtccgaactg atttagaaaa ccaattgcca gctaaagacc acaccaaagc ttaacacgta
     3901 gacgacctgc caactatcgg caggtttttt tgcacaaaaa accgtgataa aaacaaaaga
     3961 cgcggcaagc actcatcggt ctttataatg tttaaaataa agggaaaaca gtcaatgagt
     4021 tcaagtaaaa taaaaaaaag tgaacaccag ggcgattagt cgccctggtg tggtttaaaa
     4081 cgacctaagc atgccaaagc ttattggttc gtcaatgtgg aagtttccga tgaatctgat
     4141 acgaaaaaat atgaagtatc gatggcggat accgatttta tcgccaaact aaaattttat
     4201 ttcgggttcg ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt caagaattga
     4261 tgaaaggcta ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga tgttggtgag
     4321 atttccaaag agaagagctg tttaatgcaa cggtattaag caaatggcaa aaggaagtca
     4381 tgtagacaaa gttttttatc aaacaccata tgatttcgcc tgaacgctgg tttagctcag
     4441 aatacaatga tgtggtccac gagacattac cctcagtcag ataaaaaaag ctctctgaca
     4501 gaattacaca tcgataaata ttaagtaaat gtcagcgcta gttgaacttt ctgacacttt
     4561 caggtacact ggaaaagaca actacacgaa ggagggaaaa tactatgatg cgcatgacta
     4621 aaataacaag taatgaaaat taccaacgat accatatcgc atttagccat gcaattggtt
     4681 ttacagttgc tgttgacggg acaagtttgt ccaaaacagt tgaattcacg taaaatcaat
     4741 ggccatctca aggtattttt ctcatgataa tctgagttta aataacacag gccatggata
     4801 accatggcct ttttgagtgg tctcaaggag agagatcatg atcatacaag caatttctat
     4861 taaaaaaaca atcggcggga atccattatt tgaggattta tcctgtcaaa ttgaagctgg
     4921 tgaaaaaatt gccttagtcg gtatcaacgg aactggcaaa tcaacctttt tacaaatctt
     4981 gaccggcaga gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct taaagatcgg
     5041 gacggtcgaa caagaactta cagtttccga agcgaccgtc aaccactact tgctccattc
     5101 tgccgctgag atacaggacc tcaaacagca aatgagtcgc tacgaagcat tgatgacaga
     5161 accagataca aacttagaga agtgtctggc ccgttacggt gagctgcagc atcgctttga
     5221 agaactaggc ggttacgtat tagaagaccg gattgcagcg atcttgcaag gcttagggat
     5281 tccacaccgt caggaagcac ggttaacaga acttagcgga ggcgaacgag ttaaagtcgc
     5341 tttagccaaa attttagcag ccgatgccga tttactttta ctcgatgaac cgaccaatca
     5401 tttagatctt aacagtattc gctggttgga aaactattta cagaatacaa aaaaatcggt
     5461 tctggtcgtt tctcatgatc ggcaatttct cgatcaagtc accacaaaaa catgggaact
     5521 ggaagaaggt gcattgattg agtatcccgg aaactacagc cgttttcgag tcttgaaaga
     5581 agctcgcttg gctgagctga caaaaaatta cgaactgcag caaaaagaag tccagcggtt
     5641 gaaagtcatg attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg agtccttctt
     5701 caaaaaagcc aaagaactgg aacgccgcct ggcgaaactg acactcgtca aaccgccacc
     5761 tcccccgaaa aatcggctgc aaagcttgtc taatggtgga aaatctggca aagaagtttt
     5821 catcattcaa aacttgcacc agcagtacgc tgatcaagta ctattcaaag acagcagttt
     5881 tgcagtctat cgtggagatc atttagccat catcggcgac aatggtgcag gaaaatcaac
     5941 cttactcaaa ttattactcg gtgaggaaaa gccttacggt ggcaccatca aattaggtag
     6001 tagcttgcaa atcggttatt tgccgcagca gctgcagttt gccgatcccg atagccgttt
     6061 gctggcttac gccatcacat tgactggaaa cgaagaagtc gctcgacggc aattagccaa
     6121 aagtggcttt tatcaaagcg atgtgggtaa acggatcaaa gatttatccg gtggtgaaaa
     6181 aatccgtttg gcgttgctga aactcttttt agaaaaaatc aacgtcctga ttttggatga
     6241 accaaccaat cacctcgaca gctatgctcg agaagaaatt gaagaaatgc tgcaagacta
     6301 tcaaggaacg ctcctagcag tcagccatga ccgctacttt ttgcagcaac acttccaaga
     6361 agcactcgtt attgatcagg agcagatcag ccgccagccg ctcggccaac tgattgacta
     6421 ggacgttttt tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg attcaggcaa
     6481 agtacaggtg gaaaatcaag tttttattat tcgatcaatg aatgtttcgg atcggttttc
     6541 tagctaaaca taaagcgaag aataaagtcg acagcccacg aggctgatct aaaaatgttt
     6601 tttcttttta catatgtaat gtcccttata aagatgaatg aaaataaaat attgggattt
     6661 tcctgaaaat caataaataa aatgccagtc ttttcccttt tgaccacttt tccaagcaaa
     6721 attccctaag aagatcctgt aaaaaagaag ggaaattgaa gttaaattac cattttgcgg
     6781 tcttctccgc aaaaaaaaca catgcaagaa tt
//
LOCUS       DACETN010000109         6077 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DACETN010000109 DACETN010000000
VERSION     DACETN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.113"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IXF98_002349"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IXF98_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1556639.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IXF98_002350"
     CDS             complement(2203..4389)
                     /locus_tag="IXF98_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1556640.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IXF98_002351"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IXF98_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1556641.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IXF98_002352"
     CDS             complement(4611..5051)
                     /locus_tag="IXF98_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1556642.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IXF98_002353"
     CDS             complement(5125..5664)
                     /locus_tag="IXF98_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1556643.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1576 a         1368 c         1068 g         2065 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgagg
//
LOCUS       DACETN010000110         6069 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DACETN010000110 DACETN010000000
VERSION     DACETN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.114"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..134
                     /locus_tag="IXF98_002354"
     CDS             <1..134
                     /locus_tag="IXF98_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556644.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="IXF98_002355"
     CDS             complement(220..1314)
                     /locus_tag="IXF98_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1556645.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="IXF98_002356"
     CDS             1503..2264
                     /locus_tag="IXF98_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1556646.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IXF98_002357"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IXF98_002357"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1556647.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3039..3794
                     /gene="fabI"
                     /locus_tag="IXF98_002358"
     CDS             3039..3794
                     /gene="fabI"
                     /locus_tag="IXF98_002358"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1556648.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3904..4917)
                     /locus_tag="IXF98_002359"
     CDS             complement(3904..4917)
                     /locus_tag="IXF98_002359"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1556649.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5082..6002
                     /locus_tag="IXF98_002360"
     CDS             5082..6002
                     /locus_tag="IXF98_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1556650.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1863 a         1205 c         1081 g         1920 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tttcattact ttgactatca aagtatcata
     2881 ttcctaaata tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca
     2941 ttttgactaa taattaagaa atcaagaagt tattttagct attattctct atttttttac
     3001 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
     3061 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
     3121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
     3181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
     3241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
     3301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
     3361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
     3421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
     3481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
     3541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
     3601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
     3661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
     3721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
     3781 tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa
     3841 aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg
     3901 aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc
     3961 tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac
     4021 atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca
     4081 tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg
     4141 cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat
     4201 agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc
     4261 acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc
     4321 cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc
     4381 tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt
     4441 tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg
     4501 agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt
     4561 agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc
     4621 atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat
     4681 ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc
     4741 ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt
     4801 caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat
     4861 aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata
     4921 cttaccacct tcgttatttt ctccacattt tatcattatc catattattt gtcattttat
     4981 tttcagcttt tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg
     5041 gtacaataca gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa
     5101 ctgccggtac gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa
     5161 atgcgtttca gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc
     5221 aaaaaagttt atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa
     5281 aatatctgat tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc
     5341 gcttaaagac tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt
     5401 ttatctatgc aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg
     5461 gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg
     5521 gattttttat tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact
     5581 taacgattga aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg
     5641 atggtactgt ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg
     5701 caaatatgca agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct
     5761 atgagatcat tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa
     5821 ctgaaccttc tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg
     5881 tctgctacgc attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg
     5941 tcaaagaatt gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat
     6001 aatttggaca aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa agtaaagaac
     6061 acttttgtc
//
LOCUS       DACETN010000111         5873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DACETN010000111 DACETN010000000
VERSION     DACETN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.116"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            640..1374
                     /locus_tag="IXF98_002361"
     CDS             640..1374
                     /locus_tag="IXF98_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1556651.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="IXF98_002362"
     CDS             1390..2559
                     /locus_tag="IXF98_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1556652.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="IXF98_002363"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="IXF98_002363"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1556653.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="IXF98_002364"
     CDS             3649..4575
                     /locus_tag="IXF98_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1556654.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="IXF98_002365"
     CDS             4594..5844
                     /locus_tag="IXF98_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1556655.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1201 g         1750 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacaaca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag tta
//
LOCUS       DACETN010000112         5844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DACETN010000112 DACETN010000000
VERSION     DACETN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.118"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            49..309
                     /locus_tag="IXF98_002366"
                     /pseudo
     CDS             49..309
                     /locus_tag="IXF98_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            472..1137
                     /locus_tag="IXF98_002367"
                     /pseudo
     CDS             472..1137
                     /locus_tag="IXF98_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1173..1418
                     /locus_tag="IXF98_002368"
     CDS             1173..1418
                     /locus_tag="IXF98_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1556656.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1521..2390
                     /locus_tag="IXF98_002369"
     CDS             1521..2390
                     /locus_tag="IXF98_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1556657.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2371..3105
                     /locus_tag="IXF98_002370"
     CDS             2371..3105
                     /locus_tag="IXF98_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1556658.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3138..4046
                     /locus_tag="IXF98_002371"
     CDS             3138..4046
                     /locus_tag="IXF98_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1556659.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4043..4585
                     /gene="sat4"
                     /locus_tag="IXF98_002372"
     CDS             4043..4585
                     /gene="sat4"
                     /locus_tag="IXF98_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1556660.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4678..5472
                     /locus_tag="IXF98_002373"
                     /note="aph(3')-IIIa"
     CDS             4678..5472
                     /locus_tag="IXF98_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1556661.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5580..5768)
                     /locus_tag="IXF98_002374"
                     /pseudo
     CDS             complement(5580..5768)
                     /locus_tag="IXF98_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5748..>5844
                     /locus_tag="IXF98_002375"
     CDS             5748..>5844
                     /locus_tag="IXF98_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1556662.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNIS"
BASE COUNT         2021 a          867 c         1269 g         1687 t
ORIGIN      
        1 aatttgtatc gataaggggt acaaattccc actaagcgct cgggacccct tgtaggaaaa
       61 tgtcctaagt gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat
      121 cctgaatgta agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat
      181 gtaaaagaat tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa
      241 aagccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatctctttt
      301 tcaaaataaa catggggctg acccaaaagt agctcctata tgaaaaatag aggtctttcc
      361 atttagatag tagtggaaag acctctattt atcttttgaa agtgaataca aaaataaagc
      421 acactctgct ataatatagg tgattaatcc atttgagaga ggtgctttat catgtatcaa
      481 aattatacca taaaccaact ttgtttgcct atagatcttg aaattaaatt agaagaaaat
      541 gattttgctc atgccattgt gcagtttgtt gactctattc ccgatgaagt ctttcttcct
      601 tattatcaat ctatgggtag accccaatat catcctcgta tgctactttc cattattctt
      661 tgtgcctata ttcaaggggt ttattcgggc cgccaaatac aaaatatgct gatagatagt
      721 attcggatgc gctacttatc tcaagagcaa ttccctaact ttcgtactat taatcgtttt
      781 cgcgttcatc cgattatgaa cgatattcta gatcattcat ttgttcaatt tcgtgagcta
      841 ctggttcaaa gtggcttaat cagtggcagt gctctataca ttgatggtac aaaaattgaa
      901 gcggatgcga ataaatactc tttcgtttgg aaaaaaagta ttttaaagta taaggatagc
      961 cttgatcaaa aagcccttga aaactaccaa caaatggtgg aaacaaaaat cttacctgag
     1021 ttaatagatg atttaaagga tgaactttct atagaagaga ttgaaaggat tcgtcaatct
     1081 ttggaagaaa aggaaaatca attaattgaa gcgattgacc aaaccgaaac agtagaaggg
     1141 ggctgaccca ttattaataa ggagtggaca acatgattga tgtaaatgag ctattggaag
     1201 aagctattag agaaactgaa aatctaaatg atggcgaagt ttttcttgtt aaagatttat
     1261 tcaagggata tgtggggaat aggataccca gaaaggatcg acttctgctt ggaacgttat
     1321 ttttaaacca tgttagcaaa atgaatggta atttaaaggc tattgaaaag acttcatcta
     1381 atcagcagag gtataaaaag acaattgata agttttaact atcttttgtg aaattaaaaa
     1441 aattataaat atgttgagta gaagcaaaag atttggcttg gaatttttac agcagtgaat
     1501 cgataaaagg agcatgcttg atggtagata atattattaa atcagtagca gagaaattat
     1561 cctctctgtc ttatatagaa ggtattgttt taggtggttc acgtgcaagg ggcacccata
     1621 cagaggattc ggatatagat atcggcatct attacaattc agaatcattt gacataaata
     1681 ctattaatca attcgctaca aagctggatg atgagcatag aaataacctt gttgtacctc
     1741 ccggagcatg gggtgattgg attaatggcg gcggatggtt agtcataaac gggtatcatg
     1801 tggatttaat tttacgtgat attaaacgtg tggaacaaat aatgaaagat acagagcacg
     1861 gaattgttac tgccaattat cagactgggc atccccatgg ttatattagt gcaatgtatc
     1921 gaggagaatt agcgattagc aaaatactat atgctaagaa tgaaagctta tgcgaattaa
     1981 aaaaacaggc agaaacttat cccaatgctt tgcagaaaag tttagttaac ttttttatgt
     2041 ttgaagcagg gttctcttta atgtttgtaa aagcaaattc gggaacagac gataaatatt
     2101 atattgcggg tcatgttttt cgtatagttt catgtttaaa tcaagtgtta tttgcatgta
     2161 ataatgctta ttgtatcaac gaaaagaaag ctataaaact gcttgaaact tttgaacata
     2221 aacctgaaaa atataccgag aaggtaaatc atatttttga agtactcggt atctcacttt
     2281 ttgaatgcta cgacatgacc gagaagcttt ataatgaagt gaatgaaatt gtatcggaga
     2341 taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga tgataatata
     2401 ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa
     2461 tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct tgatttagga
     2521 tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt
     2581 gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt tccacagatt
     2641 gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt tgatgtaata
     2701 ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa aaagatatat
     2761 aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt ttttactgct
     2821 catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt cccggtggac
     2881 aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt tacaaaatat
     2941 catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat aataaatcag
     3001 attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc ggatgaaatg
     3061 cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata tagaaaaaat
     3121 aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag
     3181 cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     3241 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     3301 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     3361 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     3421 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     3481 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     3541 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     3601 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     3661 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     3721 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     3781 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     3841 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     3901 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     3961 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     4021 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     4081 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     4141 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     4201 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     4261 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     4321 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     4381 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     4441 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     4501 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     4561 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     4621 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     4681 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     4741 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     4801 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     4861 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     4921 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     4981 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     5041 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgattagcc
     5101 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     5161 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     5221 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     5281 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     5341 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     5401 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     5461 gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag ctttttagac
     5521 atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt atatcttttc
     5581 taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt gaagaggttt
     5641 gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc tagcttaaga
     5701 acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg aacaggatta
     5761 acagagtaac ctctattctt attcagctgc aatcaaaaaa aataattcct gccaaagaaa
     5821 ttgcacagcg atttaatata agct
//
LOCUS       DACETN010000113         5771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DACETN010000113 DACETN010000000
VERSION     DACETN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.119"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1016..1306)
                     /locus_tag="IXF98_002376"
     CDS             complement(1016..1306)
                     /locus_tag="IXF98_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556663.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1648..1944
                     /locus_tag="IXF98_002377"
     CDS             1648..1944
                     /locus_tag="IXF98_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556664.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="IXF98_002378"
     CDS             1951..3903
                     /locus_tag="IXF98_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1556665.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="IXF98_002379"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="IXF98_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1556666.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="IXF98_002380"
     CDS             complement(4799..5491)
                     /locus_tag="IXF98_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1556667.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1895 a          997 c          917 g         1962 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     5581 tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc
     5641 ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa
     5701 aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta ctgtatttat
     5761 tacaaaaaac g
//
LOCUS       DACETN010000114         5745 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DACETN010000114 DACETN010000000
VERSION     DACETN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.120"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(131..802)
                     /locus_tag="IXF98_002381"
     CDS             complement(131..802)
                     /locus_tag="IXF98_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556668.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(795..1289)
                     /locus_tag="IXF98_002382"
     CDS             complement(795..1289)
                     /locus_tag="IXF98_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1556669.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1309..2529)
                     /locus_tag="IXF98_002383"
     CDS             complement(1309..2529)
                     /locus_tag="IXF98_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556670.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2676..4511
                     /gene="lepA"
                     /locus_tag="IXF98_002384"
     CDS             2676..4511
                     /gene="lepA"
                     /locus_tag="IXF98_002384"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1556671.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4605..5732)
                     /locus_tag="IXF98_002385"
     CDS             complement(4605..5732)
                     /locus_tag="IXF98_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1556672.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1759 a         1154 c         1093 g         1739 t
ORIGIN      
        1 agaaaaaagg ggtctgacac aactagtgtc agacctcctt ttttcaaata aacagtgttc
       61 cagaagtagt ttcttcgaaa aataagccga aatttcttga agctgcccgc ttctgtcaca
      121 acctctttta ttaggtattt ttcacttttt ctaatttata actttcaaag taacgctcat
      181 cttttttact ttgagaccat gcaaacaatc ccattaaggc agcacctatc gtatttgtca
      241 gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac aaacagtgtg ccatcagaag
      301 cagcaaaacg ttgaagattc atgcctaaga actggtcgca taagaattcc cagaactccc
      361 aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa gaggaacaac caaggactgg
      421 tttttgatat ctcagcatct ttcatcaagt aaccaatcaa tccatagcca agagctgtca
      481 ggaccattgg actcaccgca tgtaagattt tgtcccaaaa agagatgata ctgatcaaat
      541 gcatccctgt gcctaaaaag acagagatga acaagaaaaa ccagtaaaac aaaacgattg
      601 ctgctggtat tttaagtttg aaaactttca aaatgacttg tggcatgaaa atcaatgcaa
      661 tccctaataa agattccacg ataaatgtga ttccttgaga agtcgaatat ttttgtgtga
      721 taaattcata aaaattatag cagacaacga atacaccaaa agcaagtaaa gctatttgca
      781 cctttttgat ttctttaatc actgaaagtc cctccatcta aaaatctttt tacttctgaa
      841 agcaactcaa tttctgaaga aaccactttt ccgttcaatt tgatcaaacc tattgtatat
      901 aagttcaggt agtgaaattg attttcagct gtttcttgta gtgcagcgat ttttttcggg
      961 tgggttccgc cttgctgtcg agtatcagta tataagccga tcactgggat gcctttcgca
     1021 taagccacac cgatttccga tgctaccccg gcatcgatcg tcagcccatc tagtaaagca
     1081 atcattaaat cactagccaa cactttttct gtgtcagcaa gagcaatcat tcgactgtct
     1141 gcataagcag ttttgtcatt gattgcttca ttttcttgag gaagatagat cgaaagctca
     1201 gaagagagct ctctgatttc tttaactaac tgttggttat ataacaaatc acttttagca
     1261 aacattggtg cggcaaaata aattttcatc taaacatcat cctttttatc aaaaataatc
     1321 gctaaagtaa gcttccatgt ttggaatgat acgctctagt gacttcagtg tttcttcatc
     1381 tgtttctaac cgttcgattc ttgccaaata agaagcacgg cgataattca tcatcaagca
     1441 agtcaatgtt tggatatcca gtcgaacgga ttttcctata ggttcattta agacacggac
     1501 ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta ttccaaggag cgacaggatc
     1561 ttcaatgatg aaatgaaaag catcagctgt tccaacaaac ggaaaccgtt gaaggaattc
     1621 ttttacatca acgatccgag ccataaaata tggttcgatc tgctctttga tctgactatc
     1681 ttcaatcaaa aatgctaaag gctcattttt ataaatatct ccatgtaccc agtagatcat
     1741 ggaaaaatgt gcggaaataa agttccataa gccatttcga gcttcttgat caagatagaa
     1801 catttctttt acgtgaaata tttcttcagc tacccagtag aacagaacac ctcttggttc
     1861 atggttgcta tcgtagtaga cagcggctgt gcgctcatct tcattttcga aacgccaata
     1921 ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg ttttgttggg caaatttgtc
     1981 ataaacggaa aatacgtctg gatgatcaac tggtaatcgc tcaaccatac cacttactgg
     2041 gatttgttta ggtagctgtg tatctctgat tttaaaggaa agtttatcag acatgatttc
     2101 ccatccttta cgccgatagt aggggatact gtaaggatac aaataagaga tccattgctt
     2161 attcttgcgc atattatcca atgcaacgat aattagatct tgcatcaatc catggttagc
     2221 atattctgga taagttccaa ctccagtcac accacccatt ttataacgtg caccgtgaat
     2281 attgacttca caaggataga tagcgatttg agagatcagt ttattttcgt ggaaccatcc
     2341 gaacactttc gatacttcca aaatcggttt tttggaacgg ataaaagctc gtttattctc
     2401 aaacccgctt tcctcgatgt ctgcttcagt cacttggaac acataggaaa gcaattcatt
     2461 gaattgatcg atatgttctt gagtgaccgg ttttaattca agattttctc taaaatcttt
     2521 ttgatccatt ttttcatctc catttattag cttgataaca gtatagcaaa attctacaaa
     2581 aaaaacttct atagctgact aattaaaaac tctttaaatt actgcttttc gttggtataa
     2641 tggaaagtgc tgattatatg aaaagaggag acgctatgga tataaagaaa atgaaagaac
     2701 gccaagagca gattcgtaat ttttcgatca tcgctcatat cgaccatggg aaatctacat
     2761 tggccgatcg tattttagaa atgacgcaca ctgtgacgtc tcgcgaaatg caagatcagc
     2821 tgttagactc gatggatttg gaacgtgaac gtggaatcac gattaaatta aatgctgtag
     2881 aactcaatta cacagcgaaa aatggagaaa catatatctt tcacttgatt gacaccccag
     2941 ggcacgtcga cttcacctat gaagtctctc gaagccttgc tgcctgtgag ggtgccgttt
     3001 tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt ggccaatgtt tatttagcgt
     3061 tagacaatga tttggagatc ctgccagtca tcaataaaat cgacctaccc gcagcagatc
     3121 ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat cgatgctagt gaagctgtat
     3181 tagccagtgc caaatcaggg attgggattg aagatattct cgagcagatc gtggaatatg
     3241 ttcctgcacc aagtggagac ttagatgcac cgctaaaagc tttgatcttt gactctattt
     3301 atgatagtta ccgcggtgtt gttctaaatg tccggatcac ggacggtatg gtcaaacccg
     3361 gagacaaaat caaactgatg agtaatggca aaacatttga tgttacagag gtcggggtct
     3421 tctcgccaaa agcagttgct agagactttt tgatggtcgg tgatgtcggt tatatcacag
     3481 caagtatcaa aactgtccaa gatacacgag taggggatac agtaacgtta gcagacaatc
     3541 ctgccgctga agctttacca ggttaccgaa aaatgaatcc gatggtttat tgtggtcttt
     3601 atccaattga tacttctcgt tacaacgatt tgcgggaagc tttagaaaaa ctgcaattga
     3661 atgatgctgc tttacaattc gaaccagaaa cctctcaagc attaggtttt gggttccgct
     3721 gtggattcct cggacttctt catatggatg tcgtccaaga acgcttggaa cgggaattca
     3781 atctagaatt gatcacgact gcaccatccg ttatttacca tgttaataaa acggatggta
     3841 cgactgctac agtagataac ccggcagatt ttcctgaacc tgtaacgatt caggatgtag
     3901 aagaaccatt cgtcaaagca cagatcatgg tcccaaatga ttttgtcggt gcagtaatgg
     3961 aactttctca acgaaaacgg ggagaattca ttacgatgga ttatttagat gattaccgag
     4021 taaatgtcgt ctataatatt ccgctttctg aaattgtctt tgactttttc gacaaattga
     4081 aatcaagcac gaaaggctat gcctcattgg attatgaaat gtctggttat caaaaaagca
     4141 agttagtgaa gatggacatc ttgctgaatg gagaaaaagt cgatgcatta agctttatcg
     4201 ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt tgaaaaactg aaaaaactaa
     4261 tcccacgcca acaatttgag gtgccaatcc aagcagctat tggacaaaaa atcgttgccc
     4321 gttcagacat caaagccttg cgtaaaaacg tcttggctaa atgttatggt ggagatgttt
     4381 ctcgtaaacg taaactctta gagaaacaaa aagaagggaa gaagcggatg aaacaaatcg
     4441 gctccgtcga agttccgcaa gaagccttca tggccgttct gaaaatggac gaggacgaac
     4501 cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa ataaaccatg tttacatgga
     4561 tttgccaccg gtttgccacc ggtggttttt ttcggtggca aaaatcaaga attctttcta
     4621 agattttcca tgatatcgac cgtttcattt ctcatcttat ctgttacatg cgagtaagta
     4681 tccatcgtaa ttgaaattct gctgtggccc aatcgttcag agatttcttt catttttgcg
     4741 ccattttcga gaagaagtgt agcatgagta tgtctgagag aatggaagtt aaaagagagg
     4801 gagagtgcat tcgatatcct cctagtattc catttcacta cacttggcgt aactagctca
     4861 ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga gctttccata cttcattcga
     4921 ttttctaatt gttgtttctt atgttttttc agaattgcta gcaaggtttg tccaataaaa
     4981 atcgttcgat tggaactgct tgtctttggc gtaccatata cccatgcgcc gtcattcttt
     5041 accatttgtt tctctacagt aattgttcca tttgaaaaat cgacatcatc ccacgtcaga
     5101 ccacaaactt cgccaacgcg cattcccgta taaaatccaa tattcaaagg aatatagaaa
     5161 ggatgtcctt caggagtaat ttctagcata tgatcaaagt cctcaagaga aatgattttt
     5221 agatcttttt tagtcgttgg tcgtgcttca tattttggta tttttacata cagcatagga
     5281 ttttgcttga ttaatcccca aggataaacc gccatattca gcgcattctt aaggacagag
     5341 tgagtaatag tcattgtttt cttcgagtaa ccctttttaa attcagcatt gatgaaattt
     5401 tgtaaaagag caggggagag atccgtaagt tttttctttc ctaaataacc gtttatatga
     5461 tttttgatgg taaatcggta gttttcatag gtattgtatt ttagatttag tttaacgtat
     5521 tcctccatcc aaaaatcaag gtattgtttt actcgagtat ccgtacctaa aaagtattgt
     5581 cctgtttcgt caatatctga taaaactttt cgtaaagcag cttcggcctc tggtcgggtg
     5641 tctccgccaa ctttctccac tttttttctt gagccatcat cattgatatc ttcgccgttt
     5701 gtataattaa gcgagacaaa taaaaaagcc atttatgtta cactc
//
LOCUS       DACETN010000115         5668 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DACETN010000115 DACETN010000000
VERSION     DACETN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.121"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(14..766)
                     /locus_tag="IXF98_002386"
     CDS             complement(14..766)
                     /locus_tag="IXF98_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1556673.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1130..3796)
                     /locus_tag="IXF98_002387"
     CDS             complement(1130..3796)
                     /locus_tag="IXF98_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1556674.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4137..5588
                     /gene="cls"
                     /locus_tag="IXF98_002388"
     CDS             4137..5588
                     /gene="cls"
                     /locus_tag="IXF98_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1556675.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFLLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1641 a         1192 c          990 g         1845 t
ORIGIN      
        1 taaaaagaac ccttcacgag tcgtgaaagg ttcttttttt atctgcggac caaaggagca
       61 gtctataggt cagcttagtc gtactaagga tttctcgttt ttcactgaat agtttgcgaa
      121 tgtacctggt cacaacagga tcttttgcgt acgcgtcata gtatagcttc atcaattctt
      181 cagtaattga ttcctgtgta aaataagtaa aatctttttt cttcactttg aatccttcat
      241 caattaagta tgtttcaaat tgatccatta caactggcga tgtattagga tcttcttcta
      301 tcattcctag ttgcttttcg ataggataaa aaacatcatc gattgattcc actactctga
      361 ttatcacgca ccatttgctt gactttttca tcaatttttc taattgaata agatgtacca
      421 atgctggatt tgctgctgta aaaaccattt catatgtctc atttgattgg ataaattctt
      481 ctaacgactg tttgataaag gaaacatttg gcaactcatt tttttctgct tctttttttg
      541 cattttctag cattcctgaa gaaaaatcga tcaaatcata gcgattaatt tcttttgcta
      601 aggaaagaaa tctgccagat cctccagcaa cgtctgctgc tgtttcagca ggcaggactt
      661 tctccgtttt cagataattt tttacatctt cagcaatagt tacttgcgat tcttgctgga
      721 tttcataata ttcttcagca aattcatccc attctttttc attcatttga tttcctctaa
      781 ttttttgtta tatgttttca cttttcggaa taaatcattc tcctctttta tctattctat
      841 ctcttttgat gaataataaa atgattggct ttccgtctat cataaataaa aaaagggact
      901 gtgaccaaag tcgtgtcaaa gtccctcttt ccgaataaac agtgtccaat cagatgaccc
      961 tcagtattag ctcaaaatgc tgattttaca acttcttcct taaacggtgt tctcctgcca
     1021 actcttcggt cacaagtcac aatattcaac aaacatgaga agctgtttgt tgaaattgca
     1081 ccttgtgact cagaattaaa cggcaggctc acaacctcgt tgattcgttt tatttatttc
     1141 cttgtactcg tacaatgatc aatttagtga tttccatcaa aacaattgtc acaagtgcga
     1201 ttcccataca tgtaaagact tctgtcaatc caaatgctga tgggatagag aagacttctt
     1261 tcagtcctgg gatcattgtt aagcagtaaa gcgcgctaca tacaactaca gctagtaaga
     1321 cacttctgtt agagaagaac cctgcttgga tagctgtttg tgtgtttgaa cgtgctggga
     1381 atgtttgcaa tgtccgacac caaatcaatg ttgaaaatga catggctaca ccgaattcag
     1441 gtgaatgctg catgccaagc cattgagcaa agataacagc aatcccaatc agcacaccac
     1501 gatacgttac agaaatcaat gttttgtccg taaagattcc tgaatttgga tctcttggcg
     1561 ggcggttcat cgtagcaggt tcagcttttt ctgtacctaa tgcaattgct ggcagtgaat
     1621 cattgactaa attgatgaac aacagttgta atgctgtaaa tggattggac cagtcagcaa
     1681 ctaaggcaaa gatgatcgca atgatcgcac ctaaatttcc tgcaaataga taagaaatag
     1741 ctttcttgat attatcaaac acattacgtc ctacttctac tgcgctgaca atagaagcaa
     1801 agttatcatc cgtcaatacc atcgctgctg catcttttgc cacatctgta cccgttccca
     1861 tagcaattcc gatgtctgct tgtttcaatg ctggtgcgtc atttacacca tcacctgtca
     1921 tagctgagat atgaccttta ttttgccaag cacgaacgat acgaatttta ttctcaggtg
     1981 aaacacgtgc ataaacagtg actttttcta aaacttcgtt tagttgatct tctgtcaatt
     2041 catctagttc cgtcccggtc aaagccaggt ttccttcttc aaaaatcccg atttgttttg
     2101 cgatagcaac agctgttgtt ttgtggtcac cagtaatcat gattgttttg attcctgcac
     2161 ttcttgcttc tgttaccgcc tgagtcactt cagcacgtgg aggatcgatc atagcaagca
     2221 aaccaacaag gatcaaatcg tcttcatctt caaatgataa atcaaaatta tcaagtggac
     2281 ggtaagcaaa agctagtaca cgtaatgcgc gtgttgaaaa agcttcattt tgctcttgga
     2341 attttttcat caattcatcc gtcatcggaa cgacttcacc atttagcaat acttttttac
     2401 tgcgttggaa gacgatatct ggtccgcctt taacaaacaa gtaatttttg ttatcaattg
     2461 tgtgtccagt agacattaat tttcgatctg agtcaaaagg aagttctgct tgtcttgggt
     2521 agttgtttcg gatttcctga taaggctggt ttaatttttc actaaagtcg ataaacgcta
     2581 cttccgtagg gtcgcccaat ttagtaccgt cttcactgat cgatgcatca ttcgctaaaa
     2641 cactgatttc gatcaagcga cgttcgtcag ctgtccattt tgaagggtca tttgtgaaat
     2701 ccccagtatc gccattacct aaaaagtagt caacaactgt cattttgttt tgtgtcaatg
     2761 taccagtctt atctgtacag atgatacttg tcgaaccaag tgtttcaact gctggaagct
     2821 tccgaataat ggcatgacgt tttgccattt tattcgttcc caaagataac acgattgtta
     2881 cgatagactg taatgcttct ggaatagcag ctacggctac ggctacggca aacatcactg
     2941 cgctgacgat atctgcagtc atatctcctg taccttctcc aagataaata cgaaacagtt
     3001 ggatacctaa aatcaataaa gacaatgcca aaatagccaa acttagtttt ttactgaatt
     3061 tatccagacc tcgttgtaac ggagtgactt gctcagttgt tgattccaaa aggccagcaa
     3121 cttgtccgat ctccgtatta ttcccagttg aagtcactaa gaatttaccg cgtccgtaag
     3181 ttacgatcgt tccactgaat accatatttg tacgatctcc gattggtacc gtacttgtga
     3241 tatccgttac ttctttttct gcagaagtcg actctcctgt cagcatacct tcatctactt
     3301 ttaatgatcc cgcatctagt aaacgtccat cagcaggtac ataatcgcct gcgtctaaca
     3361 acacgatatc tcctaccaca agttcgtttg ccggaatcgt tttttcctta ccatcacgaa
     3421 tcactcgagc atcgggcgct gaaaggtttt tcaacgcttc taaagaacct tctgcttttt
     3481 ttgtttggac aacactgatt atagaattca acatcaatac tgcaaaaata actaagaatt
     3541 cgatgtagtc acccatgacc atctgaatca gtgcgacaat cagcaatacg ataaccattg
     3601 catctttgaa agtttctaag aacaatttcc aagttggctt cttcggcttt tcttttagct
     3661 ggttcaagcc atctctttct aatttttcct tcgcttgcgt ggtgctcaat ccatcggttg
     3721 agctctgcgt ttgctgcatc aacgagtcga tcgtttcttt atagaccgct tgttgtgctt
     3781 gttccttgtt ttccaaattt catcccccat tttttgatct tggccctagt cctgtctgaa
     3841 aaaaaagaga cctaagacaa atacgcattt gtcttaggtc tcaccatttc atacaatacc
     3901 agaaaaccat ttggttaact tactgttgat attatcgcaa caaacgttgc cggttactcc
     3961 cctaaggaga tattcaactg tactaatatc ctaccttatt aacctacaaa aagcaactac
     4021 tttagcccga tttacctaca ttttcatgaa aggagtgaaa agtatattca ccttggtagg
     4081 cttctttctt ctatcatgct atactaatgt tattgtatgt aaggaggaac atttttgtgg
     4141 tatctagtat tataaccgcc ctttatctat taaatgcact tattgctttg gtggctattt
     4201 tgatcaaacc ccgagatgta gcagccattt gggcatggct cttagtattg tttgcccttc
     4261 ctggggtggg ctttgtatta tatttatttt tcggacgtgg attaacggat aagaaaaaat
     4321 tctatctccg acaaagtgac ttgaaagaat tagaaaactt tcagtctttt agaggagata
     4381 ccattgaaca ttacgatcct gacatgggcg ataaagacaa acagcaattt gttgacttct
     4441 tctcttcatt aaatcgtatg ccgctgacaa gaatgaattc tgtcactctt ctcacagacg
     4501 gacaagagaa attggattca ctgcttcaag atctaaaaaa agccaaacat tcgatccata
     4561 tcgaatatta cgcatttgtg acagataata tcggccagca agtcttacat gttttagaag
     4621 aaaaagccgc agaaggcgtg gaagttcgaa tattatatga tgcatttggc tctcatggca
     4681 caaaagcaaa agatttcaat cgtctaatca aaaatggtgg acatgtccat acatttgtta
     4741 cctcacaaag ggcattactt cgtttcctat tgaattacca tgatcaccga aaaatcgttg
     4801 tgatcgatgg aaagattagt tataccggtg gtttcaatat tgccaatcaa tatgtaaata
     4861 caacaaaaaa attcggctat tggcgcgata cgcatatacg gattttcggt gccgcttctt
     4921 ctttgctcca gcttcgcttc ttaacagact ggaacgtctc ggtacctgaa gaaaaaaagg
     4981 tcggctatca tttgaattat ttctttaaaa aagcagatcg agatgaatct aagcttgctg
     5041 atacatccat ccagcttgtt tcaagcggac cgaataacga aagggaacaa atcaagcttt
     5101 catttatcaa attgattact tctgctaaaa aacgtgtttg gatacagaca ccttaccttg
     5161 ttcctgatga aagtgtcatt gctgctttaa aaatcgcaac tgcctctggt gtagatgtga
     5221 aaatcatgat tcccaacaaa ccggatcatc cttttattta tcgagcaaca caatattatg
     5281 ctcggcagct gatcaaggaa aatgtacaaa tccttgtcta tgagaacggc ttcctccatg
     5341 caaaaacatt gataatggat gatgaaatct gcatggtagg ttcagcaaat caagatattc
     5401 gtagctaccg attgaatttt gaaacaagtg ctgtcattta cgatcctgag tttttagaag
     5461 aacttgctac tcagttcaaa gaagatgaga cacattgttc atccatgaca actgaaacag
     5521 tcaaggaaat gtctaactgg ctattattca agcaacaaat ttctcgatta ttttctccaa
     5581 tcctataatt tttttattca agtagagaaa aatatactaa gattaaaggg actgtgacaa
     5641 aaatcttgtc acagtccctt cttccgaa
//
LOCUS       DACETN010000116         5662 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DACETN010000116 DACETN010000000
VERSION     DACETN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.122"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(72..542)
                     /locus_tag="IXF98_002389"
     CDS             complement(72..542)
                     /locus_tag="IXF98_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1556676.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IXF98_002390"
     CDS             872..2512
                     /locus_tag="IXF98_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1556677.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IXF98_002391"
     CDS             2666..3988
                     /locus_tag="IXF98_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1556678.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4487)
                     /locus_tag="IXF98_002392"
     CDS             complement(4056..4487)
                     /locus_tag="IXF98_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556679.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4552..5388)
                     /locus_tag="IXF98_002393"
     CDS             complement(4552..5388)
                     /locus_tag="IXF98_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1556680.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1127 c         1037 g         1802 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4201 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4261 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4321 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4381 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4441 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4501 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4561 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4621 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4681 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4741 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4801 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4861 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     4921 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     4981 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5041 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5101 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5161 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5221 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5281 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5341 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5401 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5461 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5521 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5581 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5641 aagaggtcgt gagcctgccg tt
//
LOCUS       DACETN010000117         5519 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DACETN010000117 DACETN010000000
VERSION     DACETN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.123"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            132..1106
                     /locus_tag="IXF98_002394"
     CDS             132..1106
                     /locus_tag="IXF98_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1556681.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IXF98_002395"
     CDS             1088..1549
                     /locus_tag="IXF98_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1556682.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IXF98_002396"
     CDS             1599..2063
                     /locus_tag="IXF98_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1556683.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IXF98_002397"
     CDS             2077..2826
                     /locus_tag="IXF98_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1556684.1"
                     /translation="MLLKAILLCLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IXF98_002398"
     CDS             2826..3656
                     /locus_tag="IXF98_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1556685.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IXF98_002399"
     CDS             3656..4303
                     /locus_tag="IXF98_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1556686.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IXF98_002400"
     CDS             complement(4401..5177)
                     /locus_tag="IXF98_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1556687.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IXF98_002401"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IXF98_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1890 a          746 c          983 g         1900 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 tgtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tttattggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggt
//
LOCUS       DACETN010000118         5339 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DACETN010000118 DACETN010000000
VERSION     DACETN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.124"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(19..414)
                     /locus_tag="IXF98_002402"
     CDS             complement(19..414)
                     /locus_tag="IXF98_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556688.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="IXF98_002403"
     CDS             complement(404..616)
                     /locus_tag="IXF98_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556689.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="IXF98_002404"
     CDS             1688..2290
                     /locus_tag="IXF98_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556690.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="IXF98_002405"
     CDS             2645..2881
                     /locus_tag="IXF98_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ1556691.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="IXF98_002406"
     CDS             2878..3291
                     /locus_tag="IXF98_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1556692.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3810..4760
                     /locus_tag="IXF98_002407"
     CDS             3810..4760
                     /locus_tag="IXF98_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAQ1556693.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="IXF98_002408"
     CDS             complement(4757..>5339)
                     /locus_tag="IXF98_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1556694.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DACETN010000119         5228 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DACETN010000119 DACETN010000000
VERSION     DACETN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.125"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            120..1319
                     /locus_tag="IXF98_002409"
     CDS             120..1319
                     /locus_tag="IXF98_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1556695.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1346..2614)
                     /locus_tag="IXF98_002410"
     CDS             complement(1346..2614)
                     /locus_tag="IXF98_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1556696.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(2777..3423)
                     /locus_tag="IXF98_002411"
                     /pseudo
     CDS             complement(2777..3423)
                     /locus_tag="IXF98_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(3831..4334)
                     /locus_tag="IXF98_002412"
     CDS             complement(3831..4334)
                     /locus_tag="IXF98_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1556697.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(4457..5221)
                     /locus_tag="IXF98_002413"
     CDS             complement(4457..5221)
                     /locus_tag="IXF98_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1556698.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
BASE COUNT         1487 a         1141 c          891 g         1709 t
ORIGIN      
        1 attgaacaat aagaaaaaca tctttccatc ggaaagatgt ttttttgctt tttctagttt
       61 aaataacagc ttttttcatc tattttcgtt atactgtata agaaaaagga gttgaattta
      121 tgaatctaac cagagatctg atctttttag ttctctatct tattggggta ttctctatcg
      181 tttattggcg aaaacagaag aaggatcctt ccttgttctt ttacggcagt acgtggacat
      241 ttacggtaat cttaacttat tttgtcgtgt tggaaatttt ttattcgacc ttttattttt
      301 tgattccatt gttgttcttt agtatctttg ctttttctta tttcacagaa aaacgaaggc
      361 tactgaatgg tcttttattc aatgttttac tcatcagttt cggcatctac ttgtttgtct
      421 tgctgtatga aacgcaaaat atttttcttg gcggactgat cgccttgatc acgattccat
      481 tgctacttgt ccttttattt ggtatttatg gattgattgt ttttcttttt tggaacgggg
      541 tcacagtttt acgtagagaa tcgcattcat tagctaatct tttaacgttg atactagcca
      601 tttttcttac actgtttctt gtttttgact ttttcttgtt aaaatatttg ccgcaatgga
      661 taaatgcttt gtttttctgc gttcccctga tcttgatcta tctatttatc gtattctaca
      721 actttttgac tgtctcattc ttgtatcagt tcaaccggcc tcgatacaat caagacttca
      781 tcgttgtgtt aggcgctgga ttgatcaatg gagaaacagt ttctcctctt ctagccaagc
      841 gaatcaataa ggcaattgct ttttatcggg ctcaaagccg tgctacatta aatccgccca
      901 tcttattgat gtctggcggg caaggagcag acgaaaaagt accagaggcc attgcgatga
      961 aacaatatgc catggaacaa ggaatccctg aaagagacat tcttgtcgaa accaattcga
     1021 caaccacttt ggaaaacatg ctctattcaa aggaaatcat ggatcagcaa atgaaagggc
     1081 cataccgcgc gatctttagt tccaacaatt accacatttt tagagccggt ctttatgcgc
     1141 ggcaagccaa attgaaagca aatggtattg gtgcaaaaac agctttctac tacttgccta
     1201 atgcgttttt aagagaatac ctcgccatct tgatgatgaa caaaaaaaga catatgatca
     1261 tttgcggatt gcttttcgca ggctccgcat tcttatcgct gattacttta cttttctaaa
     1321 aaaacaaggt gtgacaaaac atatgtcaca ccttgttttt ttctttcatt ctcttaacta
     1381 ttggcaaaaa gagtatccct tttttgaccg ctacttgctg agcagaataa ataccgctgt
     1441 tgcctgaaca cataaagctg atcaagcaga tcataaagaa gtatactgcc gcttgtgaac
     1501 cgaacaattc gatccccatc acaaagcagg caatcggtgt atttgtcgca ccagaaaata
     1561 ctccgataaa gcccaatcct gcaagaaaag gaacagaaac atggagcaaa ggcgctagtg
     1621 ctgcaccaag cgttgcacca atttcaaaca atggtgtcac ttctccccct tgatatcccg
     1681 cgcctaaaga caaaactgtg aagaataact tcccaataaa atcatagaca tgtgcctgtc
     1741 cattaaatga ttcttgaagt aacggaagac tcaatccgag ataacgttgt gtttgcaaaa
     1801 tcaatgctgc tgccacaact acagctccac ctatcgtatt tcgacaaaca ggatgaggaa
     1861 tccattgtgc atatcgcgct tttaaaaaga cgatcgaacg gctgaatacc caaccaatca
     1921 aaccaaaagc aatcccagca acaaaaactt tgaagaacag caacatactc cattcaggaa
     1981 tcacacccat ttggtaatgt agatgagaaa caccatagct ttctgtcatg aaatttgcaa
     2041 acagtgcagc aaagaagcta gggaagatcg cttctgttcg gactttcccg attgccaaca
     2101 cttctaatcc aaataatgtt ccagctaaag gtgtgccaaa tacagaacta aaacccgcac
     2161 tgattccact gataatcaag atttcccgtt ctgatttatc caatcggaac aaacgtgccg
     2221 tattatctgc taacgctccc cccatttgga ctgctgttcc ttcacggcca acagatcccc
     2281 caaataaatg ggtagttatg gttccaaaca gtgtaagtgg aataagccgc aacgggattt
     2341 tttcttctcc accatttcct tgatcgatca ctaagttgtt tccacgacta gaaagtccgc
     2401 cgtgatacgc atagagataa gcaaaaagcg caccactaac tggtagtaga caaaggagcc
     2461 aaggatgtga tagtcggata tctgtcacaa ctgtcaggct atttaagaaa aaagcagaca
     2521 agctccccat gaatataccg agaatcgctg tgatcaccag ccacttcagc atatacacta
     2581 aaatgcgtac tggactcggt aattgttttt ccataaaaaa ttcctcctca ataaaaaaac
     2641 gcctactaaa gaaaggggct gtccccagac tttagtaggc gtcattagct atctttcgtt
     2701 agcatttaaa ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct
     2761 gtaaaaaaca ataaaattat cgttctttct ttacacgttc agcaaattca tagaaaggag
     2821 ccaatttcgt taactgttga ttacattggt cactatcatg acaaatataa ttcccctttt
     2881 tcgtatacgt gccatcagaa cctgctttcg ttgtagaaag aaaaagcgaa acattcgatg
     2941 ttttctgaca gattgcacag attcccttat ttatcgttgg tgataaagta ccgctaatcc
     3001 cttttaattt ttcatcttcg taatagacca tgaatttttt ctgcgtacct ggatcattcc
     3061 agccgatgta cgtatgttct tgcaagtcga tttcattcca agctggttgt ttgagttttt
     3121 tcacttttcg gaataatttc tcgatttgtg gtttgctagg ctgcataaac ggctgaacga
     3181 tttgcttcag ctcctccaat gctctttctg cttgcgcttt tgtcagtttt acgtctaaaa
     3241 ctgtcgttaa aaatgtatcg attctggcag ttcgtgatct aatatgtctc gaatcttttc
     3301 agctgctagt gcctgaactg tttgtaccgt ttttggatca ttgacagatg agtacgcatt
     3361 taataactgt tctacttctc ttattataaa aaaatattgg tatggtttta ttgtttgttc
     3421 catgatttta aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt
     3481 ctgcaaagta tgtgttacct gaagatcccg gactctttta aataagatac gttttgcatc
     3541 ttatcatcac cccctttaat gcttattatt ctatgtacat gattcattcg ataaataaaa
     3601 gtaaaccgta tcttgaacag atagtcaact gcaaacagaa aaaaggactg tgacaaaagt
     3661 cgtgtcaaag tcccttgttt cgaatgaatg gtgttcaaaa gctggcatct gcggtaataa
     3721 gccgaaactt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
     3781 ctgaccactt ctgtcacaac ctcaatatgg tctattattg gttttatcta ttaaatcgag
     3841 gattctttcc caaagtaacg ttgccatctc ttacgcatac ttgctgacat aggctgagcg
     3901 gcttctttga ctgcgcagta tttgtctacg aatgttacga tatatccttc tttgtactta
     3961 ggcggtgcaa tcgttgcgcc ccacatgtgc ttcaaaatga tatctcgctc aagatccgac
     4021 aattcggtga tcttttctgc attctttaca gcaattcttg gatggatata cgcatgtgtg
     4081 ccttcatcaa atttcgtcgt acgccaatca tagtagaaca gatcatgcaa aagtccagca
     4141 cgcgctgttg ctttagcatt gccaccccat ttttttgcga gcagataact atagtaagaa
     4201 acactgatgg aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt
     4261 ttcactgctt ctgtttctaa aagatcttct acataagaca tatattcttc gtcttcacgc
     4321 catttttctg tcataattat cgatctgcca cctttttgaa tttcattttc ttctgaaatt
     4381 gatctaagta taccaccagt gttcggatta ttctagtcgc cttggataaa atagccaaag
     4441 cttaatagtt ccttaatcat aaagaaaaat gataaagaac aatccctgcc gcaaccccaa
     4501 cattcaacga ttccgcattt cctttcatcg ggatatatac gttctgatca gtcaaagaca
     4561 agatttcctg acttaccccc tgaccttcgt tccccatgat tattgcgaag ttatctgatt
     4621 tttctacttt gtctaatgcg tgtgcttctt catttaattc tgtgccatac actgtaaacc
     4681 ctttgtcttt cattaaagga atcacttctg tcaaatcttt ttgcaacaca ggcaaatgat
     4741 aattgcttcc ctgcatgctg cgcagcacct ttgtactata aatatctgcc gttcctttgc
     4801 ctaataaaac accagccata tcaaatgcat ctgccgtacg gatcatcgtc cctacatttc
     4861 caggatcttg tacattatct aacaacagcc atcctcctga aaaatctggc gtgccttcag
     4921 ccgttaacgc catcactgct aagattcctt gtggtgtagg tagatccgat acagcatcca
     4981 tgacctcatc cgaaactaga aaagtagaaa tctctttgtt tgcggccagc cagtctcccc
     5041 attctttcat gcctcgctgt gtcacaaaaa gttcttttat ttctgcatca gacgacgcag
     5101 cttcttcaat cagatggaac ccttccaaaa gatattcttc cttttcttcc cgatatttct
     5161 ttttatgtag tttttttaat tctttgattc ttggattttt tgctgattgg atctctttca
     5221 cattcctc
//
LOCUS       DACETN010000120         5179 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DACETN010000120 DACETN010000000
VERSION     DACETN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.126"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            4..360
                     /locus_tag="IXF98_002414"
     CDS             4..360
                     /locus_tag="IXF98_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556699.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IXF98_002415"
     CDS             376..726
                     /locus_tag="IXF98_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ1556700.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IXF98_002416"
     CDS             755..1534
                     /locus_tag="IXF98_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ1556701.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IXF98_002417"
     CDS             1545..2222
                     /locus_tag="IXF98_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ1556702.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IXF98_002418"
     CDS             2241..3347
                     /locus_tag="IXF98_002418"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ1556703.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IXF98_002419"
     CDS             3337..4428
                     /locus_tag="IXF98_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ1556704.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..>5179
                     /locus_tag="IXF98_002420"
     CDS             4441..>5179
                     /locus_tag="IXF98_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ1556705.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1648 a          856 c         1243 g         1432 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcact
//
LOCUS       DACETN010000121         5030 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DACETN010000121 DACETN010000000
VERSION     DACETN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.127"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..182)
                     /locus_tag="IXF98_002421"
     CDS             complement(<1..182)
                     /locus_tag="IXF98_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1556706.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IXF98_002422"
     CDS             517..951
                     /locus_tag="IXF98_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1556707.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IXF98_002423"
     CDS             974..1294
                     /locus_tag="IXF98_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556708.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IXF98_002424"
     CDS             1435..1626
                     /locus_tag="IXF98_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556709.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IXF98_002425"
     CDS             complement(1697..1954)
                     /locus_tag="IXF98_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1556710.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IXF98_002426"
     CDS             2112..2375
                     /locus_tag="IXF98_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556711.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IXF98_002427"
     CDS             2399..2587
                     /locus_tag="IXF98_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556712.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IXF98_002428"
     CDS             complement(2670..2855)
                     /locus_tag="IXF98_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556713.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IXF98_002429"
     CDS             3070..3441
                     /locus_tag="IXF98_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1556714.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IXF98_002430"
     tRNA            complement(3591..3663)
                     /locus_tag="IXF98_002430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IXF98_002431"
     CDS             complement(3709..4137)
                     /locus_tag="IXF98_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556715.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IXF98_002432"
     CDS             complement(4197..4955)
                     /locus_tag="IXF98_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1556716.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1752 a          784 c          983 g         1511 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt
//
LOCUS       DACETN010000122         4958 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DACETN010000122 DACETN010000000
VERSION     DACETN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.128"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(3..1157)
                     /locus_tag="IXF98_002433"
     CDS             complement(3..1157)
                     /locus_tag="IXF98_002433"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1556717.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="IXF98_002434"
     CDS             1342..3783
                     /locus_tag="IXF98_002434"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1556718.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4113..4817)
                     /locus_tag="IXF98_002435"
     CDS             complement(4113..4817)
                     /locus_tag="IXF98_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556719.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1553 a          915 c         1039 g         1451 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgcttt tttctctatc agaaaatatg tttattatta gaatttttta tagttaaggt
     3901 taggcatctt aagaaatcag gcaactccaa gatatcttca ggtagaagag aagtcgccta
     3961 acattgtagg atcataggtg atttgttgga caggtcaagc tgatatttga gcctgatgtt
     4021 agaaggaatt tctttttact tgctgtagaa attattatat gactaaaaag gctgggattt
     4081 taaatcccag ccttttatga caaatagaat ttttatcgat cagtaatcgc tgtcgctgca
     4141 aattgttcga tcaaaggaat caaagtcaat ttgactttgt atttccgttc gagtgctttt
     4201 tcgataacga actcattttc agtagtcata gaaatagttg ctaatttacg aggttcatca
     4261 gcattttttt cattaataac tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac
     4321 tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt
     4381 gtattttcag ttgtgtccgc ttttggtttt tccgtttttt cttctggcac actgactgtt
     4441 tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt catcgttttt caatcgttga
     4501 tcattccagt aagacacgat ttcaataaga agattgtcac ggaatcgttc gaaaatcccg
     4561 ttttctcctg aacgaattct ctttaataca gtattgaagt tgtcatcgat tggcacgttg
     4621 atccgtgtat cttcatttgt tttcccagct aatccgatcg ttaagatttg acggtttttg
     4681 ggatcaagca tgacttgtaa cggcaaacca taacgtcgca agctctcttc ttctagagaa
     4741 cggaataatt gtcctcttac accatccaat gaaggacgga attgatttaa taatttttgt
     4801 ttctgttttt tcttcatatg ttctctcctt cgctcatacc ctttcgatta taagaaaaaa
     4861 acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa
     4921 aatttgcgta ttttcccgtt ttttgaaaga aaaataaa
//
LOCUS       DACETN010000123         4927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DACETN010000123 DACETN010000000
VERSION     DACETN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.129"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            83..970
                     /locus_tag="IXF98_002436"
     CDS             83..970
                     /locus_tag="IXF98_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1556720.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IXF98_002437"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IXF98_002437"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1556721.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IXF98_002438"
     CDS             2272..2718
                     /locus_tag="IXF98_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1556722.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IXF98_002439"
     CDS             2991..4343
                     /locus_tag="IXF98_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1556723.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IXF98_002440"
     CDS             4514..4801
                     /locus_tag="IXF98_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556724.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1391 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 ccccttt
//
LOCUS       DACETN010000124         4807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DACETN010000124 DACETN010000000
VERSION     DACETN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.130"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXF98_002441"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXF98_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1556725.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IXF98_002442"
     CDS             complement(3250..3768)
                     /locus_tag="IXF98_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1556726.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACETN010000125         4726 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DACETN010000125 DACETN010000000
VERSION     DACETN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.131"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IXF98_002443"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IXF98_002443"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1556727.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IXF98_002444"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IXF98_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1556728.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IXF98_002445"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IXF98_002445"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1556729.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACETN010000126         4512 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DACETN010000126 DACETN010000000
VERSION     DACETN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.132"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..798
                     /gene="prmA"
                     /locus_tag="IXF98_002446"
     CDS             <1..798
                     /gene="prmA"
                     /locus_tag="IXF98_002446"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1556730.1"
                     /translation="DKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLA
                     IGKNKVTVSEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGM
                     AFGTGTHPTTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVA
                     VRSAKENMDMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQT
                     GTLIVSGIIEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            799..1563
                     /locus_tag="IXF98_002447"
     CDS             799..1563
                     /locus_tag="IXF98_002447"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1556731.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            1796..4009
                     /locus_tag="IXF98_002448"
     CDS             1796..4009
                     /locus_tag="IXF98_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1556732.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            4020..4466
                     /locus_tag="IXF98_002449"
     CDS             4020..4466
                     /locus_tag="IXF98_002449"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1556733.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
BASE COUNT         1584 a          793 c         1003 g         1132 t
ORIGIN      
        1 gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta ttttccggaa
       61 acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca attacctgag
      121 tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga aagcaactgg
      181 gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg
      241 ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac actagatcct
      301 ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac agcactagaa
      361 acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt
      421 attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc
      481 gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt ccatgtatca
      541 gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc gaacatttta
      601 gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca aacaggcacg
      661 ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc catgttagaa
      721 caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat tattttgaaa
      781 aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga atcaaaagac
      841 tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag gatggaaccc
      901 gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga aatcacggag
      961 attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca aaaggaactg
     1021 ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga ttggatcgtt
     1081 cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc agcctctgtc
     1141 gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa
     1201 gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt ggaaacgaaa
     1261 caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta tgaggaatca
     1321 gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa accagatagt
     1381 cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt cgattatttt
     1441 gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac agaaacagca
     1501 cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc ttctttcagc
     1561 taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata gtcaacaatt
     1621 tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa aaagctttta
     1681 aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac aattatcaaa
     1741 cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac
     1801 aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat acatgggtcc
     1861 cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac atgacggaca
     1921 gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag gtatattagc
     1981 agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg ttgttgaaga
     2041 tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg ctatgttagt
     2101 ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc agctagcaga
     2161 aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca tggtcaaatt
     2221 agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata agcaaagaag
     2281 gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag ggatcagccg
     2341 gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac agtattaccg
     2401 tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt
     2461 tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct atggacggcc
     2521 aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat
     2581 ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt atgctgtatt
     2641 aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt atattgccat
     2701 gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg caggaaatcc
     2761 ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg gggttgctgc
     2821 acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca
     2881 attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg cttctgaatt
     2941 tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca caccaaaagg
     3001 agacgttact gagttaccaa aaggatccgg accattggac tttgcataca gtatccatac
     3061 agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta
     3121 caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat ttggcccaag
     3181 tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca aacgtttctt
     3241 caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat
     3301 tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc aagaagcact
     3361 cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt atggagaagt
     3421 tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga
     3481 gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat
     3541 gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac taattcgtat
     3601 cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca ctaaaggcag
     3661 agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac caaatgtagc
     3721 agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag agtatgatgc
     3781 agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac ttcaaacagt
     3841 caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag ataaaatggc
     3901 gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta tcgtggataa
     3961 aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg aggacagata
     4021 tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg
     4081 gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat acgcaaaaag
     4141 atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa
     4201 aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct caattcacac
     4261 tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca cgaccagaaa
     4321 cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc atcacagtcg
     4381 ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga cctgtaacga
     4441 tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa aactatgaaa
     4501 cacgtcgctt aa
//
LOCUS       DACETN010000127         4488 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DACETN010000127 DACETN010000000
VERSION     DACETN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.133"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            93..422
                     /locus_tag="IXF98_002450"
     CDS             93..422
                     /locus_tag="IXF98_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1556734.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IXF98_002451"
     CDS             412..699
                     /locus_tag="IXF98_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1556735.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYDQLKKLI"
     gene            985..1842
                     /locus_tag="IXF98_002452"
     CDS             985..1842
                     /locus_tag="IXF98_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1556736.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1843..2391
                     /locus_tag="IXF98_002453"
     CDS             1843..2391
                     /locus_tag="IXF98_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556737.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2651..3379
                     /locus_tag="IXF98_002454"
     CDS             2651..3379
                     /locus_tag="IXF98_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1556738.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3409..>4488
                     /locus_tag="IXF98_002455"
     CDS             3409..>4488
                     /locus_tag="IXF98_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ1556739.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEVIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1629 a          598 c          921 g         1340 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgatca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
      781 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
      841 atttcagctt ttccttattt atgtactacc ttttccttat taattataag gtagtaccta
      901 tatatctttt ttttggtagt aactaaaaat aaatatgtta taatagtatc aaatgaatat
      961 tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat atgtaatcgg taattttaaa
     1021 ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa
     1081 ggaaaaagaa ccttgataat tgacttggat atgcaaggaa atacttctga tgtgatgaac
     1141 ttaacgcata tgaacttcac taacgaagaa ggaggcggag aaggtgaacc gattgtttac
     1201 gaaaatactg tgactgacgt gttgataaac aatcttccac ctaaagaggc tgtctataaa
     1261 gtaatcaata acctctatat acttccagca gacatgtcct ttgaaatgta tgatgattgg
     1321 atcaaagacc aatataatga ttcattatcc caattcaaat atatgaaaga aaagctagaa
     1381 ccattatttg atgagtttga cgtcatctat ttagatgtcc caccttctat ttctgtatat
     1441 agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg tcttacagac ccaagtaaaa
     1501 tcgatgagaa atgcgatgca atatcttgag tatatggact tttttgtgaa agaatttgat
     1561 acaaatttat atgttgctgg tgttattcct ttcatgttgg aaagcggtga cgcagtagat
     1621 caggaaatgt acaaacaagc ccaagagatt tatggggaac atttgattaa aaatgttgta
     1681 ctcaaaaatg cacgcttaaa acgatacgac ggatcaggta ttacgatgga gaagacaaag
     1741 aaaggtctct taaaacaatg ggatcgcaaa agccacgaat tatttatcag tattttgaaa
     1801 gaattggaag aacacgagaa ttggtatgcg gaggtggagt aattggctga catcaaagga
     1861 cttgatattt ttgggaacaa caaaggactc gccaaaataa aaaaagaagc agaagcaaag
     1921 aaaaagcagc aagcaattac accatctgaa aataagcaat tagaagtgca ccaagttcaa
     1981 gaaataaaaa cttcctccaa tgttgaaaga cagagtagag gaagaacaaa aaaagttggc
     2041 gcaccaatca gaaagtttga taaggtttat tccaccaagc atcctttaaa actgtcgact
     2101 ttgttaaatg cgacttccag agttttagta gaaaagtatg agactaatat gacaagagat
     2161 gaattgctta gaaaagcttt agatgagtat attaaacaga atcttacgca agaagataag
     2221 caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt tcagagaaaa atatccaatt
     2281 gttccagaat tagatgatcg tggagaaata ataagaacta ccagtcagat aaaacaagat
     2341 accgaagact cactgcgcaa agggtgggga atgaataata aagttagata ataatcaaag
     2401 aagaactaag taactacctt taaacggtag ttacttagtt cttctttgat ttgcgtgaaa
     2461 tagaggggat ttatttttct ggttacgtga ttaaaaaatc caaaaaatga gcataggaac
     2521 ggttatgatt ttagggtgtg ttttaatgag atattgtgat ttcattaaca agtgtataat
     2581 aatctgttgt tgagttagta aataagtaat agagctaact ctaaaataaa actattagga
     2641 ggagatgtag gtgaaaaagt ttaaaaagaa aatatattta ggattggttg ttggggtgag
     2701 tatgtttggg ttcatggctc atactaaaca agtaactgca gatactaacc aaaaagaaag
     2761 gattatgagc caattagatg actctcaaat agagtatgta cccaatgagc taattgtcag
     2821 tttaaaggct acacaaagaa tttcaacaga gaaaaataaa ccgcaaaatt tattgggtgt
     2881 aaaagttatg gtggaaaaag atttgactct taggacaaat gaaagcgatt ataataagcg
     2941 agatcaagta ttattagtga aattgaacga agaaattagt gtggaagagg cagtaagaac
     3001 atttaatgct tctgatatgg tcaattatgc agagccaaac tatgtggttg aactagagcc
     3061 gttgaatgat gttgaggagc caaatacaac tgattcttta ggaaatcgat caattattct
     3121 taacgatcct aattggagta aacaagctaa tttaagagag attagcatgc catccgcttg
     3181 ggaagaaacc aagggttcta aggatgtgaa ggtagcggtc attgattcag gaattgatta
     3241 tcaacatgaa gatctttctg gaaatgtaga tgaaagtttg agatacgatt tttttgacga
     3301 tgatacagat cctatggata ctatgggaca tggacacata ttgccgggat tattggtgct
     3361 caaacaaaca acgaattagg tgttgctgga ataaattggg atgttacgat ggtacctata
     3421 cgtgtatttg gtagtagtgg aggtaccagt ttggcaatct taactgaagc tattaattat
     3481 gcatcccaaa aagggtttcc aattatcaat tttagtatag gtagtgttgt ttatagcagt
     3541 acagaagaag taattaaaaa ctatcctgga ttattcgtag cagcagctgg aaataatggg
     3601 atagatatga ctgataaacc tttttaccca gcttctttgg atatacctaa tattattact
     3661 gtaggaaatt ctagtggtta tgaacgaagt tctgattcaa attatagtaa aacaggtgtt
     3721 gatttattcg ctagagggtt tcgtgttttt agtacatttc taggaagtaa atacacgtat
     3781 atgtcaggta cctcgatggc aacacctcat gtcactggta gtgctgcatt agcgttgtca
     3841 ctagatcctg atctatctac tgatgaatta aaaaaaatca ttttaaatag tgttgattca
     3901 ttttatgaac tatctgattt atgtcttaca gggggaagac taaatacgaa taatgtcgta
     3961 cgacaagtaa cgggtaaact tactgcatct aatatcacat taagattaaa tagtgaatgg
     4021 tcagatgaaa ttgctaaaga gatgtgtcac gttcactgga ttgaccagtc aggaaatgat
     4081 ttgacggatc aagtggttgt tctaaaaaac gaagtggata catctaactt tggcgattac
     4141 gaagtagtat ttgcatcacc tgatttaaca gatcaaatct cagttacagt gaccgtacct
     4201 cgattacgta aacttacgct aggaaaagaa acagcaacca ttgatattgg ttccgcatgg
     4261 gatagtgtca tagccattga tacttttcaa gtgaaagcta tagatgattt tgggaatgat
     4321 gttacaaatc aagtacaggt tactattaat gctgatatag atacaattaa ttcaattcaa
     4381 gcagtgatta agtttgaagt tccagaatta ggcttagccc aaattggtag actgaatatt
     4441 actgcaaaaa atagtacaat caatggattg aatgaagtta gcatcgag
//
LOCUS       DACETN010000128         4374 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DACETN010000128 DACETN010000000
VERSION     DACETN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.134"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(83..1051)
                     /locus_tag="IXF98_002456"
     CDS             complement(83..1051)
                     /locus_tag="IXF98_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1556740.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IXF98_002457"
     CDS             complement(1498..2904)
                     /locus_tag="IXF98_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1556741.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IXF98_002458"
     CDS             complement(3126..3488)
                     /locus_tag="IXF98_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556742.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IXF98_002459"
     CDS             complement(3492..3800)
                     /locus_tag="IXF98_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556743.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IXF98_002460"
     CDS             complement(3809..4228)
                     /locus_tag="IXF98_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556744.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IXF98_002461"
     CDS             complement(4221..4361)
                     /locus_tag="IXF98_002461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556745.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACETN010000129         4324 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DACETN010000129 DACETN010000000
VERSION     DACETN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.135"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1..657)
                     /locus_tag="IXF98_002462"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="IXF98_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(662..1429)
                     /locus_tag="IXF98_002463"
     CDS             complement(662..1429)
                     /locus_tag="IXF98_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1556746.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1918..2343
                     /gene="lacA"
                     /locus_tag="IXF98_002464"
     CDS             1918..2343
                     /gene="lacA"
                     /locus_tag="IXF98_002464"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1556747.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2360..2875
                     /gene="lacB"
                     /locus_tag="IXF98_002465"
     CDS             2360..2875
                     /gene="lacB"
                     /locus_tag="IXF98_002465"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1556748.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2886..3818
                     /gene="lacC"
                     /locus_tag="IXF98_002466"
     CDS             2886..3818
                     /gene="lacC"
                     /locus_tag="IXF98_002466"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1556749.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3821..>4324
                     /locus_tag="IXF98_002467"
     CDS             3821..>4324
                     /locus_tag="IXF98_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HAQ1556750.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYD"
BASE COUNT         1431 a          681 c          845 g         1367 t
ORIGIN      
        1 gcttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg
     1801 tttgaatgct tgatattata taaacaaaga aatgatgaaa acgttttttc gaacattttg
     1861 caaaatgttt tctacttcta cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg
     1921 gctattgttg ttggtgcaga tctcaaaggt acgagattaa aagatgttgt gaaaaatttt
     1981 cttgtagaag aaggttttga ggtaattgat gtgactaagg acggacaaga ttttgttgat
     2041 gttaccttgg cagttgcctc tgaagtaaat aaagatgagc aaaacttagg tattgtaatt
     2101 gatgcttatg gagctggtcc attcatggta gcaactaaaa ttaaaggtat ggttgcagca
     2161 gaagtttcag atgaacgctc agcttatatg acacgtggac ataacaattc acgaatgatt
     2221 acggttggcg ctgaaatcgt cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc
     2281 aatggtaaat atgatggcgg acgtcatcaa atccgagtag atatgcttaa caaaatgtgt
     2341 taaggaaagg agttaaggaa tgagaatcgc aattggatgt gaccacattg tcacagatga
     2401 aaaaatggca gtatcagaat tcttgaaatc aaaaggacat gaggtccttg atttcgggac
     2461 atatgaccac gcacgtactc actaccctat ctatggtaaa aaagtcggtg aagcagtagt
     2521 aagtggtcaa gcagacttgg gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc
     2581 agtcaataaa gttccaggtg ttcgctcagc gttggttcgt gatatgacat cagctatata
     2641 tgcaaaagaa gagttaaatg cgaatgttat cggtttcggt ggaaagatta ctggtggtct
     2701 tcttatgaat gacatcattg aagctttcat tgaagctgag tacaaaccaa cagaagaaaa
     2761 taaaaaattg attgcaaaaa tcgaacacgt tgaaacacat aatgcacatc aagcagatga
     2821 agatttcttt acagaattcc ttgaaagatg ggaccgtggt gactaccacg attaaggtat
     2881 aacaaatgat tctgacagtc acactaaatc cttcagtaga catctcttat cctctagaaa
     2941 cattgaaaat tgatacagtg aatagggtga aagatgttag taagacagct ggtggaaaag
     3001 gcttaaatgt tactagagtc ttgtatgaat ctggtgataa agttactgct acaggtttct
     3061 taggtggtaa aattggtgaa tttattgaaa gcgaattaga acaatctcct gttagtccag
     3121 ctttttataa aatctcagga aacactagaa actgtattgc tatcttacac gagggtaacc
     3181 aaacagaaat tttggaacaa ggaccaacag tttctcatga agaagcagaa ggttttcttg
     3241 accactatag caatcttatt aaacaatcag aagttgtgac aatttcagga agtttacctt
     3301 caggacttcc caacgattat tatgaaaaac ttatccaact tgcttcggat gaaggagtag
     3361 cagtcgtttt ggattgttca ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac
     3421 ctactgccat caaacctaat aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa
     3481 aagatattga agaactcaaa gacgttctta aagagtcact ttttagtggt atcgaatgga
     3541 tagtggtctc attaggtcga aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg
     3601 tagatattcc tgatatccca gttgttaatc cagtcggatc aggggactca acggtagctg
     3661 gtattgcatc agctttaaat agtaaaaaaa gtgacgctga cttattaaaa catgcgatga
     3721 cattgggtat gttaaatgct caagaaacaa tgacagggca tgtgaatatg actaattacg
     3781 aaacactaaa ctcacaaatt ggagtaaaag aggtataaaa atggtactta cagaacagaa
     3841 acgcaaatct ttggaaaaaa tttcagataa aaacggtgtt atctcagctt tggcatttga
     3901 ccaacgtggt gctttgaaac gtttgatggc acagtatcaa gatacagaac caactgtagc
     3961 tcaaatggaa gaactaaagg ttttggttgc tgatgaactt acaaaatacg cttcttcaat
     4021 gttgcttgac cctgaatatg gacttccagc aacaaaagca ttggataaag aagcaggtct
     4081 tcttcttgcc tatgaaaaaa caggttatga tacatcaagt acaaaacgtt tgcctgactg
     4141 cttggatgtt tggtctgcaa aacgtatcaa agagcaaggc gctgatgccg ttaagttctt
     4201 gctttactat gatgtagaca gctcagatga acttaaccaa caaaaacaag cttatattga
     4261 acgtgttggt tctgaatgtg tcgctgaaga tattcctttc ttcttggaaa tccttgctta
     4321 tgat
//
LOCUS       DACETN010000130         4253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DACETN010000130 DACETN010000000
VERSION     DACETN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.136"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(207..1169)
                     /locus_tag="IXF98_002468"
     CDS             complement(207..1169)
                     /locus_tag="IXF98_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1556751.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IXF98_002469"
     CDS             complement(1171..2610)
                     /locus_tag="IXF98_002469"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1556752.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..>4253
                     /locus_tag="IXF98_002470"
     CDS             2795..>4253
                     /locus_tag="IXF98_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HAQ1556753.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDP"
BASE COUNT         1331 a          741 c          693 g         1488 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caa
//
LOCUS       DACETN010000131         4226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DACETN010000131 DACETN010000000
VERSION     DACETN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.137"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..345
                     /locus_tag="IXF98_002471"
     CDS             <1..345
                     /locus_tag="IXF98_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556754.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="IXF98_002472"
     CDS             426..1046
                     /locus_tag="IXF98_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556755.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1376..2602)
                     /locus_tag="IXF98_002473"
     CDS             complement(1376..2602)
                     /locus_tag="IXF98_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1556756.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2674..2832)
                     /locus_tag="IXF98_002474"
     CDS             complement(2674..2832)
                     /locus_tag="IXF98_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1556757.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3520..>4226
                     /locus_tag="IXF98_002475"
     CDS             3520..>4226
                     /locus_tag="IXF98_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556758.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIG"
BASE COUNT         1449 a          591 c          691 g         1495 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagctgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agaaattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaaatt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtctcaa aaaggctcgt
     1261 aaagcatctc aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt
     1321 tcaattgttt gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat
     1381 cacatttatt caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg
     1441 tatttacgta tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat
     1501 tcatatttgc tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag
     1561 ataaatgagc caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat
     1621 cagcgtgaac agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat
     1681 ctggtgtttg attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc
     1741 ttagtaatgc cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa
     1801 atttcttttt ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt
     1861 cgatatattt ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa
     1921 gcatgaaaag agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg
     1981 cttctatcca agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc
     2041 tttcttctaa agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt
     2101 taaattcaat ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga
     2161 taatttttat atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag
     2221 tatttgaatt tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga
     2281 atatatcttt agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct
     2341 gacctagttc atatctagga agccattttg attcatagaa ttccttaaat tttatttttc
     2401 cttttaattc tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca
     2461 ttgcattttc ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg
     2521 atgtaattcc agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc
     2581 catttgaaag ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc
     2641 gctattctta gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa
     2701 atctttgata tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt
     2761 atatatacga atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg
     2821 ttttttattc aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt
     2881 ttgaattgct ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt
     2941 acggaagtat cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga
     3001 aatctctaag tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag
     3061 cagggatcgt tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct
     3121 attcactatt aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg
     3181 agtttactga ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa
     3241 tgttttgatg cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata
     3301 cgccaataaa gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt
     3361 ttgaaagcta cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa
     3421 cagaaaaaaa tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat
     3481 atgatcgtga taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt
     3541 aatgtttgtt ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt
     3601 gctttaacag gtattattgg tccaaatggt gctggaaaat ctacattgct taaagctatg
     3661 ttaaatatta tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa
     3721 ctggtgaaga ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa
     3781 gtaaaagaat gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa
     3841 aatgcagaat ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct
     3901 aatcatcaaa taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt
     3961 cttgctcaaa atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt
     4021 agtgaacgaa taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta
     4081 attgtgcatc atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat
     4141 cgaaatctaa ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa
     4201 gcctatggag atactatatt tattgg
//
LOCUS       DACETN010000132         4216 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DACETN010000132 DACETN010000000
VERSION     DACETN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.138"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            624..3263
                     /locus_tag="IXF98_002476"
     CDS             624..3263
                     /locus_tag="IXF98_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1556759.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IXF98_002477"
     CDS             complement(3431..4129)
                     /locus_tag="IXF98_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1556760.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1277 a          805 c          912 g         1222 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgatgagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttatt
//
LOCUS       DACETN010000133         4116 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DACETN010000133 DACETN010000000
VERSION     DACETN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.139"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..85)
                     /locus_tag="IXF98_002478"
     CDS             complement(<1..85)
                     /locus_tag="IXF98_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556761.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            161..316
                     /locus_tag="IXF98_002479"
                     /pseudo
     CDS             161..316
                     /locus_tag="IXF98_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            335..1612
                     /locus_tag="IXF98_002480"
     CDS             335..1612
                     /locus_tag="IXF98_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556762.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="IXF98_002481"
     CDS             1622..2278
                     /locus_tag="IXF98_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1556763.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="IXF98_002482"
     CDS             2278..3654
                     /locus_tag="IXF98_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1556764.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="IXF98_002483"
     CDS             3784..>4116
                     /locus_tag="IXF98_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1556765.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACETN010000134         4073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DACETN010000134 DACETN010000000
VERSION     DACETN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.140"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            591..1280
                     /locus_tag="IXF98_002484"
     CDS             591..1280
                     /locus_tag="IXF98_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1556766.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IXF98_002485"
     CDS             1267..2475
                     /locus_tag="IXF98_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1556767.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IXF98_002486"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IXF98_002486"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1556768.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          702 c          812 g         1239 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggttgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DACETN010000135         4015 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DACETN010000135 DACETN010000000
VERSION     DACETN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.141"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(176..1462)
                     /locus_tag="IXF98_002487"
     CDS             complement(176..1462)
                     /locus_tag="IXF98_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1556769.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IXF98_002488"
     CDS             complement(1455..2723)
                     /locus_tag="IXF98_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1556770.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IXF98_002489"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IXF98_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1556771.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IXF98_002490"
     CDS             complement(3495..3941)
                     /locus_tag="IXF98_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556772.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1137 a          816 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaat
//
LOCUS       DACETN010000136         3993 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DACETN010000136 DACETN010000000
VERSION     DACETN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.142"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(113..388)
                     /locus_tag="IXF98_002491"
     CDS             complement(113..388)
                     /locus_tag="IXF98_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1556773.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(360..1313)
                     /locus_tag="IXF98_002492"
     CDS             complement(360..1313)
                     /locus_tag="IXF98_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1556774.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1925..3418
                     /locus_tag="IXF98_002493"
     CDS             1925..3418
                     /locus_tag="IXF98_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1556775.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3553..3849
                     /locus_tag="IXF98_002494"
     CDS             3553..3849
                     /locus_tag="IXF98_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1556776.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3873..>3993)
                     /locus_tag="IXF98_002495"
     CDS             complement(3873..>3993)
                     /locus_tag="IXF98_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556777.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1328 a          653 c          717 g         1295 t
ORIGIN      
        1 atgttgtata cccccaaaca atacacatat tttgaaggat taggattaat aaagctaaaa
       61 atatagtaag tatactaaat atatttacta tatctagtat acttactata ttttaatctt
      121 ttgatcgtct attatatata atccgttcct cgtcgtttaa agtatcaaca taggcgtcca
      181 ttgcaagttt gactatttca tacataggaa tatcttttgc ataactaaga tcccgaatgg
      241 tttttagtac gggaggatct acttgaactg gttttctttc tttcgcagtg tactttcgtt
      301 cccctaactc gacattaata tcatctttaa ttgaaatatc ttcagcacca attttcttct
      361 tatctttgtt cgttggtaat aatgccattt agtttaatct cctttccaag aggtaggata
      421 tgattggcca ttgagtcagc atatttaggt gtataatcaa atccttcaat atcaccatgt
      481 tgttcatagt attcaattct ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac
      541 catcttcgat ctacataatc atttaattgg ataccattct caccatacat ttctaaccga
      601 tccgagcctt taacaatgct tttaaaaatg tttgattcac catacttatc aattgttttt
      661 tgatagtttg cgtgttgaga ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg
      721 atttgaagat gagactcttc aaaattcgtg acaatcggaa ctaaaacttt attaataaaa
      781 tcttctgttc cttccattgc gtaccgtttt aattcttgca ttgcaataat ataatctgca
      841 gcagttaaga atgcacgaac cacattatct gttgatggtg ggcagtcgat aaagatataa
      901 tcgaaatttt ttctcaattc ttctaaaggt ttaatgaagg ccaatgatct cttacgacga
      961 tcaggatatt tttcggacaa gtattcatta agttcttcag atccaggaga gccagccata
     1021 atatataaat gtggacttac ttgagtaata cctgaaaccc agtccccttt tttgattgct
     1081 gcagacatgg atcgatcaaa gctagtaatt tgatacgttg atccaatgtt ggaagtagta
     1141 tttctttgtt ggtcataatc aattagcaac acgtcttttt caaaaagttg actagaaaca
     1201 atcgctaata aatcattgtc tgtagtctta ccaatgccgc cttttctacc tgtaagcatt
     1261 acaacttttc ctagtttatc tgatgtttta aatgcagtcc gtaagttttc catagttccc
     1321 tccaatttcc tatactaaat atatttagta tacttcatat acttaatata ttcgatatat
     1381 ctaatatact ccaaattgat aagaattcag actttataag gaatatagtg gatatactaa
     1441 atatatttgc tatattcagt atatcagata tattcaaaat gtcaaaatat atttgtgcgt
     1501 tttgcgttga caatttagag tgaaaagagg taaacttaat tctatagaaa ccacagataa
     1561 ttcactattt acaaagtagc aaaagcagtg gtactcttta aacagaaaaa ttacataaaa
     1621 aaaagagacg gaaataattg attttagcag aatcaattat ttctgaatca catggttagg
     1681 acatgtgttc acgctcgtct caagaatatg taagtagcta ccaactactt atattaaaag
     1741 ttgatcggat accttttccg attgccttta ttctaccaaa aaaaggcgag caactcaaca
     1801 gaagaacgtt tatttagtgt acttaaatta aatattctta aacactgaag agagaacaca
     1861 tactatggct gagattaaag ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca
     1921 atcgatgttg agcgaaattt attcagctat tcttaaaaac ggcttacgta catacaaata
     1981 tcgaaacagc accatgaaac ctgcaggtca tattgattcc agtaggagtg gtgctatctt
     2041 tgcgtaccgt agcaagcaat taatgaatgt tgggcgtggt atggtcatta cttcggaaga
     2101 agctatttta gagaacgaaa agaaactaac gcattggaca cctaatacct atcgctttgg
     2161 tacttatgcg gatgattatc gaacagtggt taaaggtcat tctgagaaaa acttaagtca
     2221 aatcaacaca tttgtagttg atatcgattc aaaagagaac catcaaggcg aaattatttt
     2281 agcctgttta gatcaagtgg gctacatgcc aacgctaatt ttagaatcag atcatggtta
     2341 tcaagtttat tttgtactaa agacaccagg ctatgtcaca aagaaaagca atttcaaggt
     2401 gatcgatgta gcaaaaaaaa tttcaaaaac aattcgtcta cagttggctg aaaaattgtc
     2461 cggagtggat ctaggatgta accacttcgg aattgctcga ttcccacaag aaaataatat
     2521 tgtttttaaa gaaatgaact atcagtattc ttttgctgac tggattcaat ggtcgatgaa
     2581 gcttgaatca gatcaaaaaa caaaagaaga tcgagaacga aaactaattg tctttcctga
     2641 gaaaaaagaa attcgtcaag tagatgaacc gtggttcgat atgttacttc ataaagcaaa
     2701 catcgtcggc ggagaaggac gtcttggtcg aaataatgtg attttcacgc tttctttagc
     2761 ttattactct tcaggttatg cattggatac ttgcgaatac aatatgttca cattcaacga
     2821 acggttacag gaaccgctat ctgaagcaga attagtaaag attgtacgta gtgcttattc
     2881 tggtaattat caagcagcaa gcagagaatt cattattgaa ttgtgccagc aatgggtttc
     2941 accaacctta aaaaataatg atctgttcat tcaacgacgt ggctggtgga aatttaaaaa
     3001 gccacgagat caaagaaaat atagtcataa aaatgaatgg aaagaagatc tattggctta
     3061 tctaagcgaa aaaagtcttg ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc
     3121 ttcacaacta acgatgccca agcgaacact tgataagatc ttaaatgagt tacgagagga
     3181 gaataaagtc ttctacaagg taacacgagg aagaaatggc ggaatgatca ttgcttcagt
     3241 caaaacgttg ttcgcgaggg ttattcaact aagtaaagaa aagagactag attattcaac
     3301 aaggatcaca gaggtatttg gactcaacga gagcttagta gaagagacat ttagacaatt
     3361 atcaaccccc ttgaaccctc ttaaagaagt gaatcttttt gaagtagata caggttgaaa
     3421 aataaaaggt gcaaattgcc ttatacaata tatctatgat acttctgtag gtgttgctcc
     3481 ctttgcctag ctacgtgtag gtggtatact tataactgcg tatgcagtct tatgtatcat
     3541 ggaggaagtt gtatgtcgtt aaaaaattat gtctattcac atcctgttaa tgtgtttatc
     3601 attggtcgtt tgggattatc tgtggaagtg ttctgtgagc tttatggttt taagcagggg
     3661 actttgtctt cttgggttac cagggaaaaa acagttgctt ctttgccgat tgagtttctt
     3721 catgggttgt ccttagcttc tggggagact atggatcagg tatatgattg tttgtgtgtg
     3781 ttagagcagg agtatattga gtataaaata gctaacgagt taagaaaaag gaagaagtat
     3841 atccagtagt agtttttatt tactcttaat tgtcaaatta agttagacaa atcatcaata
     3901 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat tcagtgattt tctaggaata
     3961 ttatttcgtt tcgatgcgac agattgaata aaa
//
LOCUS       DACETN010000137         3925 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DACETN010000137 DACETN010000000
VERSION     DACETN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.143"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(573..1130)
                     /locus_tag="IXF98_002496"
     CDS             complement(573..1130)
                     /locus_tag="IXF98_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556778.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IXF98_002497"
     CDS             complement(1309..1920)
                     /locus_tag="IXF98_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556779.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IXF98_002498"
     CDS             complement(2033..2290)
                     /locus_tag="IXF98_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556780.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IXF98_002499"
     CDS             complement(2887..3165)
                     /locus_tag="IXF98_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556781.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IXF98_002500"
     CDS             complement(3199..3450)
                     /locus_tag="IXF98_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556782.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACETN010000138         3626 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DACETN010000138 DACETN010000000
VERSION     DACETN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.144"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(148..969)
                     /locus_tag="IXF98_002501"
     CDS             complement(148..969)
                     /locus_tag="IXF98_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1556783.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1066..1344)
                     /locus_tag="IXF98_002502"
     CDS             complement(1066..1344)
                     /locus_tag="IXF98_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556784.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1612..3621
                     /gene="metG"
                     /locus_tag="IXF98_002503"
     CDS             1612..3621
                     /gene="metG"
                     /locus_tag="IXF98_002503"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1556785.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         1203 a          667 c          712 g         1044 t
ORIGIN      
        1 ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac gaatacatca
       61 aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt tcttgaatga
      121 ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga gctgatagat
      181 ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat attggagcat
      241 agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga atttagcaaa
      301 tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa cttgcggctc
      361 gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac tatggtcaaa
      421 agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga tagccacttc
      481 tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc gtacttcctt
      541 ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc tgactgtgac
      601 gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct caatattttc
      661 ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt ctccagttga
      721 ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag gatgcccttt
      781 tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca tcagtacgat
      841 atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat accagttaag
      901 aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt tagaagcaaa
      961 acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat tgtagcagac
     1021 tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa tatgaaaaat
     1081 aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata ctttataccg
     1141 ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga taaaacgcaa
     1201 aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat aaccatacat
     1261 acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa tactaagtgt
     1321 aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata gatagtcttt
     1381 attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat gaaacaaaac
     1441 aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa cgtagataga
     1501 agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt tttttatggt
     1561 atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc cgtggcagaa
     1621 aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact tcatatcggc
     1681 aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt gatgggcttt
     1741 gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac aaaagcagaa
     1801 gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt caagaaatta
     1861 tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga ttaccataaa
     1921 gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta cttaggtgaa
     1981 tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca acttgcagaa
     2041 gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg acacgaagta
     2101 gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga ccgtctattg
     2161 gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa cgaaatgatc
     2221 aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac ttttacttgg
     2281 ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga tgcattatcc
     2341 aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga aaaatactgg
     2401 cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat ctattggcca
     2461 atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca tggctggcta
     2521 ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc tgaaatgctt
     2581 gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt tccgtttggg
     2641 tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga tctggcaaat
     2701 gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg tgatggactc
     2761 gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac gacggctgct
     2821 aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac agcattggct
     2881 gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga accatgggta
     2941 ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca tttagctgaa
     3001 tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc agagaagatc
     3061 ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca ctttggcgaa
     3121 tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg tttagatatg
     3181 gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac aaacgaagat
     3241 acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa acaaatcaaa
     3301 tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg tcaaaaagta
     3361 gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca agaccgtcaa
     3421 atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg gaaaaaagtc
     3481 gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca aggaatgatc
     3541 ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa atccatgcca
     3601 aatggctcag aaatcgcata gaaatt
//
LOCUS       DACETN010000139         3390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DACETN010000139 DACETN010000000
VERSION     DACETN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.145"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            647..964
                     /locus_tag="IXF98_002504"
     CDS             647..964
                     /locus_tag="IXF98_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1556786.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IXF98_002505"
     CDS             complement(1055..1420)
                     /locus_tag="IXF98_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556787.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IXF98_002506"
     CDS             1628..2110
                     /locus_tag="IXF98_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556788.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IXF98_002507"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IXF98_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1556789.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1080 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac
//
LOCUS       DACETN010000140         3351 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DACETN010000140 DACETN010000000
VERSION     DACETN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.146"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..238
                     /locus_tag="IXF98_002508"
     CDS             <1..238
                     /locus_tag="IXF98_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556790.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IXF98_002509"
     CDS             252..440
                     /locus_tag="IXF98_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556791.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IXF98_002510"
     CDS             452..1063
                     /locus_tag="IXF98_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1556792.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IXF98_002511"
     CDS             1048..2439
                     /locus_tag="IXF98_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1556793.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IXF98_002512"
     CDS             2655..2957
                     /locus_tag="IXF98_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556794.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..3297
                     /locus_tag="IXF98_002513"
     CDS             2950..3297
                     /locus_tag="IXF98_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556795.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
BASE COUNT         1307 a          473 c          655 g          916 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa aggctcttcg tcaaatcgtg ttggtgaaga
     3301 caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgccgca t
//
LOCUS       DACETN010000141         3321 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DACETN010000141 DACETN010000000
VERSION     DACETN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.147"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            135..1103
                     /locus_tag="IXF98_002514"
     CDS             135..1103
                     /locus_tag="IXF98_002514"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1556796.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="IXF98_002515"
     CDS             complement(1420..1944)
                     /locus_tag="IXF98_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1556797.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..>3321
                     /gene="scm"
                     /locus_tag="IXF98_002516"
     CDS             2231..>3321
                     /gene="scm"
                     /locus_tag="IXF98_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1556798.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTT"
BASE COUNT         1059 a          674 c          615 g          973 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc
     3301 aagcgacagt acagatacta c
//
LOCUS       DACETN010000142         3279 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DACETN010000142 DACETN010000000
VERSION     DACETN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.148"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(862..1203)
                     /locus_tag="IXF98_002517"
     CDS             complement(862..1203)
                     /locus_tag="IXF98_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1556799.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IXF98_002518"
     CDS             complement(1203..1433)
                     /locus_tag="IXF98_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1556800.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IXF98_002519"
     CDS             1720..2058
                     /locus_tag="IXF98_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556801.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IXF98_002520"
     CDS             complement(2263..2502)
                     /locus_tag="IXF98_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1556802.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IXF98_002521"
     CDS             complement(2478..2825)
                     /locus_tag="IXF98_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1556803.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1036 a          602 c          506 g         1135 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttg
//
LOCUS       DACETN010000143         3266 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DACETN010000143 DACETN010000000
VERSION     DACETN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.149"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            484..771
                     /locus_tag="IXF98_002522"
     CDS             484..771
                     /locus_tag="IXF98_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556804.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            789..1211
                     /locus_tag="IXF98_002523"
     CDS             789..1211
                     /locus_tag="IXF98_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556805.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1231..2823
                     /locus_tag="IXF98_002524"
     CDS             1231..2823
                     /locus_tag="IXF98_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556806.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3079..>3266
                     /locus_tag="IXF98_002525"
     CDS             3079..>3266
                     /locus_tag="IXF98_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556807.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1310 a          500 c          628 g          828 t
ORIGIN      
        1 gaaaaaagta tataaaaaat gtttatggat cagctatatc accctaggta tagctgttaa
       61 agagatggga gaaaacatta accacgattc taacacaaac atcaagcaca taaaacattt
      121 aacactcctc ctttctggct gaagatttca accaaacagg aagacagcgt atcaattata
      181 gcatacactt tttcttaaat tcatttttca tatgacaaaa tatttccttc taatcgacaa
      241 agttagaagg attttttatt gtctaaatac acaaataacc ttaattttta aagagcataa
      301 ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta ttacgtttct
      361 ctaaaacaaa agtgggtaca aaatattcga tttatggata gtgtatggat agtaaaacag
      421 ctatacatgg atagcatatg gatagactcc atagactatc catacttttt tcggagggtg
      481 aaaatggaaa ttaaagagat taaagtaaga ggtattaata aaaaatatgt tcaagaaatt
      541 gatcatagat gcgtagaatt aacagaacaa actggtcaaa aatggagtcg aaatgattat
      601 ctaaaactat tgattgaaaa cgactttgaa cgtcctttaa tggactacaa gaaagatcaa
      661 tttgatcgtt tattggaaaa atttacagat gttcagctgc ataatacaaa agttttggaa
      721 gcatacacga acgaggtcaa gaacttaatt gaaatattaa tcgcacatta ggaaggagat
      781 aatgttctat ggttaaagcg atagaaaagc atattacatt ttatgaagcg cacgatgaag
      841 ttttaaaaca tattatggaa actcagccta cggtaaaaaa ctattctgaa gccattcgat
      901 ttttatgtat gaattacgat ccagaaattg aaaaaaataa taatatgacg aacgcaaagt
      961 tgaacgcaat gggaaaggaa atttctattt taatagaaat ggtttcagag tttgcggatc
     1021 ttcatttgaa agatacagga atattaattg gtacaaattc aactgtctat caagaagcaa
     1081 aagagcgtgt ggaagggaaa ataaaagcga atcaaacgaa gaagtattct cctaaaatca
     1141 agaaatcaat aaatgaatct gttcatgcca gagtagaccc gtttgatcca acaaagtttt
     1201 taaaaagata aaaagtagaa ggggaatatg atgaataaat cgcctgcagt cattctcact
     1261 tgtcaattta ccttgcccaa taaaaaatct ttttcaacat acattgatta tatgacaaga
     1321 gaaaaagcgc tggaagaaat ggaacgacgt acgcctgaag aagaacagga actgcaacga
     1381 atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc ggagagtaag
     1441 acggataaag aattcagcga aacgtcgaat gaagctcaat ctttgatggg aaatggaatc
     1501 aactttgaca agttagagga acaagatttt accaaatatc tttcttatat gacccgtcac
     1561 tatgcgttag aacaaaagaa agaactgacg caaagtgaac aaaaagaata ccaacgcttg
     1621 aaccaagcaa taaaaaaata tcaaacagag ccacacaaaa aagcttcagg aaaccttcct
     1681 ggagtttttt ctatggcttc agacgaagtg aaaggaagtg atctgaagga gattaagcaa
     1741 atcttccaaa gaggacaaca aaaaggctca atcatttatc aagatgttgt ctctcatgat
     1801 aattcgtatc ttgaaaaaat aggattatac gatcccaaaa cagatacttt agatgaagaa
     1861 ggattaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga gcaactgaac
     1921 gaaacaggct actggatggc aacgattcac cgtaatacca aacatatcca tattcatttt
     1981 gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa tggtgtgagt
     2041 tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa gaccaattat
     2101 gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat ggagaaacga
     2161 aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt aaaagagcca
     2221 gcgcgttacg atcgacacgc aatgaactta ttaaatgaag tatataaaac cctacctgac
     2281 aaacgttcgg aatggcatta tggggatgaa aaacgaacca aactttcacc atacacaaga
     2341 gagaaactcg atgagttaac caaatatgtt ttaacaaaag aacctgaata tcaagaatat
     2401 atacagattt caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga aagcaaaaga
     2461 gaatcaaagg atgcagaaaa aaatgctctc tttgatttga aaaagcgcac aggaaatgcc
     2521 attctaacag aactgaagaa acagaatagt caaataaaac ctttaataga aagtatggat
     2581 cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacagtt accaacgaat
     2641 gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca acaaagagtt
     2701 gtcacgcaac ggagtttgcg tcaattaaat cgaacgatta atgcccatga agaaaaatac
     2761 cacgcaagta aagcctacga agagcaacaa cgaagaatcc aaatagaaca agaaagacag
     2821 taaagacagt aaagttagcg ccttattata ctacttggga aatgaatgaa gaggggatca
     2881 ataacttccc gtgtaaaagc attgaagagt ctatttcact cacagaatgt tagttgttaa
     2941 gtgaataaaa gaagtaaaag agtcctgaac gtaattgtcc aggactcttt ttttcaatga
     3001 attgttttcc gccaattcaa tgagtggtgc gatttgcgac tacattatat caaaagaaag
     3061 agaagggagg aagaagaaat ggcttattca acgattggag ttgttgttga aaaaagcaga
     3121 gacaatctcg tttttgtgac agaaattcaa acaggcagag cttttgtcgt cacagacaaa
     3181 gcggcaaaag cgtatcaaaa tggcgatatt ttaacgttaa atctgactac gaagacgttc
     3241 gtcgatgcgg cagaagatta tccatt
//
LOCUS       DACETN010000144         3261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DACETN010000144 DACETN010000000
VERSION     DACETN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.150"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(368..1486)
                     /locus_tag="IXF98_002526"
     CDS             complement(368..1486)
                     /locus_tag="IXF98_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556808.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..>3261
                     /locus_tag="IXF98_002527"
     CDS             1957..>3261
                     /locus_tag="IXF98_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1556809.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1019 a          678 c          588 g          976 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt t
//
LOCUS       DACETN010000145         3234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DACETN010000145 DACETN010000000
VERSION     DACETN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.151"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            183..1137
                     /locus_tag="IXF98_002528"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IXF98_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IXF98_002529"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IXF98_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IXF98_002530"
     CDS             2184..2663
                     /locus_tag="IXF98_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1556810.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3234
                     /locus_tag="IXF98_002531"
     CDS             2679..>3234
                     /locus_tag="IXF98_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1556811.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT          979 a          540 c          751 g          964 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc gctt
//
LOCUS       DACETN010000146         3075 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DACETN010000146 DACETN010000000
VERSION     DACETN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.152"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(109..1044)
                     /gene="murB"
                     /locus_tag="IXF98_002532"
     CDS             complement(109..1044)
                     /gene="murB"
                     /locus_tag="IXF98_002532"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1556812.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1117..2037)
                     /locus_tag="IXF98_002533"
     CDS             complement(1117..2037)
                     /locus_tag="IXF98_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1556813.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2150..2902
                     /gene="xth"
                     /locus_tag="IXF98_002534"
     CDS             2150..2902
                     /gene="xth"
                     /locus_tag="IXF98_002534"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1556814.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          873 a          623 c          533 g         1046 t
ORIGIN      
        1 ttattgtgtt aaaatgtaca agatttgcgc catttgcgta aacaaccaca aaaaagagca
       61 ggacggccac cgtcctgctc ttttacttat tttaagttcc attacttatt aattggctaa
      121 acttctgctt gtttctgata ttgcttgttt ttctccaatg atccgcactt ctgtttctaa
      181 agacacatca aatttttctt tgattacttc ttggatgtgc gcaatcaatt caatataatc
      241 tgtcgctgtc gcatgatcga tattgacgat gaatccagca tgtttttcag agacttgtgc
      301 accgccccat ttcaggcctt gcaatcctgc ttgttggatc agctgacctg tatagtgtcc
      361 tactgggcgc ttaaagacac ttccgcatga agggtattct aatggctgtt ttgattgacg
      421 caattcggtc aactcatcca ttcttgcttt gattgcttca taatcccctg tttgcaaact
      481 gaatcgagct tccaatacga tagcacgtaa ttcttggact ttactgtgac gataagaaaa
      541 taccatttcg tcttttgtca aagttttcaa tgttccatct tcaagcaaaa gatccacttc
      601 agcaaaaaca tctttgatct ctccgccata cgcacctgca ttcatataaa ccgctccacc
      661 gacacttccc gggatgccgc aagcaaattc aaatccagtc aatgattctg ccaaagctgc
      721 ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg accatcgttc ctttgacatg
      781 aatctgtttc atttcagtca gcatgatgac tacaccgcga atcccgccat cttgaacgat
      841 cagattgctt gcatttccta ataccatcca aggtatatca tggattcggc aataatcgat
      901 gatttgcttt acttcttctt gttttttcgg gaatgcaagg acatcagctg gtccacctgt
      961 tttcgtaaac gtatagttca tcaatggttc atcaaacaat atagtaatcg tggctaagct
     1021 ttcaactatt tcttctttat tcactgagga ttttcctttc ttttctcaaa cgtcctcatt
     1081 attctatcac gcttaataca atcttgccag tcttgttcaa agttgatgac ggtagtgatc
     1141 tttaagcact tcctcacata attcatgact tcttacaact tctttttgtt taagcaattc
     1201 tgtttccgat ttacctaaac ggatctgaga aaagaaggcc tctaccattg gctcaaaccc
     1261 gcgtttgact agcgtgtttg tccagtcgcc aaattctttc actttttttc catcagcatc
     1321 ttgaatgaca agctgggtca gatcgtttac ttcataagta ccatttttac ttgttacttg
     1381 atatttttct atattagcgc cacttgctaa atccatcgtt aaaatagccg tttgattttg
     1441 tgtttctaat tgtaaaatgg catattccaa aaattccttt gtttctctta tctggtattt
     1501 tgttctcaaa atgggttcat ctaataaata cacagctgta tcaataagat gtaaaaacaa
     1561 atcataaagg acgaactctg tcgtttcttt tgccgctatt cgattttttt gcaggtggat
     1621 caaccgctta tctgggatac gtttcaattc ttctaccata ggagcaaagc gccggttgaa
     1681 accgatcatc aaaattattt ggttcttatc agctaattct tgcaattcac gaacttcatt
     1741 tagatggaca ctcagtggtt tgtcaatata aacatggata ccattttcta gacatttttt
     1801 ggctaattca taatgtactt ttgttgctgc gtggatcata catgcttcga ttttttcttc
     1861 taaaagctca tcgatcgttt catatagatg ttggaacccg tactcttttt gaattctttc
     1921 ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt gttccttgtt ttttcgcata
     1981 agttggtaaa tatgcttttt ctgcgatatt ccctacaccg attacaccaa tcttcatcta
     2041 ctttcctcct ggatcattta gttactttca tcttacacca ttttttacta aaatgaggta
     2101 caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt gtgcttttat tgaaatttat
     2161 ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa aattttttag aagtttttca
     2221 tgagttagac gccgattttt tctgtctgca ggaaacgaaa ctccaagcag gacaaattga
     2281 tttagatctt ccaggttatt atcaatattg gaattacgcg gaacgaaaag gctattccgg
     2341 cacagcgatt tttgctaaaa aaccagcatt gaatgccact tacggaatgg gtatcgatat
     2401 ccatgataca gaaggccgtc tgattacttt ggaatattcc gatttctttc ttgtaacctg
     2461 ttacacgcca aattctcaaa gcgaattaaa acgtttagat taccgattag aatgggaaga
     2521 agccttttat aattatttgg aaaatctaaa aaaacaaaaa ccagtcatcg tgtgtgggga
     2581 tctaaatgtc gctcatcaaa agattgattt gaaaaactgg aaaacgaatc aaaaaaatgc
     2641 tggattcact ccagaagaaa gagcggctct ttctcgactg ttggataacg ggtttatcga
     2701 tacattccgt tatttttatc caacccagga aggtgtctac tcttggtgga actaccgatt
     2761 caattcaagg aaaaacaatg ctggttggcg gatcgattac tttttgacga gcaaagattt
     2821 agagccagga ctagctgatg caaagatcca tacggacatt atgggcagtg atcattgtcc
     2881 agtagaatta gatttgaaat aaagaggttg tgaaaaagtc gtttagtttc gagtattaag
     2941 aaaaattttg tgaaaatggc tttccacatt ttgcataaat ttggcataat accgagaaac
     3001 tggcttttga acactgttta tcgagagatt gtgaaaaagc tgtcctgcat caagtaataa
     3061 gaacagccaa acaaa
//
LOCUS       DACETN010000147         2992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DACETN010000147 DACETN010000000
VERSION     DACETN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.153"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(106..1053)
                     /locus_tag="IXF98_002535"
     CDS             complement(106..1053)
                     /locus_tag="IXF98_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1556815.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IXF98_002536"
     CDS             1299..1763
                     /locus_tag="IXF98_002536"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1556816.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IXF98_002537"
     CDS             complement(1825..2868)
                     /locus_tag="IXF98_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1556817.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACETN010000148         2924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DACETN010000148 DACETN010000000
VERSION     DACETN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.154"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            441..1400
                     /locus_tag="IXF98_002538"
     CDS             441..1400
                     /locus_tag="IXF98_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556818.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1387..1992)
                     /locus_tag="IXF98_002539"
     CDS             complement(1387..1992)
                     /locus_tag="IXF98_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556819.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2232..>2924)
                     /gene="tnpB"
                     /locus_tag="IXF98_002540"
     CDS             complement(2232..>2924)
                     /gene="tnpB"
                     /locus_tag="IXF98_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1556820.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          939 a          501 c          461 g         1023 t
ORIGIN      
        1 tttgcttttc actaattaca aaatatgtga tataggaagt ttagttattg ttctcataat
       61 gccatcctgt ttttagatta tctagtaatt tttttgttat ttaaaattag gagcagtgcc
      121 aattgtttta attttaaagg ctttttttat atcttctaaa ttagaagata tttgtatatc
      181 aatatggaat atattacctt ttttgtattt ttttatgtta tctttagcta aaacatcaaa
      241 tacatgtcct tgaaattgta tcgaggcagc tggatttagt ttaaattgag gaaactttac
      301 cacagctgat tcaaaacact tgtcacattt ttttattttt ttaccttcgc cgattgtaaa
      361 attaagctag acaactaaaa aagtcatttg tgctacactc aaaatagttc cgtcgaaaga
      421 attataagga gtgagtacaa atgacctata cccatcttac accaactgaa ctcgtaatga
      481 tagaagctta tttcaatcaa tcacactctg tttcaaaagt agcccatctg gtacaacgtt
      541 ctcgacaaac catttataat gtctatcgtt ttcttaaatc tggtggaagc gcaataagct
      601 actatgaaca atataagaaa aacaaacgta actgcggaag acgtccgatt gtgttaccag
      661 atgggcaaca agaatacatt caaaaaaagg tggctcaagg gtggactcca gatgtcctta
      721 ttggtcgagc agagtttcct attgcttgtt cggtacgtac actatatcgg atgtttaaag
      781 ataaaacctt tgaccctcac gatttaccaa tgaaagggaa aagaaaacca aacggacata
      841 aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca ggatagaaca actgattata
      901 tacagttcaa tcatgagttt ggtcacctag aaggtgacac cattgtcggt ggaaaacata
      961 aaagtgcggt gatcacgtta gttgagcgat tatctaaagt gatcatcacc ttgaaacccg
     1021 ctggtaggca agctatagat attgagaata gactgaatca atggtttcaa tctgtaccga
     1081 aacacttgtt taaatccatc acatttgatt gtggaaaaga gttctcgaat tggaaaaaga
     1141 ttagtaatac caatgatatt tcgatttatt tcgctgatcc aggaacacct tctcaacgag
     1201 gactaaatga acattcgaat ggcttattac gtaaagatgg cttatataaa gctatggatt
     1261 ttaataaggt atccgaaacc tttatccaat cggtggcatc aaaacgaaat catattccta
     1321 gaaaatcatt acactatcgc acaccattgg aagtattttt gagttacgta ggtaaagatg
     1381 atttgtctag cttaatttga caaacgaaac tttgtaaaaa tattcgattg aatattctat
     1441 ttggcacgac tcacttggca gtatatgtat gtcaatattt ttcatctgtt tcataggtat
     1501 ttcaaaatct aaactattaa aaacatataa ttccctgtaa ttatttacat ctaactctac
     1561 taaatttatc tttggaacaa tagatcctcc acccattcca ccataataaa aaaaatcttg
     1621 ttctctatca aaatagcaca tattaagctc agtttgttta tcatcctcaa taattttata
     1681 aatcaaacct atataatcat gaatgctggc aaaaattttt tttaagtcaa gttctaaatc
     1741 ttcttcgaaa acacatttaa tttctcctac tgataattga tactctatat tacatgactt
     1801 aattagtttt ttagatcccc cctccagata ggcatatgtt tcaataatta tgttcttttt
     1861 attgcttggc aaatttccgt tatttattgc ataaattaaa aatgactgtc tatttagccc
     1921 ataatctaat acaacttctt catactcata tttctttaaa tatttaatat gaaaatatgc
     1981 tgaattaatc aaaactcgag tgttactgtt gttatttatt gttgttctat tgtgtataaa
     2041 attgtatagg ttgtcatatt ttcttggttt agtgaactac tcaccactta gctaaaccta
     2101 acggtttctt acgtttgaag tgggagcttc ttgggaagag agcatacttg atagcgtatt
     2161 actttactaa cagaggtgcc tcttatttac caagctatcc ccgtagttcc tacggttctt
     2221 gtttatttga tctaagccaa tcccatagag tgtagcctta gaccttcggt caggatattg
     2281 atactagcgt tgatatctcg gtcatgatga gtatgacaaa tagggcaagt ccattttcga
     2341 atttcgagag atttcttgcc gtctttatgt ccacaattcg aacagatttg gctagatgga
     2401 aaccacttat ctactttgat gattttccga ccgtaccagt cagccttgta ttgtaattta
     2461 gacacaaaac tagaccaaga aacatccgaa atactttttg ctagtttatg attacgcaac
     2521 atgccttttg tgtttaagtc ttcaatacag atgatatcgt gatttttgat aatttctgta
     2581 ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa tcgtgctact
     2641 ttacgttttt gtgtttgata atttctagct tcaaacagat tgataccttt ttgtttagct
     2701 aataaggcac gtcttgacaa cttacgttgt tcacgtttga gtttcttttc cattttggac
     2761 gtaaatttat tattatcaaa tttttgaccg gtagaaagaa ttgcaaagtc tataatacct
     2821 aggtcaatac caacagcaga attagattta ggtagttcgc gaacttcttc tttgcataac
     2881 aaagaaatat aatatttacc gctagaatga cgtgatattg tagc
//
LOCUS       DACETN010000149         2902 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DACETN010000149 DACETN010000000
VERSION     DACETN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.155"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1141
                     /locus_tag="IXF98_002541"
     CDS             <1..1141
                     /locus_tag="IXF98_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1556821.1"
                     /translation="EIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGV
                     IEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPK
                     GTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVT
                     TDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVT
                     ISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKV
                     PKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNR
                     GKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1275..2618
                     /locus_tag="IXF98_002542"
     CDS             1275..2618
                     /locus_tag="IXF98_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1556822.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2730..>2902
                     /locus_tag="IXF98_002543"
     CDS             2730..>2902
                     /locus_tag="IXF98_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1556823.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1154 a          387 c          541 g          820 t
ORIGIN      
        1 agaaattgga ggcacagtag atttaacaga taatgttact aacttgccag cgttaccaga
       61 aggaacaaca gtaacagatg ttactcccgg cggtacaatt gataccaaca caccaggcaa
      121 ttatgaaggt gtcattgaag taacgtatcc agacggtacg aaagatacgg tgaaagttcc
      181 agtagaagtg acagacaatc gttctaatgc agataaatac aatcctgaag gacaaaaagt
      241 gactacagac ttaaataaag agccagatgc atctgaggga attaagaaca aagaagattt
      301 accaaaagga actacttatg cttggaaaga gaaagtagat gttagcacag ctggaaataa
      361 aaaaggtact attgtagtga catatccaga tggatcatct gatgaagttg aagtagacgt
      421 tacagtgaca gatactcgtt ccgatgcaga taaatataat cctgaaggac aaaaagtgac
      481 tacagactta aataaagagc cagatgcatc tgagggaatt aagaacaaag aagatctacc
      541 aaaaggaact acttatactt ggaaagaaaa agtagatgtt agtacagctg gaaacaaaaa
      601 aggtactgta gtagtaacat atccagatgg atctaaagaa gaagtagaag ttactatttc
      661 tgtagtagat aaaaaagcac cgaataaacc tcaagttgat cctattacag agggtgacca
      721 aattgttact ggtaaaactg aaccaaatgc agaggtgaca gttacattac cagacggaag
      781 tcaacaccat gggacagctg ataaaaatgg taattttaca gttaaagttc ctaaattaga
      841 ggcagggaca aaagtgatag taactgcaac tgatgaatct ggtaatacta gtgaacctac
      901 taatgtagtt gtttcttcca acaaaaaaga cagtggaaaa aacggtagta aaggtagtaa
      961 gactgacaat caaggtagca acagcaatca agacaaaaat agaggcaaaa gccagtcctc
     1021 aaaagttttt cctaagacgg gagagagtga cagtaatatc tttactatta gcggtggttt
     1081 aatactgtta ggaacgttag gattattagg gtataaaaat cgaaaaaaag agaatgaata
     1141 gaaaatcaat ttcttaagtc ttctctgaga agaggaagaa tctttttctt cctctttttg
     1201 cttttttatg atgggaaata agtgttagtt aaagatgaat atacttttta tagaaaggaa
     1261 aagaatgtaa ttaagtggaa aaaatagtaa ttattggttc aaaccattca gggctagccg
     1321 cagctcgatt tctaaactta agcgattcaa attatgaagt tattctaatc gataaaaaat
     1381 cagaaattag ttacttggaa tgtgggacat cgttatttat aaaaggagaa gtaagtaatt
     1441 tagaagatct tttttatcaa aatagaatga cactaaattc tgagataaaa aagttatttc
     1501 tagagacaga agttgacaaa attgatattc aaaataaaaa gattagttgt gtaaaaaaag
     1561 atggtacaag attctctcaa gaatatgaca aagttatatt agctacaggt tcaaagcaaa
     1621 ttatgctaga tataccgggg aataatttag ctaatatttt ttcgataaag aattttccaa
     1681 atgcaaaaga gatttttaaa gctttggatg atagtactat taaaaaagta ggaattattg
     1741 gagcaggata tattggagtt gaaattgctg aagccataag aaaaagaggg aaagaagtat
     1801 atcttttcga tgttgcagat agagtgttgt cgacttatta cgaccgtagt ttttcagata
     1861 aagttgaaga aatactatca aaaaatggaa tacatctttg tctatcagaa agtcctaaaa
     1921 aatatttagg aagaaaaaag attgaaaaaa tagtcactac aagtggtacc caatactcta
     1981 tggatatggt cgtacaagct ataggatttc tatctaatag tccaattggg gaaaaggaac
     2041 tcttgagaga tgttagtgga gcatatctaa ctaatgaata ccagcaaaca aatataaaag
     2101 atatatatgc tattggggat tgtgctacaa ctttttttac gtccattaat agaagtagtg
     2161 ttgatttttc tatttctaat gctctaagaa cagcttatat tgcaactcaa catattaaca
     2221 atcaagattt tacaccttct ggttcccaat ttacaaatgg cttttctata tttaattaca
     2281 atttttacgc agcaggtttg aatttaaaaa cagcacgatt attaagtgag tctatagatt
     2341 atatagaaat agaagatttc attagacctg cttttttgag aaaaaatagt cgtgtaaaat
     2401 taagaattag ctttgaaaaa aatacaaaga gaatagtagg tgtacaactt tgtagtcaag
     2461 aagatttatc tggactatta tcaatgtttt ctcttgctat agaagaacaa attacattag
     2521 acaaattgaa aatgttagat tatttatttc atcctagttt tagccaaccc tataatttta
     2581 ttttaaaggc agtaacagga ttaaacattg aaacataatc gttactaaat gaaatcaaga
     2641 aatatactgt attactcaat tcattgaaaa tatttatcat aatttttaat agaaaacact
     2701 attaaagaga aaaaagagag gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag
     2761 ttatattaat gtcccaactc cttatagcaa cacctttcca tgtgttggca agcgaaaatt
     2821 caacatacaa aattgcaaaa gaaagaaccg agacgactga aatgagtaat tccgatgtaa
     2881 cagcagagag tagtgaactt tt
//
LOCUS       DACETN010000150         2844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DACETN010000150 DACETN010000000
VERSION     DACETN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.156"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(22..2631)
                     /gene="clpB"
                     /locus_tag="IXF98_002544"
     CDS             complement(22..2631)
                     /gene="clpB"
                     /locus_tag="IXF98_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1556824.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT          735 a          623 c          478 g         1008 t
ORIGIN      
        1 ttttatagtc tcaatggaag attagacgat ttcttctagc tcttctgttt tgaaatgaag
       61 ttgtccatca agtaaagtaa tcgttacttt tgatttaggc ataacatgtc ctgcaacgat
      121 ttctttagct agtggtgttt ctacttcctt agtgataaat cgtttcaatg gtcttgcacc
      181 atatgctggt tcataagcat tttcagcaat ccacgttttg gcttcatcac tgattgtcag
      241 caggatttct tgatgttcca gacgttgtgc caattgcgcc accattttgt caacgattcc
      301 tttcacgttg tctaggctca atggtgtaaa taaaatcgtg tcgtcgattc ggttcaagaa
      361 ttctggtttg aagtttccac gtaatagcgt gttgacttgt tcagccactg cttctggaat
      421 cgttccatca gcagtcacgc cttccagcag taactgtgaa ccgatattgc tggtcatgat
      481 cagaaccgta tttttgaaat ctactactcg cccttttgaa tcagtcaagc gtccgtcatc
      541 taggacttgt aacaagatat tgaagacatc gggatgcgct ttttcgattt catccaacaa
      601 gacgattgta taaggatttc gtctgacagc ttccgttaat tgaccacctt cttcgtaacc
      661 tacatagcct ggaggagctc cgactagacg agacacagca tgtttttcca tatattcact
      721 catatcgatc cgtaccatat gatcttctga gtcaaacaag ttctcagcta acgctttcgc
      781 taattcggtt tttccgacac cagttggtcc taggaaaagg aaagaaccta gaggacgatt
      841 tgggtcttgc aatccagccc gtgaacgaat taccgcgtca ctgaccgcat caactgcttc
      901 atcttgacca atcactcgtt tatgaagtgt ttcattcaat ttaattagtt tttctcgttc
      961 accttctaca agtttcgtta cggggatacc cgttaatctg ccaacgactt gagcaatctc
     1021 attttcggta acggactctt gaaccatctt aatctcgctg tcttttgctt tagcttctaa
     1081 ttccttcaat tctttttcta attgaggtat cgtaccatga cgcaatacag cagcgcgttc
     1141 taaatcatag ttattttctg catcttctaa ttcgtgtttt gctttatcga tttcagcacg
     1201 ttttgctgaa acagaattga cttcttcttt ttctgtttcc cactgcatct tcatcgcatt
     1261 tgcttcttca cgaagttcag ctaattcttc ttgcaaattt ttcaagcgtt ttttacttgc
     1321 atcatctgat tcttttttca gtgcagcttc ttcgatttcc aactgcatca aacgtctagt
     1381 aacttgatct aattccgttg gcatagagtt catttctacc cgaatcgttg cactggcttc
     1441 atcgatcaaa tcgattgctt tgtccggtaa gaaccgatca gtaatataac ggtcagataa
     1501 agtagctgct gctactaatg cgttgtcatg gatgttaaca ccatggtgga tctcaaaacg
     1561 ttcttttaat ccacgcagaa tactgatcgt atcttccact gttggttctt tgactaatac
     1621 cttttgaaag cgacgttcca acgctttgtc tttttccata tattgacggt actcatctaa
     1681 agttgtggca ccgatcaaat gtaattcacc gcgagcaagc attggtttca acagatttcc
     1741 ggcatccata cttccttctg tttttcctgc ccctacgata ttgtggattt catcgatgaa
     1801 caagaggata cgtccatcac tttttttgac ttctttcaag actgctttta gtctttcttc
     1861 aaattcacca cggaattttg ctccagcgat caatgcaccc atatctaatg aaaaaatcgt
     1921 tttatctttt aaattttcag gcacatcttt gcgtacgata cgttgagcaa gcccttcaac
     1981 gattgccgtt ttccctacac cgggttctcc tatcagaaca ggattatttt ttgtttttcg
     2041 tgaaagaata cggatgacat cacggatttc ttcatcacga ccgatgattg gatccatttt
     2101 tccgcttttg acttgctgta ctaaatcaac gccgtatttt tctaatgctt tgtattgttc
     2161 ttcttggttt tgtgaggtca ctctttctcc tcctctcatc tcttcgatgt ttttacgcaa
     2221 ttctttttca gataatccgt ttttattcaa ataaacagtt aacggataat tttttaattt
     2281 catcaacgct aaaataacga cttccgttga aagaaactcg tcgccgaagc tttcacgtat
     2341 ttggtctgct tcattcaata atcgaaagaa attctggctg aggttttgtc catattggac
     2401 gtttccacca gaaacaactg gatactcatc cagtagacga tcaatttcat gttcaaaaga
     2461 ttcgacatcc aatccggcat ccgtatagaa atttcgacca aaatgattag attgtaaaaa
     2521 gattttccat acatgggcaa tgtcgatatc ttgatgtttt cgagtgaccg caatctgttg
     2581 agcttcagca atcgcttctt gaagcgtcgt agtcattttc tcaatattca tacaggtata
     2641 ccctcctaat taaaggtaag atcacttatt tctttgatct ttacaaaaac tattatatcg
     2701 ctttggtcaa ttttggtcaa aatatacaca tagttttttg actttttttc tcgacttata
     2761 taaatcttaa aaaaaataaa attgattgta tgtatataaa aaaagagcca aacaaacgta
     2821 aaattatctt atccataacg tttt
//
LOCUS       DACETN010000151         2842 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DACETN010000151 DACETN010000000
VERSION     DACETN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.157"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            95..370
                     /locus_tag="IXF98_002545"
     CDS             95..370
                     /locus_tag="IXF98_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1556825.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            808..1758
                     /gene="yidC"
                     /locus_tag="IXF98_002546"
     CDS             808..1758
                     /gene="yidC"
                     /locus_tag="IXF98_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1556826.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1937..2137)
                     /locus_tag="IXF98_002547"
     CDS             complement(1937..2137)
                     /locus_tag="IXF98_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556827.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          892 a          564 c          523 g          863 t
ORIGIN      
        1 ccatgattat atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca
       61 ttaatttata aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca
      121 aggacgcgtc caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg
      181 cgtgactgga acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa
      241 tgacgacatc atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg
      301 agtgcagtat gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt
      361 ggtgggataa ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa
      421 atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca
      481 ccgtcgataa ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa
      541 gctgatctat cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt
      601 tttagtttat taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc
      661 tttcttgatt ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat
      721 atgaaatttc tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg
      781 taaaaattat agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc
      841 ggacttgtca gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac
      901 cctgacactt cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca
      961 tatctagttg aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc
     1021 gtccgtatca tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct
     1081 gaaaaaatgc aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca
     1141 acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac
     1201 ggattgagta tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc
     1261 tctgccttgt actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc
     1321 atcgatttag gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt
     1381 caaggataca tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg
     1441 ctgatcgtct ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca
     1501 ctttactggg tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg
     1561 atgaaaccac gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa
     1621 gtcgtcactc caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca
     1681 accaaacaaa tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag
     1741 cagcagaagc ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag
     1801 caattaaaaa tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag
     1861 tgaatgttta atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg
     1921 aagaaacaac tttttcttat ctatcaacgt agacaggtgt tgctgtttct tctaatagtg
     1981 tacttaattc cttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt
     2041 cgccttcggt aacaaaaggc gccactccct tcgcccatgt tgctcgccca cataagacac
     2101 cattctccat aaaggacaac gcggagatac cacccatcta aataacatcc ataaaccttt
     2161 ctagtagaag aatagtgata gtatccatcc ctttttctac tgggaataca attacacgat
     2221 tcaaagcttg atacacaaca tttcccttct tataaaccgc tattgtaacc gaattgtttt
     2281 gaatcacctt tttgggatag aaactggctc ctttccgcaa cttcttaaat gttggtttct
     2341 tttatacttt ctttgtagca actacaaatt tcttcaaatc tctaaatgtt tgcataacaa
     2401 ccgcctttct gacttttact caggatggta tttaaaaatc agcaaaataa ggcttctctc
     2461 catctttttt atttcctagc tgctttaaag acactcttgc tgttttttaa tctctatcaa
     2521 gggggctctg aatctatttt gatgaactta tcttattaat ttctcaaatg agctgtcaga
     2581 cacaactgta ttttttcaat ctcataccgg acaactatgc cagcttaaaa aaatttcctc
     2641 tggcaaaact tctttattga aaaatgacaa tgaaaaaaga ccattctttt tgttgaatgc
     2701 tcattgtgta aacatatttc ctgcgactgc tcttcgaacc attctcagtg caatccctaa
     2761 tttatctatt aaatcttctt ttagttcctc atctttcata ttcaaaattt cctttgtatg
     2821 ttacacaagt ttgttctcta aa
//
LOCUS       DACETN010000152         2778 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DACETN010000152 DACETN010000000
VERSION     DACETN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.158"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..197
                     /locus_tag="IXF98_002548"
     CDS             <1..197
                     /locus_tag="IXF98_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556828.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXF98_002549"
     CDS             314..811
                     /locus_tag="IXF98_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1556829.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXF98_002550"
     CDS             898..1290
                     /locus_tag="IXF98_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1556830.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IXF98_002551"
     CDS             1274..>2778
                     /locus_tag="IXF98_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1556831.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACETN010000153         2773 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DACETN010000153 DACETN010000000
VERSION     DACETN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.159"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            178..774
                     /locus_tag="IXF98_002552"
     CDS             178..774
                     /locus_tag="IXF98_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1556832.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IXF98_002553"
     CDS             898..2697
                     /locus_tag="IXF98_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1556833.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          790 a          479 c          523 g          981 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt ttt
//
LOCUS       DACETN010000154         2650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DACETN010000154 DACETN010000000
VERSION     DACETN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.160"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            36..197
                     /locus_tag="IXF98_002554"
     CDS             36..197
                     /locus_tag="IXF98_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556834.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IXF98_002555"
     CDS             210..713
                     /locus_tag="IXF98_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1556835.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IXF98_002556"
     CDS             773..1162
                     /locus_tag="IXF98_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1556836.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IXF98_002557"
     CDS             1149..>2650
                     /locus_tag="IXF98_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1556837.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACETN010000155         2627 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DACETN010000155 DACETN010000000
VERSION     DACETN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.161"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IXF98_002558"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IXF98_002558"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1556838.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          459 c          581 g          644 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaact
//
LOCUS       DACETN010000156         2401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DACETN010000156 DACETN010000000
VERSION     DACETN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.162"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(330..647)
                     /locus_tag="IXF98_002559"
     CDS             complement(330..647)
                     /locus_tag="IXF98_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1556839.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(648..902)
                     /locus_tag="IXF98_002560"
     CDS             complement(648..902)
                     /locus_tag="IXF98_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1556840.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1162..1380)
                     /locus_tag="IXF98_002561"
                     /pseudo
     CDS             complement(1162..1380)
                     /locus_tag="IXF98_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          787 a          436 c          347 g          831 t
ORIGIN      
        1 aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag taaagtcacg
       61 aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc agtgtctctt
      121 attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga ctaatgattg
      181 tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag tcttatatat
      241 caatcttcga gtaaccgacg gttaacaacc atatcgagcg gcattaatca cccacctgta
      301 gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg tttagattgg
      361 aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa taaggcatag
      421 cttttgccga tcacgatccg atatgttggg atatccattc catagatttc ggaaacttcc
      481 tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc acctacaaat
      541 ctttttatgc tggctggaga cagagcaagc tcattttccc attcttctat tatcgtttca
      601 aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta ttcactccag
      661 ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc tttctcaaag
      721 tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg agtagtatct
      781 aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt taatccaaga
      841 gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag ttcttttccc
      901 atgttatcac actcctttta gtttttgata aataaattat aacatgttat aacaacaatt
      961 accaacggtg tttcttaagg gattccactc catattatta ttggacaaaa taagctactt
     1021 agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc accatacact
     1081 tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca tatgtcggtc
     1141 ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta cacgaataaa
     1201 gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata gcaatactgg
     1261 aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg catacttaaa
     1321 tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa atggatcaat
     1381 agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac gttaaaaaat
     1441 attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca aaactgatcg
     1501 atattgtatc caaaaatata tactacctaa aataggtagt atatattaaa atatgtgctt
     1561 ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag gtattttagt
     1621 tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg gaggaggaga
     1681 tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag aggtaactgc
     1741 acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta agcctacccg
     1801 ttaattctat aatatagaaa cgtaaaaaaa aaccccatca gcctgcacgc tgatggggtt
     1861 tggtgatata atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg
     1921 caaccaatag aaaaaactac ttagaacggt agatcatcgc taataacaat gcattgtcca
     1981 ccaaatggat tttgtgaagc attaaataca ttgttattaa ttatcgctgc ttttaactag
     2041 gcctctcccc ttctattata aaagaagata tatatcattt cttaattacc tactacctta
     2101 aaccaaaatt taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat
     2161 cccaattgtt atgattttat aatacgatta tttgtaacaa aatgaatatt atataattaa
     2221 acactaccat atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc
     2281 tcttaacttc aactaagtta tgctgcacat ctttatataa atcatatgca actcattagc
     2341 tagacaaact acaagcatgt agttttatct gttaattatg tactacctaa ataaagtagt
     2401 t
//
LOCUS       DACETN010000157         2399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DACETN010000157 DACETN010000000
VERSION     DACETN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.163"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            208..2070
                     /locus_tag="IXF98_002562"
     CDS             208..2070
                     /locus_tag="IXF98_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1556841.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2399
                     /locus_tag="IXF98_002563"
     CDS             2070..>2399
                     /locus_tag="IXF98_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1556842.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DACETN010000158         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DACETN010000158 DACETN010000000
VERSION     DACETN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.165"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(51..1598)
                     /locus_tag="IXF98_002564"
     CDS             complement(51..1598)
                     /locus_tag="IXF98_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1556843.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXF98_002565"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXF98_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1556844.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IXF98_002566"
     CDS             complement(2043..2237)
                     /locus_tag="IXF98_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556845.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          476 c          408 g          765 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ttcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACETN010000159         2211 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DACETN010000159 DACETN010000000
VERSION     DACETN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.166"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IXF98_002567"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IXF98_002567"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1556846.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          588 a          479 c          405 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACETN010000160         2125 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DACETN010000160 DACETN010000000
VERSION     DACETN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.167"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..159
                     /locus_tag="IXF98_002568"
     CDS             <1..159
                     /locus_tag="IXF98_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1556847.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="IXF98_002569"
     CDS             242..859
                     /locus_tag="IXF98_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556848.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="IXF98_002570"
     CDS             1023..1355
                     /locus_tag="IXF98_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556849.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="IXF98_002571"
     CDS             1679..1840
                     /locus_tag="IXF98_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556850.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="IXF98_002572"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="IXF98_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DACETN010000161         2118 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DACETN010000161 DACETN010000000
VERSION     DACETN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.168"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            908..1381
                     /locus_tag="IXF98_002573"
     CDS             908..1381
                     /locus_tag="IXF98_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1556851.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1390..1617
                     /locus_tag="IXF98_002574"
     CDS             1390..1617
                     /locus_tag="IXF98_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556852.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          679 a          339 c          315 g          785 t
ORIGIN      
        1 tttaatgcgt tcttacttta tttttatatt atcatttttc ttattcactt ctttggttta
       61 tactattctt ataaataaaa atgaggtatg tcctatgatg aaaagattgt actattcatt
      121 gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat ttggtatcag
      181 taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca tcttcgtatt
      241 tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg cacatgtttt
      301 aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct tcttaatacc
      361 tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat taagctattt
      421 cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat aacattgtat
      481 aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga aaaattctga
      541 tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc
      601 ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata aaaaatcctc
      661 caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa gagacacaca
      721 tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca
      781 ctccacacct tcatactcta tcatttttta taaacgatat atttttattg tttttcgata
      841 aaaaatagtt gttttacaat aattattctg ctatatttta gctatcaaag ataaaagagg
      901 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
      961 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
     1021 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
     1081 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
     1141 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
     1201 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
     1261 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
     1321 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
     1381 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
     1441 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
     1501 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
     1561 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
     1621 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
     1681 agttatttac gtctattatt cagatttttt atttcgattg aataccgttt tatctataaa
     1741 taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact atcacttttc
     1801 ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat aatactttat
     1861 tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt attacaggcc
     1921 attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg tgttaactct
     1981 tactccattc ttttttagta agctcccata tatactgtaa agccttctat atattatgtc
     2041 atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata acagtgatgg
     2101 acaattcttt tttatttt
//
LOCUS       DACETN010000162         2071 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DACETN010000162 DACETN010000000
VERSION     DACETN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.169"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(450..1064)
                     /locus_tag="IXF98_002575"
     CDS             complement(450..1064)
                     /locus_tag="IXF98_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1556853.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1670..2047)
                     /locus_tag="IXF98_002576"
     CDS             complement(1670..2047)
                     /locus_tag="IXF98_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556854.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          686 a          403 c          318 g          664 t
ORIGIN      
        1 atccttcaat ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt ggacaatttt
       61 gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat tactttgtaa
      121 agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag aatccatatg
      181 gattctttct tgtctctttt tccactactt tttatttttc tgacactaca tattcttata
      241 actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa caaaacaagc
      301 ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt cgttgttaat
      361 cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga acattatttt
      421 tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg gactagctgt
      481 tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg
      541 cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt ggcgtggttg
      601 tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg cgatttgata
      661 cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc tttgatccac
      721 tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag aaaaatgtct
      781 tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag cgtttcatag
      841 acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt ttgatcccaa
      901 aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct accaccttga
      961 ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc tatccttaat
     1021 ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct ctcccgccta
     1081 gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata aaaaaacagt
     1141 agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa
     1201 tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa aatatctaca
     1261 taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc actgcattga
     1321 ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct gattcttagc
     1381 gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa tttattgtaa
     1441 gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat gatttatata
     1501 tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat tcctttaaaa
     1561 aagattcttt tatataagtg aaaaaagctt ttttccacca attaaataaa tctctcatct
     1621 caatagttta tccattattt tatctttttt tgcgtatacc tactaaatat taccgtcttc
     1681 ttctcgttct tctactttgt tcgagagaat ctgtactaga aaaagtagtt gtatgtctac
     1741 cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta
     1801 cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca
     1861 aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc
     1921 tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat
     1981 tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt
     2041 ttcccatagt gaacacacac ctttttttat a
//
LOCUS       DACETN010000163         2056 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DACETN010000163 DACETN010000000
VERSION     DACETN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.170"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            493..714
                     /locus_tag="IXF98_002577"
     CDS             493..714
                     /locus_tag="IXF98_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556855.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IXF98_002578"
     CDS             715..972
                     /locus_tag="IXF98_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556856.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IXF98_002579"
     CDS             complement(1108..1710)
                     /locus_tag="IXF98_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1556857.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IXF98_002580"
     CDS             complement(1777..1932)
                     /locus_tag="IXF98_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1556858.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACETN010000164         2034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DACETN010000164 DACETN010000000
VERSION     DACETN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.171"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXF98_002581"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXF98_002581"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1556859.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IXF98_002582"
     CDS             complement(1431..1823)
                     /locus_tag="IXF98_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1556860.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACETN010000165         2000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DACETN010000165 DACETN010000000
VERSION     DACETN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.172"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            210..1028
                     /locus_tag="IXF98_002583"
     CDS             210..1028
                     /locus_tag="IXF98_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1556861.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1258..1488
                     /locus_tag="IXF98_002584"
     CDS             1258..1488
                     /locus_tag="IXF98_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1556862.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1711..1809)
                     /locus_tag="IXF98_002585"
                     /pseudo
     CDS             complement(1711..1809)
                     /locus_tag="IXF98_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          686 a          296 c          315 g          703 t
ORIGIN      
        1 gaaacaatca aaccttttga aataagatct aaaaacctct ttttatccat tatcctaaac
       61 gctccaattt tttattattt ttggataata actccaaaaa caatatagca tccgctttca
      121 taaaagtaaa gataaaaatg gagtatttag gagttttttt attattttgg agtataatgg
      181 agaaaagttg aaattgtaga ggagtttgta tgagttatat ctatttaatt aaagaaaatt
      241 atccattttt aacgaagtct gaaaaaaaag tcgctgattt tattttaaat tctgggaaaa
      301 ctattattta tagtacgatg agtgatatta agaaaaacac aaaagttggc gatgctacta
      361 ttattcgctt ttgtcaaaaa attggttttt ctggtttttc tgatttaaaa attgaaattg
      421 caaaggaaga ctttagtcaa aaaaaagaac atccgactag tgtaaagtac tatgatgaaa
      481 ttgctaatag tttaacagaa gctctctatt ccactattag gcttttagac gaagaaaaat
      541 tgaatgaagc cattaaaatg atcactcaat caaaaaacat atatatattt ggagtgggat
      601 ctagtggaaa tactagtctt gatttagaaa atatgtttct tcgcgtagga gttcaagcaa
      661 aagctgtact agaccctcat tttcaagccc aagtcgcttc tttattaaca gttaatgatt
      721 tagtaatcat tttttcttta tctgggaaaa cgaaagatac ttatgattca ttgaaaatag
      781 ctaaaaaaaa tggcgcaaaa attattgcta taactaacta tattcattca cctataggaa
      841 agtctgcaga cttagtttta caaacagcca ttgaagaatt tcttaatggt ggatctttag
      901 ctggaaaaat atcacaactt tatatttgtg atctattagt tcatggctat gaacaaaaca
      961 ataatattga ttcagttgaa ttaagagaaa aagttttgcg ctctattatt gataaaagta
     1021 ttgagtgaac tacccccact tagctaaacc taacggtttt taacgattga agtaggcaat
     1081 tcttgggatg agagcatact tgatagctta ttttttacta atagcggtgg ctcttattga
     1141 ccaaatatcc ctgtagttcc tacggttctt gattttatct aaagatattt ttatcgtgga
     1201 agctattgat caacttgaaa gaaaaacgtg tagctcatta tcactagttt atcgagtatg
     1261 gatgaagcaa tggatcatcc tctcttaaat aaatttatta aggatctgat ccttcaaatt
     1321 ttttccatga tcgatgagca ggaacctact gaatctaaac gacatcgggc acaaggaatt
     1381 attaagattg ctaagcgaat gttttctata aagggtgttc taagctatac agtgttaatt
     1441 caaaagattc ccaatcatct tgtttataag aacaacgttg aagattaaaa aagtgtttca
     1501 atgctaaaat tgcaaaatac ttcaatgtta tgagacaaac tgtctataag aatcaatttc
     1561 cacccatttc atcacttgtt aaaagtttat gatccttttg atttttatat taatttatct
     1621 gtatttaata cagattttat ttattagttc atctgcctat tgtattttat tttgttgcct
     1681 taccatctga agtaaatata ctcttaattg tcaaattaag ttagacagat catcaatact
     1741 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaagatt
     1801 atttctgcgc cagcattttc taatgtttga atttgtgttt tgtaatcttg gttgcttgtg
     1861 ctaattcttg aatagccgta aattgccaaa atttcatcac cctttctgga cataagtttc
     1921 tgacaccagt taaaatcaag tctatcagta acccatacac ccgtgaggac gggtgtattt
     1981 ttaatccccc tagaaggggt
//
LOCUS       DACETN010000166         1861 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DACETN010000166 DACETN010000000
VERSION     DACETN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.173"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(317..1609)
                     /locus_tag="IXF98_002586"
     CDS             complement(317..1609)
                     /locus_tag="IXF98_002586"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1556863.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRILTFSVGPDRTQTNILESVWAQI"
BASE COUNT          538 a          388 c          331 g          604 t
ORIGIN      
        1 agtggagagg ttgtgaaaaa gctgctctgc atcaagcagt aagaacaatc agataaaaat
       61 agcactccat atttttcatc tgattgggct tagtgccgta gatgcaagct tttgaacacc
      121 gtttgttcgg aaaaagggac tgtgacacgg tttttgtcac agtcccttat tctgaataaa
      181 cagtgttcaa gagacagcct atcggcaata aggctaaacg tctctttcac tacctcttca
      241 agttattatt atcgtataga taaacacttc gactcgctct ggtaagaacg tcctgattca
      301 gtcgtcgttt ttatccttag atttgcgccc agacgctttc taagatgttt gtttgtgttc
      361 tatcaggtcc gacagaaaat gttaagatac gtacaccaac aagctctgag atacgacgta
      421 catagttacg tgcgttttct ggcagttctg caagtgtttt gcatcctgta atgtcttctg
      481 accagcctgg caattcttca tagattggtt tgcagcggtt caactctttc aagcttgctg
      541 gatagtggta gattagttcg ccatctaatt cataagccgt acaaattttt actgtttcca
      601 atccacttaa tacatcgatc gagtttaaag ataggttggt aatacctgaa actcgtttag
      661 aatggcgcat aactacagaa tcaaaccagc ctacacgacg cggacgacca gttgtcgtac
      721 cgtattctcg tcctacttcg cggatttgtt ctcctgtttc atcaaataat tcagttggaa
      781 atggtccatc accgacacgt gacgtatagg ctttgcagac gccgacaact ttattgattt
      841 ttgaaggccc tacaccgctt ccgattgtta ccccaccggc tactgggtta gaagaagtaa
      901 caaaaggata agttccctgg tcgatatcca gcattacacc ttgtgcgcct tcaaacaaca
      961 cgcgtttgcc tgcatccaaa gcatcattca agataacgga agtatctgtg acgtattgtt
     1021 tgatttgttg tccgtattca taatattctt caaaaatatc gtcaaaatca agtggttctg
     1081 aatcaaacat ttttacaaat tgacggtttt tgtcatctag attcaaacga agacgttctt
     1141 caaaaatttc tttatctaat aagtctgcaa tacggatacc tacacgagct gctttatcca
     1201 tataggcagg tccgattcct ttaatcgttg taccaatctt attttcgccc ttagcatctt
     1261 cttgtaattg gtccaatttg atatgataag gcaagattac gtgcgcgcgg tcagaaatac
     1321 gcaaattact tgtttctaca cctttttcat gtaaataagc taattcttta accaatgatt
     1381 taggattgac tacaacgccg tttccgatca cactgatctt gtctttataa aaaatcccag
     1441 aaggaatcaa atgcagttta taagtcacac cgttaaactt aattgtgtgc cctgcattgt
     1501 cgccaccttg gtaccgtgca atcacttcag cattttcact gagaaagtct gtgatttttc
     1561 ctttcccttc atcgccccat tgcgtaccta ctactactac agatgacatg tgaacacctc
     1621 gttctatatt atttaatcct gaaccattgt atcaataata gatacatatt tcaaataaaa
     1681 tcgaatgatt tttattttta ttttataaaa acgcttaaaa aacgaacttt atctttttag
     1741 aatggttatt aaaaaagagg ttgtaacaga agtggtcagc ttcaagaaat aagaaggaat
     1801 gtacgaaaat tgcttctcaa atttttgtga aatttcgact tatttttcga agaagctact
     1861 t
//
LOCUS       DACETN010000167         1859 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DACETN010000167 DACETN010000000
VERSION     DACETN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.174"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IXF98_002587"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IXF98_002587"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1556864.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACETN010000168         1852 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DACETN010000168 DACETN010000000
VERSION     DACETN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.175"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(493..1785)
                     /locus_tag="IXF98_002588"
                     /pseudo
     CDS             complement(493..1785)
                     /locus_tag="IXF98_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          577 a          371 c          310 g          594 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tgtacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctca ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga tt
//
LOCUS       DACETN010000169         1829 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DACETN010000169 DACETN010000000
VERSION     DACETN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.176"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(430..1002)
                     /locus_tag="IXF98_002589"
     CDS             complement(430..1002)
                     /locus_tag="IXF98_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1556865.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1018..1623)
                     /locus_tag="IXF98_002590"
     CDS             complement(1018..1623)
                     /locus_tag="IXF98_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1556866.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          537 a          368 c          272 g          652 t
ORIGIN      
        1 taatatcaca ataggaatgc tgtttattat attagcagca gtgctgattt ttatgatagt
       61 tatttggttc ttaaatgaaa gatctaaaga caatcaacac cgtagggata tggagataaa
      121 atcaatagag tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac
      181 gtgacttacg ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata
      241 tatgatggta tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta
      301 cagttaccat acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac
      361 aacatgctaa tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag
      421 acttcttttt taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa
      481 accggttata gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg
      541 cctcttcttc ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt
      601 tcttgcaaac atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc
      661 tacactcaac aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag
      721 attcaaaacc ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc
      781 tctcgtgtta cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag
      841 accagccaac agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga
      901 aaaaatattc ttcttctcta cgccgttctc aagcaactgt tgaatttgtt cttttgtact
      961 ttgagaagcg gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta
     1021 caaatcatct atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt
     1081 catatataac tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat
     1141 ttccaaatcg tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa
     1201 gttccaatct acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt
     1261 acttcggcca tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat
     1321 aatctcttca aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata
     1381 cataactggt gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc
     1441 aaaatcgtaa atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc
     1501 aacttcgatt tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag
     1561 tttttcttca accttagcca actcaacttg attggttaat cccaatttat tctctaaaat
     1621 catgcgaaat ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata
     1681 tcgcgatttt gtttatgtgt caaaaactat taagtttctg accctcgata gatttttcaa
     1741 gtgtctctat tttgcacagt ctagttggtt ctgttgcaaa gttttaaata aagaataaaa
     1801 tcccttacgg tatctatgat ttaagctgg
//
LOCUS       DACETN010000170         1813 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DACETN010000170 DACETN010000000
VERSION     DACETN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.177"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IXF98_002591"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IXF98_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1556867.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IXF98_002592"
     CDS             complement(1305..1709)
                     /locus_tag="IXF98_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1556868.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          539 a          317 c          289 g          668 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tca
//
LOCUS       DACETN010000171         1812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DACETN010000171 DACETN010000000
VERSION     DACETN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.178"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(128..604)
                     /locus_tag="IXF98_002593"
     CDS             complement(128..604)
                     /locus_tag="IXF98_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1556869.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(617..>1812)
                     /locus_tag="IXF98_002594"
     CDS             complement(617..>1812)
                     /locus_tag="IXF98_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1556870.1"
                     /translation="GFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLG
                     FFGGSRFIPIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRL
                     TGAVGLHHTIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFA
                     GRFATMMFGLPAACYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAP
                     WLYVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIA
                     WAFIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENI
                     ESVEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQI
                     LGKDN"
BASE COUNT          652 a          343 c          255 g          562 t
ORIGIN      
        1 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
       61 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      121 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      181 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      241 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      301 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      361 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      421 atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg
      481 caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa
      541 tgatttttcc ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa
      601 acatattaaa aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata
      661 agtcagcttt ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg
      721 caccagtgtc ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca
      781 aacgagttgc acaagcttca acagactcaa tattttcttt tccaccaaga gcttcaataa
      841 tgataagaga ctcttcatgt aatgatgtat ttttgtttat tactttttca gaatgaactt
      901 ctgattcttc ttccattcca ggaatagcta ccttaaattt tttgataaag aatgtaaaaa
      961 caaagaaata tataaatgcc catgcaatac caaaaggaat taattttaac caattagttt
     1021 tgtcattccc ttgaagtaca ccgaataata aaaagtcgat caaaccacct gaaaaggaat
     1081 tccctacacg aatatttaag atatcagcaa aataaaaaga taatccatct aagaatgcat
     1141 gaatcacata aagccaagga gcaacaaata aaaacatata ttcaactggt tcagtaattc
     1201 cagttaaaaa tgatgtaagg gccccgctaa aataaagccc accattcttt ttacgatttt
     1261 tcttaggaat tgcacgatac attgcataac atgctgctgg taatccaaac atcattgttg
     1321 caaatcttcc tgcaaaaaat ctagtaccat aagtaaacag accagtgtgg tttggatcag
     1381 ctagttgggc aaagaaaata ttttgggctc cagcaattgt tgatccagct acagtttcgg
     1441 taccacctaa agatgtatac cagaacaaag ggtaaatggt gtggtgtagt ccaacagcac
     1501 ctgttaaacg caataaaaaa ccatataaaa aagttcctaa actacccatt tgggcaattt
     1561 gttcaccagc aactactaaa ccattttgaa ttggtggcca aattaaataa aagaaagaac
     1621 caataatgat tgcagctaaa ctagaaacaa tcggaataaa tcttgatcct ccaaaaaaac
     1681 ctaagaattg tggtaattca atttttctat aacgattgtg taaaaatgca acagtaccac
     1741 caacaacaat tgatcccact acacctgtat ctagtgttgc atcttttgct gaaaaaagtt
     1801 caagaaaccc ac
//
LOCUS       DACETN010000172         1778 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DACETN010000172 DACETN010000000
VERSION     DACETN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.179"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            1..72
                     /locus_tag="IXF98_002595"
     CDS             1..72
                     /locus_tag="IXF98_002595"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1556871.1"
                     /translation="MCSLLVGLLSALFSYWLNNRNKK"
     gene            complement(374..619)
                     /locus_tag="IXF98_002596"
     CDS             complement(374..619)
                     /locus_tag="IXF98_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556872.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            965..1249
                     /locus_tag="IXF98_002597"
     CDS             965..1249
                     /locus_tag="IXF98_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556873.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1252..1668
                     /locus_tag="IXF98_002598"
     CDS             1252..1668
                     /locus_tag="IXF98_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556874.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          614 a          324 c          267 g          573 t
ORIGIN      
        1 atttgctccc ttcttgttgg gcttttaagc gctttgttca gttactggtt gaataaccga
       61 aacaagaagt aaaacttaat taaggcgact cagcccacaa aaaaagcccc agagaatccg
      121 acctctctgg ggctttcgtg tgtttcgtgt tcacttattt gcaatcaaat tataacagaa
      181 ataaatattt atttcaaaaa tatcctacaa aagtctaaaa tccgtaatta tttcttatat
      241 gcaacattta caaataaagt cgctctgaga gctcgtattt ttcgatttaa gacgttttat
      301 ttagtttttg gtgtatttca ttggtccaag ctaaaaaact cttagaagcg attttagagc
      361 catttaaacc cacttaatta tattctccat gaaattcgct ccatgcgtca aaagctgcgt
      421 ctgaaattcc gccgtcttcg ttcactagtt gcattaattt ttccaacgat cgttttcttt
      481 cgggtgtcat atttttatat tccaacaatt tttcgtctgt taaatcgaac ttgattgctt
      541 gaattgcttg ctctaatttt tctaagtttt gaaaatattc gtaaggaaat ttttccattt
      601 tttctttctc cagtctcaca atttttctag aaataaaatc acttctttgc cgtctatact
      661 ctcaaaaaaa atgtcgtttg aaaaactaaa taaatttttg atcgtgcatt tttaaatttg
      721 gttatcgatt acacgtgcag cactcccccc ccgaatttta aagagcataa ggaaacgtgt
      781 acccactttc taaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa
      841 tttgatatca ctttttcgcc atcgtgggta gcttatgtat agcattttgg caaaaaacag
      901 ctatacaaat atagtatttg tatggtatac ataaattata cataattgca aaaggagatt
      961 ttttatggac attttagttc ggaatattga tccaaagttt gttgcaacaa tagacaaacg
     1021 ttgccaagaa ctcacgaata aaacaggaga aaaatggagt cgaaataaat atctaaaagt
     1081 tttaatcgaa agtgattttg accgtcaact tacgacttac aaaaaagacc aattcgattt
     1141 attgctcgat aaatttattg ctatccaaac agaaaatact caaatattaa gcgagtatgt
     1201 ctacacaaat aatcaaatca ttgaaatgct tatcggagga atgatataac catgagaaaa
     1261 acttctgtcc aaaaacatgt tactttgtac cctagtcatg ttgacgtttt agattccatc
     1321 atcgaaaaaa atccagatat caataactac tcggaagcca ttcgcttttt atgtatgagt
     1381 tatgaacccg ataccaacac gaccactacc agcaatcagg caaaactaaa tgctatggga
     1441 aaagaaattt ccatcatgac tgaaatcatc gcagaatttg ctgatttata tctaaaagat
     1501 acaggcgttc ttattggcac aaattctaaa gtatatcaag aagtgaaaga aaaaatcgaa
     1561 gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc atcaaaaaga aaccagtcaa
     1621 acagtcactt ctagaaattt cgacccaaca aattttctaa aacgataaaa aacagatagg
     1681 agttattcct atctgttttt tatttagaag ccccatacaa tatgctagaa tgtactagca
     1741 atcttagtcg gttgtaaggg acttgtctac ttgcttaa
//
LOCUS       DACETN010000173         1747 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DACETN010000173 DACETN010000000
VERSION     DACETN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.180"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            378..1628
                     /locus_tag="IXF98_002599"
     CDS             378..1628
                     /locus_tag="IXF98_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1556875.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          334 c          308 g          527 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catcttg
//
LOCUS       DACETN010000174         1654 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DACETN010000174 DACETN010000000
VERSION     DACETN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.181"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            138..1604
                     /locus_tag="IXF98_002600"
     CDS             138..1604
                     /locus_tag="IXF98_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1556876.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          558 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctgt tttt
//
LOCUS       DACETN010000175         1629 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DACETN010000175 DACETN010000000
VERSION     DACETN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.182"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            287..694
                     /locus_tag="IXF98_002601"
     CDS             287..694
                     /locus_tag="IXF98_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556877.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IXF98_002602"
     CDS             708..1109
                     /locus_tag="IXF98_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556878.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IXF98_002603"
     CDS             1111..1482
                     /locus_tag="IXF98_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556879.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          209 c          283 g          525 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtataca
//
LOCUS       DACETN010000176         1608 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DACETN010000176 DACETN010000000
VERSION     DACETN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.183"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXF98_002604"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXF98_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1556880.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            750..1608
                     /locus_tag="IXF98_002605"
                     /pseudo
     CDS             750..1608
                     /locus_tag="IXF98_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          522 a          307 c          305 g          474 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt
      841 ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag
      901 agggtctagt aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga
      961 tagagttata tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga
     1021 acaaccctaa tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac
     1081 tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga
     1141 aaaaatctac aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac
     1201 gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac
     1261 aggaagccca acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc
     1321 gtttaaatta gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg
     1381 gcaaaaacga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt
     1441 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
     1501 tttctctatc aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac
     1561 gacatacaca cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DACETN010000177         1594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DACETN010000177 DACETN010000000
VERSION     DACETN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.184"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1485
                     /locus_tag="IXF98_002606"
     CDS             <1..1485
                     /locus_tag="IXF98_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1556881.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACETN010000178         1582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DACETN010000178 DACETN010000000
VERSION     DACETN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.185"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            292..1479
                     /locus_tag="IXF98_002607"
     CDS             292..1479
                     /locus_tag="IXF98_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1556882.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          315 g          504 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta ct
//
LOCUS       DACETN010000179         1539 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DACETN010000179 DACETN010000000
VERSION     DACETN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.186"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..255)
                     /locus_tag="IXF98_002608"
     CDS             complement(<1..255)
                     /locus_tag="IXF98_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1556883.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKN"
     gene            complement(252..905)
                     /locus_tag="IXF98_002609"
     CDS             complement(252..905)
                     /locus_tag="IXF98_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1556884.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1193..>1539
                     /locus_tag="IXF98_002610"
     CDS             1193..>1539
                     /locus_tag="IXF98_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556885.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          496 a          267 c          184 g          592 t
ORIGIN      
        1 atttttttct tttatgaata cttttatttc actactcttg gaataaaact ccaaagtttg
       61 ttctatatat tttatttctt ttccattcat attactagaa atttcatttg ccatattatg
      121 aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg
      181 aactaatata attattattc caagaacaga aaatatttgt atgaacattt ttggaaatat
      241 ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt agttatacag
      301 tctaattgta atttttttct aagttctttt atatatacat ctattactcg atcataagga
      361 gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa cacttgtcca
      421 ttattatcca ataaacattt taatacttct agttcttttg catttacatc tatttcttca
      481 ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata ttcaaacttc
      541 tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa gaccggcaat
      601 gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat tttatattct
      661 tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct aatttctttc
      721 aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa atttatatcg
      781 ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt atatccaaat
      841 tctgaaagat attcacttat tccctctctt atcatattat catcttcaac agttaatatt
      901 ttcatatata cctccactta agcctataca ctagatatta tacagcatat tatggctcta
      961 taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt ttaataaatg
     1021 caaacagaca ccttgaaatc ctttagaata aagggttcta taatattttg tgttgttgga
     1081 aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa agagactcac
     1141 ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct ctatggataa
     1201 tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg aagaatggct
     1261 gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat tggatgactc
     1321 accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt cctatacgac
     1381 acgtactcag atattgaaag tcaaagaaaa gttgactatt ttagaattaa aacgtacccg
     1441 ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag ttgatgaaca
     1501 tcatcaacta acaaaggaac tgaagcaggc gattctcat
//
LOCUS       DACETN010000180         1458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DACETN010000180 DACETN010000000
VERSION     DACETN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.187"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(100..1119)
                     /locus_tag="IXF98_002611"
     CDS             complement(100..1119)
                     /locus_tag="IXF98_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1556886.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="IXF98_002612"
     CDS             complement(1130..1336)
                     /locus_tag="IXF98_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1556887.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          403 a          304 c          246 g          505 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa ttttatct
//
LOCUS       DACETN010000181         1456 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DACETN010000181 DACETN010000000
VERSION     DACETN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.188"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            108..1040
                     /locus_tag="IXF98_002613"
     CDS             108..1040
                     /locus_tag="IXF98_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1556888.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IXF98_002614"
     CDS             1112..>1456
                     /locus_tag="IXF98_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556889.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACETN010000182         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DACETN010000182 DACETN010000000
VERSION     DACETN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.189"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(150..1337)
                     /locus_tag="IXF98_002615"
     CDS             complement(150..1337)
                     /locus_tag="IXF98_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1556890.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACETN010000183         1392 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DACETN010000183 DACETN010000000
VERSION     DACETN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.190"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(41..125)
                     /locus_tag="IXF98_002616"
     tRNA            complement(41..125)
                     /locus_tag="IXF98_002616"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Leu,seq:caa)
     gene            complement(143..214)
                     /locus_tag="IXF98_002617"
     tRNA            complement(143..214)
                     /locus_tag="IXF98_002617"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Gln,seq:ttg)
     gene            complement(224..296)
                     /locus_tag="IXF98_002618"
     tRNA            complement(224..296)
                     /locus_tag="IXF98_002618"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:His,seq:gtg)
     gene            complement(358..442)
                     /locus_tag="IXF98_002619"
     tRNA            complement(358..442)
                     /locus_tag="IXF98_002619"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Leu,seq:caa)
     gene            complement(463..533)
                     /locus_tag="IXF98_002620"
     tRNA            complement(463..533)
                     /locus_tag="IXF98_002620"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Cys,seq:gca)
     gene            complement(579..650)
                     /locus_tag="IXF98_002621"
     tRNA            complement(579..650)
                     /locus_tag="IXF98_002621"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Gln,seq:ttg)
     gene            complement(662..734)
                     /locus_tag="IXF98_002622"
     tRNA            complement(662..734)
                     /locus_tag="IXF98_002622"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:His,seq:gtg)
     gene            complement(737..810)
                     /locus_tag="IXF98_002623"
     tRNA            complement(737..810)
                     /locus_tag="IXF98_002623"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Trp,seq:cca)
     gene            complement(817..897)
                     /locus_tag="IXF98_002624"
     tRNA            complement(817..897)
                     /locus_tag="IXF98_002624"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(861..863),aa:Tyr,seq:gta)
     gene            complement(899..974)
                     /locus_tag="IXF98_002625"
     tRNA            complement(899..974)
                     /locus_tag="IXF98_002625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Phe,seq:gaa)
     gene            complement(1010..1082)
                     /locus_tag="IXF98_002626"
     tRNA            complement(1010..1082)
                     /locus_tag="IXF98_002626"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1047..1049),aa:Asp,seq:gtc)
     gene            complement(1092..1164)
                     /locus_tag="IXF98_002627"
     tRNA            complement(1092..1164)
                     /locus_tag="IXF98_002627"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1129..1131),aa:Val,seq:tac)
     gene            complement(1180..1251)
                     /locus_tag="IXF98_002628"
     tRNA            complement(1180..1251)
                     /locus_tag="IXF98_002628"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1216..1218),aa:Glu,seq:ttc)
     gene            complement(1266..1355)
                     /locus_tag="IXF98_002629"
     tRNA            complement(1266..1355)
                     /locus_tag="IXF98_002629"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Ser,seq:gga)
BASE COUNT          393 a          365 c          320 g          314 t
ORIGIN      
        1 atagcaactc tctataagaa agttgctata ttgataatta taccggcggc cggggtcgaa
       61 ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa ttccgccacg
      121 ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca tgagggagtc
      181 aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat gatgggaatc
      241 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatc
      301 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
      361 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      421 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      481 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      541 tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      601 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      661 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      721 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      781 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      841 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      901 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      961 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
     1021 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
     1081 ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
     1141 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
     1201 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
     1261 tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct acatgatttc
     1321 caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct caatactctt
     1381 tctcaaaatg ag
//
LOCUS       DACETN010000184         1368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DACETN010000184 DACETN010000000
VERSION     DACETN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.191"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            82..>1368
                     /locus_tag="IXF98_002630"
     CDS             82..>1368
                     /locus_tag="IXF98_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1556891.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACETN010000185         1355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DACETN010000185 DACETN010000000
VERSION     DACETN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.192"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>1355
                     /locus_tag="IXF98_002631"
     rRNA            <1..>1355
                     /locus_tag="IXF98_002631"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACETN010000186         1348 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DACETN010000186 DACETN010000000
VERSION     DACETN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.193"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..330
                     /locus_tag="IXF98_002632"
     CDS             <1..330
                     /locus_tag="IXF98_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1556892.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IXF98_002633"
     CDS             complement(844..1011)
                     /locus_tag="IXF98_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1556893.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..>1348)
                     /locus_tag="IXF98_002634"
     CDS             complement(1099..>1348)
                     /locus_tag="IXF98_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HAQ1556894.1"
                     /translation="KYCANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVC
                     LTELKGYQVTNIKSAFQPRRPNNRIDKVRHMFNKINLH"
BASE COUNT          440 a          204 c          227 g          477 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttg
//
LOCUS       DACETN010000187         1247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DACETN010000187 DACETN010000000
VERSION     DACETN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.194"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            200..742
                     /locus_tag="IXF98_002635"
     CDS             200..742
                     /locus_tag="IXF98_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556895.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1247
                     /locus_tag="IXF98_002636"
     CDS             832..>1247
                     /locus_tag="IXF98_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1556896.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DACETN010000188         1244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DACETN010000188 DACETN010000000
VERSION     DACETN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.195"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            70..>1244
                     /locus_tag="IXF98_002637"
     CDS             70..>1244
                     /locus_tag="IXF98_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1556897.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT"
BASE COUNT          461 a          233 c          206 g          344 t
ORIGIN      
        1 acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag
       61 gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat
      121 ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa
      181 ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg
      241 agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata
      301 cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac
      361 gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt
      421 gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg
      481 actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat
      541 gtcgctattt ttatggacgc tacttacatt ccactaaaac gtcaaaccgt atccaaagaa
      601 gcgatttata ttgccattgg tatacgagaa gacggcacta aagaagtact gagttatgcg
      661 attgctccaa ctgaatcaac atacgtttgg aatgagctgc tacaggatat taactccaga
      721 ggagttcaag aagtcttgct ttttattacg gacggcttaa aaggcatgaa agatactatc
      781 catcaaattt atcctaaagc aaaatatcag cattgttgta tccatgtatc tcgtaacatc
      841 gctcataaag tacgtgtcaa agaccgaaaa gaaatctgtg atgactttaa ggctgtttat
      901 caagctaact caaaagaaga agcgaatacc ttcttatccg gcatgattga gaaatggaag
      961 aaaaactatc ctaaagtgac gcagtcactc atagaaaacc aagacttatt aactttttat
     1021 gattttccac ctagcattcg tagaaccatt tactcaacca atctaatcga gtctttcaat
     1081 aagcaaatta aaagatacag ccgtagaaaa gagcagtttc aaaatgaaga atcactagaa
     1141 cgctttctag tcagcatttt tgatacatac aatcaaaaat ttctaaacag aagccataaa
     1201 ggttttcaac aggtaaccga tacattagtt tcaatgttta ctga
//
LOCUS       DACETN010000189         1239 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DACETN010000189 DACETN010000000
VERSION     DACETN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.196"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            825..1037
                     /locus_tag="IXF98_002638"
     CDS             825..1037
                     /locus_tag="IXF98_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556898.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          458 a          187 c          206 g          388 t
ORIGIN      
        1 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
       61 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
      121 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
      181 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
      241 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
      301 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      361 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      421 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      481 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      541 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      601 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      661 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      721 aataaaaaat aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa
      781 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      841 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      901 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
      961 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg
     1021 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
     1081 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
     1141 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
     1201 atcaagagaa agttaagcac acattatgag cgtcaaaca
//
LOCUS       DACETN010000190         1215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DACETN010000190 DACETN010000000
VERSION     DACETN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.198"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            414..947
                     /locus_tag="IXF98_002639"
     CDS             414..947
                     /locus_tag="IXF98_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1556899.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACETN010000191         1187 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DACETN010000191 DACETN010000000
VERSION     DACETN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.199"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            141..398
                     /locus_tag="IXF98_002640"
     CDS             141..398
                     /locus_tag="IXF98_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556900.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IXF98_002641"
     CDS             complement(630..1043)
                     /locus_tag="IXF98_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1556901.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          150 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatc
//
LOCUS       DACETN010000192         1185 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DACETN010000192 DACETN010000000
VERSION     DACETN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.200"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1052
                     /locus_tag="IXF98_002642"
     CDS             <1..1052
                     /locus_tag="IXF98_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556902.1"
                     /translation="NNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFIGQKV
                     YPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYKEY
                     AKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKAKD
                     NYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNLE
                     NIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSII
                     IYKKNSFKYFKENIL"
BASE COUNT          488 a          139 c          127 g          431 t
ORIGIN      
        1 aaaataacta cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa
       61 cttttattat cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta
      121 ttggacaaaa agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa
      181 tatgtgttga tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg
      241 acttaaattt aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata
      301 aagaatatgc aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg
      361 actctcctcc tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa
      421 tatttgttaa taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag
      481 ataactatac aataactgat cccatagtta gagttattaa tccagaaata acagaatcat
      541 tattttgggg aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt
      601 caaaccaaaa cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata
      661 tcataaattt ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag
      721 taaataaatt aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta
      781 attttcaaat gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa
      841 tatttggata ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag
      901 agttatttgc tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta
      961 taatttttat gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata
     1021 gttttaaata ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc
     1081 aattaataaa aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat
     1141 tatagtaaca ggaaaaagtg gtagtggaaa aactactctg ttaaa
//
LOCUS       DACETN010000193         1137 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DACETN010000193 DACETN010000000
VERSION     DACETN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.201"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..118)
                     /locus_tag="IXF98_002643"
     CDS             complement(<1..118)
                     /locus_tag="IXF98_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HAQ1556903.1"
                     /translation="MSRFFKFGKLHVTKGNGDKLLDILLTASKKLKRSLAPTG"
     gene            complement(123..860)
                     /gene="erm(B)"
                     /locus_tag="IXF98_002644"
     CDS             complement(123..860)
                     /gene="erm(B)"
                     /locus_tag="IXF98_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1556904.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(985..1068)
                     /locus_tag="IXF98_002645"
     CDS             complement(985..1068)
                     /locus_tag="IXF98_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1556905.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT          342 a          185 c          184 g          426 t
ORIGIN      
        1 tccccgtagg cgctagggac ctctttagct tcttggaagc tgtcagtagt atatctaata
       61 atttatctcc attcccttta gtaacgtgta actttccaaa tttaaaaaag cgactcatag
      121 aattatttcc tcccgttaaa taatagataa ctattaaaaa tagacaatac ttgctcataa
      181 gtaacggtac ttaaattgtt tactttggcg tgtttcattg cttgatgaaa ctgattttta
      241 gtaaacagtt gacgatattc tcgattgacc cattttgaaa caaagtacgt atatagcttc
      301 caatatttat ctggaacatc tgtggtatgg cgggtaagtt ttattaagac actgtttact
      361 tttggtttag gatgaaagca ttccgctggc agcttaagca attgctgaat cgagacttga
      421 gtgtgcaaga gcaaccctag tgttcggtga atatccaagg tacgcttgta gaatccttct
      481 tcaacaatca gatagatgtc agacgcatgg ctttcaaaaa ccactttttt aataatttgt
      541 gtgcttaaat ggtaaggaat actcccaaca attttatacc tctgtttgtt agggaattga
      601 aactgtagaa tatcttggtg aattaaagtg acacgaatgt tcagttttaa tttttctgac
      661 gataagttga atagatgact gtctaattca atagacgtta cctgtttact tattttagcc
      721 agtttcgtcg ttaaatgccc tttacctgtt ccaatttcgt aaacggtatc ggtttctttt
      781 aaattcaatt gttttattat ttggttgagt actttttcac tcgttaaaaa gttttgagaa
      841 tattttatat ttttgttcat gtaatcactc ctgaagttat tacatctgta aataaataca
      901 gaagttaaac gatttgtttg taattttagt tatctgttta aaaagtcata agattagtca
      961 ctggtaggaa ttaatctaac gtatttattt atctgcgtaa tcactgtttt tagtctgttt
     1021 caaaacagta gatgttttat ctacattacg catttggaat accaacatga cgaatccctc
     1081 cttcttaatt acaaattttt agcatctaat ttaacttcaa ttcctattat acacaaa
//
LOCUS       DACETN010000194         1123 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DACETN010000194 DACETN010000000
VERSION     DACETN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.202"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(206..760)
                     /locus_tag="IXF98_002646"
     CDS             complement(206..760)
                     /locus_tag="IXF98_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1556906.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          362 a          217 c          130 g          414 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatacttcc gaaaattcag ttatcttcat taatctcacc ttctaagttt tcattataca
      181 caaaaaatat atcctaatat ctcaattagc ctcccaattt aaaaccacgt aacgatgccc
      241 ctatttcatc ttcagttata ccaatgtacc tttttgtaat tgcttgcgag ctatgattaa
      301 aaataaacat taaggtagca atgtctctag ttttcttgta gtagtgatac ccaaaagttt
      361 ttcttaatgt atgtgttcct atatcatctc ttcctaattt ttctgctaca tctgcatata
      421 tctgatagac acgatccctt gttatatgtc gaccttgttg ccgacttgga aataaccaat
      481 cattttcttc tttaccttca gtatataata aaatctcttc tctcaaatta tctataaaaa
      541 tcggttttcg cttacctgtt ttatgttcaa caataatagg cttactttta cctcgtatgt
      601 ctccaacttt taatcctata atatctgaca tacgtaaccc attattgatt ccaaacaaaa
      661 ataaaaaact atctctttct ggattacttg ttcttcttag ccaaaactga aaatcttgta
      721 tctcttcatt tgtccttaac ggttgtacat tatagctcac tattgtttcc cttcttaaaa
      781 atattctgag ttcatgatta tatatatatt ttaccataat catgaactta tatatatact
      841 ctgttaaaag tgaagaaaat caaggggttt agtttatgat tttatatcat ttcagtaact
      901 cagaatattc cgactttttt gtacaaaaaa attaacctta cttatatgaa gattctaagc
      961 tcttatttaa attttagacg ggcagttatc cgtagaagtt cttagaatag ctaatttaca
     1021 taattaaatt atatgaaatt taagtttgaa caatatacta agaactcccc ttactataaa
     1081 gtgaaataaa ttagcatatg aataaccgtg ctaccaaact ttt
//
LOCUS       DACETN010000195         1091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DACETN010000195 DACETN010000000
VERSION     DACETN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.203"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            6..980
                     /locus_tag="IXF98_002647"
     CDS             6..980
                     /locus_tag="IXF98_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1556907.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACETN010000196         1082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DACETN010000196 DACETN010000000
VERSION     DACETN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.204"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            700..>1082
                     /locus_tag="IXF98_002648"
     CDS             700..>1082
                     /locus_tag="IXF98_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1556908.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACETN010000197         1069 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DACETN010000197 DACETN010000000
VERSION     DACETN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.205"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..90
                     /locus_tag="IXF98_002649"
     CDS             <1..90
                     /locus_tag="IXF98_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1556909.1"
                     /translation="RRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          398 a          142 c          156 g          373 t
ORIGIN      
        1 cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag
       61 accattcgag gaatatataa aaagaactga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaaa tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga acccattagc aatatataaa agaaggtatc
      241 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      301 taagaactaa ataggaaaac gtagacgtaa ttttctacgt tttccttttc atatatatta
      361 aatattacgg aaaattatat aaactaattt agtgttttgt ttgtaattcc taattttgct
      421 ccacttttag ataattttta ggtctattta actcattaga cattctatgc tcatttttct
      481 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      541 gtacttttat tacgatacta gtatgatata tatctacagc aaacagttaa aatcttccta
      601 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      661 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      721 cttatactta ttgcgcaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      781 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
      841 ttagtataaa ttaacatact actatatttt aggtttcata ttatataaat taataatata
      901 ctaccatttg ttggtagtat aatttaaagg taataagtta ttcttttatt actatgtttg
      961 ggttttatag caaagaaagt ttaataagag tttggaccta gcaaacaata ttaatgaggt
     1021 cattaaatct tattatcttt agtgatataa tgaaatatct acacgacaa
//
LOCUS       DACETN010000198         1043 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DACETN010000198 DACETN010000000
VERSION     DACETN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.206"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(170..>1043)
                     /gene="menA"
                     /locus_tag="IXF98_002650"
     CDS             complement(170..>1043)
                     /gene="menA"
                     /locus_tag="IXF98_002650"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1556910.1"
                     /translation="FSIAFFHEIHWLNTFLFFIGMLVFDLATTAINNYMDYEKAHSEV
                     YKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVILTINTGWLLLLMGFICCFIGVFY
                     TFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFGSDVYDLTVNFSSGQFDLVGNLW
                     AIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHRYTLVFYIGRPAGTVLFQLLMYA
                     CYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFKQTLPQPISFSYSIKNMVLFNAS
                     YVLGLLLTIIMQAI"
BASE COUNT          381 a          210 c          178 g          274 t
ORIGIN      
        1 cttaatactc gaaactaagc gactttttca caacctcctg ataaacggtg ttcaaaagct
       61 tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt ttatttggct
      121 gttcttatta cttgatgcag ggcagttttt tcacaacctc ttcatgctgt taaatggctt
      181 gcataatgat tgttagtaat agccccaata cataactggc attgaataag accatatttt
      241 tgatggaata gctgaaactg ataggctggg gtaacgtttg cttgaattga tccaaatgct
      301 tcttcacaat tggcaaggtc aagaaggtga ccaagatcgg ccattcatag ataccgctca
      361 acaaccccat taaaatcacg aaatagcaag catacatcaa tagctggaat aagactgttc
      421 ccgcaggacg tccgatataa aaaactagtg tatagcgatg attttcaata tcacggtcaa
      481 gatcccgcaa attattagcc aacatgatat tggcaatcgt aaacaccaag ggtaaggaag
      541 ctaatacgat tgctgctata gcccaaagat ttcctaccaa atcaaattgt ccagatgaaa
      601 agttgactgt cagatcatag acatctgaac cgaatgtatt gacataaacg accatggcaa
      661 aaattccaag gcccatagtc accccactga aaatttcccc taaaggcatt cgagataaag
      721 gaatcggtcc gaatgtatag aatactccaa taaaacaaca gataaatccc atcaaaagta
      781 aaagccaacc cgtattgatc gttagaataa cgccgataac aaaaacaaat gcaatcatag
      841 ctaaaatcat gtttcgaatc aatacaggag atagatttct gcggccaatg acattttctt
      901 catacttata tacttcagaa tgagcctttt cataatccat gtaattattg atagcggtag
      961 tcgctaaatc aaaaactagc attccaataa aaaataaaaa agtgtttagc caatgtatct
     1021 catgaaaaaa agcgatagag aat
//
LOCUS       DACETN010000199         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DACETN010000199 DACETN010000000
VERSION     DACETN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.207"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>1035)
                     /locus_tag="IXF98_002651"
     rRNA            complement(<1..>1035)
                     /locus_tag="IXF98_002651"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACETN010000200         1004 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DACETN010000200 DACETN010000000
VERSION     DACETN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.208"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            272..973
                     /locus_tag="IXF98_002652"
     CDS             272..973
                     /locus_tag="IXF98_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1556911.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          364 a          165 c          156 g          319 t
ORIGIN      
        1 tttattcatt gaaaaaattt ttttgaataa attaaaaaaa taaattatat tatcgttgtt
       61 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
      121 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
      181 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
      241 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
      301 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      361 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      421 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      481 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      541 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      601 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      661 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      721 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      781 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      841 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      901 cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      961 tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataa
//
LOCUS       DACETN010000201          998 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DACETN010000201 DACETN010000000
VERSION     DACETN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.209"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            186..383
                     /locus_tag="IXF98_002653"
     CDS             186..383
                     /locus_tag="IXF98_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556912.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            489..971
                     /locus_tag="IXF98_002654"
     CDS             489..971
                     /locus_tag="IXF98_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556913.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
BASE COUNT          329 a          137 c          212 g          320 t
ORIGIN      
        1 tgttttaatt ttttgaaaaa aattcttcta aatacaacag ctgttttaat tgtgaaaacg
       61 aaaacagtac gctataatgc ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag
      121 ttaaatctcc aaagaaatag cagtggtaat gtggagaaaa gttccagcag tttaggagga
      181 ttttgatgac ggaagaaatg attaatttag gagaacaata tgcatgcaaa ccaattggtt
      241 ttacaaaaac ggtcattggt gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag
      301 ttgcccaatg tgctgcagag gaccaagaat tattagatga aaaagcaagc atggttgttg
      361 caaaatatga tacatttgaa taaagaaaac aattggacgt gcggcgtgct gcaagtccaa
      421 tttctttgag aaggtaaatc agtattcttt tcctattttc tggagaagaa aaagcgaggc
      481 gagttgtcat ggtaacattc tttacggttc tattgttttt attcgttgtt gtgatgtttt
      541 tatttctttg gctggttcga aaagaaatta tttatcggac tgtgcgaaat cgatgggtct
      601 acttggtgat acccttctta gtgtcactag tcatttggta tacactcatc agtcatccta
      661 cagcagatga attggcaaaa gggatcttgt cagcaatgat ttttattagt tttttgttgg
      721 acagtagagg aatcacagaa gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc
      781 ctttttctga gatcaataaa attgtattat atcagccaaa aggttctaaa atcgtaaaaa
      841 tgaatttttt ccgaaacgga tggcgaggac cgatgctgaa attttctgca tcattagaag
      901 aattagttcc atttttgtcc caacgcttaa acgatgaggc agagattgat attatgattg
      961 atccagaata aaaacagagg ttgcgacaag atgtttgt
//
LOCUS       DACETN010000202          904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DACETN010000202 DACETN010000000
VERSION     DACETN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.210"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..868
                     /locus_tag="IXF98_002655"
     CDS             <1..868
                     /locus_tag="IXF98_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1556914.1"
                     /translation="ILFSAIISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSND
                     EVGELKQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKII
                     LENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLE
                     DILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGI
                     VNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQD
                     EEFFIFKIKIFS"
BASE COUNT          403 a           62 c          123 g          316 t
ORIGIN      
        1 aattttattt tctgctatta tttctttaat ttatgcaaaa tcaataaaaa ataatataca
       61 agaaataaaa attgttactg ataaaatgat gaaacttgat aaaaaaatga gtttaaaagt
      121 tagctctaat gatgaagttg gagaattaaa acaacaaatt aatgatttgt attctacttt
      181 attaagaaca attgacgatt tggaatttaa aaataaagaa attttaaaat tagaaaagtt
      241 aaaatatgac ttttttaaag gtgcatccca tgaacttaaa acccctcttg ctagtcttaa
      301 aataatactg gaaaacatga aatacaatat tggaaaatat aaagaaagag atatttatat
      361 taatgaatgt attgaaatcg ttgatagttt aacaaaaaat atttctcaaa tattatcagt
      421 tcattctata gaaaatttga ataatgatga agaatatttg aaaataaatg atacattaga
      481 agacatatta aaaaagtatg aaatattagc aaatcaaaag aaaataactg tcaataatta
      541 tatattagac gagaatgttt atataggaaa aacagcttta aagattattc tatctaattt
      601 gattagtaat gcggtaaaat atactgatgt aaatggggta attaatattg ggatagttaa
      661 tgattggtta tatatagaga attcatacgg taacaataaa atttcgaata tggataaaat
      721 atttgatgtt aaatttgatt taaataagga aaatagtaat ggattaggtt tatatattgt
      781 aagtaatatt ctgaataatt acaatataga atataaagta ttgcaggatg aagaattttt
      841 tatctttaaa ataaaaatat tttcttaatt aagatgtaat tttgcaatag ttattgaaaa
      901 atag
//
LOCUS       DACETN010000203          895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DACETN010000203 DACETN010000000
VERSION     DACETN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.211"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..618
                     /locus_tag="IXF98_002656"
     CDS             <1..618
                     /locus_tag="IXF98_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1556915.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..>895
                     /locus_tag="IXF98_002657"
     CDS             632..>895
                     /locus_tag="IXF98_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1556916.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAG"
BASE COUNT          313 a          116 c          194 g          272 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggt
//
LOCUS       DACETN010000204          860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DACETN010000204 DACETN010000000
VERSION     DACETN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.212"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..856
                     /locus_tag="IXF98_002658"
     CDS             <1..856
                     /locus_tag="IXF98_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1556917.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACETN010000205          843 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DACETN010000205 DACETN010000000
VERSION     DACETN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.213"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            212..808
                     /locus_tag="IXF98_002659"
     CDS             212..808
                     /locus_tag="IXF98_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1556918.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          309 a          155 c          138 g          241 t
ORIGIN      
        1 tttttcaaat aagcaaaaat tcctaaaaca caacaatgaa agcgataact aagaaaatcc
       61 ttacataacg atttttctta ttaaaattgt tttttgaact tcgcttattt tttgtgagca
      121 tatctgttta cttacttttt gtaaggtgct atacttcatt tcgtaatcaa ttaaaaaaat
      181 gcaaaacgca aatcaaagga gaaattttat tatgacaact tttactgaca ctttgaaaaa
      241 ccgccgttca atctatcatc taggacgtaa tgtttcactt tctaatgaag aattaacaac
      301 tttgatcaaa gaagcaatta aagaaagccc aactgctttc aatgctcaat caacacgcgc
      361 agttatcctt ttcggagatg cacatgaaaa actttgggaa atcactgaag aagcacttcg
      421 cccattgact ccagcagaag ctttcccaaa tacacaaaac aaattagctg gtttcaaaaa
      481 tggttatggt acagtattat tcttcaaaga tactgacgtg gtaaaaggtc tacaagaaca
      541 atttgaatta tatgcagaca acttcccaga ttggtcagaa caatcaaacg gaatcgcaac
      601 agctaatact tgggtagcat tagttgataa aggattgggc gcaaacttgc aacattacaa
      661 cccagttatt gacgaagcag ttgcaaaaga atggaatatc ccttcaaact ggaaactacg
      721 ttcgcaatta gtattcggtt caccagaaac accggctgag gaaaaagaat acatgaacga
      781 tgccgatcgc ttccgcgtat ttggataatt ttatttgaaa taaaaaacag aggttgtgac
      841 aaa
//
LOCUS       DACETN010000206          792 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DACETN010000206 DACETN010000000
VERSION     DACETN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.214"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..781
                     /locus_tag="IXF98_002660"
     CDS             <1..781
                     /locus_tag="IXF98_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1556919.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          244 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct tt
//
LOCUS       DACETN010000207          735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DACETN010000207 DACETN010000000
VERSION     DACETN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.215"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            27..>735
                     /locus_tag="IXF98_002661"
     CDS             27..>735
                     /locus_tag="IXF98_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556920.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          260 a          127 c          139 g          209 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taataataga gtcttatttc aaaatgaatc aatctgttgc taaaactgcc cattgcttga
      121 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
      181 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      241 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      421 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      541 aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga
      601 aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg
      661 taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      721 aacagatcag taatg
//
LOCUS       DACETN010000208          731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DACETN010000208 DACETN010000000
VERSION     DACETN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.216"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            1..731
                     /locus_tag="IXF98_002662"
                     /pseudo
     CDS             1..731
                     /locus_tag="IXF98_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          274 a          125 c          139 g          193 t
ORIGIN      
        1 gaactacgta aaagaaacca gcaattagaa atggaaaatg atattttaaa gcaagcagcg
       61 ctgatattcg gacgaagaga caagtaatcg atgcgaataa gcatctttac cctatatcag
      121 cgatgtgcag aatattaggt ctatcacgtc agtcctatta ttatcaatca aaaccaaaga
      181 aagacgaatc agaacttgaa gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct
      241 acggctcaag aaaaataaaa aaagccttat caaaacgagg cattcagatc agccgacgaa
      301 aaattagtag aatcatgaaa aatagaggat taaaatcgag ctatactgtt gcttatttta
      361 aagtacatca ttctacttgc aatgaagcca aaacgacaaa cgtattgaat cgtaaattct
      421 taagagacaa cccattagaa gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa
      481 aatggaatta tgtctgtttc attttggatc tgttcaatcg agaaattctc ggctattctt
      541 gtggagaaca taaagatgcc gttctagtaa aaaaagcatt tagccgtatc aaacaacctc
      601 tgacagaggt tgagattttt catactgatc gtggaaaaga gtttgataac caagctattg
      661 atgaattatt aacaactttt gacatcaatc gatcattgag tcataaaggc tgtccttttg
      721 ataatgccgt a
//
LOCUS       DACETN010000209          718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DACETN010000209 DACETN010000000
VERSION     DACETN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.217"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..696
                     /locus_tag="IXF98_002663"
     CDS             <1..696
                     /locus_tag="IXF98_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1556921.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          241 a          146 c          127 g          204 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatc
//
LOCUS       DACETN010000210          686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DACETN010000210 DACETN010000000
VERSION     DACETN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.218"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            116..331
                     /locus_tag="IXF98_002664"
     CDS             116..331
                     /locus_tag="IXF98_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1556922.1"
                     /translation="MKKKLVKGSVICSMIGIGFIVIGTNVEAATYYGNGVYCNKQKCW
                     VNWGQAWSKGVKRWGDNLFGSFSGGRI"
     gene            332..673
                     /locus_tag="IXF98_002665"
     CDS             332..673
                     /locus_tag="IXF98_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1556923.1"
                     /translation="MIDIRETKKIIHELYNSLMKRDKTKAILDITDVLLQVYKKIDSE
                     KYPEILINKLVNYIYIVGFDNKIHFLGNDEKLLIELGDISKKAGINSKYKANFTDKSQ
                     FYSYSEKVPIR"
BASE COUNT          260 a           58 c          132 g          236 t
ORIGIN      
        1 attttgagtg tagcacaaat gactttttta gttgtctagc ttaattttac aatcggcgct
       61 atataatttc tacatagaag acgttttcta atttttattt tcgaggtgaa tattaatgaa
      121 aaagaaatta gttaaaggct cagttatttg tagcatgatt gggattggtt ttatagtaat
      181 aggaacaaat gtagaagctg ccacgtatta cggaaatggt gtgtattgta ataagcaaaa
      241 atgttgggta aattggggac aagcttggtc aaaaggggtt aaacgttggg gagataattt
      301 gtttggtagt tttagtggag gaaggattta aatgattgat attagggaaa caaaaaaaat
      361 aattcatgaa ttgtataata gtttaatgaa gcgtgataag accaaagcta tattagatat
      421 tacagatgtt ttgttgcaag tctataaaaa aatagatagc gaaaaatatc cagaaatatt
      481 aataaacaaa ttggtgaatt atatctatat tgttggattt gacaataaaa ttcatttttt
      541 aggaaatgat gaaaagttat taatagaatt gggagatata tctaaaaagg ctggaattaa
      601 cagtaaatat aaagctaatt ttacagataa atctcaattc tatagttatt ctgaaaaagt
      661 acctattaga taattatatt tcccta
//
LOCUS       DACETN010000211          684 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DACETN010000211 DACETN010000000
VERSION     DACETN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.219"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>684
                     /locus_tag="IXF98_002666"
     CDS             <1..>684
                     /locus_tag="IXF98_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1556924.1"
                     /translation="RIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRFRKGITMSEIA
                     DLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAK
                     EAIHIAVGIRPDGSKKVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMA
                     GAVQRFYPKARFQHCCVHVSRNIVHKVCVKDRKEICDDFKAVYQASSKEEANTFLGSM
                     IEKWQKTYP"
BASE COUNT          199 a          150 c          158 g          177 t
ORIGIN      
        1 atcgcattgg ttttaacacg ggtaattctc gtaacggctc ctatgaccgt acggtcaaga
       61 ccgagtacgg ggaacttcat ctccagattc cgcgcgaccg cttccgaaaa ggtattacca
      121 tgtcggaaat cgcagacttg attgagaaaa tgtatgggca tcactacacg ccccaaacca
      181 tgtccaatat gactaaatca tttacggaag aagtcacttc atttaaggaa cgtgagcttc
      241 atgaccgtta tgccgctatt tatatggacg caacctatat ccctttaaag cggaaaaccg
      301 tcgccaagga agccattcat atcgcagttg gcattcgccc ggacggttca aagaaagtgt
      361 tgagctatgc gattgcacca actgaatcta tcacgatttg ggaagagatt ttaatagacc
      421 tccaggaacg tggcgtgaaa aacgtcctcc ttttcatcac ggatggctta aaggggatgg
      481 caggggccgt gcagcggttc taccccaaag cccgttttca acattgctgt gtgcacgttt
      541 cccgtaatat tgttcataaa gtatgtgtca aagatcgaaa agaaatctgt gatgatttta
      601 aggctgttta tcaagctagc tcaaaggaag aggcaaatac ctttttaggg agtatgattg
      661 agaagtggca gaaaacttat ccta
//
LOCUS       DACETN010000212          574 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DACETN010000212 DACETN010000000
VERSION     DACETN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.220"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..572
                     /locus_tag="IXF98_002667"
     CDS             <1..572
                     /locus_tag="IXF98_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1556925.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          248 a           71 c           94 g          161 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACETN010000213          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DACETN010000213 DACETN010000000
VERSION     DACETN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.221"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(5..90)
                     /locus_tag="IXF98_002668"
     tRNA            complement(5..90)
                     /locus_tag="IXF98_002668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXF98_002669"
     tRNA            complement(107..178)
                     /locus_tag="IXF98_002669"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXF98_002670"
     tRNA            complement(190..262)
                     /locus_tag="IXF98_002670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXF98_002671"
     tRNA            complement(280..361)
                     /locus_tag="IXF98_002671"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXF98_002672"
     tRNA            complement(369..441)
                     /locus_tag="IXF98_002672"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXF98_002673"
     tRNA            complement(445..517)
                     /locus_tag="IXF98_002673"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACETN010000214          520 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DACETN010000214 DACETN010000000
VERSION     DACETN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.222"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>520
                     /locus_tag="IXF98_002674"
     rRNA            <1..>520
                     /locus_tag="IXF98_002674"
                     /product="23S ribosomal RNA"
BASE COUNT          149 a          100 c          158 g          113 t
ORIGIN      
        1 agcccgttaa tgggtgatgg cgtgcctttt gtagaatgaa ccggcgagtt acgattgcat
       61 gcgaggttaa gttgaagaga cggagccgca gcgaaagcga gtctgaatag ggcgtttgag
      121 tatgtagtcg tagacccgaa accatgtgat ctacccatgt ccaggttgaa ggtgcggtaa
      181 aacgcactgg aggaccgaac ccacgtacgt tgaaaagtgc ggggatgagg tgtgggtagc
      241 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
      301 cctcggaatt gagaatgatg gaggtagagc actgtttgga ctaggggccc atctcgggtt
      361 accgaattca gataaactcc gaatgccatt cattcatatc cgggagtcag actgtgagtg
      421 ataagatcca tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaatatat
      481 gttaagtgga aaaggatgtg gggttgcaca gacaactagg
//
LOCUS       DACETN010000215          507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DACETN010000215 DACETN010000000
VERSION     DACETN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.223"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..200)
                     /locus_tag="IXF98_002675"
     CDS             complement(<1..200)
                     /locus_tag="IXF98_002675"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556926.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRP"
     gene            complement(311..>507)
                     /locus_tag="IXF98_002676"
     CDS             complement(311..>507)
                     /locus_tag="IXF98_002676"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556927.1"
                     /translation="SVRGLAKEYGLSEATIYKWKNLYLPDQSTGLTGKEVAELRKENA
                     RLNEELEILKKAAAIFSRKT"
BASE COUNT          151 a           91 c           73 g          192 t
ORIGIN      
        1 tgaggtctat attttttaac tacaatcgac cttaaattta attgtttcat tagcttttgg
       61 acatgtttaa gagatactga aatcccttct tttaataaaa cttgatggat ctttgtggca
      121 ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct tctttttcaa cttattattt
      181 ctgatttctg tagctgtcaa cggtttattc ttataaaaat aatagacact tctagggatt
      241 tctaataacc gacacaacaa agaaacagtg taatccttac accatttttc aataaattga
      301 acaagcgaat ttaggttttc cgagagaata tggctgcggc tttttttaag atttcaagtt
      361 cctcatttaa acgagcattt tcttttctca gttcagctac ttcttttcca gtcagtcctg
      421 tggactgatc aggtaaatat aaattcttcc atttgtaaat cgttgcttca gataagccat
      481 attctttcgc taaccctcga acagaac
//
LOCUS       DACETN010000216          496 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DACETN010000216 DACETN010000000
VERSION     DACETN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.224"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1..381)
                     /locus_tag="IXF98_002677"
                     /pseudo
     CDS             complement(1..381)
                     /locus_tag="IXF98_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT          158 a           83 c           73 g          182 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag ctggtgttag tatcaaatct tagacagctt ttcgtagaga
      421 caagaataaa ttaaacttat ctacgagagg atgaattttt atgacccgat atgaagaaaa
      481 ttttaaacaa atgatt
//
LOCUS       DACETN010000217          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DACETN010000217 DACETN010000000
VERSION     DACETN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.225"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>487)
                     /locus_tag="IXF98_002678"
     CDS             complement(<1..>487)
                     /locus_tag="IXF98_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1556928.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACETN010000218          472 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DACETN010000218 DACETN010000000
VERSION     DACETN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.226"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            30..102
                     /locus_tag="IXF98_002679"
     tRNA            30..102
                     /locus_tag="IXF98_002679"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Asp,seq:gtc)
     gene            106..178
                     /locus_tag="IXF98_002680"
     tRNA            106..178
                     /locus_tag="IXF98_002680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:139..141,aa:Phe,seq:gaa)
     gene            183..253
                     /locus_tag="IXF98_002681"
     tRNA            183..253
                     /locus_tag="IXF98_002681"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:215..217,aa:Gly,seq:tcc)
     gene            266..339
                     /locus_tag="IXF98_002682"
     tRNA            266..339
                     /locus_tag="IXF98_002682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Ile,seq:gat)
     gene            351..422
                     /locus_tag="IXF98_002683"
     tRNA            351..422
                     /locus_tag="IXF98_002683"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Glu,seq:ttc)
BASE COUNT           97 a          107 c          125 g          143 t
ORIGIN      
        1 tgctcccgca attaaaaact aaaaacattg gttccgtggt gtaggggtta acatgcctgc
       61 ctgtcacgca ggagatcgcg ggttcgattc ccgtcggaac cgtttggctc ggtagctcag
      121 ttggtagagc aatggattga agctccatgt gtcggcagtt cgattctgtc ccgcgccatt
      181 atgcgggtgt agtttagtgg taaaaccaca gccttccaag ctgttgtcgc gagttcgatt
      241 ctcgtcaccc gcttttagtt tttatgggcc tatagctcag ctggttagag cgcacgcctg
      301 ataagcgtga ggtcgatggt tcgagtccat ttaggcccat ttattttatt ggcccgttgg
      361 tcaagcggtt aagacaccgc cctttcacgg cggtaacacg ggttcgaatc ccgtacgggt
      421 catggaagat actttttgag tatctttttc tttttatctg aaagaatgat tt
//
LOCUS       DACETN010000219          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DACETN010000219 DACETN010000000
VERSION     DACETN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.227"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(20..91)
                     /locus_tag="IXF98_002684"
     tRNA            complement(20..91)
                     /locus_tag="IXF98_002684"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXF98_002685"
     tRNA            complement(113..200)
                     /locus_tag="IXF98_002685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXF98_002686"
     tRNA            complement(225..298)
                     /locus_tag="IXF98_002686"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXF98_002687"
     tRNA            complement(312..382)
                     /locus_tag="IXF98_002687"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXF98_002688"
     tRNA            complement(384..459)
                     /locus_tag="IXF98_002688"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACETN010000220          440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DACETN010000220 DACETN010000000
VERSION     DACETN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.228"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            11..>440
                     /locus_tag="IXF98_002689"
     CDS             11..>440
                     /locus_tag="IXF98_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1556929.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACETN010000221          438 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DACETN010000221 DACETN010000000
VERSION     DACETN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.229"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            42..>438
                     /locus_tag="IXF98_002690"
     CDS             42..>438
                     /locus_tag="IXF98_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1556930.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          137 a           81 c           86 g          134 t
ORIGIN      
        1 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
       61 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      121 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      181 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      241 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      301 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      361 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      421 cttccatgac ggaaatcg
//
LOCUS       DACETN010000222          427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DACETN010000222 DACETN010000000
VERSION     DACETN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.230"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            55..315
                     /locus_tag="IXF98_002691"
     CDS             55..315
                     /locus_tag="IXF98_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556931.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          157 a           45 c           59 g          166 t
ORIGIN      
        1 actctcaatt attttaatat aagtacttca gatttaataa aggagaaata tctaatgaaa
       61 aaaattgatt taataaatat gataggtatg ttaataggga tattagtaaa tatagtaatt
      121 ttcacagatt ggctcggggt gttattttct aatcttattc ccatacttat tataggcatt
      181 tgtggaatta tactttcaat attagaactt tttgaaagca gaaatacaat gaatagaata
      241 tttgcttgta ttatattaat tgttaatcta ttgcctatgg tctattttac ttttttatat
      301 tttgcactgg gatgaaataa attaaccaaa agtttctttt tcttataatg tgtatcaata
      361 ttaaaactga aaaagatatg gataatagga tcaaagccct cattggaaca ctcaatatat
      421 tatcaga
//
LOCUS       DACETN010000223          419 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DACETN010000223 DACETN010000000
VERSION     DACETN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.231"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>419
                     /locus_tag="IXF98_002692"
     CDS             <1..>419
                     /locus_tag="IXF98_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556932.1"
                     /translation="AFPIRCSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQA
                     FRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAI
                     DIENKLNHWFESVPKNLFKSITFDCGKEFSNWKQISN"
BASE COUNT          142 a           71 c           80 g          126 t
ORIGIN      
        1 agcgtttcct attcgttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt
       61 taatccttct gacttaccga tgaaaggcaa gcgtaaacca aatggacatc aagaaagacg
      121 tggaaaacaa gctttccgtc gctctattca tgaacgtgaa aaagattata gccaattctc
      181 aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ttgaaacata aaagtgctgt
      241 aattacctta gttgagcgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca
      301 agcgattgat attgaaaaca aattaaatca ttggtttgaa tctgtaccga aaaatctatt
      361 caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatg
//
LOCUS       DACETN010000224          404 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DACETN010000224 DACETN010000000
VERSION     DACETN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.232"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>404
                     /locus_tag="IXF98_002693"
     CDS             <1..>404
                     /locus_tag="IXF98_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556933.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACETN010000225          399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DACETN010000225 DACETN010000000
VERSION     DACETN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.233"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..382
                     /locus_tag="IXF98_002694"
     CDS             <1..382
                     /locus_tag="IXF98_002694"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1556934.1"
                     /translation="KRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWL
                     KTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNR
                     NRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          159 a           75 c           69 g           96 t
ORIGIN      
        1 aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca
       61 cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt
      121 ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta
      181 tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac
      241 tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta
      301 taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt
      361 agcaaaagaa aaaatggctt aaccgtctct actaaaatg
//
LOCUS       DACETN010000226          381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DACETN010000226 DACETN010000000
VERSION     DACETN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.234"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..94)
                     /locus_tag="IXF98_002695"
     tRNA            complement(21..94)
                     /locus_tag="IXF98_002695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IXF98_002696"
     tRNA            complement(108..181)
                     /locus_tag="IXF98_002696"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IXF98_002697"
     tRNA            complement(209..281)
                     /locus_tag="IXF98_002697"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IXF98_002698"
     tRNA            complement(301..374)
                     /locus_tag="IXF98_002698"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACETN010000227          376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DACETN010000227 DACETN010000000
VERSION     DACETN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.235"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            82..>376
                     /locus_tag="IXF98_002699"
     CDS             82..>376
                     /locus_tag="IXF98_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1556935.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT          133 a           62 c           76 g          105 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccatt
//
LOCUS       DACETN010000228          359 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DACETN010000228 DACETN010000000
VERSION     DACETN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.236"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>359
                     /locus_tag="IXF98_002700"
     CDS             <1..>359
                     /locus_tag="IXF98_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556936.1"
                     /translation="KRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVG
                     IHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEF
                     SNWKAISNQHDIDIYFA"
BASE COUNT          120 a           69 c           62 g          108 t
ORIGIN      
        1 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
       61 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
      121 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
      181 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
      241 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
      301 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcg
//
LOCUS       DACETN010000229          334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DACETN010000229 DACETN010000000
VERSION     DACETN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.237"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          100 a           54 c           73 g          107 t
ORIGIN      
        1 gaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt
       61 tcctcatatt ttgccgtttg ttgagctaat accgaaggtc agctgattga acaccgttta
      121 agaaagaggt cgtgagcctg tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca
      181 gcttctcatc tttgaggaat atcgtgactt gtggacgagg cgttgataag tgaatcatct
      241 ttttgttacc tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgccctta
      301 atgaggacag tcggaacagg tttgtcattt ttat
//
LOCUS       DACETN010000230          311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DACETN010000230 DACETN010000000
VERSION     DACETN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.238"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>311
                     /locus_tag="IXF98_002701"
     CDS             <1..>311
                     /locus_tag="IXF98_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ1556937.1"
                     /translation="YAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVC
                     T"
BASE COUNT          105 a           62 c           62 g           82 t
ORIGIN      
        1 tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt agtcacagat
       61 aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt ttatcaaggg
      121 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
      181 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      241 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      301 gtctgtactg a
//
LOCUS       DACETN010000231          301 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DACETN010000231 DACETN010000000
VERSION     DACETN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.239"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>301
                     /locus_tag="IXF98_002702"
     rRNA            <1..>301
                     /locus_tag="IXF98_002702"
                     /product="23S ribosomal RNA"
BASE COUNT           93 a           59 c           87 g           62 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 t
//
LOCUS       DACETN010000232          299 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DACETN010000232 DACETN010000000
VERSION     DACETN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.240"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>299
                     /locus_tag="IXF98_002703"
     CDS             <1..>299
                     /locus_tag="IXF98_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556938.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DACETN010000233          296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DACETN010000233 DACETN010000000
VERSION     DACETN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.241"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>296)
                     /locus_tag="IXF98_002704"
     CDS             complement(<1..>296)
                     /locus_tag="IXF98_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556939.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT           80 a           60 c           50 g          106 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc aaacaccgtc
      241 gtttctctaa atcggcgaac ataattcttt ccaaaagttg agaaatgggg gatttt
//
LOCUS       DACETN010000234          291 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DACETN010000234 DACETN010000000
VERSION     DACETN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.242"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            76..>291
                     /locus_tag="IXF98_002705"
     CDS             76..>291
                     /locus_tag="IXF98_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1556940.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          111 a           55 c           50 g           75 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt t
//
LOCUS       DACETN010000235          276 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DACETN010000235 DACETN010000000
VERSION     DACETN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.243"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          120 a           49 c           30 g           77 t
ORIGIN      
        1 aagggacttc cttttttcta tttacacaaa atattctatt ttgttatcac tgttgaatca
       61 tcatttataa gtgaacgtaa aagataatag atacccaaaa acagaaagaa aaataacggt
      121 aatacacgga gtaaaacaaa cggagtttca aaaatgaacg ctttccaaaa ccacataccc
      181 tccatttccc ataaaccttg ggtaaaatac catgatttaa agttcaatat cttatccaaa
      241 ttccaagaat tactactaaa aaaaaataaa gaaata
//
LOCUS       DACETN010000236          273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DACETN010000236 DACETN010000000
VERSION     DACETN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.244"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>273
                     /locus_tag="IXF98_002706"
     CDS             <1..>273
                     /locus_tag="IXF98_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556941.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINH"
BASE COUNT          121 a           37 c           43 g           72 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cat
//
LOCUS       DACETN010000237          272 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DACETN010000237 DACETN010000000
VERSION     DACETN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.245"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(6..272)
                     /locus_tag="IXF98_002707"
                     /pseudo
     CDS             complement(6..272)
                     /locus_tag="IXF98_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           82 a           58 c           43 g           89 t
ORIGIN      
        1 tgctacttct caatcatact ccctaaaaag gtatttgcct cttcctttga gctagcttga
       61 taaacagcct taaaatcatc atagatttct tttcgatctt tgacacgtac tttatgagca
      121 atattacggg aaacgtgaac acagcaatgt tggtatttag ctttaggata aatctggtga
      181 atcgtatctt tcatgccttt taagccatcg gtaataaaca gtaagacatc atgaacccct
      241 ctggagtaaa tgtcctgtaa caactcattc ca
//
LOCUS       DACETN010000238          263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DACETN010000238 DACETN010000000
VERSION     DACETN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.246"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           97 a           41 c           37 g           88 t
ORIGIN      
        1 ttttaatggc ttcaaatata aaagagttta aagcgtaaca cacttatggg gaagtggttt
       61 ggggtgcgct ttaaactctt ctttattatt atctcacaat ttaacccaaa tgtctttcta
      121 tttaaaaatc aaagtaaaac ttttcaaata tacagaagta taactatgtg aaacatcctt
      181 tcattaatcc ataaaaggat acataaaaaa gccactcatt tgagtggcaa tgaagaaaag
      241 ctttagcttg tataatactc ttc
//
LOCUS       DACETN010000239          249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DACETN010000239 DACETN010000000
VERSION     DACETN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.247"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>249
                     /locus_tag="IXF98_002708"
     CDS             <1..>249
                     /locus_tag="IXF98_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556942.1"
                     /translation="SKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIVEA
                     TYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           71 a           50 c           47 g           81 t
ORIGIN      
        1 gtctaaaaac aatacgaatg gtagaaatca atttgcgatg cttacgattg atgatctagt
       61 tcctcaagat catctcgttc gaaagattga tgccgcgctt gattttgaat ttatttatcc
      121 aatcgtcgaa gccacttact ccgatttagg tcgcccaagt atcgatccag tgattctgat
      181 caaattggtg tttattcagt atttattcgg tatccgttcc atgcgacaaa ccattaagga
      241 agtcgatac
//
LOCUS       DACETN010000240          244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DACETN010000240 DACETN010000000
VERSION     DACETN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.248"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>244)
                     /locus_tag="IXF98_002709"
     rRNA            complement(<1..>244)
                     /locus_tag="IXF98_002709"
                     /product="23S ribosomal RNA"
BASE COUNT           52 a           81 c           49 g           62 t
ORIGIN      
        1 ttggacagac atttccgatc gtctgcattc cttagcctcc tgcgtccctc cattgctcaa
       61 acaaaaacaa cgggtacagg aatatcaacc tgttatccat cgcctacgcc tgtcggcctc
      121 ggcttaggtc ccgactaacc ctgggcggac gagccttccc caggaaacct tagtcattcg
      181 gtggacagga ttctcacctg tctttcgcta ctcataccgg cattctcact tctaagcgct
      241 ccag
//
LOCUS       DACETN010000241          236 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DACETN010000241 DACETN010000000
VERSION     DACETN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.249"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            26..>236
                     /locus_tag="IXF98_002710"
     CDS             26..>236
                     /locus_tag="IXF98_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1556943.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRL"
BASE COUNT           86 a           48 c           39 g           63 t
ORIGIN      
        1 aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag aaacattaga
       61 tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa aatacaaagg
      121 gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat gcatccactg
      181 taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc ggttgt
//
LOCUS       DACETN010000242          232 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DACETN010000242 DACETN010000000
VERSION     DACETN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.250"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           79 a           35 c           45 g           73 t
ORIGIN      
        1 gagaaaaagc atggaatgcg ctgcatcagg tatcgaggct tagaaaaaaa tcaggtgcat
       61 acgatgcttg tttttgccga aatgagtctg aaaaaattgg tcacttagca gtgggagcat
      121 atgtcttcat attttttatt atacaaaaat agacaaaagt atcagtctac actcttagta
      181 aagaggaaac tgatactttt gtctacagta tgatatttct tacatctact tt
//
LOCUS       DACETN010000243          227 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DACETN010000243 DACETN010000000
VERSION     DACETN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.251"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            1..227
                     /locus_tag="IXF98_002711"
                     /pseudo
     CDS             1..227
                     /locus_tag="IXF98_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DACETN010000244          224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DACETN010000244 DACETN010000000
VERSION     DACETN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.252"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>224
                     /locus_tag="IXF98_002712"
     CDS             <1..>224
                     /locus_tag="IXF98_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1556944.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DACETN010000245          218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DACETN010000245 DACETN010000000
VERSION     DACETN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.253"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>218
                     /locus_tag="IXF98_002713"
     CDS             <1..>218
                     /locus_tag="IXF98_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556945.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT           66 a           39 c           44 g           69 t
ORIGIN      
        1 tcttttattg cgaaaaattg tcaggtatct ttgaccaccg ttattcgtac gttgaaagag
       61 tttaaaagct acttaccaaa gcaatctaag aggattctcc ccagagtatt gatggttgat
      121 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggcgaa
      181 acaggacaat taattgatgt cttgcctacg cgtaaatt
//
LOCUS       DACETN010000246          217 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DACETN010000246 DACETN010000000
VERSION     DACETN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.254"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>217
                     /locus_tag="IXF98_002714"
     CDS             <1..>217
                     /locus_tag="IXF98_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556946.1"
                     /translation="LNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHD
                     VKVLETLSEEANLPNILGDKGYISHKI"
BASE COUNT           77 a           40 c           36 g           64 t
ORIGIN      
        1 tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat ttttatggtc
       61 ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca atcacgaatc
      121 caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat cttcccaata
      181 tcctagggga caaaggatat attagccata aaatcca
//
LOCUS       DACETN010000247          214 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DACETN010000247 DACETN010000000
VERSION     DACETN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.255"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           87 a           25 c           38 g           64 t
ORIGIN      
        1 aaagcttcga aatccattgg catttcgttg gaataccttg atatgattat tcaaatataa
       61 aatatagaga gaagagagag agggaagata tttataaagg gcaaagttat gtttactcaa
      121 cttaggaaat agaaatagat caatttatat tctcttgtca atcgaatatg aaccttagca
      181 ctatatacca aatatagtaa aatgctgaaa gatg
//
LOCUS       DACETN010000248          209 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DACETN010000248 DACETN010000000
VERSION     DACETN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.256"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>209
                     /locus_tag="IXF98_002715"
     CDS             <1..>209
                     /locus_tag="IXF98_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1556947.1"
                     /translation="RWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRT
                     PLPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT           51 a           43 c           44 g           71 t
ORIGIN      
        1 cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag
       61 aaaaatctat cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat
      121 ccttttcgga caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt
      181 gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DACETN010000249          208 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DACETN010000249 DACETN010000000
VERSION     DACETN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.denovo.257"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            50..>208
                     /locus_tag="IXF98_002716"
     CDS             50..>208
                     /locus_tag="IXF98_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1556948.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQE"
BASE COUNT           72 a           29 c           46 g           61 t
ORIGIN      
        1 actatcaaat aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt
       61 taaaggaaag caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta
      121 taaccttagc tatcgtgaag ttcaagaaat cttatatgat cgtggcatta acgtttctca
      181 tacgacgatt tatcgttggg tgcaagaa
//
LOCUS       DACETN010000250        49927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.006, whole genome
            shotgun sequence.
ACCESSION   DACETN010000250 DACETN010000000
VERSION     DACETN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.006"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            173..1483
                     /locus_tag="IXF98_002717"
     CDS             173..1483
                     /locus_tag="IXF98_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1556949.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            1726..2001
                     /locus_tag="IXF98_002718"
     CDS             1726..2001
                     /locus_tag="IXF98_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1556950.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            2236..3282
                     /locus_tag="IXF98_002719"
     CDS             2236..3282
                     /locus_tag="IXF98_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556951.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            3284..4543
                     /locus_tag="IXF98_002720"
     CDS             3284..4543
                     /locus_tag="IXF98_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1556952.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            4549..5130
                     /locus_tag="IXF98_002721"
     CDS             4549..5130
                     /locus_tag="IXF98_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1556953.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(5212..6087)
                     /locus_tag="IXF98_002722"
     CDS             complement(5212..6087)
                     /locus_tag="IXF98_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1556954.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            6235..6567
                     /locus_tag="IXF98_002723"
     CDS             6235..6567
                     /locus_tag="IXF98_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1556955.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            6679..7887
                     /locus_tag="IXF98_002724"
     CDS             6679..7887
                     /locus_tag="IXF98_002724"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1556956.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            8041..8496
                     /locus_tag="IXF98_002725"
     CDS             8041..8496
                     /locus_tag="IXF98_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556957.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            8858..9133
                     /locus_tag="IXF98_002726"
     CDS             8858..9133
                     /locus_tag="IXF98_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556958.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            9146..11224
                     /locus_tag="IXF98_002727"
     CDS             9146..11224
                     /locus_tag="IXF98_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1556959.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            11384..13270
                     /locus_tag="IXF98_002728"
     CDS             11384..13270
                     /locus_tag="IXF98_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1556960.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            13282..14229
                     /locus_tag="IXF98_002729"
     CDS             13282..14229
                     /locus_tag="IXF98_002729"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1556961.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            14249..14776
                     /locus_tag="IXF98_002730"
     CDS             14249..14776
                     /locus_tag="IXF98_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1556962.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14839..15492)
                     /locus_tag="IXF98_002731"
     CDS             complement(14839..15492)
                     /locus_tag="IXF98_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1556963.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            15624..16466
                     /locus_tag="IXF98_002732"
     CDS             15624..16466
                     /locus_tag="IXF98_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1556964.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16624..17520
                     /locus_tag="IXF98_002733"
     CDS             16624..17520
                     /locus_tag="IXF98_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1556965.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            17523..18236
                     /locus_tag="IXF98_002734"
     CDS             17523..18236
                     /locus_tag="IXF98_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1556966.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            18256..18774
                     /gene="msrA"
                     /locus_tag="IXF98_002735"
     CDS             18256..18774
                     /gene="msrA"
                     /locus_tag="IXF98_002735"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1556967.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            18771..18998
                     /locus_tag="IXF98_002736"
     CDS             18771..18998
                     /locus_tag="IXF98_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1556968.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            19150..19701
                     /gene="lepB"
                     /locus_tag="IXF98_002737"
     CDS             19150..19701
                     /gene="lepB"
                     /locus_tag="IXF98_002737"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1556969.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(19784..20326)
                     /locus_tag="IXF98_002738"
     CDS             complement(19784..20326)
                     /locus_tag="IXF98_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1556970.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(20563..20961)
                     /locus_tag="IXF98_002739"
     CDS             complement(20563..20961)
                     /locus_tag="IXF98_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1556971.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            21221..22081
                     /gene="ylqF"
                     /locus_tag="IXF98_002740"
     CDS             21221..22081
                     /gene="ylqF"
                     /locus_tag="IXF98_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1556972.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            22074..22841
                     /locus_tag="IXF98_002741"
     CDS             22074..22841
                     /locus_tag="IXF98_002741"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1556973.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            22897..23754
                     /gene="dprA"
                     /locus_tag="IXF98_002742"
     CDS             22897..23754
                     /gene="dprA"
                     /locus_tag="IXF98_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1556974.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            23876..25954
                     /gene="topA"
                     /locus_tag="IXF98_002743"
     CDS             23876..25954
                     /gene="topA"
                     /locus_tag="IXF98_002743"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1556975.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            25923..27299
                     /gene="trmFO"
                     /locus_tag="IXF98_002744"
     CDS             25923..27299
                     /gene="trmFO"
                     /locus_tag="IXF98_002744"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1556976.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            27497..28402
                     /gene="xerC"
                     /locus_tag="IXF98_002745"
     CDS             27497..28402
                     /gene="xerC"
                     /locus_tag="IXF98_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1556977.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            28439..28987
                     /gene="hslV"
                     /locus_tag="IXF98_002746"
     CDS             28439..28987
                     /gene="hslV"
                     /locus_tag="IXF98_002746"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1556978.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            29001..30401
                     /gene="hslU"
                     /locus_tag="IXF98_002747"
     CDS             29001..30401
                     /gene="hslU"
                     /locus_tag="IXF98_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1556979.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            30419..31210
                     /gene="codY"
                     /locus_tag="IXF98_002748"
     CDS             30419..31210
                     /gene="codY"
                     /locus_tag="IXF98_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1556980.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            31292..32167
                     /locus_tag="IXF98_002749"
     CDS             31292..32167
                     /locus_tag="IXF98_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1556981.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(32299..32937)
                     /gene="plsY"
                     /locus_tag="IXF98_002750"
     CDS             complement(32299..32937)
                     /gene="plsY"
                     /locus_tag="IXF98_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1556982.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            33064..33498
                     /locus_tag="IXF98_002751"
     CDS             33064..33498
                     /locus_tag="IXF98_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1556983.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            33664..35709
                     /gene="parE"
                     /locus_tag="IXF98_002752"
     CDS             33664..35709
                     /gene="parE"
                     /locus_tag="IXF98_002752"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1556984.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            35721..38171
                     /gene="parC"
                     /locus_tag="IXF98_002753"
     CDS             35721..38171
                     /gene="parC"
                     /locus_tag="IXF98_002753"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1556985.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            38448..40679
                     /gene="pflB"
                     /locus_tag="IXF98_002754"
     CDS             38448..40679
                     /gene="pflB"
                     /locus_tag="IXF98_002754"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1556986.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            40923..41684
                     /gene="pflA"
                     /locus_tag="IXF98_002755"
     CDS             40923..41684
                     /gene="pflA"
                     /locus_tag="IXF98_002755"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1556987.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            41769..42695
                     /locus_tag="IXF98_002756"
     CDS             41769..42695
                     /locus_tag="IXF98_002756"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1556988.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            42877..43770
                     /locus_tag="IXF98_002757"
     CDS             42877..43770
                     /locus_tag="IXF98_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1556989.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            43904..44029
                     /locus_tag="IXF98_002758"
     CDS             43904..44029
                     /locus_tag="IXF98_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1556990.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(44163..44795)
                     /locus_tag="IXF98_002759"
     CDS             complement(44163..44795)
                     /locus_tag="IXF98_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556991.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(44943..45485)
                     /locus_tag="IXF98_002760"
     CDS             complement(44943..45485)
                     /locus_tag="IXF98_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1556992.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            45620..46009
                     /locus_tag="IXF98_002761"
     CDS             45620..46009
                     /locus_tag="IXF98_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556993.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            46013..46777
                     /locus_tag="IXF98_002762"
     CDS             46013..46777
                     /locus_tag="IXF98_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1556994.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            46906..47853
                     /locus_tag="IXF98_002763"
     CDS             46906..47853
                     /locus_tag="IXF98_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1556995.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            47983..48723
                     /locus_tag="IXF98_002764"
     CDS             47983..48723
                     /locus_tag="IXF98_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1556996.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(49014..49682)
                     /locus_tag="IXF98_002765"
     CDS             complement(49014..49682)
                     /locus_tag="IXF98_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1556997.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT        17385 a         8525 c        10405 g        13612 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcct ttttcttttg aaaagcgatt
       61 agtatttcaa taaggaataa aaatacttcg tttaattgac aatctatgct tgaaagttgg
      121 caatgcattc gttaaactaa gtgagattgg aaacttgtga ggaggaaata atatggcaaa
      181 tccaacaatt gcaatcgttg gccgcccgaa cgtcggtaag tctacgattt ttaaccgcat
      241 cgctggtgag cgtatctcga ttgtcgaaga tactcctgga gtaactcgtg atcgtattta
      301 tgcaaaaggt gaatggttag gtcgagaatt cagcgtgatc gacacaggcg gaattgatct
      361 tggcgatgaa ccttttatgg atcagatcaa gcatcaagca gaaatcgcga ttgaagaagc
      421 agatgtaatc atttgtgtag tcagcggacg cgaaggcgtg acagacgcag atgagatggt
      481 tgcgaaaatt ttatacagaa gtaataaacc agtgatttta gcagtcaata aagtagataa
      541 tccagaaatg agaaacgata tctatgaatt ttattcctta gggctaggcg atccttatcc
      601 tgtttcagga agccatggac tagggattgg tgatatttta gatgaagcag tgaagtattt
      661 ctctgaagag actgaagaag aggacgacga cacgattaag tttagtttga ttggtcgccc
      721 aaatgttggt aaatcctctt tgatcaatgc gattttagga gaagaacgtg taattgtttc
      781 ggatattgaa ggaacgaccc gagacgcaat cgacacccac ttcgtttctg aaagtggtca
      841 gaaattctta atgatagata cagctggtat gcgtaaacgc ggaaaagtat acgaaaatac
      901 tgaaaaatac agcgtgatgc gtgcaatgcg tgccattgac cgttctgata tcgttttgat
      961 ggtactgaat gctgaagaag ggattcggga acaagacaag cgagtagcgg gttacgccca
     1021 tgaagctggt cgtggaatta ttatcgtcgt gaataaatgg gatcttgtaa agaaagaaac
     1081 caatacaatg cgtgatttcg aacaagagat tcgtgatgaa ttccggtatc ttgattacgc
     1141 tccaatcgtt tttgtttctg cagtgactaa acaacggttg gaacgtctgc ctgaaatgat
     1201 cgaacaagta agtatgaacc agaatcttcg tatctcttcg gctgtgttga atgatatcat
     1261 tatggatgct gtagcaatca atccaacacc aacagataaa ggaaaacgcc tgaagatttt
     1321 ttatgctaca caagtcgctg tcaaaccacc tacatttgtc gtttttgtca atgaagagga
     1381 attgatgcat ttttcttatg aacgtttctt agaaaatcaa atcagaaaag catttacttt
     1441 tgaagggacg tccatccgaa ttatccctcg taggagaaaa taggcatttt accatatcga
     1501 cgtttttttt tcaaaagaaa aagacagttt cgtttgttac gtttctatga ctaaaaaaag
     1561 catgtgaaaa ttggtaaaaa ccttgctatt aagcggttca tatgatatcg tgataacaga
     1621 atattgaagt taatcaatat ttctttttaa acttctggac cactcagaaa gttttaacta
     1681 aatgatattt ctatcatatc tcttgtggag gaggtgaatc tatccatggc aaacaaagca
     1741 gaattaatcg aaaaagttgc ttcagctact gatttaacta aaaaagatgc aactgcagca
     1801 gtagatgctg tattttcaac tattcaagat gctttagcta acggcgaaaa agttcaatta
     1861 atcggttttg gtaactttga agttcgcgaa cgcgcagctc gcaaaggtcg taacccacaa
     1921 actggtgaag aaatcgaaat tccagctagc aaagtacctg cattcaaacc aggtaaagca
     1981 ttaaaagacg ctgttaaata agacagttga aaaagcgctt gtggctctag ggctacaagt
     2041 gcttttcttt ttatttccta gcagaaaagg aataaataag agacagagat tccgaatccg
     2101 tatgtcatac atagaaaaca catgtatttt atttaatttt tatttatttg caagttatga
     2161 ggtgagcttt gctcttatct tttctagaat ttgcttacta atggtaaaat aaaaataaga
     2221 agaggaggga tgcgtatgga acttgattat caagtagaag atgcgttgac gaaagtgttt
     2281 tacggagaaa cgctagtcag tcagctaaga gaactttcca ttgatcagcc aatcttattt
     2341 ttaacgaatc agcgctatta tgatttgttt gcagataaga tcaacccgtt atttgcaaat
     2401 caagcaaaca atgactggta tatttgtgcc aacactcaat gcaatcattt aacagaatta
     2461 aagaacctgc ttgatttcgt caaacgctat cccgaaaatc aatccatgtt tatcatcgga
     2521 tttggaaatg aagggatcat ggatctggca ggtttttttc aaaaacatac aacccttgat
     2581 tcatcactat ggatgatccc tgtatcgatc cgttctctaa gccgagcatt aacaccgcag
     2641 cggacgattt tgcagctacc aaatcgtgta gtgctgcaaa ccatcaatct gccagaacgc
     2701 atcgtttatg atcaaacaat cagcgagcgg caggtggacg gaaagcagat tgactttttg
     2761 acttttatct gttgcggaat gatttgtgat catcgttttt tgcaaaatct gtataaaaac
     2821 tatccaaata aaaagcaatt aacgactcgg ccgtttgccg ggatgatcga agaaatgatc
     2881 tatttttatc aattagaggc agaaaacctg tcaaattatg gtaaactgtt tgaacaagct
     2941 ttttatctga ccgaaaatgg ccatctactt tctgctagta tgaaaaaatt tttaggaatg
     3001 atgtttcatt tcgtgtggaa tctccaagca tatccttttg atttccaact aaaaaacttt
     3061 tttgtgtggc tggacttgct tggctatcca atccactggc tggaacagat ttcaaagatg
     3121 gagtatttgg aaaaagttct ttttttggca gaaaaaagca aaaaaatact tgttttagaa
     3181 aaaataggta taatagacgg ataccaacat ccaaacgaaa aagaattatt gagaacaatg
     3241 acggtatttc aacaaatcgt cagtgagata agagggaatt gaaatgaata caaatagcga
     3301 aaaaatgttg caagctttat cagatgaaga cttgtcacaa gcacaaatct ttttagaaaa
     3361 agcattaaaa gaagacccag cagatatatt agctgaacta ggcgaagaat tgctggctat
     3421 tggctttttg atcgaagcaa aacagatttt tgaacaactg atcaaccaat acccagaaaa
     3481 tgacggactg aacatcccgt tagcagaaat tgcaatcgaa gataatgaga tcgacttagc
     3541 ttttgaatac ctggaaaaag tggataagca aagtgattac tatccgcaaa gcttattggc
     3601 gatagctgat ttgtaccaag ttatcggaat ccctgaagtg agtgaagcaa aattgaaaga
     3661 agcagctggg ttgcttcctg atgaaccatt gatccagttt gcgctagctg aattgtattt
     3721 ttcagttgat cgtttcaaag aggcagcagt ggtttatgaa acattgttgg caagcgggat
     3781 aaatgaaatt tcaggaatct cgatgcagga acgtttagga caatccttaa gtatgcaagg
     3841 agaattcgaa gcagctatcg tacctttaga agaagcactg aaagaagaaa ggacagatga
     3901 tcgactgttc cagctagctt ttacgaatct tcaattgaaa gaaaatcagc aggcaatcaa
     3961 ctatctggaa gaacttcgag aagttaatcc gcattatcaa tcgttatatc tatatctagg
     4021 acaagctctt caagaagaag aaatgattga agaagcacaa acagtgttag aagaaggaat
     4081 caaagaaaat ccttaccaag tcgaacttta tcacctggcc tcggaaaatg ctttccgtct
     4141 gcatgataga cagcgagcag aagaactttt actgaaagct ttagaagtag gagacaaaca
     4201 agatgaaaca ttacttactt taagtaatct ttacttggat gatgaaagat atgaagatgt
     4261 gatacaagtg atcaatcaga tggaagaaac tgctaatcct tatgcagaat ggaacctagc
     4321 ccatgcttat aatgaattag aagactttac cgttgcagct gtacactatg aacaggctta
     4381 tcatgaacta tcacatgaac cagatttctt gaaagagtat gcctttttct taagagaaga
     4441 agggcagctg aaaagagcgc aagaattatt gacttattat ttaacacttg aaccaggaga
     4501 tatggaggca ctgtcattat tagatgacct aacagagagg tgagaaaaat gctaattgat
     4561 gtacgggaaa agaagaattt tttaacgtgg atggtcaatc atgtatcttt tagccgaaga
     4621 gaagtttttt ggatcttgaa ttatcttgcc aatcatgaag cgattttaag caatgtccac
     4681 tttgttgaag gggcgaattt tgcggatcga ggactgcaga tcagtgatct atctgttgaa
     4741 ggggaaccca tgaaattgtt tttgcaggga aaagaattta cggacacaga ccagattttt
     4801 catgaaatcc gtttgaattg gaaaaagcct ttatatgtgg aatgtatatt cgaaaatgct
     4861 tggcagacaa gagaatatct atctatctta gaagacaatc cattttcttc ttgggattct
     4921 tcagtcagtg aagaagatac tgaattaatt gaacgatatt ttcaagaaaa agaagaagag
     4981 gcaaaactaa aaatgcttta tgctcaaatc gatcaagcac tagaagatgg agatagggat
     5041 gcttttttga aattatctga tgaagttaac cgaaagatcc ttttgcagac acaaaagaaa
     5101 agtaaccggg taagtgaaca taacaattag ttaaaaatag tatagtacta aaaaagacaa
     5161 aggtcagctg gatgtggaat ctttcctcat agcctatctt tgtctttttt cttatagttt
     5221 tttcagtaat cttgtctgtg gcttcaaata agtaaagcct tgcccttcca cttcatgcac
     5281 attgatgatt gaaaggaaag ctttttcgtc aatctcgtgg acgatcctct tagcttcagt
     5341 taattcacta gagctaacga ccatatagat gatttttttt gaattaccag agaatgcgcc
     5401 ttctccagaa aggaaagtca cacctctttg cagaccggtc atcagtagtg gtgcaatctc
     5461 ttcgcttttg ttggagacga caagtagacc ttttgcagaa tatccgccat ctaagatcga
     5521 atcaatcaca cgactgaaaa catacgaaac aatcaacgta tacatcatac gtttcaagtc
     5581 gatataggtt aatgaagcaa gtaagacgat gatatcaaat gccaataggg aacgccccat
     5641 agtgatgccg taattttgtt ctaaaatccg agcgataata tcactgccgc ctgttgttcc
     5701 acccatgcgg tagacgatcc cgcttcctac acctgcaact aaacccgcaa gtagagaaac
     5761 gatcagtaga tcatgctgta aattgatttc tagtggaacc ctttgccaga tccataaaaa
     5821 gaatgacaaa gaaagtgtac ctagaacagt ataggccaag gaacgtttac cgaggatctt
     5881 tcctcctagt aaaatcaatg ggatgttgat taataaagtc gtataagctg gatcaatacc
     5941 aaataaagct cttaagatca aggtgatccc tgagactccc ccttctgcta aatgattagc
     6001 gatgttcaag taaacaagac caaatgcata aatacttgtt cctataatga taaaaagaac
     6061 ttcttttact gtttttcgtt tgaccatatg aattccagct ttcaattatg tattttcaca
     6121 gaatttccat ttagaaattc cttcctaaat gtagcatgga aattcgattt tctcaattgc
     6181 tcttatctag gatttgcgtt aaagttttgg taagatagtt ttggagggaa aaagatggat
     6241 aaaaaacgtt ctttatacag tatgcagcag gaagtggatg aatatatcca gcaattcaag
     6301 gtggggtatt tctctccatt agctcagatg gctcgattaa ctgaagaagt aggtgaattg
     6361 gcaagagaag tcaaccacac ttatggagaa aaaagcaaaa aggcttctga accagttaac
     6421 tcggtagcag aagaacttgg tgatgtttta ttcgttacga tgatcatggc taattcattg
     6481 aatatcgact taactgaagt ttttgaaaaa aacatggaaa aattcaatca aagagaccat
     6541 aatcgctttg agcgtaagga cgagtaaaca agatgaagtg gtcctggcct tgaagactct
     6601 gaaacggact tgtcaaacag tcaccatgca actgagtctt tatctcagga caagaaacac
     6661 gcaggaaagg ttggaaaaat gaaattaaaa caattacctc tggaatacca aaaggcaatt
     6721 ccagttctga aaaaattaga gaatgcagga tatgaagctt attttgtagg tggaagtgtt
     6781 cgtgatatct tgctgaacca gccaatccat gatgtggata tcgcaacaag cgcatttcca
     6841 gaagaaatca aacagctttt tccaaaaacg atagatattg ggatcgaaca tggaactgtg
     6901 ttggtattag aaaatgatga acaatatgag atcaccacat ttcggacgga atcaacttat
     6961 caggactacc gtagaccaga tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa
     7021 agaagagatt ttacgatcaa tgcgtttgct ttgaaagaag atggagaaat catcgatttg
     7081 ttcgatggtc tgcaagatct agaagaacag atcttgcgag ctgtaggcaa tcctcatgag
     7141 cggttccatg aagatgcctt acgaatgatg cgcgggttgc gttttgtcag tcaactaggg
     7201 ttcaaattag aggaagaaac cttctacgcc atcaaagaaa accacgagct attaggtaaa
     7261 atatctgtag aacgtataaa tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc
     7321 gcgggaatcc atgcatttat tgaaacagag tgctacattt gctgtcccgg tttgaaagaa
     7381 gaaggcgaag cacttttacg ttttgctgaa ttaccatccg tcccattgaa agaagagagc
     7441 caagcatggg cattattgat cagccaactg gatctaaaag aaaatgagat tcgttccttt
     7501 ttgaaatcgt ggaagtgttc taaccaaatg attaaagacg ttcaagcact tgtccaagga
     7561 ctacgacaac gagaaactgg agctttggaa ccattcgaat tgtacttctt aggcaaaaaa
     7621 catgcactgc aagtagaaga attgatgatc tattttgacg aaacacctca gatggatgcc
     7681 gtaggagaat cctatgatcg tttacctatc aagtcattaa gcgaattagc agtagatggt
     7741 aaaatcttgc tagaagaaac tggaaaaaaa cctggtaagt gggttagtga agctttgcaa
     7801 ttagctgaaa aagccgtcgt agaacaaaga ataaaaaatg ataaaaaaca ggttttggat
     7861 tttttatcga aagaaaaatt gatttaaaaa acactagact gtatctgctc agctttgttg
     7921 gctacttaaa gtgggcagat acgtcattag aagaaaaaac ataagatgtg accacttaca
     7981 taaatttgtg ctaaactgag gtagtacaag aataaattac atcataaagg agtcttttta
     8041 atgagtaaag aaatgacagc gctaaaattt tattttagaa atggagaaac ctggacgatc
     8101 aaccgccgtc atatcggcga tctttggatc aaacaaatca caacaagttt tggacgaatc
     8161 aatggcagtg aatttgttga aatccatccg tgtgctggat ttaaaatcga aatcttccat
     8221 gaaggggacg cagttgcgac acatgatatc aacctaggcg gactggaaat gggcatgttt
     8281 aatcgcgcat tgaaatacga agatatcgag cgaatggaaa tcctttaccg aaatggcaca
     8341 cctgatcttg tgtacttccc atatctagat aaaggaacag aaggattaga caatcaatat
     8401 caatcaacaa aaatcagcga aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa
     8461 cgtgtagaag atgtatacgg ggaatttttc gaataaatcg atttttacgc aaaaatgagg
     8521 ctgtgaaaga gatgtgtctt attactactg ataggctgtc tcttccacac cgtttattca
     8581 gaataaggga ctgtgacaag atttttgtcg cagccccatt tttttgttgt gataaaagaa
     8641 caagaaaggc aggaatggga aagtaaacat ccttttgtca gaaactttta tctttcttaa
     8701 gttttacttg atatctgata ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa
     8761 tttctaacta gcaataaacg atgaaacaat gaaatatttt tcatccaaaa agaactttgc
     8821 tataatgatg gaagtttgtg tataggagat ctggaaaatg agagagagtt ttgaacaaca
     8881 aaaaaaactg ttgcatgatc gctatggcgc cctttcgatg gatgaccgcc gtcaaatact
     8941 ttgtaagttg cgtaaaagaa atatattgat gtatcgtcag ttggaacgtc tgaaacatga
     9001 tctgcttcgt ttggagtcaa aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt
     9061 tgaagtagtc gaaaccaaaa ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca
     9121 aaataaaaaa taaaggggat ggacgatgga atttgctgag ttagttattg tatttgcagt
     9181 aacgatcacc ttatcgaaca tagctagccg cattttgcca atgattccag ctccactgat
     9241 ccaaattttc cttggagtgg tattaggtct gacagagtgg ggacaatcaa tcgattttga
     9301 acctgaattg ttcttagtta tgattattgc accattgtta tttagagaag gagaaaaagc
     9361 agatatttct tcgattctca aaaatttcgg gacaattttg ttcttggcat ttggaggagt
     9421 cattttgacc cttgtcgggg taggtgcgac gttatcattc ctgttgccaa gtgtcccact
     9481 tgctgcctgc tttgcttttg gagcagcgtt aggtccgaca gatgcggtag cagtcagttc
     9541 tctttctgga cgagtgaata tccctaaaaa ggcgatgcat attttggaag gagaaggtct
     9601 tttgaatgat gcatctggtg ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg
     9661 atctttttca gcagtaaacg cggggatgag tctagtagtt tctagtattg ggggagcttt
     9721 gatcggcttt ttgcttgtct ggttcaaaca aaaaataatt catttgatcg aaaaagcatc
     9781 agcacaagat gtcacagggt atctgctgat cgaacttttg cttccttttc tagcctatgt
     9841 attggcagag gcatttgatg tatctggaat cattgctgca gtggcagcag gtattctcca
     9901 agcttctggt tttaggaaaa tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag
     9961 tacatggaca actattgctt ttacactgaa tgcattagta tttatatttt taggaatcga
    10021 attgacgcaa gtgttctcgc cagtatgggg agacggttta tatccaaacg gtcttttatt
    10081 agcgattatc gtattaatct ctgtcatgct ttttgtgatc agattcattt ctatcagtct
    10141 tttttatgta ttcaaagatg gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat
    10201 cttaacattt ggcggtgtaa agggaacagt cagtttggct acgattttta ttttgccatt
    10261 ttcaatcaat aatatgatgt tttaccagcg gtctttgctt ttgtttctga cggcgggtgt
    10321 catcttagtg actttggtca tagggatcat tgtcttgcct atgctgacgg aaacagagga
    10381 agcgaaaagc acggatttga atgctttgat gattttagaa gaggtcgttg aagttttgcg
    10441 aaaagaaata aaggaaattg atcgtaatac aaaagaattt ttagccactg aagcagtgat
    10501 agaaaattat caagaacgga tacgtgatct gtacttggaa gatttaaccg atgatgaaaa
    10561 gcaagaagta caagaaatac aagcactgat tttgtcgatc gaacgggatg gattggatga
    10621 aagttatcgt tcgggcaagc tttcttctaa tggttatcgt ttttactccc gttttctttc
    10681 tcgattcgaa cattctatca caagccagat tttgtctttt atcggctttt ggttcattgt
    10741 tgtccgtagg ttaatgcgaa tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc
    10801 tgatcgtcag acatttatta gtgatgaaga tatccaagaa atcagagaga catatcaaaa
    10861 aaatactcag ttgattattg aaagtttaga taacttgact gatatttatg atgaaacgat
    10921 ggttcaattt tttatccagc aacgcaaact tgaaggaata aaaatgaact cggggaattt
    10981 gatttcttcg tggatgatcc agcaagatag tctgtttacg aaaaaaatgc taagaggtta
    11041 ttatcttgaa agaaaaatca ttgatgaata cgaagttgct gaaaaaatca cgacgttttc
    11101 tgcgaacagc tatcgcagga atatcaattt gttagaatct tatacgatga acaaaccatc
    11161 tgataacttt tctttccgat ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa
    11221 ataaaaaggt aaaaattttc aagttttata aaaaacaaaa catatcagaa caggatcgaa
    11281 tcggtcctgt tctttttttg agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa
    11341 gttcactatt tttatgatac aatatccaag ataaggaggg aacatgttga aagaattaaa
    11401 agtaactgac ctcaaaaaaa cttatggtga aaaagatttg tttgaccaga tttctttttt
    11461 ggtccacgaa aaagaccgta tcgggttgat tgggacgaac gggacgggta aaacaagtct
    11521 cttaaatatt attgccggta tcgatagcag cgacggtgat cggcaaacag tgttttatcc
    11581 aactgattac cgtattggtt atctttctca agcagctgaa ttttccgatg aattaactgt
    11641 tctccaagca gtctttcaag gaaacagccc cttgatccaa actgttcgag catatgagga
    11701 agcattgatc gaactagggg aaaacggaga agaccctgat gtgcaaaaac gttatgctaa
    11761 agcagaagaa caaatgaata aagaagatgc ttggactaca gataccaacg caaagattat
    11821 tttgcaaaaa ttaggaatca gtgaattaga taaaaaaatc agtactttat caggtggtca
    11881 gaaaaaacga gttagtctgg cgcaagtgtt gattgatgaa cccgatttac tattactaga
    11941 cgaaccaaca aaccatttag attatgaagc gatcgagtgg ttggaaaact atttgaaaca
    12001 ataccgtggc gcactattga tggttaccca cgatcgttat tttctagata gagtagcaaa
    12061 ccgaatcttc gaactttcat ttggaaaatt atacgagtat aaaggaaatt atgaagctta
    12121 cgtcttagaa aaagcagaac gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg
    12181 gctttataaa caggaattag cttggatgag ggctggcgta caggcacgtg gcaccaagca
    12241 gcaagcacgg atcaaccgtt tcgaagatct aaaggagaac ttgtaccaag taaaccaaga
    12301 agatgattta gagctgaacc tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa
    12361 agatggcagt taccgtataa atgaccaaac actgctagaa catttagatt tattgattca
    12421 atctcgggaa agactaggta ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa
    12481 tattctagct ggcagaattc ccctggatag cggtacaatg tctatcggtg aaacggttca
    12541 tcttgcgtat tatacacaag aaaatgaaga aatggcacca gataaacgga tgattgctta
    12601 cttacaagaa gctgctgaag aagcaaaaac agcagatggc agtcaaatcg gagtagcaga
    12661 attacttgaa cgatttttat ttccaagatt tatgcatggg acactgatag gcaaactttc
    12721 aggcggagaa aaacgccggt tatttttgtt gaaactgctg attcagcaac ctaatgtttt
    12781 attattggat gaaccgacca atgatttgga catagcaaca ttgacgattt tggaagatta
    12841 ttttagaagt tttccaggtg ctgtgatcac tgtttcccat gaccgttatt tcttggacaa
    12901 ggtagcagat aagttattag tattccaagg aaacggaaaa caagaacttt attacggaaa
    12961 catgtccagt tatttactga agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc
    13021 aaaaatacag agcaaggaac ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga
    13081 atgggaaacg attgaagatg agattgcaga acttgaagaa aaaatctcat tgctccaaga
    13141 agagatgaat catcaaggag ataatttcac tcgattacaa gaattgcaaa acgacgtatc
    13201 tgaaacagaa gcacaacttg aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt
    13261 agaagattag gaggaaaaag gatggaacaa gcgtatcttg acttaggaag aaaattgttg
    13321 gaagaagggc atcgtaaaga agacagaaca ggcacaggca cattaagctt attcggatac
    13381 caaatgcggt ttgatctgca aaaagggttt cctttgttaa caacgaaacg agtacctttt
    13441 ggattgatca aaagcgaatt gctttggttt ttaaaaggag atacgaatat ccgctatcta
    13501 ttacaaaata aaaaccatat ttgggatgaa tgggcatttg aacgctacat caaaagtcca
    13561 gactataatg gaccagatat gactgatttt ggccatagat gtttgacaga tcctgaattc
    13621 aatgaagaat ataaaaagca gagcaagctt ttttgcgaga agatcctaac cgatgattcc
    13681 tttgctgaaa catacggcga tcttggacac atctacggct atcaatggcg tcactgggaa
    13741 acaaaagacg gtggctttat cgatcaaatc aaacaagtga tcgaagcaat caaaaagact
    13801 cctgattcta gaagattgat cgtttctgca tggaatccgg aagatgttcc tagcatggct
    13861 ttgcctcctt gtcatacgat gtttcaattt tatgttcaag agggacggct aagctgccaa
    13921 ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg
    13981 ttgctgactc acctaattgc acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca
    14041 ttaggagatg ctcatcttta tatcaatcat atcgaacaaa tgaaagaaca attgagccga
    14101 gaaatacgcg catttccgac tttagttttg aatcaggaaa agcagtcagt atttgatttt
    14161 gagatggaag atatccaatt agaaggatat gacccacatc cggcgatcaa agctcctatc
    14221 gctgtttaga atcagaaagg atgagaaaat gttagctgca atttgggcac aggatgaaaa
    14281 cggattgatt ggaaaagaag accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt
    14341 caaacaaatg acagaagcaa atacactagt gatgggacga aaaacattcg aagggatggg
    14401 tagccgtccg ttgccgaatc gtcagacgat tgttttgacg agagattctt cctataaagc
    14461 agaaggcgtg catgtcatgc atagtgtgga agacgtgctt gctctggaaa aagaaacaga
    14521 cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc cattttgtac
    14581 gatattatac cgtacagtca tccatcatgt attcgatgga gatgcttatt ttccacctgt
    14641 tgactggtct gattggtcac tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg
    14701 ttatccgcat caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg
    14761 cgaattgacg atataaaaaa gctatggata gactagctgt tatttagagc tgtcccatcc
    14821 atagctttat tttttttatt aagtaaagaa taaaacagag aagtacataa aacctgcacc
    14881 taacataaca aacaaatgcc aaacaacatg catatagcgg atagaattca agctataaaa
    14941 gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc aaaataacac cagtcattcc
    15001 aagagagtga taaagaggaa cagcagccac gacacagagc caccccataa aaatatagat
    15061 gatcgtagat acttttgata ctctctcccg tttatgcaac gtcaacgctt tatatataat
    15121 gccaagaatc gcaaacaacc agattaatac aaataaagtc caacctagcc atcctcggat
    15181 actcagcaaa caaaaaggag tataacttcc tgcaatcaaa agaaaaatcg aactatgatc
    15241 gaatacttgg aacacctttt ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt
    15301 taaaaataaa agaatcatca tcgatccata gatagcataa gagacaatat ggagggagga
    15361 atgcaagtgg gcacctttta ctaaaagaac cactagtccc gcgatactca atccagtccc
    15421 aattccatgg gtgactgcat taaacacttc atttaatatt tcatacgttc gattaaattt
    15481 cgttttttcc atgaaaacgg ctcctttact aggtgtaatt aatgaaaaat cattatttgt
    15541 ctgttatact aaaaatagaa attgtcttac atgtagttta acatgaataa ggcatcttgt
    15601 taaagaaaga gagagagaaa ataatgacaa atgtaaaaat cgtaacagat tcttcatgta
    15661 cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt catgccgcta tccatcatga
    15721 tcgacggaac agtatatccc gatgatgacc aactagacgg cgaaagattt atggaaatga
    15781 tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc aattggagaa ttcatctcga
    15841 tgtatgatga gctaggagaa gacggcagtg aagtgatttc gatccatatg acaaaaggct
    15901 tgagtggaac agtagaagca gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg
    15961 ttatcgacag tgataccact gaccaaggat tatcttttca ggtaattcga gcagctgaat
    16021 tagcaaaaga aggtaaaaca gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca
    16081 atacaaaatt atacatcgga atatccacac tagataactt ggtaaaaggt ggaagaatca
    16141 gccgaacaac tggtttgctg tctagtgttt tgaacatacg cgtggtgatg aatttcgccc
    16201 attctgaatt aatccctgta actaaggggc gaggaaagaa aacgttcgat aaatggttta
    16261 ataatttgaa aaatgaactg aaagaattgc caaatgtgcg acaaatcggt atttcacatg
    16321 cggatgcaaa agaattggcg caagaattca aagagggttt gcaagaatta ttcccggaca
    16381 tgcatatccc tgtactgcat acgaatccgg ttatcgctac gcatactggt aaagacgcgt
    16441 ttgcaatcat gtattatgta gattagtcga aaagaggata agccctcagc ctgaaaggct
    16501 tgcagcttat tctctttttc tttataaaat taggaaaata attgttttct tttttcataa
    16561 agactacctt tttgataaaa tttttgataa cattagaacc aatcgaatac aagaggtgaa
    16621 aaaatgaaat taacgaaacg acatttgatc ctcgttttgt tcccattatt aattgcgatc
    16681 atcatctatg caggtttagc tgcttctata ccgaaagctg gcccactaat gaaaccagta
    16741 actactgtga gagaagatcc aaataaaaaa gaaaaacttc actacatagc tatcggagat
    16801 tcgctgactg aaggagtagg agatcaaacc aagcaaggag gattcgttcc ccttgtcgca
    16861 aatgatctaa aagaccgtta taatttgacc gcggttgaaa cagaaaacta tggtgtaaat
    16921 ggtgaacgaa gtgaccagat cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat
    16981 atcaaaacag ctgattttat cacactgact gttggcggta atgatttgat gaaagttatc
    17041 caaaataatt tattcgggtt aacggtcaat tcctttaaaa agccactgaa aaactatcag
    17101 gaaaatgtca cagaactatt ggaagaattt cgatccctca ataaaaccgc acctatttac
    17161 attttaggca tctacaatcc attttatttg aactttcctg agatcactga catgcagaca
    17221 attgttaaca attggaacaa aggaacagaa acggtgacaa aaaaaatgcc gaactgctac
    17281 tttatcccaa tcaatgattt gctttatcaa ggattgaatc aggaagtagg tgtcacagaa
    17341 gaaggaacag atcaaacaac tagcacgaca gaaaatctaa atggcatcaa aaataatgtg
    17401 ctctatgaag aagatcattt ccatccaaat aatttaggtt atcaattgat ggcaaatgca
    17461 gtgagagatc agctgatcga gacacaaaac caatggttag agaaggagga tgatcaataa
    17521 acatgaaaac aagatctgac gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat
    17581 tcgccttcct tatccttctt ggggtagtac ttggttcgac cgtattttta ggcagccgta
    17641 tttttgctaa tcgggaacca gaactcccag aaataccagc tcttacggaa agacaaggag
    17701 atcctgtctt aacgatcaat tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc
    17761 ttagtgagta tcaaaaagat agtgatattg aatataaatt ttatttggaa aatgaagcac
    17821 tactcaatgg tactttcgaa gtactaggtt ttcctatcga tttctattta tattttgatc
    17881 cgtatgtgat ggaaaacgga aatgtccaac taaaagctag aagtctttct atcggaacat
    17941 tgagtttgcc aatcaaagat gtgatgaata tgatcaagag aaactataaa ttaccagatt
    18001 ggatcgaagt caatacagat gatctgacta tcatgcttag gttggatcaa ttccgaatgc
    18061 aaaacgggat gtatattaaa gcagataaaa tcgacttagt aaatgatgac attcggttta
    18121 gtctatattt gccaaaagat acctctgatg cttcaaacaa ctcaaatacc aacaaaacta
    18181 gcaccagcag tactgaaagc aagacaaaag aaaataaaaa atcatcggat gagtagtatt
    18241 agagggggaa tcataatgga acgagcaata tttgcaggag gctgcttctg gtgtatgatc
    18301 cagcctttcg acacgcttcc tggtatccat acagtgatgt caggatatac tggagggcat
    18361 gtaccagatc cgacttatga acaagtaaaa agccaaacga ctggacatac agaagcagtg
    18421 gagattatct atgatccaac attgatttca tatgaacaat tagtagaact ttactggcag
    18481 caaactgatc caactgacgc tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg
    18541 gtgatctttt atttgacaga agaacagcgg atcattgctg aaaaaagtaa gaaacagtta
    18601 gctgaaagtg gacgtttcga acagccaatc gttacaaaaa tcgaaccagc ccaaccattt
    18661 tatctagcag aagaagacca tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga
    18721 aaccatgcac gccgggctgc ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa
    18781 gcttttttca ttatttaatg acactgcgag gcggtggcac acatgatgct ttatctgttt
    18841 tcgctacaga agccggtaaa gacatccaat ttcctaagca cagctctagc tacgaagaaa
    18901 tttctaatta tctggaactg aatgtcgact atttgccgtc catggatatc tttgacgaag
    18961 catgggaaaa atatttagaa aataatcatg acatataaga gatagttttg tactttatga
    19021 tcaaaaaagt tttttgtcca atagcctaat tgaaagaatt atttttattt cagctatcta
    19081 gtcgcatttt tttgatattt ttcttataat aaagaaagaa tgaaaaaaag agggatggat
    19141 tgatagagat tgacaaaaaa acaacgctat attaatcgct tctggcttgt attcaaatac
    19201 ttacttgttt ctgtattcct tgcgttcatg ttgcggggat ttttattcat cccggtacca
    19261 gtggaaggca attcaatgga aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa
    19321 ttttcggaga tcagacgatt tgatattgtt gtttttcaat tagcagatgg cacgatttat
    19381 atcaaacgag taatcggtct tccaggagaa aatgtcagct accaaaatga tcaattgaag
    19441 atcaatggga aagtagttaa ggaaccttat ctaactaaaa acctgaaatc agatcatgca
    19501 aatgcatcct atacgacgga ctttacacta caagagctaa cgggacaatc caagcttccg
    19561 gaagataatt attttgtact tggagataat cgaagagttt caaaagatag tcgttcgttt
    19621 ggcacgatca ataaaactga tattctaggt aaagcaagat ttgtctatta tccacttgat
    19681 gaaataaaat ggatccagtg agaaacaata aagcgtgcag tctgttgaga ctgcacgctt
    19741 tattgtttaa tccaaaatca gtttactacc ttagtacgta aaatcaaaca gctcgtttaa
    19801 acacttttct agcctctaaa gttttgataa atgaataaac aaccattatt tgaataggga
    19861 tttggatcat attcctgatg aatctaccta gaaaaattac tttgaaatct gcaccaaaca
    19921 aaacagacat cagccaagaa ttgataaaaa ggctgactgg gatcataatc aataattggg
    19981 cagcgatgac ggaaggcagg tttatctttt tatgatataa aaataagcca ttaataaatc
    20041 caccaaggaa agccgtgata agaaaagcag cttgaaacgt tcctgaacca aatatcgtga
    20101 aatttaaagt gttcgacaac atttcaagca aaccagccca gatcggtcca tatagataac
    20161 ccataagcgc catacttaaa aatccgaatg tgatttgaaa ataagcagga ccaatcgcta
    20221 tcttgtataa gatgacatca agggctaata aaagagcaga ggtcgacaac aatcttgcat
    20281 tcatttcggg caatctaaaa acgtcttttg caatcaaagt gttcaaaata ttctctcctt
    20341 tgtcagttaa taataaaagg ataccataaa atcaccaatg tgtcatgaca cattggtgat
    20401 ttacatacac tggcatctaa caattaaaaa aagggtgaga agactgcttt tataaactgt
    20461 aaaaaagaga ttgtacgaat aaaaaaaata gaggcggaga atacttatcg tatcatccgc
    20521 ctttgttttt ttgttataaa aaacgtcaga ttagttatct gcttacatct tcatccatac
    20581 gggttctaat gtatttccgt ctagatcttg gacttctaat gaaaacattt cttcttcagg
    20641 cattcccata tctacttcgt ggaaagtacc accattttct tttgcaattt gagcaaattt
    20701 ttttacttca tccgcatttt caacactaaa cgaaatcaaa gcaccacttg tttttttatt
    20761 atcagctatt tctttatctt ttataaattt tttataaaaa tcatgagaga gaagcattat
    20821 ccaaaaagtg tcatcccata ccattgaacc agcttcttca gtagaaaatt cttcattttt
    20881 tttaaaaccc agcttttcat aaaaagcaac ggaacgattg acatcagcaa caggaaaatt
    20941 aacaaaaacc atactattca tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc
    21001 ttatttagga aacgctgtca aaaatttaga ttcatatata gaaaccaaaa taaaaatttg
    21061 catcatcaaa aaagtgaaga tgtgatagaa tgaggactgg ttcactttca cttaactaat
    21121 agatggaatt tctttataat aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa
    21181 agccggggaa tattccggta tgaaaggaat ggagagaaca atgaccatcc aatggtttcc
    21241 gggacatatg gcaaaagccc gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt
    21301 atttgaattg gtagacgcac gattaccttt atcttctcgt aatccaatgc tagatcagat
    21361 cattcagcaa aaacctcgcc ttgttttatt gaataaagcg gatctggcag atccacaaca
    21421 gacaaagctg tggcaagaat attttcgtaa acaaggccat cttgcattag ctatcacggc
    21481 acaagaaagt aaaggaatta aaggtctagt acctgcggct aaagaagcat tgaaagaaaa
    21541 acgggcaaga gatgctgctc gggggatcaa gcctcgcgct attcgggcaa tggtcttagg
    21601 tattccaaat gttgggaaat ctacgttgat gaatcgacta gtaggcaaga aaattgcaca
    21661 gacaggaaat aagcctggcg taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt
    21721 ggaattgtta gacacaccag ggattttatg gcccaaattt gaagatgaag aaatcggcaa
    21781 aaaactagcg ctgacaggcg cgatcaaaga ccagcttgtc catttagatg atttagcgat
    21841 ttacggatta gacttttttg cccgatatta ttcaggaaga atcagcgatc gttatggttt
    21901 atcaatcgag caagaagaaa aattggcacc ggaattactg atggatatta cagcaaaacg
    21961 tgggtataaa gaagactatg atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg
    22021 aaaacttgga cgatacacac tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg
    22081 aatccatcca aagtatcaaa gaacgattga aaagcataca gagaatagat gatcctacac
    22141 tagaatcatt aagaaatgat ccacgcaaag gcgtgcagca agcactggct tcttttgaac
    22201 gacgattgaa aaaggaagca ttaattaagg aaaaatatga agaaatgttt tgctttgaaa
    22261 agcaagggta cgcacaaggt caccaatgga ttgccggcat tgatgaagta ggaagaggac
    22321 cattagctgg acctgtcgtg gcagctgcag ttatcttgcc gaaaaatgag atcatattag
    22381 ggctgaatga ctccaaacaa ttatcagaga aaaagcgaga acttctttat caacaagttc
    22441 aagaaaaagc cctggcaatc gggatcggga tcgttgatca gcagacaatc gatgatgtga
    22501 atatttatga agcaagtaaa ttagcgatga tccaagcagt agaaaaattg tccattgtac
    22561 caagctattt gttgatcgat gcgatgacct tggatttgcc tattcagcag gaaaaaataa
    22621 tcaaagggga tgcacgatct gtgtcgattg ctgctgcaag tatcattgct aaagtatatc
    22681 gagaccaatt gatgaaagaa tatgacgtgc tttatcctgg atatggtttc tcgcataatg
    22741 caggttatgg aacaaaggaa cacttagaag gacttgaacg acaaggaatt acgccaatcc
    22801 atcgtaaaag ttttgcacct gtaaaaaaat atttacaata aaaaacagcc gcttttacgt
    22861 atcttattag aaagagagac gaaaggcggt tttatgatgt atcaaataga agaaaattta
    22921 ttgaaacttg cttatacaaa aggaatcagt agtcaaggaa aatgggcact tgctaactgg
    22981 atgatgcagt atccttataa agaaatcgga tttgaagaca tcattaagat aggtaaaata
    23041 accaaacacc gggagctgtt tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa
    23101 atccaatcga aacagtcgtt tatcacttgc tttgatccca aatatccccc tcagctgctt
    23161 cacttgacct atccgccaat tgtactgttt tataatggtg atctatcttt actttcttgt
    23221 aacatgttaa gcgttgttgg tggaagacac acttctgctt tagccaaaaa aacagtttat
    23281 tatttactaa aaccagtgat agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg
    23341 atagatacat acggtcacca agcagcaatc tctcatggtg gacggacaat cgcagtcatt
    23401 ggaacaggga tagagcaagt ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca
    23461 aatgatcatc ttcttttgag tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt
    23521 ccaatgagaa atcggattat cgcagctttg tctaaaggaa catgtgtctt tgaagccaag
    23581 aaaaaaagcg gctctctgat aacagcacag caagcattgg atcttggaag aacagttttt
    23641 tctgttccag gaaatatttt aacagaacat tctaccggat gtcatcattt gatccaagac
    23701 ggtgcagttt gcacggtttc gggtgaagat atcttggcag aatttaaaga ataacaccat
    23761 acttttcatt tgcttttctt gacaaacgtt ttctgaatgg atatgattag ctacgatttg
    23821 tatgaatcct cttttaatag taggaacacg acaatggaaa ggagtccatt cagtaatggc
    23881 gtataaatat ttagtgattg tagaatcacc tgcaaaagcc aagacaattg agaaatatct
    23941 tggacgaaac tataaagtaa tggccagtgt tggccatata agagatttac cgaaaagtaa
    24001 aatgggtatc gattttgaaa acaattatga accccattat atttctatac gcggaaaagg
    24061 cgatgtcatc aaaagcctga aagccgcagc aaaaaaagct caaaaagttt acttggcaag
    24121 tgacccggat agagaaggag aagcgattgc ttggcattta gcgtacctac ttgggttgga
    24181 tctgaaagaa aaaaatcggg tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc
    24241 ttttaaggaa ccaagaacga tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac
    24301 cttagacaga atcgttggtt attcgatcag tcctattctc tggcgtaagg tcaagaaagg
    24361 gttaagtgca ggacgtgtcc aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga
    24421 gatccgagaa tttgttccag aagaatattg gagcatcgac ggtaatttta aaaaagctcg
    24481 caagaaattc aaagcaaatt tctggggaat cgacggtaag aaaaagaaat taccagatgc
    24541 acaaagtgta aaagaagtca ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt
    24601 agaaaagaaa gagcgcaaaa gaaaccctgc gaatcctttc actacaagta gcctgcaaca
    24661 agaagctgcc cgtaagttaa actttcgaac aagaaaaacc atgatggtag cacagcaatt
    24721 atatgaagga atcactttgg gtaaacaagg aacagtcggc ttgattacat acatgcgtac
    24781 tgattctacc cggattgccg atacagcaaa agcagaggcc gcagcattta ttgaagatac
    24841 gtatggaaaa gaattttcta gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca
    24901 agatgcccat gaagctgtgc gtccaacgag tgtgcttcgt acaccagatg aactcaaaca
    24961 atatttagat aaagatcaat tgaaacttta tacattgatc tggtcacgtt ttgtggctag
    25021 tcagatgaca ccagctattt tagatacaat gaaagtgaca ttagaacaaa atggtgtcat
    25081 cttcattgcg aacggttcaa aaatcaaatt caaaggattc atgcaagtgt atgtggaagg
    25141 tagagatgac ggcaaagaag agaaagaaaa tattcttcca gaacttgaag aaggcgatgt
    25201 agtccaatct gttgatattg aaccaaaaca acattttact caaccaccag cacgttttag
    25261 tgaggctacg ttgattcgtg cactagaaga aaacggggta gggcgacctt ccacttatgc
    25321 accaacactt gatacgatcc aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga
    25381 gccgactgaa ctaggagaaa ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt
    25441 cgatatccat ttcacagcag aaatggaagg agatttggat aaaattgaag aaggcacgga
    25501 agcgtgggtc aaagtggttg atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc
    25561 ggaagaaaaa atcgaaaaaa ttcaaatcaa agatgaacca gcaggattcg attgtgatgt
    25621 ctgtggacat ccgatggtga taaaattagg gaaatatggg aagttttatg cttgcagtaa
    25681 ttttcctgat tgtcggaata cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt
    25741 atgtcatgaa ggacaagtca ttgaacgaaa atctaagaaa aacagactat tctatggttg
    25801 ttcccgtttc ccagaatgtg aattcacttc ttgggataaa ccagtgggca gaaattgtcc
    25861 aaaatgtgac ggatatctgg tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt
    25921 aaatggagat tacgaagaag acatccaaaa ataatagaaa agaggttctt tcattgaaaa
    25981 tggtaaatgt tgttggagca gggcttgccg gaagtgaagc tgcttggcag atcgcacaag
    26041 caggagtccc tgtccgttta tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca
    26101 caagtaattt tgctgaacta gtatgttcca attcattaag aggaaacagt ttggctaatg
    26161 cagtagggtt attgaaagaa gaaatgcgcc gattgaattc ggtagtaatc acttcggcag
    26221 atgaaacagc agtgccagct ggtggtgcct tagctgttga ccgcgattct ttttctgaaa
    26281 caattacccg aaaagtcaaa gagcatccat tagtaacagt gataaacgaa gaaattactg
    26341 aaataccgga aggaatcacc gtagtagcaa caggaccttt gacttctcat ccattagcag
    26401 aaagcatcaa agcattcaat ggttcagatg gtttttattt ctatgatgca gctgcaccaa
    26461 tcgtagacaa agctacgatc gatatggata aagtatattt gaagtcccgc tatgacaaag
    26521 gagaagcagc ttacttgaac tgtccgatga cagaagagga attcaaagct ttccatgagg
    26581 cactaatcaa tgccgaagtg gcagaactta aatcttttga gaaagaaaaa tattttgaag
    26641 gatgtatgcc aattgaagta atggcacagc gaggattcaa aacgatgctt ttcggaccta
    26701 tgaaaccagt tggattggaa gatcctaaaa ctggcaaacg tccttatgct gttatccagc
    26761 tgcgtcaaga caatgctgca gcttctttat acaacatcgt gggctttcag actcacttga
    26821 aatggggcga gcaaaaacgt gttttccgga tgattcctgg gttagaaaat gctgaattcg
    26881 ttcgctatgg tgtgatgcac cgaaacagct ttatgaattc gccagaattg ctgaaaccga
    26941 cttaccaatc aacgaaacgc gaagatctgt tctttgcagg tcagatgact ggtgtagaag
    27001 gctatgttga aagtgcagcg agcggactac ttgctggaat caacgccgca cgcttagcaa
    27061 aagaaatgga accagtcgag tttccacaag aaacagctat tggaagtatg gcttactaca
    27121 ttactcatgc ggaaggaaaa catttccaac cgatgaatgc caactatggt ctgtttcctg
    27181 aactgccaga gcgcattcga gacaaaaaag caagatatga agcaattgca caacgagctt
    27241 taaaagctaa tgaggcaata aaaacagaat tagaaaaaac tgcaatccca aatgagtaag
    27301 gaaactccat gaagagggtg agacggatag tctcgctctt tttcttgcgt ttttcttaag
    27361 aatttctcaa acaaaatgtg aggaattgcg ccaattgtga acaaacttag aaatattggt
    27421 taaattctga caatttattt gtctttttat aaacgctgtg ctaaattatt ctctgggaca
    27481 aaaggaaggt gagaaaatga cagaagccac gtgggaacaa aaatttttgc gttatttgat
    27541 tgtcgaaaga ggatattcag aaaaaacgaa agaagcatat gaagaagaca ttctccattt
    27601 caaacatttt ttggaggaat ctggagacgc tgatctgctg aaggcggaat attttacggt
    27661 tcgtgtctat ttgagtgcat tagctgatca tcgtcctagc tatagcagaa attctatcag
    27721 tcgcaagata gccagcctac gttcgtttta tcagtatctt ctcaaagaag aagtgataaa
    27781 agaaaatcca ttttcttacg ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt
    27841 ttatgaaaat gaaatgcaag cactttttga cagcgtagca ggagatagtc ctttggattt
    27901 gaggaaccgc gctttactgg aagtccttta cgggtcaggc atccgtttaa gtgagtgtag
    27961 cagtttgtcg gtatcagaca ttgattttga ttcagaggtc atgttgatcc atggtaaagg
    28021 gaacaaagag agatatgctc ctctaggttc ttttgctcaa gatgctctgc aagaatattt
    28081 cagggaaggc cgccaggtat tgatgacaaa atatcacgaa gagcatgata tcgtttttgt
    28141 gaatcatcat ggaaaaccaa tcacgcctac tggaattgaa tatgtcttga atcaaatgat
    28201 caaaaaaagc agtttggata gtgatatcca tcctcacatg ctgcgccata catttgctac
    28261 tcatttatta aataatgggg cagatttgcg gacagtccaa gaattgctag gacatgccaa
    28321 tttatcgact acccagatct atgcccatgt aacaaaagaa agtttacaaa aaaactatcg
    28381 ttctttccat ccaagagcgt aaaaataagg aaaagaacaa atttaggagg aaacaaacat
    28441 ggtcgaatca ccatttcatt caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc
    28501 tatggctgga gatggccaag taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa
    28561 gaaagtacga cgtatctaca atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga
    28621 tgcgtttact ttagaagaaa aatttgaagg caaactaaac gaatataacg ggaatctaca
    28681 acgcgcagct gtcgaattag ctcaagaatg gcggacacag caatctatgc aaaaacttga
    28741 agcgatgctg atcgtaatga atgataaaga aatgcttctt gtttcaggta caggagaagt
    28801 tatcacacca gatgatggta tcttagctat cggatcagga gggaattttg cgttggctgc
    28861 agcccgagca atgaaaaact atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa
    28921 tgcgttaaat attgccgcag atatctgtat ctttactaat cataatatta tcgtagaaga
    28981 actgtaagag gtgagtagat atgtatgaat taaaacaaac accaagacaa atcgtaagtg
    29041 aattagatga atatatcgta ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc
    29101 gtaaccgcta tcgccgtttg cagttagatg aaaaaatgca acaagatatt acaccaaaaa
    29161 atatgctgat gatcggacct acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa
    29221 aaatcgtcaa tgcaccattt gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg
    29281 tcggaagaga tgtagaatca atggttcggg atttagtaga aaatgcgatc caaatcgtag
    29341 aaaaagagca gtacagccgt gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg
    29401 taaaaatctt ggtacctggt atcaaaaaag aacagaaaca atccactaat caatttgaac
    29461 aaatgatgaa tctttttaat gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga
    29521 cagaagatat tcgaatcaat cgccgtacag tcttggaaca actacaaaag ggtttactgg
    29581 atagtcgtga agtaaccatt gaagttgaag accaaaagaa acctgcacca atgatgaaca
    29641 atggattgga gcaaatgggg atcgacctaa atgaaactct aggtgctttg acaccgaaga
    29701 aaaaagtgga acggacggta acagtcaaag aagctttaga gttattgatc aaggaagaat
    29761 cagcaaaatt agtcaaggat gctgatatcc atagtgaggc aatcaaatta gcggaagaaa
    29821 gtggaattat ttttatcgat gaaatcgata agatcacttc taaatcacag caatctggag
    29881 aagtatccag agaaggcgtc caaagagaca tattgccaat tgtcgaaggt tcacaagtaa
    29941 acacaaaata cgggatcatc caaacagatc acgtcctatt cattgcttca ggagctttcc
    30001 atttgtctaa accaagtgat ctaattccag aacttcaagg acgcttcccg atacgggtag
    30061 aattagatga tttgacagct gaagattttg tgaagattct gaccgaacca aataatgctt
    30121 tagtaaaaca atacgtcgcg ttgatcggta cagaaaatgt gtcagttacg tttacaaaag
    30181 aagcaattga acggatcgct caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg
    30241 gcgcacgcag actgcatacc attttggaaa aactactaga agatctatta tttgaagcac
    30301 cagatatgca aatgggagaa ataacgatca cagaagcata tgtggatgac aaacttggct
    30361 caattgcggc aaatgaagat ttaagtcgct atattttata aaaaatcgga ggacgacaat
    30421 gacagacttg ctaacgaaaa cccgcaagat aaacacactt ttgcagcaaa agaatacttt
    30481 tgacatgcaa gcagatttgc cttacgataa aatggctgtc acactaggtg gtattcttga
    30541 cagcaacacc tatatcatag gaagtgacgg agtattacta ggttataatg aacgtcacga
    30601 tgtgaacaat gaacgaatca aaacaatgtt tgttgagaaa caatttcctg aatcttatac
    30661 tgatacagtg gcacagttga tgaaaactga agctaatatc ccaattacta gtgatattac
    30721 tgcatttccg gtagagtcaa gagagaaata tccatttggt ctgacgactg tcgttccgat
    30781 ctttggagct ggcgaacgtt tgggtacgat tatcttatct cgtatggaac aatcgtttgc
    30841 agatgacgat ctagtcttag cagaatatgg agctacggtt gtcggtatgc agatccttta
    30901 tcagaagtct cgaagcatcg aagcagatgt acggagtgca acggctgtcc aaatggcaat
    30961 caacacatta tcttatagcg aattgaaggc tgtacaagct atctttgaag cattggatgg
    31021 agatgaaggt cgtctgactg cttctaatat tgccgatagt atcggcatca ctcgttctgt
    31081 gatcgtcaat gctttacgaa aattggaatc agctggaatc attgaatctc gttcgttagg
    31141 catgaaggga acttatttaa aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca
    31201 cagttattag aagtaagggg actggggcag atagaaatgt ttgttccagc cttcccattt
    31261 taaaaagtaa gtcgaagttg gaggaatggc aatgactgtt attattcaaa atgaccagct
    31321 gatcgccgaa atcgctgaac acggtgcgga attgatcagt ctgaaatcaa aagaacatgg
    31381 tttagagtat atctggcaag gtgatccagc gtattggggc agacatgcac cggttttatt
    31441 cccttttgtt ggacgtttaa aagatgatca gtatgtttat caagggaaaa catatcaaat
    31501 ggggcaacac ggatttgctc gggatatgga ttttgaagtc attgaacatg gacaagaaat
    31561 ggctagtttc atcttaagga gtacgccgga gacattagaa aagtatccat ttgattttga
    31621 attagtgatc agctatgaat taggaggaga cggcatcacg gttcattatc aagtggcaaa
    31681 taccggtgaa aaagaaatgt tcttttctat cggtggacat cctgctttca atgtacctct
    31741 tgaagaagga ttgtcatttg aagatttcta tctggcattt tctccattga aatcaagatt
    31801 acgcctacca ttgaaaggcc cattcatcga tatggatcaa aagacgttag gtcagacaaa
    31861 tacgaatctg gctttgaatc gtgaactttt tgagcaagat gccttggttt ttgaaacaag
    31921 gggtctaaat gcgtacgcca ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta
    31981 taaagatatg ccttatgtag ggatctggtc tccttatcca aaagaagcgc ctttcgtttg
    32041 tatcgaaccg tggtgcggaa tcgctgacac ggtgaacagt agtggaaact tgatggaaaa
    32101 agtagggatc aatcaactag gtgcccatga aatcttcaag acaaaatatt ccatcacagt
    32161 gaagtaagag gttgtgaccg agaagttagc agggaacacc gtttaccaag gtgtgtaaaa
    32221 aagctatcct gtaagtaata agggagtgtg acatcgattt gtcacactcc cgttttatct
    32281 ctttggaatg aaaaagtatc aggatttttt cttgctgcta ttcaaaccaa acgggattcg
    32341 actttcagta tggtttctga tacgcttgat attgtcttta tgccgataaa agataaagga
    32401 agaaactaaa aaggcaatga tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc
    32461 tacataagga aggacgatcg tagataaagt gatcaatact gcactgatca tactgctcaa
    32521 actcaccata cttgtacaaa ataaactaac gataaagatg atacaagaat aaacaaaaaa
    32581 tacaggatta aatcctaaaa gcattcctgc acttgtcgca acagcttttc ctcctttgaa
    32641 cccagcaaaa atcggaaatg tatggccaag aatggcgcat accccaaacc aaagagggtt
    32701 aatcccagaa aggtggaaga tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc
    32761 cataagcagg acaacaatcc cggctgtttt gcctaataca cgaaatgtat tggtcgttcc
    32821 agtattaccg ctcccgtgtt gtcggatatc ttttttgaaa aacaatttgc caatccagac
    32881 accggaagga atagaaccta aaagatatgc taaaataagt aataagatca atttcatttt
    32941 ttaccctctt tcaagttgaa aactatcctt tattttagca tgaataggta acaggaacaa
    33001 ttgaaaaaat gaaagataca agtataatag atggaataaa aagaaaagca aggggtggca
    33061 gaaatgtctg taaaaaatcc aacacaagaa acgattttca actatttgga agaagcaaaa
    33121 aatattgccg tcgtaggctt gagcaataaa ccagaacgaa caagttacat ggttgcacag
    33181 gtattgcaag acaatggata taaaatcata ccagtaaatc cattattggc tggtgaagaa
    33241 attctaggcg agaaagtcta tgcgaatttg acagaaatcg atgatccaat cgatatcgta
    33301 gatgtattta gacggagtga atttttacct gaaatagcag gtgatttttt gaaaactgat
    33361 gctaaagtgt tctgggcaca attagggatt gaaaacaaag aagcagctga gatgctaaga
    33421 aaagcaggac gtacagatat cgtaatggat cgttgtatta agattgaact tggtaagttg
    33481 aaagaaaacc aaaaataaaa atgaccacaa agaagagatg atcgatagag gggaatgtcg
    33541 aaaaagcggt taggagataa ttaaaattga tttctcgtct accctctttt ttttcacgac
    33601 cttttctagt atgataatcg tggtgcggtt ttgccgcaaa gtattattga aggagcgttt
    33661 gccttggcaa aaaaagtaaa caacgaatac aatgactcgt ccatccaagt attggaagga
    33721 ttagaggcag ttagaaaaag accagggatg tacattggat ccactgacag ccgaggcttg
    33781 catcatttag tctatgaaat cgtcgataat gcagttgacg aagcattatc aggttatggc
    33841 agtgagattg atgtgaccat ccatgaagac aatagtataa ctgttgcaga tagcggacgt
    33901 gggatgcctg taggaatgca tgcttccgga ataccaacag ttgaagtgat ttttactgtg
    33961 ctgcatgctg gcggaaagtt tggtcaagga gggtacaaaa cttctggtgg actgcacggt
    34021 gtaggagcca gtgtggtaaa tgccttatcg aaatggctaa ttgtaacgat cgttagagat
    34081 ggcgtagagt atcagcagaa atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag
    34141 ataggaaaaa caaaaaaagc aaacgggacg actgtccatt ttttaccaga tgatacaata
    34201 ttcagtacaa cgaaattttc atatgaaatt ttagccgaac ggttaagaga atctgccttt
    34261 cttctaaaag gcgttaaaat ctctctttct gatcttcgtg gagaagaacc agtcaaggaa
    34321 atttttcatt acgaagaagg aatcaaagaa tttgttgatt atctgaatga agaaaaagat
    34381 acattgacac cagttgttta tttttcagga gaaaaagaag gtatcgaagt agaggtcgct
    34441 tatcaataca atgacggtta ttcagaaaat gttttatctt ttgtcaataa cgttcggaca
    34501 aaagatgggg gaacccatga agctggtatg aaagcggcga tgacgaaatc ttataatgaa
    34561 tatgcgagaa aagttggttt gctgaaagaa cgggacaaga atctggaagg tagtgatttc
    34621 agagaagggt tagctgctgt actttctatc cgtgtacctg aaaatcttct gcaatttgaa
    34681 gggcaaacaa aagaaaaact tggtacgcca gttgctcgta ccgtagtaga taacgtcatc
    34741 agtgaacaaa tgggctttta tttgcaggaa aatagcgaaa tgagccagat gttggtccgt
    34801 aaagcaatca aagccagaga agcgagagaa gcagcacgta aagccagaga agaaagccga
    34861 aatgggaaga agcgtaaaaa aggcgaatct cttctttcag gaaaattgac acccgcacaa
    34921 tcccgaaacc ctaagaaaaa tgaattgtat ttagtcgaag gggattctgc tggcggatct
    34981 gctaaacaag gaagagacag aaagttccaa gcaattctac ctttacgagg aaaagtcatc
    35041 aataccgaaa aagctaaaat gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc
    35101 tatacgattg gtgcaggagt cggtcctgag ttttctatcg aagactgtaa ttacgacaaa
    35161 gtaatcatca tgaccgatgc ggatacagac ggcgcccata ttcaagtatt actgcttact
    35221 ttcttttatc gttatatgaa accgctgatt gaagctggga aagtatatat cgcgttaccg
    35281 cctttatata aagtatccaa aggacaagga aagaagcaag tcattgaata tgcgtggaca
    35341 gatgacgaat tggcagcaat gattaaaaaa gtcggcaaag gatatatgct gcagcgatat
    35401 aaaggacttg gagagatgaa tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc
    35461 cgtacgttga tccgcgtccg tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca
    35521 ctgatgggtg acaaagtaga acctcgaaga aaatggatcg aaaatcacgt gcaatttacg
    35581 ctcgaagaag atggcagtat cttagataaa aaagaggata ctgaaatctc tccttcagtt
    35641 tctaatgatt tattagatga agaacgagca gataaaaatg aaaacaatca gttatttgaa
    35701 gtagaatagg aggtcacctg atggaaaatc gacaagaagt acaagaatta actttagaag
    35761 aagtgatggg tgaccgcttc ggacgttatt ccaaatatat tatccaagaa cgtgcactgc
    35821 ctgatatccg tgatggattg aagccagtgc aacgccggat attatttgcc atgaacaaag
    35881 atggcaatac gtatgacaaa ggattccgta aatcagcaaa atctgtcgga aatatcatgg
    35941 ggaattatca tcctcatggt gacatcagta tttatgaagc gatggtccgc atgagccaag
    36001 actggaagtt gcgggaagta ctgatcgaaa tgcacgggaa taacgggagt atggacggtg
    36061 atccacctgc tgcgatgcgt tatactgaag ccagattatc caagttaagt ggagagatgc
    36121 tagcagatat cgagaaagaa acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag
    36181 aaccaacagt ccttccagct cgatacccaa accttctcgt caatggatcg acaggtatat
    36241 ctgctggata tgcaacagaa atcccgactc ataacttagc tgaagtgatc gatggcacaa
    36301 tctatatgat cgatcatcca caagcgagcc ttgaaaaatt gatggaatat attcctggac
    36361 ctgactttcc aacaggaggg atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa
    36421 caggacgagg aaaagttatt cttcgttcga aaacaaagat agaggcaatg aagggtaata
    36481 aacagcaaat cgtgatcaca gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa
    36541 aaatggatga gatccgtttg aataagaaaa tcgatggaat agctgaagta cgagatgaaa
    36601 gtgatagaac aggtctgcaa attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa
    36661 ttttgaatta tttgttcaaa aacactgagc tacagatcaa ttacaatttc aatatggtcg
    36721 ctatcgacca tatgactccg catcaagttg gactgaaaga cattttaaga agttatatcg
    36781 agcatcgaaa gcaagtcata acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa
    36841 gacagcatat cgtagaagga ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa
    36901 cgattcgtga aagtaaagac aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat
    36961 ttacagaaga acaagcagaa gcaattgtca cattgcaatt ataccgtttg acgaacacgg
    37021 atatcacaga gcttcaaaag gaatccgaaa gcttgattgc gcaaatcaca gagttgaata
    37081 aaatcctttc aaatgacaaa gagcttttct ctgttatgaa aaaagaattg cgagaagtga
    37141 aaaaaaatta ttcttccgct cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa
    37201 ttgatacgca agtcttagtg gcacaagaag acgtaattgt cagtgtgacg cgagaaggct
    37261 atgtgaagcg aacaagcctt cgttcttata gtgcttccaa accagaagaa atcgggatgc
    37321 gagaaggcga ctacttgcta tactcaggtg aattgagtac attggaccat gttcttctga
    37381 tcacaaataa agcaaatgtg atctaccgcc ctgtccatga actacccgat ttgaaatgga
    37441 aagatgctgg tgagcacatt tctcaaacaa tctccaatct gtctgtagac gaatcgattt
    37501 tagctgtttt cccatatcaa aaaattcaag cagagaaaac atttgtattc atcagcaaga
    37561 atggattgat caagcaaaca cgaatgacag agttcgaacc atggcgcacc tataagagcc
    37621 gtccgctcag ctgtatgaag ctaaaggcgg aagaagatga actgattgct gtgtatctga
    37681 aagaagatca gccaggctta gatgttttct tagtaacgca tcaaggaatg ggtttgcgct
    37741 atcctttaac agatgttccc gttgttggag caaaggcagc tggagtgaag tctatgaatt
    37801 tgaaagatgg agataggatt atcaacgggc tgcttgtgtt agcagaaggt gatacgccga
    37861 tcgtaatcgt tactcagcga ggagctgtta aacgcatgct tgctcaagaa atcagccaaa
    37921 cttcacgtgc gaagcgggga gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta
    37981 ttatctatat gtcggaagga agatcaaaag aaataacctt gatgaatcaa aaagggcagc
    38041 agttaacgat tgacccaact gatttcccta taggtgatcg aacatcgaat ggatcatttg
    38101 cgatggatga gaaaaaaggt ggagaagtca tcgaagttat cgatgctcct gagattttca
    38161 tcgagtcgta actgttgtag tattttaatg aagagacgcc atctgtatta atacaggtgg
    38221 cgtctctttt tcattcattt ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg
    38281 ttcacaatgc ttaaatgttg gtatgtcaac gttgatttaa atatatgtaa ataaaacata
    38341 aaaattgtta aataaaaaac aagaaaaccc ttgcaaagat agggtgctgt gttatactaa
    38401 tcacgtaaaa actgttacat aaaacagaaa tttaggaggt ccatacaatg gagcaatgga
    38461 atggatttaa aggtaccaaa tggaaagaag gcgtagacac tagagacttt attcaaaata
    38521 actacactga atatcgcggt gatgacagct ttttggaagg cccaacagaa gcaacagaaa
    38581 aattatggga tgaattgcaa aagttaaatg atatccaatt cgagcgtaac ggtgtctatg
    38641 atatggacac agaaatcgta tcaactatta cttcacacga tgcaggctat ttggataaag
    38701 atttggaaaa aatcgtcggt ctacagacag acaaaccttt gaaacaagca ttcatgccgt
    38761 tcggcggaat caatatggct aacaatgcgc taaaaagcaa tggctataca ccaaacgatg
    38821 aattgacaca tattttcact gactggcgca aaacacataa tcaaggtgta tttgacgctt
    38881 atactgctga aatgcgtgct gctcgtaaaa acaaaatcat tacagggctt ccagacgctt
    38941 acggtcgcgg acgtatcatc ggtgactatc gtcgtgtggc attgtatgga atcgactatt
    39001 tgatggaaca aaagaaaaaa gatcatgata tgactggtta tcatacaatg agtgaagaag
    39061 tgattcgttt acgtgaagaa atcactgaac aatatcgtgc attagcgcaa atgaaagaaa
    39121 tggctgcgaa atatggctat gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc
    39181 aatggctata cttcggatat ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa
    39241 tcggacgtat ctcagcattc cttgatatct atatccaacg tgatttggat aatggagtaa
    39301 tcacagaaaa agaagcacaa gaattgatcg accacttagt aatgaaatta cgtatggtca
    39361 aattcgcgcg tacacctgaa tataaccaat tattctcagg attccctatc tgggctactt
    39421 tatccattgc aggtatggga ttagatggtc gttcattagt tacaaagaat gatttccgtc
    39481 tgcttcatac attaacaaat atgggacctt caccagaacc aaacttgact gttttgtatt
    39541 ctgaacacct tccagaagga ttcagaacat atgctagcag aatcgctatc gaaagttctt
    39601 cgattcaatt cgaaaatgat aatcagcttc gtgaacaatg gggttctgat gattgtgcga
    39661 ttgcttgctg tgtatcagga acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg
    39721 ctaacttggc taaaacacta ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg
    39781 cccaagttgg accagaatat cgtccaatca catcagaaat actagacttt gaagaagtga
    39841 aacataactt catgaatatg atggattggt tagcagaact atatgtcaat acgttgaaca
    39901 tcattcatta tatgcatgat aaatattctt atgaagcagc tcagctggct ttgatggatt
    39961 ctgacttgca acgcacattt gctacaggta ttgctgggat ctctcatgcg gcagacagct
    40021 tgtcagcgat caaatatgca aaagtaaaag caatccgtaa tgaaaaagga atagcagttg
    40081 attttgaagt ggatggcgac ttccctaaat acggaaatga tgatgaccgt gcagatgaat
    40141 tagtttcatg gatcttggaa tacttcatgg gacaaatcaa acgtcataaa acttaccgca
    40201 actcaaaacc aacaacttca ttattgacga ttacttcaaa tgttgtttac ggtaaaaata
    40261 caggaaatac tcctgatggc cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa
    40321 gttacaatgc ggaacaaagt gggttattag ctagcctgaa ctcaacagct aaaatgaatt
    40381 acggctgtgc attggatgga atttcaaata cccaaacaat caatccttca ggtcttggaa
    40441 aagaacaaga tacaagaatc gaaaacttgc gtaatgtctt agatggatac ttctcaaaag
    40501 gagcttatca tttgaatgtc aatgtattca gtaatgattt attgcgtgat gcaatggaaa
    40561 atccaatgaa atatccgaac ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg
    40621 acttgacaag agaacaacaa ttagatgtct tgatgagaac ttcacacgac agaatgtaaa
    40681 aagttcgtga aaaaagtttt tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc
    40741 aaaatcgaat aatcagtatg cttatttgaa agaaacgatt tacaggggca ggacttgtat
    40801 gttccgtccc tgtaaatttt acacataacg aagcggcaat tgcgaaaaat tttgttcaga
    40861 aagcttcgct tcgacagcta acttgtactt aaaatgaaat caaagaaaag atgtgaactt
    40921 aaatggaaga aaaaacaatc ggctatgttc attctatcga aactttcggt tcagtagacg
    40981 gacctggact gcgctttgtc gtgtttatgc aaggatgccg catgcgttgc caattttgtc
    41041 acaatccaga tacttggaat attggcggag ggaaagaata cacagcagat gaacttttag
    41101 ataaagcaga acgcttcaga ccttactggg gagataaagg tggtattact gttagtggtg
    41161 gagaacctct attgcagatc gattttttga tcgaactatt taaaaaagca aaagagcgga
    41221 agatgcatac tacattggat acatgcggaa aaccttttac gtatgaagaa cctttctttt
    41281 cacgttttca agaattgatg aagtatacag atttactgtt atttgacatc aaacacatcg
    41341 ataatgaaga gcataaaaaa ctaacgcatt gggacaatga aaatatttta gaaatggctc
    41401 aatatttgtc aaaaatcaac aaaccggttt ggattcgcca tgtactagtt cctgagcgaa
    41461 gtgattatga tgaatatttg atccgactag acaactttat tcaaaccttg tcaaatgtag
    41521 acagagtaga gattcttcct tatcatacga tgggaaaata caagtgggaa acacttggtt
    41581 tgaaatatcc attagaaggg atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac
    41641 gattgcttca tacagaagag tatacaaaat atttaacgcg ataggtcgca tcggaaagaa
    41701 gataagtatt gcttctttcc gtttttttgc tattattaaa taggagaaat cacaaaggag
    41761 cgaatgacat gtcaaaaatt ttagtttttg gtcaccaaaa tccagataca gatgcaatcg
    41821 gggcagccat tgcgttttca cacttgcaaa atgaattagg taaagaaaca gaagcagttg
    41881 ctctaggtac acctaatgaa gaaacacaat acgcattaga ccactttcag ctatctgcac
    41941 cacgagtagt agagtcagct aaagcagaaa atgcagatgt catgcttgtg gatcataatg
    42001 aattccaaca aagtatttca gatatcagcg aagtaaatat tttagctgta gtagaccacc
    42061 atcgtattgc aaactttgaa acagctaatc cattgtacta tcgtgcagaa ccagttggat
    42121 gtacaagtac gattatttta aaactatata aagaaaacca agtaacaatt cctaaagaaa
    42181 tcgcaggaat catgctctca gctatcattt cagatacatt gttgttcaaa tcaccaacat
    42241 gtacgcctga agatgaagca gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa
    42301 acacttatgg acttgaaatg ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg
    42361 ttcttcttga tttggatgca aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc
    42421 aagtcaatac agtggacctt aatgaagtga tggatcgaca agcagagcta gaagcagcaa
    42481 tggaagatga aatgaaagca aatggctacg atctgttcgt attagttgtc acaaacatct
    42541 tagacagtga ttcagaatta cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt
    42601 tccaaacaac tttgaccaac aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac
    42661 aagtcgtacc tcaattgacg gctgtattta gctaatcaaa tggtagtatg aagcattcaa
    42721 aatgaatgga taagcgattg atataaaaag aaacatagaa catactaaag gccgaagaaa
    42781 tggaggagaa taacagtcaa ttatgactct tgttcttcct cctttttacg taaaagcctt
    42841 ttaagtaaaa taaaacggat agaaaagaga acatatatga actattattc aaaaaataag
    42901 gaaaactttt atcaaaaact gaccgatgat ccgctttttt cttcattgac cgattatcta
    42961 tatgaacatc gagaacaaga aacgattcta agagaattga aaaaagaatt ttcgcaaaat
    43021 aaattttccc attttcttga tttattgatc gatgcaggat taatcaagag ggaagaacga
    43081 agatatcatt tgaactttcc aatatttgat cctaaagatt acttgcaaca agccacttcg
    43141 gctgctgaaa ccatagctga acaattgaag agactctctg ttgatgaaca aaaattagcg
    43201 atgggagaag ttatttgggc gtattgtttc gaagatgaac ggaaagaagc gtatttttat
    43261 ggagtaagaa acagccgaga gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac
    43321 cgttttatca cactttcctc tatagaacac ttcccgctta ctttggctaa ttactttttt
    43381 gtacagaaga atcagcttcc tgtaacgaag gcattcaaag aattagccga acttattgga
    43441 gacgtaaatg aatcgtactt ttttgatcaa atagaagtta tcgttgacag gatacgtaag
    43501 aataaatata aaaatcgccg gcctagtatt ttccatcagt ctctacttgt gacgaatact
    43561 ataaaggaag aagaatcatt cacattagaa ttacctattg tcgaaaaaaa caattttgag
    43621 attgaattac caacattgga tccttcattg acaatggagg aaatggcctt tcttaaacga
    43681 caaatctttt ctgagctgag caagaaattt ataccgcatg cattttctta tatcaaagaa
    43741 tatggaacag ttttagtgag taaaacctaa gagagcagta agcaaaaatt atactgactc
    43801 ttgcaggagg agcctttctc ttcctttatg gttaaaagaa ttatttgagt attttgccta
    43861 tctaccacct attagattca ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga
    43921 gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt agatttttaa ataatcaata
    43981 ttatgattca gtcaagtatg gcattttatg ggaagaacta gctgattaga ttaggaagtc
    44041 catttgataa tccagaatca ctaagaaacg aatggcgagt cattaataat ttatccaatc
    44101 ttgggttatt cttttagatt gaagtgttta aaactaaaaa agcatttatg aagtcgtaat
    44161 agtcaggctt caaaatgctt tttattttgt ttgttttttc tttgccacat aaatacacct
    44221 agactgacga tgaagagcat atccaacaga tttcctgcga cttggatcgt cgaagggttg
    44281 gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca
    44341 ggaactaatc gaaagaagag cggacatttc aattggctaa aaatgagtga aaaaataaaa
    44401 taagtaaaaa tggataaaat cacgactaaa ccattcatga taaagaagaa aagcgtattg
    44461 tgattaaacg aataaaaaag aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg
    44521 cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa aataggcacc gaaataaaca
    44581 gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa acggattttc ttcggcttct
    44641 gtgatatcag aatgatccaa aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc
    44701 ttaaggtatg aataacatag aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa
    44761 tgtaaaaaat tagtggttct acgttgaatt tccataatat ctccaaatta aattgataag
    44821 tatataaagt ataataacaa attgatgagt atatgtcgat gttttttaat caaaataata
    44881 aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt taagtttgtg
    44941 agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga aatatatttt
    45001 ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg atacagttca
    45061 cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc cagccccatt
    45121 gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa acttttatct
    45181 tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc caatttgtct
    45241 tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac aaataaattc
    45301 agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat cgtgatctcg
    45361 accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat gaaacgtaac
    45421 gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat ttttggatca
    45481 atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat cgtaagtgaa
    45541 gaaaaattga aactttatac atacaaaaaa atgataaaat gattataata gaattataaa
    45601 gataggttag gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct
    45661 aagtctcctt ctcagtatat acttaggtgt ctattcgatt caacagccaa ttgaggtaga
    45721 gaacattgtt tttcagatgt cgctgacaga tggtttaatt ttaagcgttt ttttcttttt
    45781 gatactgttt attggtaata ttttcggtag cgttgccagt tttttgaatt tttctattta
    45841 tccactcctt tcgctagcat taggagtggt gggactttgt tctctgtgct tatttcccga
    45901 gccattcagt cctgcattta tattatttgg tacactgaac ctgttccaga caactgttgg
    45961 agcatggttg ctatggcgat caggaaattt aatgaaaata ggtgagtaac aattgaatcc
    46021 aattatattg aaacgttctg tccatcatat tccagtgtta gaagttgttc tgaaagaatt
    46081 aagagaggaa cgtattcccg ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt
    46141 agttttgacg caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc
    46201 gatgaatcat ggggagcgca aacagccggt ttcttctatt ccatcctata cattttggga
    46261 tagtatctac ggaaatctgt ttgaatttga cacacttatc aagcatttag aaaaaagagg
    46321 tcttctacat gatcagcttg cagttggcgg ggtttcgatg gggggaatga caacatgcgc
    46381 cttgttggct aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc
    46441 tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa
    46501 agactatttt gatttgattt catgggttaa gagctatgat ttgtcattgc agccggaaaa
    46561 gttagctgga cgtcctctgt tcttttggca tggacaagct gacgaaaaaa tcccttatat
    46621 acaaacggaa cgatttgtag aaaaaaatcg gcataagtct tacggaaaag aaattgtttt
    46681 tcgttcctca aaaacacagc gacatatggt acaaatacca ctaatggaag aagcttccac
    46741 ttttttagca aaaaatttga taaaaaagta taaataattg aaacggaaaa tcactcatga
    46801 cgatagagga ttttccgttt tttattttgg gctataataa actctgttag gaaaatttgg
    46861 tataattgat agtaagaatg acaacatgtt agaaaggaag agagcatgcc cactttagca
    46921 gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat caatcatccg
    46981 gaacaggtga ctgacgaatt gaaagagtta gttttctcag caatggctga actggattac
    47041 cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat caaattattt
    47101 atactggaag aaatcgacac aactgagccc tattacatga atttgctgat gggaatcgct
    47161 aaacggatcg gtaaagcgca ttattctctc caattagtta caaatgatgc cttcgacgtc
    47221 ggttcttgtg atggatacat catcacaggt gtcagaaaaa aagacttcga ttggatcgac
    47281 cgtttggaaa aaccagttgt cttgtttggg gaaaattctg aaggatatga ttatgtagac
    47341 agcgataatg aatatggtac agcaaaagca acgcaatatg ccttagaaag agattatcaa
    47401 acgattattt atatcggtat cgaagtggac gagccttttg aaatttcaag ggaaactggt
    47461 tatttatctg tgatgaagga agttgatctt cctagtaaaa tcttgcggtt tccaaatcat
    47521 tcgacagaag tagaaagata tatgacagaa aactgggcag attttacaga taaaacttgt
    47581 tttgtatgta gttcagatcg gctagcacta ggagcagtaa gagggattat aaatgctgga
    47641 ggacacttgc ccgaagaatt tggtgtgatt ggatttgatg gggtattttt agaccaaatc
    47701 agttcaccta aattgacaac gatcaagcaa gatattatac gtatgggtga agtatgtgga
    47761 gaaatgttgt taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt
    47821 cctgaactag ttatcagaga gacaacgaat taagtcttat tactatatat atatttatga
    47881 aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg
    47941 ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga aatcagacga
    48001 aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac caacgacaag
    48061 aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca gtccccgttt
    48121 gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt
    48181 cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa gcataaagga
    48241 aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg acgaaaaaaa
    48301 ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga gtccaagcat
    48361 actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt tgttcctaaa
    48421 tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga ggatcattta
    48481 atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga accgacgccg
    48541 tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta
    48601 gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc
    48661 tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag ctattttaaa
    48721 taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc ggcgtttcct
    48781 tattttatac tctatccgaa agtttatatc gtatcctcaa atttttttga gtttaagcga
    48841 attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac tattttcgca
    48901 gatcgtttgc aaaaatagtt tattttttta taaagaagat tttactgaat aatatcattt
    48961 tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa gaatcagtgc
    49021 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
    49081 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
    49141 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
    49201 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
    49261 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
    49321 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
    49381 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
    49441 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
    49501 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
    49561 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
    49621 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
    49681 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
    49741 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
    49801 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
    49861 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
    49921 gtccaga
//
LOCUS       DACETN010000251        20863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.045, whole genome
            shotgun sequence.
ACCESSION   DACETN010000251 DACETN010000000
VERSION     DACETN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.045"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            267..1304
                     /locus_tag="IXF98_002766"
     CDS             267..1304
                     /locus_tag="IXF98_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1556998.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IXF98_002767"
     CDS             complement(1393..1716)
                     /locus_tag="IXF98_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1556999.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IXF98_002768"
     CDS             complement(1703..2410)
                     /locus_tag="IXF98_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1557000.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IXF98_002769"
     CDS             2496..2837
                     /locus_tag="IXF98_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1557001.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IXF98_002770"
     CDS             complement(3008..3856)
                     /locus_tag="IXF98_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1557002.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IXF98_002771"
     CDS             4454..5317
                     /locus_tag="IXF98_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1557003.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IXF98_002772"
     CDS             5417..5899
                     /locus_tag="IXF98_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1557004.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IXF98_002773"
     CDS             complement(6162..6629)
                     /locus_tag="IXF98_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1557005.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IXF98_002774"
     CDS             6752..7603
                     /locus_tag="IXF98_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1557006.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IXF98_002775"
     CDS             complement(7854..9812)
                     /locus_tag="IXF98_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1557007.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IXF98_002776"
     CDS             complement(9809..10681)
                     /locus_tag="IXF98_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1557008.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IXF98_002777"
     CDS             complement(11023..11895)
                     /locus_tag="IXF98_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1557009.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IXF98_002778"
     CDS             12023..12592
                     /locus_tag="IXF98_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1557010.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IXF98_002779"
     CDS             12659..13396
                     /locus_tag="IXF98_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1557011.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IXF98_002780"
     CDS             13627..14895
                     /locus_tag="IXF98_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1557012.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IXF98_002781"
     CDS             15382..15846
                     /locus_tag="IXF98_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1557013.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IXF98_002782"
     CDS             15830..16459
                     /locus_tag="IXF98_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1557014.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IXF98_002783"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IXF98_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1557015.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IXF98_002784"
     CDS             18338..18784
                     /locus_tag="IXF98_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1557016.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IXF98_002785"
     CDS             complement(18983..19498)
                     /locus_tag="IXF98_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1557017.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="IXF98_002786"
     CDS             19615..20817
                     /locus_tag="IXF98_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1557018.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACETN010000252        11428 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.085, whole genome
            shotgun sequence.
ACCESSION   DACETN010000252 DACETN010000000
VERSION     DACETN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.085"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            39..977
                     /locus_tag="IXF98_002787"
                     /pseudo
     CDS             39..977
                     /locus_tag="IXF98_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IXF98_002788"
     CDS             980..3163
                     /locus_tag="IXF98_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1557019.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IXF98_002789"
     CDS             3160..4161
                     /locus_tag="IXF98_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1557020.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IXF98_002790"
     CDS             4176..5090
                     /locus_tag="IXF98_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1557021.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IXF98_002791"
     CDS             5335..5451
                     /locus_tag="IXF98_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1557022.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IXF98_002792"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IXF98_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1557023.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IXF98_002793"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IXF98_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1557024.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IXF98_002794"
     CDS             9421..10683
                     /locus_tag="IXF98_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1557025.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IXF98_002795"
     CDS             10928..11410
                     /locus_tag="IXF98_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1557026.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3772 a         1910 c         2388 g         3358 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aaatccct
//
LOCUS       DACETN010000253         8182 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.102, whole genome
            shotgun sequence.
ACCESSION   DACETN010000253 DACETN010000000
VERSION     DACETN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.102"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(134..577)
                     /locus_tag="IXF98_002796"
     CDS             complement(134..577)
                     /locus_tag="IXF98_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1557027.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IXF98_002797"
     CDS             complement(795..2135)
                     /locus_tag="IXF98_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1557028.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IXF98_002798"
     CDS             2404..3300
                     /locus_tag="IXF98_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1557029.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IXF98_002799"
     CDS             complement(3450..4391)
                     /locus_tag="IXF98_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1557030.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IXF98_002800"
     CDS             4517..5638
                     /locus_tag="IXF98_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1557031.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IXF98_002801"
     CDS             5896..7398
                     /locus_tag="IXF98_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1557032.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IXF98_002802"
     CDS             7465..7992
                     /locus_tag="IXF98_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1557033.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2720 a         1516 c         1610 g         2336 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg ga
//
LOCUS       DACETN010000254         5926 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.115, whole genome
            shotgun sequence.
ACCESSION   DACETN010000254 DACETN010000000
VERSION     DACETN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.115"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            90..350
                     /locus_tag="IXF98_002803"
                     /pseudo
     CDS             90..350
                     /locus_tag="IXF98_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            513..1178
                     /locus_tag="IXF98_002804"
                     /pseudo
     CDS             513..1178
                     /locus_tag="IXF98_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1214..1459
                     /locus_tag="IXF98_002805"
     CDS             1214..1459
                     /locus_tag="IXF98_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1557034.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1562..2431
                     /locus_tag="IXF98_002806"
     CDS             1562..2431
                     /locus_tag="IXF98_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1557035.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2412..3146
                     /locus_tag="IXF98_002807"
     CDS             2412..3146
                     /locus_tag="IXF98_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1557036.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3179..4087
                     /locus_tag="IXF98_002808"
     CDS             3179..4087
                     /locus_tag="IXF98_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1557037.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4084..4626
                     /gene="sat4"
                     /locus_tag="IXF98_002809"
     CDS             4084..4626
                     /gene="sat4"
                     /locus_tag="IXF98_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1557038.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4719..5513
                     /locus_tag="IXF98_002810"
                     /note="aph(3')-IIIa"
     CDS             4719..5513
                     /locus_tag="IXF98_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1557039.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5621..5809)
                     /locus_tag="IXF98_002811"
                     /pseudo
     CDS             complement(5621..5809)
                     /locus_tag="IXF98_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5789..5914
                     /locus_tag="IXF98_002812"
                     /pseudo
     CDS             5789..5914
                     /locus_tag="IXF98_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         2048 a          882 c         1283 g         1713 t
ORIGIN      
        1 gattttgtac aacccatcag tccttattga caaagagccg gaatttgtat cgataagggg
       61 tacaaattcc cactaagcgc tcgggacccc ttgtaggaaa atgtcctaag tgtggcaaca
      121 atattgtatt aaaaaaatcg ttttatggtt gttcaaatta tcctgaatgt aagtttactt
      181 tagctgaaca ttttagaaag aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg
      241 gaaaagaaac cctggtaaaa ggaatcaaaa acaaagagaa aaagccctac aatgccgttg
      301 taaaaattgg ggaaaaggga tatattgatt ttatctcttt ttcaaaataa acatggggct
      361 gacccaaaag tagctcctat atgaaaaata gaggtctttc catttagata gtagtggaaa
      421 gacctctatt tatcttttga aagtgaatac aaaaataaag cacactctgc tataatatag
      481 gtgattaatc catttgagag aggtgcttta tcatgtatca aaattatacc ataaaccaac
      541 tttgtttgcc tatagatctt gaaattaaat tagaagaaaa tgattttgct catgccattg
      601 tgcagtttgt tgactctatt cccgatgaag tctttcttcc ttattatcaa tctatgggta
      661 gaccccaata tcatcctcgt atgctacttt ccattattct ttgtgcctat attcaagggg
      721 tttattcggg ccgccaaata caaaatatgc tgatagatag tattcggatg cgctacttat
      781 ctcaagagca attccctaac tttcgtacta ttaatcgttt tcgcgttcat ccgattatga
      841 acgatattct agatcattca tttgttcaat ttcgtgagct actggttcaa agtggcttaa
      901 tcagtggcag tgctctatac attgatggta caaaaattga agcggatgcg aataaatact
      961 ctttcgtttg gaaaaaaagt attttaaagt ataaggatag ccttgatcaa aaagcccttg
     1021 aaaactacca acaaatggtg gaaacaaaaa tcttacctga gttaatagat gatttaaagg
     1081 atgaactttc tatagaagag attgaaagga ttcgtcaatc tttggaagaa aaggaaaatc
     1141 aattaattga agcgattgac caaaccgaaa cagtagaagg gggctgaccc attattaata
     1201 aggagtggac aacatgattg atgtaaatga gctattggaa gaagctatta gagaaactga
     1261 aaatctaaat gatggcgaag tttttcttgt taaagattta ttcaagggat atgtggggaa
     1321 taggataccc agaaaggatc gacttctgct tggaacgtta tttttaaacc atgttagcaa
     1381 aatgaatggt aatttaaagg ctattgaaaa gacttcatct aatcagcaga ggtataaaaa
     1441 gacaattgat aagttttaac tatcttttgt gaaattaaaa aaattataaa tatgttgagt
     1501 agaagcaaaa gatttggctt ggaattttta cagcagtgaa tcgataaaag gagcatgctt
     1561 gatggtagat aatattatta aatcagtagc agagaaatta tcctctctgt cttatataga
     1621 aggtattgtt ttaggtggtt cacgtgcaag gggcacccat acagaggatt cggatataga
     1681 tatcggcatc tattacaatt cagaatcatt tgacataaat actattaatc aattcgctac
     1741 aaagctggat gatgagcata gaaataacct tgttgtacct cccggagcat ggggtgattg
     1801 gattaatggc ggcggatggt tagtcataaa cgggtatcat gtggatttaa ttttacgtga
     1861 tattaaacgt gtggaacaaa taatgaaaga tacagagcac ggaattgtta ctgccaatta
     1921 tcagactggg catccccatg gttatattag tgcaatgtat cgaggagaat tagcgattag
     1981 caaaatacta tatgctaaga atgaaagctt atgcgaatta aaaaaacagg cagaaactta
     2041 tcccaatgct ttgcagaaaa gtttagttaa cttttttatg tttgaagcag ggttctcttt
     2101 aatgtttgta aaagcaaatt cgggaacaga cgataaatat tatattgcgg gtcatgtttt
     2161 tcgtatagtt tcatgtttaa atcaagtgtt atttgcatgt aataatgctt attgtatcaa
     2221 cgaaaagaaa gctataaaac tgcttgaaac ttttgaacat aaacctgaaa aatataccga
     2281 gaaggtaaat catatttttg aagtactcgg tatctcactt tttgaatgct acgacatgac
     2341 cgagaagctt tataatgaag tgaatgaaat tgtatcggag ataaataact ttttaaacga
     2401 ggagagttca gatgaaagaa aacaaatatg atgataatat attttttcaa aaatacagtc
     2461 aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact ttgaaaaaga
     2521 tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat ggatggcact
     2581 gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa
     2641 tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa tgctgtgcca
     2701 tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg cttgcgtttc
     2761 attatgtagc agactatgag aatttaataa aaaagatata taggatgctg aaggctggtg
     2821 gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca caagactggt
     2881 attataacga aaaaggagaa atactgcatt tcccggtgga caattattat tatgagggca
     2941 aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca ctgaccacat
     3001 atctaaatac actgctttca aatagtttta taataaatca gattgtggag ccacagccgc
     3061 cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca atgatgctga
     3121 ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga gtatgtaaat
     3181 gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg atgaacgtat
     3241 tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag atgaatttca
     3301 ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta atgatgactg
     3361 gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg aattattccc
     3421 acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata aaattgatct
     3481 taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat taataaaggt
     3541 tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata tagattatca
     3601 tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt ggaatgtaac
     3661 accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg atcattttaa
     3721 tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca tcgaaacagg
     3781 ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat ccgaggattt
     3841 gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat gggaagcatt
     3901 atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa ggcttcatta
     3961 tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt ataaaaaata
     4021 cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag agaggcggga
     4081 acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc cagcgaacca
     4141 tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa
     4201 ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag
     4261 gaggcagatt gccttgaata tattgacaat actgataaga taatatatct ttactaccaa
     4321 gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta cgcttatata
     4381 gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat
     4441 atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac
     4501 aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat
     4561 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     4621 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     4681 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     4741 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     4801 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     4861 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     4921 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     4981 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     5041 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     5101 cggattgtcc ctatacgaat agcttagaca gccgattagc cgaattggat tacttactga
     5161 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     5221 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     5281 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     5341 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     5401 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     5461 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     5521 atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt
     5581 acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagataga
     5641 gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg gcttttataa
     5701 ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg atatactttt
     5761 tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa cctctattct
     5821 tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc gatttaatat
     5881 aagcttaagg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DACETN010000255         5852 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.117, whole genome
            shotgun sequence.
ACCESSION   DACETN010000255 DACETN010000000
VERSION     DACETN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.117"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            322..1014
                     /locus_tag="IXF98_002813"
     CDS             322..1014
                     /locus_tag="IXF98_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1557040.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="IXF98_002814"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="IXF98_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1557041.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="IXF98_002815"
     CDS             complement(1910..3862)
                     /locus_tag="IXF98_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1557042.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4165)
                     /locus_tag="IXF98_002816"
     CDS             complement(3869..4165)
                     /locus_tag="IXF98_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1557043.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="IXF98_002817"
     CDS             4507..4797
                     /locus_tag="IXF98_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1557044.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5511..5813
                     /locus_tag="IXF98_002818"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="IXF98_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1998 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca ta
//
LOCUS       DACETN010000256         2318 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.164, whole genome
            shotgun sequence.
ACCESSION   DACETN010000256 DACETN010000000
VERSION     DACETN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.164"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(49..1152)
                     /locus_tag="IXF98_002819"
     CDS             complement(49..1152)
                     /locus_tag="IXF98_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1557045.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1705..2253
                     /gene="aac(6')"
                     /locus_tag="IXF98_002820"
     CDS             1705..2253
                     /gene="aac(6')"
                     /locus_tag="IXF98_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1557046.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          737 a          413 c          449 g          719 t
ORIGIN      
        1 tgattttgca caactcatca gtccttattg acaaagagcc gcaattattt aactgatttt
       61 cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg
      121 tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc
      181 tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac
      241 tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga actgctgatc
      301 catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat
      361 tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt
      421 gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat
      481 tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag
      541 acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc
      601 gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca
      661 taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg
      721 ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc
      781 agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt
      841 ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc
      901 tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc
      961 cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt
     1021 ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt
     1081 gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac
     1141 actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt
     1201 gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg
     1261 aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca
     1321 aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct
     1381 ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt
     1441 ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa
     1501 caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt
     1561 gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat agagatactg
     1621 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     1681 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     1741 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     1801 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     1861 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     1921 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     1981 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     2041 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     2101 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     2161 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     2221 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     2281 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DACETN010000257         1218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            1 Isolate 77 SAMN10983743-rid9591643.guided.197, whole genome
            shotgun sequence.
ACCESSION   DACETN010000257 DACETN010000000
VERSION     DACETN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983743
            Sequence Read Archive: SRR8602267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705481.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:18:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 25 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983743-rid9591643.guided.197"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 1 Isolate 77"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            111..194
                     /locus_tag="IXF98_002821"
     CDS             111..194
                     /locus_tag="IXF98_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1557047.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IXF98_002822"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IXF98_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1557048.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          453 a          195 c          199 g          371 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgggctcta taatttatgg
     1201 gactgatgaa tcagaatt
//
